Rw2G014680

positive regulation of cell-substrate adhesion

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000307
Physical Location & Seq
Reverse (-)
16603180 .. 16630347
27168 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G014680.1

Sequence Viewer

Length: 7170 bp
ATGTACTTCGTCATCTGGCTCCTCTGTACTCTCTTTATTTCTTTTCTTCTTCGCTATCTCAGTTTTCTTCTAGGACGTAATCCTATAGCTGAGTTTCCAGCACCCGATCCCACCGAGACCCACAGAGACGTACCTTCATCATCTGGTTCATCCTCAAATTTGACACATCGGATTACTCTTCCAGAGGGTAATTTCCCCAACACCCAACTAAGACCATCATCTGCTTCTTCATCCTTTGTTTCGGAACCTAATCAGGAAGTTCCAACAAATTCCACCAATACACAGCTAGAACCTTCATCATCTATATCTTCTTCCCACTTTAATTGGGAATATGACGTCTTCCTCAGTTTTAGCGGCAAGGACACTCGCAATAGTTTTACAGACCATTTACACGATCGTATGAATGGATATGGAATTTCGACGTTCAGAGATACAGAAAACCTTCCCAAGGGAAGACCCATTTCAGAACTTTTAAGAGCAATTGAAGAATCACAGTCTGCCGTCGTAGTTCTTTCAACCAACTATGCTGGTTCAACTTGGTGTTTACGTGAACTAGCACATATTCTTAAGTGCAAGAAGCTGAGGGAAATGGAGAAGCTGAGGAGAATGGAAGTTCTTCCGGTTTTCTATAATGTAACACCGGCTGAGATAAAACATCAAATTGGAGACACTGCAGATCAAACTGGGGACTCTGTCGATCAGTCTGGAGACTCTGCCCATCCAAAGGGAGACTTTGCCAAGGCGTTTGCAAAGCACGAGAATCGTGTCAAGAAAAATAAGGAGAAGAAGGAGAATGTGGACGAGTGGAGGACAGCTTTGGATGAAGTTGCCGGTTTCTCCGGATGGCATGTAACGGGGCAAAGAAGTGAAAGAGATGTTATTCTGGAAATAGCTAATGTTGTTTCAAAAGCAGTCAGGTTACCCAGGCCTAAAAAATACTTGGTTGGAATGGAGGCACGTATAAAAGAAGTAAACTCCCGCTTGAATCTCGGGTCAAATGATGTTCTTACCATAGGGATTTGGGGAATGGGGGGAATCGGTAAGTCAACTCTTGCAGAGGAAGTATTCGAAAACATCCGTGAACAATTTCAGCATTGCGGCATTGTTTCTCGAGTTGGATTGCGACCCAAAGTTGAGGTGCAAAGACTTCTTTGTCAATCTTTTTCAGGGGATGGAAAGAATAATATCCATTGCGAGGAAAGAGGGATCAAGTTCTTGACGAAGGAACTTTTTCAGAAGAAGGTGCTTCTCGTTCTAGATGATGTTGATAATTTCAACCAGATAGAATGTCTAGCACCTGGAGGCCCGCTTGGACAGAACAACTGGGGTGGAGGGAGTCGACTCATTATAACCACCAGAGATAAGAGCACATTGAGAGGCTTTAATGTGGAAGAAAATGAAATATACGAGGTTGAAAAACTGGATGGTACGGAAGCTTTTGAGCTCTTACGTCACAAAGCCTTCCAATTCAAGAAAGACAATCCCCTAGATGAGTTGAAAAAGTGGTCTGATGAGTTTGAAAAGTGCTCTGGCAGTTTTCTGAAATATGCTGGCGGCTTTCCTTTAGCTCTTACAGTTCTGGGGTCATACTTATCTACAGAAGAGGTAGATGAATGGCCAGGGATATTGGATAGACTCGACAGTGACAAGGATCCAGACATTTTTAGTGTGCTTCAAATAAGTTATGATGGATTAAGGTTTAGAGCAGATAAGGAAATATTTTTGGACATTGCATGTTTCTTCAATGGTGAGAATCATGTCCGTGTGCAAAAGATATGGGAAGGTTGCGGATTTTGTTCCAACTCAAGTATAAGGAACCTTATTAACAAGTCCTTGATTAAAATTGGAAGCAATAATGAACTGTGGATGCATGATTTACTAAGGTGCTTGGGTTGGCATATTGTTCACGAAGAATCTCCTTTCATACCGAGGAATCGTAGCAGGTTATGGCTTGATGACAATGCACACAAGTACGAAGGCAGAATATCATGGCGTTTTGAGGATTCTCGGAATGTACTCGAGAAAAACAATGGAACAAGTGCTGTTAGAGGCTTATTCTTAAGCTTGCCCGAAAAGGAAGAAATGCGCATGATGAATGGTGACCCTTTGTTAAATATGCCCGACTTAAAATTGTTGAAGATTTGTAATGTTAACTTTCAGAATGTGGACTTTTCATATCTCCCTCCAGATTTACGGTTGTTGGACTGGCATGAATGTCCTCTAGAATCACTGCCATCTGATTTCAAATCGGACCAGCTGGTTGAATTTAAGATGCCTAACAGCCGCATTACACAACTATGGAATGAAGCGCTCTTTCTAAGAAGGCTAGTACTGATGGATCTTTCCAACTGCCAAGATTTAGCCAGGAGCCCTGACTTCAGTAAGGTCCCAAAGCTTGAGACATTGATCCTTGAAGGCTGCAAAAAGCTATCAAAGGTTCACGCAACATTTGGGGATCTCCAACATCTGGTTTTATTGAATTTGAAAGGTTGCGTGAGTCTAGATAGCCTACCCGCATCGATCAGCTTGAAATCTCTTGAAACTTTGATTCTTTCGGGATGTTCGAAAATCAAAGAGTTTCCAGAGATTTTGGGAGACATGAAAACTTTGTCTAAACTTTATCTAGATGGCACCGCTATACGAGAGCTGCCAGAATCAATCCGGCATTTGGAAGGCCTTACTTTGCTGAATCTAAGCCGCTGCAAGAAGCTTCTGAGTCTTCCGAACATCCTTTGTAGAAGTTTGACATCACTGAAATCTCTGCATATGTCAATGTGCTCAATTATGGACAAACTACCAGAGGACATAGGCTACTTGAAAAACTTGCAGGAGCTTGATGCGTGTGAGACTGCTATTAGAAAAGTGCCCGAGTCCATTTCACTACTCGAGAATCTTAAACTATTGTGTTTCCACGGATGCACGGGTTTGCAGTTGCCAAACTCGTTCTTAGGTCTATCATCTTTGACCACACTAAATCTAGGTGGATGCCGTTTAGCAGAGGGAGCGATTCCAGATGACATTGGCGACTTATTGTCATTGCAGAGTTTGGATTTGAGTGAAAACAATTTTTTTAGCATACCTGAAAGCATCTCTCAACTTTCTAAGCTTAAAGAAATGTCTTTGTTTGGGTGTGGCAAGCTACAGTGGTTGCCAAAAAATCCTCCGGAAAATCTAGAAAAGGTAGACCTGTGTGGTTGTTTGATAACTCCAGCTGTTTGGAAGAGATTGGTTTCAGGTAACGGTTTGAATATCATAAACTGTCAAAAACCACAGGAGGAAGAAGTGTTTCCTGATCCTCAACTAAATGAGTTTGCTTCAGAAGGACGGGAAGAACTCGATCTTTCGAACAGCCAACAGTTGACTCAGAGCCCAAACTTGATCAAGGTCCCAAATCTTAAGAGATTGATCCTTAAAGAATGTAAAAAATTAACAGAGGTGCACCCAACAGTGTGGGGTCTCCAACATCTGGTTTTATTGAATTTGAAAGGATGTGTAGATCTAAAGCTCCTTCCTCCCTCGATCAGATTGGGATCTCTTGAAGTTCTTATTCTTTCGGGATGTTCAAAACTTAAGAAATTTCCGGAGATCGATGGAGATATGAATAATTTATCAGAACTTCATTTAGATGGGACGGCGTTAAGCGGTCTTCCCACATCGCTGCAGCAATTGCAACGACTAGTTTTATTAAATCTAAGTGGCTGCAAGAATCTTTCGACCATTCCACACCTTTTGAGTCTGAAATCTCTCAATCTGTCTGGCTGCTCACTTTTTTCCAAATTGCCAATAAACCTGGGGAGCTGGACACGTTTGCAGGAGCTTAATGCCTCTGAAACTTCTTTAACAACTGTGCCTAGGTCAATTATATATCTCCGGAACCTCAAAGTGTTATCTTTCTATGGATGCATAGGATTGCAGCTGCAACATTGGTTTTCTGCGTTAAGCTCTTTAACATCCTTAAATCTAGGTAGCTGTGGTCTAGCAGAAGAAGTCTGGGATATCATCTGCCGCCTATCCTATTTGCAGCAATTGGATTTGAGTGGAAACAACTTTGAGAGATTACCAGATGAAATTGGTTGCCTATCCTCTTTGCAGCGATTGAATTTGAGTGGAAACAACTTTGTGAGTTTACCAGATGAAATTGGTTGCCTATCCTCTTTGCAGCGATTGAATTTGAGTGGAAACAACTTTGTGAGTTTACCAGATGAAATTGGTTGCCTATCCTCTTTGCAGCAATTGGATTTGAGTGGAAACAACTTCGTGGAGATACCTGAAAGCATCTCTCAACTTTCGGATCTTATAGAACTTGGCTTGTCTAGATGTAGTAAGCTACAGTCATTGCCAGAAAATCTTCCAGTCAATCTAAAACATCTCCATGCACGAGATTGCCCTGTGCTGGAAAAGTATGCAAATTTGACGATATCGGGGTCTGACAGAGGTTTCTGTTTCATAATTAACGAAAATTCAGACCAGGATGGTGATCATCATCCAAGGCACTTCCCACTTCCAGTTCCCAAAGAAGACCGTATTGAAGTTCTCTTTCCTAGATACATTGAGGATCGAATTTGTGGTAAAGAGCCATTTGAAATTCATTTTCCGCATAGTATAAGAATTCCCAATTTCTGGAGTTGTTGGACAAGTGGCCCTTTTGTGGAAATCCCAGTATACAACGGTACATGGATGGGATTTGCTCTGTTCGTTGTCTTTGAAATCCTTGAGAAAGACGATTTTGACAAGAGTTTAGAAGAAACTGTCTGTGATTTTTACACCAATAATGGTCATGACAAATCCCTAGTGTTTCAGAATTTCATAAACTTCAGGGTTGGGTCGTCATATGGAGTTTGTTGCTATGAACCACGAAGCGGGCAATTTGGTGGACTGTTAGATAACGTAAGCCCACTACTTGGAGCTTCAGTTTCGACCAAGAGACCCGATCTAATAGTGAGAGGGTGTGGGATACATCTAATATCCCAAGCTGAAGAGTTTGTTCAAAATTTAACATATCAGACTATTACTCAACACCAGGATTTCAATTTCGACCGACATTGTGAAGAAATATTAGATGAGACAACAACTGGTGATCTAATGGAACTAGGCCACGATCTACAGGAACTAGCAGGATCTCCTTCAACTGTGAATGAAGATAGCTGCAGCAAAAGTAACGCTATCATCCAACCCAGAGGAGAGCTGTCAATACTCTATAAGGGAGACAACGGTCGTAAGAAGTACTTTCATTTTTGTTTTCCTGCTCCTATTCTTCCAACTCCTCCCTGGTTCCTCCATCACCACGCAGGGGATGTAACATTGTGTTACACCACTAAAAACTTATTTGATGATGAGAGATGGGTGGGATTAGAACTATGCGTTCAATTTTCTCGGTCTACTTCTACTTCGACATCTGGCAATAATAGTTTTTTCTTTTATGTTGATTTGTGCTCTCACAATCATGAAAGTGAAGTAATGCACGGATCCCTCAAGATAAACAGTTGTGTTGGGACCTCAGATCAACTTGTTGTATTACATGTGCCACGTGTTCATTTTCAACAACAGTTGAATCAATGTCAGGGCATTAGCGCATTGTTCAGAACCACTGATGCAGAAATGGAGGTCCTAGTGTGTGGAAGCCGTTTAGCTTTCGACCAAGATTTGGAAGACTTGACCCATTCATTAACTGCGGGTGCTGGCAAGTTGGGGCAACATAGATGCAGAAGTACTGCACTAGTTCTAACAGAACAAGCTCCTTCTTCTTCAATTGTGCGGCTGCGAAATCATGGATGCTATCTCCAGCAACAGCGAGGTTTTGGGGAGTCTGACCTTACCCAATTGGTCCACTTCAGATCAGTAATGTTATTCCACTCCAGAAGCCTATTGGAAAACAGGGATAGAAACCAAGAAAACGTTGCCGAAGAAGATAAATCGTTGCTGTTGGCTCATCATCGTATACGTATAATGGCTGAGACTCTTATTTACGGAAGCTATAGTCCACCACGGTGGAAGAGATGCCTTCACTTGCTGCTTCGACACTTCAAGGAGGTCACTCTCAGTCTTGGTGGACATACAATTTCTGCTCTCAAGAAATTTGATCCTTCCTCCGCATGGAATAATATTAATTTCACTGCCACGAAAATTCCAGTGTGGTTCAAAGATAAGAGGGCTAACGTGTCTTCTATGTCCAGGGTGGGAATCAAGCTACCTTCAAAATTGCACGAGGATAAGAATTGGCGAGGACTTGTTATATGTGTTGCCTTTAAAGTTCATGACCAGCGTCCAACTACCTCCCTGGTCAAACTTCTCTGTCACTTGAGAGCCAAGGACAGCTATTGCTTGAATCCTATCCCTATGTGTTCAATCACTGAAGAGAAACTCAAGTCGTTGCATGTTGATAGTTTCATTTGGCTAAGCTACATCCCCCGTGTTTTGCTAACGGAGTTCAATGTGGTTAGTGATGTAGAGGCCAGAATTTATGTTGGTTGCAAAGGCTTGACAGTGGAGAAGTGTGGTATGCGTCTCTTGTACCGGCAAGAAGAGGGGGAGTTTGAGAGCGCGATAACTGAGTGGTGGACCCCTTTCTTTGGTAATCTACCTAATTTCATCCGTCGGCTGGTGGAAGCAGAACGTATCAAGGTCTTGGATCCGGATTTAATATATAATGCAATTCCCCCTTCTACTGGAATTCTGGAGTGGTTTGAGGCTGACATTAAGGACTGCCTAGCTGGCTCGAGCAGTAGCCAGAGCTGGGAATTGAAGTTACCACCATCTTCGAGCGACAAAAATTGGGAAGGACTGGCTCTCTGTGTATCATTTGTTCCTTACAAATCGCCTCAGGGAAACAAAGAATTCAATTTGAAGTTGGAAACTAGAGACAGTGGTTTGTCATATGTCCATCGGTATCAAATGAGCAATAAAGAATATGAGTTCGTGGAGGGCTCGATACGTCGTGAAAGAGCGCTACGTCTTGAAAACTATACGCTTCCTTACATACAATTAATTTGGCTCTCTTACATACCGCGACGCTTGTTTCTAGATCAGCTAAATGATGAGCCTGTCCTCATTGCTTCACCTGACCACTCGTGGTTCGTGGTCGAGGTCTATCACCGATTTGTATATGCGCATGACGTTGAAGAGTTTAATCAGCTCCGTATCGACCTTCATCAACCCGGTGCCCAACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04064 ko04115 ko04210 map04064 map04115 map04210
Pfam Domains
Protein Families
Death LRR_8 Peptidase_S68 ZU5

Protein Analysis

2389

Amino Acids

270.28

Weight (kDa)

5.92

Isoelectric Point (pI)

42.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 111 - 227 1.4e-29 TIR domain
TIR_2 PF13676 113 - 214 2.7e-12 TIR domain
NB-ARC PF00931 318 - 491 1.2e-16 NB-ARC domain
WHD_ROQ1 PF23282 570 - 639 5.9e-16 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 790 - 900 3.5e-08 Leucine-rich repeat region
LRR_13 PF23286 843 - 911 3e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 855 - 925 9.1e-07 Leucine-rich repeat region
LRR_14 PF23598 894 - 993 6.1e-11 Leucine-rich repeat region
LRR_14 PF23598 1218 - 1322 5.3e-08 Leucine-rich repeat region
LRR_8 PF13855 1315 - 1373 2.9e-06 Leucine rich repeat
LRR_14 PF23598 1319 - 1392 9.3e-09 Leucine-rich repeat region
LRR_8 PF13855 1362 - 1419 2e-09 Leucine rich repeat
LRR_14 PF23598 1379 - 1450 1.5e-10 Leucine-rich repeat region
LRR_8 PF13855 1385 - 1440 2.9e-06 Leucine rich repeat
C-JID PF20160 2039 - 2174 4.3e-06 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000307)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g16600 FvH4_1g16610 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16621 FvH4_1g16630 FvH4_1g16640 FvH4_1g16640 FvH4_1g16650 FvH4_1g16650 FvH4_3g27631 FvH4_4g11392 FvH4_4g11542
prunus_persica Prupe.4G224500_v2.0.a1 Prupe.4G226900_v2.0.a1 Prupe.4G227000_v2.0.a1
pyrus_communis pycom11g18180 pycom11g18210 pycom11g18230 pycom11g18270 pycom11g18350 pycom11g18360 pycom11g18370
rosa_chinensis RchiOBHm_Chr2g0106511 RchiOBHm_Chr2g0106541 RchiOBHm_Chr2g0106551 RchiOBHm_Chr2g0106571 RchiOBHm_Chr2g0124621 RchiOBHm_Chr6g0251591 RchiOBHm_Chr6g0251631 RchiOBHm_Chr6g0251641
rosa_laevigata RLG00000015443 RLG00000015444 RLG00000015458 RLG00000017510 RLG00000017511 RLG00000017512 RLG00000017515 RLG00000017516 RLG00000017517 RLG00000018809 RLG00000020934 RLG00000020935 RLG00000034092
rosa_multiflora Rmu_co8285777.1_g000001 Rmu_sc0000762.1_g000001 Rmu_sc0001872.1_g000006 Rmu_sc0005255.1_g000019 Rmu_sc0005597.1_g000006 Rmu_sc0012104.1_g000001 Rmu_sc0017105.1_g000011 Rmu_sc0022904.1_g000001 Rmu_ssc0000135.1_g000049 Rmu_ssc0000213.1_g000047
rosa_roxburghii Rroxscaffold_2G00092830 Rroxscaffold_2G00119790 Rroxscaffold_2G00137010 Rroxscaffold_2G00137030 Rroxscaffold_2G00137070 Rroxscaffold_7G00216560
rosa_rugosa Rorug02G0136100 Rorug02G0136200 Rorug02G0136300 Rorug02G0136400 Rorug02G0251800 Rorug02G0251800 Rorug02G0455500 Rorug02G0455600 Rorug02G0455700 Rorug05G0195300
rosa_samantha Rh2AG187400 Rh2AG187500 Rh2AG187600 Rh2AG310500 Rh2BG198200 Rh2BG198300 Rh2BG198400 Rh2BG198600 Rh2BG318900 Rh2BG534300 Rh2CG192400 Rh2CG192500 Rh2CG192600 Rh2CG297500 Rh2DG193500 Rh2DG193600 Rh2DG193800 Rh2DG334200 Rh2DG543100 Rh5AG280400 Rh5BG285800 Rh5BG285900 Rh5CG317900 Rh5CG318000 Rh5DG294600 Rh5DG294700 Rh6AG012200 Rh6AG012700 Rh6BG011200 Rh6BG011700
rosa_wichuraiana Rw0G009380 Rw2G014610 Rw2G014630 Rw2G014640 Rw2G014680 Rw2G025120 Rw2G043000 Rw5G026490 Rw6G001370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1349
AasI GACNNNNNNGTC 1 cut(s) 1152
AatII GACGTC 1 cut(s) 339
Acc16I TGCGCA 2 cut(s) 2087, 7107
Acc36I ACCTGC 1 cut(s) 1932
AccB1I GGYRCC 2 cut(s) 2630, 7157
AccB7I CCANNNNNTGG 5 cut(s) 2467, 3466, 4620, 4901, 5644
AccI GTMKAC 5 cut(s) 1339, 3183, 4662, 5378, 5938
AccII CGCG 2 cut(s) 6542, 7006
AccIII TCCGGA 5 cut(s) 839, 3163, 3580, 3870, 6631
AclI AACGTT 1 cut(s) 5895
AcoI YGGCCR 1 cut(s) 1616
AcuI CTGAAG 7 cut(s) 2362, 3300, 4798, 4893, 4995, 5815, 6372
AcvI CACGTG 1 cut(s) 5528
AcyI GRCGYC 1 cut(s) 336
AfeI AGCGCT 2 cut(s) 2310, 6945
AflII CTTAAG 4 cut(s) 566, 2059, 3395, 3569
AflIII ACRYGT 4 cut(s) 3803, 5518, 5527, 6156
AhdI GACNNNNNGTC 1 cut(s) 3031
AhlI ACTAGT 2 cut(s) 3676, 5716
AleI CACNNNNGTG 1 cut(s) 5986
Alw21I GWGCWC 6 cut(s) 1370, 1446, 1529, 2780, 3441, 5435
Alw44I GTGCAC 1 cut(s) 3437
Ama87I CYCGRG 6 cut(s) 989, 1110, 2018, 2867, 2885, 6715
Aor13HI TCCGGA 5 cut(s) 839, 3163, 3580, 3870, 6631
Aor51HI AGCGCT 2 cut(s) 2310, 6945
AoxI GGCC 7 cut(s) 926, 1303, 1616, 2674, 4639, 5092, 6450
ApaLI GTGCAC 1 cut(s) 3437
AseI ATTAAT 2 cut(s) 6105, 6983
Asp700I GAANNNNTTC 6 cut(s) 441, 615, 1086, 1230, 3285, 4347
AspA2I CCTAGG 1 cut(s) 3851
AspLEI GCGC 6 cut(s) 2088, 2311, 5573, 6542, 6946, 7108
AspS9I GGNCC 9 cut(s) 1304, 2251, 2386, 3385, 4640, 5493, 5605, 5824, 6558
AsuC2I CCSGG 1 cut(s) 7155
AsuHPI GGTGA 7 cut(s) 1760, 2111, 4487, 5087, 5273, 7047, 7082
AsuII TTCGAA 3 cut(s) 1068, 2564, 3344
AvaI CYCGRG 6 cut(s) 989, 1110, 2018, 2867, 2885, 6715
AvaII GGWCC 7 cut(s) 2251, 2386, 3385, 5493, 5605, 5824, 6558
AvrII CCTAGG 1 cut(s) 3851
AxyI CCTNAGG 1 cut(s) 6819
BaeGI GKGCMC 3 cut(s) 2868, 3441, 7162
BalI TGGCCA 1 cut(s) 1618
BamHI GGATCC 3 cut(s) 1651, 5465, 6628
BanI GGYRCC 2 cut(s) 2630, 7157
BanII GRGCYC 4 cut(s) 1446, 2372, 3371, 6926
BauI CACGAG 4 cut(s) 755, 4377, 6203, 7066
BbrPI CACGTG 1 cut(s) 5528
BbsI GAAGAC 7 cut(s) 331, 460, 2711, 3638, 4524, 5655, 6153
Bbv12I GWGCWC 6 cut(s) 1370, 1446, 1529, 2780, 3441, 5435
BbvCI CCTCAGC 2 cut(s) 581, 599
BceAI ACGGC 4 cut(s) 485, 2973, 3648, 5607
BcgI CGANNNNNNTGC 2 cut(s) 743, 777
BciVI GTATCC 1 cut(s) 4947
BclI TGATCA 2 cut(s) 3378, 4477
BcnI CCSGG 1 cut(s) 7155
BcuI ACTAGT 2 cut(s) 3676, 5716
BfmI CTRYAG 9 cut(s) 84, 672, 1596, 3140, 3659, 4328, 5102, 5146, 5974
BfoI RGCGCY 2 cut(s) 2312, 6947
BfrI CTTAAG 4 cut(s) 566, 2059, 3395, 3569
BfuAI ACCTGC 1 cut(s) 1932
BfuI GTATCC 1 cut(s) 4947
BglI GCCNNNNNGGC 1 cut(s) 6711
BglII AGATCT 1 cut(s) 3496
BlnI CCTAGG 1 cut(s) 3851
BlpI GCTNAGC 1 cut(s) 6395
BmcAI AGTACT 3 cut(s) 2331, 5225, 5710
Bme18I GGWCC 7 cut(s) 2251, 2386, 3385, 5493, 5605, 5824, 6558
BmeRI GACNNNNNGTC 1 cut(s) 3031
BmeT110I CYCGRG 6 cut(s) 989, 1110, 2018, 2867, 2885, 6715
BmgT120I GGNCC 9 cut(s) 1304, 2251, 2386, 3385, 4640, 5493, 5605, 5824, 6558
BmrI ACTGGG 3 cut(s) 693, 1333, 4652
BmuI ACTGGG 3 cut(s) 693, 1333, 4652
BoxI GACNNNNGTC 2 cut(s) 5211, 6261
BpiI GAAGAC 7 cut(s) 331, 460, 2711, 3638, 4524, 5655, 6153
BpmI CTGGAG 8 cut(s) 726, 1320, 2169, 3192, 4642, 5765, 5839, 6695
Bpu10I CCTNAGC 2 cut(s) 581, 599
Bpu1102I GCTNAGC 1 cut(s) 6395
Bpu14I TTCGAA 3 cut(s) 1068, 2564, 3344
BpuEI CTTGAG 7 cut(s) 1789, 2417, 4733, 5456, 6053, 6319, 6347
BpuMI CCSGG 1 cut(s) 7155
Bsa29I ATCGAT 2 cut(s) 2519, 3588
BsaAI YACGTR 4 cut(s) 548, 959, 5528, 5942
BsaBI GATNNNNATC 3 cut(s) 3529, 4476, 4482
BsaHI GRCGYC 1 cut(s) 336
BsaI GGTCTC 3 cut(s) 110, 3461, 4918
BsaWI WCCGGW 6 cut(s) 619, 839, 3163, 3580, 3870, 6631
BsaXI ACNNNNNCTCC 2 cut(s) 4896, 4926
Bse118I RCCGGY 3 cut(s) 640, 830, 6513
Bse21I CCTNAGG 1 cut(s) 6819
Bse3DI GCAATG 6 cut(s) 1093, 1189, 1728, 3035, 4334, 7047
Bse8I GATNNNNATC 3 cut(s) 3529, 4476, 4482
BseAI TCCGGA 5 cut(s) 839, 3163, 3580, 3870, 6631
BseCI ATCGAT 2 cut(s) 2519, 3588
BseJI GATNNNNATC 3 cut(s) 3529, 4476, 4482
BseMI GCAATG 6 cut(s) 1093, 1189, 1728, 3035, 4334, 7047
BseRI GAGGAG 4 cut(s) 11, 616, 5193, 5253
BseSI GKGCMC 3 cut(s) 2868, 3441, 7162
BseYI CCCAGC 1 cut(s) 6732
BsgI GTGCAG 1 cut(s) 5697
Bsh1236I CGCG 2 cut(s) 6542, 7006
Bsh1285I CGRYCG 3 cut(s) 397, 5038, 5215
BshFI GGCC 7 cut(s) 928, 1305, 1618, 2676, 4641, 5094, 6452
BshNI GGYRCC 2 cut(s) 2630, 7157
BshVI ATCGAT 2 cut(s) 2519, 3588
BsiEI CGRYCG 3 cut(s) 397, 5038, 5215
BsiHKAI GWGCWC 6 cut(s) 1370, 1446, 1529, 2780, 3441, 5435
BsiHKCI CYCGRG 6 cut(s) 989, 1110, 2018, 2867, 2885, 6715
BslFI GGGAC 5 cut(s) 701, 2372, 3371, 3643, 5506
BsmBI CGTCTC 2 cut(s) 120, 6509
BsmFI GGGAC 5 cut(s) 701, 2372, 3371, 3643, 5506
BsnI GGCC 7 cut(s) 928, 1305, 1618, 2676, 4641, 5094, 6452
Bso31I GGTCTC 3 cut(s) 110, 3461, 4918
BsoBI CYCGRG 6 cut(s) 989, 1110, 2018, 2867, 2885, 6715
Bsp119I TTCGAA 3 cut(s) 1068, 2564, 3344
Bsp13I TCCGGA 5 cut(s) 839, 3163, 3580, 3870, 6631
Bsp1720I GCTNAGC 1 cut(s) 6395
BspANI GGCC 7 cut(s) 928, 1305, 1618, 2676, 4641, 5094, 6452
BspDI ATCGAT 2 cut(s) 2519, 3588
BspEI TCCGGA 5 cut(s) 839, 3163, 3580, 3870, 6631
BspFNI CGCG 2 cut(s) 6542, 7006
BspHI TCATGA 3 cut(s) 4777, 5443, 6253
BspMAI CTGCAG 3 cut(s) 676, 3663, 5150
BspMI ACCTGC 1 cut(s) 1932
BspT104I TTCGAA 3 cut(s) 1068, 2564, 3344
BspT107I GGYRCC 2 cut(s) 2630, 7157
BspTI CTTAAG 4 cut(s) 566, 2059, 3395, 3569
BspTNI GGTCTC 3 cut(s) 110, 3461, 4918
BsrDI GCAATG 6 cut(s) 1093, 1189, 1728, 3035, 4334, 7047
BsrFI RCCGGY 3 cut(s) 640, 830, 6513
BssAI RCCGGY 3 cut(s) 640, 830, 6513
BssNAI GTATAC 2 cut(s) 4663, 5939
BssNI GRCGYC 1 cut(s) 336
BssSI CACGAG 4 cut(s) 755, 4377, 6203, 7066
BssT1I CCWWGG 5 cut(s) 447, 738, 3851, 4487, 6306
Bst1107I GTATAC 2 cut(s) 4663, 5939
Bst2BI CACGAG 4 cut(s) 755, 4377, 6203, 7066
Bst6I CTCTTC 8 cut(s) 183, 1596, 3215, 4971, 5987, 6348, 6516, 7113
BstACI GRCGYC 1 cut(s) 336
BstAFI CTTAAG 4 cut(s) 566, 2059, 3395, 3569
BstAPI GCANNNNNTGC 1 cut(s) 3667
BstBAI YACGTR 4 cut(s) 548, 959, 5528, 5942
BstBI TTCGAA 3 cut(s) 1068, 2564, 3344
BstC8I GCNNGC 7 cut(s) 1307, 1552, 2066, 3137, 4862, 5680, 6712
BstDSI CCRYGG 2 cut(s) 2911, 5984
BstEII GGTNACC 2 cut(s) 918, 2099
BstENI CCTNNNNNAGG 1 cut(s) 446
BstFNI CGCG 2 cut(s) 6542, 7006
BstH2I RGCGCY 2 cut(s) 2312, 6947
BstHHI GCGC 6 cut(s) 2088, 2311, 5573, 6542, 6946, 7108
BstMCI CGRYCG 3 cut(s) 397, 5038, 5215
BstMWI GCNNNNNNNGC 3 cut(s) 3002, 3667, 6711
BstNSI RCATGY 4 cut(s) 851, 1737, 5522, 6377
BstPAI GACNNNNGTC 2 cut(s) 5211, 6261
BstPI GGTNACC 2 cut(s) 918, 2099
BstSFI CTRYAG 9 cut(s) 84, 672, 1596, 3140, 3659, 4328, 5102, 5146, 5974
BstSLI GKGCMC 3 cut(s) 2868, 3441, 7162
BstSNI TACGTA 1 cut(s) 5942
BstUI CGCG 2 cut(s) 6542, 7006
BstV2I GAAGAC 7 cut(s) 331, 460, 2711, 3638, 4524, 5655, 6153
BstX2I RGATCY 9 cut(s) 1651, 2338, 2455, 3496, 3530, 4291, 5117, 5465, 6628
BstXI CCANNNNNNTGG 3 cut(s) 3216, 3450, 5988
BstYI RGATCY 9 cut(s) 1651, 2338, 2455, 3496, 3530, 4291, 5117, 5465, 6628
BstZ17I GTATAC 2 cut(s) 4663, 5939
Bsu15I ATCGAT 2 cut(s) 2519, 3588
Bsu36I CCTNAGG 1 cut(s) 6819
BsuI GTATCC 1 cut(s) 4947
BsuRI GGCC 7 cut(s) 928, 1305, 1618, 2676, 4641, 5094, 6452
BsuTUI ATCGAT 2 cut(s) 2519, 3588
BtgI CCRYGG 2 cut(s) 2911, 5984
BtgZI GCGATG 1 cut(s) 3639
BtsI GCAGTG 3 cut(s) 669, 2228, 6111
BveI ACCTGC 1 cut(s) 1932
Cac8I GCNNGC 7 cut(s) 1307, 1552, 2066, 3137, 4862, 5680, 6712
CciI TCATGA 3 cut(s) 4777, 5443, 6253
CfoI GCGC 6 cut(s) 2088, 2311, 5573, 6542, 6946, 7108
Cfr10I RCCGGY 3 cut(s) 640, 830, 6513
Cfr13I GGNCC 9 cut(s) 1304, 2251, 2386, 3385, 4640, 5493, 5605, 5824, 6558
ClaI ATCGAT 2 cut(s) 2519, 3588
CseI GACGC 3 cut(s) 6251, 6491, 7017
CspCI CAANNNNNGTGG 6 cut(s) 305, 340, 1309, 1344, 3711, 3746
DraI TTTAAA 1 cut(s) 6247
DrdI GACNNNNNNGTC 1 cut(s) 1152
DriI GACNNNNNGTC 1 cut(s) 3031
DseDI GACNNNNNNGTC 1 cut(s) 1152
EaeI YGGCCR 1 cut(s) 1616
Eam1104I CTCTTC 8 cut(s) 183, 1596, 3215, 4971, 5987, 6348, 6516, 7113
Eam1105I GACNNNNNGTC 1 cut(s) 3031
EarI CTCTTC 8 cut(s) 183, 1596, 3215, 4971, 5987, 6348, 6516, 7113
Ecl136II GAGCTC 1 cut(s) 1444
Eco105I TACGTA 1 cut(s) 5942
Eco130I CCWWGG 5 cut(s) 447, 738, 3851, 4487, 6306
Eco147I AGGCCT 2 cut(s) 928, 2676
Eco24I GRGCYC 4 cut(s) 1446, 2372, 3371, 6926
Eco31I GGTCTC 3 cut(s) 110, 3461, 4918
Eco32I GATATC 2 cut(s) 3997, 4419
Eco47I GGWCC 7 cut(s) 2251, 2386, 3385, 5493, 5605, 5824, 6558
Eco47III AGCGCT 2 cut(s) 2310, 6945
Eco53kI GAGCTC 1 cut(s) 1444
Eco57I CTGAAG 7 cut(s) 2362, 3300, 4798, 4893, 4995, 5815, 6372
Eco72I CACGTG 1 cut(s) 5528
Eco81I CCTNAGG 1 cut(s) 6819
Eco88I CYCGRG 6 cut(s) 989, 1110, 2018, 2867, 2885, 6715
Eco91I GGTNACC 2 cut(s) 918, 2099
EcoICRI GAGCTC 1 cut(s) 1444
EcoNI CCTNNNNNAGG 1 cut(s) 446
EcoO109I RGGNCCY 4 cut(s) 2386, 3385, 5493, 5605
EcoO65I GGTNACC 2 cut(s) 918, 2099
EcoRI GAATTC 3 cut(s) 4608, 6669, 6833
EcoRV GATATC 2 cut(s) 3997, 4419
EcoT14I CCWWGG 5 cut(s) 447, 738, 3851, 4487, 6306
EcoT22I ATGCAT 2 cut(s) 1872, 3905
EcoT38I GRGCYC 4 cut(s) 1446, 2372, 3371, 6926
ErhI CCWWGG 5 cut(s) 447, 738, 3851, 4487, 6306
Esp3I CGTCTC 2 cut(s) 120, 6509
FalI AAGNNNNNCTT 6 cut(s) 716, 748, 4627, 4659, 5210, 5242
FaqI GGGAC 5 cut(s) 701, 2372, 3371, 3643, 5506
FauI CCCGC 5 cut(s) 986, 1314, 2521, 4853, 5665
FauNDI CATATG 3 cut(s) 2766, 4831, 6874
FbaI TGATCA 2 cut(s) 3378, 4477
FblI GTMKAC 5 cut(s) 1339, 3183, 4662, 5378, 5938
FriOI GRGCYC 4 cut(s) 1446, 2372, 3371, 6926
FspAI RTGCGCAY 2 cut(s) 2087, 7107
FspI TGCGCA 2 cut(s) 2087, 7107
GlaI GCGC 6 cut(s) 2087, 2310, 5572, 6541, 6945, 7107
GsaI CCCAGC 1 cut(s) 6736
GsuI CTGGAG 8 cut(s) 726, 1320, 2169, 3192, 4642, 5765, 5839, 6695
HaeII RGCGCY 2 cut(s) 2312, 6947
HaeIII GGCC 7 cut(s) 928, 1305, 1618, 2676, 4641, 5094, 6452
HgaI GACGC 3 cut(s) 6251, 6491, 7017
HhaI GCGC 6 cut(s) 2088, 2311, 5573, 6542, 6946, 7108
Hin1I GRCGYC 1 cut(s) 336
Hin6I GCGC 6 cut(s) 2086, 2309, 5571, 6540, 6944, 7106
HinP1I GCGC 6 cut(s) 2086, 2309, 5571, 6540, 6944, 7106
HincII GTYRAC 4 cut(s) 1047, 1340, 2152, 3360
HindII GTYRAC 4 cut(s) 1047, 1340, 2152, 3360
HindIII AAGCTT 5 cut(s) 1434, 2062, 2393, 2708, 3104
HpaI GTTAAC 1 cut(s) 2152
HphI GGTGA 7 cut(s) 1760, 2111, 4487, 5087, 5273, 7047, 7082
Hpy99I CGWCG 5 cut(s) 424, 506, 6597, 6936, 7011
HpyF10VI GCNNNNNNNGC 3 cut(s) 3002, 3667, 6711
Hsp92I GRCGYC 1 cut(s) 336
HspAI GCGC 6 cut(s) 2086, 2309, 5571, 6540, 6944, 7106
Kpn2I TCCGGA 5 cut(s) 839, 3163, 3580, 3870, 6631
Ksp22I TGATCA 2 cut(s) 3378, 4477
KspAI GTTAAC 1 cut(s) 2152
MfeI CAATTG 6 cut(s) 480, 3665, 4025, 4232, 5748, 5819
MflI RGATCY 9 cut(s) 1651, 2338, 2455, 3496, 3530, 4291, 5117, 5465, 6628
MlsI TGGCCA 1 cut(s) 1618
MluNI TGGCCA 1 cut(s) 1618
Mox20I TGGCCA 1 cut(s) 1618
Mph1103I ATGCAT 2 cut(s) 1872, 3905
MroI TCCGGA 5 cut(s) 839, 3163, 3580, 3870, 6631
MroXI GAANNNNTTC 6 cut(s) 441, 615, 1086, 1230, 3285, 4347
MscI TGGCCA 1 cut(s) 1618
MslI CAYNNNNRTG 7 cut(s) 1761, 2296, 3624, 4371, 5442, 5448, 5986
Msp20I TGGCCA 1 cut(s) 1618
MspA1I CMGCKG 4 cut(s) 2257, 2700, 3212, 3916
MspCI CTTAAG 4 cut(s) 566, 2059, 3395, 3569
MunI CAATTG 6 cut(s) 480, 3665, 4025, 4232, 5748, 5819
MvnI CGCG 2 cut(s) 6542, 7006
MwoI GCNNNNNNNGC 3 cut(s) 3002, 3667, 6711
NciI CCSGG 1 cut(s) 7155
NdeI CATATG 3 cut(s) 2766, 4831, 6874
NmuCI GTSAC 5 cut(s) 1451, 1643, 2099, 6031, 6293
NsbI TGCGCA 2 cut(s) 2087, 7107
NsiI ATGCAT 2 cut(s) 1872, 3905
NspI RCATGY 4 cut(s) 851, 1737, 5522, 6377
NspV TTCGAA 3 cut(s) 1068, 2564, 3344
OliI CACNNNNGTG 1 cut(s) 5986
PaeR7I CTCGAG 4 cut(s) 1110, 2018, 2885, 6715
PagI TCATGA 3 cut(s) 4777, 5443, 6253
PceI AGGCCT 2 cut(s) 928, 2676
PciI ACATGT 1 cut(s) 5518
PcsI WCGNNNNNNNCGW 1 cut(s) 2561
PdmI GAANNNNTTC 6 cut(s) 441, 615, 1086, 1230, 3285, 4347
PflFI GACNNNGTC 1 cut(s) 692
PflMI CCANNNNNTGG 5 cut(s) 2467, 3466, 4620, 4901, 5644
Ple19I CGATCG 1 cut(s) 397
PmaCI CACGTG 1 cut(s) 5528
PmlI CACGTG 1 cut(s) 5528
Ppu21I YACGTR 4 cut(s) 548, 959, 5528, 5942
PpuMI RGGWCCY 4 cut(s) 2386, 3385, 5493, 5605
PscI ACATGT 1 cut(s) 5518
PshAI GACNNNNGTC 2 cut(s) 5211, 6261
PshBI ATTAAT 2 cut(s) 6105, 6983
PsiI TTATAA 1 cut(s) 1349
Psp124BI GAGCTC 1 cut(s) 1446
Psp1406I AACGTT 1 cut(s) 5895
Psp5II RGGWCCY 4 cut(s) 2386, 3385, 5493, 5605
PspCI CACGTG 1 cut(s) 5528
PspEI GGTNACC 2 cut(s) 918, 2099
PspFI CCCAGC 1 cut(s) 6732
PspPI GGNCC 9 cut(s) 1304, 2251, 2386, 3385, 4640, 5493, 5605, 5824, 6558
PspPPI RGGWCCY 4 cut(s) 2386, 3385, 5493, 5605
PspXI VCTCGAGB 1 cut(s) 6715
PsrI GAACNNNNNNTAC 2 cut(s) 6786, 6818
PstI CTGCAG 3 cut(s) 676, 3663, 5150
PsuI RGATCY 9 cut(s) 1651, 2338, 2455, 3496, 3530, 4291, 5117, 5465, 6628
PsyI GACNNNGTC 1 cut(s) 692
PvuI CGATCG 1 cut(s) 397
PvuII CAGCTG 3 cut(s) 2257, 3212, 3916
RseI CAYNNNNRTG 7 cut(s) 1761, 2296, 3624, 4371, 5442, 5448, 5986
SacI GAGCTC 1 cut(s) 1446
SalI GTCGAC 1 cut(s) 1338
Sau96I GGNCC 9 cut(s) 1304, 2251, 2386, 3385, 4640, 5493, 5605, 5824, 6558
ScaI AGTACT 3 cut(s) 2331, 5225, 5710
SfcI CTRYAG 9 cut(s) 84, 672, 1596, 3140, 3659, 4328, 5102, 5146, 5974
Sfr274I CTCGAG 4 cut(s) 1110, 2018, 2885, 6715
SfuI TTCGAA 3 cut(s) 1068, 2564, 3344
SinI GGWCC 7 cut(s) 2251, 2386, 3385, 5493, 5605, 5824, 6558
SlaI CTCGAG 4 cut(s) 1110, 2018, 2885, 6715
SmiMI CAYNNNNRTG 7 cut(s) 1761, 2296, 3624, 4371, 5442, 5448, 5986
SnaBI TACGTA 1 cut(s) 5942
SpeI ACTAGT 2 cut(s) 3676, 5716
SseBI AGGCCT 2 cut(s) 928, 2676
SspI AATATT 3 cut(s) 1719, 5055, 6103
SstI GAGCTC 1 cut(s) 1446
StuI AGGCCT 2 cut(s) 928, 2676
StyI CCWWGG 5 cut(s) 447, 738, 3851, 4487, 6306
TaqII GACCGA 2 cut(s) 5052, 5364
TatI WGTACW 6 cut(s) 3, 26, 2014, 2329, 5223, 5708
TauI GCSGC 7 cut(s) 357, 1101, 1557, 2286, 2700, 4008, 5758
TseFI GTSAC 5 cut(s) 1451, 1643, 2099, 6031, 6293
Tsp45I GTSAC 5 cut(s) 1451, 1643, 2099, 6031, 6293
TspGWI ACGGA 9 cut(s) 1067, 1445, 1751, 2928, 5478, 5982, 6437, 6581, 7124
Tth111I GACNNNGTC 1 cut(s) 692
Van91I CCANNNNNTGG 5 cut(s) 2467, 3466, 4620, 4901, 5644
Vha464I CTTAAG 4 cut(s) 566, 2059, 3395, 3569
VneI GTGCAC 1 cut(s) 3437
VpaK11BI GGWCC 7 cut(s) 2251, 2386, 3385, 5493, 5605, 5824, 6558
VspI ATTAAT 2 cut(s) 6105, 6983
XagI CCTNNNNNAGG 1 cut(s) 446
XbaI TCTAGA 7 cut(s) 1255, 2221, 2500, 2623, 3172, 4313, 7018
XceI RCATGY 4 cut(s) 851, 1737, 5522, 6377
XcmI CCANNNNNNNNNTGG 2 cut(s) 5265, 5863
XhoI CTCGAG 4 cut(s) 1110, 2018, 2885, 6715
XmaJI CCTAGG 1 cut(s) 3851
XmiI GTMKAC 5 cut(s) 1339, 3183, 4662, 5378, 5938
XmnI GAANNNNTTC 6 cut(s) 441, 615, 1086, 1230, 3285, 4347
ZraI GACGTC 1 cut(s) 337
ZrmI AGTACT 3 cut(s) 2331, 5225, 5710
Zsp2I ATGCAT 2 cut(s) 1872, 3905
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.