Rroxscaffold_2G00137030

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000307
Physical Location & Seq
Forward (+)
74728070 .. 74737075
9006 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00137030.1

Sequence Viewer

Length: 5874 bp
ATGCATCAGAGAGGAATCGACACATTTCGAGATACTGAAAAGCTTCAGAGGGGGAAATCCATTTCACCAGAACTTCTCAAAGCAATCGAAGAGTCCAAGTTTGCAGTCACTGTTCTTTCAACCAACTATGCTACTTCAACTTGGTGTCTCGATGAACTTGCACATATTCTTGAAGGCAAGAAACTGAGGGGACTGGAAGTTCTTCCAGTCTTTTATCATGTAGAACCATCTGAGATAAGAAAGCAAACTGGAATATATGGCAAGGCGTTTGCTAAGCACGAGACAGATTTCAAGGACAACATCAAGAAGGTGAACAAGTGGAGGGAAGCTTTAAAGGAGATAGCCAGTCTCTCAGGATGGCATGTGACGAAAGATAGTGGAGAATCAGAAGTTATTCAGGATATAGCTAAAAGGATTTCAAACATATTGAACAACATGTTATCCAATCCTGACAGAGATTTAATTGGAATGGATGAACGCATAGAAAAAATGAAGTCTTACTTGGATCTAAGGTCGGATGATATTTGTACCATAGGGATTTGGGGGATGGGGGGCATAGGTAAGACAACTCTGGCAAAAGAAGTTTTCAAAAAGATTCGTAACCAATTTGATACTAGCGGCTTTGTTTCTGAAGTTAGATTGCATTCGGAAGGAAAAGCTCTAGTAGAGTTGCAAAAGCTTCTCTGTGGATCCTTTTTTGGGGACAGCAATATAAATATAGACACTGTTGGAAGAGGGATCAGGTTGTTGAAGAAGGTATTGCGTAAGAAAAAGGTGCTTATCGTTCTAGACGATGTTGATGAATTAAAACAACTGAAAGACTTAGCACCTGGAAAGCACGATGAAGAGAATTCTTGGGGTCCAGGGAGTCGCCTCATGATAACAACTAGAGATAGGCGCACATTAACAGAGTGCGGAGTACTAGAAGATAAAATATATGAGGTTGAAAAACTGAGTGATGCAGAAGCTTTTCAGCTCATGTCTCAGAAAGCCTTCAAGGAAAACTATCCTCCGAGTGAGTATGTAGAGCTGTTGCAGAGTTTTGTACAATATGCTAGTGGTCTTCCTTTAGCTCATAAAGTACTAGGATCATACTTGTCTGGAAGAAATATAAATGAATGGTCAGAGGCATTGGATAGACTTGATGAAGATCCGGATAAAGACATTTTTAGTGTGCTTCAAATAAGTTTTGATGGATTGCGGGATACAGATAAGAAAATATTTTTGGACATTGCATGTTTCTTCAATGGCGAGGATCATGTCCGTGTAAAAAATATATTGAAAGGTTGTGGCTTTTGTCCTGAAATCGGCATAACTGACCTCATTAATAAATCTCTGATTAAAATTGAAAGAAAAAAACTGTGGATGCATGATTTACTACAACAATTGGGTTGGCATATTGTTCGTCGAGAATCTCCTTTCCCAGGCAATCGTAGCAGGTTGTGGCTTAATGATGGTACCAACAAGTACGAAGGCAGCAGGTCATGGCGTGTTGCGGATACTCGTGATGCACTCACGGAAAATACAGGAACAGCTGCTGTTGAAGGCTTATTCTTAAACTTGCTTGAAAAAGAAGAAATGTGCTTGACTAGTGACCCGTTCTTAAAGATGCGCAACCTAAGACTGTTGAAGATTTGCAATGTGAACTTTCTTAATGTGAACTTTACATATCTCTCTAAAAGACTACGGTTTTTGGAATGGCATGAATGTCCTCTAGAAATTTTATCATCTTGTTTTGAACCAGATCAACTTGTTGAATTCAAGATGCCTAACAGCCACATTAAACACCTATGGAATGAAAGGCTTTATCTAGAAATGCTATTATGCATGGATCTTTCAGACTGCCAATATTTAACCAAGACCCTGGACTTCAGTAAGGTCCCAAAGCTTCAGAGATTGATCCTTAAAGGTTGTAAAGCGTTATTGGAGGTTCACCAAACAATTGGGGATCTCAAACATTTGGTTTTTTTGAACTTGAAAGGTTGCGAAAGTCTAGAAAACCTTCCTCAATCCATCAGGTTGAGATCTCTTCAAACTTTTATTCTTTCGGGATGTTCAAAACTCAGAAACCTTCCAGACATTGTGGGGAACATGGATACTTTATCAGAACTTTGTTTAGATGGCACGGCTATATTGGAGCTACCTGTTTCAATCCAGCGTTTGGGAGGCCTTACTTTGCTAAATCTAAGCAGCTGCAAGAACCTTCTGAGTCTTCCCAGTGTCCTTTGTAGTAGTTTGACATCACTGAAATTTCTCTATCTGTCATTGTGCTCAAGTATGGAAGAACTGCCAGAAAACATAGGCTGCTTGAAGCACTTGGAGGAGCTCGATGTATGTGATACTGCTATCAAAAAAGTGCCCAATTCAATTTCAGGCCTTGAGAATCTTAAACTTTTGTGTTTCCATGGATGCTCTAGATCCGTAGGTTTAGAACTGCCAAACTTGTTCTCAGGTCTAAGATCTTTGACAACACTAAACCTAGGCAGATGCAACCTTGCAGAAGGAGCAATCCCGGGTGACATTGGCGAATTAAGCTCACTGCAGAGTTTGGATTTAAGTGAAAACAACTTTTCGACTATACCTCAGAGCATTTCTAAACTTTCTGAGCTTACAGAAATTTCTTTGTTTAGGTGTAGCAATCTACAGTCGTTGCCAGAAGAATTTCCATCGAGTCTAAGAAATGTAAACATATGTTCTTGTCATATGCCGACAAATTCCTCAGATGGTTGGAGGAGATGGGCTTCTCAAAAGGGTTTGAGTATCATAAATTGTCGAAAACCAGAGGAGGATGAACATTTGTCAAAGCTGCCTGAACTCCCTAAGTTACCTAACTGGTTCTCGAATTTAAGATCTTTGAGATCATTAAATCTACATAGGTGTAGTAAGCTAAAGTCACTGCCAAGACATCTTCCATTCAATCTAAAACATGTCAATGCACGAGAATGTCCCATGTTGAAGAATAATGCAGATACTTTGACAATATGGGCTTCCGGGAAACGGTTCTGTTTCATAAATTGTGGACAATCAGACCAGGATAATGATCAGCCAAGCCACGCTCCAGTTCCGGAAGACAGTATTGGACTACTCTTTTCTAAATATATTCAGGATCGAATCTATGGTAAAAAACCATTTGAATTTCGTTTTCCACATAGTGCAAGGATTCCCCGATTTTGGAGCCATTGGAGGAGTGGCCCTTCTGTGGCAGTCCCGCTATCTGATAGTAACGGTTCGTGGATTGGACTTGCTCTATTTGTTGTTTTCGAAATCCTTGAGATAGAAAAATTCGACAACAGATGGGAATCAGAGGAGACTATCTGTGAATTTCACACCAGTTTTGGGCGTGAAAATTCACTAGTTTTTCAGAACTTCCTAGGCTTCAGGACTGGTTCATATGGACTTTGTTGCTACGAACCACGAGGTGGGCAATTTGGTGGGTTGTTTGATGAACCACTACTTCAAGCTTCAGTTTCAACAAAGAGACCAGATTTAAAAGTGAGAGGTTGTGGGTTGCATCTTTTATCAGAGCATGATGCTGCAGCGTTTGTTCAAAATTTAATAAACCAGACTGCTATTCAACACCTGGATTTCAATTTTGATCGACATTGTGAGGAAATATTAGAAAAGGCAACGATTTGTGACCTGATAGAGCTAGACCCTGATGATCTATGGGAACTAGGATCTACTTCAACTGTGAATGAAGATGACTGCCGCATGAGTAGCTGTAAAATCCAACTCAGAGCACAGCTGTCAATCCTCTATGAGGGACGCAATGGATGTGAGAAGAGTTTCCATGTTTGTCTTCCTGATTCACCAAATGTAATGTCAATTCCGACCTGGTTCATCAATCGCCATGCAGGTGATGTAACACTATGTTACATTAGTGAAAACTTGTTTGCTGATCAGAGATGGGTGGGATTAGAACTATATGTTGTATTTGCCCGGCGGACACCTGCTCCAAGTGATGGCCATATTAATAGTTTCTTCTTTCATGTTGAGTTGTGTGGTCATGATAATGGAAGTCTAATAATGCATGGATCCCTCAAGAGAGAGAGTTGTCTTGGAGAGTCAAATCAACTTCTTGTATTACATTTACCACGCGTTTATTTTCAAGAACAGTTAAATCAATGTCAGGTTATTAGCGCATTGTTTACAACCAGTACCCCAGAATTGGAGGTTATAGTGTGTGGGAGCCGTTTAGTTTATCAGCAAGATTTGAAAGACTTGATCCATTCATTGACTGCGGCTGCAAGCACTTTTGGGGAACATGTCCTCACTCAACTTTGTTCCCAGGCCTGCACCCAAACTGATGATCAGATCATTGCAGTGAAAGGAGAAATGGCGATCAATTGCGATTCCTCATTTCAAAGCAGAAGTACAGCACAATTACCAGAACAAGTTCTTACTTCAATAATGCGGCTGAAAAGTTATAGATGCTATCTTCGGCAAAAGCAAGTTTTTGTAGAGTCTGAACGTACCCCATTAGTTCACTCCAGATCAGTGATACCAGTCCATGCCAAAGGCTGTCTTTTGGAATACAGGGAAAGCAACTCAGAAAATGTAGCAGAAGAAGACACAAAGTCGTCATTGGCTTATTGTCATCATTTACGTATAATGGCTGAGACTCAACTTTACGGAAGCTATAGTTCATTTCAGTTGAAAACATCCCTCCAGTTGTTACTTCAACACTCCAAGGTGGCCACTCTCAGTCTTGGTGGACATACAATTTCTTCTCTCAAGAATTTTGATCCTTCCTCCGCATGCAATATTATTTGTTTCTCTGACAGGGAAATTCCAGTGTGGTTCAAACATGAGATGTCTTATCAACTGTCTTCTAGGTCCAGGGTGGGAATCAAACTACCTCCAAAATTGCAGGAGGATGGGAATTGGAGAGGACTTGCTATATGTGTTGCCTTTGAAGTTCATGACCAGCATCCAACTACCTCCCCGGTCAAACTTCTCTGTCACTTGAGAGCCAAGGACAGCTATTGCTTGAATCCTATCCCTATGTGTTCAATCACTGAAGAGAAACTCAAGTCGTTGCATGTTGTTAGATTCATTTGGCTAAGCTACATCCCCCGTGTTTTGCTAACGGAGTTCAATGTGGTTAGTGATGTAGAGGCCAGAATTTATGTTGGTTGCAAAGGCTTGACAGTGGAGAAGTGTGGTATGCGTCTCTTGTACCGGCAAGAAGAGGGGGAGTTTGAGAGTGCGATAACTGAGTGCTGGACCTCTTTCTTTGATAATCTGTCTTTCATCCGTAGACTTGTAGAAGCAGATGATCAAAATGTTCAACCATGGACAAGGCATGAACTTCCAATGCTTGAAGGGCATATCAAGGTCTTTGATCCGGATATACTATATAATGCAATTCCCACTTCTACTGAAATTCCGAAGTGGTTCGGGCATCCCATTAACGCCAAGGACGATTACGCCATGAACTGGTATGAATACCAGTTACCACCATCTTTGAGTGACAAAAACTGGATAGGATTGGCTATCTGTGCATCACATGGTATTAGCAGAGAGTATTTGGTGGAACGTGATTCCTACCCCTTTATTTTAACATTGAAAACTGGAGACAATGGCTTGTCATCTCTCCATCGGTATCAGATGACCAATGAAGAATCTGAGTTCCTAAAGCGTTGTTGTAATGTGCATGGTGAATTCATTTGGCTCTCCTACATACCACGACGCTGGTTTCTACATCAGCTAAATGACGAGTCTGTCCTCATTGTTTCATCTAGTGAGAACAGCTGGAAGCCGCGCACGGTATATCTCCGTTTTGTGTATGCGCATGAGGTGGAAGAGTTTAAGCAGCTCTGTTTCAATCTTCATCGACCCCCTGCCTGCCCAACAGTAAAAAGATCAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 LRR_8 NB-ARC TIR

Protein Analysis

1957

Amino Acids

222.91

Weight (kDa)

6.24

Isoelectric Point (pI)

48.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 2 - 150 9.8e-44 TIR domain
TIR_2 PF13676 3 - 79 6.9e-10 TIR domain
NB-ARC PF00931 159 - 335 6.5e-22 NB-ARC domain
NACHT PF05729 179 - 329 1.8e-07 NACHT domain
WHD_ROQ1 PF23282 403 - 472 1.1e-18 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 722 - 942 6.9e-18 Leucine-rich repeat region
C-JID PF20160 1593 - 1730 5.2e-07 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000307)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g16600 FvH4_1g16610 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16621 FvH4_1g16630 FvH4_1g16640 FvH4_1g16640 FvH4_1g16650 FvH4_1g16650 FvH4_3g27631 FvH4_4g11392 FvH4_4g11542
prunus_persica Prupe.4G224500_v2.0.a1 Prupe.4G226900_v2.0.a1 Prupe.4G227000_v2.0.a1
pyrus_communis pycom11g18180 pycom11g18210 pycom11g18230 pycom11g18270 pycom11g18350 pycom11g18360 pycom11g18370
rosa_chinensis RchiOBHm_Chr2g0106511 RchiOBHm_Chr2g0106541 RchiOBHm_Chr2g0106551 RchiOBHm_Chr2g0106571 RchiOBHm_Chr2g0124621 RchiOBHm_Chr6g0251591 RchiOBHm_Chr6g0251631 RchiOBHm_Chr6g0251641
rosa_laevigata RLG00000015443 RLG00000015444 RLG00000015458 RLG00000017510 RLG00000017511 RLG00000017512 RLG00000017515 RLG00000017516 RLG00000017517 RLG00000018809 RLG00000020934 RLG00000020935 RLG00000034092
rosa_multiflora Rmu_co8285777.1_g000001 Rmu_sc0000762.1_g000001 Rmu_sc0001872.1_g000006 Rmu_sc0005255.1_g000019 Rmu_sc0005597.1_g000006 Rmu_sc0012104.1_g000001 Rmu_sc0017105.1_g000011 Rmu_sc0022904.1_g000001 Rmu_ssc0000135.1_g000049 Rmu_ssc0000213.1_g000047
rosa_roxburghii Rroxscaffold_2G00092830 Rroxscaffold_2G00119790 Rroxscaffold_2G00137010 Rroxscaffold_2G00137030 Rroxscaffold_2G00137070 Rroxscaffold_7G00216560
rosa_rugosa Rorug02G0136100 Rorug02G0136200 Rorug02G0136300 Rorug02G0136400 Rorug02G0251800 Rorug02G0251800 Rorug02G0455500 Rorug02G0455600 Rorug02G0455700 Rorug05G0195300
rosa_samantha Rh2AG187400 Rh2AG187500 Rh2AG187600 Rh2AG310500 Rh2BG198200 Rh2BG198300 Rh2BG198400 Rh2BG198600 Rh2BG318900 Rh2BG534300 Rh2CG192400 Rh2CG192500 Rh2CG192600 Rh2CG297500 Rh2DG193500 Rh2DG193600 Rh2DG193800 Rh2DG334200 Rh2DG543100 Rh5AG280400 Rh5BG285800 Rh5BG285900 Rh5CG317900 Rh5CG318000 Rh5DG294600 Rh5DG294700 Rh6AG012200 Rh6AG012700 Rh6BG011200 Rh6BG011700
rosa_wichuraiana Rw0G009380 Rw2G014610 Rw2G014630 Rw2G014640 Rw2G014680 Rw2G025120 Rw2G043000 Rw5G026490 Rw6G001370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 3845, 3958
AasI GACNNNNNNGTC 1 cut(s) 5723
Acc16I TGCGCA 2 cut(s) 1613, 5793
Acc36I ACCTGC 4 cut(s) 1428, 1470, 3845, 3958
Acc65I GGTACC 1 cut(s) 1457
AccB1I GGYRCC 1 cut(s) 1457
AccB7I CCANNNNNTGG 4 cut(s) 2161, 3419, 3962, 5439
AccI GTMKAC 1 cut(s) 5260
AccII CGCG 2 cut(s) 4098, 5764
AccIII TCCGGA 3 cut(s) 1153, 3064, 5347
AcoI YGGCCR 2 cut(s) 3964, 4695
AcuI CTGAAG 7 cut(s) 29, 651, 1855, 1874, 3361, 3447, 5040
AdeI CACNNNGTG 2 cut(s) 3152, 3419
AflIII ACRYGT 4 cut(s) 435, 2926, 4096, 4264
AhdI GACNNNNNGTC 1 cut(s) 4576
AhlI ACTAGT 2 cut(s) 1589, 3352
AjnI CCWGG 9 cut(s) 829, 862, 1423, 1863, 3030, 3579, 3833, 4287, 4838
AjuI GAANNNNNNNTTGG 6 cut(s) 485, 517, 1208, 1240, 3060, 3092
AloI GAACNNNNNNTCC 2 cut(s) 183, 215
Alw21I GWGCWC 3 cut(s) 2273, 2328, 3742
Alw26I GTCTC 9 cut(s) 152, 275, 353, 987, 3302, 3472, 4613, 5177, 5571
AlwNI CAGNNNCTG 2 cut(s) 110, 1538
Ama87I CYCGRG 1 cut(s) 2512
Aor13HI TCCGGA 3 cut(s) 1153, 3064, 5347
AoxI GGCC 7 cut(s) 2167, 2374, 3190, 3964, 4290, 4695, 5118
ArsI GACNNNNNNTTYG 2 cut(s) 3806, 3838
AseI ATTAAT 2 cut(s) 1326, 3972
Asp718I GGTACC 1 cut(s) 1457
AspA2I CCTAGG 2 cut(s) 2479, 3370
AspLEI GCGC 5 cut(s) 900, 1614, 4142, 5766, 5794
AspS9I GGNCC 5 cut(s) 860, 1879, 3191, 4836, 5226
AsuC2I CCSGG 5 cut(s) 2513, 2514, 2992, 3940, 4946
AsuHPI GGTGA 7 cut(s) 57, 322, 1925, 2528, 3801, 3869, 5672
AsuII TTCGAA 1 cut(s) 3261
AvaI CYCGRG 1 cut(s) 2512
AvaII GGWCC 4 cut(s) 860, 1879, 4836, 5226
AvrII CCTAGG 2 cut(s) 2479, 3370
BaeGI GKGCMC 1 cut(s) 2361
BalI TGGCCA 2 cut(s) 3966, 4697
BamHI GGATCC 2 cut(s) 689, 4034
BanI GGYRCC 1 cut(s) 1457
BanII GRGCYC 1 cut(s) 2328
BauI CACGAG 4 cut(s) 278, 1503, 2937, 3414
BbsI GAAGAC 6 cut(s) 1055, 2204, 3075, 3791, 4575, 4821
Bbv12I GWGCWC 3 cut(s) 2273, 2328, 3742
BceAI ACGGC 2 cut(s) 2142, 4176
BcgI CGANNNNNNTGC 4 cut(s) 140, 174, 1385, 1419
BciT130I CCWGG 9 cut(s) 831, 864, 1425, 1865, 3032, 3581, 3835, 4289, 4840
BciVI GTATCC 3 cut(s) 1198, 1492, 2089
BclI TGATCA 4 cut(s) 3040, 3898, 4309, 5278
BcnI CCSGG 5 cut(s) 2513, 2514, 2992, 3940, 4946
BcoDI GTCTC 9 cut(s) 152, 275, 353, 987, 3302, 3472, 4613, 5177, 5571
BcuI ACTAGT 2 cut(s) 1589, 3352
BfmI CTRYAG 4 cut(s) 2540, 2642, 3534, 4639
BfuAI ACCTGC 4 cut(s) 1428, 1470, 3845, 3958
BfuI GTATCC 3 cut(s) 1198, 1492, 2089
BglII AGATCT 3 cut(s) 2024, 2459, 2849
BlnI CCTAGG 2 cut(s) 2479, 3370
BlpI GCTNAGC 2 cut(s) 273, 5063
BmcAI AGTACT 2 cut(s) 921, 1083
Bme18I GGWCC 4 cut(s) 860, 1879, 4836, 5226
BmeRI GACNNNNNGTC 1 cut(s) 4576
BmeT110I CYCGRG 1 cut(s) 2512
BmgT120I GGNCC 5 cut(s) 860, 1879, 3191, 4836, 5226
BmiI GGNNCC 7 cut(s) 691, 861, 1459, 1881, 3176, 4036, 4190
BmrI ACTGGG 1 cut(s) 2211
BmuI ACTGGG 1 cut(s) 2211
BpiI GAAGAC 6 cut(s) 1055, 2204, 3075, 3791, 4575, 4821
BpmI CTGGAG 4 cut(s) 3042, 4474, 4652, 5595
Bpu1102I GCTNAGC 2 cut(s) 273, 5063
Bpu14I TTCGAA 1 cut(s) 3261
BpuEI CTTGAG 7 cut(s) 2257, 2399, 3290, 4025, 4718, 4987, 5015
BpuMI CCSGG 5 cut(s) 2513, 2514, 2992, 3940, 4946
BsaAI YACGTR 1 cut(s) 4607
BsaBI GATNNNNATC 3 cut(s) 1149, 3039, 3298
BsaI GGTCTC 1 cut(s) 3472
BsaWI WCCGGW 3 cut(s) 1153, 3064, 5347
BsaXI ACNNNNNCTCC 2 cut(s) 3937, 3967
Bse118I RCCGGY 1 cut(s) 5181
Bse3DI GCAATG 4 cut(s) 1230, 1645, 3775, 4317
Bse8I GATNNNNATC 3 cut(s) 1149, 3039, 3298
BseAI TCCGGA 3 cut(s) 1153, 3064, 5347
BseBI CCWGG 9 cut(s) 831, 864, 1425, 1865, 3032, 3581, 3835, 4289, 4840
BseJI GATNNNNATC 3 cut(s) 1149, 3039, 3298
BseMI GCAATG 4 cut(s) 1230, 1645, 3775, 4317
BseRI GAGGAG 5 cut(s) 2336, 2746, 2798, 3199, 3320
BseSI GKGCMC 1 cut(s) 2361
BsgI GTGCAG 1 cut(s) 4279
Bsh1236I CGCG 2 cut(s) 4098, 5764
BshFI GGCC 7 cut(s) 2169, 2376, 3192, 3966, 4292, 4697, 5120
BshNI GGYRCC 1 cut(s) 1457
BsiHKAI GWGCWC 3 cut(s) 2273, 2328, 3742
BsiHKCI CYCGRG 1 cut(s) 2512
BsiSI CCGG 8 cut(s) 1154, 2513, 2991, 3065, 3940, 4946, 5182, 5348
BslFI GGGAC 6 cut(s) 204, 716, 1865, 2931, 3191, 3777
BsmAI GTCTC 9 cut(s) 152, 275, 353, 987, 3302, 3472, 4613, 5177, 5571
BsmBI CGTCTC 1 cut(s) 5177
BsmFI GGGAC 6 cut(s) 204, 716, 1865, 2931, 3191, 3777
BsmI GAATGC 1 cut(s) 643
BsnI GGCC 7 cut(s) 2169, 2376, 3192, 3966, 4292, 4697, 5120
Bso31I GGTCTC 1 cut(s) 3472
BsoBI CYCGRG 1 cut(s) 2512
Bsp119I TTCGAA 1 cut(s) 3261
Bsp1286I GDGCHC 4 cut(s) 2273, 2328, 2361, 3742
Bsp13I TCCGGA 3 cut(s) 1153, 3064, 5347
Bsp1407I TGTACA 1 cut(s) 1045
Bsp1720I GCTNAGC 2 cut(s) 273, 5063
Bsp19I CCATGG 2 cut(s) 2404, 5294
BspANI GGCC 7 cut(s) 2169, 2376, 3192, 3966, 4292, 4697, 5120
BspEI TCCGGA 3 cut(s) 1153, 3064, 5347
BspFNI CGCG 2 cut(s) 4098, 5764
BspHI TCATGA 3 cut(s) 876, 4006, 4921
BspLI GGNNCC 7 cut(s) 691, 861, 1459, 1881, 3176, 4036, 4190
BspMAI CTGCAG 2 cut(s) 2544, 3538
BspMI ACCTGC 4 cut(s) 1428, 1470, 3845, 3958
BspT104I TTCGAA 1 cut(s) 3261
BspT107I GGYRCC 1 cut(s) 1457
BspTNI GGTCTC 1 cut(s) 3472
BsrDI GCAATG 4 cut(s) 1230, 1645, 3775, 4317
BsrFI RCCGGY 1 cut(s) 5181
BsrGI TGTACA 1 cut(s) 1045
BssAI RCCGGY 1 cut(s) 5181
BssSI CACGAG 4 cut(s) 278, 1503, 2937, 3414
BssT1I CCWWGG 7 cut(s) 2404, 2479, 3370, 4689, 4974, 5294, 5418
Bst2BI CACGAG 4 cut(s) 278, 1503, 2937, 3414
Bst2UI CCWGG 9 cut(s) 831, 864, 1425, 1865, 3032, 3581, 3835, 4289, 4840
Bst6I CTCTTC 8 cut(s) 84, 727, 840, 2034, 3776, 5016, 5184, 5799
BstAUI TGTACA 1 cut(s) 1045
BstBAI YACGTR 1 cut(s) 4607
BstBI TTCGAA 1 cut(s) 3261
BstC8I GCNNGC 4 cut(s) 4249, 4294, 4759, 5848
BstDSI CCRYGG 2 cut(s) 2404, 5294
BstENI CCTNNNNNAGG 2 cut(s) 1422, 3758
BstFNI CGCG 2 cut(s) 4098, 5764
BstHHI GCGC 5 cut(s) 900, 1614, 4142, 5766, 5794
BstMAI GTCTC 9 cut(s) 152, 275, 353, 987, 3302, 3472, 4613, 5177, 5571
BstMWI GCNNNNNNNGC 6 cut(s) 1434, 2504, 2532, 3717, 5326, 5501
BstNI CCWGG 9 cut(s) 831, 864, 1425, 1865, 3032, 3581, 3835, 4289, 4840
BstNSI RCATGY 7 cut(s) 365, 439, 1239, 2930, 4268, 4761, 5045
BstSFI CTRYAG 4 cut(s) 2540, 2642, 3534, 4639
BstSLI GKGCMC 1 cut(s) 2361
BstSNI TACGTA 1 cut(s) 4607
BstUI CGCG 2 cut(s) 4098, 5764
BstV2I GAAGAC 6 cut(s) 1055, 2204, 3075, 3791, 4575, 4821
BstXI CCANNNNNNTGG 3 cut(s) 1864, 1943, 5694
BsuI GTATCC 3 cut(s) 1198, 1492, 2089
BsuRI GGCC 7 cut(s) 2169, 2376, 3192, 3966, 4292, 4697, 5120
BtgI CCRYGG 2 cut(s) 2404, 5294
BtsI GCAGTG 3 cut(s) 2537, 2894, 4329
BveI ACCTGC 4 cut(s) 1428, 1470, 3845, 3958
Cac8I GCNNGC 4 cut(s) 4249, 4294, 4759, 5848
CaiI CAGNNNCTG 2 cut(s) 110, 1538
CciI TCATGA 3 cut(s) 876, 4006, 4921
CfoI GCGC 5 cut(s) 900, 1614, 4142, 5766, 5794
Cfr10I RCCGGY 1 cut(s) 5181
Cfr13I GGNCC 5 cut(s) 860, 1879, 3191, 4836, 5226
Cfr9I CCCGGG 1 cut(s) 2512
CseI GACGC 3 cut(s) 3774, 5159, 5700
CsiI ACCWGGT 1 cut(s) 3833
CspCI CAANNNNNGTGG 2 cut(s) 5448, 5483
DraI TTTAAA 2 cut(s) 333, 3489
DraIII CACNNNGTG 2 cut(s) 3152, 3419
DrdI GACNNNNNNGTC 1 cut(s) 5723
DriI GACNNNNNGTC 1 cut(s) 4576
DseDI GACNNNNNNGTC 1 cut(s) 5723
EaeI YGGCCR 2 cut(s) 3964, 4695
Eam1104I CTCTTC 8 cut(s) 84, 727, 840, 2034, 3776, 5016, 5184, 5799
Eam1105I GACNNNNNGTC 1 cut(s) 4576
EarI CTCTTC 8 cut(s) 84, 727, 840, 2034, 3776, 5016, 5184, 5799
EciI GGCGGA 1 cut(s) 3958
Ecl136II GAGCTC 1 cut(s) 2326
Eco105I TACGTA 1 cut(s) 4607
Eco130I CCWWGG 7 cut(s) 2404, 2479, 3370, 4689, 4974, 5294, 5418
Eco147I AGGCCT 3 cut(s) 2169, 2376, 4292
Eco24I GRGCYC 1 cut(s) 2328
Eco31I GGTCTC 1 cut(s) 3472
Eco47I GGWCC 4 cut(s) 860, 1879, 4836, 5226
Eco53kI GAGCTC 1 cut(s) 2326
Eco57I CTGAAG 7 cut(s) 29, 651, 1855, 1874, 3361, 3447, 5040
Eco88I CYCGRG 1 cut(s) 2512
EcoICRI GAGCTC 1 cut(s) 2326
EcoNI CCTNNNNNAGG 2 cut(s) 1422, 3758
EcoO109I RGGNCCY 1 cut(s) 1879
EcoRI GAATTC 3 cut(s) 850, 1757, 5663
EcoRII CCWGG 9 cut(s) 829, 862, 1423, 1863, 3030, 3579, 3833, 4287, 4838
EcoT14I CCWWGG 7 cut(s) 2404, 2479, 3370, 4689, 4974, 5294, 5418
EcoT22I ATGCAT 4 cut(s) 6, 1371, 1829, 4032
EcoT38I GRGCYC 1 cut(s) 2328
ErhI CCWWGG 7 cut(s) 2404, 2479, 3370, 4689, 4974, 5294, 5418
Esp3I CGTCTC 1 cut(s) 5177
FalI AAGNNNNNCTT 4 cut(s) 485, 517, 4509, 4541
FaqI GGGAC 6 cut(s) 204, 716, 1865, 2931, 3191, 3777
FauI CCCGC 2 cut(s) 1194, 3216
FauNDI CATATG 3 cut(s) 2690, 2703, 3391
FbaI TGATCA 4 cut(s) 3040, 3898, 4309, 5278
FblI GTMKAC 1 cut(s) 5260
FriOI GRGCYC 1 cut(s) 2328
FspAI RTGCGCAY 1 cut(s) 5793
FspI TGCGCA 2 cut(s) 1613, 5793
GlaI GCGC 5 cut(s) 899, 1613, 4141, 5765, 5793
GsuI CTGGAG 4 cut(s) 3042, 4474, 4652, 5595
HaeIII GGCC 7 cut(s) 2169, 2376, 3192, 3966, 4292, 4697, 5120
HapII CCGG 8 cut(s) 1154, 2513, 2991, 3065, 3940, 4946, 5182, 5348
HgaI GACGC 3 cut(s) 3774, 5159, 5700
HhaI GCGC 5 cut(s) 900, 1614, 4142, 5766, 5794
Hin6I GCGC 5 cut(s) 898, 1612, 4140, 5764, 5792
HinP1I GCGC 5 cut(s) 898, 1612, 4140, 5764, 5792
HindIII AAGCTT 6 cut(s) 41, 327, 677, 966, 1886, 3459
HpaII CCGG 8 cut(s) 1154, 2513, 2991, 3065, 3940, 4946, 5182, 5348
HphI GGTGA 7 cut(s) 57, 322, 1925, 2528, 3801, 3869, 5672
Hpy99I CGWCG 2 cut(s) 1410, 5694
HpyCH4IV ACGT 3 cut(s) 4471, 4606, 5539
HpyF10VI GCNNNNNNNGC 6 cut(s) 1434, 2504, 2532, 3717, 5326, 5501
HpySE526I ACGT 3 cut(s) 4471, 4606, 5539
HspAI GCGC 5 cut(s) 898, 1612, 4140, 5764, 5792
Kpn2I TCCGGA 3 cut(s) 1153, 3064, 5347
KpnI GGTACC 1 cut(s) 1461
Ksp22I TGATCA 4 cut(s) 3040, 3898, 4309, 5278
LmnI GCTCC 7 cut(s) 2137, 2323, 2504, 3061, 3174, 3958, 4188
MabI ACCWGGT 1 cut(s) 3833
MaeII ACGT 3 cut(s) 4471, 4606, 5539
MfeI CAATTG 3 cut(s) 1385, 1941, 4345
MhlI GDGCHC 4 cut(s) 2273, 2328, 2361, 3742
MlsI TGGCCA 2 cut(s) 3966, 4697
MluI ACGCGT 1 cut(s) 4096
MluNI TGGCCA 2 cut(s) 3966, 4697
MlyI GAGTC 8 cut(s) 101, 877, 2218, 2680, 4073, 4472, 4615, 5729
MmeI TCCRAC 6 cut(s) 495, 709, 2708, 3754, 3854, 4958
Mox20I TGGCCA 2 cut(s) 3966, 4697
Mph1103I ATGCAT 4 cut(s) 6, 1371, 1829, 4032
MroI TCCGGA 3 cut(s) 1153, 3064, 5347
MscI TGGCCA 2 cut(s) 3966, 4697
MslI CAYNNNNRTG 6 cut(s) 1263, 2931, 2941, 3855, 4011, 4322
Msp20I TGGCCA 2 cut(s) 3966, 4697
MspA1I CMGCKG 4 cut(s) 1535, 2193, 3745, 5754
MspI CCGG 8 cut(s) 1154, 2513, 2991, 3065, 3940, 4946, 5182, 5348
MunI CAATTG 3 cut(s) 1385, 1941, 4345
Mva1269I GAATGC 1 cut(s) 643
MvaI CCWGG 9 cut(s) 831, 864, 1425, 1865, 3032, 3581, 3835, 4289, 4840
MvnI CGCG 2 cut(s) 4098, 5764
MwoI GCNNNNNNNGC 6 cut(s) 1434, 2504, 2532, 3717, 5326, 5501
NciI CCSGG 5 cut(s) 2513, 2514, 2992, 3940, 4946
NcoI CCATGG 2 cut(s) 2404, 5294
NdeI CATATG 3 cut(s) 2690, 2703, 3391
NlaIV GGNNCC 7 cut(s) 691, 861, 1459, 1881, 3176, 4036, 4190
NmuCI GTSAC 8 cut(s) 106, 364, 1592, 2516, 2892, 3635, 4961, 5471
NsbI TGCGCA 2 cut(s) 1613, 5793
NsiI ATGCAT 4 cut(s) 6, 1371, 1829, 4032
NspI RCATGY 7 cut(s) 365, 439, 1239, 2930, 4268, 4761, 5045
NspV TTCGAA 1 cut(s) 3261
PaeI GCATGC 1 cut(s) 4761
PagI TCATGA 3 cut(s) 876, 4006, 4921
PaqCI CACCTGC 2 cut(s) 3845, 3958
PceI AGGCCT 3 cut(s) 2169, 2376, 4292
PciI ACATGT 3 cut(s) 435, 2926, 4264
PcsI WCGNNNNNNNCGW 2 cut(s) 789, 5421
PctI GAATGC 1 cut(s) 643
PflMI CCANNNNNTGG 4 cut(s) 2161, 3419, 3962, 5439
PfoI TCCNGGA 1 cut(s) 2990
PleI GAGTC 8 cut(s) 100, 876, 2217, 2679, 4072, 4471, 4615, 5728
PpsI GAGTC 8 cut(s) 100, 876, 2217, 2679, 4072, 4471, 4615, 5728
Ppu21I YACGTR 1 cut(s) 4607
PpuMI RGGWCCY 1 cut(s) 1879
PscI ACATGT 3 cut(s) 435, 2926, 4264
PshBI ATTAAT 2 cut(s) 1326, 3972
Psp124BI GAGCTC 1 cut(s) 2328
Psp5II RGGWCCY 1 cut(s) 1879
Psp6I CCWGG 9 cut(s) 829, 862, 1423, 1863, 3030, 3579, 3833, 4287, 4838
PspGI CCWGG 9 cut(s) 829, 862, 1423, 1863, 3030, 3579, 3833, 4287, 4838
PspN4I GGNNCC 7 cut(s) 691, 861, 1459, 1881, 3176, 4036, 4190
PspPI GGNCC 5 cut(s) 860, 1879, 3191, 4836, 5226
PspPPI RGGWCCY 1 cut(s) 1879
PstI CTGCAG 2 cut(s) 2544, 3538
PstNI CAGNNNCTG 2 cut(s) 110, 1538
PvuII CAGCTG 4 cut(s) 1535, 2193, 3745, 5754
RseI CAYNNNNRTG 6 cut(s) 1263, 2931, 2941, 3855, 4011, 4322
SacI GAGCTC 1 cut(s) 2328
Sau96I GGNCC 5 cut(s) 860, 1879, 3191, 4836, 5226
ScaI AGTACT 2 cut(s) 921, 1083
SchI GAGTC 8 cut(s) 101, 877, 2218, 2680, 4073, 4472, 4615, 5729
SduI GDGCHC 4 cut(s) 2273, 2328, 2361, 3742
SexAI ACCWGGT 1 cut(s) 3833
SfcI CTRYAG 4 cut(s) 2540, 2642, 3534, 4639
SfuI TTCGAA 1 cut(s) 3261
SinI GGWCC 4 cut(s) 860, 1879, 4836, 5226
SmaI CCCGGG 1 cut(s) 2514
SmiMI CAYNNNNRTG 6 cut(s) 1263, 2931, 2941, 3855, 4011, 4322
SmlI CTYRAG 7 cut(s) 2272, 2378, 3269, 4040, 4733, 4966, 5030
SmoI CTYRAG 7 cut(s) 2272, 2378, 3269, 4040, 4733, 4966, 5030
SnaBI TACGTA 1 cut(s) 4607
SpeI ACTAGT 2 cut(s) 1589, 3352
SphI GCATGC 1 cut(s) 4761
SseBI AGGCCT 3 cut(s) 2169, 2376, 4292
SspI AATATT 4 cut(s) 1221, 1850, 3615, 4765
SstI GAGCTC 1 cut(s) 2328
StuI AGGCCT 3 cut(s) 2169, 2376, 4292
StyI CCWWGG 7 cut(s) 2404, 2479, 3370, 4689, 4974, 5294, 5418
TaiI ACGT 3 cut(s) 4474, 4609, 5542
TatI WGTACW 4 cut(s) 919, 1045, 1081, 4373
TauI GCSGC 5 cut(s) 621, 3711, 4244, 4417, 5764
TseFI GTSAC 8 cut(s) 106, 364, 1592, 2516, 2892, 3635, 4961, 5471
Tsp45I GTSAC 8 cut(s) 106, 364, 1592, 2516, 2892, 3635, 4961, 5471
TspGWI ACGGA 7 cut(s) 1253, 1532, 2410, 4647, 5105, 5246, 5768
TspMI CCCGGG 1 cut(s) 2512
Van91I CCANNNNNTGG 4 cut(s) 2161, 3419, 3962, 5439
VpaK11BI GGWCC 4 cut(s) 860, 1879, 4836, 5226
VspI ATTAAT 2 cut(s) 1326, 3972
XagI CCTNNNNNAGG 2 cut(s) 1422, 3758
XbaI TCTAGA 5 cut(s) 787, 1714, 1810, 1993, 2414
XceI RCATGY 7 cut(s) 365, 439, 1239, 2930, 4268, 4761, 5045
XcmI CCANNNNNNNNNTGG 1 cut(s) 3185
XmaI CCCGGG 1 cut(s) 2512
XmaJI CCTAGG 2 cut(s) 2479, 3370
XmiI GTMKAC 1 cut(s) 5260
ZrmI AGTACT 2 cut(s) 921, 1083
Zsp2I ATGCAT 4 cut(s) 6, 1371, 1829, 4032
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.