Rh2BG318900

positive regulation of cell-substrate adhesion

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000307
Physical Location & Seq
Forward (+)
37992983 .. 38002010
9028 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG318900.1

Sequence Viewer

Length: 5157 bp
ATGCCATTGGAAGATGACCCGTTCTTAACTATGGGCAACCTACGATTGCTGAAGATTTGTAATGTAAATTTTCTGGATGTGCACTTCAGATATGTCTCTAAGAATTTACGACTTTTGGAATGGCACGAATGCCCTCTAGTATCTCTGCCATCTAGTTTTAAATCAGACAAGCTGGTTGAATTAAAGATGCCTAACAGCCGCATTGAACAACTATGGAATGAAACGCTTTCTCTGAAAATGCTTATACTCATGGATCTTTCTGACTGCAAGTATTTAACCAAGACCCTGGACTTCAGTAAGGTCCCAAAGCTTGAGAAATTGATCCTTAAAGGTTGTATAGAGTTATTGGAGGTTCACCCTACTATTGGGGATCTCCAACATTTGGTTTTATTGAACTTGAAAGGTTGTGAAAGTCTAGAGAACCTTCCTCAATCCATCAGGTTGAGATCTCTTCGAACTTTTATTCTTTCGGGATGTTCAAAACTCAGACACTTTCCAGAGATTGTGGGGAACATGGATACTTTATCAGAACTTTATTTAGATGGCACGGCTATACGGGAGCTGCCTGTATCAATCCAGCATTTGGAAGGCCTCATTTTACTTAATCTAAGTGGGTGCAGGAACCTTCTCAGTGTTCCAAGCATCCTTTCTAGTAGTTTGACATCACTGAAATTTCTCTATCTGTCACTATGCTCACGTATGGACAGACTGCCGGATAACATAGGCTGCTTGGAACACTTGGAGGAGCTTGATGCGTGTAATATTGCTATAAGAAAAGTGCCCGATTCCATTTTTCTCCTCAAGAATCTTAAATTATTGTGTTTCCATGGCTGTGCTGGATCCACAGGTTTAGAGTTGCCAAGCAAGTTCTCAGGTTTAAGATCTTTGACAACACTAAACCTAGGTGGATGTAATCTCGCAGAAGGAGCAATCCCCAATGACATTGGTGATTTATTCTCACTGCAGAGTCTGGATTTAAGTGAGAACAACTTTTTCACCATACCTGAAAGCATCTCTCAGCTTTCTGAGCTTACAGAAATTTCTCTGTTTAAGTGTAGCAAACTATGGACGTTGCCAAAAGATCTTCCGTCAAGTCTAAGAAATTTAAATGTACGAGGTTGTCCTATGCTGACAAGTTCTTCATCTAGTTGGAGGAGATATCCGCCTCAAAAGGGTTTGAGTATCATAAACTGTCGAAAACCAGAGGAGGATGAAATCTTTCCCGAACTCTATAAGTTTCGTTTGGGAAATCTGGAAGAACTCGATCTTTCCAACTGCCAACACTTGAAGAAGATCCCTGACTTGAATGGGGTTCCAAATCTTAAGAAATTGAACCTTGAAGGTTGTGAAAAATTATCAGAGGTTCACCCAACCATTGGGTGTCTCCAACATTTGGTGTTTCTGAATTTGAAAGGATGTGTAAATCTAGAGAGCCTTCCCAGTTCCATCAGCTTGAAGTCTCTAAACATGTTTGTTCTTTCGGGATGTTCAAAGCTGAAAGAGTTTCCAAACATTAAGGGAGAAATGAACAATTTGTCACAACTGCATTTAGATGGGACGGCGTTAAGGCATCTGCGAATTCCGATGATGCATTTGAAAGGCCCAATTTTGATAAATCTGTCAGGCTGCAGGAACCTTTTGACTGTTCCAATCCTATTCAGTCTAAAATCTCTCAATCTATCACGGTGCTCAACTATATTCAAATTGCCACCGAGCCTGGCATACATGGAACATTTGGAGGAGCTTGATGCCTCTGAAACTGCTATAACAGGATTACCCCAGTCCATTTTACTCTTGAAGAAACTTAAAGTGTTATCTTTCTGTGGATGCAAAGGTTTGCAATTGCCTAAATGGTTCTCGGATTTAAGCTCTTTGACGTCATTAAATCTACGGAGGTGTAGTCTAGCAGAAGAAGTCCTTGATAGCCTCTACAGCTTATCTGCTCTGCAGATTTTGGATTTGAGTGAAAACAATCTTTCGATCATACCCAGTGGAATTGGTCGCTTATCCTCTTTGAAGCTATTGAATTTGAGTGAGAACAATTTTGACAATATACCAAATGAAATCGGTCACTTATCCTCTTTGCAGGTCTTAGATTTGAGTGAAAACAGCTTCATGAGTATACCTGATGAGAGCATCTCTCATCTTTCTGAGCTTACAGAACTACGCTTGTTTAGATGTAGGAAGCTACAGTCCTTGCCGAATAATCTTCCCTTCAATCTAAAACATGTCCATGCACGAGAATGTCCTATGTTGAAGAATAATGCAGATACTTTGACAATATGGGCATCTGGAAAGGGGTTCTGTTTCATAAATTGCAGACAATCTGACCAGGATGATGGTCAGCCAAATCACCTCCCAGTTCCAGTTCCCAAAGACCGTATTGAACTACTCTTTCCTTCATACATTGAGGACCGAGTGTATGGCAAAAAACCATTTGAAATTCGTTTTCCACATTCGTGGAGTACAGGACTGAGTGCCGGAATTCCAAATTCGTGGAGTCGTTGGAGAAATGGCCCTTCCGTGACAATCCCACTCTCTGATGGTAACAGTACGTGGATGGGACTTGCTCTTTTTGTTGTTTTTGATATCCTTGAGCAAGACATTTTCAACAAGAGCTGGGAATTGGAGGAGACAGTCTGTGATTTTCACACCGACGTTGGGGCGGTTGGGCATGATACTTCCCTAGTTTTTCAAAACTTCATAGACTTCAGGACTGGGTCATATGGTCTTAGTTGCTATGAACCACGTGGTGGGCAATTTAGTGGGTTGTTCGATAAACCAAGTTCACGACTTCGAGCTTCAGTTTCAACAAAGAGACCAAATTTAAAAGTTAGAGGTTGTGGGATACATCTAATATCAGAGGAATATGCTGCAGAGTTTGTTAAAAGTATAGCAACTCAGACTACTAATTCAATTGAGAGTCATCTTGATTCCAATTTTGATCGACATTGCGAGGACATATTAGATGAGGAAACAACTGGTGTCCTGATAGAACAGGGATCTACTAATTCAACTTTCAATGAAGATAGCTGCAGCAAAGTTAACTCTAAGATCAAAATCAGAGGAGAGCTGTCGATACTCTATGAGGGAAGCAAGGGACGTCAGAAGAGTTTCCACTTTTGCTTTCCTGCTTCAGTAATTTCAACCCTGCCTTGGTTCTTCCATCACCATGCAGGGGATGTAACATTGTGTTACATCACTAAAAACTTACTTGATGATCAGAGATGGGTGGGATTAGAACTATATGTTCAATTTTCTCGGTGTACATCTACTTCGACATCTGGCAATAGTAGTTTTTTCTTTTATGTTGATTTATGCTCTCATGATCATGGAAGTATGGTAATGCACGGATCACTCAAGATAAAGAGTTGTGTTGGGACCTCAGATCAACTTGTTGTATTACATGTACCACGTGTTCATTTTCAACAACAGTTGAATCAATGTCAGGGTATCAGCGCATTGTTCAGAACCATTAATGAAGAAATGGAGGTCCAAGTGTGTGGAAGCCGTTTAGCATTTGAGCATGATTTGGAAGACTTGACCCATTCATTAACTGCTGCTGCCAGCACGTTGGGGCAACATGCCCTCACTCAACTATGTTCCCAGGCCCAACCTGTTGATCGGCGCAATGCAGAGGAAGCAGAAATGCCCTTCAATTGCTGTTCCTGGTTTAGAAGAAGTACTGCACTTGTACTACCAGAACAAGCTCCTTCTTCTTCAATTGTGCGGCTGCGAAATCACAGATGCTATCTCCAGCAACAGCAAGGTTTTGGGGAGTCTGACCTTACCCAATTGGTCCACTCCAGATCAGTAACATTACTCCACTCGAAAAGCCTTTTGGAAAACAGGGATAGAAACCATGAAAACGTTGCCGAAGAAGATAAATCGTTGGTGTTGGCTCGTCATCATGTACATATAATGGCTGAGACTCAACTTCAGGGAAGATATGGTTCACCACGGTGGAAGAGATGCCTGAAGTTGTTGCTTCGACAGTCCAAGGTGGCTACTCTCAGTCTTTGTGGACACGCAATTTCAGCTTTCAAGAATTTTGATCCTTTCTCCCCATACAATATTATTTGTTTCTCTGACAAGGAAATTCCAGTGTGGTTCAAACATGAGATGTCGTATCAGATGTCTTCTAGGTCTAGGGTGGGAATCAAACTACCTCCAAGATTGCACGAGGATGAGAACTGGAGAGGACTTGCTATATGTGTTGCCTTTGAAGTTCATGACCAGCGTCCAACTACCTCCCCGGTCAAACTTCTCTGTCACTTGAGAGCCAAGGACAACTATTGCTTGAATCATATCCCTATGTGTTGCATCAATGAAGAGAAACTCAAGTCGCTGCATCTTGGTAGATTCATTTGGCTAACCTACATTCCCCGTCTTTTGCTAACGGAGTTCAGTGTGATTAGTGATGTAGAGGCCAGAATTTATGTTAGTTGCAGAGGCTTGACAGTAGAGAAGAGTGGTATACGTCTCTTGTACAGGCAAGAAGAGGGGGAGTTTGAGAACACAATAACCGAGTGCTGGACATCTTTCTTTGATAATCTGTCTTTCATCCGTCAACTAGTAGAATCAGATGATCAAAATATTCAACCAAGGATAAGACATGAACTTCCGATGCTTGAAGGTCATATCAAGGTTTTTGAACCGGATTTAATATATAATGCAATCCCCCCGTCTAATGAAATTCCGGAGTGGTTCGGGCACCGCATTGAGGACCCCTGGGATCCCAGCTGTGGCTGGCAATTCCAGTTACCACCACCTTTGAGTGACACAATAGGATTAGCTCTCTTTGTGTCATATCGTATTAGCAGAAATTATTTGCAGGAAAGTGTTTTGTACCCCTTTATTATAACATTGAAAACTGCGAAGAATGGTTTGTCCTCTCTCCATCGGTATCAAATGAGCAATGAAGAATATGAGTTCCTAAAGCGCTGTTGTACTGTGCATCGTGAATTTTTCTGGCTCTCCTACATACCACGACGCTGGTTTCTACATCAACTAAATGATGAGTCTGACCTCATAGTTTTTTCTGGTCGGAACTGCTGGACGCCGGACATGGTCTATCTCCGTTTTGTGTATGCGGATGAGGTGGAAGAGTTTAAGCAGCTCTGTTTCAATCTTCATCGACCCCCTGTCCAACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04064 ko04115 ko04210 map04064 map04115 map04210
Pfam Domains
Protein Families
Death LRR_8 Peptidase_S68 ZU5

Protein Analysis

1718

Amino Acids

195.41

Weight (kDa)

6.38

Isoelectric Point (pI)

47.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 119 - 226 6.3e-07 Leucine-rich repeat region
LRR_13 PF23286 149 - 216 3.4e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 201 - 301 4.2e-10 Leucine-rich repeat region
LRR_14 PF23598 406 - 517 4.9e-06 Leucine-rich repeat region
LRR_4 PF12799 417 - 454 1.6e-06 Leucine Rich repeats (2 copies)
LRR_14 PF23598 539 - 655 2.6e-11 Leucine-rich repeat region
LRR_14 PF23598 637 - 735 4.9e-12 Leucine-rich repeat region
LRR_8 PF13855 671 - 727 1e-07 Leucine rich repeat
C-JID PF20160 1363 - 1500 3.8e-07 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000307)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g16600 FvH4_1g16610 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16621 FvH4_1g16630 FvH4_1g16640 FvH4_1g16640 FvH4_1g16650 FvH4_1g16650 FvH4_3g27631 FvH4_4g11392 FvH4_4g11542
prunus_persica Prupe.4G224500_v2.0.a1 Prupe.4G226900_v2.0.a1 Prupe.4G227000_v2.0.a1
pyrus_communis pycom11g18180 pycom11g18210 pycom11g18230 pycom11g18270 pycom11g18350 pycom11g18360 pycom11g18370
rosa_chinensis RchiOBHm_Chr2g0106511 RchiOBHm_Chr2g0106541 RchiOBHm_Chr2g0106551 RchiOBHm_Chr2g0106571 RchiOBHm_Chr2g0124621 RchiOBHm_Chr6g0251591 RchiOBHm_Chr6g0251631 RchiOBHm_Chr6g0251641
rosa_laevigata RLG00000015443 RLG00000015444 RLG00000015458 RLG00000017510 RLG00000017511 RLG00000017512 RLG00000017515 RLG00000017516 RLG00000017517 RLG00000018809 RLG00000020934 RLG00000020935 RLG00000034092
rosa_multiflora Rmu_co8285777.1_g000001 Rmu_sc0000762.1_g000001 Rmu_sc0001872.1_g000006 Rmu_sc0005255.1_g000019 Rmu_sc0005597.1_g000006 Rmu_sc0012104.1_g000001 Rmu_sc0017105.1_g000011 Rmu_sc0022904.1_g000001 Rmu_ssc0000135.1_g000049 Rmu_ssc0000213.1_g000047
rosa_roxburghii Rroxscaffold_2G00092830 Rroxscaffold_2G00119790 Rroxscaffold_2G00137010 Rroxscaffold_2G00137030 Rroxscaffold_2G00137070 Rroxscaffold_7G00216560
rosa_rugosa Rorug02G0136100 Rorug02G0136200 Rorug02G0136300 Rorug02G0136400 Rorug02G0251800 Rorug02G0251800 Rorug02G0455500 Rorug02G0455600 Rorug02G0455700 Rorug05G0195300
rosa_samantha Rh2AG187400 Rh2AG187500 Rh2AG187600 Rh2AG310500 Rh2BG198200 Rh2BG198300 Rh2BG198400 Rh2BG198600 Rh2BG318900 Rh2BG534300 Rh2CG192400 Rh2CG192500 Rh2CG192600 Rh2CG297500 Rh2DG193500 Rh2DG193600 Rh2DG193800 Rh2DG334200 Rh2DG543100 Rh5AG280400 Rh5BG285800 Rh5BG285900 Rh5CG317900 Rh5CG318000 Rh5DG294600 Rh5DG294700 Rh6AG012200 Rh6AG012700 Rh6BG011200 Rh6BG011700
rosa_wichuraiana Rw0G009380 Rw2G014610 Rw2G014630 Rw2G014640 Rw2G014680 Rw2G025120 Rw2G043000 Rw5G026490 Rw6G001370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 4862
AatII GACGTC 2 cut(s) 1880, 3106
Acc36I ACCTGC 1 cut(s) 2077
AccB1I GGYRCC 1 cut(s) 4713
AccB7I CCANNNNNTGG 5 cut(s) 382, 583, 1376, 1393, 2753
AccI GTMKAC 2 cut(s) 2122, 4477
AccIII TCCGGA 1 cut(s) 4699
AciI CCGC 6 cut(s) 199, 1163, 2666, 3730, 4717, 5093
AclI AACGTT 1 cut(s) 3870
AcuI CTGAAG 8 cut(s) 70, 71, 277, 2695, 2787, 3120, 3923, 3998
AcvI CACGTG 2 cut(s) 2750, 3416
AcyI GRCGYC 3 cut(s) 1877, 3103, 5060
AdeI CACNNNGTG 1 cut(s) 2753
AfeI AGCGCT 1 cut(s) 4943
AflII CTTAAG 1 cut(s) 1322
AflIII ACRYGT 4 cut(s) 1467, 2227, 3406, 3415
AhdI GACNNNNNGTC 1 cut(s) 5144
AhlI ACTAGT 1 cut(s) 4573
AjnI CCWGG 6 cut(s) 285, 1716, 2331, 3606, 3668, 4730
AjuI GAANNNNNNNTTGG 4 cut(s) 631, 663, 3824, 3856
AleI CACNNNNGTG 2 cut(s) 2458, 3961
Alw21I GWGCWC 2 cut(s) 84, 1691
Alw26I GTCTC 7 cut(s) 100, 1388, 1464, 2627, 2812, 3923, 4487
Alw44I GTGCAC 1 cut(s) 80
AlwNI CAGNNNCTG 1 cut(s) 970
Aor13HI TCCGGA 1 cut(s) 4699
Aor51HI AGCGCT 1 cut(s) 4943
AoxI GGCC 5 cut(s) 589, 1600, 2515, 3609, 4428
ApaLI GTGCAC 1 cut(s) 80
AseI ATTAAT 1 cut(s) 3477
Asp700I GAANNNNTTC 2 cut(s) 1218, 3113
AspA2I CCTAGG 1 cut(s) 901
AspLEI GCGC 3 cut(s) 3461, 3630, 4944
AspS9I GGNCC 9 cut(s) 301, 1601, 2413, 2516, 3381, 3493, 3610, 3799, 4726
AsuC2I CCSGG 1 cut(s) 4256
AsuHPI GGTGA 7 cut(s) 347, 959, 988, 1358, 2345, 3161, 3948
AsuII TTCGAA 1 cut(s) 454
AvaII GGWCC 6 cut(s) 301, 2413, 3381, 3493, 3799, 4726
AvrII CCTAGG 1 cut(s) 901
BaeGI GKGCMC 3 cut(s) 84, 783, 4716
BamHI GGATCC 2 cut(s) 839, 4735
BanI GGYRCC 1 cut(s) 4713
BarI GAAGNNNNNNTAC 2 cut(s) 3259, 3291
BauI CACGAG 2 cut(s) 2238, 4181
BbrPI CACGTG 2 cut(s) 2750, 3416
BbsI GAAGAC 2 cut(s) 3543, 4131
Bbv12I GWGCWC 2 cut(s) 84, 1691
BceAI ACGGC 3 cut(s) 564, 1575, 3495
BciT130I CCWGG 6 cut(s) 287, 1718, 2333, 3608, 3670, 4732
BciVI GTATCC 2 cut(s) 511, 2841
BclI TGATCA 3 cut(s) 3220, 3328, 4588
BcnI CCSGG 1 cut(s) 4256
BcoDI GTCTC 7 cut(s) 100, 1388, 1464, 2627, 2812, 3923, 4487
BcuI ACTAGT 1 cut(s) 4573
BfmI CTRYAG 7 cut(s) 962, 1627, 1930, 1946, 2189, 2874, 3034
BfoI RGCGCY 1 cut(s) 4945
BfrI CTTAAG 1 cut(s) 1322
BfuAI ACCTGC 1 cut(s) 2077
BfuI GTATCC 2 cut(s) 511, 2841
BglII AGATCT 3 cut(s) 446, 881, 1081
BlnI CCTAGG 1 cut(s) 901
BmcAI AGTACT 1 cut(s) 3685
Bme1390I CCNGG 7 cut(s) 287, 1718, 2333, 3608, 3670, 4256, 4732
Bme18I GGWCC 6 cut(s) 301, 2413, 3381, 3493, 3799, 4726
BmeRI GACNNNNNGTC 1 cut(s) 5144
BmgT120I GGNCC 9 cut(s) 301, 1601, 2413, 2516, 3381, 3493, 3610, 3799, 4726
BmiI GGNNCC 9 cut(s) 303, 623, 841, 1314, 1634, 3382, 4715, 4728, 4737
BmrFI CCNGG 7 cut(s) 287, 1718, 2333, 3608, 3670, 4256, 4732
BmrI ACTGGG 5 cut(s) 1434, 1774, 1983, 2354, 2727
BmuI ACTGGG 5 cut(s) 1434, 1774, 1983, 2354, 2727
BoxI GACNNNNGTC 1 cut(s) 4239
BpiI GAAGAC 2 cut(s) 3543, 4131
BplI GAGNNNNNCTC 4 cut(s) 2123, 2155, 2125, 2157
BpmI CTGGAG 3 cut(s) 3740, 3790, 4216
Bpu14I TTCGAA 1 cut(s) 454
BpuEI CTTGAG 6 cut(s) 332, 785, 2615, 3344, 4297, 4325
BpuMI CCSGG 1 cut(s) 4256
BsaAI YACGTR 4 cut(s) 698, 2556, 2750, 3416
BsaBI GATNNNNATC 1 cut(s) 3059
BsaHI GRCGYC 3 cut(s) 1877, 3103, 5060
BsaI GGTCTC 1 cut(s) 2812
BsaWI WCCGGW 2 cut(s) 4657, 4699
BsaXI ACNNNNNCTCC 2 cut(s) 1885, 1915
Bse3DI GCAATG 3 cut(s) 2950, 3637, 4924
Bse8I GATNNNNATC 1 cut(s) 3059
BseAI TCCGGA 1 cut(s) 4699
BseBI CCWGG 6 cut(s) 287, 1718, 2333, 3608, 3670, 4732
BseJI GATNNNNATC 1 cut(s) 3059
BseMI GCAATG 3 cut(s) 2950, 3637, 4924
BseRI GAGGAG 7 cut(s) 758, 788, 1168, 1220, 1754, 2645, 3081
BseSI GKGCMC 3 cut(s) 84, 783, 4716
BseYI CCCAGC 2 cut(s) 2619, 4739
BsgI GTGCAG 2 cut(s) 637, 3672
BshFI GGCC 5 cut(s) 591, 1602, 2517, 3611, 4430
BshNI GGYRCC 1 cut(s) 4713
BsiHKAI GWGCWC 2 cut(s) 84, 1691
BsiSI CCGG 6 cut(s) 713, 2481, 4256, 4658, 4700, 5063
BslFI GGGAC 5 cut(s) 287, 1570, 2577, 3114, 3394
BsmAI GTCTC 7 cut(s) 100, 1388, 1464, 2627, 2812, 3923, 4487
BsmBI CGTCTC 1 cut(s) 4487
BsmFI GGGAC 5 cut(s) 287, 1570, 2577, 3114, 3394
BsmI GAATGC 1 cut(s) 134
BsnI GGCC 5 cut(s) 591, 1602, 2517, 3611, 4430
Bso31I GGTCTC 1 cut(s) 2812
Bsp119I TTCGAA 1 cut(s) 454
Bsp1286I GDGCHC 4 cut(s) 84, 783, 1691, 4716
Bsp13I TCCGGA 1 cut(s) 4699
Bsp1407I TGTACA 3 cut(s) 3266, 3913, 4488
Bsp19I CCATGG 1 cut(s) 826
BspACI CCGC 6 cut(s) 199, 1163, 2666, 3730, 4717, 5093
BspANI GGCC 5 cut(s) 591, 1602, 2517, 3611, 4430
BspEI TCCGGA 1 cut(s) 4699
BspHI TCATGA 3 cut(s) 2115, 3325, 4231
BspLI GGNNCC 9 cut(s) 303, 623, 841, 1314, 1634, 3382, 4715, 4728, 4737
BspMAI CTGCAG 5 cut(s) 966, 1631, 1950, 2878, 3038
BspMI ACCTGC 1 cut(s) 2077
BspT104I TTCGAA 1 cut(s) 454
BspT107I GGYRCC 1 cut(s) 4713
BspTI CTTAAG 1 cut(s) 1322
BspTNI GGTCTC 1 cut(s) 2812
BsrDI GCAATG 3 cut(s) 2950, 3637, 4924
BsrGI TGTACA 3 cut(s) 3266, 3913, 4488
BssNAI GTATAC 2 cut(s) 2123, 4478
BssNI GRCGYC 3 cut(s) 1877, 3103, 5060
BssSI CACGAG 2 cut(s) 2238, 4181
BssT1I CCWWGG 6 cut(s) 826, 901, 3155, 3999, 4284, 4604
Bst1107I GTATAC 2 cut(s) 2123, 4478
Bst2BI CACGAG 2 cut(s) 2238, 4181
Bst2UI CCWGG 6 cut(s) 287, 1718, 2333, 3608, 3670, 4732
Bst6I CTCTTC 7 cut(s) 456, 3104, 3962, 4326, 4463, 4494, 5100
BstACI GRCGYC 3 cut(s) 1877, 3103, 5060
BstAFI CTTAAG 1 cut(s) 1322
BstAUI TGTACA 3 cut(s) 3266, 3913, 4488
BstBAI YACGTR 4 cut(s) 698, 2556, 2750, 3416
BstBI TTCGAA 1 cut(s) 454
BstC8I GCNNGC 2 cut(s) 3568, 4751
BstDSI CCRYGG 2 cut(s) 826, 3959
BstH2I RGCGCY 1 cut(s) 4945
BstHHI GCGC 3 cut(s) 3461, 3630, 4944
BstMAI GTCTC 7 cut(s) 100, 1388, 1464, 2627, 2812, 3923, 4487
BstMWI GCNNNNNNNGC 5 cut(s) 926, 1027, 1932, 3641, 4037
BstNI CCWGG 6 cut(s) 287, 1718, 2333, 3608, 3670, 4732
BstNSI RCATGY 4 cut(s) 1471, 2231, 3410, 3587
BstPAI GACNNNNGTC 1 cut(s) 4239
BstSCI CCNGG 7 cut(s) 285, 1716, 2331, 3606, 3668, 4254, 4730
BstSFI CTRYAG 7 cut(s) 962, 1627, 1930, 1946, 2189, 2874, 3034
BstSLI GKGCMC 3 cut(s) 84, 783, 4716
BstV2I GAAGAC 2 cut(s) 3543, 4131
BstX2I RGATCY 9 cut(s) 253, 370, 446, 839, 881, 1081, 1293, 3002, 4735
BstXI CCANNNNNNTGG 7 cut(s) 286, 2339, 2460, 2496, 3503, 3574, 4995
BstYI RGATCY 9 cut(s) 253, 370, 446, 839, 881, 1081, 1293, 3002, 4735
BstZ17I GTATAC 2 cut(s) 2123, 4478
BsuI GTATCC 2 cut(s) 511, 2841
BsuRI GGCC 5 cut(s) 591, 1602, 2517, 3611, 4430
BtgI CCRYGG 2 cut(s) 826, 3959
BtsI GCAGTG 1 cut(s) 959
BtsIMutI CAGTG 6 cut(s) 637, 665, 959, 1996, 4110, 4414
BveI ACCTGC 1 cut(s) 2077
Cac8I GCNNGC 2 cut(s) 3568, 4751
CaiI CAGNNNCTG 1 cut(s) 970
CciI TCATGA 3 cut(s) 2115, 3325, 4231
CfoI GCGC 3 cut(s) 3461, 3630, 4944
Cfr13I GGNCC 9 cut(s) 301, 1601, 2413, 2516, 3381, 3493, 3610, 3799, 4726
CseI GACGC 3 cut(s) 4229, 5001, 5068
CspCI CAANNNNNGTGG 2 cut(s) 4755, 4790
DraI TTTAAA 3 cut(s) 160, 1107, 2829
DraIII CACNNNGTG 1 cut(s) 2753
DriI GACNNNNNGTC 1 cut(s) 5144
Eam1104I CTCTTC 7 cut(s) 456, 3104, 3962, 4326, 4463, 4494, 5100
Eam1105I GACNNNNNGTC 1 cut(s) 5144
EarI CTCTTC 7 cut(s) 456, 3104, 3962, 4326, 4463, 4494, 5100
EciI GGCGGA 1 cut(s) 1152
Eco130I CCWWGG 6 cut(s) 826, 901, 3155, 3999, 4284, 4604
Eco147I AGGCCT 1 cut(s) 591
Eco31I GGTCTC 1 cut(s) 2812
Eco32I GATATC 2 cut(s) 1160, 2590
Eco47I GGWCC 6 cut(s) 301, 2413, 3381, 3493, 3799, 4726
Eco47III AGCGCT 1 cut(s) 4943
Eco57I CTGAAG 8 cut(s) 70, 71, 277, 2695, 2787, 3120, 3923, 3998
Eco72I CACGTG 2 cut(s) 2750, 3416
EcoO109I RGGNCCY 3 cut(s) 301, 3381, 4726
EcoRI GAATTC 2 cut(s) 1578, 2484
EcoRII CCWGG 6 cut(s) 285, 1716, 2331, 3606, 3668, 4730
EcoRV GATATC 2 cut(s) 1160, 2590
EcoT14I CCWWGG 6 cut(s) 826, 901, 3155, 3999, 4284, 4604
EcoT22I ATGCAT 1 cut(s) 1593
ErhI CCWWGG 6 cut(s) 826, 901, 3155, 3999, 4284, 4604
Esp3I CGTCTC 1 cut(s) 4487
FalI AAGNNNNNCTT 2 cut(s) 1902, 1934
FaqI GGGAC 5 cut(s) 287, 1570, 2577, 3114, 3394
FauNDI CATATG 1 cut(s) 2725
FbaI TGATCA 3 cut(s) 3220, 3328, 4588
FblI GTMKAC 2 cut(s) 2122, 4477
GlaI GCGC 3 cut(s) 3460, 3629, 4943
GsaI CCCAGC 2 cut(s) 2623, 4743
GsuI CTGGAG 3 cut(s) 3740, 3790, 4216
HaeII RGCGCY 1 cut(s) 4945
HaeIII GGCC 5 cut(s) 591, 1602, 2517, 3611, 4430
HapII CCGG 6 cut(s) 713, 2481, 4256, 4658, 4700, 5063
HgaI GACGC 3 cut(s) 4229, 5001, 5068
HhaI GCGC 3 cut(s) 3461, 3630, 4944
Hin1I GRCGYC 3 cut(s) 1877, 3103, 5060
Hin6I GCGC 3 cut(s) 3459, 3628, 4942
HinP1I GCGC 3 cut(s) 3459, 3628, 4942
HincII GTYRAC 2 cut(s) 3046, 4571
HindII GTYRAC 2 cut(s) 3046, 4571
HindIII AAGCTT 1 cut(s) 308
HpaI GTTAAC 1 cut(s) 3046
HpaII CCGG 6 cut(s) 713, 2481, 4256, 4658, 4700, 5063
HphI GGTGA 7 cut(s) 347, 959, 988, 1358, 2345, 3161, 3948
Hpy99I CGWCG 2 cut(s) 2660, 4995
HpyF10VI GCNNNNNNNGC 5 cut(s) 926, 1027, 1932, 3641, 4037
Hsp92I GRCGYC 3 cut(s) 1877, 3103, 5060
HspAI GCGC 3 cut(s) 3459, 3628, 4942
Kpn2I TCCGGA 1 cut(s) 4699
Ksp22I TGATCA 3 cut(s) 3220, 3328, 4588
KspAI GTTAAC 1 cut(s) 3046
LmnI GCTCC 5 cut(s) 559, 745, 926, 1741, 3715
MfeI CAATTG 5 cut(s) 1841, 2916, 3658, 3723, 3794
MflI RGATCY 9 cut(s) 253, 370, 446, 839, 881, 1081, 1293, 3002, 4735
MhlI GDGCHC 4 cut(s) 84, 783, 1691, 4716
MlyI GAGTC 6 cut(s) 976, 2509, 2932, 3788, 3925, 5030
MmeI TCCRAC 7 cut(s) 400, 1130, 1296, 1411, 2486, 4268, 5027
Mph1103I ATGCAT 1 cut(s) 1593
MroI TCCGGA 1 cut(s) 4699
MroXI GAANNNNTTC 2 cut(s) 1218, 3113
MslI CAYNNNNRTG 9 cut(s) 831, 1551, 2232, 2242, 2458, 3171, 3330, 3961, 4185
MspA1I CMGCKG 1 cut(s) 4743
MspCI CTTAAG 1 cut(s) 1322
MspI CCGG 6 cut(s) 713, 2481, 4256, 4658, 4700, 5063
MspR9I CCNGG 7 cut(s) 287, 1718, 2333, 3608, 3670, 4256, 4732
MunI CAATTG 5 cut(s) 1841, 2916, 3658, 3723, 3794
Mva1269I GAATGC 1 cut(s) 134
MvaI CCWGG 6 cut(s) 287, 1718, 2333, 3608, 3670, 4732
MwoI GCNNNNNNNGC 5 cut(s) 926, 1027, 1932, 3641, 4037
NciI CCSGG 1 cut(s) 4256
NcoI CCATGG 1 cut(s) 826
NdeI CATATG 1 cut(s) 2725
NlaIV GGNNCC 9 cut(s) 303, 623, 841, 1314, 1634, 3382, 4715, 4728, 4737
NmuCI GTSAC 6 cut(s) 684, 1536, 2069, 2524, 4271, 4778
NsiI ATGCAT 1 cut(s) 1593
NspI RCATGY 4 cut(s) 1471, 2231, 3410, 3587
NspV TTCGAA 1 cut(s) 454
OliI CACNNNNGTG 2 cut(s) 2458, 3961
PagI TCATGA 3 cut(s) 2115, 3325, 4231
PasI CCCWGGG 1 cut(s) 4731
PceI AGGCCT 1 cut(s) 591
PciI ACATGT 3 cut(s) 1467, 2227, 3406
PctI GAATGC 1 cut(s) 134
PdmI GAANNNNTTC 2 cut(s) 1218, 3113
PfeI GAWTC 8 cut(s) 785, 805, 2933, 3439, 4158, 4303, 4362, 4580
PflFI GACNNNGTC 2 cut(s) 2719, 5069
PflMI CCANNNNNTGG 5 cut(s) 382, 583, 1376, 1393, 2753
PleI GAGTC 6 cut(s) 975, 2508, 2931, 3787, 3925, 5029
PmaCI CACGTG 2 cut(s) 2750, 3416
PmlI CACGTG 2 cut(s) 2750, 3416
PpsI GAGTC 6 cut(s) 975, 2508, 2931, 3787, 3925, 5029
Ppu21I YACGTR 4 cut(s) 698, 2556, 2750, 3416
PpuMI RGGWCCY 3 cut(s) 301, 3381, 4726
PscI ACATGT 3 cut(s) 1467, 2227, 3406
PshAI GACNNNNGTC 1 cut(s) 4239
PshBI ATTAAT 1 cut(s) 3477
PsiI TTATAA 1 cut(s) 4862
Psp1406I AACGTT 1 cut(s) 3870
Psp5II RGGWCCY 3 cut(s) 301, 3381, 4726
Psp6I CCWGG 6 cut(s) 285, 1716, 2331, 3606, 3668, 4730
PspCI CACGTG 2 cut(s) 2750, 3416
PspFI CCCAGC 2 cut(s) 2619, 4739
PspGI CCWGG 6 cut(s) 285, 1716, 2331, 3606, 3668, 4730
PspN4I GGNNCC 9 cut(s) 303, 623, 841, 1314, 1634, 3382, 4715, 4728, 4737
PspPI GGNCC 9 cut(s) 301, 1601, 2413, 2516, 3381, 3493, 3610, 3799, 4726
PspPPI RGGWCCY 3 cut(s) 301, 3381, 4726
PsrI GAACNNNNNNTAC 2 cut(s) 3402, 3434
PstI CTGCAG 5 cut(s) 966, 1631, 1950, 2878, 3038
PstNI CAGNNNCTG 1 cut(s) 970
PsuI RGATCY 9 cut(s) 253, 370, 446, 839, 881, 1081, 1293, 3002, 4735
PsyI GACNNNGTC 2 cut(s) 2719, 5069
PvuII CAGCTG 1 cut(s) 4743
RseI CAYNNNNRTG 9 cut(s) 831, 1551, 2232, 2242, 2458, 3171, 3330, 3961, 4185
Sau96I GGNCC 9 cut(s) 301, 1601, 2413, 2516, 3381, 3493, 3610, 3799, 4726
ScaI AGTACT 1 cut(s) 3685
SchI GAGTC 6 cut(s) 976, 2509, 2932, 3788, 3925, 5030
ScrFI CCNGG 7 cut(s) 287, 1718, 2333, 3608, 3670, 4256, 4732
SduI GDGCHC 4 cut(s) 84, 783, 1691, 4716
SfcI CTRYAG 7 cut(s) 962, 1627, 1930, 1946, 2189, 2874, 3034
SfuI TTCGAA 1 cut(s) 454
SinI GGWCC 6 cut(s) 301, 2413, 3381, 3493, 3799, 4726
SmiI ATTTAAAT 1 cut(s) 1107
SmiMI CAYNNNNRTG 9 cut(s) 831, 1551, 2232, 2242, 2458, 3171, 3330, 3961, 4185
SmlI CTYRAG 7 cut(s) 311, 800, 1322, 2594, 3359, 4276, 4340
SmoI CTYRAG 7 cut(s) 311, 800, 1322, 2594, 3359, 4276, 4340
SpeI ACTAGT 1 cut(s) 4573
SseBI AGGCCT 1 cut(s) 591
SsiI CCGC 6 cut(s) 199, 1163, 2666, 3730, 4717, 5093
SspI AATATT 3 cut(s) 763, 4075, 4597
StuI AGGCCT 1 cut(s) 591
StyD4I CCNGG 7 cut(s) 285, 1716, 2331, 3606, 3668, 4254, 4730
StyI CCWWGG 6 cut(s) 826, 901, 3155, 3999, 4284, 4604
SwaI ATTTAAAT 1 cut(s) 1107
TaqII GACCGA 2 cut(s) 2057, 2430
TatI WGTACW 7 cut(s) 2465, 3266, 3683, 3694, 3913, 4488, 4949
TauI GCSGC 2 cut(s) 201, 3733
TfiI GAWTC 8 cut(s) 785, 805, 2933, 3439, 4158, 4303, 4362, 4580
TscAI CASTG 6 cut(s) 637, 672, 966, 1996, 4110, 4414
TseFI GTSAC 6 cut(s) 684, 1536, 2069, 2524, 4271, 4778
Tsp45I GTSAC 6 cut(s) 684, 1536, 2069, 2524, 4271, 4778
TspGWI ACGGA 7 cut(s) 1077, 1906, 2512, 3366, 4415, 4556, 5069
TspRI CASTG 6 cut(s) 637, 672, 966, 1996, 4110, 4414
Tth111I GACNNNGTC 2 cut(s) 2719, 5069
Van91I CCANNNNNTGG 5 cut(s) 382, 583, 1376, 1393, 2753
Vha464I CTTAAG 1 cut(s) 1322
VneI GTGCAC 1 cut(s) 80
VpaK11BI GGWCC 6 cut(s) 301, 2413, 3381, 3493, 3799, 4726
VspI ATTAAT 1 cut(s) 3477
XbaI TCTAGA 2 cut(s) 415, 1426
XceI RCATGY 4 cut(s) 1471, 2231, 3410, 3587
XcmI CCANNNNNNNNNTGG 1 cut(s) 833
XmaJI CCTAGG 1 cut(s) 901
XmiI GTMKAC 2 cut(s) 2122, 4477
XmnI GAANNNNTTC 2 cut(s) 1218, 3113
ZraI GACGTC 2 cut(s) 1878, 3104
ZrmI AGTACT 1 cut(s) 3685
Zsp2I ATGCAT 1 cut(s) 1593
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.