Rw2G025120

positive regulation of cell-substrate adhesion

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000307
Physical Location & Seq
Forward (+)
37277772 .. 37286008
8237 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G025120.1

Sequence Viewer

Length: 4752 bp
ATGGACAATACGGGGACAACTACCGTTGAAGGCTTATTCTTAAGCTTGCCTGAAAAAGAAGAAATGCCATTGGAAGATGACCCGTTCTTAACTATGGGCAACCTACGATTGCTGAAGATTTATAATCTAAATTTTCTGGATGTGCACTTCAGATATGTCTCTAAGAATTTACGACTTTTGGAATGGCACGAATGCCCTCTAGTATCTCTGCCATCTAGTTTTAAATCGGACAAGCTGGTTGAATTAAAGATGCCTAACAGCCGCATTGAACAACTATGGAATGAAACGCTTTCTCTGAAAATGCTTATACTCATGGATCTTTCTGACTGCAAGTATTTAACCAAGACCCTGGACTTCAGTAAGGTCCCAAAGCTTGAGAAATTGATCCTTAAAGGTTGTATAGAGTTATTGGAGGTTCACCCTACTATTGGGGATCTCCAACATTTGGTTTTATTGAACTTGAAAGGTTGTGAAAGTCTAGAGAACCTTCCTCAATCCATCAGGTTGAGATCTCTTCGAACTTTTATTCTTTCGGGATGTTCAAAACTCAGACACTTTCCAGAGATTGTGGGGAACATGGATACTTTATCAGAACTTTATTTAGATGGCACGGCTATACGGGAGCTGCCTGTATCAATCCAGCATTTGGAAGGCCTCATTTTACTTAATCTAAGTGGGTGCAGGAACCTTCTCAGTCTTCCAAGCATCCTTTCTAGTAGTTTGACATCACTGAAATTTCTCTATCTGTCACTATGCTCACGTATGGACAGACTGCCGGATAACATAGGCTGCTTGGAACACTTGGAGGAGCTTGATGCGTGTAATACTGCTATAAGAAAAGTGCCCGATTCCATTTTTCTCCTCAAGAATCTTAAATTATTGTGTTTCCATGGCTGTGCTGGATCCACAGGTTTAGAGTTGCCAAGCAAGTTCTCAGGTTTAAGATCTTTGACAACACTAAACCTAGGTGGATGTAATCTCGCAGAAGGAGAAATCCCCAATGACATTGGTGATTTATTCTCACTGCAGAGTCTGGATTTAAGTGAGAACAACTTTTTCACCATACCTGAAAGCATCTCTCAGCTTTCTGAGCTTACAGAAATTTCTCTGTTTAAGTGTAGCAAACTATGGACGTTGCCAAAAGATCTTCCGTCAAGTCTAAGAAATTTAAATGTACGTGGTTGTCCTATGCTGACAAGTTCTTCATCTAGTTGGAGGAGATATCCGCCTCAAAAGGGTTTGAGTATCATAAACTGTCGAAAACCAGAGGGGGATGAAATCTTTCCTGAACTCTATAAGTTTCGTTTGGGAAATCTGGAAGAACTCGATCTTTCCAACTGCCAACACTTGAAGAAGATCCCTGACTTGAATGGGGTTCCAAATCTTAAGAAATTGAACCTTGAAGGTTGTGAAAAATTATCAGAGGTTCACCCAACCATTGGGTGTCTCCAACATTTGGTGTTTCTGAATTTGAAAGGATGTGTAAATCTAGAGAGCCTTCCCAGTTCCATCAGCCTGAAGTCTCTAAACATGTTTGTTCTTTCGGGATGTTCAAAGCTGAAAGAGTTTCCAAACATTAAGGGAGAAATGAACAATTTGTCACAACTGCATTTAGATGGGACGGCGTTAAGGCATCTGCGAATTCCGATGATGCATTTGAAAGGCCCAATTTTGATAAATCTTTCAGGCTGCAGGAACCTTTTGACTGTTCCAATCCTATTCAGTCTAAAATCTCTCAATCTATCACGGTGCTCAACTATATTCAAATTGCCACCGAGCCTGGCAAACATGGAACATTTGGAGGAGCTTGATGCCTCTGAAACTGCTATAACAGGATTACCCCAGTCCATTTTACTCTTGAAGAAACTTAAAGTGTTATCTCTCTGTGGATGCAAAGGTTTGCAATTGCCTAAATGGTTCTCGGATTTAAGCTCTTTGACATCATTAAATCTACGGAGGTGTAGTCTAGCAGAAGAAGTCCTTGATAGCCTCTACAGCTTATCAGCTCTGCAGATTTTGGATTTGAGTGAAAACAATCTTTCGATCATACCTAGTGGAATTGGTCGCTTATCCTCTTTGAAGCTATTGAATTTGAGTGAGAACAATTTTGACAATATACCAAATGAAATCGGTCACTTATCCTCTTTGCAGGTCTTAGATTTGAGTGAAAACAGCTTCATGAGTATACCTGATGAGAGCATCTCTCATCTTTCTGAGCTTACAGAACTACGCTTGTTTAGATGTAGTAAGCTACAGTCCATGCCGAATAATCTTCCCTTCAATCTAAAACATGTCCATGCACAAGAATGTCCTATGTTGAAGAATAACGCAGATACTTTGACAATATATGCATCTGGAAAGGGGTTCTGTTTCATAAATTGCAGCCAATCTGACCGGGATGATGGTCAGCCAAATCACCTCCCAGTTCCAGTTCCCAAAGACCGTATTGAACTACTCTTTCCTATATACATTGAGGACCGAGTTTATGGCAAAAAACCATTTGAAATTCGTTTTCCACATTCGTGGAGTACAAGACTGAGTGCCGGAATTCCAAATTCGTGGAGTCGTTGGAGAAATGGCCCTTCCGTGACAATCCCAGTCTCTGATGGTAACAGTACGTGGATGGGACTTGCTCTTTTTGTTGTTTTTGATATCCTTGAGCAAGACATTTTCAACAAGAGCTGGGAATTGGAGGAGACAGTCTGTGATTTTCACACCAATGTTGGGCATGATACTTCCCTAGTTTTTCAAAACTTCATAGACTTCAGGACTGGGTCATATGGTCTTAGTTGCTATGAACCACGTGGTGGGAAATTTAGTGGGTTGTTCGATAAACCAAGTTCACGACTTCGAGCTTCAGTTTCAACAAAGAGACCAAATTTAAAAGTTAGAGGTTGTGGGATACATCTAATATCAGAGGAATATGCTGCAGAGTTTGTTAAAAGTATAGCAATTCAGACAACTAATTCAACTCAGAGTCATCTTGATTCTAATTTTGATCGACATTGCGAGGACATATTAGATGAGGAAACAACTGGTGTCCTGATAGAACAGGGATCTACTAATTCAACTTTCAATGAAGATAGCTGCAGCAAAGTTAACTCTAAGATCAAACTCAGAGAAAAGCTGTCGATACTCTATGAGGGAAGCAATGGACGTGAGAAGAGTTTCCACTTTTGCTTTCCTGCTTTAGTAATTTCAACCCTGCCTTGGTTCTTCCATCACCATGCAGGGGATGTAACATTGTGTTACATCACTAAAAACTTACTTGATGATCAGAGGTGGGTGGGATTAGAACTATATGTTCAATTTTCTCGGTGTACATCTACTTCGACATCTGGCAATAGTAGTTTTTTCTTTTATGTTGATTTATGCACTCATGATCATGGAAGTATGGTAATGCACGGATCCCTCAAGATAAGGAGTTGTGTTGGGACCTCAGATCAACTTGTTGTATTACATGTACCACGAGTTCATTTTCAACGACAGTTGAATCAATGTCAGGGTATCAGCGCATTGTTCAGAACCATTAATGCAGAAATGGAGGTCCAAGTGTGTGGAAGCCGTTTAGCATTCGAGCATGATTTGGAAGACTTGACCCATTCATTAACAGCAGCTGCCAGCACGTTGGGGCAACATGTCCTCACTCAACTATGTTCCCAGGCCCAACCTGTTGATCGGTGCAATGCAGAGGAAGCAGAAATGCCCATCAATTGCTGTTCCTGTACTGCACTTGTACTAGCAGAACAAGCTCCTTCTTCTTCAATTGTGCGGCTGCGAAATCATAGATGCTATCTCCAGCAACAGCAAGGTTTTGGGGAGTCTGACCTTACCCCATTGGTCCTCTCCAGATCAGTAACATTACTCCACTCGAAAAGCCTTTTGGAAAACAGGGATAGGAACCATGAAAACGTTGCCAAAGAAGATAAATCGTTGGTGTTGGCTCGTCATCATGTACATATAATGGCTGAGACTCAACTTCAGGGAAGATATAGTTCACCACGGTGGAAGAGATGCCTGAAGTTGTTGCTTCGACAGTCCAAGGTGGCTACTCTCAGTCTTTGTGGACGTGCAATTTCAGCTTTCAAGAATTTTGATCCCTTCTCCCCATACAATATTATTTGTTTCTCTGACAAGGAAATTCCAGTGTGGTTCAAACATGAGATGTCGTATCAGATGTCTTCTAGGTCTAGGGTGGGAATCAAACTACCTCCAACATTGCACGAGGATGAGAACTGGAGAGGACTTGCTATATGTGTTGCCTTTGAAGTTCATGACCAGCGTCCAACTACCTCCCCGGTCAAACTTCTCTGTCACTTGAGAGCCAAGGACAACTATTGCTTGAATCCTATCCCTATGTGTTGCATCAATGAAGAGGAACACAAGTCACTGCATCTTGGTAGATTCATTTGGCTAACCTACATTCCCCGTATTTTGCTACCGGAGTTCAGTGTGATTAGTGATGTAGAGGCCAGAATTTATGTTAGTTGCGGAGGCTTGACAGTAGAGAAGAGTGGTATACGTCTCTTGTACAGGCAAGAAGAGGAGAAGTTTGAGAACACAATAACCGAGTGTTGGACATCTTTCTTTGATAATCTGTCTTTCATCCGTCAACTTGTAGAAGCAGATGATCAAAATATTCAACCATGGATAAGACATGAACTTCCGATGCTTGAAGGTCATATCAAGGTCTGTCTTCCTCTCTCTGCCTCCTTATCACACACACACACACACACACACACACACATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04064 ko04115 ko04210 map04064 map04115 map04210
Pfam Domains
Protein Families
Death LRR_8 Peptidase_S68 ZU5

Protein Analysis

1583

Amino Acids

178.61

Weight (kDa)

6.39

Isoelectric Point (pI)

45.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 140 - 247 7.9e-07 Leucine-rich repeat region
LRR_13 PF23286 169 - 237 1.1e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 222 - 322 7.7e-11 Leucine-rich repeat region
LRR_14 PF23598 428 - 538 5.2e-06 Leucine-rich repeat region
LRR_4 PF12799 438 - 475 1.4e-06 Leucine Rich repeats (2 copies)
LRR_14 PF23598 561 - 676 1.9e-11 Leucine-rich repeat region
LRR_14 PF23598 658 - 756 2.8e-12 Leucine-rich repeat region
LRR_8 PF13855 692 - 748 1.6e-07 Leucine rich repeat
C-JID PF20160 1377 - 1515 7.9e-07 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000307)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g16600 FvH4_1g16610 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16621 FvH4_1g16630 FvH4_1g16640 FvH4_1g16640 FvH4_1g16650 FvH4_1g16650 FvH4_3g27631 FvH4_4g11392 FvH4_4g11542
prunus_persica Prupe.4G224500_v2.0.a1 Prupe.4G226900_v2.0.a1 Prupe.4G227000_v2.0.a1
pyrus_communis pycom11g18180 pycom11g18210 pycom11g18230 pycom11g18270 pycom11g18350 pycom11g18360 pycom11g18370
rosa_chinensis RchiOBHm_Chr2g0106511 RchiOBHm_Chr2g0106541 RchiOBHm_Chr2g0106551 RchiOBHm_Chr2g0106571 RchiOBHm_Chr2g0124621 RchiOBHm_Chr6g0251591 RchiOBHm_Chr6g0251631 RchiOBHm_Chr6g0251641
rosa_laevigata RLG00000015443 RLG00000015444 RLG00000015458 RLG00000017510 RLG00000017511 RLG00000017512 RLG00000017515 RLG00000017516 RLG00000017517 RLG00000018809 RLG00000020934 RLG00000020935 RLG00000034092
rosa_multiflora Rmu_co8285777.1_g000001 Rmu_sc0000762.1_g000001 Rmu_sc0001872.1_g000006 Rmu_sc0005255.1_g000019 Rmu_sc0005597.1_g000006 Rmu_sc0012104.1_g000001 Rmu_sc0017105.1_g000011 Rmu_sc0022904.1_g000001 Rmu_ssc0000135.1_g000049 Rmu_ssc0000213.1_g000047
rosa_roxburghii Rroxscaffold_2G00092830 Rroxscaffold_2G00119790 Rroxscaffold_2G00137010 Rroxscaffold_2G00137030 Rroxscaffold_2G00137070 Rroxscaffold_7G00216560
rosa_rugosa Rorug02G0136100 Rorug02G0136200 Rorug02G0136300 Rorug02G0136400 Rorug02G0251800 Rorug02G0251800 Rorug02G0455500 Rorug02G0455600 Rorug02G0455700 Rorug05G0195300
rosa_samantha Rh2AG187400 Rh2AG187500 Rh2AG187600 Rh2AG310500 Rh2BG198200 Rh2BG198300 Rh2BG198400 Rh2BG198600 Rh2BG318900 Rh2BG534300 Rh2CG192400 Rh2CG192500 Rh2CG192600 Rh2CG297500 Rh2DG193500 Rh2DG193600 Rh2DG193800 Rh2DG334200 Rh2DG543100 Rh5AG280400 Rh5BG285800 Rh5BG285900 Rh5CG317900 Rh5CG318000 Rh5DG294600 Rh5DG294700 Rh6AG012200 Rh6AG012700 Rh6BG011200 Rh6BG011700
rosa_wichuraiana Rw0G009380 Rw2G014610 Rw2G014630 Rw2G014640 Rw2G014680 Rw2G025120 Rw2G043000 Rw5G026490 Rw6G001370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 123
Acc36I ACCTGC 1 cut(s) 2140
AccB7I CCANNNNNTGG 5 cut(s) 445, 646, 1439, 1456, 2807
AccI GTMKAC 2 cut(s) 2185, 4519
AciI CCGC 4 cut(s) 262, 1226, 3772, 4491
AclI AACGTT 1 cut(s) 3912
AcuI CTGAAG 8 cut(s) 133, 134, 340, 1538, 2749, 2841, 3965, 4040
AcvI CACGTG 1 cut(s) 2804
AdeI CACNNNGTG 1 cut(s) 2807
AfaI GTAC 9 cut(s) 1176, 2530, 2617, 3322, 3465, 3727, 3738, 3957, 4532
AflII CTTAAG 2 cut(s) 40, 1385
AflIII ACRYGT 4 cut(s) 1530, 2290, 3460, 3637
AjiI CACGTC 2 cut(s) 3158, 4070
AjnI CCWGG 3 cut(s) 348, 1779, 3660
AjuI GAANNNNNNNTTGG 4 cut(s) 694, 726, 3866, 3898
AleI CACNNNNGTG 2 cut(s) 2521, 4003
Alw21I GWGCWC 2 cut(s) 147, 1754
Alw26I GTCTC 8 cut(s) 163, 1451, 1527, 2605, 2690, 2866, 3965, 4529
Alw44I GTGCAC 1 cut(s) 143
AlwNI CAGNNNCTG 3 cut(s) 1033, 2603, 3617
AoxI GGCC 5 cut(s) 652, 1663, 2578, 3663, 4470
ApaLI GTGCAC 1 cut(s) 143
AseI ATTAAT 1 cut(s) 3531
Asp700I GAANNNNTTC 2 cut(s) 1281, 3167
AspA2I CCTAGG 1 cut(s) 964
AspLEI GCGC 1 cut(s) 3515
AspS9I GGNCC 8 cut(s) 364, 1664, 2476, 2579, 3435, 3547, 3664, 3841
AsuC2I CCSGG 2 cut(s) 2396, 4298
AsuHPI GGTGA 7 cut(s) 410, 1022, 1051, 1421, 2408, 3215, 3990
AsuII TTCGAA 1 cut(s) 517
AvaII GGWCC 5 cut(s) 364, 2476, 3435, 3547, 3841
AvrII CCTAGG 1 cut(s) 964
BaeGI GKGCMC 2 cut(s) 147, 846
BamHI GGATCC 2 cut(s) 902, 3407
BarI GAAGNNNNNNTAC 2 cut(s) 3313, 3345
BauI CACGAG 2 cut(s) 3468, 4223
BbrPI CACGTG 1 cut(s) 2804
BbsI GAAGAC 4 cut(s) 689, 3597, 4173, 4688
Bbv12I GWGCWC 2 cut(s) 147, 1754
BceAI ACGGC 3 cut(s) 627, 1638, 3549
BciT130I CCWGG 3 cut(s) 350, 1781, 3662
BciVI GTATCC 2 cut(s) 574, 2895
BclI TGATCA 3 cut(s) 3274, 3382, 4630
BcnI CCSGG 2 cut(s) 2396, 4298
BcoDI GTCTC 8 cut(s) 163, 1451, 1527, 2605, 2690, 2866, 3965, 4529
BfmI CTRYAG 7 cut(s) 1025, 1690, 1993, 2009, 2252, 2928, 3088
BfrI CTTAAG 2 cut(s) 40, 1385
BfuAI ACCTGC 1 cut(s) 2140
BfuI GTATCC 2 cut(s) 574, 2895
BglII AGATCT 3 cut(s) 509, 944, 1144
BlnI CCTAGG 1 cut(s) 964
Bme1390I CCNGG 5 cut(s) 350, 1781, 2396, 3662, 4298
Bme18I GGWCC 5 cut(s) 364, 2476, 3435, 3547, 3841
BmgBI CACGTC 2 cut(s) 3158, 4070
BmgT120I GGNCC 8 cut(s) 364, 1664, 2476, 2579, 3435, 3547, 3664, 3841
BmiI GGNNCC 8 cut(s) 366, 686, 904, 1377, 1697, 3409, 3436, 3902
BmrFI CCNGG 5 cut(s) 350, 1781, 2396, 3662, 4298
BmrI ACTGGG 5 cut(s) 1497, 1837, 2417, 2591, 2781
BmuI ACTGGG 5 cut(s) 1497, 1837, 2417, 2591, 2781
BoxI GACNNNNGTC 1 cut(s) 4281
BpiI GAAGAC 4 cut(s) 689, 3597, 4173, 4688
BplI GAGNNNNNCTC 4 cut(s) 2186, 2218, 2188, 2220
BpmI CTGGAG 3 cut(s) 3782, 3832, 4258
Bpu14I TTCGAA 1 cut(s) 517
BpuEI CTTGAG 5 cut(s) 395, 848, 2678, 3398, 4339
BpuMI CCSGG 2 cut(s) 2396, 4298
BsaAI YACGTR 4 cut(s) 761, 1178, 2619, 2804
BsaI GGTCTC 1 cut(s) 2866
BsaWI WCCGGW 1 cut(s) 4441
BsaXI ACNNNNNCTCC 2 cut(s) 1948, 1978
Bse1I ACTGG 9 cut(s) 1503, 1843, 2423, 2429, 2597, 2776, 3040, 4145, 4241
Bse3DI GCAATG 4 cut(s) 3004, 3157, 3691, 4217
BseBI CCWGG 3 cut(s) 350, 1781, 3662
BseMI GCAATG 4 cut(s) 3004, 3157, 3691, 4217
BseNI ACTGG 9 cut(s) 1503, 1843, 2423, 2429, 2597, 2776, 3040, 4145, 4241
BseRI GAGGAG 6 cut(s) 821, 851, 1231, 1817, 2708, 4559
BseSI GKGCMC 2 cut(s) 147, 846
BseYI CCCAGC 1 cut(s) 2682
BsgI GTGCAG 2 cut(s) 700, 3714
BshFI GGCC 5 cut(s) 654, 1665, 2580, 3665, 4472
BsiHKAI GWGCWC 2 cut(s) 147, 1754
BsiSI CCGG 5 cut(s) 776, 2395, 2544, 4298, 4442
BslFI GGGAC 5 cut(s) 28, 350, 1633, 2640, 3448
BsmAI GTCTC 8 cut(s) 163, 1451, 1527, 2605, 2690, 2866, 3965, 4529
BsmBI CGTCTC 1 cut(s) 4529
BsmFI GGGAC 5 cut(s) 28, 350, 1633, 2640, 3448
BsmI GAATGC 2 cut(s) 197, 3572
BsnI GGCC 5 cut(s) 654, 1665, 2580, 3665, 4472
Bso31I GGTCTC 1 cut(s) 2866
Bsp119I TTCGAA 1 cut(s) 517
Bsp1286I GDGCHC 3 cut(s) 147, 846, 1754
Bsp1407I TGTACA 3 cut(s) 3320, 3955, 4530
Bsp19I CCATGG 2 cut(s) 889, 4646
BspACI CCGC 4 cut(s) 262, 1226, 3772, 4491
BspANI GGCC 5 cut(s) 654, 1665, 2580, 3665, 4472
BspHI TCATGA 3 cut(s) 2178, 3379, 4273
BspLI GGNNCC 8 cut(s) 366, 686, 904, 1377, 1697, 3409, 3436, 3902
BspMAI CTGCAG 5 cut(s) 1029, 1694, 2013, 2932, 3092
BspMI ACCTGC 1 cut(s) 2140
BspT104I TTCGAA 1 cut(s) 517
BspTI CTTAAG 2 cut(s) 40, 1385
BspTNI GGTCTC 1 cut(s) 2866
BsrDI GCAATG 4 cut(s) 3004, 3157, 3691, 4217
BsrGI TGTACA 3 cut(s) 3320, 3955, 4530
BsrI ACTGG 9 cut(s) 1503, 1843, 2423, 2429, 2597, 2776, 3040, 4145, 4241
BssNAI GTATAC 2 cut(s) 2186, 4520
BssSI CACGAG 2 cut(s) 3468, 4223
BssT1I CCWWGG 6 cut(s) 889, 964, 3209, 4041, 4326, 4646
Bst1107I GTATAC 2 cut(s) 2186, 4520
Bst2BI CACGAG 2 cut(s) 3468, 4223
Bst2UI CCWGG 3 cut(s) 350, 1781, 3662
Bst6I CTCTTC 6 cut(s) 519, 3158, 4004, 4368, 4505, 4536
BstAFI CTTAAG 2 cut(s) 40, 1385
BstAUI TGTACA 3 cut(s) 3320, 3955, 4530
BstBAI YACGTR 4 cut(s) 761, 1178, 2619, 2804
BstBI TTCGAA 1 cut(s) 517
BstC8I GCNNGC 2 cut(s) 47, 3622
BstDSI CCRYGG 3 cut(s) 889, 4001, 4646
BstHHI GCGC 1 cut(s) 3515
BstMAI GTCTC 8 cut(s) 163, 1451, 1527, 2605, 2690, 2866, 3965, 4529
BstMWI GCNNNNNNNGC 5 cut(s) 1090, 1995, 3695, 3749, 4079
BstNI CCWGG 3 cut(s) 350, 1781, 3662
BstNSI RCATGY 4 cut(s) 1534, 2294, 3464, 3641
BstPAI GACNNNNGTC 1 cut(s) 4281
BstSCI CCNGG 5 cut(s) 348, 1779, 2394, 3660, 4296
BstSFI CTRYAG 7 cut(s) 1025, 1690, 1993, 2009, 2252, 2928, 3088
BstSLI GKGCMC 2 cut(s) 147, 846
BstV2I GAAGAC 4 cut(s) 689, 3597, 4173, 4688
BstX2I RGATCY 9 cut(s) 316, 433, 509, 902, 944, 1144, 1356, 3056, 3407
BstXI CCANNNNNNTGG 5 cut(s) 349, 2523, 2559, 3557, 3628
BstYI RGATCY 9 cut(s) 316, 433, 509, 902, 944, 1144, 1356, 3056, 3407
BstZ17I GTATAC 2 cut(s) 2186, 4520
BsuI GTATCC 2 cut(s) 574, 2895
BsuRI GGCC 5 cut(s) 654, 1665, 2580, 3665, 4472
BtgI CCRYGG 3 cut(s) 889, 4001, 4646
BtrI CACGTC 2 cut(s) 3158, 4070
BtsI GCAGTG 2 cut(s) 1022, 4388
BtsIMutI CAGTG 5 cut(s) 728, 1022, 4152, 4388, 4456
BveI ACCTGC 1 cut(s) 2140
Cac8I GCNNGC 2 cut(s) 47, 3622
CaiI CAGNNNCTG 3 cut(s) 1033, 2603, 3617
CciI TCATGA 3 cut(s) 2178, 3379, 4273
CfoI GCGC 1 cut(s) 3515
Cfr13I GGNCC 8 cut(s) 364, 1664, 2476, 2579, 3435, 3547, 3664, 3841
CseI GACGC 1 cut(s) 4271
Csp6I GTAC 9 cut(s) 1175, 2529, 2616, 3321, 3464, 3726, 3737, 3956, 4531
CviQI GTAC 9 cut(s) 1175, 2529, 2616, 3321, 3464, 3726, 3737, 3956, 4531
DraI TTTAAA 3 cut(s) 223, 1170, 2883
DraIII CACNNNGTG 1 cut(s) 2807
Eam1104I CTCTTC 6 cut(s) 519, 3158, 4004, 4368, 4505, 4536
EarI CTCTTC 6 cut(s) 519, 3158, 4004, 4368, 4505, 4536
EciI GGCGGA 1 cut(s) 1215
Eco130I CCWWGG 6 cut(s) 889, 964, 3209, 4041, 4326, 4646
Eco147I AGGCCT 1 cut(s) 654
Eco31I GGTCTC 1 cut(s) 2866
Eco32I GATATC 2 cut(s) 1223, 2653
Eco47I GGWCC 5 cut(s) 364, 2476, 3435, 3547, 3841
Eco57I CTGAAG 8 cut(s) 133, 134, 340, 1538, 2749, 2841, 3965, 4040
Eco72I CACGTG 1 cut(s) 2804
EcoO109I RGGNCCY 2 cut(s) 364, 3435
EcoRI GAATTC 2 cut(s) 1641, 2547
EcoRII CCWGG 3 cut(s) 348, 1779, 3660
EcoRV GATATC 2 cut(s) 1223, 2653
EcoT14I CCWWGG 6 cut(s) 889, 964, 3209, 4041, 4326, 4646
EcoT22I ATGCAT 2 cut(s) 1656, 2353
ErhI CCWWGG 6 cut(s) 889, 964, 3209, 4041, 4326, 4646
Esp3I CGTCTC 1 cut(s) 4529
FalI AAGNNNNNCTT 2 cut(s) 1965, 1997
FaqI GGGAC 5 cut(s) 28, 350, 1633, 2640, 3448
FauNDI CATATG 1 cut(s) 2779
FbaI TGATCA 3 cut(s) 3274, 3382, 4630
FblI GTMKAC 2 cut(s) 2185, 4519
GlaI GCGC 1 cut(s) 3514
GsaI CCCAGC 1 cut(s) 2686
GsuI CTGGAG 3 cut(s) 3782, 3832, 4258
HaeIII GGCC 5 cut(s) 654, 1665, 2580, 3665, 4472
HapII CCGG 5 cut(s) 776, 2395, 2544, 4298, 4442
HgaI GACGC 1 cut(s) 4271
HhaI GCGC 1 cut(s) 3515
Hin6I GCGC 1 cut(s) 3513
HinP1I GCGC 1 cut(s) 3513
HincII GTYRAC 2 cut(s) 3100, 4613
HindII GTYRAC 2 cut(s) 3100, 4613
HindIII AAGCTT 2 cut(s) 43, 371
HpaI GTTAAC 1 cut(s) 3100
HpaII CCGG 5 cut(s) 776, 2395, 2544, 4298, 4442
HphI GGTGA 7 cut(s) 410, 1022, 1051, 1421, 2408, 3215, 3990
HpyF10VI GCNNNNNNNGC 5 cut(s) 1090, 1995, 3695, 3749, 4079
HspAI GCGC 1 cut(s) 3513
Ksp22I TGATCA 3 cut(s) 3274, 3382, 4630
KspAI GTTAAC 1 cut(s) 3100
LmnI GCTCC 4 cut(s) 622, 808, 1804, 3757
MfeI CAATTG 3 cut(s) 1904, 3712, 3765
MflI RGATCY 9 cut(s) 316, 433, 509, 902, 944, 1144, 1356, 3056, 3407
MhlI GDGCHC 3 cut(s) 147, 846, 1754
MlyI GAGTC 5 cut(s) 1039, 2572, 2986, 3830, 3967
MmeI TCCRAC 8 cut(s) 463, 1193, 1359, 1474, 2549, 4238, 4310, 4556
Mph1103I ATGCAT 2 cut(s) 1656, 2353
MroXI GAANNNNTTC 2 cut(s) 1281, 3167
MspA1I CMGCKG 1 cut(s) 3617
MspCI CTTAAG 2 cut(s) 40, 1385
MspI CCGG 5 cut(s) 776, 2395, 2544, 4298, 4442
MspR9I CCNGG 5 cut(s) 350, 1781, 2396, 3662, 4298
MunI CAATTG 3 cut(s) 1904, 3712, 3765
Mva1269I GAATGC 2 cut(s) 197, 3572
MvaI CCWGG 3 cut(s) 350, 1781, 3662
MwoI GCNNNNNNNGC 5 cut(s) 1090, 1995, 3695, 3749, 4079
NciI CCSGG 2 cut(s) 2396, 4298
NcoI CCATGG 2 cut(s) 889, 4646
NdeI CATATG 1 cut(s) 2779
NlaIV GGNNCC 8 cut(s) 366, 686, 904, 1377, 1697, 3409, 3436, 3902
NmuCI GTSAC 6 cut(s) 747, 1599, 2132, 2587, 4313, 4386
NsiI ATGCAT 2 cut(s) 1656, 2353
NspI RCATGY 4 cut(s) 1534, 2294, 3464, 3641
NspV TTCGAA 1 cut(s) 517
OliI CACNNNNGTG 2 cut(s) 2521, 4003
PagI TCATGA 3 cut(s) 2178, 3379, 4273
PceI AGGCCT 1 cut(s) 654
PciI ACATGT 4 cut(s) 1530, 2290, 3460, 3637
PctI GAATGC 2 cut(s) 197, 3572
PdmI GAANNNNTTC 2 cut(s) 1281, 3167
PfeI GAWTC 7 cut(s) 848, 868, 2987, 3493, 4200, 4345, 4404
PflFI GACNNNGTC 1 cut(s) 2773
PflMI CCANNNNNTGG 5 cut(s) 445, 646, 1439, 1456, 2807
PleI GAGTC 5 cut(s) 1038, 2571, 2985, 3829, 3967
PmaCI CACGTG 1 cut(s) 2804
PmlI CACGTG 1 cut(s) 2804
PpsI GAGTC 5 cut(s) 1038, 2571, 2985, 3829, 3967
Ppu21I YACGTR 4 cut(s) 761, 1178, 2619, 2804
PpuMI RGGWCCY 2 cut(s) 364, 3435
PscI ACATGT 4 cut(s) 1530, 2290, 3460, 3637
PshAI GACNNNNGTC 1 cut(s) 4281
PshBI ATTAAT 1 cut(s) 3531
PsiI TTATAA 1 cut(s) 123
Psp1406I AACGTT 1 cut(s) 3912
Psp5II RGGWCCY 2 cut(s) 364, 3435
Psp6I CCWGG 3 cut(s) 348, 1779, 3660
PspCI CACGTG 1 cut(s) 2804
PspFI CCCAGC 1 cut(s) 2682
PspGI CCWGG 3 cut(s) 348, 1779, 3660
PspN4I GGNNCC 8 cut(s) 366, 686, 904, 1377, 1697, 3409, 3436, 3902
PspPI GGNCC 8 cut(s) 364, 1664, 2476, 2579, 3435, 3547, 3664, 3841
PspPPI RGGWCCY 2 cut(s) 364, 3435
PsrI GAACNNNNNNTAC 2 cut(s) 3456, 3488
PstI CTGCAG 5 cut(s) 1029, 1694, 2013, 2932, 3092
PstNI CAGNNNCTG 3 cut(s) 1033, 2603, 3617
PsuI RGATCY 9 cut(s) 316, 433, 509, 902, 944, 1144, 1356, 3056, 3407
PsyI GACNNNGTC 1 cut(s) 2773
PvuII CAGCTG 1 cut(s) 3617
RsaI GTAC 9 cut(s) 1176, 2530, 2617, 3322, 3465, 3727, 3738, 3957, 4532
RsaNI GTAC 9 cut(s) 1175, 2529, 2616, 3321, 3464, 3726, 3737, 3956, 4531
Sau96I GGNCC 8 cut(s) 364, 1664, 2476, 2579, 3435, 3547, 3664, 3841
SchI GAGTC 5 cut(s) 1039, 2572, 2986, 3830, 3967
ScrFI CCNGG 5 cut(s) 350, 1781, 2396, 3662, 4298
SduI GDGCHC 3 cut(s) 147, 846, 1754
SfcI CTRYAG 7 cut(s) 1025, 1690, 1993, 2009, 2252, 2928, 3088
SfuI TTCGAA 1 cut(s) 517
SinI GGWCC 5 cut(s) 364, 2476, 3435, 3547, 3841
SmiI ATTTAAAT 1 cut(s) 1170
SmlI CTYRAG 7 cut(s) 40, 374, 863, 1385, 2657, 3413, 4318
SmoI CTYRAG 7 cut(s) 40, 374, 863, 1385, 2657, 3413, 4318
SseBI AGGCCT 1 cut(s) 654
SsiI CCGC 4 cut(s) 262, 1226, 3772, 4491
SspI AATATT 2 cut(s) 4117, 4639
StuI AGGCCT 1 cut(s) 654
StyD4I CCNGG 5 cut(s) 348, 1779, 2394, 3660, 4296
StyI CCWWGG 6 cut(s) 889, 964, 3209, 4041, 4326, 4646
SwaI ATTTAAAT 1 cut(s) 1170
TaqII GACCGA 2 cut(s) 2120, 2493
TatI WGTACW 6 cut(s) 2528, 3320, 3725, 3736, 3955, 4530
TauI GCSGC 2 cut(s) 264, 3775
TfiI GAWTC 7 cut(s) 848, 868, 2987, 3493, 4200, 4345, 4404
TscAI CASTG 5 cut(s) 735, 1029, 4152, 4395, 4456
TseFI GTSAC 6 cut(s) 747, 1599, 2132, 2587, 4313, 4386
Tsp45I GTSAC 6 cut(s) 747, 1599, 2132, 2587, 4313, 4386
TspGWI ACGGA 5 cut(s) 1140, 1969, 2575, 3420, 4598
TspRI CASTG 5 cut(s) 735, 1029, 4152, 4395, 4456
Tth111I GACNNNGTC 1 cut(s) 2773
Van91I CCANNNNNTGG 5 cut(s) 445, 646, 1439, 1456, 2807
Vha464I CTTAAG 2 cut(s) 40, 1385
VneI GTGCAC 1 cut(s) 143
VpaK11BI GGWCC 5 cut(s) 364, 2476, 3435, 3547, 3841
VspI ATTAAT 1 cut(s) 3531
XbaI TCTAGA 2 cut(s) 478, 1489
XceI RCATGY 4 cut(s) 1534, 2294, 3464, 3641
XcmI CCANNNNNNNNNTGG 1 cut(s) 896
XmaJI CCTAGG 1 cut(s) 964
XmiI GTMKAC 2 cut(s) 2185, 4519
XmnI GAANNNNTTC 2 cut(s) 1281, 3167
Zsp2I ATGCAT 2 cut(s) 1656, 2353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.