Rw2G014630

positive regulation of cell-substrate adhesion

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000307
Physical Location & Seq
Reverse (-)
16463332 .. 16470564
7233 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G014630.1

Sequence Viewer

Length: 6222 bp
ATGTTATCCATTCCTGAAAGAGAGTTAGTTGGAATGGATGCTCGTATATTAGAAATAGAGTCTCGCCTGGATCTAGAGTCAAATGATGTTCTTACCGTAGGGATTTGGGGAATGGGGGGAATCGGTAAGACAACTCTTGCTAAGGAAGTATTCAAAAAGATCCGTAACCAATTTCATCATAGTGGCTTTGTTTCCCAAGTTAGATTGCAATCCGAAGTTGAGTTGCAGCGACGTCTCTGTGAATCCTTTTTGGGGGTTGGCAATATAAATATATCTACTGTTGAAAAAGGGATCAAGTTATTGAAGAAGGCACTATTTAAGAAAAAAGTGCTTATCATTCTAGACGATGTTGATAATTTTAAACAAATAGAATGTCTAGCACCTGGAGGCCCGCTTGGAGAGAATATATGGGGTGGAGGGAGTCGACTGATTATAACAACTAGAGATAGGAGCCCATTGAGATACCTTAATGTACAAGAAAATAAAATATATGAGGTCGAAAAATTGAGAGATGAGGAAGCCTTTCAGCTGTTAAGTCAGAAAGCCTTCAAGAAAGACAATCCACCAGAGGAGTTTGTAGCACTGTCCAAGAGTTTTCTGCAATATGCTAGTGGCCTTCCTTTAGCTCATGAAGTTCTGGGGTCATACTTGTCTAGACTAAAGGTAGATGAATGGTCAGAGATATTGCATAGACTAGATGATGATCCGGATAAAGATATTTTCAGTGTGCTTCAAATAAGTTTTGATGGATTACAGGATACAGATAAGAAAATATTCTTGGACATTGCATGTTTCTTCAATGGCGAGGATCATATCCGTGTAAAGAATATATTGAAAGGTTGTGGCTTTTCTTCCAGAATAGGTATAAGTACCCTCATTGACAAATCTCTGATTAAAATCGAAAGAAATAAACTGTGGATGCATGATTTGCTACGGTGCTTGGGTTGGCATATTGTTCGTGGAGAATCTTCTTTTCCGGGTAAACGTAGCAGGTTGTGGCTTGATGACAATACAGACAAGTACGAAGACAGAATGTCATGGCGTTTTGAGGATGCTTGGAACGTGCTGGTGGACAATATGGGAACAACTGCTGTTGAAGGCTTATTCTTAAGCTTGCCTGAAAAAGAAGAAATGTCATTGGATGATGACCCGTTCTTAACTATGCGCAACTTACGATTGCTGAAGATTTATAATGTGAACTTTCTGGATGTGCATTTCAGATATGTCTCTAAAAATTTACGACTTTTAGAATGGCACGAATGCCCTCTAGAATCTTTGCCATCTGATTTTAAATCGGACAAGCTGGTTGAATTCAAGATGCCTAACAGCCGCATTAAGCAACTATGGAATGAAACGCTCTCTCTTAGATTGCTAATACTAATGGATCTTTCCAACTGCCAATATTTAGCCACGACCCCAGACTTCAGTAAGGTTCCAAATCTTGAGAGATTGATCCTTGAAGGTTGTACTGAGTTATCAGTAGTTCACCCAACAATTGGGGATCTCCAGCAACTGGTTTTTCTGAATCTGAAAGGTTGCGCAAGTCTAGAGAGCCTTCCTCAGTCCATCAGCTTGAGATCTCTTCAAATTTTTATTCTTTCAGGATGTTCAAAACTCGAACACTTTCCCGAGATTGTGGGGAACATGGATGCTTTATCAGAACTTTATTTAGATGGCACGGCTATACGGGTGCTGCCTTTATCAATCCAGAATTTGGAAGGCCTTGTTTTGCTAAATCTTAGCGGCTGCAGGAACCTTCCGAGTCTTCCTAGTGTTCTTTGTAGTCATTTGACATCACTGAAATTTCTCTATCTGTCATTGTGCTCAAGCATGGAAAAACTGCCAGAAAGCATAGGCTACTTGAAACACTTGGAGGAGCTTGATGCTTGTGATACTGCTATAAGAAAAGTTCCTGATTCCATTTCAGGTCTCAAGAATCTAAAACTATTGTGTTTCCATGGATGTTCTGGATTCACAGGGTTAGAGTTGCCAAACAAGTTCTCAGGTTTAAGATCTTTGACAACACTAAACCTAGGTGGATGTAATCTCGCAGAAGGGGCAATCCCCCATGACATTGGTGATTTATTCTCACTGCAAAGTCTGGATTTAAGTGAGAACAACATTTTCACTGTACCTGAAAGCATCTCTCAGCTTTCGGAGCTCACAGAAATTTCTCTGTATAAGTGTAGCAAACTACGGTCGTTGCCAAAAGATCTTCCATCAAGTCTAAGGAATGTAAATGTACGTGATTGTCCCATGCTGACAAACTCTTTATCTAGTTGGAGGAGATATCCGCCGCAGAAGGGTTTGAGTATCATAAACTGTCGAAAACCAGAGGAGGATGACATCTTTCCTGAACTCCAAGAGTTTCGTTTGGGAACTCTGGAGGAACTCGATCTTTCCTACTGCCAACACTTGAAGAAGATCCCTGACTTGAATGGGGTTCCAAATCTGAAGAAATTGATCCTTGAAGGTTGTGAAAAATTATCAGAACTTCACCCAACAATTGGGAGTCTCCAACATCTGATTCTTTTGAATTTGAAAGGATGTGTAGATCTAGAGAGCCTTCCCCGTTTCATCAGCTTGAAGTCTCTAAAAATATTTGTTCTTTCAGGATGTTCAAAGCTGAAAGAGTTTCCAGATATTAAGGGAGATATGGATAGTTTGTCTCAACTGCATTTAGATGGGACGGCGTTAAGGAGACTATCAATACCGATGAAGCATTTTAAATGCCCTATTTCGATAAATCTAAGAGGCTGTAGGAACCTTTTCACTGTTCCAGTTCTACGAAGTCTGAAATCTCTCAATCTGTCGGGCTGCTCACGTGTATCCATATTGCCATCTAACCTGGAAAGCATGAAACATTTGGAGGAGCTTGATGCCTCTGAAACTTCTATAACAGCAGTACCCTCATCCATTTCATATTTGGTGAAGCTTGAAGTGCTATCTTTTTGTGGATGTAGAGGTTTGCAGTTGCCTAAACGGTTCTCGGATTTACTATCTTTGAAATCATTAAATCTACGGAGGTGTGGTCTAGCAGAAGAAGCGCTTGATATCCTGTACTGCTTATCCTCTCTGAAGATTTTGGATTTGGGTGAGAACAGTTTTGTGACTATACCTAATGACATCGATCGCTTATCCTCTTTGCGGCAATTGGATTTGAGTGGAAACAATATAGTGAGCATACCGCATGAAATTGGTTGCTTATACTCGTTGCAGCTTTTAGATTTGAGTGAAAACAACTTTGTGAGTATACCTAAGAGCATCTCTAATCTTTCTGAGCTTACAATACTTCGCCTGTTTAGATGTAGTAAGCTACAGTCCTTGCCAAATAATCTTCCCTTCAATCTAAAACATGTTTATGCACAAGAATGTCCTGTGTTGAAGTATAATGCCGATACTTTGACAATACGGGCATCCGGAGAAGGGGTCTTTTTCATGCATTGCGGAAAATATGAGCAGGATGATGATCAGCCAAGCCATGTCCTAGTTCCAGCTCCCAAAGACCGCATTGAACTACTGTTTCCTGTTTACATTGCGGACCGAGTTTATGGTAAAAAGCCATTTGCAATTTGTTTTCCACACAGTACAAGAATTCCCAATCTGTGGAGTCATTGGAGGAGTGGCCCTTTTGTAACAATCCCACTATCTGATGGTAACAGTGCGTGTATGGGACTTGCTCTGTTTGTTGTTTTTGAAATCCTTGAAGGCAATTTCAATAAGATATGGGAATTGGAAGAGACTATCTGTGAATTTCACACTGATGTTGGTCCTGAAAATGCCCTTGTTTTTCAAAACTTCATAGACTTCAAGGCTGGGTCATATGCACTTTGTTGCTATGAACCGCGAGGGGGGCAATTTAGTGGGATGTTTGATAAACCAATCTCACAACTTCGAGCTTCAGTTTCAATGAAGAGACCAAATTCAAAAGTGAGAGGTTGTGGGATACATCTAATGTCAGAGGAAGACACTGCAGAGTTTGTAAAAAGTTTAGCAAATCAGACTGCCACTCAACACCTCGATTCCAATTTTGATCAACATTGCGAGTACCTATTAAATGAGGAGACAACAACTGGTGACCTGATGGAAGTGGGATCTACTTCAACTTTCAATGAAGATAGCTGCAGCAAAAGTATCTCTAAATTCAACTTGAGAGGAGAGCTGTCGATACTCTATGAGGGATGCAATGGACGTGAGAATAGTTTCCATTTTTGCTTTCCTGCTCCCGTAATTTCAGCCCCGCCCTGGTTCTTCCATCACCATGCAGGGGATGTAACTTTGTGTTACATCTCTAAAAACTTACTTGATGATCAGAGATGGGTGGGATTAGAACTATATGTTCAATTTTCTCGGTGCACATCTACTTCTACATCTGGCAATAGTAGTTTTTTCTTTTATGTTGATTTGTGCTCTCATGATCATGAAAGTATAGTAAGGCACGGATCCCTCAAGATAAACAGTTGTGTTGGGATCTCAGATCAACTTGTTGTATTACATGTACCACGTGTTCATTTTCAACAGCAGTTGAATCAATGTCAGTGTATTAGCGCATTGTTCAGAACCATTGATGCAGAGATGGATGTCCAAGTGTGTGGAAGCCGTTTAGCTTTCGAGCATGATTTGGAAGACTTGATCCATTCATTAACTGCTGCTGTCGGCACGTTGGGGCAACATGTCCTCACTCAACCACGCTCCCCGGCCCAACCTGTTGATCGGCTCAATGCAGAGGAAGCAGAAATGCCCATCAATTGCTGTTCCTGTTTTCAGAGATGCAGAAGTACTGCACTAGTACTACCAGAACAAGCTCCTTCTTCTTCAATTGTGCGGCTGCGAAATTATAGATGCTATCTCCAGCAACAGCAAGGTTTTTGGGAGTCTGACGTTAACCAGTTGGTCCACTTCAGATCAGTAATGTTACTCCACTCCAGAAGCCTTTTGGAAAACAGGGACAGAAACCAAGAAAACATTGCCGAAAAAGATAAATCGTTGGTGTTGGCTCGTCATCATGTACATATAATGGCTGAGACTCAACTTCAGGGAAGCTATAGTTCACCACGGTGGAAGAGATGCCTGAAGTTGTTGCTTAGGCGGTACAAGGTGGCTACTCTCAGTCTCGGTGGACACGAAATTTCAGCTCTCAAGAATTTTGATCCTTCCTCCCCATACAATATTATTTGTTTCTCTGACAACGAAATTCCAGTGTGGTTCAAACATGAGATGTCGTATCAGATGTCTTCTAGGTCTTGGGTGGGAATCAAACTACCTCCAAGATTGCACGAGGATGAGAATTGGAGAGGACTTGCTATATGTGTTACCTTCGAAGTTCATGACCAGCGTCCACCTACCTCCCCGGTCAAACTTCTCTGTCACTTGAGAGCCAAGGACAACTATTGCTTGAATCCTATCCCTATGTGTTCCATCACTGAAGAGAAACTCAAGTCGTTGCATCTTGGTCGATTCATTTGGGTAACCTACATCCCCAGTATTTTTCTAACGGAGTTCAGTGTGATTAGTGATGTAGAGGCCAGAATTTATGTTAGTTGCAGAGGCTTGACAGTGGAGAAGTGTGGTTTACGTCTCTTGTACCGGCAAGAAGAGGGGGAGTTTGAGAACACAATAACCGAATCCTGGACCTCTTTCTTCGATAATCTGTCTTTCATCCGTCAACTTGTAGAAGCAGATGAACTTCCAATGCTTGAAGGGCATAACAAGGTCTTTGATCCGGATTTAATATACAATGCAATCTGCCCTTCTAATGAAATTCTGGAGTGGTTCGGGCATCGCATTGAGGATTCCTCAGATGACACCTGGGGCTGCGAATTCCAGTTACCACCATCTTTGAGCGACACAAATTGGATAGGATTGTCCATCTGTGTATCATATCGTATTAGAAGAGTGGATCGGTACGAACCTAATTCCCACCCCTTTAATTTAACACTGAAAACCAGAAACAATGCTTTGTCGTCTCTCCATCGGTATCAAATTAGCAATGAAGAATATAAGTTCGTAAACCGTTGTCGTATTATGAATCGTAGATTCATTTGGCTCTCCTACATACCACGACGCTGGTTTCTACATCAGCTAAATGATGAGTCTGTCCTCTTTGCTTCACTCCGGGACACCTGGTGGAGCCCGGCCAAGATCTATCATCGATTTGTATATGCGCAGGAAGAGTTCAAGCAGCTCTGTTTCAATCTCGACCGACCTGGTGCCCCACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04064 ko04115 ko04210 map04064 map04115 map04210
Pfam Domains
Protein Families
Death LRR_8 Peptidase_S68 ZU5

Protein Analysis

2073

Amino Acids

236.03

Weight (kDa)

6.09

Isoelectric Point (pI)

45.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 19 - 186 2.3e-21 NB-ARC domain
NACHT PF05729 32 - 180 5.1e-06 NACHT domain
WHD_ROQ1 PF23282 254 - 323 2.4e-18 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 472 - 580 4.1e-06 Leucine-rich repeat region
LRR_13 PF23286 525 - 593 5.4e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 567 - 678 5.6e-13 Leucine-rich repeat region
LRR_14 PF23598 781 - 894 1e-05 Leucine-rich repeat region
LRR_14 PF23598 921 - 1031 2.7e-09 Leucine-rich repeat region
LRR_8 PF13855 1001 - 1059 2.2e-08 Leucine rich repeat
LRR_14 PF23598 1020 - 1108 1.8e-11 Leucine-rich repeat region
C-JID PF20160 1728 - 1865 8.4e-08 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000307)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g16600 FvH4_1g16610 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16621 FvH4_1g16630 FvH4_1g16640 FvH4_1g16640 FvH4_1g16650 FvH4_1g16650 FvH4_3g27631 FvH4_4g11392 FvH4_4g11542
prunus_persica Prupe.4G224500_v2.0.a1 Prupe.4G226900_v2.0.a1 Prupe.4G227000_v2.0.a1
pyrus_communis pycom11g18180 pycom11g18210 pycom11g18230 pycom11g18270 pycom11g18350 pycom11g18360 pycom11g18370
rosa_chinensis RchiOBHm_Chr2g0106511 RchiOBHm_Chr2g0106541 RchiOBHm_Chr2g0106551 RchiOBHm_Chr2g0106571 RchiOBHm_Chr2g0124621 RchiOBHm_Chr6g0251591 RchiOBHm_Chr6g0251631 RchiOBHm_Chr6g0251641
rosa_laevigata RLG00000015443 RLG00000015444 RLG00000015458 RLG00000017510 RLG00000017511 RLG00000017512 RLG00000017515 RLG00000017516 RLG00000017517 RLG00000018809 RLG00000020934 RLG00000020935 RLG00000034092
rosa_multiflora Rmu_co8285777.1_g000001 Rmu_sc0000762.1_g000001 Rmu_sc0001872.1_g000006 Rmu_sc0005255.1_g000019 Rmu_sc0005597.1_g000006 Rmu_sc0012104.1_g000001 Rmu_sc0017105.1_g000011 Rmu_sc0022904.1_g000001 Rmu_ssc0000135.1_g000049 Rmu_ssc0000213.1_g000047
rosa_roxburghii Rroxscaffold_2G00092830 Rroxscaffold_2G00119790 Rroxscaffold_2G00137010 Rroxscaffold_2G00137030 Rroxscaffold_2G00137070 Rroxscaffold_7G00216560
rosa_rugosa Rorug02G0136100 Rorug02G0136200 Rorug02G0136300 Rorug02G0136400 Rorug02G0251800 Rorug02G0251800 Rorug02G0455500 Rorug02G0455600 Rorug02G0455700 Rorug05G0195300
rosa_samantha Rh2AG187400 Rh2AG187500 Rh2AG187600 Rh2AG310500 Rh2BG198200 Rh2BG198300 Rh2BG198400 Rh2BG198600 Rh2BG318900 Rh2BG534300 Rh2CG192400 Rh2CG192500 Rh2CG192600 Rh2CG297500 Rh2DG193500 Rh2DG193600 Rh2DG193800 Rh2DG334200 Rh2DG543100 Rh5AG280400 Rh5BG285800 Rh5BG285900 Rh5CG317900 Rh5CG318000 Rh5DG294600 Rh5DG294700 Rh6AG012200 Rh6AG012700 Rh6BG011200 Rh6BG011700
rosa_wichuraiana Rw0G009380 Rw2G014610 Rw2G014630 Rw2G014640 Rw2G014680 Rw2G025120 Rw2G043000 Rw5G026490 Rw6G001370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 434, 1191
AatII GACGTC 1 cut(s) 235
Acc16I TGCGCA 3 cut(s) 1166, 1540, 6165
Acc36I ACCTGC 1 cut(s) 981
AccB1I GGYRCC 1 cut(s) 6209
AccB7I CCANNNNNTGG 5 cut(s) 1496, 1513, 1714, 2507, 3606
AccI GTMKAC 2 cut(s) 424, 3253
AccII CGCG 1 cut(s) 3847
AccIII TCCGGA 3 cut(s) 706, 3419, 5722
AcoI YGGCCR 1 cut(s) 6135
AcuI CTGAAG 9 cut(s) 1202, 1408, 2474, 3098, 3885, 4885, 5018, 5093, 5445
AcvI CACGTG 2 cut(s) 2825, 4505
AcyI GRCGYC 1 cut(s) 232
AdeI CACNNNGTG 1 cut(s) 6126
AfeI AGCGCT 1 cut(s) 3048
AflII CTTAAG 1 cut(s) 1108
AflIII ACRYGT 5 cut(s) 2824, 3355, 4495, 4504, 4672
AhdI GACNNNNNGTC 1 cut(s) 1033
AhlI ACTAGT 1 cut(s) 4786
AjiI CACGTC 1 cut(s) 4193
AjnI CCWGG 8 cut(s) 66, 382, 2847, 4244, 5627, 5807, 6122, 6205
AjuI GAANNNNNNNTTGG 4 cut(s) 761, 793, 2511, 2543
AleI CACNNNNGTG 1 cut(s) 5056
Alw21I GWGCWC 4 cut(s) 1826, 2163, 4358, 4412
Alw44I GTGCAC 1 cut(s) 4354
AlwNI CAGNNNCTG 1 cut(s) 1513
Ama87I CYCGRG 1 cut(s) 1628
Aor13HI TCCGGA 3 cut(s) 706, 3419, 5722
Aor51HI AGCGCT 1 cut(s) 3048
AoxI GGCC 7 cut(s) 388, 613, 1720, 3625, 4698, 5523, 6135
ApaLI GTGCAC 1 cut(s) 4354
ArsI GACNNNNNNTTYG 2 cut(s) 358, 390
Asp700I GAANNNNTTC 6 cut(s) 522, 545, 773, 1623, 2768, 4202
AspA2I CCTAGG 1 cut(s) 2032
AspLEI GCGC 5 cut(s) 1167, 1541, 3049, 4550, 6166
AspS9I GGNCC 7 cut(s) 389, 3541, 3626, 3770, 4699, 4894, 5631
AsuC2I CCSGG 5 cut(s) 978, 4697, 5351, 6116, 6134
AsuHPI GGTGA 8 cut(s) 1478, 2090, 2489, 2941, 3107, 4088, 4250, 5043
AsuII TTCGAA 1 cut(s) 5319
AvaI CYCGRG 1 cut(s) 1628
AvaII GGWCC 4 cut(s) 3541, 3770, 4894, 5631
AvrII CCTAGG 1 cut(s) 2032
BaeGI GKGCMC 2 cut(s) 4358, 6214
BaeI ACNNNNGTAYC 2 cut(s) 2889, 2922
BamHI GGATCC 1 cut(s) 4442
BanI GGYRCC 1 cut(s) 6209
BanII GRGCYC 3 cut(s) 455, 2163, 6134
BauI CACGAG 1 cut(s) 5276
BbrPI CACGTG 2 cut(s) 2825, 4505
BbsI GAAGAC 5 cut(s) 1032, 1757, 3972, 4632, 5226
Bbv12I GWGCWC 4 cut(s) 1826, 2163, 4358, 4412
BceAI ACGGC 3 cut(s) 1695, 2706, 4584
BcgI CGANNNNNNTGC 4 cut(s) 938, 972, 5435, 5469
BciT130I CCWGG 8 cut(s) 68, 384, 2849, 4246, 5629, 5809, 6124, 6207
BciVI GTATCC 3 cut(s) 751, 2839, 3939
BclI TGATCA 4 cut(s) 3469, 4033, 4309, 4417
BcnI CCSGG 5 cut(s) 978, 4697, 5351, 6116, 6134
BcuI ACTAGT 1 cut(s) 4786
BfmI CTRYAG 6 cut(s) 1747, 2758, 3317, 3972, 4123, 5044
BfoI RGCGCY 1 cut(s) 3050
BfrI CTTAAG 1 cut(s) 1108
BfuAI ACCTGC 1 cut(s) 981
BfuI GTATCC 3 cut(s) 751, 2839, 3939
BglII AGATCT 5 cut(s) 1577, 2012, 2212, 2554, 6141
BlnI CCTAGG 1 cut(s) 2032
BmcAI AGTACT 2 cut(s) 4780, 4791
Bme18I GGWCC 4 cut(s) 3541, 3770, 4894, 5631
BmeRI GACNNNNNGTC 1 cut(s) 1033
BmeT110I CYCGRG 1 cut(s) 1628
BmgBI CACGTC 1 cut(s) 4193
BmgT120I GGNCC 7 cut(s) 389, 3541, 3626, 3770, 4699, 4894, 5631
BmiI GGNNCC 8 cut(s) 452, 1434, 1754, 2445, 2765, 4444, 6131, 6211
BmrI ACTGGG 1 cut(s) 5475
BmuI ACTGGG 1 cut(s) 5475
BoxI GACNNNNGTC 1 cut(s) 5334
BpiI GAAGAC 5 cut(s) 1032, 1757, 3972, 4632, 5226
BplI GAGNNNNNCTC 4 cut(s) 1543, 1575, 5780, 5812
BpmI CTGGAG 6 cut(s) 405, 1490, 2405, 4835, 4909, 5786
Bpu10I CCTNAGC 2 cut(s) 141, 5084
Bpu14I TTCGAA 1 cut(s) 5319
BpuEI CTTGAG 9 cut(s) 1463, 1594, 1810, 1916, 4171, 4433, 5123, 5392, 5420
BpuMI CCSGG 5 cut(s) 978, 4697, 5351, 6116, 6134
Bsa29I ATCGAT 2 cut(s) 3129, 6151
BsaAI YACGTR 3 cut(s) 2246, 2825, 4505
BsaBI GATNNNNATC 4 cut(s) 208, 702, 896, 3468
BsaHI GRCGYC 1 cut(s) 232
BsaI GGTCTC 2 cut(s) 1934, 3910
BsaJI CCNNGG 8 cut(s) 1957, 2032, 4244, 4695, 5054, 5349, 5379, 5808
BsaWI WCCGGW 3 cut(s) 706, 3419, 5722
BsaXI ACNNNNNCTCC 4 cut(s) 3016, 3046, 3414, 3444
Bse118I RCCGGY 1 cut(s) 5586
Bse1I ACTGG 7 cut(s) 1518, 2780, 4078, 4888, 5198, 5481, 5824
Bse3DI GCAATG 7 cut(s) 783, 3442, 3534, 4039, 4192, 4965, 5995
Bse8I GATNNNNATC 4 cut(s) 208, 702, 896, 3468
BseAI TCCGGA 3 cut(s) 706, 3419, 5722
BseBI CCWGG 8 cut(s) 68, 384, 2849, 4246, 5629, 5809, 6124, 6207
BseCI ATCGAT 2 cut(s) 3129, 6151
BseDI CCNNGG 8 cut(s) 1957, 2032, 4244, 4695, 5054, 5349, 5379, 5808
BseJI GATNNNNATC 4 cut(s) 208, 702, 896, 3468
BseMI GCAATG 7 cut(s) 783, 3442, 3534, 4039, 4192, 4965, 5995
BseMII CTCAG 9 cut(s) 1461, 1574, 2016, 2162, 3270, 4488, 5013, 5122, 5811
BseNI ACTGG 7 cut(s) 1518, 2780, 4078, 4888, 5198, 5481, 5824
BseRI GAGGAG 8 cut(s) 584, 1889, 2299, 2351, 2885, 3634, 4076, 4170
BseSI GKGCMC 2 cut(s) 4358, 6214
BseYI CCCAGC 1 cut(s) 3815
BsgI GTGCAG 1 cut(s) 4767
Bsh1236I CGCG 1 cut(s) 3847
Bsh1285I CGRYCG 3 cut(s) 2201, 3133, 6202
BshFI GGCC 7 cut(s) 390, 615, 1722, 3627, 4700, 5525, 6137
BshNI GGYRCC 1 cut(s) 6209
BshVI ATCGAT 2 cut(s) 3129, 6151
BsiEI CGRYCG 3 cut(s) 2201, 3133, 6202
BsiHKAI GWGCWC 4 cut(s) 1826, 2163, 4358, 4412
BsiHKCI CYCGRG 1 cut(s) 1628
BsiSI CCGG 9 cut(s) 707, 977, 3420, 4697, 5351, 5587, 5723, 6115, 6134
BslFI GGGAC 5 cut(s) 2238, 2701, 3687, 4961, 6131
BsmBI CGTCTC 3 cut(s) 239, 5582, 5970
BsmFI GGGAC 5 cut(s) 2238, 2701, 3687, 4961, 6131
BsmI GAATGC 1 cut(s) 1265
BsnI GGCC 7 cut(s) 390, 615, 1722, 3627, 4700, 5525, 6137
Bso31I GGTCTC 2 cut(s) 1934, 3910
BsoBI CYCGRG 1 cut(s) 1628
Bsp119I TTCGAA 1 cut(s) 5319
Bsp1286I GDGCHC 7 cut(s) 455, 1826, 2163, 4358, 4412, 6134, 6214
Bsp13I TCCGGA 3 cut(s) 706, 3419, 5722
Bsp1407I TGTACA 2 cut(s) 472, 5008
Bsp19I CCATGG 1 cut(s) 1957
BspANI GGCC 7 cut(s) 390, 615, 1722, 3627, 4700, 5525, 6137
BspCNI CTCAG 9 cut(s) 1462, 1573, 2015, 2161, 3271, 4487, 5014, 5121, 5810
BspDI ATCGAT 2 cut(s) 3129, 6151
BspEI TCCGGA 3 cut(s) 706, 3419, 5722
BspFNI CGCG 1 cut(s) 3847
BspHI TCATGA 4 cut(s) 628, 4414, 4420, 5326
BspLI GGNNCC 8 cut(s) 452, 1434, 1754, 2445, 2765, 4444, 6131, 6211
BspMAI CTGCAG 3 cut(s) 1751, 3976, 4127
BspMI ACCTGC 1 cut(s) 981
BspT104I TTCGAA 1 cut(s) 5319
BspT107I GGYRCC 1 cut(s) 6209
BspTI CTTAAG 1 cut(s) 1108
BspTNI GGTCTC 2 cut(s) 1934, 3910
BsrDI GCAATG 7 cut(s) 783, 3442, 3534, 4039, 4192, 4965, 5995
BsrFI RCCGGY 1 cut(s) 5586
BsrGI TGTACA 2 cut(s) 472, 5008
BsrI ACTGG 7 cut(s) 1518, 2780, 4078, 4888, 5198, 5481, 5824
BssAI RCCGGY 1 cut(s) 5586
BssECI CCNNGG 8 cut(s) 1957, 2032, 4244, 4695, 5054, 5349, 5379, 5808
BssNAI GTATAC 1 cut(s) 3254
BssNI GRCGYC 1 cut(s) 232
BssSI CACGAG 1 cut(s) 5276
BssT1I CCWWGG 3 cut(s) 1957, 2032, 5379
Bst1107I GTATAC 1 cut(s) 3254
Bst2BI CACGAG 1 cut(s) 5276
Bst2UI CCWGG 8 cut(s) 68, 384, 2849, 4246, 5629, 5809, 6124, 6207
Bst6I CTCTTC 8 cut(s) 1587, 3732, 3908, 5057, 5421, 5589, 5887, 6165
BstACI GRCGYC 1 cut(s) 232
BstAFI CTTAAG 1 cut(s) 1108
BstAPI GCANNNNNTGC 1 cut(s) 928
BstAUI TGTACA 2 cut(s) 472, 5008
BstBAI YACGTR 3 cut(s) 2246, 2825, 4505
BstBI TTCGAA 1 cut(s) 5319
BstC8I GCNNGC 2 cut(s) 392, 1115
BstDSI CCRYGG 2 cut(s) 1957, 5054
BstEII GGTNACC 2 cut(s) 4076, 5467
BstFNI CGCG 1 cut(s) 3847
BstH2I RGCGCY 1 cut(s) 3050
BstHHI GCGC 5 cut(s) 1167, 1541, 3049, 4550, 6166
BstMCI CGRYCG 3 cut(s) 2201, 3133, 6202
BstMWI GCNNNNNNNGC 8 cut(s) 928, 2057, 2158, 2941, 3044, 3853, 4730, 5701
BstNI CCWGG 8 cut(s) 68, 384, 2849, 4246, 5629, 5809, 6124, 6207
BstNSI RCATGY 4 cut(s) 792, 3359, 4499, 4676
BstPAI GACNNNNGTC 1 cut(s) 5334
BstPI GGTNACC 2 cut(s) 4076, 5467
BstSFI CTRYAG 6 cut(s) 1747, 2758, 3317, 3972, 4123, 5044
BstSLI GKGCMC 2 cut(s) 4358, 6214
BstUI CGCG 1 cut(s) 3847
BstV2I GAAGAC 5 cut(s) 1032, 1757, 3972, 4632, 5226
BstXI CCANNNNNNTGG 3 cut(s) 2075, 4592, 6066
BstZ17I GTATAC 1 cut(s) 3254
Bsu15I ATCGAT 2 cut(s) 3129, 6151
BsuI GTATCC 3 cut(s) 751, 2839, 3939
BsuRI GGCC 7 cut(s) 390, 615, 1722, 3627, 4700, 5525, 6137
BsuTUI ATCGAT 2 cut(s) 3129, 6151
BtgI CCRYGG 2 cut(s) 1957, 5054
BtgZI GCGATG 1 cut(s) 5765
BtrI CACGTC 1 cut(s) 4193
BtsI GCAGTG 2 cut(s) 2090, 3969
BveI ACCTGC 1 cut(s) 981
Cac8I GCNNGC 2 cut(s) 392, 1115
CaiI CAGNNNCTG 1 cut(s) 1513
CciI TCATGA 4 cut(s) 628, 4414, 4420, 5326
CfoI GCGC 5 cut(s) 1167, 1541, 3049, 4550, 6166
Cfr10I RCCGGY 1 cut(s) 5586
Cfr13I GGNCC 7 cut(s) 389, 3541, 3626, 3770, 4699, 4894, 5631
ClaI ATCGAT 2 cut(s) 3129, 6151
CpoI CGGWCCG 1 cut(s) 3541
CseI GACGC 2 cut(s) 5324, 6072
CsiI ACCWGGT 2 cut(s) 6122, 6205
CspCI CAANNNNNGTGG 2 cut(s) 5820, 5855
CspI CGGWCCG 1 cut(s) 3541
DraI TTTAAA 3 cut(s) 361, 1291, 2728
DraIII CACNNNGTG 1 cut(s) 6126
DriI GACNNNNNGTC 1 cut(s) 1033
EaeI YGGCCR 1 cut(s) 6135
Eam1104I CTCTTC 8 cut(s) 1587, 3732, 3908, 5057, 5421, 5589, 5887, 6165
Eam1105I GACNNNNNGTC 1 cut(s) 1033
EarI CTCTTC 8 cut(s) 1587, 3732, 3908, 5057, 5421, 5589, 5887, 6165
EciI GGCGGA 1 cut(s) 2283
Ecl136II GAGCTC 1 cut(s) 2161
Eco130I CCWWGG 3 cut(s) 1957, 2032, 5379
Eco147I AGGCCT 1 cut(s) 1722
Eco24I GRGCYC 3 cut(s) 455, 2163, 6134
Eco31I GGTCTC 2 cut(s) 1934, 3910
Eco32I GATATC 2 cut(s) 2291, 3055
Eco47I GGWCC 4 cut(s) 3541, 3770, 4894, 5631
Eco47III AGCGCT 1 cut(s) 3048
Eco53kI GAGCTC 1 cut(s) 2161
Eco57I CTGAAG 9 cut(s) 1202, 1408, 2474, 3098, 3885, 4885, 5018, 5093, 5445
Eco72I CACGTG 2 cut(s) 2825, 4505
Eco88I CYCGRG 1 cut(s) 1628
Eco91I GGTNACC 2 cut(s) 4076, 5467
EcoICRI GAGCTC 1 cut(s) 2161
EcoO65I GGTNACC 2 cut(s) 4076, 5467
EcoRI GAATTC 3 cut(s) 1310, 3594, 5819
EcoRII CCWGG 8 cut(s) 66, 382, 2847, 4244, 5627, 5807, 6122, 6205
EcoRV GATATC 2 cut(s) 2291, 3055
EcoT14I CCWWGG 3 cut(s) 1957, 2032, 5379
EcoT22I ATGCAT 2 cut(s) 924, 3444
EcoT38I GRGCYC 3 cut(s) 455, 2163, 6134
ErhI CCWWGG 3 cut(s) 1957, 2032, 5379
Esp3I CGTCTC 3 cut(s) 239, 5582, 5970
FalI AAGNNNNNCTT 2 cut(s) 3033, 3065
FaqI GGGAC 5 cut(s) 2238, 2701, 3687, 4961, 6131
FauI CCCGC 2 cut(s) 399, 4248
FauNDI CATATG 1 cut(s) 3823
FbaI TGATCA 4 cut(s) 3469, 4033, 4309, 4417
FblI GTMKAC 2 cut(s) 424, 3253
FriOI GRGCYC 3 cut(s) 455, 2163, 6134
FspI TGCGCA 3 cut(s) 1166, 1540, 6165
GlaI GCGC 5 cut(s) 1166, 1540, 3048, 4549, 6165
GsaI CCCAGC 1 cut(s) 3819
GsuI CTGGAG 6 cut(s) 405, 1490, 2405, 4835, 4909, 5786
HaeII RGCGCY 1 cut(s) 3050
HaeIII GGCC 7 cut(s) 390, 615, 1722, 3627, 4700, 5525, 6137
HapII CCGG 9 cut(s) 707, 977, 3420, 4697, 5351, 5587, 5723, 6115, 6134
HgaI GACGC 2 cut(s) 5324, 6072
HhaI GCGC 5 cut(s) 1167, 1541, 3049, 4550, 6166
Hin1I GRCGYC 1 cut(s) 232
Hin6I GCGC 5 cut(s) 1165, 1539, 3047, 4548, 6164
HinP1I GCGC 5 cut(s) 1165, 1539, 3047, 4548, 6164
HincII GTYRAC 3 cut(s) 425, 4885, 5666
HindII GTYRAC 3 cut(s) 425, 4885, 5666
HindIII AAGCTT 2 cut(s) 1111, 2933
HpaI GTTAAC 1 cut(s) 4885
HpaII CCGG 9 cut(s) 707, 977, 3420, 4697, 5351, 5587, 5723, 6115, 6134
HphI GGTGA 8 cut(s) 1478, 2090, 2489, 2941, 3107, 4088, 4250, 5043
Hpy99I CGWCG 2 cut(s) 234, 6066
HpyF10VI GCNNNNNNNGC 8 cut(s) 928, 2057, 2158, 2941, 3044, 3853, 4730, 5701
Hsp92I GRCGYC 1 cut(s) 232
HspAI GCGC 5 cut(s) 1165, 1539, 3047, 4548, 6164
Kpn2I TCCGGA 3 cut(s) 706, 3419, 5722
Ksp22I TGATCA 4 cut(s) 3469, 4033, 4309, 4417
KspAI GTTAAC 1 cut(s) 4885
LmnI GCTCC 9 cut(s) 450, 1876, 2158, 2872, 3502, 4228, 4697, 4810, 6129
MabI ACCWGGT 2 cut(s) 6122, 6205
MfeI CAATTG 5 cut(s) 1494, 2505, 3152, 4747, 4818
MhlI GDGCHC 7 cut(s) 455, 1826, 2163, 4358, 4412, 6134, 6214
MlyI GAGTC 9 cut(s) 68, 86, 430, 1771, 2521, 3619, 4883, 5020, 6101
MmeI TCCRAC 4 cut(s) 10, 1416, 2261, 2542
Mph1103I ATGCAT 2 cut(s) 924, 3444
MroI TCCGGA 3 cut(s) 706, 3419, 5722
MroXI GAANNNNTTC 6 cut(s) 522, 545, 773, 1623, 2768, 4202
MspA1I CMGCKG 1 cut(s) 529
MspCI CTTAAG 1 cut(s) 1108
MspI CCGG 9 cut(s) 707, 977, 3420, 4697, 5351, 5587, 5723, 6115, 6134
MunI CAATTG 5 cut(s) 1494, 2505, 3152, 4747, 4818
Mva1269I GAATGC 1 cut(s) 1265
MvaI CCWGG 8 cut(s) 68, 384, 2849, 4246, 5629, 5809, 6124, 6207
MvnI CGCG 1 cut(s) 3847
MwoI GCNNNNNNNGC 8 cut(s) 928, 2057, 2158, 2941, 3044, 3853, 4730, 5701
NciI CCSGG 5 cut(s) 978, 4697, 5351, 6116, 6134
NcoI CCATGG 1 cut(s) 1957
NdeI CATATG 1 cut(s) 3823
NlaIV GGNNCC 8 cut(s) 452, 1434, 1754, 2445, 2765, 4444, 6131, 6211
NmuCI GTSAC 3 cut(s) 3109, 4076, 5366
NsbI TGCGCA 3 cut(s) 1166, 1540, 6165
NsiI ATGCAT 2 cut(s) 924, 3444
NspI RCATGY 4 cut(s) 792, 3359, 4499, 4676
NspV TTCGAA 1 cut(s) 5319
OliI CACNNNNGTG 1 cut(s) 5056
PagI TCATGA 4 cut(s) 628, 4414, 4420, 5326
PceI AGGCCT 1 cut(s) 1722
PciI ACATGT 3 cut(s) 3355, 4495, 4672
PcsI WCGNNNNNNNCGW 1 cut(s) 5121
PctI GAATGC 1 cut(s) 1265
PdmI GAANNNNTTC 6 cut(s) 522, 545, 773, 1623, 2768, 4202
PflMI CCANNNNNTGG 5 cut(s) 1496, 1513, 1714, 2507, 3606
PfoI TCCNGGA 2 cut(s) 5627, 6114
Ple19I CGATCG 1 cut(s) 3133
PleI GAGTC 9 cut(s) 67, 85, 429, 1770, 2520, 3618, 4882, 5020, 6100
PmaCI CACGTG 2 cut(s) 2825, 4505
PmlI CACGTG 2 cut(s) 2825, 4505
PpsI GAGTC 9 cut(s) 67, 85, 429, 1770, 2520, 3618, 4882, 5020, 6100
Ppu21I YACGTR 3 cut(s) 2246, 2825, 4505
PscI ACATGT 3 cut(s) 3355, 4495, 4672
PshAI GACNNNNGTC 1 cut(s) 5334
PsiI TTATAA 2 cut(s) 434, 1191
Psp124BI GAGCTC 1 cut(s) 2163
Psp6I CCWGG 8 cut(s) 66, 382, 2847, 4244, 5627, 5807, 6122, 6205
PspCI CACGTG 2 cut(s) 2825, 4505
PspEI GGTNACC 2 cut(s) 4076, 5467
PspFI CCCAGC 1 cut(s) 3815
PspGI CCWGG 8 cut(s) 66, 382, 2847, 4244, 5627, 5807, 6122, 6205
PspN4I GGNNCC 8 cut(s) 452, 1434, 1754, 2445, 2765, 4444, 6131, 6211
PspPI GGNCC 7 cut(s) 389, 3541, 3626, 3770, 4699, 4894, 5631
PsrI GAACNNNNNNTAC 2 cut(s) 4491, 4523
PstI CTGCAG 3 cut(s) 1751, 3976, 4127
PstNI CAGNNNCTG 1 cut(s) 1513
PvuI CGATCG 1 cut(s) 3133
PvuII CAGCTG 1 cut(s) 529
Rsr2I CGGWCCG 1 cut(s) 3541
RsrII CGGWCCG 1 cut(s) 3541
SacI GAGCTC 1 cut(s) 2163
SalI GTCGAC 1 cut(s) 423
Sau96I GGNCC 7 cut(s) 389, 3541, 3626, 3770, 4699, 4894, 5631
ScaI AGTACT 2 cut(s) 4780, 4791
SchI GAGTC 9 cut(s) 68, 86, 430, 1771, 2521, 3619, 4883, 5020, 6101
SduI GDGCHC 7 cut(s) 455, 1826, 2163, 4358, 4412, 6134, 6214
SexAI ACCWGGT 2 cut(s) 6122, 6205
SfcI CTRYAG 6 cut(s) 1747, 2758, 3317, 3972, 4123, 5044
SfuI TTCGAA 1 cut(s) 5319
SinI GGWCC 4 cut(s) 3541, 3770, 4894, 5631
SpeI ACTAGT 1 cut(s) 4786
SseBI AGGCCT 1 cut(s) 1722
SspI AATATT 4 cut(s) 774, 1403, 2601, 5170
SstI GAGCTC 1 cut(s) 2163
StuI AGGCCT 1 cut(s) 1722
StyI CCWWGG 3 cut(s) 1957, 2032, 5379
TaqII GACCGA 2 cut(s) 3558, 6216
TatI WGTACW 7 cut(s) 472, 1466, 3060, 3587, 4778, 4789, 5008
TauI GCSGC 5 cut(s) 1332, 1746, 2299, 3151, 4828
TseFI GTSAC 3 cut(s) 3109, 4076, 5366
Tsp45I GTSAC 3 cut(s) 3109, 4076, 5366
TspGWI ACGGA 6 cut(s) 152, 806, 3037, 4455, 5510, 5651
Van91I CCANNNNNTGG 5 cut(s) 1496, 1513, 1714, 2507, 3606
Vha464I CTTAAG 1 cut(s) 1108
VneI GTGCAC 1 cut(s) 4354
VpaK11BI GGWCC 4 cut(s) 3541, 3770, 4894, 5631
XbaI TCTAGA 6 cut(s) 73, 340, 653, 1267, 1546, 2557
XceI RCATGY 4 cut(s) 792, 3359, 4499, 4676
XcmI CCANNNNNNNNNTGG 3 cut(s) 1964, 2923, 4933
XmaJI CCTAGG 1 cut(s) 2032
XmiI GTMKAC 2 cut(s) 424, 3253
XmnI GAANNNNTTC 6 cut(s) 522, 545, 773, 1623, 2768, 4202
ZraI GACGTC 1 cut(s) 233
ZrmI AGTACT 2 cut(s) 4780, 4791
Zsp2I ATGCAT 2 cut(s) 924, 3444
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.