pycom11g18270

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0000307
Physical Location & Seq
Forward (+)
20455285 .. 20469391
14107 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g18270.9

Sequence Viewer

Length: 5748 bp
ATGTGGTTAATTTTAAATAAAATATTTAAAATAATTTCTAACTATTCGGTGAGAATCCGAATTCTTTATTTTCAACTTCTTTTGGTTGCCACTTACAATCTATTATGTATTATCTTGTTAAAAATAAATTTGAATTATAACCTATTACGAAGCTTAACACATTTCTCTCTCCTTCATTTGTTAAAAAAAAAAAATAATAATAATAATATTTTTTTGCTCCATGTCCATCTCTCAACCCCAAGATTGAGTATCATCTCCGTCGTCTCAATGCGCTCTCTCTCCCACTCTATCTGCTTCTCTCCAACATCCTCCTCTCATACGAACTCCAATATCGACGCTGCCCACCCCTCTCGACCTGGAGGACGTAGATCTGCAAACCCAGTTCATCCCATCACATTATCCAACGCATGTGCAAGCGCCCAGATCGGCAATTCCCAGCTCTACAAACTTACAGATCTACAATCCAGATGGAGCTTTATTGCAAGTGACAGAAAGGAATTATGCATGTGCACTATGCAACAGGAGCATGGGGAAGTTCTAGCAGATTGCATGGGAACCCAAAAGGCACATTCATCTCCTCCAACCCCTGAATGCGTACATGAGGTCTTCCTGAGTTTTAGGGGCGAGGACACCCGCGATGGGTTTACGAAATATTTGTACACTGCTTTGAAAAGGAAAGGAATCAATACATTTCGGGATGACGATAAACTTGAGAGGGGAAAGACCATTAAACCAGAACTCTTGAAAGCAATTGAGGAATCGAGGTTGGCAGTCGTCATTCTTTCAGATGACTTTGCTTCTTCAACCTGGTGTTTGGATGAGCTTGCGCATATTGTAAAGTGCAATGAAGACAAGGGGCTTCAAATTTTTCCAGTTTTCTATTACGTTGATCCATCTGAGGTAAGAAAGCAAACAACAGGAGATTTTGGCAAGGCCCTTGCTAAGCATCATGAAGATTTCAAGAACGAAATCGGGAAGGTGGAAAGGTGGAGGGAAGCTTTAAAGTATGTATCGAATGTCGCTGGATGGCATATTCAGAAAGAAAGGTCTTATGAGTCAGAAATTATTCAGGACATTGCTCAAAAGATTTTAGATCTGTTGAAACCGTTACGTGCAAACAGGGATTTGATTGGAATAGATTCTCGGCTAAAGAAAGTGATGCAGTTGTGCTTTGATCCAGCGCCATTATCCAATGATGATGTTCGCACAATAGGGATATGGGGAGTTGGGGGCATAGGAAAGACAATTCTGGCACGACAAGCTTTTGAAAAGATCGTGTTAGAGAGGAATGAGGAATTTAAACACATATGCTTTCTTGACCGTGTAAGAGAGGAATGCGGAAAACATGGCCGAGGTCTAGTTTACTTGCAAAATTATCTTTTTAAGACTCTGCCAGACAGCAATAAAGATGATCGCATACAAGACATCCCTAAAGGGAAAGAGCTGTTAAGTGCAAGACTCCGTTTCCATAAGGTGCTCATCGTACTGGACGATGTTGATGATTTTGAACAGATCAAAGGTTTAGCAGATTGTTCTTGGTTGAGTCCAGGAAGTCGAGTCATTGTAACAACCAGAGACAAGACGACGTTAAAGAAGTGTCAAGTAAATGATGATAAAATATACGAGGTTGAGAAACTGACTCCTGGAGAAGCTTTTCAGCTCTTCTGTCGGAAAGCCTTTAAGAAAAGTCATGCTCCAGATGATCATTATAAAGACCTGTCCGACAAATTTGTCGAGTATGCCAGTGGCCTCCCATTGGCTCTTCAAGTGCTAGGTTCATACTTGAATGAGGAAAAAGATTGGGCAGAAGTGTTGGATAAACTTAAAGAGTATCCTGAAAAAGGCATTGATGGTGTGCTTGAAATAAGTTGTCGTGGATTAGAGCACACACAAAGGGAAATCTTTTTAGACATTGCGTGCTTCTTCAATGGAGAGGATGAAGTTCGTGTTAAAAAGATACTAGAAAGTTGTGGCTTCTATCCAAACAATGGTATACTTGACCTCGTTGATAAGTCTCTAATTACAATTGAGCACAATAAACTGTGGATGCATGATTTACTACAACAAACGGGTCAACAAATTGCTGTCAAAGAATTTCCTAAAGAGCCGGGAAAACGTAGCAGGTTGTGGGTTAATAACAAGGCTTACAAAGACAAAGAGTCGTGGCATAATGAAGTACTCACAGAAAATACTGGAACAAATCTTGTTGAAGGCATGTTCCTATGCTTGCCTGAGATGAGACAAATAAAGTTGGATGCTGACCCATTCTCAAAAATGTACAACCTAAGATTGTTGAAGATTTGTAATGCGAACTTTTCTAAATGCCCTGAATATTTCTCTAACAAGTTACGGCTTTTGGAATGGCATGAATATCCGTTAGAATGTCTGCCATCGAGTTTTGGACCACATCAGCTCGTTGAACTCAAGATGCCTAACAGCCGCATTAAGCAGCTATGGAATGAAAGGCTTCCGCTGATGGAAAATTTGGTGCAAATCGATCGATCCAACTCTCAAAGCTTGACAAAGACTCTGGACTTCAGTATGGTCCCAAATCTAGAGGAGCTGATCCTTGAAGGCTGTGTGAAATTAGTAGAGCTTCACCCGACAATTGGGAACCTCCAACACCTTCGTTCATTGAATTTAAAGGGTTGTGAAAGTCTAGAGAGCCTTCCCCCTATCAGCTTGGGATCTCTCAAAACTCTTATTCTTTCGGGGTGTTCAAAACTCACTAAGTTCCCAGAGATTGTGGGAAGTATGGAAAAGTTGTCACGACTTTATTTAGATGGAACAGCTATAAGAGAGCTGCCGGTAACATTCGAGAAGTTGAGAGGTCTTATTTTGTTAAATCTGAAAGGCTGCAAGAACCTTCATTTGAGTCGTTCAAGCATCCTTGGTAGTTTGACATCTCTGAAAGATCTCAATTTGTCTGGCTGCTCGCATGTTGAACTGCCTGAAAACATAGGGAACTTGCAACATTTGGAGGAGCTTGATGCCTGTGACAGCGGCATAAGAAAAGTTCCCTCCTCCATTTCACGTCTGAATAAGCTTAAACTATTATGTTTAAATAGATGTAAAGATGAGCAAATGGGTTTGCAGTTGCCTAACTCTTTCTCAGGTTTGACCTCATTGTATAGATTAAATCTAGGTCAATGCGGTCTTGAGGAAAGAGCCATCCCTGATGATATTGACTGCTTATCTTCATTGTTGTATTTGGATTTGAGTGAAAACAATTTTGTGAGCTTACCAGCAGGCATCTCTCAACTTCCTGAGCTTAAACAACTTTCCTTGTTTAGGTGCAGAAATCTAGAGTTGTTACCAAAAACTCTTCCATCCAAGCTAACACATGTATCTGCTCGTGAGTGCCCAATGTTGAAAAATTATTCAAGTAATTTGACAATACGGGCTGATGGCGGTTTCTGTCACATAGATTGTGGAAACCCTTGCGAAAATGAAGAGAACGAAGACTATGACCATTTTCCACTTCCAATTCCAGAAGACCATGCTGAAACACACTTTCCTAAGTACATTGAGGAGCGAATGAAAAGTGAAGAGTCGTTTGAATTGCGTTTTCCTCACAGTGCTATAATTCCCGACTGGTGTAGTGAACATTCAAGTCGGGATTCTGTAACCATCCAACTATCTGATAGCACGTGGATAGGGTTTGCCCTGTTTGTTGTTTTTGAACTTCTCGTGAAGGAAGATTTTGATAACAGTTGGGAAATGAAAGACACTTTTTGCCATTTTAGCACCTCAGAAGATCCTCTAGTCCTTCATAAGTTTAATAATTTCAGAAAGGGGTCGTATGGACTTTGTTGCTATCAGCCACGAGGCAGGCAATTTGCACGGCAATTGAGTACAGAAAGTAGGCTACTTAAAGCTTCAGTTTCAACAAATAGACCTGATCTGAAAGTAAAAGGGTGCGCATTACAACTAATATACCAAAAAGATGCTGCAGCGTTTGTTCGCGATTTATCAGAATCTGCTAATGGACATCTGAATTTGAATTTTGGTCGACATTGTAAGGGAATATTGGATCGGGCAAGGGAACTGAGCAACTCTGGTGAGGTGACCGTAATTGGCTCTAATCTACAAGAGCTAGGATCAAATTCAACTTCAAATCAAGGCAGCAGTAGCCAAAGTACTACTTACTCTAACATCGAGCAGTCAAATGGGGAGTTACAGCTGTTGCTTTCAGCACTGTATGAGGGATTCAATGGACGCGAGAAGAATTTTCATTTTATTTTTTCTCATCCTATAATTTCACGCTGGTTCATCCATCACTATGCTGGAGGCGTAGCACTTTGTCACCTTCCTCAACACTTATATGATGACAGGAGATGGGTAGGATTCGAACTATATGTTGTCCTTACCTGGTCTTCATCTGATTACAACAATCCTCCCCATTCTGCACGGCTCCATGTTGATTTGTGTTCCCCTGAAGGTCGTATAATGCATAGGTGCATCAAAATACACAACCTTGGACAACAGCAACTAGTTGTTCTGCACATCCCACGTGTTTGTTTTTCACAACACGAGTTGGATCAATGTCAAGGTGTTTGCGCTTTGTTTGGAACCAGTACCCTAGACGTGGAAGTCAAAGTGTGTGGGAGCCGTCTGGCATATGAGCAAGACTTGAAAGAGTTAATCAAAGCATTAGCAACTGAATGCACATTCAAGGGACCACTCGACCTACTTTATGTGCAGGCACGAACATATGTTGAAAACATGGTGGAAAGTCCACTTCGTGAAGCTTTAACGGTTCAACGTGAAGCACTAGTACCTCAATTCAGAGAAATCTGGGAAGTTTCTAAATTGTTCTGGCAACTTGTGATCGATCTGCTTCTGAACTCAGGTCCTAAAGAACAAGTACATGAACAAGATTTCACTTCAAGATTGGACTACTACATGTGCTATCTCGAACAAAAGCAAGTCCTTATGGAACCACTTGGCCATCTACTTGTCCATTCCAAACACTATCTTTTGGAAAATAGGGATTCCATCCAACAACATGATGCGGAAAGATATTCAGAATCATTGGATAGACAGTTATACATCATGGCTGAGAATCAACTCTCTGGAAGCTATAATATAGCTGAGTGGAAAAAAAGTATTGTGGTGTTGCTTCAAATTTGGAAGGAGGCCATTCTCAGTCTCAAAGGACATACAATCTCTGTTTTGAAGCATTTCGATCCTTCCGCACCATACAACTTTTGTTTCCCTCAGAAGCAAATTCTAGAGTGGTTTAAAAATCAGAGTAGTAAGTCATCCAAGATCGGAATGGAACTACCTCAGAATCTGTACGATGATGATAATTGGAGGGGACTTGTGATATGTGCTGCATTTTCAGTCCATGAGCTTCCAACTGCTACCCTTGAAAAACTGGGTTCAGAAATGACTGTCGAACTTATGTGTCACTTAAGTACTGAGGAGTATTGCTTGAACCCTATTCCCATTTGTTCCACAACTAAAGAGAAATTCAAGTGGTTGCATCTCCGCGGATTTATCTGGCTGACTTACATACCCCGAAATTTCCTGGCAGAATTCAAGGACATAACACTTGTACAAGCCAGAATTTATCATGGTTGCCCAGGCTTGACTGTCGAGGATACCGGTATTCGTCTCTTATACAAGCACGAAGTGGAGGAGCTAAAGCAAGCAATGACCCAGTGCTGGACCTCCTTCTTTGACAATTCAGACCTCATCCGTCAATTCGTAGAAGAAGATGAAATTATTCAGTCGAGCGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

1916

Amino Acids

219.14

Weight (kDa)

6.32

Isoelectric Point (pI)

43.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000307)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g16600 FvH4_1g16610 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16621 FvH4_1g16630 FvH4_1g16640 FvH4_1g16640 FvH4_1g16650 FvH4_1g16650 FvH4_3g27631 FvH4_4g11392 FvH4_4g11542
prunus_persica Prupe.4G224500_v2.0.a1 Prupe.4G226900_v2.0.a1 Prupe.4G227000_v2.0.a1
pyrus_communis pycom11g18180 pycom11g18210 pycom11g18230 pycom11g18270 pycom11g18350 pycom11g18360 pycom11g18370
rosa_chinensis RchiOBHm_Chr2g0106511 RchiOBHm_Chr2g0106541 RchiOBHm_Chr2g0106551 RchiOBHm_Chr2g0106571 RchiOBHm_Chr2g0124621 RchiOBHm_Chr6g0251591 RchiOBHm_Chr6g0251631 RchiOBHm_Chr6g0251641
rosa_laevigata RLG00000015443 RLG00000015444 RLG00000015458 RLG00000017510 RLG00000017511 RLG00000017512 RLG00000017515 RLG00000017516 RLG00000017517 RLG00000018809 RLG00000020934 RLG00000020935 RLG00000034092
rosa_multiflora Rmu_co8285777.1_g000001 Rmu_sc0000762.1_g000001 Rmu_sc0001872.1_g000006 Rmu_sc0005255.1_g000019 Rmu_sc0005597.1_g000006 Rmu_sc0012104.1_g000001 Rmu_sc0017105.1_g000011 Rmu_sc0022904.1_g000001 Rmu_ssc0000135.1_g000049 Rmu_ssc0000213.1_g000047
rosa_roxburghii Rroxscaffold_2G00092830 Rroxscaffold_2G00119790 Rroxscaffold_2G00137010 Rroxscaffold_2G00137030 Rroxscaffold_2G00137070 Rroxscaffold_7G00216560
rosa_rugosa Rorug02G0136100 Rorug02G0136200 Rorug02G0136300 Rorug02G0136400 Rorug02G0251800 Rorug02G0251800 Rorug02G0455500 Rorug02G0455600 Rorug02G0455700 Rorug05G0195300
rosa_samantha Rh2AG187400 Rh2AG187500 Rh2AG187600 Rh2AG310500 Rh2BG198200 Rh2BG198300 Rh2BG198400 Rh2BG198600 Rh2BG318900 Rh2BG534300 Rh2CG192400 Rh2CG192500 Rh2CG192600 Rh2CG297500 Rh2DG193500 Rh2DG193600 Rh2DG193800 Rh2DG334200 Rh2DG543100 Rh5AG280400 Rh5BG285800 Rh5BG285900 Rh5CG317900 Rh5CG318000 Rh5DG294600 Rh5DG294700 Rh6AG012200 Rh6AG012700 Rh6BG011200 Rh6BG011700
rosa_wichuraiana Rw0G009380 Rw2G014610 Rw2G014630 Rw2G014640 Rw2G014680 Rw2G025120 Rw2G043000 Rw5G026490 Rw6G001370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 138, 1710
AasI GACNNNNNNGTC 2 cut(s) 1730, 4592
Acc16I TGCGCA 2 cut(s) 828, 3922
Acc36I ACCTGC 1 cut(s) 2112
AccB7I CCANNNNNTGG 1 cut(s) 1988
AccBSI CCGCTC 1 cut(s) 5742
AccI GTMKAC 2 cut(s) 1993, 4012
AccII CGCG 4 cut(s) 636, 3966, 4221, 5496
AcoI YGGCCR 2 cut(s) 1348, 4948
AcuI CTGAAG 3 cut(s) 2521, 3864, 4458
AcvI CACGTG 2 cut(s) 3651, 4514
AdeI CACNNNGTG 2 cut(s) 4745, 5638
AfiI CCNNNNNNNGG 8 cut(s) 639, 1756, 1841, 1988, 2609, 3291, 4588, 5670
AflII CTTAAG 1 cut(s) 5416
AflIII ACRYGT 3 cut(s) 3343, 4513, 4905
AgeI ACCGGT 1 cut(s) 5609
AhdI GACNNNNNGTC 1 cut(s) 2158
AhlI ACTAGT 2 cut(s) 4492, 4774
AjiI CACGTC 2 cut(s) 3035, 4588
AjnI CCWGG 7 cut(s) 355, 806, 1546, 1642, 4370, 5532, 5587
Alw21I GWGCWC 4 cut(s) 512, 1479, 1887, 2034
Alw26I GTCTC 6 cut(s) 268, 1569, 2019, 2233, 5156, 5624
Alw44I GTGCAC 1 cut(s) 508
AlwNI CAGNNNCTG 2 cut(s) 3980, 5296
AoxI GGCC 5 cut(s) 933, 1348, 1747, 4948, 5139
ApaLI GTGCAC 1 cut(s) 508
ApeKI GCWGC 9 cut(s) 338, 2449, 2803, 2856, 2931, 3950, 3953, 4125, 5336
AsiGI ACCGGT 1 cut(s) 5609
Asp700I GAANNNNTTC 6 cut(s) 1065, 1138, 1653, 2466, 5183, 5730
AspLEI GCGC 6 cut(s) 273, 419, 829, 1183, 3923, 4562
AspS9I GGNCC 6 cut(s) 934, 2402, 2545, 4679, 4853, 5673
AsuC2I CCSGG 1 cut(s) 2109
AsuHPI GGTGA 5 cut(s) 61, 2591, 4073, 4078, 4298
AsuII TTCGAA 1 cut(s) 4350
AvaII GGWCC 5 cut(s) 2402, 2545, 4679, 4853, 5673
BaeGI GKGCMC 2 cut(s) 512, 3365
BaeI ACNNNNGTAYC 2 cut(s) 3330, 3363
BalI TGGCCA 1 cut(s) 4950
BarI GAAGNNNNNNTAC 2 cut(s) 4761, 4793
BauI CACGAG 4 cut(s) 3354, 3689, 3825, 4532
BbrPI CACGTG 2 cut(s) 3651, 4514
BbsI GAAGAC 5 cut(s) 598, 855, 3468, 3501, 4368
Bbv12I GWGCWC 4 cut(s) 512, 1479, 1887, 2034
BbvI GCAGC 9 cut(s) 325, 2461, 2790, 2843, 2918, 3937, 3965, 4137, 5323
BceAI ACGGC 4 cut(s) 2366, 3860, 4427, 4596
BcgI CGANNNNNNTGC 2 cut(s) 2480, 2514
BciT130I CCWGG 7 cut(s) 357, 808, 1548, 1644, 4372, 5534, 5589
BciVI GTATCC 2 cut(s) 1842, 5599
BclI TGATCA 1 cut(s) 1702
BcnI CCSGG 1 cut(s) 2109
BcoDI GTCTC 6 cut(s) 268, 1569, 2019, 2233, 5156, 5624
BcuI ACTAGT 2 cut(s) 4492, 4774
BfmI CTRYAG 1 cut(s) 3951
BfoI RGCGCY 2 cut(s) 420, 1184
BfrI CTTAAG 1 cut(s) 5416
BfuAI ACCTGC 1 cut(s) 2112
BfuI GTATCC 2 cut(s) 1842, 5599
BglII AGATCT 4 cut(s) 368, 454, 1093, 2914
BlpI GCTNAGC 1 cut(s) 942
BmcAI AGTACT 3 cut(s) 2178, 4141, 5422
Bme1390I CCNGG 8 cut(s) 357, 808, 1548, 1644, 2109, 4372, 5534, 5589
Bme18I GGWCC 5 cut(s) 2402, 2545, 4679, 4853, 5673
BmeRI GACNNNNNGTC 1 cut(s) 2158
BmgBI CACGTC 2 cut(s) 3035, 4588
BmgT120I GGNCC 6 cut(s) 934, 2402, 2545, 4679, 4853, 5673
BmiI GGNNCC 8 cut(s) 556, 2547, 2615, 4415, 4573, 4610, 4680, 4941
BmrFI CCNGG 8 cut(s) 357, 808, 1548, 1644, 2109, 4372, 5534, 5589
BmrI ACTGGG 3 cut(s) 374, 5390, 5659
BmuI ACTGGG 3 cut(s) 374, 5390, 5659
BpiI GAAGAC 5 cut(s) 598, 855, 3468, 3501, 4368
BpmI CTGGAG 4 cut(s) 378, 1665, 1680, 4308
Bpu10I CCTNAGC 1 cut(s) 3267
Bpu1102I GCTNAGC 1 cut(s) 942
Bpu14I TTCGAA 1 cut(s) 4350
BpuEI CTTGAG 3 cut(s) 731, 2408, 3179
BpuMI CCSGG 1 cut(s) 2109
Bsa29I ATCGAT 3 cut(s) 2496, 2500, 4833
BsaAI YACGTR 3 cut(s) 1112, 3651, 4514
BsaJI CCNNGG 5 cut(s) 1351, 2890, 4477, 5494, 5587
BsaWI WCCGGW 1 cut(s) 5609
Bsc4I CCNNNNNNNGG 8 cut(s) 639, 1756, 1841, 1988, 2609, 3291, 4588, 5670
Bse118I RCCGGY 2 cut(s) 2806, 5609
Bse1I ACTGG 9 cut(s) 380, 872, 1491, 1743, 2197, 3599, 4575, 5385, 5665
Bse3DI GCAATG 4 cut(s) 850, 1074, 1911, 5664
BseBI CCWGG 7 cut(s) 357, 808, 1548, 1644, 4372, 5534, 5589
BseCI ATCGAT 3 cut(s) 2496, 2500, 4833
BseDI CCNNGG 5 cut(s) 1351, 2890, 4477, 5494, 5587
BseLI CCNNNNNNNGG 8 cut(s) 639, 1756, 1841, 1988, 2609, 3291, 4588, 5670
BseMI GCAATG 4 cut(s) 850, 1074, 1911, 5664
BseNI ACTGG 9 cut(s) 380, 872, 1491, 1743, 2197, 3599, 4575, 5385, 5665
BseRI GAGGAG 8 cut(s) 301, 567, 2573, 2996, 3013, 3545, 5441, 5657
BseSI GKGCMC 2 cut(s) 512, 3365
BseXI GCAGC 9 cut(s) 325, 2461, 2790, 2843, 2918, 3937, 3965, 4137, 5323
BseYI CCCAGC 1 cut(s) 435
BsgI GTGCAG 4 cut(s) 3316, 4392, 4487, 4721
Bsh1236I CGCG 4 cut(s) 636, 3966, 4221, 5496
Bsh1285I CGRYCG 1 cut(s) 2500
BshFI GGCC 5 cut(s) 935, 1350, 1749, 4950, 5141
BshTI ACCGGT 1 cut(s) 5609
BshVI ATCGAT 3 cut(s) 2496, 2500, 4833
BsiEI CGRYCG 1 cut(s) 2500
BsiHKAI GWGCWC 4 cut(s) 512, 1479, 1887, 2034
BsiSI CCGG 3 cut(s) 2108, 2807, 5610
BslFI GGGAC 3 cut(s) 2531, 4692, 5334
BslI CCNNNNNNNGG 8 cut(s) 639, 1756, 1841, 1988, 2609, 3291, 4588, 5670
BsmAI GTCTC 6 cut(s) 268, 1569, 2019, 2233, 5156, 5624
BsmBI CGTCTC 2 cut(s) 268, 5624
BsmFI GGGAC 3 cut(s) 2531, 4692, 5334
BsmI GAATGC 3 cut(s) 596, 1340, 4670
BsnI GGCC 5 cut(s) 935, 1350, 1749, 4950, 5141
Bsp119I TTCGAA 1 cut(s) 4350
Bsp1286I GDGCHC 5 cut(s) 512, 1479, 1887, 2034, 3365
Bsp1407I TGTACA 3 cut(s) 657, 2277, 5560
Bsp1720I GCTNAGC 1 cut(s) 942
Bsp68I TCGCGA 1 cut(s) 3966
BspANI GGCC 5 cut(s) 935, 1350, 1749, 4950, 5141
BspDI ATCGAT 3 cut(s) 2496, 2500, 4833
BspFNI CGCG 4 cut(s) 636, 3966, 4221, 5496
BspHI TCATGA 1 cut(s) 949
BspLI GGNNCC 8 cut(s) 556, 2547, 2615, 4415, 4573, 4610, 4680, 4941
BspMAI CTGCAG 1 cut(s) 3955
BspMI ACCTGC 1 cut(s) 2112
BspQI GCTCTTC 2 cut(s) 1667, 1767
BspT104I TTCGAA 1 cut(s) 4350
BspTI CTTAAG 1 cut(s) 5416
BsrBI CCGCTC 1 cut(s) 5742
BsrDI GCAATG 4 cut(s) 850, 1074, 1911, 5664
BsrFI RCCGGY 2 cut(s) 2806, 5609
BsrGI TGTACA 3 cut(s) 657, 2277, 5560
BsrI ACTGG 9 cut(s) 380, 872, 1491, 1743, 2197, 3599, 4575, 5385, 5665
BssAI RCCGGY 2 cut(s) 2806, 5609
BssECI CCNNGG 5 cut(s) 1351, 2890, 4477, 5494, 5587
BssNAI GTATAC 1 cut(s) 1994
BssSI CACGAG 4 cut(s) 3354, 3689, 3825, 4532
BssT1I CCWWGG 2 cut(s) 2890, 4477
Bst1107I GTATAC 1 cut(s) 1994
Bst2BI CACGAG 4 cut(s) 3354, 3689, 3825, 4532
Bst2UI CCWGG 7 cut(s) 357, 808, 1548, 1644, 4372, 5534, 5589
Bst6I CTCTTC 5 cut(s) 1667, 1767, 3330, 3447, 3543
BstAFI CTTAAG 1 cut(s) 5416
BstAUI TGTACA 3 cut(s) 657, 2277, 5560
BstBAI YACGTR 3 cut(s) 1112, 3651, 4514
BstBI TTCGAA 1 cut(s) 4350
BstC8I GCNNGC 9 cut(s) 415, 825, 1918, 2228, 2936, 3250, 3833, 4704, 5655
BstDSI CCRYGG 1 cut(s) 5494
BstEII GGTNACC 1 cut(s) 4066
BstENI CCTNNNNNAGG 2 cut(s) 1839, 3289
BstFNI CGCG 4 cut(s) 636, 3966, 4221, 5496
BstH2I RGCGCY 2 cut(s) 420, 1184
BstHHI GCGC 6 cut(s) 273, 419, 829, 1183, 3923, 4562
BstMAI GTCTC 6 cut(s) 268, 1569, 2019, 2233, 5156, 5624
BstMCI CGRYCG 1 cut(s) 2500
BstMWI GCNNNNNNNGC 4 cut(s) 523, 1259, 3744, 4131
BstNI CCWGG 7 cut(s) 357, 808, 1548, 1644, 4372, 5534, 5589
BstNSI RCATGY 6 cut(s) 411, 508, 2218, 2942, 3347, 4909
BstPI GGTNACC 1 cut(s) 4066
BstSCI CCNGG 8 cut(s) 355, 806, 1546, 1642, 2107, 4370, 5532, 5587
BstSFI CTRYAG 1 cut(s) 3951
BstSLI GKGCMC 2 cut(s) 512, 3365
BstUI CGCG 4 cut(s) 636, 3966, 4221, 5496
BstV1I GCAGC 9 cut(s) 325, 2461, 2790, 2843, 2918, 3937, 3965, 4137, 5323
BstV2I GAAGAC 5 cut(s) 598, 855, 3468, 3501, 4368
BstX2I RGATCY 6 cut(s) 368, 454, 1093, 2687, 2914, 3757
BstYI RGATCY 6 cut(s) 368, 454, 1093, 2687, 2914, 3757
BstZ17I GTATAC 1 cut(s) 1994
Bsu15I ATCGAT 3 cut(s) 2496, 2500, 4833
BsuI GTATCC 2 cut(s) 1842, 5599
BsuRI GGCC 5 cut(s) 935, 1350, 1749, 4950, 5141
BsuTUI ATCGAT 3 cut(s) 2496, 2500, 4833
BtgI CCRYGG 1 cut(s) 5494
BtgZI GCGATG 1 cut(s) 651
BtrI CACGTC 2 cut(s) 3035, 4588
BtsI GCAGTG 1 cut(s) 660
BtsIMutI CAGTG 5 cut(s) 660, 1750, 3583, 4196, 5672
BtuMI TCGCGA 1 cut(s) 3966
BveI ACCTGC 1 cut(s) 2112
Cac8I GCNNGC 9 cut(s) 415, 825, 1918, 2228, 2936, 3250, 3833, 4704, 5655
CaiI CAGNNNCTG 2 cut(s) 3980, 5296
CciI TCATGA 1 cut(s) 949
CfoI GCGC 6 cut(s) 273, 419, 829, 1183, 3923, 4562
Cfr10I RCCGGY 2 cut(s) 2806, 5609
Cfr13I GGNCC 6 cut(s) 934, 2402, 2545, 4679, 4853, 5673
Cfr42I CCGCGG 1 cut(s) 5497
ClaI ATCGAT 3 cut(s) 2496, 2500, 4833
CseI GACGC 2 cut(s) 344, 4227
CsiI ACCWGGT 2 cut(s) 806, 4370
CspAI ACCGGT 1 cut(s) 5609
CspCI CAANNNNNGTGG 2 cut(s) 4585, 4620
DraI TTTAAA 7 cut(s) 15, 28, 1002, 1300, 2643, 3063, 5245
DraIII CACNNNGTG 2 cut(s) 4745, 5638
DrdI GACNNNNNNGTC 2 cut(s) 1730, 4592
DriI GACNNNNNGTC 1 cut(s) 2158
DseDI GACNNNNNNGTC 2 cut(s) 1730, 4592
EaeI YGGCCR 2 cut(s) 1348, 4948
Eam1104I CTCTTC 5 cut(s) 1667, 1767, 3330, 3447, 3543
Eam1105I GACNNNNNGTC 1 cut(s) 2158
EarI CTCTTC 5 cut(s) 1667, 1767, 3330, 3447, 3543
Eco130I CCWWGG 2 cut(s) 2890, 4477
Eco47I GGWCC 5 cut(s) 2402, 2545, 4679, 4853, 5673
Eco57I CTGAAG 3 cut(s) 2521, 3864, 4458
Eco72I CACGTG 2 cut(s) 3651, 4514
Eco91I GGTNACC 1 cut(s) 4066
EcoNI CCTNNNNNAGG 2 cut(s) 1839, 3289
EcoO109I RGGNCCY 2 cut(s) 934, 4853
EcoO65I GGTNACC 1 cut(s) 4066
EcoRI GAATTC 2 cut(s) 60, 5540
EcoRII CCWGG 7 cut(s) 355, 806, 1546, 1642, 4370, 5532, 5587
EcoT14I CCWWGG 2 cut(s) 2890, 4477
EcoT22I ATGCAT 3 cut(s) 506, 2052, 4455
ErhI CCWWGG 2 cut(s) 2890, 4477
Esp3I CGTCTC 2 cut(s) 268, 5624
FalI AAGNNNNNCTT 2 cut(s) 4129, 4161
FaqI GGGAC 3 cut(s) 2531, 4692, 5334
FauI CCCGC 1 cut(s) 641
FauNDI CATATG 3 cut(s) 1307, 4621, 4714
FbaI TGATCA 1 cut(s) 1702
FblI GTMKAC 2 cut(s) 1993, 4012
FspAI RTGCGCAY 1 cut(s) 3922
FspI TGCGCA 2 cut(s) 828, 3922
GlaI GCGC 6 cut(s) 272, 418, 828, 1182, 3922, 4561
GsaI CCCAGC 1 cut(s) 439
GsuI CTGGAG 4 cut(s) 378, 1665, 1680, 4308
HaeII RGCGCY 2 cut(s) 420, 1184
HaeIII GGCC 5 cut(s) 935, 1350, 1749, 4950, 5141
HapII CCGG 3 cut(s) 2108, 2807, 5610
HgaI GACGC 2 cut(s) 344, 4227
HhaI GCGC 6 cut(s) 273, 419, 829, 1183, 3923, 4562
Hin6I GCGC 6 cut(s) 271, 417, 827, 1181, 3921, 4560
HinP1I GCGC 6 cut(s) 271, 417, 827, 1181, 3921, 4560
HincII GTYRAC 2 cut(s) 2075, 4013
HindII GTYRAC 2 cut(s) 2075, 4013
HindIII AAGCTT 8 cut(s) 151, 996, 1260, 1650, 2515, 3044, 3876, 4749
HpaII CCGG 3 cut(s) 2108, 2807, 5610
HphI GGTGA 5 cut(s) 61, 2591, 4073, 4078, 4298
Hpy166II GTNNAC 9 cut(s) 510, 645, 660, 1363, 1994, 2075, 3605, 4013, 4739
Hpy8I GTNNAC 9 cut(s) 510, 645, 660, 1363, 1994, 2075, 3605, 4013, 4739
Hpy99I CGWCG 3 cut(s) 263, 338, 1588
HpyF10VI GCNNNNNNNGC 4 cut(s) 523, 1259, 3744, 4131
HspAI GCGC 6 cut(s) 271, 417, 827, 1181, 3921, 4560
Ksp22I TGATCA 1 cut(s) 1702
KspI CCGCGG 1 cut(s) 5497
LguI GCTCTTC 2 cut(s) 1667, 1767
Lsp1109I GCAGC 9 cut(s) 325, 2461, 2790, 2843, 2918, 3937, 3965, 4137, 5323
MabI ACCWGGT 2 cut(s) 806, 4370
MbiI CCGCTC 1 cut(s) 5742
MfeI CAATTG 4 cut(s) 750, 2025, 2607, 3848
MflI RGATCY 6 cut(s) 368, 454, 1093, 2687, 2914, 3757
MhlI GDGCHC 5 cut(s) 512, 1479, 1887, 2034, 3365
MlsI TGGCCA 1 cut(s) 4950
MluNI TGGCCA 1 cut(s) 4950
Mox20I TGGCCA 1 cut(s) 4950
Mph1103I ATGCAT 3 cut(s) 506, 2052, 4455
MroXI GAANNNNTTC 6 cut(s) 1065, 1138, 1653, 2466, 5183, 5730
MscI TGGCCA 1 cut(s) 4950
MslI CAYNNNNRTG 2 cut(s) 4281, 4323
Msp20I TGGCCA 1 cut(s) 4950
MspA1I CMGCKG 4 cut(s) 2473, 3003, 4183, 5496
MspCI CTTAAG 1 cut(s) 5416
MspI CCGG 3 cut(s) 2108, 2807, 5610
MspR9I CCNGG 8 cut(s) 357, 808, 1548, 1644, 2109, 4372, 5534, 5589
MunI CAATTG 4 cut(s) 750, 2025, 2607, 3848
Mva1269I GAATGC 3 cut(s) 596, 1340, 4670
MvaI CCWGG 7 cut(s) 357, 808, 1548, 1644, 4372, 5534, 5589
MvnI CGCG 4 cut(s) 636, 3966, 4221, 5496
MwoI GCNNNNNNNGC 4 cut(s) 523, 1259, 3744, 4131
NciI CCSGG 1 cut(s) 2109
NdeI CATATG 3 cut(s) 1307, 4621, 4714
NlaIV GGNNCC 8 cut(s) 556, 2547, 2615, 4415, 4573, 4610, 4680, 4941
NmeAIII GCCGAG 2 cut(s) 1123, 1376
NmuCI GTSAC 7 cut(s) 485, 2766, 2996, 3419, 4066, 4304, 5411
NruI TCGCGA 1 cut(s) 3966
NsbI TGCGCA 2 cut(s) 828, 3922
NsiI ATGCAT 3 cut(s) 506, 2052, 4455
NspI RCATGY 6 cut(s) 411, 508, 2218, 2942, 3347, 4909
NspV TTCGAA 1 cut(s) 4350
PagI TCATGA 1 cut(s) 949
PciI ACATGT 2 cut(s) 3343, 4905
PciSI GCTCTTC 2 cut(s) 1667, 1767
PcsI WCGNNNNNNNCGW 1 cut(s) 1488
PctI GAATGC 3 cut(s) 596, 1340, 4670
PdmI GAANNNNTTC 6 cut(s) 1065, 1138, 1653, 2466, 5183, 5730
PflMI CCANNNNNTGG 1 cut(s) 1988
PfoI TCCNGGA 2 cut(s) 1546, 1642
PinAI ACCGGT 1 cut(s) 5609
Ple19I CGATCG 1 cut(s) 2500
PmaCI CACGTG 2 cut(s) 3651, 4514
PmlI CACGTG 2 cut(s) 3651, 4514
Ppu21I YACGTR 3 cut(s) 1112, 3651, 4514
PpuMI RGGWCCY 1 cut(s) 4853
PscI ACATGT 2 cut(s) 3343, 4905
PsiI TTATAA 2 cut(s) 138, 1710
Psp5II RGGWCCY 1 cut(s) 4853
Psp6I CCWGG 7 cut(s) 355, 806, 1546, 1642, 4370, 5532, 5587
PspCI CACGTG 2 cut(s) 3651, 4514
PspEI GGTNACC 1 cut(s) 4066
PspFI CCCAGC 1 cut(s) 435
PspGI CCWGG 7 cut(s) 355, 806, 1546, 1642, 4370, 5532, 5587
PspN4I GGNNCC 8 cut(s) 556, 2547, 2615, 4415, 4573, 4610, 4680, 4941
PspPI GGNCC 6 cut(s) 934, 2402, 2545, 4679, 4853, 5673
PspPPI RGGWCCY 1 cut(s) 4853
PstI CTGCAG 1 cut(s) 3955
PstNI CAGNNNCTG 2 cut(s) 3980, 5296
PsuI RGATCY 6 cut(s) 368, 454, 1093, 2687, 2914, 3757
PvuI CGATCG 1 cut(s) 2500
PvuII CAGCTG 1 cut(s) 4183
RruI TCGCGA 1 cut(s) 3966
RseI CAYNNNNRTG 2 cut(s) 4281, 4323
SacII CCGCGG 1 cut(s) 5497
SalI GTCGAC 1 cut(s) 4011
SapI GCTCTTC 2 cut(s) 1667, 1767
Sau96I GGNCC 6 cut(s) 934, 2402, 2545, 4679, 4853, 5673
ScaI AGTACT 3 cut(s) 2178, 4141, 5422
ScrFI CCNGG 8 cut(s) 357, 808, 1548, 1644, 2109, 4372, 5534, 5589
SduI GDGCHC 5 cut(s) 512, 1479, 1887, 2034, 3365
SexAI ACCWGGT 2 cut(s) 806, 4370
SfcI CTRYAG 1 cut(s) 3951
Sfr303I CCGCGG 1 cut(s) 5497
SfuI TTCGAA 1 cut(s) 4350
SgrBI CCGCGG 1 cut(s) 5497
SinI GGWCC 5 cut(s) 2402, 2545, 4679, 4853, 5673
SmiMI CAYNNNNRTG 2 cut(s) 4281, 4323
SmlI CTYRAG 4 cut(s) 710, 2423, 3158, 5416
SmoI CTYRAG 4 cut(s) 710, 2423, 3158, 5416
SpeI ACTAGT 2 cut(s) 4492, 4774
SspI AATATT 5 cut(s) 24, 208, 653, 2333, 4029
StyD4I CCNGG 8 cut(s) 355, 806, 1546, 1642, 2107, 4370, 5532, 5587
StyI CCWWGG 2 cut(s) 2890, 4477
TatI WGTACW 9 cut(s) 657, 2176, 2277, 3522, 3854, 4139, 4867, 5420, 5560
TauI GCSGC 2 cut(s) 2442, 3006
TscAI CASTG 5 cut(s) 667, 1750, 3583, 4203, 5672
TseFI GTSAC 7 cut(s) 485, 2766, 2996, 3419, 4066, 4304, 5411
TseI GCWGC 9 cut(s) 338, 2449, 2803, 2856, 2931, 3950, 3953, 4125, 5336
Tsp45I GTSAC 7 cut(s) 485, 2766, 2996, 3419, 4066, 4304, 5411
TspGWI ACGGA 4 cut(s) 247, 1451, 2364, 5693
TspRI CASTG 5 cut(s) 667, 1750, 3583, 4203, 5672
Van91I CCANNNNNTGG 1 cut(s) 1988
Vha464I CTTAAG 1 cut(s) 5416
VneI GTGCAC 1 cut(s) 508
VpaK11BI GGWCC 5 cut(s) 2402, 2545, 4679, 4853, 5673
XagI CCTNNNNNAGG 2 cut(s) 1839, 3289
XbaI TCTAGA 4 cut(s) 2554, 2659, 3304, 5233
XceI RCATGY 6 cut(s) 411, 508, 2218, 2942, 3347, 4909
XcmI CCANNNNNNNNNTGG 2 cut(s) 4283, 5275
XmiI GTMKAC 2 cut(s) 1993, 4012
XmnI GAANNNNTTC 6 cut(s) 1065, 1138, 1653, 2466, 5183, 5730
ZrmI AGTACT 3 cut(s) 2178, 4141, 5422
Zsp2I ATGCAT 3 cut(s) 506, 2052, 4455
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.