RLG00000015443

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000307
Physical Location & Seq
Forward (+)
66040268 .. 66047176
6909 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015443

Sequence Viewer

Length: 2367 bp
ATGTTTGCCGTCCTCCTTTTCCTCATTCTCCTTGTTTCTCTTCTTCTTCAATTTCTTAATTATCTTCTAGTACCACCTGCCCAATCTCCAGCGGATTCCCCGAACATCCCAGCAGCAGCTTCCTCTTCTTCGGGCTCAACATTGGAACATGATGTCGTATCCCATGATGTCTTCCTCAGTTTTAGAGGTGAAGACACCCGTGACAGTTTTACTGACCATTTATATCATGCTATGAAGCATCAAGGGATCGACGCATTCAGGGATACGGAAAAACTCGAGAGGGGACATTCAATTTCATCAGATCTCTTGAAAGCAATAAAAGAATCCCGAATTGCAGTCATCATTCTTTCAGCTAACTATGCTACTTCAACATGGTGTTTGGAGGAGCTTGCACAGATTGTTGAATGCGAAGAGACAACGGGGTTGAAAGTTTTACCAGTCTTTTATCACGTCAAACCAACAGAAGTAAGGAAACAAAAAGGAAATTTTCACGAGGCGTTTTGCAAGCATGAAGTGACTTTCAAAGACAACCCGGAGAAGGAGAAGGTGAAGAAGTGGAGAAAAGCTTTAACCAAAGTAGCCAGCATCTCCGGATGGGATGTAGTGAAAGGAAGAGGAGAATCAGAAGTTATTCAGACAATAACTACAGAGAAAGCTTTCAAGGAAAACCACGCTCCAGATAAATATAGGGGACTATCCAATAGTTTTGTAAAATATTGCAGTGGCCTTCCTTTAGCTCTTGTTGTTCTGGGTTCTCACTTGTATAAGAGAACGGTTGATGAATGGTCAGCAGAATTGGCTAGACTTGATGAAGATGCCCACGAAGGTATTTTTCCTGTTCTTCGATTAAGTTTTGATGGATTGAAGAATACAGAGAAAGATATCTTTTTGGACATAGCATGTTTCTTCAGAGGTGAGAGTCAATACCGTGTAAAACCGATTTTAGAAGGTTGTGGCTTCTTTCCTGATATCGGAATGTCCGTCCTCATTGAAAAACATTTAATTAAAATTGAAGAAGATAGAGTGTGGATGCATGACTTACTACAGCAAATGGGTTGGCACATTGTTCGGCAAGAATCTCCTAAGCCGGGAAAACGAAGCAGGTTGTGGCTTAATGAGAATTCCCTTAAGTATCAACGCAGCAGCCTGTGGTTTGATGAGGATACTCGTGATGTAGTCACCGAGAATCTGCTCTGTCTGGAGAAGCTAATACTGATGGATCTTTCCTACTGCCAAAACTTGGTCAACACCCCGGACTTCAGTAATTTCCCAAATCTTGAACAGTTGACTTTTGCAGGTTGTGAAAATTTATCAGATGTTCACCCAACACTTTGGAGACTCAAAAATTTGAAGGTTGCAGTTTTTCAAGATTGTGAGCGTCTAGAAAGCCTTCCCAACTCCATCAGCTTGCAATCTCTTGAAATTCTTAATCTTTCGGGATGTTCAAAACTAGATGAGTTTCCAGAGATAGTGGGAAACAGGGAAACTCTGTCAGCTCTCTATTTAGATGGAACAGCTATAAGAGAGCTGCCGACAACAATCGGGTGCTTGAGTGGCCTAATTAGGTTAAATCTCTGTGCCTGCAAGGATCTTGTGGGTCTTCCAAGCTTCATTTGCAGTTTGAAGTCTCTGAGATATCTGCGTCTGTCAAAATCCTCACGCATTGACCAACTTCCAGAAGACATAGGGAGCTTGGAAAATTTGGAGAAGCTTGATGCTTGTCACACTGCTATAAGAAAAGTGCCTTCGTCCATTGTACTTCTGAAGAATCTGAAATTATTATGCTTCCATGGATGTAGGCAGGTGCCCGGGGAATGCTCTGATGAATCCTTGGGTTTGCAATTGCCTAAGTCGTTCTCCGGTTTGAGCTCATTGGAAACACTATCTTTAGGTCAATGCGATCTTGCTGAAGGAGCGATCCCTGATGACATTGGCTGCTTATCCTCACTGAAATTTTTAAGGTTGGATGAAAACAATTTTGTGAGTATACCAGAAAGCATCTCTCAACTTCCTAACCTTAGAGAAATTCACATCCCAAATTGTAGCAAGCTTCAGTCTTTGCCAAAACGTCTTCCAAAGGGAGTTAATGTACAGGCTAGTGGATGTCCCCTGCGGACAAACTATCCAAACCAGCTTAACATATGGCTTTCTGATGAGGGGTTCCGTCGCATAAATTGTGGAAATTCAAAGCGTGCAAGCTGTCCACTTCCAATTCTCGAAGAACAAATTGAAGAATACTTACCCAAGTTTTTTGAGATGAGTTTCTACAGGGTGAAAAGTTTCCTCGGTGGGGCCTGCAGCTTCGTTACTTTCATACAGAAATTCCTGATTGGTGTCGTCACAAGAGTAGTGGTTCTTCTGTCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 LRR_8 NB-ARC TIR

Protein Analysis

789

Amino Acids

88.75

Weight (kDa)

6.08

Isoelectric Point (pI)

47.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 55 - 228 3.6e-53 TIR domain
TIR_2 PF13676 57 - 154 1.5e-15 TIR domain
WHD_ROQ1 PF23282 291 - 360 1.1e-20 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 439 - 544 1.4e-08 Leucine-rich repeat region
LRR_13 PF23286 470 - 548 1.1e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 537 - 631 8.4e-16 Leucine-rich repeat region
LRR_13 PF23286 553 - 690 4.6e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000307)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g16600 FvH4_1g16610 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16621 FvH4_1g16630 FvH4_1g16640 FvH4_1g16640 FvH4_1g16650 FvH4_1g16650 FvH4_3g27631 FvH4_4g11392 FvH4_4g11542
prunus_persica Prupe.4G224500_v2.0.a1 Prupe.4G226900_v2.0.a1 Prupe.4G227000_v2.0.a1
pyrus_communis pycom11g18180 pycom11g18210 pycom11g18230 pycom11g18270 pycom11g18350 pycom11g18360 pycom11g18370
rosa_chinensis RchiOBHm_Chr2g0106511 RchiOBHm_Chr2g0106541 RchiOBHm_Chr2g0106551 RchiOBHm_Chr2g0106571 RchiOBHm_Chr2g0124621 RchiOBHm_Chr6g0251591 RchiOBHm_Chr6g0251631 RchiOBHm_Chr6g0251641
rosa_laevigata RLG00000015443 RLG00000015444 RLG00000015458 RLG00000017510 RLG00000017511 RLG00000017512 RLG00000017515 RLG00000017516 RLG00000017517 RLG00000018809 RLG00000020934 RLG00000020935 RLG00000034092
rosa_multiflora Rmu_co8285777.1_g000001 Rmu_sc0000762.1_g000001 Rmu_sc0001872.1_g000006 Rmu_sc0005255.1_g000019 Rmu_sc0005597.1_g000006 Rmu_sc0012104.1_g000001 Rmu_sc0017105.1_g000011 Rmu_sc0022904.1_g000001 Rmu_ssc0000135.1_g000049 Rmu_ssc0000213.1_g000047
rosa_roxburghii Rroxscaffold_2G00092830 Rroxscaffold_2G00119790 Rroxscaffold_2G00137010 Rroxscaffold_2G00137030 Rroxscaffold_2G00137070 Rroxscaffold_7G00216560
rosa_rugosa Rorug02G0136100 Rorug02G0136200 Rorug02G0136300 Rorug02G0136400 Rorug02G0251800 Rorug02G0251800 Rorug02G0455500 Rorug02G0455600 Rorug02G0455700 Rorug05G0195300
rosa_samantha Rh2AG187400 Rh2AG187500 Rh2AG187600 Rh2AG310500 Rh2BG198200 Rh2BG198300 Rh2BG198400 Rh2BG198600 Rh2BG318900 Rh2BG534300 Rh2CG192400 Rh2CG192500 Rh2CG192600 Rh2CG297500 Rh2DG193500 Rh2DG193600 Rh2DG193800 Rh2DG334200 Rh2DG543100 Rh5AG280400 Rh5BG285800 Rh5BG285900 Rh5CG317900 Rh5CG318000 Rh5DG294600 Rh5DG294700 Rh6AG012200 Rh6AG012700 Rh6BG011200 Rh6BG011700
rosa_wichuraiana Rw0G009380 Rw2G014610 Rw2G014630 Rw2G014640 Rw2G014680 Rw2G025120 Rw2G043000 Rw5G026490 Rw6G001370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 85, 1792
Acc36I ACCTGC 4 cut(s) 85, 1092, 1286, 1792
AccB1I GGYRCC 1 cut(s) 1804
AccB7I CCANNNNNTGG 1 cut(s) 1240
AccI GTMKAC 1 cut(s) 1987
AccIII TCCGGA 1 cut(s) 590
AciI CCGC 2 cut(s) 92, 2113
AclWI GGATC 4 cut(s) 254, 1227, 1596, 1912
AcuI CTGAAG 5 cut(s) 892, 1243, 1784, 1929, 2036
AfaI GTAC 3 cut(s) 72, 1758, 2091
AfiI CCNNNNNNNGG 5 cut(s) 538, 971, 1088, 1240, 2290
AflII CTTAAG 1 cut(s) 1127
AjiI CACGTC 1 cut(s) 451
Alw21I GWGCWC 1 cut(s) 1871
Alw26I GTCTC 3 cut(s) 407, 1330, 1632
AlwI GGATC 4 cut(s) 254, 1227, 1596, 1912
Ama87I CYCGRG 2 cut(s) 275, 1808
Aor13HI TCCGGA 1 cut(s) 590
AoxI GGCC 3 cut(s) 724, 1555, 2292
ApeKI GCWGC 7 cut(s) 113, 116, 1140, 1143, 1528, 1935, 2298
Asp700I GAANNNNTTC 4 cut(s) 630, 656, 1389, 2279
AspS9I GGNCC 1 cut(s) 2292
AsuC2I CCSGG 5 cut(s) 533, 1089, 1253, 1809, 1810
AsuHPI GGTGA 6 cut(s) 200, 559, 926, 1171, 1313, 2284
AvaI CYCGRG 2 cut(s) 275, 1808
BaeGI GKGCMC 1 cut(s) 1809
BanI GGYRCC 1 cut(s) 1804
BanII GRGCYC 2 cut(s) 137, 1871
BarI GAAGNNNNNNTAC 3 cut(s) 2223, 2255, 2340
BauI CACGAG 2 cut(s) 491, 1167
BbsI GAAGAC 5 cut(s) 163, 198, 1592, 1686, 2063
Bbv12I GWGCWC 1 cut(s) 1871
BbvI GCAGC 7 cut(s) 125, 128, 1152, 1155, 1515, 1922, 2310
BccI CCATC 5 cut(s) 588, 851, 1210, 1409, 1502
BcgI CGANNNNNNTGC 2 cut(s) 1049, 1083
BciVI GTATCC 3 cut(s) 169, 256, 1156
BcnI CCSGG 5 cut(s) 533, 1089, 1253, 1809, 1810
BcoDI GTCTC 3 cut(s) 407, 1330, 1632
BfaI CTAG 5 cut(s) 68, 801, 1382, 1451, 2097
BfmI CTRYAG 4 cut(s) 645, 1043, 2266, 2296
BfrI CTTAAG 1 cut(s) 1127
BfuAI ACCTGC 4 cut(s) 85, 1092, 1286, 1792
BfuI GTATCC 3 cut(s) 169, 256, 1156
BglII AGATCT 1 cut(s) 301
BisI GCNGC 7 cut(s) 114, 117, 1141, 1144, 1529, 1936, 2299
BlsI GCNGC 7 cut(s) 115, 118, 1142, 1145, 1530, 1937, 2300
Bme1390I CCNGG 5 cut(s) 533, 1089, 1253, 1809, 1810
BmeT110I CYCGRG 2 cut(s) 275, 1808
BmgBI CACGTC 1 cut(s) 451
BmgT120I GGNCC 1 cut(s) 2292
BmiI GGNNCC 3 cut(s) 1806, 2162, 2293
BmrFI CCNGG 5 cut(s) 533, 1089, 1253, 1809, 1810
BmsI GCATC 6 cut(s) 247, 594, 805, 1020, 1705, 2007
BpiI GAAGAC 5 cut(s) 163, 198, 1592, 1686, 2063
BpmI CTGGAG 3 cut(s) 72, 660, 1220
Bpu10I CCTNAGC 1 cut(s) 1083
BpuEI CTTGAG 1 cut(s) 1570
BpuMI CCSGG 5 cut(s) 533, 1089, 1253, 1809, 1810
BsaJI CCNNGG 6 cut(s) 1251, 1789, 1808, 1809, 1830, 2284
BsaWI WCCGGW 2 cut(s) 590, 1859
BsaXI ACNNNNNCTCC 2 cut(s) 2073, 2103
Bsc4I CCNNNNNNNGG 5 cut(s) 538, 971, 1088, 1240, 2290
Bse1I ACTGG 1 cut(s) 437
BseAI TCCGGA 1 cut(s) 590
BseDI CCNNGG 6 cut(s) 1251, 1789, 1808, 1809, 1830, 2284
BseGI GGATG 9 cut(s) 105, 599, 604, 1035, 1445, 1799, 1972, 2031, 2108
BseLI CCNNNNNNNGG 5 cut(s) 538, 971, 1088, 1240, 2290
BseMII CTCAG 2 cut(s) 190, 1622
BseNI ACTGG 1 cut(s) 437
BseRI GAGGAG 2 cut(s) 398, 630
BseSI GKGCMC 1 cut(s) 1809
BseXI GCAGC 7 cut(s) 125, 128, 1152, 1155, 1515, 1922, 2310
BseYI CCCAGC 1 cut(s) 109
BshFI GGCC 3 cut(s) 726, 1557, 2294
BshNI GGYRCC 1 cut(s) 1804
BsiHKAI GWGCWC 1 cut(s) 1871
BsiHKCI CYCGRG 2 cut(s) 275, 1808
BsiSI CCGG 6 cut(s) 533, 591, 1088, 1253, 1809, 1860
BslFI GGGAC 3 cut(s) 297, 705, 2091
BslI CCNNNNNNNGG 5 cut(s) 538, 971, 1088, 1240, 2290
BsmAI GTCTC 3 cut(s) 407, 1330, 1632
BsmFI GGGAC 3 cut(s) 297, 705, 2091
BsmI GAATGC 3 cut(s) 254, 410, 1820
BsnI GGCC 3 cut(s) 726, 1557, 2294
BsoBI CYCGRG 2 cut(s) 275, 1808
Bsp1286I GDGCHC 3 cut(s) 137, 1809, 1871
Bsp13I TCCGGA 1 cut(s) 590
Bsp1407I TGTACA 1 cut(s) 2089
Bsp143I GATC 6 cut(s) 246, 301, 1219, 1588, 1900, 1917
Bsp19I CCATGG 1 cut(s) 1789
BspACI CCGC 2 cut(s) 92, 2113
BspANI GGCC 3 cut(s) 726, 1557, 2294
BspCNI CTCAG 2 cut(s) 189, 1623
BspEI TCCGGA 1 cut(s) 590
BspLI GGNNCC 3 cut(s) 1806, 2162, 2293
BspMAI CTGCAG 1 cut(s) 2300
BspMI ACCTGC 4 cut(s) 85, 1092, 1286, 1792
BspPI GGATC 4 cut(s) 254, 1227, 1596, 1912
BspT107I GGYRCC 1 cut(s) 1804
BspTI CTTAAG 1 cut(s) 1127
BsrGI TGTACA 1 cut(s) 2089
BsrI ACTGG 1 cut(s) 437
BssECI CCNNGG 6 cut(s) 1251, 1789, 1808, 1809, 1830, 2284
BssMI GATC 6 cut(s) 246, 301, 1219, 1588, 1900, 1917
BssNAI GTATAC 1 cut(s) 1988
BssSI CACGAG 2 cut(s) 491, 1167
BssT1I CCWWGG 2 cut(s) 1789, 1830
Bst1107I GTATAC 1 cut(s) 1988
Bst2BI CACGAG 2 cut(s) 491, 1167
Bst4CI ACNGT 4 cut(s) 206, 775, 929, 1284
Bst6I CTCTTC 4 cut(s) 45, 130, 405, 607
BstAFI CTTAAG 1 cut(s) 1127
BstAUI TGTACA 1 cut(s) 2089
BstC8I GCNNGC 9 cut(s) 390, 506, 583, 1409, 1582, 2048, 2193, 2197, 2296
BstDEI CTNAG 5 cut(s) 176, 1083, 1631, 1848, 2018
BstDSI CCRYGG 1 cut(s) 1789
BstF5I GGATG 9 cut(s) 105, 599, 604, 1035, 1445, 1799, 1972, 2031, 2108
BstKTI GATC 6 cut(s) 249, 304, 1222, 1591, 1903, 1920
BstMAI GTCTC 3 cut(s) 407, 1330, 1632
BstMBI GATC 6 cut(s) 246, 301, 1219, 1588, 1900, 1917
BstMWI GCNNNNNNNGC 5 cut(s) 359, 797, 1554, 1614, 1913
BstNSI RCATGY 1 cut(s) 903
BstSCI CCNGG 5 cut(s) 531, 1087, 1251, 1807, 1808
BstSFI CTRYAG 4 cut(s) 645, 1043, 2266, 2296
BstSLI GKGCMC 1 cut(s) 1809
BstV1I GCAGC 7 cut(s) 125, 128, 1152, 1155, 1515, 1922, 2310
BstV2I GAAGAC 5 cut(s) 163, 198, 1592, 1686, 2063
BstX2I RGATCY 3 cut(s) 301, 1219, 1588
BstXI CCANNNNNNTGG 1 cut(s) 1332
BstYI RGATCY 3 cut(s) 301, 1219, 1588
BstZ17I GTATAC 1 cut(s) 1988
BsuI GTATCC 3 cut(s) 169, 256, 1156
BsuRI GGCC 3 cut(s) 726, 1557, 2294
BtgI CCRYGG 1 cut(s) 1789
BtrI CACGTC 1 cut(s) 451
BtsCI GGATG 9 cut(s) 105, 599, 604, 1035, 1445, 1799, 1972, 2031, 2108
BtsI GCAGTG 2 cut(s) 727, 1725
BtsIMutI CAGTG 3 cut(s) 727, 1725, 1946
BveI ACCTGC 4 cut(s) 85, 1092, 1286, 1792
Cac8I GCNNGC 9 cut(s) 390, 506, 583, 1409, 1582, 2048, 2193, 2197, 2296
Cfr13I GGNCC 1 cut(s) 2292
Cfr9I CCCGGG 1 cut(s) 1808
CseI GACGC 3 cut(s) 260, 1367, 1631
Csp6I GTAC 3 cut(s) 71, 1757, 2090
CspCI CAANNNNNGTGG 2 cut(s) 2331, 2366
CviAII CATG 8 cut(s) 149, 164, 227, 372, 509, 900, 1034, 1790
CviQI GTAC 3 cut(s) 71, 1757, 2090
DdeI CTNAG 5 cut(s) 176, 1083, 1631, 1848, 2018
DpnI GATC 6 cut(s) 248, 303, 1221, 1590, 1902, 1919
DpnII GATC 6 cut(s) 246, 301, 1219, 1588, 1900, 1917
Eam1104I CTCTTC 4 cut(s) 45, 130, 405, 607
EarI CTCTTC 4 cut(s) 45, 130, 405, 607
Ecl136II GAGCTC 1 cut(s) 1869
Eco130I CCWWGG 2 cut(s) 1789, 1830
Eco24I GRGCYC 2 cut(s) 137, 1871
Eco32I GATATC 3 cut(s) 883, 970, 1637
Eco53kI GAGCTC 1 cut(s) 1869
Eco57I CTGAAG 5 cut(s) 892, 1243, 1784, 1929, 2036
Eco88I CYCGRG 2 cut(s) 275, 1808
EcoICRI GAGCTC 1 cut(s) 1869
EcoO109I RGGNCCY 1 cut(s) 2292
EcoRI GAATTC 1 cut(s) 1120
EcoRV GATATC 3 cut(s) 883, 970, 1637
EcoT14I CCWWGG 2 cut(s) 1789, 1830
EcoT22I ATGCAT 1 cut(s) 1035
EcoT38I GRGCYC 2 cut(s) 137, 1871
ErhI CCWWGG 2 cut(s) 1789, 1830
FaeI CATG 8 cut(s) 152, 167, 230, 375, 512, 903, 1037, 1793
FaqI GGGAC 3 cut(s) 297, 705, 2091
FatI CATG 8 cut(s) 148, 163, 226, 371, 508, 899, 1033, 1789
FauNDI CATATG 1 cut(s) 2141
FblI GTMKAC 1 cut(s) 1987
Fnu4HI GCNGC 7 cut(s) 114, 117, 1141, 1144, 1529, 1936, 2299
FokI GGATG 9 cut(s) 92, 606, 611, 1042, 1452, 1806, 1979, 2018, 2115
FriOI GRGCYC 2 cut(s) 137, 1871
Fsp4HI GCNGC 7 cut(s) 114, 117, 1141, 1144, 1529, 1936, 2299
FspBI CTAG 5 cut(s) 68, 801, 1382, 1451, 2097
GluI GCNGC 7 cut(s) 114, 117, 1141, 1144, 1529, 1936, 2299
GsaI CCCAGC 1 cut(s) 113
GsuI CTGGAG 3 cut(s) 72, 660, 1220
HaeIII GGCC 3 cut(s) 726, 1557, 2294
HapII CCGG 6 cut(s) 533, 591, 1088, 1253, 1809, 1860
HgaI GACGC 3 cut(s) 260, 1367, 1631
Hin1II CATG 8 cut(s) 152, 167, 230, 375, 512, 903, 1037, 1793
HincII GTYRAC 2 cut(s) 1246, 1287
HindII GTYRAC 2 cut(s) 1246, 1287
HindIII AAGCTT 5 cut(s) 564, 654, 1606, 1709, 2048
HinfI GANTC 9 cut(s) 95, 323, 620, 919, 1076, 1186, 1338, 1768, 1826
HpaII CCGG 6 cut(s) 533, 591, 1088, 1253, 1809, 1860
HphI GGTGA 6 cut(s) 200, 559, 926, 1171, 1313, 2284
Hpy166II GTNNAC 5 cut(s) 1246, 1287, 1321, 1988, 2204
Hpy8I GTNNAC 5 cut(s) 1246, 1287, 1321, 1988, 2204
Hpy99I CGWCG 2 cut(s) 254, 2169
HpyAV CCTTC 9 cut(s) 532, 538, 737, 818, 941, 1345, 1400, 1755, 1904
HpyCH4III ACNGT 4 cut(s) 206, 775, 929, 1284
HpyCH4IV ACGT 2 cut(s) 450, 2068
HpyF10VI GCNNNNNNNGC 5 cut(s) 359, 797, 1554, 1614, 1913
HpyF3I CTNAG 5 cut(s) 176, 1083, 1631, 1848, 2018
HpySE526I ACGT 2 cut(s) 450, 2068
Hsp92II CATG 8 cut(s) 152, 167, 230, 375, 512, 903, 1037, 1793
Kpn2I TCCGGA 1 cut(s) 590
Kzo9I GATC 6 cut(s) 246, 301, 1219, 1588, 1900, 1917
LmnI GCTCC 4 cut(s) 385, 679, 1689, 1913
Lsp1109I GCAGC 7 cut(s) 125, 128, 1152, 1155, 1515, 1922, 2310
LweI GCATC 6 cut(s) 247, 594, 805, 1020, 1705, 2007
MaeI CTAG 5 cut(s) 68, 801, 1382, 1451, 2097
MaeII ACGT 2 cut(s) 450, 2068
MaeIII GTNAC 6 cut(s) 200, 514, 1177, 1721, 2305, 2338
MalI GATC 6 cut(s) 248, 303, 1221, 1590, 1902, 1919
MboI GATC 6 cut(s) 246, 301, 1219, 1588, 1900, 1917
MfeI CAATTG 1 cut(s) 1841
MflI RGATCY 3 cut(s) 301, 1219, 1588
MhlI GDGCHC 3 cut(s) 137, 1809, 1871
MlyI GAGTC 2 cut(s) 928, 1332
MmeI TCCRAC 1 cut(s) 1944
Mph1103I ATGCAT 1 cut(s) 1035
MroI TCCGGA 1 cut(s) 590
MroXI GAANNNNTTC 4 cut(s) 630, 656, 1389, 2279
MspA1I CMGCKG 1 cut(s) 92
MspCI CTTAAG 1 cut(s) 1127
MspI CCGG 6 cut(s) 533, 591, 1088, 1253, 1809, 1860
MspR9I CCNGG 5 cut(s) 533, 1089, 1253, 1809, 1810
MunI CAATTG 1 cut(s) 1841
Mva1269I GAATGC 3 cut(s) 254, 410, 1820
MwoI GCNNNNNNNGC 5 cut(s) 359, 797, 1554, 1614, 1913
NciI CCSGG 5 cut(s) 533, 1089, 1253, 1809, 1810
NcoI CCATGG 1 cut(s) 1789
NdeI CATATG 1 cut(s) 2141
NdeII GATC 6 cut(s) 246, 301, 1219, 1588, 1900, 1917
NlaIII CATG 8 cut(s) 152, 167, 230, 375, 512, 903, 1037, 1793
NlaIV GGNNCC 3 cut(s) 1806, 2162, 2293
NmuCI GTSAC 5 cut(s) 200, 514, 1177, 1721, 2338
NsiI ATGCAT 1 cut(s) 1035
NspI RCATGY 1 cut(s) 903
PacI TTAATTAA 1 cut(s) 1005
PaeR7I CTCGAG 1 cut(s) 275
PaqCI CACCTGC 2 cut(s) 85, 1792
PcsI WCGNNNNNNNCGW 1 cut(s) 978
PctI GAATGC 3 cut(s) 254, 410, 1820
PdmI GAANNNNTTC 4 cut(s) 630, 656, 1389, 2279
PfeI GAWTC 7 cut(s) 95, 323, 620, 1076, 1186, 1768, 1826
PflMI CCANNNNNTGG 1 cut(s) 1240
PkrI GCNGC 7 cut(s) 115, 118, 1142, 1145, 1530, 1937, 2300
PleI GAGTC 2 cut(s) 927, 1332
PpsI GAGTC 2 cut(s) 927, 1332
Psp124BI GAGCTC 1 cut(s) 1871
PspFI CCCAGC 1 cut(s) 109
PspN4I GGNNCC 3 cut(s) 1806, 2162, 2293
PspPI GGNCC 1 cut(s) 2292
PstI CTGCAG 1 cut(s) 2300
PsuI RGATCY 3 cut(s) 301, 1219, 1588
RsaI GTAC 3 cut(s) 72, 1758, 2091
RsaNI GTAC 3 cut(s) 71, 1757, 2090
SacI GAGCTC 1 cut(s) 1871
SatI GCNGC 7 cut(s) 114, 117, 1141, 1144, 1529, 1936, 2299
Sau3AI GATC 6 cut(s) 246, 301, 1219, 1588, 1900, 1917
Sau96I GGNCC 1 cut(s) 2292
SchI GAGTC 2 cut(s) 928, 1332
ScrFI CCNGG 5 cut(s) 533, 1089, 1253, 1809, 1810
SduI GDGCHC 3 cut(s) 137, 1809, 1871
SfaNI GCATC 6 cut(s) 247, 594, 805, 1020, 1705, 2007
SfcI CTRYAG 4 cut(s) 645, 1043, 2266, 2296
Sfr274I CTCGAG 1 cut(s) 275
SlaI CTCGAG 1 cut(s) 275
SmaI CCCGGG 1 cut(s) 1810
SmlI CTYRAG 3 cut(s) 275, 1127, 1549
SmoI CTYRAG 3 cut(s) 275, 1127, 1549
SsiI CCGC 2 cut(s) 92, 2113
SspI AATATT 1 cut(s) 716
SspMI CTAG 5 cut(s) 68, 801, 1382, 1451, 2097
SstI GAGCTC 1 cut(s) 1871
StyD4I CCNGG 5 cut(s) 531, 1087, 1251, 1807, 1808
StyI CCWWGG 2 cut(s) 1789, 1830
TaaI ACNGT 4 cut(s) 206, 775, 929, 1284
TaiI ACGT 2 cut(s) 453, 2071
TaqI TCGA 4 cut(s) 249, 276, 844, 2217
TatI WGTACW 2 cut(s) 1756, 2089
TfiI GAWTC 7 cut(s) 95, 323, 620, 1076, 1186, 1768, 1826
TscAI CASTG 3 cut(s) 727, 1732, 1953
TseFI GTSAC 5 cut(s) 200, 514, 1177, 1721, 2338
TseI GCWGC 7 cut(s) 113, 116, 1140, 1143, 1528, 1935, 2298
Tsp45I GTSAC 5 cut(s) 200, 514, 1177, 1721, 2338
TspDTI ATGAA 9 cut(s) 248, 285, 525, 795, 825, 1600, 1839, 1983, 2302
TspGWI ACGGA 3 cut(s) 281, 970, 2153
TspMI CCCGGG 1 cut(s) 1808
TspRI CASTG 3 cut(s) 727, 1732, 1953
Van91I CCANNNNNTGG 1 cut(s) 1240
Vha464I CTTAAG 1 cut(s) 1127
XbaI TCTAGA 1 cut(s) 1381
XceI RCATGY 1 cut(s) 903
XhoI CTCGAG 1 cut(s) 275
XmaI CCCGGG 1 cut(s) 1808
XmiI GTMKAC 1 cut(s) 1987
XmnI GAANNNNTTC 4 cut(s) 630, 656, 1389, 2279
XspI CTAG 5 cut(s) 68, 801, 1382, 1451, 2097
Zsp2I ATGCAT 1 cut(s) 1035
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.