Rroxscaffold_2G00137010

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000307
Physical Location & Seq
Forward (+)
74705114 .. 74708451
3338 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00137010.1

Sequence Viewer

Length: 1212 bp
ATGGACACTCTTATCTTAAATAATAGTATAGAATTGTTGTCATCATTTTGCAGGGGAGAATCGGAAGTTATACAGGAAATAGCTAGAGAGATTTCAATATGTTTGAACAACACATTATCCAATCCTGACAGAGATTTGATTGGAATGGATGTGCGTATAGAAAAGTTGGAGTCTTACTTGGATCTAAGGTCGGATGACGTTCTTAGCATAGGGATTTGGGGGATGGGGGGCATTGGTAAGACAACTCTGGCAAAAGAAGTTTTCAAAAAGATTCGTAACCAATTTGATAGGAGCGGCTTTCTTTCCAATGTTAGATTGCATTCCGAAGAAAGAGCTCTAGTTCAGTTGCAAAAACTTCTGTGTGGATCCTCTTTCGAGGACAACAACATAAATATAGACACTGTTGGAGTAGGGATCAGGTTGTTAAAGAAAAGACTACTTAAGAAAAAGGTGCTTATTGTTCTAGATGATGTTGATGAATTAAAACAACTGAAAGATTTAGCACCTGGAAGGCAGAATGAACAGTACTCTTGGGGCCGAGGGAGTCGACTCATAATAACAACTAGGGATAGGCGTCCATTGATAGAATGTGAAGTACATAAAATATATGAGGCTGAAAAACTTAGTGATGAGGAAGCTTCTCAGCTCATATGTCAACAAGCCTTCAAGAAAAACTATCCACCAGCTGAGTTTGTAGAGCTGTCCAAGAGTTTTGTAAAATATGCCGGTGGCCTTCCTCTAGCTCATAAAGTTAAGGGTTCACAATTGTGGGCAAGGGAGGTAGATGAATGGTCAGAGGTATTAGATAGACTGGACGAAGATCTGGATAAAGACATTTTTAGTGTGCTTGAAATAAGTTTTGATGGATTACAGGATACAGATAAGAAAATCTTTTTGGACATCGCATGTTTCTTCAATGGTGAGGATCATGTTCGTGTACAAAAGATATTGAAAAGTTGTGGTTTTTCTCCTCGAATAGGCATAACTAACCTCATTGACAAATCTCTGATCAAAATTGAAAGTGAAAAACTGTGGATGCATGAATTGCTACGGTGTTTGGGTTGGCATATTGTTCGCAGAGAATCAAAACCTTTTCCGGGCAAACGTAGCAGATTGTGGCTTGATGATAATGCACACAAGTACAAAGGCAGAAGGCCATGGCACTTTGAGGATGCTCGTAATGTACTCGCGAGTAATACCGTAAGAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 LRR_8 NB-ARC TIR

Protein Analysis

403

Amino Acids

46.43

Weight (kDa)

6.74

Isoelectric Point (pI)

48.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 53 - 225 1.1e-20 NB-ARC domain
NACHT PF05729 72 - 219 9.4e-08 NACHT domain
WHD_ROQ1 PF23282 293 - 362 2.2e-20 Disease resistance protein Roq1-like, winged-helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000307)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g16600 FvH4_1g16610 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16621 FvH4_1g16630 FvH4_1g16640 FvH4_1g16640 FvH4_1g16650 FvH4_1g16650 FvH4_3g27631 FvH4_4g11392 FvH4_4g11542
prunus_persica Prupe.4G224500_v2.0.a1 Prupe.4G226900_v2.0.a1 Prupe.4G227000_v2.0.a1
pyrus_communis pycom11g18180 pycom11g18210 pycom11g18230 pycom11g18270 pycom11g18350 pycom11g18360 pycom11g18370
rosa_chinensis RchiOBHm_Chr2g0106511 RchiOBHm_Chr2g0106541 RchiOBHm_Chr2g0106551 RchiOBHm_Chr2g0106571 RchiOBHm_Chr2g0124621 RchiOBHm_Chr6g0251591 RchiOBHm_Chr6g0251631 RchiOBHm_Chr6g0251641
rosa_laevigata RLG00000015443 RLG00000015444 RLG00000015458 RLG00000017510 RLG00000017511 RLG00000017512 RLG00000017515 RLG00000017516 RLG00000017517 RLG00000018809 RLG00000020934 RLG00000020935 RLG00000034092
rosa_multiflora Rmu_co8285777.1_g000001 Rmu_sc0000762.1_g000001 Rmu_sc0001872.1_g000006 Rmu_sc0005255.1_g000019 Rmu_sc0005597.1_g000006 Rmu_sc0012104.1_g000001 Rmu_sc0017105.1_g000011 Rmu_sc0022904.1_g000001 Rmu_ssc0000135.1_g000049 Rmu_ssc0000213.1_g000047
rosa_roxburghii Rroxscaffold_2G00092830 Rroxscaffold_2G00119790 Rroxscaffold_2G00137010 Rroxscaffold_2G00137030 Rroxscaffold_2G00137070 Rroxscaffold_7G00216560
rosa_rugosa Rorug02G0136100 Rorug02G0136200 Rorug02G0136300 Rorug02G0136400 Rorug02G0251800 Rorug02G0251800 Rorug02G0455500 Rorug02G0455600 Rorug02G0455700 Rorug05G0195300
rosa_samantha Rh2AG187400 Rh2AG187500 Rh2AG187600 Rh2AG310500 Rh2BG198200 Rh2BG198300 Rh2BG198400 Rh2BG198600 Rh2BG318900 Rh2BG534300 Rh2CG192400 Rh2CG192500 Rh2CG192600 Rh2CG297500 Rh2DG193500 Rh2DG193600 Rh2DG193800 Rh2DG334200 Rh2DG543100 Rh5AG280400 Rh5BG285800 Rh5BG285900 Rh5CG317900 Rh5CG318000 Rh5DG294600 Rh5DG294700 Rh6AG012200 Rh6AG012700 Rh6BG011200 Rh6BG011700
rosa_wichuraiana Rw0G009380 Rw2G014610 Rw2G014630 Rw2G014640 Rw2G014680 Rw2G025120 Rw2G043000 Rw5G026490 Rw6G001370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 294
AccI GTMKAC 1 cut(s) 547
AccII CGCG 1 cut(s) 1190
AciI CCGC 1 cut(s) 294
AclWI GGATC 5 cut(s) 189, 360, 373, 422, 933
AcyI GRCGYC 1 cut(s) 574
AfaI GTAC 5 cut(s) 527, 597, 939, 1142, 1185
AfiI CCNNNNNNNGG 2 cut(s) 977, 1097
AflII CTTAAG 1 cut(s) 440
AgsI TTSAA 8 cut(s) 96, 106, 265, 667, 851, 916, 952, 1019
AjnI CCWGG 1 cut(s) 505
AjuI GAANNNNNNNTTGG 2 cut(s) 878, 910
AleI CACNNNNGTG 1 cut(s) 766
AluBI AGCT 7 cut(s) 83, 335, 638, 646, 686, 700, 743
AluI AGCT 7 cut(s) 83, 335, 638, 646, 686, 700, 743
Alw21I GWGCWC 1 cut(s) 337
AlwI GGATC 5 cut(s) 189, 360, 373, 422, 933
AoxI GGCC 3 cut(s) 535, 730, 1154
AspS9I GGNCC 1 cut(s) 535
AsuC2I CCSGG 1 cut(s) 1098
AsuHPI GGTGA 1 cut(s) 932
BamHI GGATCC 1 cut(s) 365
BanII GRGCYC 1 cut(s) 337
Bbv12I GWGCWC 1 cut(s) 337
BccI CCATC 2 cut(s) 217, 857
BcgI CGANNNNNNTGC 2 cut(s) 1055, 1089
BciT130I CCWGG 1 cut(s) 507
BciVI GTATCC 1 cut(s) 868
BclI TGATCA 1 cut(s) 1008
BcnI CCSGG 1 cut(s) 1098
BfaI CTAG 5 cut(s) 84, 338, 464, 564, 740
BfrI CTTAAG 1 cut(s) 440
BfuI GTATCC 1 cut(s) 868
BglII AGATCT 1 cut(s) 820
BisI GCNGC 1 cut(s) 295
BlsI GCNGC 1 cut(s) 296
BmcAI AGTACT 1 cut(s) 527
Bme1390I CCNGG 2 cut(s) 507, 1098
BmgT120I GGNCC 1 cut(s) 535
BmiI GGNNCC 2 cut(s) 367, 536
BmrFI CCNGG 2 cut(s) 507, 1098
BmsI GCATC 2 cut(s) 1026, 1162
BpuMI CCSGG 1 cut(s) 1098
BsaHI GRCGYC 1 cut(s) 574
BsaJI CCNNGG 2 cut(s) 538, 1157
Bsc4I CCNNNNNNNGG 2 cut(s) 977, 1097
Bse118I RCCGGY 1 cut(s) 725
Bse1I ACTGG 1 cut(s) 816
BseBI CCWGG 1 cut(s) 507
BseDI CCNNGG 2 cut(s) 538, 1157
BseGI GGATG 5 cut(s) 154, 199, 228, 1041, 1177
BseLI CCNNNNNNNGG 2 cut(s) 977, 1097
BseMII CTCAG 2 cut(s) 656, 678
BseNI ACTGG 1 cut(s) 816
BseRI GAGGAG 1 cut(s) 960
Bsh1236I CGCG 1 cut(s) 1190
BshFI GGCC 3 cut(s) 537, 732, 1156
BsiHKAI GWGCWC 1 cut(s) 337
BsiSI CCGG 2 cut(s) 726, 1097
BslI CCNNNNNNNGG 2 cut(s) 977, 1097
BsmI GAATGC 1 cut(s) 319
BsnI GGCC 3 cut(s) 537, 732, 1156
Bsp1286I GDGCHC 1 cut(s) 337
Bsp1407I TGTACA 1 cut(s) 937
Bsp143I GATC 6 cut(s) 181, 365, 414, 820, 925, 1008
Bsp19I CCATGG 1 cut(s) 1157
Bsp68I TCGCGA 1 cut(s) 1190
BspACI CCGC 1 cut(s) 294
BspANI GGCC 3 cut(s) 537, 732, 1156
BspCNI CTCAG 2 cut(s) 655, 679
BspFNI CGCG 1 cut(s) 1190
BspLI GGNNCC 2 cut(s) 367, 536
BspPI GGATC 5 cut(s) 189, 360, 373, 422, 933
BspTI CTTAAG 1 cut(s) 440
BsrBI CCGCTC 1 cut(s) 294
BsrFI RCCGGY 1 cut(s) 725
BsrGI TGTACA 1 cut(s) 937
BsrI ACTGG 1 cut(s) 816
BssAI RCCGGY 1 cut(s) 725
BssECI CCNNGG 2 cut(s) 538, 1157
BssMI GATC 6 cut(s) 181, 365, 414, 820, 925, 1008
BssNI GRCGYC 1 cut(s) 574
BssT1I CCWWGG 1 cut(s) 1157
Bst2UI CCWGG 1 cut(s) 507
Bst4CI ACNGT 5 cut(s) 403, 525, 1032, 1053, 1201
BstACI GRCGYC 1 cut(s) 574
BstAFI CTTAAG 1 cut(s) 440
BstAPI GCANNNNNTGC 1 cut(s) 1045
BstAUI TGTACA 1 cut(s) 937
BstDEI CTNAG 5 cut(s) 185, 203, 623, 642, 687
BstDSI CCRYGG 1 cut(s) 1157
BstENI CCTNNNNNAGG 1 cut(s) 975
BstF5I GGATG 5 cut(s) 154, 199, 228, 1041, 1177
BstFNI CGCG 1 cut(s) 1190
BstKTI GATC 6 cut(s) 184, 368, 417, 823, 928, 1011
BstMBI GATC 6 cut(s) 181, 365, 414, 820, 925, 1008
BstMWI GCNNNNNNNGC 2 cut(s) 1045, 1107
BstNI CCWGG 1 cut(s) 507
BstNSI RCATGY 1 cut(s) 909
BstSCI CCNGG 2 cut(s) 505, 1096
BstUI CGCG 1 cut(s) 1190
BstX2I RGATCY 3 cut(s) 181, 365, 820
BstYI RGATCY 3 cut(s) 181, 365, 820
BsuI GTATCC 1 cut(s) 868
BsuRI GGCC 3 cut(s) 537, 732, 1156
BtgI CCRYGG 1 cut(s) 1157
BtgZI GCGATG 1 cut(s) 886
BtsCI GGATG 5 cut(s) 154, 199, 228, 1041, 1177
BtsIMutI CAGTG 1 cut(s) 399
BtuMI TCGCGA 1 cut(s) 1190
Cfr10I RCCGGY 1 cut(s) 725
Cfr13I GGNCC 1 cut(s) 535
CseI GACGC 1 cut(s) 563
Csp6I GTAC 5 cut(s) 526, 596, 938, 1141, 1184
CviAII CATG 4 cut(s) 906, 929, 1040, 1158
CviQI GTAC 5 cut(s) 526, 596, 938, 1141, 1184
DdeI CTNAG 5 cut(s) 185, 203, 623, 642, 687
DpnI GATC 6 cut(s) 183, 367, 416, 822, 927, 1010
DpnII GATC 6 cut(s) 181, 365, 414, 820, 925, 1008
Ecl136II GAGCTC 1 cut(s) 335
Eco130I CCWWGG 1 cut(s) 1157
Eco24I GRGCYC 1 cut(s) 337
Eco53kI GAGCTC 1 cut(s) 335
EcoICRI GAGCTC 1 cut(s) 335
EcoNI CCTNNNNNAGG 1 cut(s) 975
EcoRII CCWGG 1 cut(s) 505
EcoT14I CCWWGG 1 cut(s) 1157
EcoT22I ATGCAT 1 cut(s) 1041
EcoT38I GRGCYC 1 cut(s) 337
ErhI CCWWGG 1 cut(s) 1157
FaeI CATG 4 cut(s) 909, 932, 1043, 1161
FalI AAGNNNNNCTT 2 cut(s) 875, 907
FatI CATG 4 cut(s) 905, 928, 1039, 1157
FauNDI CATATG 1 cut(s) 650
FbaI TGATCA 1 cut(s) 1008
FblI GTMKAC 1 cut(s) 547
Fnu4HI GCNGC 1 cut(s) 295
FokI GGATG 5 cut(s) 161, 206, 235, 1048, 1184
FriOI GRGCYC 1 cut(s) 337
Fsp4HI GCNGC 1 cut(s) 295
FspBI CTAG 5 cut(s) 84, 338, 464, 564, 740
GluI GCNGC 1 cut(s) 295
HaeIII GGCC 3 cut(s) 537, 732, 1156
HapII CCGG 2 cut(s) 726, 1097
HgaI GACGC 1 cut(s) 563
Hin1I GRCGYC 1 cut(s) 574
Hin1II CATG 4 cut(s) 909, 932, 1043, 1161
HincII GTYRAC 2 cut(s) 548, 656
HindII GTYRAC 2 cut(s) 548, 656
HindIII AAGCTT 1 cut(s) 636
HinfI GANTC 6 cut(s) 59, 170, 271, 544, 549, 1082
HpaII CCGG 2 cut(s) 726, 1097
HphI GGTGA 1 cut(s) 932
Hpy166II GTNNAC 4 cut(s) 548, 656, 761, 938
Hpy188I TCNGA 5 cut(s) 64, 193, 325, 796, 1008
Hpy188III TCNNGA 5 cut(s) 125, 464, 667, 824, 1189
Hpy8I GTNNAC 4 cut(s) 548, 656, 761, 938
HpyAV CCTTC 4 cut(s) 504, 673, 743, 1146
HpyCH4III ACNGT 5 cut(s) 403, 525, 1032, 1053, 1201
HpyCH4IV ACGT 2 cut(s) 198, 1105
HpyCH4V TGCA 5 cut(s) 51, 319, 349, 1039, 1133
HpyF10VI GCNNNNNNNGC 2 cut(s) 1045, 1107
HpyF3I CTNAG 5 cut(s) 185, 203, 623, 642, 687
HpySE526I ACGT 2 cut(s) 198, 1105
Hsp92I GRCGYC 1 cut(s) 574
Hsp92II CATG 4 cut(s) 909, 932, 1043, 1161
Ksp22I TGATCA 1 cut(s) 1008
Kzo9I GATC 6 cut(s) 181, 365, 414, 820, 925, 1008
LmnI GCTCC 1 cut(s) 291
LweI GCATC 2 cut(s) 1026, 1162
MaeI CTAG 5 cut(s) 84, 338, 464, 564, 740
MaeII ACGT 2 cut(s) 198, 1105
MaeIII GTNAC 1 cut(s) 275
MalI GATC 6 cut(s) 183, 367, 416, 822, 927, 1010
MbiI CCGCTC 1 cut(s) 294
MboI GATC 6 cut(s) 181, 365, 414, 820, 925, 1008
MboII GAAGA 3 cut(s) 338, 830, 904
MfeI CAATTG 1 cut(s) 764
MflI RGATCY 3 cut(s) 181, 365, 820
MhlI GDGCHC 1 cut(s) 337
MluCI AATT 6 cut(s) 32, 281, 479, 764, 1014, 1043
MlyI GAGTC 3 cut(s) 179, 543, 553
MmeI TCCRAC 3 cut(s) 147, 171, 385
Mph1103I ATGCAT 1 cut(s) 1041
MseI TTAA 5 cut(s) 17, 425, 441, 482, 753
MslI CAYNNNNRTG 2 cut(s) 766, 933
MspA1I CMGCKG 1 cut(s) 686
MspCI CTTAAG 1 cut(s) 440
MspI CCGG 2 cut(s) 726, 1097
MspR9I CCNGG 2 cut(s) 507, 1098
MunI CAATTG 1 cut(s) 764
Mva1269I GAATGC 1 cut(s) 319
MvaI CCWGG 1 cut(s) 507
MvnI CGCG 1 cut(s) 1190
MwoI GCNNNNNNNGC 2 cut(s) 1045, 1107
NciI CCSGG 1 cut(s) 1098
NcoI CCATGG 1 cut(s) 1157
NdeI CATATG 1 cut(s) 650
NdeII GATC 6 cut(s) 181, 365, 414, 820, 925, 1008
NlaIII CATG 4 cut(s) 909, 932, 1043, 1161
NlaIV GGNNCC 2 cut(s) 367, 536
NmeAIII GCCGAG 1 cut(s) 563
NruI TCGCGA 1 cut(s) 1190
NsiI ATGCAT 1 cut(s) 1041
NspI RCATGY 1 cut(s) 909
OliI CACNNNNGTG 1 cut(s) 766
PctI GAATGC 1 cut(s) 319
PfeI GAWTC 3 cut(s) 59, 271, 1082
PkrI GCNGC 1 cut(s) 296
PleI GAGTC 3 cut(s) 178, 543, 552
PpsI GAGTC 3 cut(s) 178, 543, 552
Psp124BI GAGCTC 1 cut(s) 337
Psp6I CCWGG 1 cut(s) 505
PspGI CCWGG 1 cut(s) 505
PspN4I GGNNCC 2 cut(s) 367, 536
PspPI GGNCC 1 cut(s) 535
PsuI RGATCY 3 cut(s) 181, 365, 820
PvuII CAGCTG 1 cut(s) 686
RruI TCGCGA 1 cut(s) 1190
RsaI GTAC 5 cut(s) 527, 597, 939, 1142, 1185
RsaNI GTAC 5 cut(s) 526, 596, 938, 1141, 1184
RseI CAYNNNNRTG 2 cut(s) 766, 933
SacI GAGCTC 1 cut(s) 337
SalI GTCGAC 1 cut(s) 546
SaqAI TTAA 5 cut(s) 17, 425, 441, 482, 753
SatI GCNGC 1 cut(s) 295
Sau3AI GATC 6 cut(s) 181, 365, 414, 820, 925, 1008
Sau96I GGNCC 1 cut(s) 535
ScaI AGTACT 1 cut(s) 527
SchI GAGTC 3 cut(s) 179, 543, 553
ScrFI CCNGG 2 cut(s) 507, 1098
SduI GDGCHC 1 cut(s) 337
SfaNI GCATC 2 cut(s) 1026, 1162
SmiMI CAYNNNNRTG 2 cut(s) 766, 933
SmlI CTYRAG 1 cut(s) 440
SmoI CTYRAG 1 cut(s) 440
Sse9I AATT 6 cut(s) 32, 281, 479, 764, 1014, 1043
SsiI CCGC 1 cut(s) 294
SspMI CTAG 5 cut(s) 84, 338, 464, 564, 740
SstI GAGCTC 1 cut(s) 337
StyD4I CCNGG 2 cut(s) 505, 1096
StyI CCWWGG 1 cut(s) 1157
TaaI ACNGT 5 cut(s) 403, 525, 1032, 1053, 1201
TaiI ACGT 2 cut(s) 201, 1108
TaqI TCGA 3 cut(s) 375, 547, 973
TasI AATT 6 cut(s) 32, 281, 479, 764, 1014, 1043
TatI WGTACW 5 cut(s) 525, 595, 937, 1140, 1183
TauI GCSGC 1 cut(s) 297
TfiI GAWTC 3 cut(s) 59, 271, 1082
Tru1I TTAA 5 cut(s) 17, 425, 441, 482, 753
Tru9I TTAA 5 cut(s) 17, 425, 441, 482, 753
TscAI CASTG 1 cut(s) 406
TspDTI ATGAA 4 cut(s) 492, 534, 801, 1056
TspRI CASTG 1 cut(s) 406
Vha464I CTTAAG 1 cut(s) 440
XagI CCTNNNNNAGG 1 cut(s) 975
XbaI TCTAGA 1 cut(s) 463
XceI RCATGY 1 cut(s) 909
XmiI GTMKAC 1 cut(s) 547
XspI CTAG 5 cut(s) 84, 338, 464, 564, 740
ZrmI AGTACT 1 cut(s) 527
Zsp2I ATGCAT 1 cut(s) 1041
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.