FvH4_1g16650

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000307
Physical Location & Seq
Reverse (-)
9635650 .. 9644318
8669 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g16650.t1

Sequence Viewer

Length: 5493 bp
ATGGATGCAGCTGCGTCATCATCTTCAACTTCTTCATCATCTAGTCCACCCTCTAATTGGGAGCATGATGTGTTCCTCAGTTTCAGGGGCAAGGACACCCGCCATGGTTTCACAGACCATTTATACTTTGCTCTGAATCAGAGAGGAATTGACACATATAGGGATGCAGAGAAACTTCCAAGGGGAAAGTACATGTCTTTAGAACTTGTCGAAGCAATTCAGGAGTCAAGGTTTGCGGTCATTGTTCTTTCAGCTAACTATGCCACTTCAAGTTGGTGTTTAGGTGAGCTTGCTCATATTCTTGAATGCAAGAAACTGAGGGGACTGGAAGTTCTTCCGGTTTTTTACCATGTAGAGCCATCCGAGATAAGGAAGCAAGCTGGAGGGTTGGGCATGGAGATTGCCAAGCATGAGATGGATTTCGAGGAGAAGAGAAGGAAGGTGGACAAGTGGAAGGAAGCTTTGAAGGAGATAGCCAGTCTCTCCGGATGGCATGTCACCAAGGACAGGGGAGAATCAGAAGTTATTCAGGAAATAGTTAAATGTATTTCAGACATGTTGAACAACATGTTATGCAACCCGGACAGAGATTTAATTGGGATGGATGCACGTATTGAGAAAATGGAGTGGTACTTGGATCTAAGGTCGAGTGATGTTCTTGCGGTAGGGATTTGGGGGATGGGGGGAATTGGTAAGACAACTCTGGCAAAAGAAGTTTTCAAAAAGATTCGTAACCAATTTGATATTAGCGGTTTTGTTTCCGATGTGAGATTGTATTCAGAAGAAAAGGCTCTAGTTGAGTTGCAGAAACTTCTTTGTGGTTCCTTCTTCGGGGACAGCAATATAAATATAGACACTGTCGAAAGAGGGATCAGGTTATTGAAGAAGGTACTATGCAAGAAAAAGGTGCTTCTCGTACTAGATGACGTTGACAAACTTAAACAACTGAAAGATTTAGCACCTGGAATGCATAATGAAGAGAATTCTTGGGCGCCAGGGAGTCGACTCATTATAACAACTAGAGATAGGCGCATATTGACAGAATGTGGAGTACTGGAGTCTAAAATATATGAGGTTGAAAAACTGAGTGATGTAGAAGGATTTCAGCTCATATGTCAACAAGCCTTCAAGAAAAACTATCCACCAGTTGAGTTTGTAGAGCTGTCCAAGAGTTTCATAAAATATGCCAGTGGCCTTCCTTTAGCTCATAAAGTTCTAGGATCACACTTGTATGGTAGGGAGATAGATGAATGGTTAGAGGTGTTGTATAGACTGGACGAAGAGCTGGATAAAGATATTTTTAGTGTGCTCCAAATAAGTTTTGATGGATTACATGAGACTGATAAGAAAATCTTTTTGGACATTGCATGTTTCTTCAATGGTGAGGATCATGTTCGTGTAAAAAATATATTGAAAGGTTGTGGCTTTTCTTCCGGAATAGGCATAACTAACCTTATCAATAAATCTTTGATTAAGATTGAAAGGAAGAAACTGTGGATGCATGATTTACTGCGGTGCTTGGGTTGGCATATTGTCCGTAGAGAATCTGTTGTCCCAGGTAAACGTAGCAGGTTGTGGCTTGATGACAAATCATTTGACTACAAACGCAGAAGGTTATGGCATGTGGAGGATGCTCACAATGTAATCACGGAAAATACAGGAACAACTTCTATTGAAGGCTTGTTCTTAAGCTTGCATGAAAAAGAAGAAATACGTTTGAATGAAGACCCGTTCTTAACTATGCACAACCTAAGATTGTTGAAGATTTGTAATGTGAACTTTCATGGTGTGCAGTTCACCTATCTTTCTAGAAAATTACGGTTTTTGGAATGGCATGAATGCCCCCTAGAATCTTTGTCATCTTGCTTTACACCGGATTCACTAGTAAGCCTGGTTGAATTAAAGATTCCTAACAGCCGCATTGAACAAGTATGGAATGGAAGGATATCTCTGGAAATGCTAATACTCATGGATCTTTCCAACTGCCAATATTTAACGATGACCCCGGACTTCAGTACGGTCCCGAATCTTGAGCAGTTGATCCTCCAAGGTTGTAAGAAGTTATCAGAGGTTCACCATACAATCGGGAATCTTCAGAAACTGGGTATTTTGAACTTGAAAGGCTGCACAAGTCTAGAGAGTCTCCCCCAATATATTGGCTTGTCATCTCTCCAAACTTTTATTCTGTCAGGATGTTCAAAACTCCAAGAGTTTCCGCAGATTTTGGAGAATATGGATACGTTATCAGAACTTTATCTAGATGGCACGGCTATTTGGCAACTACCTGAATCAATCCAGCATTTGAAAGGCCTTGTTTGGCTAAATCTAAGCGGCTGCACGAACCTTCTCAGTATTCCAAGCATCCTTTGTAGTAGTTTGACATCCCTGAAATTTCTCTGTCTGTCACTGTGCTCAAGTATGGACAATCTGCCGGATAACATAGGCTCCTTGGAACACTTGGAGGAGCTCGATGCATGTAATACTGCTATAAGAAAAGTGCCCGAGTCCACATCTCACCTCAAGAATCTTAAACTATTGTGTTTTCATGGCTGCTCTGGATTCACAGGTTTAGAATTGCCAAACAAGTTCTCAGGCTTGAGGTCTTTGACAACACTAAACCTAGGTGGATGTAATCTCGCAGAAGGTGCAATCCCTGATGACATTGGTCATTTATTCTCATTGCAGAGTCTTGATTTAAGTGAGAATGACTTTTTCACCATACCTGAAAGCATCTCCCAGCTTTCTGAGCTTACAGAAATTTCGCTGTTTAAGTGTAGCAATCTACAGTCATTGCCAAAAGATCTTCCATCAAGTCTAAGAAATGTAAATCTGCGTGGTTGTCCTATGCTGACAAATGCTTCATCTAATTGGAGGAGATATCCGCCTCAAAAGGTTTTGAGTATCATAAACTGTCGAAGACCAGAACACGAGGAGGAGGAGGAAATCTTTCCTGAACTCCATAAGTTTCGTTTAGGAAATCTGGAAGTACTCGATCTTTCCTACTGCCAACACTTGAAGAAGGTCCCTGACTTGAATGGGGTTCCAAATCTTAAGAAACTGAACCTTGAAGGTTGTGAAAAGCTATCTGAGGTTCACCCTACAGTTGGGCGTCTCCAACATCTGATTGTTCTGAATATGAAAGGATGTGTTGATCTAGAGAAATTTCCCCGTTCCATCAGCTTGAAGTCTCTAAAATTATTTGATCTTTCAGGATGTTCAAAACTGAAAGAGTTTCCAGAGATTAATGGAGATATGGGGAAATTTTCACAACTGCATTTAGATGGGACGGCGTTAAAGTATCTGCCAATACCAATGAAGCAATTCACATGCCCTATTTCGATAACTCTAAGAGGCTGCAGGAACCTTTTGACTGTTCCGGTCTTGTTAAGTCTGAATTCTCTCAATCTGTCAGGCTGCTCCCGTATAACTAGACTGCCAGCAGACCTGGGAACAATGGTACATTTGGAGGAGCTTGATGCCTCCGAAACTTCCATAACTGAAGTACCCTATTCCATTTTATGCTTGGAGAAGCTCAAAGTGTTATCTTTCTGTGGATGTTTTGGTTTGCAGTTACCTGACTGGTTCTCGAATTTAAGATCTTTGAGATCATTAAATCTACGTAAGTGTGGTCTACCAAAAAATTTTCATGATAGGCTCTTCTGGTTGACTTCACTGCAGAGTCTGGATTTGGGTGAAAATGATTTGGTGAGTATACCTAATGAACTTGGTAGACTATCCTTTTTGCAGAGATTGGATTTCAGTGGAAACAATTTAGTTAGTGTACCAAATGAAATTGGTTGCCTATCCTCTTTGCAGCGTTTAGATTTGAGTGAAAACAACTTTGTGAGTATACCTGAAAGCATCTCTCAACTTTCTCGGCTTACAGAACTTCGCTTGTTTAGGTGTAGTGTGTTACAGTCTTTGCCAAGAAATCTTCCATTCAATCTAAAATATGTCAATGCACGAGAATGTCCCATGTTGAAGAATAATGCCGATACTTTGACAATGTGGGCTTCCGGGAAACGGTTCTGCTTCATAAATTGTGGACAATCAGAAGAGGATAATGTTCAGGCAAGCCATGTTCCAGTTCCAGAAGACAATATTGGACTACTCTTTTCCAAATACATTCAGAATCGAATCTATGGTAAAAAACCATTCGAATTTCGTTTTCCACATAGTACAAGAATCCCCCATTTGTGGAGCCATTGGAGGAGTGGCCCTTCTGTGGCAGTCCCGCTATCTGATAGTAATAGTTCGTGGATTGGATTTGCTCTATTTGTTGTTTTCGAAATCCTTGAGAAAGAAGATTTCGACAGCAGTTGGGAATCAGAGGAGACTATTTGTGAATTTCATACCAATTTTGGGTGTGAAAATTCACTAGTTTTCCAAAATTTCCCTGACTTCAGGACTGGTCCATATGGATTTTGTTGCTACGAACCACGAGTTGGGAAATTTGGTGGCTTGTTCGATAAATTAGTACTTCAAGCTACAGTTTCAACAAAGAGACCAGATTTGAAAGTGAGAGGTTGTGGGTTGCATCTAATATCAGAGCACAATGCTGCAGAGTTTGTTCAAAGTTTAATAAACCAGACTGCTATTCAGCACCTCGATTTCAATTTTGATCGACATTGTGAGGAAATATTAGAAAAGGCAACGACTTGTGACCTGATAGATGTAGACCCTGACGATCTATGGGAACTAGATTCTACATCAACTATGAATACAGATAACTGCCACATGAGTAGCTGCAGTATCCAAATCAGAAAACAGCTCTCATTCCTCTATGAGGGACGCAATGGATGTGAGAAGAGTTTCCATGTTTGTCTTCCTGATTCATCAGTTGTACTGACATCTCCGACCTGGTTCATCAAGCGCCACGCAGGTGATGTAACACTATGTTACATCAGTGAAAACTTACATGCTAATCAGAGATGGGTGGGATTGGAGCTATTTGTTATATTTGGCCGGCGGACACGTGCTTCAAGTGATAACCATAATAACAGTTTCGCCTTTCATATTGAGTTGTGTGGTCATGATAATGAAATTCTGATAATGCATGAATTCCTCAAGAGACATAGTTGTCTTGGAGAGACGAATCAACTTGTGGTATTACATTTACCACGTGTTTATTTTCAACAACAGTTGAATCAATGTCAGGTTATTAGCGCATTGTTTACAACCATTAACCCAGAATTGGAGGTTGTAATGTGTGGGAGCCGTCTAGTTTATCAGCAAGATTTACGAGACTTGATCCGTTCATTGACTGCGGCTGCAAGAAAGTTTGGGGAACATGTCCTTACTCAACTTTGTTCCCTGGCCTGCACTCAAACTGATGATCAGATCATTGCAGTGGAAGGAGAAATGCCCATCAATTGTGATTCCTCTTCTCAAAGAAGTACAGCACAAGTACCGGAACAAGCTTTAACTTCAATAATGAGGCTGAAAAGTTATAGATGCTATCTCCGGCAAAAGCAAGTTTTTTGTAAATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 LRR_8 NB-ARC TIR

Protein Analysis

1831

Amino Acids

207.87

Weight (kDa)

6.11

Isoelectric Point (pI)

47.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 22 - 193 2e-51 TIR domain
TIR_2 PF13676 24 - 120 2.5e-14 TIR domain
NB-ARC PF00931 215 - 379 5.7e-22 NB-ARC domain
NACHT PF05729 224 - 373 1.7e-06 NACHT domain
WHD_ROQ1 PF23282 446 - 516 3.8e-17 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 666 - 775 6.5e-07 Leucine-rich repeat region
LRR_13 PF23286 721 - 789 1.1e-06 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 774 - 874 2.8e-11 Leucine-rich repeat region
LRR_14 PF23598 1120 - 1230 4.6e-12 Leucine-rich repeat region
LRR_14 PF23598 1204 - 1302 3.7e-10 Leucine-rich repeat region
LRR_8 PF13855 1248 - 1302 3.3e-06 Leucine rich repeat
LRR_13 PF23286 1265 - 1345 6.9e-06 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000307)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g16600 FvH4_1g16610 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16621 FvH4_1g16630 FvH4_1g16640 FvH4_1g16640 FvH4_1g16650 FvH4_1g16650 FvH4_3g27631 FvH4_4g11392 FvH4_4g11542
prunus_persica Prupe.4G224500_v2.0.a1 Prupe.4G226900_v2.0.a1 Prupe.4G227000_v2.0.a1
pyrus_communis pycom11g18180 pycom11g18210 pycom11g18230 pycom11g18270 pycom11g18350 pycom11g18360 pycom11g18370
rosa_chinensis RchiOBHm_Chr2g0106511 RchiOBHm_Chr2g0106541 RchiOBHm_Chr2g0106551 RchiOBHm_Chr2g0106571 RchiOBHm_Chr2g0124621 RchiOBHm_Chr6g0251591 RchiOBHm_Chr6g0251631 RchiOBHm_Chr6g0251641
rosa_laevigata RLG00000015443 RLG00000015444 RLG00000015458 RLG00000017510 RLG00000017511 RLG00000017512 RLG00000017515 RLG00000017516 RLG00000017517 RLG00000018809 RLG00000020934 RLG00000020935 RLG00000034092
rosa_multiflora Rmu_co8285777.1_g000001 Rmu_sc0000762.1_g000001 Rmu_sc0001872.1_g000006 Rmu_sc0005255.1_g000019 Rmu_sc0005597.1_g000006 Rmu_sc0012104.1_g000001 Rmu_sc0017105.1_g000011 Rmu_sc0022904.1_g000001 Rmu_ssc0000135.1_g000049 Rmu_ssc0000213.1_g000047
rosa_roxburghii Rroxscaffold_2G00092830 Rroxscaffold_2G00119790 Rroxscaffold_2G00137010 Rroxscaffold_2G00137030 Rroxscaffold_2G00137070 Rroxscaffold_7G00216560
rosa_rugosa Rorug02G0136100 Rorug02G0136200 Rorug02G0136300 Rorug02G0136400 Rorug02G0251800 Rorug02G0251800 Rorug02G0455500 Rorug02G0455600 Rorug02G0455700 Rorug05G0195300
rosa_samantha Rh2AG187400 Rh2AG187500 Rh2AG187600 Rh2AG310500 Rh2BG198200 Rh2BG198300 Rh2BG198400 Rh2BG198600 Rh2BG318900 Rh2BG534300 Rh2CG192400 Rh2CG192500 Rh2CG192600 Rh2CG297500 Rh2DG193500 Rh2DG193600 Rh2DG193800 Rh2DG334200 Rh2DG543100 Rh5AG280400 Rh5BG285800 Rh5BG285900 Rh5CG317900 Rh5CG318000 Rh5DG294600 Rh5DG294700 Rh6AG012200 Rh6AG012700 Rh6BG011200 Rh6BG011700
rosa_wichuraiana Rw0G009380 Rw2G014610 Rw2G014630 Rw2G014640 Rw2G014680 Rw2G025120 Rw2G043000 Rw5G026490 Rw6G001370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1013
AarI CACCTGC 1 cut(s) 4871
AasI GACNNNNNNGTC 1 cut(s) 5079
Acc36I ACCTGC 2 cut(s) 1560, 4871
AccB1I GGYRCC 1 cut(s) 991
AccB7I CCANNNNNTGG 1 cut(s) 4445
AccI GTMKAC 6 cut(s) 1003, 3631, 3712, 3730, 3850, 4677
AccIII TCCGGA 2 cut(s) 485, 1433
AclWI GGATC 7 cut(s) 645, 878, 1228, 1395, 1980, 2035, 5245
AcoI YGGCCR 1 cut(s) 4963
AcuI CTGAAG 4 cut(s) 1996, 2078, 3519, 4387
AcvI CACGTG 2 cut(s) 4976, 5123
AcyI GRCGYC 2 cut(s) 992, 3109
AflII CTTAAG 2 cut(s) 1687, 3050
AflIII ACRYGT 6 cut(s) 192, 555, 567, 4973, 5122, 5290
AhlI ACTAGT 2 cut(s) 1882, 4378
AjnI CCWGG 7 cut(s) 961, 994, 1555, 1890, 3444, 4859, 5313
AjuI GAANNNNNNNTTGG 8 cut(s) 1340, 1372, 2166, 2198, 4086, 4118, 4428, 4460
AleI CACNNNNGTG 1 cut(s) 4881
AloI GAACNNNNNNTCC 2 cut(s) 315, 347
Alw21I GWGCWC 4 cut(s) 1311, 2414, 2469, 4554
AlwI GGATC 7 cut(s) 645, 878, 1228, 1395, 1980, 2035, 5245
AlwNI CAGNNNCTG 2 cut(s) 2101, 3682
Ama87I CYCGRG 1 cut(s) 2501
Aor13HI TCCGGA 2 cut(s) 485, 1433
AoxI GGCC 5 cut(s) 1192, 2308, 4216, 4963, 5316
ArsI GACNNNNNNTTYG 2 cut(s) 1577, 1609
AseI ATTAAT 1 cut(s) 3243
Asp700I GAANNNNTTC 9 cut(s) 216, 333, 525, 1101, 1666, 2946, 3320, 4025, 4811
AspA2I CCTAGG 1 cut(s) 2620
AspLEI GCGC 4 cut(s) 994, 1032, 4875, 5168
AspS9I GGNCC 4 cut(s) 2020, 3022, 4217, 4412
AsuC2I CCSGG 3 cut(s) 581, 2006, 4018
AsuII TTCGAA 2 cut(s) 4158, 4287
AvaI CYCGRG 1 cut(s) 2501
AvaII GGWCC 3 cut(s) 2020, 3022, 4412
AvrII CCTAGG 1 cut(s) 2620
BaeGI GKGCMC 1 cut(s) 2502
BaeI ACNNNNGTAYC 2 cut(s) 3486, 3519
BanI GGYRCC 1 cut(s) 991
BanII GRGCYC 1 cut(s) 2469
BauI CACGAG 3 cut(s) 2927, 3963, 4440
BbrPI CACGTG 2 cut(s) 4976, 5123
BbsI GAAGAC 4 cut(s) 1731, 2923, 4101, 4817
Bbv12I GWGCWC 4 cut(s) 1311, 2414, 2469, 4554
BceAI ACGGC 3 cut(s) 2283, 3303, 5202
BciT130I CCWGG 7 cut(s) 963, 996, 1557, 1892, 3446, 4861, 5315
BciVI GTATCC 2 cut(s) 2230, 4763
BclI TGATCA 1 cut(s) 5335
BcnI CCSGG 3 cut(s) 581, 2006, 4018
BcuI ACTAGT 2 cut(s) 1882, 4378
BfmI CTRYAG 7 cut(s) 2783, 3099, 3355, 3674, 4488, 4560, 4747
BfoI RGCGCY 2 cut(s) 995, 4876
BfrI CTTAAG 2 cut(s) 1687, 3050
BfuAI ACCTGC 2 cut(s) 1560, 4871
BfuI GTATCC 2 cut(s) 2230, 4763
BglII AGATCT 2 cut(s) 2800, 3596
BlnI CCTAGG 1 cut(s) 2620
BmcAI AGTACT 3 cut(s) 1053, 2988, 4479
Bme18I GGWCC 3 cut(s) 2020, 3022, 4412
BmeT110I CYCGRG 1 cut(s) 2501
BmgT120I GGNCC 4 cut(s) 2020, 3022, 4217, 4412
BmiI GGNNCC 9 cut(s) 823, 993, 2022, 2446, 3024, 3042, 3362, 4202, 5216
BmrI ACTGGG 1 cut(s) 2111
BmuI ACTGGG 1 cut(s) 2111
BoxI GACNNNNGTC 1 cut(s) 2664
BpiI GAAGAC 4 cut(s) 1731, 2923, 4101, 4817
BpmI CTGGAG 2 cut(s) 402, 1076
Bpu14I TTCGAA 2 cut(s) 4158, 4287
BpuEI CTTGAG 6 cut(s) 2051, 2398, 2504, 2617, 4316, 5051
BpuMI CCSGG 3 cut(s) 581, 2006, 4018
BsaAI YACGTR 4 cut(s) 611, 3620, 4976, 5123
BsaHI GRCGYC 2 cut(s) 992, 3109
BsaI GGTCTC 1 cut(s) 4498
BsaWI WCCGGW 6 cut(s) 337, 485, 1433, 1873, 3376, 5410
BsaXI ACNNNNNCTCC 4 cut(s) 215, 245, 2429, 2459
Bse118I RCCGGY 1 cut(s) 4965
Bse3DI GCAATG 5 cut(s) 1362, 2678, 2789, 4801, 5343
BseAI TCCGGA 2 cut(s) 485, 1433
BseBI CCWGG 7 cut(s) 963, 996, 1557, 1892, 3446, 4861, 5315
BseMI GCAATG 5 cut(s) 1362, 2678, 2789, 4801, 5343
BseMII CTCAG 7 cut(s) 91, 308, 1076, 2362, 2604, 2736, 3078
BseRI GAGGAG 9 cut(s) 440, 2477, 2887, 2945, 2948, 2951, 3482, 4225, 4346
BseSI GKGCMC 1 cut(s) 2502
BseYI CCCAGC 1 cut(s) 2736
BsgI GTGCAG 4 cut(s) 1811, 2110, 2320, 5305
BshFI GGCC 5 cut(s) 1194, 2310, 4218, 4965, 5318
BshNI GGYRCC 1 cut(s) 991
BsiHKAI GWGCWC 4 cut(s) 1311, 2414, 2469, 4554
BsiHKCI CYCGRG 1 cut(s) 2501
BslFI GGGAC 9 cut(s) 336, 848, 1538, 2006, 3008, 3298, 3957, 4217, 4803
BsmBI CGTCTC 2 cut(s) 3116, 5084
BsmFI GGGAC 9 cut(s) 336, 848, 1538, 2006, 3008, 3298, 3957, 4217, 4803
BsmI GAATGC 3 cut(s) 311, 972, 1844
BsnI GGCC 5 cut(s) 1194, 2310, 4218, 4965, 5318
Bso31I GGTCTC 1 cut(s) 4498
BsoBI CYCGRG 1 cut(s) 2501
Bsp119I TTCGAA 2 cut(s) 4158, 4287
Bsp1286I GDGCHC 5 cut(s) 1311, 2414, 2469, 2502, 4554
Bsp13I TCCGGA 2 cut(s) 485, 1433
Bsp19I CCATGG 1 cut(s) 103
BspANI GGCC 5 cut(s) 1194, 2310, 4218, 4965, 5318
BspCNI CTCAG 7 cut(s) 90, 309, 1077, 2361, 2603, 2737, 3079
BspEI TCCGGA 2 cut(s) 485, 1433
BspHI TCATGA 2 cut(s) 3646, 5032
BspLI GGNNCC 9 cut(s) 823, 993, 2022, 2446, 3024, 3042, 3362, 4202, 5216
BspMAI CTGCAG 4 cut(s) 3359, 3678, 4564, 4751
BspMI ACCTGC 2 cut(s) 1560, 4871
BspPI GGATC 7 cut(s) 645, 878, 1228, 1395, 1980, 2035, 5245
BspQI GCTCTTC 2 cut(s) 1275, 3662
BspT104I TTCGAA 2 cut(s) 4158, 4287
BspT107I GGYRCC 1 cut(s) 991
BspTI CTTAAG 2 cut(s) 1687, 3050
BspTNI GGTCTC 1 cut(s) 4498
BsrDI GCAATG 5 cut(s) 1362, 2678, 2789, 4801, 5343
BsrFI RCCGGY 1 cut(s) 4965
BssAI RCCGGY 1 cut(s) 4965
BssNAI GTATAC 2 cut(s) 3713, 3851
BssNI GRCGYC 2 cut(s) 992, 3109
BssSI CACGAG 3 cut(s) 2927, 3963, 4440
BssT1I CCWWGG 6 cut(s) 103, 179, 501, 2047, 2448, 2620
Bst1107I GTATAC 2 cut(s) 3713, 3851
Bst2BI CACGAG 3 cut(s) 2927, 3963, 4440
Bst2UI CCWGG 7 cut(s) 963, 996, 1557, 1892, 3446, 4861, 5315
Bst6I CTCTTC 7 cut(s) 425, 972, 1275, 3662, 4050, 4802, 5389
BstACI GRCGYC 2 cut(s) 992, 3109
BstAFI CTTAAG 2 cut(s) 1687, 3050
BstAPI GCANNNNNTGC 1 cut(s) 2645
BstBAI YACGTR 4 cut(s) 611, 3620, 4976, 5123
BstBI TTCGAA 2 cut(s) 4158, 4287
BstC8I GCNNGC 7 cut(s) 291, 378, 1694, 3438, 4075, 4967, 5320
BstDSI CCRYGG 1 cut(s) 103
BstENI CCTNNNNNAGG 1 cut(s) 4784
BstH2I RGCGCY 2 cut(s) 995, 4876
BstHHI GCGC 4 cut(s) 994, 1032, 4875, 5168
BstMWI GCNNNNNNNGC 3 cut(s) 260, 2645, 2746
BstNI CCWGG 7 cut(s) 963, 996, 1557, 1892, 3446, 4861, 5315
BstPAI GACNNNNGTC 1 cut(s) 2664
BstSFI CTRYAG 7 cut(s) 2783, 3099, 3355, 3674, 4488, 4560, 4747
BstSLI GKGCMC 1 cut(s) 2502
BstSNI TACGTA 1 cut(s) 3620
BstV2I GAAGAC 4 cut(s) 1731, 2923, 4101, 4817
BstX2I RGATCY 4 cut(s) 637, 1972, 2800, 3596
BstXI CCANNNNNNTGG 1 cut(s) 2156
BstYI RGATCY 4 cut(s) 637, 1972, 2800, 3596
BstZ17I GTATAC 2 cut(s) 3713, 3851
BsuI GTATCC 2 cut(s) 2230, 4763
BsuRI GGCC 5 cut(s) 1194, 2310, 4218, 4965, 5318
BtgI CCRYGG 1 cut(s) 103
BtsI GCAGTG 2 cut(s) 3671, 5355
BtsIMutI CAGTG 7 cut(s) 855, 1195, 2405, 3671, 3766, 4912, 5355
BveI ACCTGC 2 cut(s) 1560, 4871
Cac8I GCNNGC 7 cut(s) 291, 378, 1694, 3438, 4075, 4967, 5320
CaiI CAGNNNCTG 2 cut(s) 2101, 3682
CciI TCATGA 2 cut(s) 3646, 5032
CfoI GCGC 4 cut(s) 994, 1032, 4875, 5168
Cfr10I RCCGGY 1 cut(s) 4965
Cfr13I GGNCC 4 cut(s) 2020, 3022, 4217, 4412
CseI GACGC 3 cut(s) 3, 3098, 4800
CsiI ACCWGGT 1 cut(s) 4859
DinI GGCGCC 1 cut(s) 993
DrdI GACNNNNNNGTC 1 cut(s) 5079
DseDI GACNNNNNNGTC 1 cut(s) 5079
EaeI YGGCCR 1 cut(s) 4963
Eam1104I CTCTTC 7 cut(s) 425, 972, 1275, 3662, 4050, 4802, 5389
EarI CTCTTC 7 cut(s) 425, 972, 1275, 3662, 4050, 4802, 5389
EciI GGCGGA 2 cut(s) 2871, 4984
Ecl136II GAGCTC 1 cut(s) 2467
Eco105I TACGTA 1 cut(s) 3620
Eco130I CCWWGG 6 cut(s) 103, 179, 501, 2047, 2448, 2620
Eco147I AGGCCT 1 cut(s) 2310
Eco24I GRGCYC 1 cut(s) 2469
Eco31I GGTCTC 1 cut(s) 4498
Eco32I GATATC 2 cut(s) 1947, 2879
Eco47I GGWCC 3 cut(s) 2020, 3022, 4412
Eco53kI GAGCTC 1 cut(s) 2467
Eco57I CTGAAG 4 cut(s) 1996, 2078, 3519, 4387
Eco72I CACGTG 2 cut(s) 4976, 5123
Eco88I CYCGRG 1 cut(s) 2501
EcoICRI GAGCTC 1 cut(s) 2467
EcoNI CCTNNNNNAGG 1 cut(s) 4784
EcoO109I RGGNCCY 1 cut(s) 3022
EcoRI GAATTC 3 cut(s) 982, 3394, 5060
EcoRII CCWGG 7 cut(s) 961, 994, 1555, 1890, 3444, 4859, 5313
EcoRV GATATC 2 cut(s) 1947, 2879
EcoT14I CCWWGG 6 cut(s) 103, 179, 501, 2047, 2448, 2620
EcoT22I ATGCAT 4 cut(s) 972, 1503, 2476, 5058
EcoT38I GRGCYC 1 cut(s) 2469
EgeI GGCGCC 1 cut(s) 993
EheI GGCGCC 1 cut(s) 993
ErhI CCWWGG 6 cut(s) 103, 179, 501, 2047, 2448, 2620
Esp3I CGTCTC 2 cut(s) 3116, 5084
FalI AAGNNNNNCTT 2 cut(s) 1337, 1369
FaqI GGGAC 9 cut(s) 336, 848, 1538, 2006, 3008, 3298, 3957, 4217, 4803
FauI CCCGC 2 cut(s) 107, 4242
FauNDI CATATG 2 cut(s) 1112, 4417
FbaI TGATCA 1 cut(s) 5335
FblI GTMKAC 6 cut(s) 1003, 3631, 3712, 3730, 3850, 4677
FriOI GRGCYC 1 cut(s) 2469
GlaI GCGC 4 cut(s) 993, 1031, 4874, 5167
GsaI CCCAGC 1 cut(s) 2740
GsuI CTGGAG 2 cut(s) 402, 1076
HaeII RGCGCY 2 cut(s) 995, 4876
HaeIII GGCC 5 cut(s) 1194, 2310, 4218, 4965, 5318
HgaI GACGC 3 cut(s) 3, 3098, 4800
HhaI GCGC 4 cut(s) 994, 1032, 4875, 5168
Hin1I GRCGYC 2 cut(s) 992, 3109
Hin6I GCGC 4 cut(s) 992, 1030, 4873, 5166
HinP1I GCGC 4 cut(s) 992, 1030, 4873, 5166
HincII GTYRAC 4 cut(s) 931, 1004, 1118, 3666
HindII GTYRAC 4 cut(s) 931, 1004, 1118, 3666
HindIII AAGCTT 3 cut(s) 459, 1690, 5418
HpyCH4IV ACGT 8 cut(s) 610, 927, 1564, 1714, 2240, 3619, 4975, 5122
HpyF10VI GCNNNNNNNGC 3 cut(s) 260, 2645, 2746
HpySE526I ACGT 8 cut(s) 610, 927, 1564, 1714, 2240, 3619, 4975, 5122
Hsp92I GRCGYC 2 cut(s) 992, 3109
HspAI GCGC 4 cut(s) 992, 1030, 4873, 5166
KasI GGCGCC 1 cut(s) 991
Kpn2I TCCGGA 2 cut(s) 485, 1433
KroI GCCGGC 1 cut(s) 4965
KroNI GCCGGC 1 cut(s) 4967
Ksp22I TGATCA 1 cut(s) 5335
LguI GCTCTTC 2 cut(s) 1275, 3662
LmnI GCTCC 9 cut(s) 61, 1314, 2450, 2464, 3422, 3469, 4200, 4945, 5214
MabI ACCWGGT 1 cut(s) 4859
MaeII ACGT 8 cut(s) 610, 927, 1564, 1714, 2240, 3619, 4975, 5122
MaeIII GTNAC 8 cut(s) 496, 731, 2403, 3570, 3912, 4661, 4888, 4898
MfeI CAATTG 1 cut(s) 5371
MflI RGATCY 4 cut(s) 637, 1972, 2800, 3596
MhlI GDGCHC 5 cut(s) 1311, 2414, 2469, 2502, 4554
Mly113I GGCGCC 1 cut(s) 992
MlyI GAGTC 8 cut(s) 233, 999, 1009, 1067, 2149, 2513, 2695, 3688
MmeI TCCRAC 3 cut(s) 2004, 3139, 4880
Mph1103I ATGCAT 4 cut(s) 972, 1503, 2476, 5058
MroI TCCGGA 2 cut(s) 485, 1433
MroNI GCCGGC 1 cut(s) 4965
MroXI GAANNNNTTC 9 cut(s) 216, 333, 525, 1101, 1666, 2946, 3320, 4025, 4811
MslI CAYNNNNRTG 7 cut(s) 1230, 1395, 3279, 3967, 4881, 5037, 5348
MspA1I CMGCKG 1 cut(s) 11
MspCI CTTAAG 2 cut(s) 1687, 3050
MunI CAATTG 1 cut(s) 5371
Mva1269I GAATGC 3 cut(s) 311, 972, 1844
MvaI CCWGG 7 cut(s) 963, 996, 1557, 1892, 3446, 4861, 5315
MwoI GCNNNNNNNGC 3 cut(s) 260, 2645, 2746
NaeI GCCGGC 1 cut(s) 4967
NarI GGCGCC 1 cut(s) 992
NciI CCSGG 3 cut(s) 581, 2006, 4018
NcoI CCATGG 1 cut(s) 103
NdeI CATATG 2 cut(s) 1112, 4417
NgoMIV GCCGGC 1 cut(s) 4965
NlaIV GGNNCC 9 cut(s) 823, 993, 2022, 2446, 3024, 3042, 3362, 4202, 5216
NmeAIII GCCGAG 1 cut(s) 3856
NmuCI GTSAC 3 cut(s) 496, 2403, 4661
NsiI ATGCAT 4 cut(s) 972, 1503, 2476, 5058
NspV TTCGAA 2 cut(s) 4158, 4287
OliI CACNNNNGTG 1 cut(s) 4881
PagI TCATGA 2 cut(s) 3646, 5032
PaqCI CACCTGC 1 cut(s) 4871
PceI AGGCCT 1 cut(s) 2310
PciI ACATGT 4 cut(s) 192, 555, 567, 5290
PciSI GCTCTTC 2 cut(s) 1275, 3662
PctI GAATGC 3 cut(s) 311, 972, 1844
PdiI GCCGGC 1 cut(s) 4967
PdmI GAANNNNTTC 9 cut(s) 216, 333, 525, 1101, 1666, 2946, 3320, 4025, 4811
PflFI GACNNNGTC 1 cut(s) 857
PflMI CCANNNNNTGG 1 cut(s) 4445
PfoI TCCNGGA 1 cut(s) 4016
PleI GAGTC 8 cut(s) 232, 999, 1008, 1066, 2148, 2512, 2694, 3687
PluTI GGCGCC 1 cut(s) 995
PmaCI CACGTG 2 cut(s) 4976, 5123
PmlI CACGTG 2 cut(s) 4976, 5123
PpsI GAGTC 8 cut(s) 232, 999, 1008, 1066, 2148, 2512, 2694, 3687
Ppu21I YACGTR 4 cut(s) 611, 3620, 4976, 5123
PpuMI RGGWCCY 1 cut(s) 3022
PscI ACATGT 4 cut(s) 192, 555, 567, 5290
PshAI GACNNNNGTC 1 cut(s) 2664
PshBI ATTAAT 1 cut(s) 3243
PsiI TTATAA 1 cut(s) 1013
Psp124BI GAGCTC 1 cut(s) 2469
Psp5II RGGWCCY 1 cut(s) 3022
Psp6I CCWGG 7 cut(s) 961, 994, 1555, 1890, 3444, 4859, 5313
PspCI CACGTG 2 cut(s) 4976, 5123
PspFI CCCAGC 1 cut(s) 2736
PspGI CCWGG 7 cut(s) 961, 994, 1555, 1890, 3444, 4859, 5313
PspN4I GGNNCC 9 cut(s) 823, 993, 2022, 2446, 3024, 3042, 3362, 4202, 5216
PspPI GGNCC 4 cut(s) 2020, 3022, 4217, 4412
PspPPI RGGWCCY 1 cut(s) 3022
PstI CTGCAG 4 cut(s) 3359, 3678, 4564, 4751
PstNI CAGNNNCTG 2 cut(s) 2101, 3682
PsuI RGATCY 4 cut(s) 637, 1972, 2800, 3596
PsyI GACNNNGTC 1 cut(s) 857
PvuII CAGCTG 1 cut(s) 11
RseI CAYNNNNRTG 7 cut(s) 1230, 1395, 3279, 3967, 4881, 5037, 5348
SacI GAGCTC 1 cut(s) 2469
SalI GTCGAC 1 cut(s) 1002
SapI GCTCTTC 2 cut(s) 1275, 3662
Sau96I GGNCC 4 cut(s) 2020, 3022, 4217, 4412
ScaI AGTACT 3 cut(s) 1053, 2988, 4479
SchI GAGTC 8 cut(s) 233, 999, 1009, 1067, 2149, 2513, 2695, 3688
SduI GDGCHC 5 cut(s) 1311, 2414, 2469, 2502, 4554
SexAI ACCWGGT 1 cut(s) 4859
SfcI CTRYAG 7 cut(s) 2783, 3099, 3355, 3674, 4488, 4560, 4747
SfoI GGCGCC 1 cut(s) 993
SfuI TTCGAA 2 cut(s) 4158, 4287
SinI GGWCC 3 cut(s) 2020, 3022, 4412
SmiMI CAYNNNNRTG 7 cut(s) 1230, 1395, 3279, 3967, 4881, 5037, 5348
SmlI CTYRAG 8 cut(s) 1687, 2030, 2413, 2519, 2596, 3050, 4295, 5066
SmoI CTYRAG 8 cut(s) 1687, 2030, 2413, 2519, 2596, 3050, 4295, 5066
SnaBI TACGTA 1 cut(s) 3620
SpeI ACTAGT 2 cut(s) 1882, 4378
SseBI AGGCCT 1 cut(s) 2310
SspDI GGCGCC 1 cut(s) 991
SspI AATATT 3 cut(s) 1991, 4102, 4641
SstI GAGCTC 1 cut(s) 2469
StuI AGGCCT 1 cut(s) 2310
StyI CCWWGG 6 cut(s) 103, 179, 501, 2047, 2448, 2620
TaiI ACGT 8 cut(s) 613, 930, 1567, 1717, 2243, 3622, 4978, 5125
TatI WGTACW 7 cut(s) 189, 1051, 2986, 4178, 4477, 4843, 5396
TauI GCSGC 3 cut(s) 1920, 2334, 5270
TscAI CASTG 7 cut(s) 862, 1195, 2412, 3678, 3766, 4912, 5355
TseFI GTSAC 3 cut(s) 496, 2403, 4661
Tsp45I GTSAC 3 cut(s) 496, 2403, 4661
TspGWI ACGGA 3 cut(s) 1526, 1664, 5243
TspRI CASTG 7 cut(s) 862, 1195, 2412, 3678, 3766, 4912, 5355
Tth111I GACNNNGTC 1 cut(s) 857
Van91I CCANNNNNTGG 1 cut(s) 4445
Vha464I CTTAAG 2 cut(s) 1687, 3050
VpaK11BI GGWCC 3 cut(s) 2020, 3022, 4412
VspI ATTAAT 1 cut(s) 3243
XagI CCTNNNNNAGG 1 cut(s) 4784
XbaI TCTAGA 4 cut(s) 1808, 2134, 2257, 3154
XcmI CCANNNNNNNNNTGG 4 cut(s) 54, 412, 3520, 4211
XmaJI CCTAGG 1 cut(s) 2620
XmiI GTMKAC 6 cut(s) 1003, 3631, 3712, 3730, 3850, 4677
XmnI GAANNNNTTC 9 cut(s) 216, 333, 525, 1101, 1666, 2946, 3320, 4025, 4811
ZrmI AGTACT 3 cut(s) 1053, 2988, 4479
Zsp2I ATGCAT 4 cut(s) 972, 1503, 2476, 5058
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.