Rh5AG280400

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000307
Physical Location & Seq
Reverse (-)
37952540 .. 37956293
3754 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG280400.1

Sequence Viewer

Length: 3294 bp
ATGTTGATCAGCAAACCTGAAATCATCTCCGATGGATATTTGCAGGATCTTGATACCTGGAGCACTCTGAATTGGAGTTGCAGTTTCCGTGAAACAGATTTAAGAAATAATTTTCATTTGATGCGTTGTGACAATTCTCCTCAGATGTTCTTTGATTCCTGTCAATATTCATCTTTGACATCTTTACTTCTGAGTGGATGCAAGCTAGGCGAAGGAGCAATCCCTGATGATATTGGCTGCTTATACTCACTCCATTTGTTAGACTTGAGTGAAAACAATTTCGTGAGCATACCTGAAACCATTTCTCAACTTTCCAAGCTCAGAAAGCTTCTGTTGTTTAGGTGCAGCAAACTTCAGTATTTGCCAAAACAACTTCCACTGAGTTTAAAACGTGTAATTGCACGCCATTGTCCGATGCTGAGAACTACTTATCCAAATACTATTGTAAAATGGACTTCAGATGAAGGTTTCAGTTTCATAGATTGCAGATATTCAGACGAAAATGCTGACGATGATGAAGGATATATGAATTTCCATAAGATCATTGAGGATCGAATTCATCATGACAAGCCATTTGAAATTCGTTTTGGTGATAGTCGTGCTGACTTTTCTAATGTCTGGTGCACTCAGTGGAACACTGGTTCTTCTGTTACACTGCAACTAACTGGTCCAGAAGACAAGACTAATAGTACGTGGATGGGATTAGCCTTATTTGTTGCTTTTGAACTCATTCCGAATGGTCAAGTGAAAGAAACTCTCTGTCATCTGAGCATCGACGAAGGTGGTCCCCAAATTCGTTTAGTTTTTGAAGACTTGGATTATGAGTCACTTGGACTCTGTTGCTACTTGCCACGAGGCTGCGGGGGTTTTGCAGGACAGCTGGATAAAGCCAGTCGAATTAATGCTACATTTTTGCCAACTACTTTACCGGTGAATGCGTGTGGGATACATCCAATATCCGAGCATGACGCTCCAATGTTTGCTCAGGATTTAGCCCGGGCTGCTTTTGCTGACGCACGTGCTTTTGCTGAACAGGAAAAATTGTTAACTCCTTTTGCTGTCGCACGTGCTGTTGCTAAACAGGAAAAATTGTTAAATTACGCTGATGGGAAGGCACGTGCTGATTGGAAGGCACTCATTTGTCGACATGGTAAGAGAATGTTAGATAAGGCAACGAAATTGGAAAGATCGGGAGACGTGCTGGTACCTGCGTACAATTCTTTAAATGTGAATCGACACAAGTGGAAAATTGATCCTGATATCCATCCCAGATTAGAGGAAGAGCTAAAGCTGTTCAGATTGCTTTCAGGGCTCTACGAGGGAAGCAATGGACGTCAGAAGAAATTTCATTTTTGTTTTCCAGATCAAGTAATTTCGCCCTGGTTCATCAATCACTACGCAGGGAATGTAGCACTTTGTGACCTTCCTCAGAACTTAATCGATGATAAGAGATGGATTGGATTAGAAGTGTATGTTGTGTTTACACCTGGTTACAGTGACCACAATCTTTCTGATTCCTTCCTTCATGTTGATTTAATGGCACATGAAAGTAGTGTAATGCATTCGTCCATCAAAATTGAGAGTTGTTCTGGGTCATGTCAACTTATTGTACTACATGTACCACGGATGCATTTTCCGGAACAGTTGACTCATTATCAGGTTATTAGCACGTTGTTTACAACCAGTACCCCAATTGTTGAGATTATAGTGTGCGGGAGCCGTTTGGTATATGAGCATGATCTGGAAGATTTAATCCTTTCATTAACTGCGTGTACATTGAGGAAGCCATATTCAATTGGCCAAGTTTATTCCCAGGCCCAAACATATGTTGAAACTACGGAAAAGAAAGCAGATGAAAATATCCAGTGCAATGCAAAGCGAGGAGAAATGTCATCCACTGATGGCCATTCATCGTTTCAAAGAAGTGTCAAACAAATTAAAGTACCAGATAAAGTTCGCAGTTCAATATTGCAGAAATATAGATGCCATATCCAAGGAAAGCAAGGTATACTAGAGTCTGACAAAATAATTGTCCACTCTAAATCCCTTCTTCTGGACAATAGCATCAAAGAAAATTTTACTGGAAAAGAATCAATAATGTCGTCGTTGGCATGTCATCATTTGCATATAGTGGCTGAGACTCACTTCTGGGGAAGCTATAGTGCCCAACAGTGGAAGAGATGCCTTGATTTGTTGCTGAAATATTCTGAGGTGGCAACTCTCAGTCTTAATGGACATGTAATCTCTGTAATGAAGCATTTTAGTCCCTCCTCGCCATTCAATTTTTGTTTCCCTAAAGATGAAGTTCTGCTAGACTGGATGAGAGATCGTCGACTCTATGATGATAAAAGTTTCAGGGGACTTGTTATATGTGCAACTTTTTCGTTTGACGATGATTCTGCCATGAACAATCTGGCTTCAGAAATTACTGTTGAACTCTTGTGTCACTTGAGTTACAATGGCTCCAGGTTTTGCGTGAACGCTGCCCCCATGCTTACGATCACTAAGGAGAAATTCCAGTGGCTTTATTTCCGTGATTTCATTTGGGTTACGTATGTACCCCTTGTTATGCTAGCGGAGGTCAAAGCAATCAGTGATATAGAGGCTGATATATGTAGTAATTGCCCAGGCTTGAGGGTGGACTGCACTTATACATGTGTCTTGCGCCGGCAAGAGGAGGAGGAATTTAAGCAAAAAATAACTCAGTGCTGGACCTCCTTCTTTGATAATATCGATCTCCTCCGTCGGTTTGTACAAGCAGATGCTGAAAAAGGTGTAGAGACGAAGCACGAACTTCCAATGCTCGGCGGAAGTATGAAGGACCTTGATAGCAACTTGATGTATGACGCAACACACCCATTTACTGAAATTCCGGATTGGTTTGGACATCCAAATGAAAGGTCTGTAGAGATCCAGTTACCACCATCTCTTGTCAGGGACGAAAATTGGATAGGAGTGGCTATATGTGCATCATATTTTCCCGTACAATTGAATCCTCAAGTTTCTGCGTACTTAAAATGTGTTTTGCGAACTGAGAAGAACAACTTGAAATCTGTCCACCGCTATCAGATGACCAATGAAGAAGTCAAGTGCGTTTCTTCTGAGAGAAAATTCATTTGGCTATCCTATCTTCCACGACACTGGTTTTTCTACCAGTTAAATGATGAGTCTGCTCTCAGGGCTTCTTCGTTTTCAAGTTCACCTGGTTTGTGGTTTAAGGAGTGCGGTCTGCGTCTTGTATACCGGCAAGATGAGGAAGAGTTTAAGCAACTCTGTATCTCCTTAAACTCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

1097

Amino Acids

125.56

Weight (kDa)

5.98

Isoelectric Point (pI)

45.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C-JID PF20160 953 - 1086 1.5e-09 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000307)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g16600 FvH4_1g16610 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16621 FvH4_1g16630 FvH4_1g16640 FvH4_1g16640 FvH4_1g16650 FvH4_1g16650 FvH4_3g27631 FvH4_4g11392 FvH4_4g11542
prunus_persica Prupe.4G224500_v2.0.a1 Prupe.4G226900_v2.0.a1 Prupe.4G227000_v2.0.a1
pyrus_communis pycom11g18180 pycom11g18210 pycom11g18230 pycom11g18270 pycom11g18350 pycom11g18360 pycom11g18370
rosa_chinensis RchiOBHm_Chr2g0106511 RchiOBHm_Chr2g0106541 RchiOBHm_Chr2g0106551 RchiOBHm_Chr2g0106571 RchiOBHm_Chr2g0124621 RchiOBHm_Chr6g0251591 RchiOBHm_Chr6g0251631 RchiOBHm_Chr6g0251641
rosa_laevigata RLG00000015443 RLG00000015444 RLG00000015458 RLG00000017510 RLG00000017511 RLG00000017512 RLG00000017515 RLG00000017516 RLG00000017517 RLG00000018809 RLG00000020934 RLG00000020935 RLG00000034092
rosa_multiflora Rmu_co8285777.1_g000001 Rmu_sc0000762.1_g000001 Rmu_sc0001872.1_g000006 Rmu_sc0005255.1_g000019 Rmu_sc0005597.1_g000006 Rmu_sc0012104.1_g000001 Rmu_sc0017105.1_g000011 Rmu_sc0022904.1_g000001 Rmu_ssc0000135.1_g000049 Rmu_ssc0000213.1_g000047
rosa_roxburghii Rroxscaffold_2G00092830 Rroxscaffold_2G00119790 Rroxscaffold_2G00137010 Rroxscaffold_2G00137030 Rroxscaffold_2G00137070 Rroxscaffold_7G00216560
rosa_rugosa Rorug02G0136100 Rorug02G0136200 Rorug02G0136300 Rorug02G0136400 Rorug02G0251800 Rorug02G0251800 Rorug02G0455500 Rorug02G0455600 Rorug02G0455700 Rorug05G0195300
rosa_samantha Rh2AG187400 Rh2AG187500 Rh2AG187600 Rh2AG310500 Rh2BG198200 Rh2BG198300 Rh2BG198400 Rh2BG198600 Rh2BG318900 Rh2BG534300 Rh2CG192400 Rh2CG192500 Rh2CG192600 Rh2CG297500 Rh2DG193500 Rh2DG193600 Rh2DG193800 Rh2DG334200 Rh2DG543100 Rh5AG280400 Rh5BG285800 Rh5BG285900 Rh5CG317900 Rh5CG318000 Rh5DG294600 Rh5DG294700 Rh6AG012200 Rh6AG012700 Rh6BG011200 Rh6BG011700
rosa_wichuraiana Rw0G009380 Rw2G014610 Rw2G014630 Rw2G014640 Rw2G014680 Rw2G025120 Rw2G043000 Rw5G026490 Rw6G001370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1336
Acc36I ACCTGC 1 cut(s) 1216
Acc65I GGTACC 1 cut(s) 1204
AccB1I GGYRCC 1 cut(s) 1204
AccI GTMKAC 4 cut(s) 1144, 2008, 2332, 3240
AccIII TCCGGA 2 cut(s) 1636, 2872
AciI CCGC 6 cut(s) 861, 1713, 2576, 2808, 3061, 3225
AclWI GGATC 4 cut(s) 54, 558, 1247, 2905
AcoI YGGCCR 2 cut(s) 1798, 1903
AcuI CTGAAG 3 cut(s) 338, 441, 2403
AcvI CACGTG 3 cut(s) 1019, 1067, 1118
AcyI GRCGYC 1 cut(s) 1333
AdeI CACNNNGTG 2 cut(s) 630, 1418
AfiI CCNNNNNNNGG 4 cut(s) 2053, 2172, 2674, 2804
AflIII ACRYGT 4 cut(s) 391, 1615, 2236, 2654
AgeI ACCGGT 1 cut(s) 928
AjiI CACGTC 1 cut(s) 1198
AjnI CCWGG 7 cut(s) 56, 1379, 1486, 1812, 2465, 2626, 3202
AjuI GAANNNNNNNTTGG 4 cut(s) 570, 602, 3100, 3132
AloI GAACNNNNNNTCC 4 cut(s) 415, 447, 625, 657
AluBI AGCT 7 cut(s) 205, 319, 328, 880, 1285, 1291, 2157
AluI AGCT 7 cut(s) 205, 319, 328, 880, 1285, 1291, 2157
Alw21I GWGCWC 2 cut(s) 65, 626
Alw26I GTCTC 3 cut(s) 1188, 2132, 2774
Alw44I GTGCAC 1 cut(s) 622
AlwI GGATC 4 cut(s) 54, 558, 1247, 2905
AlwNI CAGNNNCTG 1 cut(s) 2765
Ama87I CYCGRG 1 cut(s) 996
Aor13HI TCCGGA 2 cut(s) 1636, 2872
AoxI GGCC 3 cut(s) 1798, 1815, 1903
ApaLI GTGCAC 1 cut(s) 622
ApeKI GCWGC 5 cut(s) 237, 345, 858, 1001, 2483
ArsI GACNNNNNNTTYG 6 cut(s) 557, 589, 2016, 2048, 2885, 2917
AseI ATTAAT 1 cut(s) 900
AsiGI ACCGGT 1 cut(s) 928
Asp700I GAANNNNTTC 1 cut(s) 729
Asp718I GGTACC 1 cut(s) 1204
AspLEI GCGC 1 cut(s) 2667
AspS9I GGNCC 5 cut(s) 668, 785, 1816, 2712, 2821
AsuC2I CCSGG 2 cut(s) 997, 998
AsuHPI GGTGA 3 cut(s) 602, 943, 3192
AsuNHI GCTAGC 1 cut(s) 2572
AvaI CYCGRG 1 cut(s) 996
AvaII GGWCC 4 cut(s) 668, 785, 2712, 2821
BaeGI GKGCMC 2 cut(s) 626, 2167
BaeI ACNNNNGTAYC 4 cut(s) 1196, 1229, 2541, 2574
BalI TGGCCA 2 cut(s) 1800, 1905
BanI GGYRCC 1 cut(s) 1204
BanII GRGCYC 1 cut(s) 1314
BauI CACGAG 1 cut(s) 852
BbrPI CACGTG 3 cut(s) 1019, 1067, 1118
BbsI GAAGAC 2 cut(s) 681, 816
Bbv12I GWGCWC 2 cut(s) 65, 626
BbvI GCAGC 5 cut(s) 224, 357, 845, 988, 2470
BccI CCATC 8 cut(s) 26, 691, 1100, 1272, 1446, 1577, 1895, 2932
BceAI ACGGC 1 cut(s) 1704
BcgI CGANNNNNNTGC 4 cut(s) 2364, 2381, 2398, 2415
BciT130I CCWGG 7 cut(s) 58, 1381, 1488, 1814, 2467, 2628, 3204
BciVI GTATCC 1 cut(s) 939
BclI TGATCA 1 cut(s) 6
BcnI CCSGG 2 cut(s) 997, 998
BcoDI GTCTC 3 cut(s) 1188, 2132, 2774
BfaI CTAG 4 cut(s) 206, 2012, 2312, 2573
BfmI CTRYAG 2 cut(s) 2158, 2904
BfuAI ACCTGC 1 cut(s) 1216
BfuI GTATCC 1 cut(s) 939
BisI GCNGC 5 cut(s) 238, 346, 859, 1002, 2484
BlsI GCNGC 5 cut(s) 239, 347, 860, 1003, 2485
Bme1390I CCNGG 9 cut(s) 58, 997, 998, 1381, 1488, 1814, 2467, 2628, 3204
Bme18I GGWCC 4 cut(s) 668, 785, 2712, 2821
BmeT110I CYCGRG 1 cut(s) 996
BmgBI CACGTC 1 cut(s) 1198
BmgT120I GGNCC 5 cut(s) 668, 785, 1816, 2712, 2821
BmiI GGNNCC 4 cut(s) 787, 1206, 1718, 2464
BmrFI CCNGG 9 cut(s) 58, 997, 998, 1381, 1488, 1814, 2467, 2628, 3204
BmtI GCTAGC 1 cut(s) 2576
BpiI GAAGAC 2 cut(s) 681, 816
BpmI CTGGAG 2 cut(s) 79, 2449
Bpu10I CCTNAGC 1 cut(s) 984
BpuEI CTTGAG 4 cut(s) 286, 2470, 2653, 2982
BpuMI CCSGG 2 cut(s) 997, 998
Bsa29I ATCGAT 2 cut(s) 1440, 2733
BsaAI YACGTR 5 cut(s) 693, 1019, 1067, 1118, 2553
BsaBI GATNNNNATC 1 cut(s) 1263
BsaHI GRCGYC 1 cut(s) 1333
BsaJI CCNNGG 6 cut(s) 996, 1379, 1622, 1812, 1993, 2626
BsaWI WCCGGW 3 cut(s) 928, 1636, 2872
BsaXI ACNNNNNCTCC 4 cut(s) 67, 97, 2447, 2477
Bsc4I CCNNNNNNNGG 4 cut(s) 2053, 2172, 2674, 2804
Bse118I RCCGGY 3 cut(s) 928, 2667, 3243
Bse3DI GCAATG 2 cut(s) 1333, 1876
Bse8I GATNNNNATC 1 cut(s) 1263
BseAI TCCGGA 2 cut(s) 1636, 2872
BseBI CCWGG 7 cut(s) 58, 1381, 1488, 1814, 2467, 2628, 3204
BseCI ATCGAT 2 cut(s) 1440, 2733
BseDI CCNNGG 6 cut(s) 996, 1379, 1622, 1812, 1993, 2626
BseGI GGATG 8 cut(s) 203, 702, 949, 1264, 1632, 1892, 2325, 2887
BseJI GATNNNNATC 1 cut(s) 1263
BseLI CCNNNNNNNGG 4 cut(s) 2053, 2172, 2674, 2804
BseMI GCAATG 2 cut(s) 1333, 1876
BseRI GAGGAG 6 cut(s) 129, 1896, 2260, 2690, 2693, 2729
BseSI GKGCMC 2 cut(s) 626, 2167
BseXI GCAGC 5 cut(s) 224, 357, 845, 988, 2470
BsgI GTGCAG 2 cut(s) 364, 2629
BshFI GGCC 3 cut(s) 1800, 1817, 1905
BshNI GGYRCC 1 cut(s) 1204
BshTI ACCGGT 1 cut(s) 928
BshVI ATCGAT 2 cut(s) 1440, 2733
BsiHKAI GWGCWC 2 cut(s) 65, 626
BsiHKCI CYCGRG 1 cut(s) 996
BsiSI CCGG 6 cut(s) 929, 997, 1637, 2668, 2873, 3244
BslFI GGGAC 4 cut(s) 771, 2250, 2373, 2951
BslI CCNNNNNNNGG 4 cut(s) 2053, 2172, 2674, 2804
BsmAI GTCTC 3 cut(s) 1188, 2132, 2774
BsmBI CGTCTC 2 cut(s) 1188, 2774
BsmFI GGGAC 4 cut(s) 771, 2250, 2373, 2951
BsmI GAATGC 2 cut(s) 940, 1561
BsnI GGCC 3 cut(s) 1800, 1817, 1905
BsoBI CYCGRG 1 cut(s) 996
Bsp1286I GDGCHC 4 cut(s) 65, 626, 1314, 2167
Bsp13I TCCGGA 2 cut(s) 1636, 2872
Bsp1407I TGTACA 2 cut(s) 1772, 2752
BspACI CCGC 6 cut(s) 861, 1713, 2576, 2808, 3061, 3225
BspANI GGCC 3 cut(s) 1800, 1817, 1905
BspDI ATCGAT 2 cut(s) 1440, 2733
BspEI TCCGGA 2 cut(s) 1636, 2872
BspHI TCATGA 1 cut(s) 562
BspLI GGNNCC 4 cut(s) 787, 1206, 1718, 2464
BspMI ACCTGC 1 cut(s) 1216
BspOI GCTAGC 1 cut(s) 2576
BspPI GGATC 4 cut(s) 54, 558, 1247, 2905
BspQI GCTCTTC 1 cut(s) 1275
BspT107I GGYRCC 1 cut(s) 1204
BsrDI GCAATG 2 cut(s) 1333, 1876
BsrFI RCCGGY 3 cut(s) 928, 2667, 3243
BsrGI TGTACA 2 cut(s) 1772, 2752
BssAI RCCGGY 3 cut(s) 928, 2667, 3243
BssECI CCNNGG 6 cut(s) 996, 1379, 1622, 1812, 1993, 2626
BssNAI GTATAC 2 cut(s) 2009, 3241
BssNI GRCGYC 1 cut(s) 1333
BssSI CACGAG 1 cut(s) 852
BssT1I CCWWGG 1 cut(s) 1993
Bst1107I GTATAC 2 cut(s) 2009, 3241
Bst2BI CACGAG 1 cut(s) 852
Bst2UI CCWGG 7 cut(s) 58, 1381, 1488, 1814, 2467, 2628, 3204
Bst4CI ACNGT 4 cut(s) 1496, 1644, 2172, 2431
Bst6I CTCTTC 3 cut(s) 1275, 2171, 3252
BstACI GRCGYC 1 cut(s) 1333
BstAUI TGTACA 2 cut(s) 1772, 2752
BstBAI YACGTR 5 cut(s) 693, 1019, 1067, 1118, 2553
BstC8I GCNNGC 4 cut(s) 203, 403, 2574, 2669
BstDSI CCRYGG 1 cut(s) 1622
BstF5I GGATG 8 cut(s) 203, 702, 949, 1264, 1632, 1892, 2325, 2887
BstHHI GCGC 1 cut(s) 2667
BstMAI GTCTC 3 cut(s) 1188, 2132, 2774
BstMWI GCNNNNNNNGC 7 cut(s) 207, 325, 1001, 1007, 1309, 2966, 3179
BstNI CCWGG 7 cut(s) 58, 1381, 1488, 1814, 2467, 2628, 3204
BstNSI RCATGY 4 cut(s) 1619, 2115, 2240, 2658
BstSCI CCNGG 9 cut(s) 56, 995, 996, 1379, 1486, 1812, 2465, 2626, 3202
BstSFI CTRYAG 2 cut(s) 2158, 2904
BstSLI GKGCMC 2 cut(s) 626, 2167
BstSNI TACGTA 1 cut(s) 2553
BstV1I GCAGC 5 cut(s) 224, 357, 845, 988, 2470
BstV2I GAAGAC 2 cut(s) 681, 816
BstX2I RGATCY 2 cut(s) 46, 2910
BstXI CCANNNNNNTGG 1 cut(s) 3141
BstYI RGATCY 2 cut(s) 46, 2910
BstZ17I GTATAC 2 cut(s) 2009, 3241
Bsu15I ATCGAT 2 cut(s) 1440, 2733
BsuI GTATCC 1 cut(s) 939
BsuRI GGCC 3 cut(s) 1800, 1817, 1905
BsuTUI ATCGAT 2 cut(s) 1440, 2733
BtgI CCRYGG 1 cut(s) 1622
BtrI CACGTC 1 cut(s) 1198
BtsCI GGATG 8 cut(s) 203, 702, 949, 1264, 1632, 1892, 2325, 2887
BtsI GCAGTG 1 cut(s) 653
BveI ACCTGC 1 cut(s) 1216
Cac8I GCNNGC 4 cut(s) 203, 403, 2574, 2669
CaiI CAGNNNCTG 1 cut(s) 2765
CciI TCATGA 1 cut(s) 562
CfoI GCGC 1 cut(s) 2667
Cfr10I RCCGGY 3 cut(s) 928, 2667, 3243
Cfr13I GGNCC 5 cut(s) 668, 785, 1816, 2712, 2821
Cfr9I CCCGGG 1 cut(s) 996
ClaI ATCGAT 2 cut(s) 1440, 2733
CseI GACGC 4 cut(s) 977, 1022, 2855, 3221
CsiI ACCWGGT 2 cut(s) 1486, 3202
CspAI ACCGGT 1 cut(s) 928
DraI TTTAAA 2 cut(s) 387, 1224
DraIII CACNNNGTG 2 cut(s) 630, 1418
EaeI YGGCCR 2 cut(s) 1798, 1903
Eam1104I CTCTTC 3 cut(s) 1275, 2171, 3252
EarI CTCTTC 3 cut(s) 1275, 2171, 3252
EciI GGCGGA 1 cut(s) 2823
Eco105I TACGTA 1 cut(s) 2553
Eco130I CCWWGG 1 cut(s) 1993
Eco24I GRGCYC 1 cut(s) 1314
Eco32I GATATC 1 cut(s) 1261
Eco47I GGWCC 4 cut(s) 668, 785, 2712, 2821
Eco57I CTGAAG 3 cut(s) 338, 441, 2403
Eco72I CACGTG 3 cut(s) 1019, 1067, 1118
Eco88I CYCGRG 1 cut(s) 996
EcoO109I RGGNCCY 1 cut(s) 2821
EcoRI GAATTC 1 cut(s) 555
EcoRII CCWGG 7 cut(s) 56, 1379, 1486, 1812, 2465, 2626, 3202
EcoRV GATATC 1 cut(s) 1261
EcoT14I CCWWGG 1 cut(s) 1993
EcoT22I ATGCAT 2 cut(s) 1563, 1632
EcoT38I GRGCYC 1 cut(s) 1314
ErhI CCWWGG 1 cut(s) 1993
Esp3I CGTCTC 2 cut(s) 1188, 2774
FalI AAGNNNNNCTT 2 cut(s) 3029, 3061
FaqI GGGAC 4 cut(s) 771, 2250, 2373, 2951
FauI CCCGC 2 cut(s) 854, 1706
FauNDI CATATG 1 cut(s) 1825
FbaI TGATCA 1 cut(s) 6
FblI GTMKAC 4 cut(s) 1144, 2008, 2332, 3240
Fnu4HI GCNGC 5 cut(s) 238, 346, 859, 1002, 2484
FokI GGATG 8 cut(s) 210, 709, 936, 1251, 1639, 1879, 2332, 2874
FriOI GRGCYC 1 cut(s) 1314
Fsp4HI GCNGC 5 cut(s) 238, 346, 859, 1002, 2484
FspBI CTAG 4 cut(s) 206, 2012, 2312, 2573
GlaI GCGC 1 cut(s) 2666
GluI GCNGC 5 cut(s) 238, 346, 859, 1002, 2484
GsuI CTGGAG 2 cut(s) 79, 2449
HaeIII GGCC 3 cut(s) 1800, 1817, 1905
HapII CCGG 6 cut(s) 929, 997, 1637, 2668, 2873, 3244
HgaI GACGC 4 cut(s) 977, 1022, 2855, 3221
HhaI GCGC 1 cut(s) 2667
Hin1I GRCGYC 1 cut(s) 1333
Hin6I GCGC 1 cut(s) 2665
HinP1I GCGC 1 cut(s) 2665
HincII GTYRAC 5 cut(s) 1047, 1145, 1601, 1647, 2333
HindII GTYRAC 5 cut(s) 1047, 1145, 1601, 1647, 2333
HindIII AAGCTT 1 cut(s) 326
HpaI GTTAAC 1 cut(s) 1047
HpaII CCGG 6 cut(s) 929, 997, 1637, 2668, 2873, 3244
HphI GGTGA 3 cut(s) 602, 943, 3192
Hpy99I CGWCG 4 cut(s) 779, 2107, 2334, 2748
HpyCH4III ACNGT 4 cut(s) 1496, 1644, 2172, 2431
HpyCH4IV ACGT 9 cut(s) 391, 692, 1018, 1066, 1117, 1197, 1333, 1670, 2552
HpyF10VI GCNNNNNNNGC 7 cut(s) 207, 325, 1001, 1007, 1309, 2966, 3179
HpySE526I ACGT 9 cut(s) 391, 692, 1018, 1066, 1117, 1197, 1333, 1670, 2552
Hsp92I GRCGYC 1 cut(s) 1333
HspAI GCGC 1 cut(s) 2665
Kpn2I TCCGGA 2 cut(s) 1636, 2872
KpnI GGTACC 1 cut(s) 1208
KroI GCCGGC 1 cut(s) 2667
KroNI GCCGGC 1 cut(s) 2669
Ksp22I TGATCA 1 cut(s) 6
KspAI GTTAAC 1 cut(s) 1047
LguI GCTCTTC 1 cut(s) 1275
LmnI GCTCC 5 cut(s) 60, 215, 976, 1716, 2468
Lsp1109I GCAGC 5 cut(s) 224, 357, 845, 988, 2470
MabI ACCWGGT 2 cut(s) 1486, 3202
MaeI CTAG 4 cut(s) 206, 2012, 2312, 2573
MaeII ACGT 9 cut(s) 391, 692, 1018, 1066, 1117, 1197, 1333, 1670, 2552
MfeI CAATTG 3 cut(s) 1692, 1794, 2987
MflI RGATCY 2 cut(s) 46, 2910
MhlI GDGCHC 4 cut(s) 65, 626, 1314, 2167
MlsI TGGCCA 2 cut(s) 1800, 1905
MluNI TGGCCA 2 cut(s) 1800, 1905
MlyI GAGTC 7 cut(s) 828, 833, 1642, 2024, 2134, 2328, 3176
Mox20I TGGCCA 2 cut(s) 1800, 1905
Mph1103I ATGCAT 2 cut(s) 1563, 1632
MroI TCCGGA 2 cut(s) 1636, 2872
MroNI GCCGGC 1 cut(s) 2667
MroXI GAANNNNTTC 1 cut(s) 729
MscI TGGCCA 2 cut(s) 1800, 1905
MslI CAYNNNNRTG 1 cut(s) 2892
Msp20I TGGCCA 2 cut(s) 1800, 1905
MspA1I CMGCKG 1 cut(s) 880
MspI CCGG 6 cut(s) 929, 997, 1637, 2668, 2873, 3244
MspR9I CCNGG 9 cut(s) 58, 997, 998, 1381, 1488, 1814, 2467, 2628, 3204
MunI CAATTG 3 cut(s) 1692, 1794, 2987
Mva1269I GAATGC 2 cut(s) 940, 1561
MvaI CCWGG 7 cut(s) 58, 1381, 1488, 1814, 2467, 2628, 3204
MwoI GCNNNNNNNGC 7 cut(s) 207, 325, 1001, 1007, 1309, 2966, 3179
NaeI GCCGGC 1 cut(s) 2669
NciI CCSGG 2 cut(s) 997, 998
NdeI CATATG 1 cut(s) 1825
NgoMIV GCCGGC 1 cut(s) 2667
NheI GCTAGC 1 cut(s) 2572
NlaIV GGNNCC 4 cut(s) 787, 1206, 1718, 2464
NmeAIII GCCGAG 1 cut(s) 2784
NmuCI GTSAC 5 cut(s) 128, 825, 1418, 1496, 2444
NsiI ATGCAT 2 cut(s) 1563, 1632
NspI RCATGY 4 cut(s) 1619, 2115, 2240, 2658
PagI TCATGA 1 cut(s) 562
PciI ACATGT 3 cut(s) 1615, 2236, 2654
PciSI GCTCTTC 1 cut(s) 1275
PctI GAATGC 2 cut(s) 940, 1561
PdiI GCCGGC 1 cut(s) 2669
PdmI GAANNNNTTC 1 cut(s) 729
PfeI GAWTC 6 cut(s) 155, 1231, 1514, 2090, 2396, 2992
PinAI ACCGGT 1 cut(s) 928
PkrI GCNGC 5 cut(s) 239, 347, 860, 1003, 2485
PleI GAGTC 7 cut(s) 828, 832, 1642, 2023, 2134, 2328, 3175
PmaCI CACGTG 3 cut(s) 1019, 1067, 1118
PmlI CACGTG 3 cut(s) 1019, 1067, 1118
PpsI GAGTC 7 cut(s) 828, 832, 1642, 2023, 2134, 2328, 3175
Ppu21I YACGTR 5 cut(s) 693, 1019, 1067, 1118, 2553
PpuMI RGGWCCY 1 cut(s) 2821
PscI ACATGT 3 cut(s) 1615, 2236, 2654
PshBI ATTAAT 1 cut(s) 900
Psp5II RGGWCCY 1 cut(s) 2821
Psp6I CCWGG 7 cut(s) 56, 1379, 1486, 1812, 2465, 2626, 3202
PspCI CACGTG 3 cut(s) 1019, 1067, 1118
PspGI CCWGG 7 cut(s) 56, 1379, 1486, 1812, 2465, 2626, 3202
PspN4I GGNNCC 4 cut(s) 787, 1206, 1718, 2464
PspPI GGNCC 5 cut(s) 668, 785, 1816, 2712, 2821
PspPPI RGGWCCY 1 cut(s) 2821
PstNI CAGNNNCTG 1 cut(s) 2765
PsuI RGATCY 2 cut(s) 46, 2910
PvuII CAGCTG 1 cut(s) 880
RseI CAYNNNNRTG 1 cut(s) 2892
SalI GTCGAC 2 cut(s) 1143, 2331
SapI GCTCTTC 1 cut(s) 1275
SatI GCNGC 5 cut(s) 238, 346, 859, 1002, 2484
Sau96I GGNCC 5 cut(s) 668, 785, 1816, 2712, 2821
SchI GAGTC 7 cut(s) 828, 833, 1642, 2024, 2134, 2328, 3176
ScrFI CCNGG 9 cut(s) 58, 997, 998, 1381, 1488, 1814, 2467, 2628, 3204
SduI GDGCHC 4 cut(s) 65, 626, 1314, 2167
SexAI ACCWGGT 2 cut(s) 1486, 3202
SfcI CTRYAG 2 cut(s) 2158, 2904
SinI GGWCC 4 cut(s) 668, 785, 2712, 2821
SmaI CCCGGG 1 cut(s) 998
SmiMI CAYNNNNRTG 1 cut(s) 2892
SmlI CTYRAG 4 cut(s) 265, 2449, 2632, 2997
SmoI CTYRAG 4 cut(s) 265, 2449, 2632, 2997
SnaBI TACGTA 1 cut(s) 2553
SrfI GCCCGGGC 1 cut(s) 998
SsiI CCGC 6 cut(s) 861, 1713, 2576, 2808, 3061, 3225
SspI AATATT 3 cut(s) 167, 1968, 2204
SspMI CTAG 4 cut(s) 206, 2012, 2312, 2573
StyD4I CCNGG 9 cut(s) 56, 995, 996, 1379, 1486, 1812, 2465, 2626, 3202
StyI CCWWGG 1 cut(s) 1993
TaaI ACNGT 4 cut(s) 1496, 1644, 2172, 2431
TaiI ACGT 9 cut(s) 394, 695, 1021, 1069, 1120, 1200, 1336, 1673, 2555
TaqI TCGA 8 cut(s) 553, 774, 895, 1144, 1234, 1440, 2332, 2733
TatI WGTACW 3 cut(s) 1609, 1772, 2752
TfiI GAWTC 6 cut(s) 155, 1231, 1514, 2090, 2396, 2992
TseFI GTSAC 5 cut(s) 128, 825, 1418, 1496, 2444
TseI GCWGC 5 cut(s) 237, 345, 858, 1001, 2483
Tsp45I GTSAC 5 cut(s) 128, 825, 1418, 1496, 2444
TspGWI ACGGA 5 cut(s) 77, 1639, 1853, 2522, 2732
TspMI CCCGGG 1 cut(s) 996
VneI GTGCAC 1 cut(s) 622
VpaK11BI GGWCC 4 cut(s) 668, 785, 2712, 2821
VspI ATTAAT 1 cut(s) 900
XceI RCATGY 4 cut(s) 1619, 2115, 2240, 2658
XcmI CCANNNNNNNNNTGG 1 cut(s) 2410
XmaI CCCGGG 1 cut(s) 996
XmiI GTMKAC 4 cut(s) 1144, 2008, 2332, 3240
XmnI GAANNNNTTC 1 cut(s) 729
XspI CTAG 4 cut(s) 206, 2012, 2312, 2573
ZraI GACGTC 1 cut(s) 1334
Zsp2I ATGCAT 2 cut(s) 1563, 1632
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.