RLG00000020935

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000307
Physical Location & Seq
Reverse (-)
70270776 .. 70274760
3985 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020935

Sequence Viewer

Length: 2100 bp
ATGAAGATCTTCACATGTTGTGACTTTCATCCTGAAATCGGCATTGTTGACCTTATTGATAAATCTTTGATTAAAATTGAAGGGAAAAAACTGTGGATGCATGATTTACTACAACAAATGGGTTGGCAGACTGTTCGTGAACAATATCCTAAAGAGCCGGGTAAACGTAGTAGGTTGTGGCTTAATGAGAATGCTTGTGAGTACGAACGCTCTAGGTCGTGGTTTTATGAGGAACAGTCAAGCAAACGCAGAAGGTTGTGGCTTTACAAGGATGCTCGTGCTGTACTCATGCATAATCTGCTTTCTGCGGAAATGCTGATACTTATGGATCTTTCAAACTGCCAATACTTGACTAGGACCCCGGATTTCAGTACAGTTCCAAAGCTTGAAAGGTTGGTCCTCGAAGGTTGTAAGAGATTATCAGAGGTTCACCCAACAATTGGAGGTCTCCAACATCTGGTCCTATTAAATTTGAAAGGTTGTGAAAGTTTAGAGAGCCTTCCTGACTCCATCAGCTTGGAGTCTCTTAAGACCTTAGTCCTTTCAGGATGTTCGAAAATCAAAAGGTTTCCAGAGATTATGGGAAACATGGAAAATATGTCGGAACTTTATCTAGATGGGACAGCTATACGGGAGCTGCCAATATCGTTGAAGCACTTAAGTGGCCTTAGTTTGTTAAATCTAAGAGGCTGCAAGAACCTCTCAAGTCTTCCAAGCTTCATTTGTAGTTTGACATCTCTGATATCTCTCAATCTATCAGGCTGTTCACTTATTGATCAATTGCCAGAAAACCTAGGGAGCTTGGAACATTTGCAAGAGCTTGATGCCTGTGAAACTTCCATAACAAAAGTTCCTTCGTCTATTTTAATGCTTCTGTTGAAGAACCTTAAACGATTATGTTTCCGTCGATGTAACGATTTATGGTTGCCAGCTCAGGACCCAATCTCGGTAGGTTTCTGGTCGTTGACATCAAAATGTATGAACAGTAGCAACAGTCCCATTCAACCACCATTGGTTATTGAAAGGCTCAAATCTTCGGAGATTGTGGAAAACATGAAGGGTTTAGAAAAGGTGAACTACACGTCCTCAGGTGCCAAGATGGATTTTTCCTCAAGTGCGGAGATGGATTCATCCTCAAGTGCCAAGATGGAAGTAACTGGAATGGATTCTCGTGTAGAAAAAATGTGGATTTTGGACATGGGACACATAGGAAAGACAACTATTTCACAAGAAGTTTTTGAAAAGATCTGTGACCTATTTGAAGATAGCTGTTTTGTTGGTCCTATTAGAGAGGAATCTGGAAAGCATGGTCTAGTAGTTAGATTGCAACGTGATCTTTTCATATTCAAAATGATGGCCAAGGTTCCTTCGAAGCATTTATGCTTATCCCATTTGACATCATTAAGTGTATCTGGTAGGCATATAGATGATATCCCTGATGATACTGGCCTCATTTTATACTCACTTCAGCGTTTAGATTTGAGTGGAAATATTTATTTAAATTCAGTTAATGATATCGAAGATCATTTAAATATCATTCGTCTTAAATACATTGAGGATCGAATCTATCACGAACAGCCATTTGAAATCCATCTTCATCGCACTAGAATTCCAGACTGGTGTGGTTATTCTAGGAGAGGATCTTCTGTAACAATCCCACTATCTGATCCAGAAGATGGTACTAGTACGTGGATGGGATTTGCCCTTTTTGTTGTTTTCGAAATCTTTGCCAATAGAAACTTTGCTAAGAGTTGGGAATTTAAAGATACTTGTTGTCATTTTAGCACCTACGAAGATCCTCTTGAAAATCCCCTAGTTTTTCGAAACTTCAGTAACCACGGGGTTGGGCCATTTGTGCTCTGTTGCTACGTGCCACAACGACTGTTCTCAAGGAAGTTGAATAGAGCAAGTCGTCTACTTACAGCTTCAATTTCAACGGAGAGACCGGATTTGGAAGTGAAAGAGTGTGGAATACAACTAATATCCCAGAAAGGTGCTGCATTGTCTGTTCAAGATTTAACCCAGACAGCTACTTATCAACTTCATCAGCTGGATCCAAATTTTGTTCTTCCTCCACGTGTCGTAACGGAGGAGTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

700

Amino Acids

79.63

Weight (kDa)

6.21

Isoelectric Point (pI)

53.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_ROQ1 PF23282 4 - 48 1e-08 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 95 - 204 3.7e-06 Leucine-rich repeat region
LRR_13 PF23286 173 - 258 2.4e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 250 - 306 2.2e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000307)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g16600 FvH4_1g16610 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16621 FvH4_1g16630 FvH4_1g16640 FvH4_1g16640 FvH4_1g16650 FvH4_1g16650 FvH4_3g27631 FvH4_4g11392 FvH4_4g11542
prunus_persica Prupe.4G224500_v2.0.a1 Prupe.4G226900_v2.0.a1 Prupe.4G227000_v2.0.a1
pyrus_communis pycom11g18180 pycom11g18210 pycom11g18230 pycom11g18270 pycom11g18350 pycom11g18360 pycom11g18370
rosa_chinensis RchiOBHm_Chr2g0106511 RchiOBHm_Chr2g0106541 RchiOBHm_Chr2g0106551 RchiOBHm_Chr2g0106571 RchiOBHm_Chr2g0124621 RchiOBHm_Chr6g0251591 RchiOBHm_Chr6g0251631 RchiOBHm_Chr6g0251641
rosa_laevigata RLG00000015443 RLG00000015444 RLG00000015458 RLG00000017510 RLG00000017511 RLG00000017512 RLG00000017515 RLG00000017516 RLG00000017517 RLG00000018809 RLG00000020934 RLG00000020935 RLG00000034092
rosa_multiflora Rmu_co8285777.1_g000001 Rmu_sc0000762.1_g000001 Rmu_sc0001872.1_g000006 Rmu_sc0005255.1_g000019 Rmu_sc0005597.1_g000006 Rmu_sc0012104.1_g000001 Rmu_sc0017105.1_g000011 Rmu_sc0022904.1_g000001 Rmu_ssc0000135.1_g000049 Rmu_ssc0000213.1_g000047
rosa_roxburghii Rroxscaffold_2G00092830 Rroxscaffold_2G00119790 Rroxscaffold_2G00137010 Rroxscaffold_2G00137030 Rroxscaffold_2G00137070 Rroxscaffold_7G00216560
rosa_rugosa Rorug02G0136100 Rorug02G0136200 Rorug02G0136300 Rorug02G0136400 Rorug02G0251800 Rorug02G0251800 Rorug02G0455500 Rorug02G0455600 Rorug02G0455700 Rorug05G0195300
rosa_samantha Rh2AG187400 Rh2AG187500 Rh2AG187600 Rh2AG310500 Rh2BG198200 Rh2BG198300 Rh2BG198400 Rh2BG198600 Rh2BG318900 Rh2BG534300 Rh2CG192400 Rh2CG192500 Rh2CG192600 Rh2CG297500 Rh2DG193500 Rh2DG193600 Rh2DG193800 Rh2DG334200 Rh2DG543100 Rh5AG280400 Rh5BG285800 Rh5BG285900 Rh5CG317900 Rh5CG318000 Rh5DG294600 Rh5DG294700 Rh6AG012200 Rh6AG012700 Rh6BG011200 Rh6BG011700
rosa_wichuraiana Rw0G009380 Rw2G014610 Rw2G014630 Rw2G014640 Rw2G014680 Rw2G025120 Rw2G043000 Rw5G026490 Rw6G001370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1091
AccB7I CCANNNNNTGG 3 cut(s) 440, 457, 1676
AccI GTMKAC 1 cut(s) 1915
AciI CCGC 2 cut(s) 308, 1118
AclWI GGATC 7 cut(s) 336, 1566, 1648, 1661, 1790, 2048, 2061
AcoI YGGCCR 1 cut(s) 1356
AcsI RAATTY 5 cut(s) 469, 1501, 1608, 1757, 2059
AcuI CTGAAG 2 cut(s) 1451, 1813
AcvI CACGTG 1 cut(s) 2078
AfaI GTAC 5 cut(s) 203, 285, 373, 1681, 1687
AfiI CCNNNNNNNGG 5 cut(s) 38, 440, 457, 946, 1676
AflII CTTAAG 2 cut(s) 527, 658
AflIII ACRYGT 3 cut(s) 14, 1080, 2077
AhlI ACTAGT 1 cut(s) 1682
AjiI CACGTC 1 cut(s) 1083
AleI CACNNNNGTG 1 cut(s) 660
AloI GAACNNNNNNTCC 2 cut(s) 1067, 1099
Alw21I GWGCWC 1 cut(s) 1860
Alw26I GTCTC 3 cut(s) 452, 528, 1936
AlwI GGATC 7 cut(s) 336, 1566, 1648, 1661, 1790, 2048, 2061
AoxI GGCC 4 cut(s) 664, 1356, 1447, 1847
ApeKI GCWGC 3 cut(s) 637, 690, 1997
ApoI RAATTY 5 cut(s) 469, 1501, 1608, 1757, 2059
Asp700I GAANNNNTTC 2 cut(s) 8, 1165
AspA2I CCTAGG 1 cut(s) 793
AspS9I GGNCC 6 cut(s) 357, 397, 460, 937, 1280, 1847
AsuC2I CCSGG 2 cut(s) 159, 362
AsuHPI GGTGA 2 cut(s) 422, 1084
AsuII TTCGAA 4 cut(s) 554, 1370, 1719, 1822
AvaII GGWCC 5 cut(s) 357, 397, 460, 937, 1280
AvrII CCTAGG 1 cut(s) 793
AxyI CCTNAGG 1 cut(s) 1087
BalI TGGCCA 1 cut(s) 1358
BamHI GGATCC 1 cut(s) 2053
BanI GGYRCC 1 cut(s) 1091
BauI CACGAG 2 cut(s) 276, 1170
BbrPI CACGTG 1 cut(s) 2078
BbsI GAAGAC 1 cut(s) 701
Bbv12I GWGCWC 1 cut(s) 1860
BbvI GCAGC 3 cut(s) 624, 677, 1984
BccI CCATC 9 cut(s) 518, 611, 1093, 1117, 1141, 1348, 1599, 1670, 1687
BcgI CGANNNNNNTGC 4 cut(s) 116, 150, 1709, 1743
BclI TGATCA 1 cut(s) 775
BcnI CCSGG 2 cut(s) 159, 362
BcoDI GTCTC 3 cut(s) 452, 528, 1936
BcuI ACTAGT 1 cut(s) 1682
BfaI CTAG 9 cut(s) 213, 354, 614, 794, 1313, 1605, 1632, 1683, 1814
BfrI CTTAAG 2 cut(s) 527, 658
BglII AGATCT 2 cut(s) 6, 1245
BisI GCNGC 3 cut(s) 638, 691, 1998
BlnI CCTAGG 1 cut(s) 793
BlsI GCNGC 3 cut(s) 639, 692, 1999
Bme1390I CCNGG 2 cut(s) 159, 362
Bme18I GGWCC 5 cut(s) 357, 397, 460, 937, 1280
BmgBI CACGTC 1 cut(s) 1083
BmgT120I GGNCC 6 cut(s) 357, 397, 460, 937, 1280, 1847
BmiI GGNNCC 5 cut(s) 359, 939, 1093, 1365, 2055
BmrFI CCNGG 2 cut(s) 159, 362
BmsI GCATC 3 cut(s) 87, 262, 814
BoxI GACNNNNGTC 1 cut(s) 536
BpiI GAAGAC 1 cut(s) 701
Bpu10I CCTNAGC 1 cut(s) 933
Bpu14I TTCGAA 4 cut(s) 554, 1370, 1719, 1822
BpuEI CTTGAG 4 cut(s) 688, 1096, 1120, 1873
BpuMI CCSGG 2 cut(s) 159, 362
BsaAI YACGTR 3 cut(s) 1689, 1870, 2078
BsaI GGTCTC 2 cut(s) 452, 1936
BsaJI CCNNGG 4 cut(s) 360, 793, 1359, 1837
BsaWI WCCGGW 1 cut(s) 1945
Bsc4I CCNNNNNNNGG 5 cut(s) 38, 440, 457, 946, 1676
Bse1I ACTGG 3 cut(s) 1162, 1450, 1622
Bse21I CCTNAGG 1 cut(s) 1087
BseDI CCNNGG 4 cut(s) 360, 793, 1359, 1837
BseGI GGATG 6 cut(s) 28, 102, 277, 554, 1130, 1698
BseLI CCNNNNNNNGG 5 cut(s) 38, 440, 457, 946, 1676
BseMII CTCAG 2 cut(s) 947, 1101
BseNI ACTGG 3 cut(s) 1162, 1450, 1622
BseXI GCAGC 3 cut(s) 624, 677, 1984
BshFI GGCC 4 cut(s) 666, 1358, 1449, 1849
BshNI GGYRCC 1 cut(s) 1091
BsiHKAI GWGCWC 1 cut(s) 1860
BsiSI CCGG 3 cut(s) 158, 362, 1946
BslFI GGGAC 3 cut(s) 634, 981, 1215
BslI CCNNNNNNNGG 5 cut(s) 38, 440, 457, 946, 1676
BsmAI GTCTC 3 cut(s) 452, 528, 1936
BsmFI GGGAC 3 cut(s) 634, 981, 1215
BsmI GAATGC 1 cut(s) 196
BsnI GGCC 4 cut(s) 666, 1358, 1449, 1849
Bso31I GGTCTC 2 cut(s) 452, 1936
Bsp119I TTCGAA 4 cut(s) 554, 1370, 1719, 1822
Bsp1286I GDGCHC 1 cut(s) 1860
BspACI CCGC 2 cut(s) 308, 1118
BspANI GGCC 4 cut(s) 666, 1358, 1449, 1849
BspCNI CTCAG 2 cut(s) 946, 1100
BspLI GGNNCC 5 cut(s) 359, 939, 1093, 1365, 2055
BspPI GGATC 7 cut(s) 336, 1566, 1648, 1661, 1790, 2048, 2061
BspT104I TTCGAA 4 cut(s) 554, 1370, 1719, 1822
BspT107I GGYRCC 1 cut(s) 1091
BspTI CTTAAG 2 cut(s) 527, 658
BspTNI GGTCTC 2 cut(s) 452, 1936
BsrI ACTGG 3 cut(s) 1162, 1450, 1622
BssECI CCNNGG 4 cut(s) 360, 793, 1359, 1837
BssSI CACGAG 2 cut(s) 276, 1170
BssT1I CCWWGG 2 cut(s) 793, 1359
Bst2BI CACGAG 2 cut(s) 276, 1170
Bst4CI ACNGT 7 cut(s) 93, 133, 237, 376, 986, 995, 1884
BstAFI CTTAAG 2 cut(s) 527, 658
BstAPI GCANNNNNTGC 1 cut(s) 298
BstBAI YACGTR 3 cut(s) 1689, 1870, 2078
BstBI TTCGAA 4 cut(s) 554, 1370, 1719, 1822
BstC8I GCNNGC 1 cut(s) 930
BstDEI CTNAG 6 cut(s) 535, 668, 683, 933, 1087, 1746
BstDSI CCRYGG 1 cut(s) 1837
BstF5I GGATG 6 cut(s) 28, 102, 277, 554, 1130, 1698
BstMAI GTCTC 3 cut(s) 452, 528, 1936
BstMWI GCNNNNNNNGC 2 cut(s) 298, 1855
BstNSI RCATGY 1 cut(s) 18
BstPAI GACNNNNGTC 1 cut(s) 536
BstSCI CCNGG 2 cut(s) 157, 360
BstV1I GCAGC 3 cut(s) 624, 677, 1984
BstV2I GAAGAC 1 cut(s) 701
BstX2I RGATCY 6 cut(s) 6, 328, 1245, 1640, 1795, 2053
BstXI CCANNNNNNTGG 2 cut(s) 517, 1844
BstYI RGATCY 6 cut(s) 6, 328, 1245, 1640, 1795, 2053
Bsu36I CCTNAGG 1 cut(s) 1087
BsuRI GGCC 4 cut(s) 666, 1358, 1449, 1849
BtgI CCRYGG 1 cut(s) 1837
BtgZI GCGATG 1 cut(s) 1583
BtrI CACGTC 1 cut(s) 1083
BtsCI GGATG 6 cut(s) 28, 102, 277, 554, 1130, 1698
Cac8I GCNNGC 1 cut(s) 930
Cfr13I GGNCC 6 cut(s) 357, 397, 460, 937, 1280, 1847
Csp6I GTAC 5 cut(s) 202, 284, 372, 1680, 1686
CviAII CATG 7 cut(s) 15, 101, 289, 589, 1054, 1198, 1307
CviQI GTAC 5 cut(s) 202, 284, 372, 1680, 1686
DdeI CTNAG 6 cut(s) 535, 668, 683, 933, 1087, 1746
DraI TTTAAA 3 cut(s) 1500, 1530, 1762
EaeI YGGCCR 1 cut(s) 1356
Eco130I CCWWGG 2 cut(s) 793, 1359
Eco31I GGTCTC 2 cut(s) 452, 1936
Eco32I GATATC 3 cut(s) 744, 1432, 1516
Eco47I GGWCC 5 cut(s) 357, 397, 460, 937, 1280
Eco57I CTGAAG 2 cut(s) 1451, 1813
Eco72I CACGTG 1 cut(s) 2078
Eco81I CCTNAGG 1 cut(s) 1087
EcoO109I RGGNCCY 2 cut(s) 357, 937
EcoRI GAATTC 1 cut(s) 1608
EcoRV GATATC 3 cut(s) 744, 1432, 1516
EcoT14I CCWWGG 2 cut(s) 793, 1359
EcoT22I ATGCAT 2 cut(s) 102, 294
ErhI CCWWGG 2 cut(s) 793, 1359
FaeI CATG 7 cut(s) 18, 104, 292, 592, 1057, 1201, 1310
FalI AAGNNNNNCTT 2 cut(s) 1785, 1817
FaqI GGGAC 3 cut(s) 634, 981, 1215
FatI CATG 7 cut(s) 14, 100, 288, 588, 1053, 1197, 1306
FbaI TGATCA 1 cut(s) 775
FblI GTMKAC 1 cut(s) 1915
Fnu4HI GCNGC 3 cut(s) 638, 691, 1998
FokI GGATG 6 cut(s) 15, 109, 284, 561, 1117, 1705
Fsp4HI GCNGC 3 cut(s) 638, 691, 1998
FspBI CTAG 9 cut(s) 213, 354, 614, 794, 1313, 1605, 1632, 1683, 1814
GluI GCNGC 3 cut(s) 638, 691, 1998
HaeIII GGCC 4 cut(s) 666, 1358, 1449, 1849
HapII CCGG 3 cut(s) 158, 362, 1946
Hin1II CATG 7 cut(s) 18, 104, 292, 592, 1057, 1201, 1310
HincII GTYRAC 2 cut(s) 49, 966
HindII GTYRAC 2 cut(s) 49, 966
HindIII AAGCTT 2 cut(s) 383, 715
HinfI GANTC 6 cut(s) 506, 521, 1127, 1166, 1295, 1563
HpaII CCGG 3 cut(s) 158, 362, 1946
HphI GGTGA 2 cut(s) 422, 1084
Hpy166II GTNNAC 8 cut(s) 49, 140, 164, 430, 767, 966, 1075, 1916
Hpy188I TCNGA 5 cut(s) 424, 604, 741, 1039, 1666
Hpy8I GTNNAC 8 cut(s) 49, 140, 164, 430, 767, 966, 1075, 1916
Hpy99I CGWCG 1 cut(s) 909
HpyAV CCTTC 7 cut(s) 74, 246, 398, 509, 864, 1051, 1377
HpyCH4III ACNGT 7 cut(s) 93, 133, 237, 376, 986, 995, 1884
HpyCH4IV ACGT 6 cut(s) 166, 1082, 1330, 1688, 1869, 2077
HpyCH4V TGCA 6 cut(s) 100, 292, 693, 814, 1327, 2000
HpyF10VI GCNNNNNNNGC 2 cut(s) 298, 1855
HpyF3I CTNAG 6 cut(s) 535, 668, 683, 933, 1087, 1746
HpySE526I ACGT 6 cut(s) 166, 1082, 1330, 1688, 1869, 2077
Hsp92II CATG 7 cut(s) 18, 104, 292, 592, 1057, 1201, 1310
Ksp22I TGATCA 1 cut(s) 775
LmnI GCTCC 2 cut(s) 634, 798
Lsp1109I GCAGC 3 cut(s) 624, 677, 1984
LweI GCATC 3 cut(s) 87, 262, 814
MaeI CTAG 9 cut(s) 213, 354, 614, 794, 1313, 1605, 1632, 1683, 1814
MaeII ACGT 6 cut(s) 166, 1082, 1330, 1688, 1869, 2077
MaeIII GTNAC 7 cut(s) 20, 911, 1153, 1250, 1648, 1832, 2083
MfeI CAATTG 2 cut(s) 438, 779
MflI RGATCY 6 cut(s) 6, 328, 1245, 1640, 1795, 2053
MhlI GDGCHC 1 cut(s) 1860
MlsI TGGCCA 1 cut(s) 1358
MluCI AATT 9 cut(s) 75, 438, 469, 779, 1501, 1608, 1757, 1929, 2059
MluNI TGGCCA 1 cut(s) 1358
MlyI GAGTC 2 cut(s) 500, 530
MmeI TCCRAC 2 cut(s) 475, 582
Mox20I TGGCCA 1 cut(s) 1358
Mph1103I ATGCAT 2 cut(s) 102, 294
MroXI GAANNNNTTC 2 cut(s) 8, 1165
MscI TGGCCA 1 cut(s) 1358
MslI CAYNNNNRTG 3 cut(s) 660, 973, 1425
Msp20I TGGCCA 1 cut(s) 1358
MspA1I CMGCKG 1 cut(s) 2050
MspCI CTTAAG 2 cut(s) 527, 658
MspI CCGG 3 cut(s) 158, 362, 1946
MspR9I CCNGG 2 cut(s) 159, 362
MunI CAATTG 2 cut(s) 438, 779
Mva1269I GAATGC 1 cut(s) 196
MwoI GCNNNNNNNGC 2 cut(s) 298, 1855
NciI CCSGG 2 cut(s) 159, 362
NlaIII CATG 7 cut(s) 18, 104, 292, 592, 1057, 1201, 1310
NlaIV GGNNCC 5 cut(s) 359, 939, 1093, 1365, 2055
NmuCI GTSAC 2 cut(s) 20, 1250
NsiI ATGCAT 2 cut(s) 102, 294
NspI RCATGY 1 cut(s) 18
NspV TTCGAA 4 cut(s) 554, 1370, 1719, 1822
OliI CACNNNNGTG 1 cut(s) 660
PciI ACATGT 1 cut(s) 14
PctI GAATGC 1 cut(s) 196
PdmI GAANNNNTTC 2 cut(s) 8, 1165
PfeI GAWTC 4 cut(s) 1127, 1166, 1295, 1563
PflMI CCANNNNNTGG 3 cut(s) 440, 457, 1676
PkrI GCNGC 3 cut(s) 639, 692, 1999
PleI GAGTC 2 cut(s) 500, 529
PmaCI CACGTG 1 cut(s) 2078
PmlI CACGTG 1 cut(s) 2078
PpsI GAGTC 2 cut(s) 500, 529
Ppu21I YACGTR 3 cut(s) 1689, 1870, 2078
PpuMI RGGWCCY 2 cut(s) 357, 937
PscI ACATGT 1 cut(s) 14
PshAI GACNNNNGTC 1 cut(s) 536
Psp5II RGGWCCY 2 cut(s) 357, 937
PspCI CACGTG 1 cut(s) 2078
PspN4I GGNNCC 5 cut(s) 359, 939, 1093, 1365, 2055
PspPI GGNCC 6 cut(s) 357, 397, 460, 937, 1280, 1847
PspPPI RGGWCCY 2 cut(s) 357, 937
PsuI RGATCY 6 cut(s) 6, 328, 1245, 1640, 1795, 2053
PvuII CAGCTG 1 cut(s) 2050
RsaI GTAC 5 cut(s) 203, 285, 373, 1681, 1687
RsaNI GTAC 5 cut(s) 202, 284, 372, 1680, 1686
RseI CAYNNNNRTG 3 cut(s) 660, 973, 1425
SatI GCNGC 3 cut(s) 638, 691, 1998
Sau96I GGNCC 6 cut(s) 357, 397, 460, 937, 1280, 1847
SchI GAGTC 2 cut(s) 500, 530
ScrFI CCNGG 2 cut(s) 159, 362
SduI GDGCHC 1 cut(s) 1860
SfaNI GCATC 3 cut(s) 87, 262, 814
SfuI TTCGAA 4 cut(s) 554, 1370, 1719, 1822
SinI GGWCC 5 cut(s) 357, 397, 460, 937, 1280
SmiI ATTTAAAT 2 cut(s) 1500, 1530
SmiMI CAYNNNNRTG 3 cut(s) 660, 973, 1425
SmlI CTYRAG 6 cut(s) 527, 658, 703, 1111, 1135, 1888
SmoI CTYRAG 6 cut(s) 527, 658, 703, 1111, 1135, 1888
SpeI ACTAGT 1 cut(s) 1682
Sse9I AATT 9 cut(s) 75, 438, 469, 779, 1501, 1608, 1757, 1929, 2059
SsiI CCGC 2 cut(s) 308, 1118
SspI AATATT 1 cut(s) 1492
SspMI CTAG 9 cut(s) 213, 354, 614, 794, 1313, 1605, 1632, 1683, 1814
StyD4I CCNGG 2 cut(s) 157, 360
StyI CCWWGG 2 cut(s) 793, 1359
SwaI ATTTAAAT 2 cut(s) 1500, 1530
TaaI ACNGT 7 cut(s) 93, 133, 237, 376, 986, 995, 1884
TaiI ACGT 6 cut(s) 169, 1085, 1333, 1691, 1872, 2080
TaqI TCGA 8 cut(s) 402, 554, 907, 1370, 1518, 1561, 1719, 1822
TasI AATT 9 cut(s) 75, 438, 469, 779, 1501, 1608, 1757, 1929, 2059
TatI WGTACW 2 cut(s) 283, 371
TfiI GAWTC 4 cut(s) 1127, 1166, 1295, 1563
TseFI GTSAC 2 cut(s) 20, 1250
TseI GCWGC 3 cut(s) 637, 690, 1997
Tsp45I GTSAC 2 cut(s) 20, 1250
TspDTI ATGAA 9 cut(s) 17, 17, 709, 995, 1070, 1119, 1330, 1586, 2033
TspGWI ACGGA 2 cut(s) 893, 1952
Van91I CCANNNNNTGG 3 cut(s) 440, 457, 1676
Vha464I CTTAAG 2 cut(s) 527, 658
VpaK11BI GGWCC 5 cut(s) 357, 397, 460, 937, 1280
XapI RAATTY 5 cut(s) 469, 1501, 1608, 1757, 2059
XbaI TCTAGA 1 cut(s) 613
XceI RCATGY 1 cut(s) 18
XmaJI CCTAGG 1 cut(s) 793
XmiI GTMKAC 1 cut(s) 1915
XmnI GAANNNNTTC 2 cut(s) 8, 1165
XspI CTAG 9 cut(s) 213, 354, 614, 794, 1313, 1605, 1632, 1683, 1814
Zsp2I ATGCAT 2 cut(s) 102, 294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.