RH2AG187600

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: Sequence Only
Biological Identity
rosa_samantha
Unknown
OG0000307
Physical Location & Seq
Reverse (-)
0 .. 0
1 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG187600.1

Sequence Viewer

Length: 4860 bp
ATGGAACCACCTTCATCATCTTCCTCCCCCAACCCCCAAACAATTCCTTCATCCTCTAACTGGGAACATGATGTCTTCCTCAGTTTCAGGGGCGAGGACACTCGCAATAGTTTTACAGACCATTTATACTATGCTATGAGCCAGAGAGGAATCGACACATTTAGAGACACTGAAAAGCTTCGGAGGGGGAAATCCATTTCACCAGAACTTCTGAAAGCAATCGAAGAGTCCAAGTTTGCGGTTACAGTTCTTTCAACCAACTATGCTACTTCAACTTGGTGTCTGGATGAACTTGCCCATATTCTCGATTGCAGGAAAGTAAGGGGACTGGAAGTTCTTCCGGTTTTTTATCATGTAGAACCATCTGAGATAAGGAAGCAAACTGGAAACTACGGCAAGGCGTTTGCTAAGCATGAGACAGATTTCAAGGAAAATATGAGAAAGGTGGACAAGTGGAGGAAAGCTTTAGAAGATATAGCCAGTCTCTCCGGATGGCATGTGACACAGGATAGGGGAGAATCGGAAGTTATACAGGAAATAGCTAAAGAGATTTCAATATGTTTGAACAACACATTATCCAATCCTGACAGAGATTTGATTGGAATGGATGTGCGTATAGAAAAGTTGGAGTCTTACTTGGATCTAAGGTCGGATGACGTTCTTAGCATAGGGATTTGGGGGATGGGGGGCATTGGTAAGACAACTCTGGCAAAAGAAGTTTTCAAAAAGATTCGTAACCAATTTGATAGGAGCGGCTTTCTTTCCAATGTTAGATTGCATTCCGAAGAAAGAGCTCTAGTTGACTTGCAAAAACTTCTGTGTGGATCCTCTTTCCAGGACAACAACATAAATATAGACACTGTTGGAGTAGGGATCAGGTTGTTAAAGAAAAGACTACTTAAGAAAAAGGTGCTTATTGTTCTAGATGATGTTGATGAATTAAAACAACTGAAAGATTTAGCACCTGGAAGGCAGAATGAACGGTACTCTTGGGGCCGAGGGAGTCGACTCATAATAACAACTAGGGATAGGCGTCCATTGATAGAATGTGAAGTACATAAAATATATGAGGCTGAAAAACTTAGTGATGAGGAAGCTTCTCAGCTCATATGTCAACAAGCCTTCAAGAAAAACTATCCACCAGCTGAGTTTGTAGAGCTGTCCAAGAGTTTTGTAAAATATGCCGGTGGCCTTCCTCTAGCTCATAAAGTTAAGGGTTCACAATTGTGGGGAAGGGAGGTAGATGAATGGTCAGAGGTATTAGATAGACTGGACGAAGATCTGGATAAAGACATTTTTAGTGTGCTTGAAATAAGTTTTGATGGATTACAGGATACAGATAAGAAAATCTTTTTGGACATCGCATGTTTCTTCAATGGTGAGGATCATGTTCGTGTACAAAAGATATTGAAAAGTTGTGGTTTTTCTCCTCGAATAGGCATAACTAACCTCATTGACAAATCTCTGATCAAAATTGAAAGTGAAAAACTGTGGATGCATGAATTACTACGGTGTTTGGGTTGGCATATTGTTCGCAGAGAATCAAAACCTTTTCCGGGCAAACGTAGCAGATTGTGGCTTGATGATAATGCACACAAGTACAAAGGCAGAAGGCCATGGCACTTTGAGGATGCTCGTAATGTACTCGCGAGTAATACCGGAACATCTGCTGTTGAAGGCTTATTCTTAAGCTTGGGTGAAAAAGAAGAAATGCGTTTGAGCAATGACCCATTCTTAACTATGACCAAACTAAGATTGCTGAAGATTTATAATGTAAATTTTCTGGGTGGGCACTTTACATATCTCTCTGACGATTTACGTTGTCTGGAATGGCACGAATGTCCTCTAAAGTCTCTGCCATCTAGTTTTACGCCCTATCAGCTGGTTGAATTCAAGATGCCTAACAGCTGCATTGAACGACTGTGGAAGAAAACGCCTTCTATGAGAATGCTGACACTCATGGATCTTTCCAACTGCCAATATCTAGTCACGACCCCTGACTTCGGTGAGGTCCCAAATCTTGAGAGATTGATCCTTGAAGGTTGTAAAGAGTTATCCGCGGTTCACCCAACAATTAGGGATCTCCAGCATCTGATTTTATTGAATTTGAAAGGCTGTGCAAGTCTAAAGAGCCTTCCCCCATCAATCAGCTTGAGATCTCTTCAAACTTTTATTCTTTCGGGATGTTCAAAACTGGAAGAGTTTCCGGAGATTGTGGGAAACATGCAAACTTTGTCAGAACTTTATTTAGATGGCACCGCTATTAGGGAGGTGCCTGTATCAATCCAGCTTTTAACAGGCCTTGTTTTGCTAAATCTATGTGGCTGCAAGAACCTTCCGAGTCTTCCAAGCGCTCTTTGTAGAAGTTTGACATCACTGAAATTTCTTTATCTGTCAAGGTGCTCTAGTTTGGACAAACTGCCAGAAAACATAGGCTCCTTGGAACACTTGGAGGAGCTTGATGCATGTCATACTGCTATAAGAAAAGTGCCCGAGTCCATTTCACTACTCAAGAATCTTAAACTACTGTGTTTCCATGGCTGCTCTGGATACACAGGTCTAGAGATGCCAAACAAGTTCTCAGGTCTAAGATGTTTGACAACACTAAACCTAGGTGGATGTAATCTCCCTGAAGGAGCAATCCCCAGTGACATTGGCAATTTGTTCTCACTGCAGAGTCTGGATTTAAGTGAAAACAACTTTTTCACCATACCTGAAAGCATCTCTCAACTTTCTGAGCTTACAGAAATTTTTCTGTTTAGGTGTAGTAAACTACAATCGTTGCCAAAAGATCTTCCATCAAGACTAAGAACTATAAATGTACGGGACTGTCCTATGCTGACAGATTTTTCATATAATTTGATGAGATATCCACCTCGAAAGGGTTTAAGTACTATCATAAGTTGTCGAAAACCGGAGGAGGATGAGCAGTTGCCAATATTGCTACCTGAACTCGATGAGGTGTCTCTTTCTCTGCAGTTGCCAATGCTACCTGAACTCCATGAGTTTCGTTTGGGAAATCTGGAAAAACTCGATCTTTCCTACTGCCAACACTTGAAGAAGATCCCTGACTTGAATGGGGTTCCAAACCTTAAGAAATTGATCCTTGAAGGCTGTGAAAAATTATCAGAGGTTCACCCAACATTTGGGAGTCTCCAACATCTGATTTTTTTGAATATGAAAGGATGTGTAGATCTAGAGAGCCTTCCCCGCTTCATCTGCATGAAATATCTTGAAATCTGTATTCTTTCAGGATGTTCAAAGCTTAAAGAGTTTCCGGAGATTAATGGAGATATGGATAAATTGTCACAACTGCATTTAGATGGGACGGCTTTAGAGAATCTGCCGATACCAATGCAGCATTTGAAATGCCCTATTGTGATAAATCTAAGAGGTTGCAAGAACCTATTGACTTTTCCAATCCTTTTGAGTCTGAAAGCTCTCAATCTGTCAGGCTGCTCTCGTATATCCAGATTTCCAAATAACTTGGCAAGCATGGCACATTTGGAGGAGCTTGATGCCTCTGAAACTGCTATAACACGAGTACCCCAGTCCATTTCATTTATGAAGAAGCTTAAAGTGTTGTCTTTCTGTGGATGTAAAGGTTTGCAGTTGCCTAACCAGTTCTTGCAATTAAGCTTTTTGACATCATTAAATCTACGGAGGTGTGGTCTAGCAGAAATAACAGTCCTTGCTAGCCTCTGTGGCTTATCCTCACTGCAAAAGTTGGATTTGAGTGGAAACAAATTTGTGAGTATACCTATTGAAATTAGTCGCTTATCCTCTTTGAAGCAATTGAATTTGAGTAGGAACGACTTGGTGAGTGTACCAGATGCAATTGGTCGCTTATCCTCTTTGCAACAATTGAATTTGAGTATGAACAACTTGGTGAGTATACCAGATGCAATTGATCGACTTTCCTCTTTGCAGCAATTGGATTTGAGTACGAACGACTTGGTGAGTATACCAGATGCAATTGGTGGCTTATCCTCTTTGCAGCGATTGAATTTGAGTACGAACGACTTGGTGAGTATACCAGATGCAATTGGTGGCTTATCCTCTTTGCAGCGATTGGATTTGAGTACGAACGACTTGGTGAGTATACCAGATGCAATTGGTCGACTTTCCTCTTTGCAGCGATTGGATTTGAGTATGAACGACTTGGTGAGTATACCAGATGCAACTGGTGGCTTATCCTCTTTGCAGCGATTGGATTTGAGTATGAACGACTTGGTGAGTATACCAGATGCAACTGGTGGCTTATCCTCTTTGCAGCGATTGGATTTGAGTATGAACGACTTGGTGAGTATACCAGATGCAATTGGTGGCTTATCCTCTTTGCAGCGATTGGATTTGAGTACGAACGACTTGGTGAGTATACCAGATGCAATTGGTCGACTTTCCTCTTTGCAGCGATTGGATTTGAGTATGAACGACTTGGTGAGTATACCAGATGCAACTGGTGGCTTATCCTCTTTGCAGCGATTGGATTTGAGTACGAACGACTTGGTGAGTATACCAGATGCAACTGGTGGCTTATCCTCTTTGCAGCGATTGGATTTGAGTATGAACGACTTGGTGAGTATACCAGATGCAATTGGTGGCTTATCCTCTTTGCAGCGATTGGATTTGAGTACGAACGACTTGGTGAGTATACCAGATGCAATTGGTCGACTTTCCTCTTTGCAGCGATTGGATTTGAGTATGAACGACTTGGTGAGTATACCAGATGCAATTGGTCGACTCTCCTCGTTGGAGCGATTGGATTTGAGTAGGAACAAGCTGGTGAGCATACCAGATGCAATCGGTCTCTTAACATCCTTGAAGCATTTAGATTGGAGTGAAAACAACTCTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 LRR_8 NB-ARC TIR

Protein Analysis

1619

Amino Acids

180.73

Weight (kDa)

5.78

Isoelectric Point (pI)

41.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 23 - 194 3.7e-55 TIR domain
TIR_2 PF13676 25 - 122 9.8e-15 TIR domain
NB-ARC PF00931 206 - 378 1e-19 NB-ARC domain
NACHT PF05729 225 - 372 1.2e-06 NACHT domain
WHD_ROQ1 PF23282 446 - 515 1.4e-19 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 718 - 787 1.1e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 770 - 871 2e-12 Leucine-rich repeat region
LRR_13 PF23286 806 - 950 5.1e-07 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 1133 - 1245 2.4e-08 Leucine-rich repeat region
LRR_14 PF23598 1212 - 1335 5.8e-12 Leucine-rich repeat region
LRR_8 PF13855 1215 - 1273 3.6e-06 Leucine rich repeat
LRR_8 PF13855 1262 - 1319 1.4e-09 Leucine rich repeat
LRR_8 PF13855 1323 - 1365 5.9e-08 Leucine rich repeat
LRR_14 PF23598 1325 - 1430 3.9e-11 Leucine-rich repeat region
LRR_8 PF13855 1370 - 1411 2.3e-06 Leucine rich repeat
LRR_14 PF23598 1371 - 1455 2e-08 Leucine-rich repeat region
LRR_14 PF23598 1398 - 1476 2.7e-08 Leucine-rich repeat region
LRR_8 PF13855 1400 - 1457 3.3e-10 Leucine rich repeat
LRR_8 PF13855 1423 - 1480 2e-09 Leucine rich repeat
LRR_8 PF13855 1462 - 1503 1.4e-06 Leucine rich repeat
LRR_14 PF23598 1464 - 1545 4.9e-08 Leucine-rich repeat region
LRR_8 PF13855 1469 - 1526 5.1e-10 Leucine rich repeat
LRR_8 PF13855 1492 - 1549 1.1e-10 Leucine rich repeat
LRR_8 PF13855 1515 - 1572 2e-09 Leucine rich repeat
LRR_14 PF23598 1533 - 1613 1.3e-10 Leucine-rich repeat region
LRR_8 PF13855 1561 - 1617 1.6e-08 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000307)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g16600 FvH4_1g16610 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16621 FvH4_1g16630 FvH4_1g16640 FvH4_1g16640 FvH4_1g16650 FvH4_1g16650 FvH4_3g27631 FvH4_4g11392 FvH4_4g11542
prunus_persica Prupe.4G224500_v2.0.a1 Prupe.4G226900_v2.0.a1 Prupe.4G227000_v2.0.a1
pyrus_communis pycom11g18180 pycom11g18210 pycom11g18230 pycom11g18270 pycom11g18350 pycom11g18360 pycom11g18370
rosa_chinensis RchiOBHm_Chr2g0106511 RchiOBHm_Chr2g0106541 RchiOBHm_Chr2g0106551 RchiOBHm_Chr2g0106571 RchiOBHm_Chr2g0124621 RchiOBHm_Chr6g0251591 RchiOBHm_Chr6g0251631 RchiOBHm_Chr6g0251641
rosa_laevigata RLG00000015443 RLG00000015444 RLG00000015458 RLG00000017510 RLG00000017511 RLG00000017512 RLG00000017515 RLG00000017516 RLG00000017517 RLG00000018809 RLG00000020934 RLG00000020935 RLG00000034092
rosa_multiflora Rmu_co8285777.1_g000001 Rmu_sc0000762.1_g000001 Rmu_sc0001872.1_g000006 Rmu_sc0005255.1_g000019 Rmu_sc0005597.1_g000006 Rmu_sc0012104.1_g000001 Rmu_sc0017105.1_g000011 Rmu_sc0022904.1_g000001 Rmu_ssc0000135.1_g000049 Rmu_ssc0000213.1_g000047
rosa_roxburghii Rroxscaffold_2G00092830 Rroxscaffold_2G00119790 Rroxscaffold_2G00137010 Rroxscaffold_2G00137030 Rroxscaffold_2G00137070 Rroxscaffold_7G00216560
rosa_rugosa Rorug02G0136100 Rorug02G0136200 Rorug02G0136300 Rorug02G0136400 Rorug02G0251800 Rorug02G0251800 Rorug02G0455500 Rorug02G0455600 Rorug02G0455700 Rorug05G0195300
rosa_samantha Rh2AG187400 Rh2AG187500 Rh2AG187600 Rh2AG310500 Rh2BG198200 Rh2BG198300 Rh2BG198400 Rh2BG198600 Rh2BG318900 Rh2BG534300 Rh2CG192400 Rh2CG192500 Rh2CG192600 Rh2CG297500 Rh2DG193500 Rh2DG193600 Rh2DG193800 Rh2DG334200 Rh2DG543100 Rh5AG280400 Rh5BG285800 Rh5BG285900 Rh5CG317900 Rh5CG318000 Rh5DG294600 Rh5DG294700 Rh6AG012200 Rh6AG012700 Rh6BG011200 Rh6BG011700
rosa_wichuraiana Rw0G009380 Rw2G014610 Rw2G014630 Rw2G014640 Rw2G014680 Rw2G025120 Rw2G043000 Rw5G026490 Rw6G001370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1770
AccB1I GGYRCC 2 cut(s) 2255, 2272
AccB7I CCANNNNNTGG 1 cut(s) 3146
AccBSI CCGCTC 1 cut(s) 753
AccII CGCG 2 cut(s) 1649, 2060
AccIII TCCGGA 3 cut(s) 488, 2206, 3277
AciI CCGC 6 cut(s) 239, 753, 2058, 2060, 2259, 3211
AcsI RAATTY 9 cut(s) 1777, 1889, 2104, 2381, 2748, 3746, 3799, 3868, 4006
AcuI CTGAAG 2 cut(s) 1781, 2652
AcyI GRCGYC 1 cut(s) 1033
AfeI AGCGCT 1 cut(s) 2353
AfiI CCNNNNNNNGG 7 cut(s) 60, 1436, 1556, 2003, 2265, 2882, 3146
AflII CTTAAG 3 cut(s) 899, 1687, 3092
AjnI CCWGG 2 cut(s) 834, 964
AjuI GAANNNNNNNTTGG 2 cut(s) 1337, 1369
AleI CACNNNNGTG 1 cut(s) 1225
AloI GAACNNNNNNTCC 2 cut(s) 318, 350
Alw21I GWGCWC 2 cut(s) 796, 2405
Alw26I GTCTC 7 cut(s) 159, 410, 488, 1857, 2970, 3158, 4814
AlwNI CAGNNNCTG 2 cut(s) 2092, 2680
Ama87I CYCGRG 1 cut(s) 2492
Aor13HI TCCGGA 3 cut(s) 488, 2206, 3277
Aor51HI AGCGCT 1 cut(s) 2353
AoxI GGCC 4 cut(s) 994, 1189, 1613, 2299
ApoI RAATTY 9 cut(s) 1777, 1889, 2104, 2381, 2748, 3746, 3799, 3868, 4006
AseI ATTAAT 1 cut(s) 3285
Asp700I GAANNNNTTC 4 cut(s) 177, 336, 2202, 2751
AspA2I CCTAGG 1 cut(s) 2611
AspLEI GCGC 1 cut(s) 2354
AspS9I GGNCC 2 cut(s) 994, 2011
AsuC2I CCSGG 1 cut(s) 1557
AsuNHI GCTAGC 1 cut(s) 3695
AvaI CYCGRG 1 cut(s) 2492
AvaII GGWCC 1 cut(s) 2011
AvrII CCTAGG 1 cut(s) 2611
BaeGI GKGCMC 2 cut(s) 1794, 2493
BaeI ACNNNNGTAYC 4 cut(s) 2542, 2575, 3528, 3561
BamHI GGATCC 1 cut(s) 824
BanI GGYRCC 2 cut(s) 2255, 2272
BanII GRGCYC 1 cut(s) 796
BauI CACGAG 1 cut(s) 3540
BbsI GAAGAC 2 cut(s) 67, 2336
Bbv12I GWGCWC 2 cut(s) 796, 2405
BccI CCATC 9 cut(s) 370, 486, 676, 1316, 1867, 2149, 2246, 2806, 3317
BceAI ACGGC 2 cut(s) 409, 3345
BcgI CGANNNNNNTGC 4 cut(s) 1514, 1548, 3337, 3371
BciT130I CCWGG 2 cut(s) 836, 966
BciVI GTATCC 2 cut(s) 1327, 2543
BclI TGATCA 1 cut(s) 1467
BcnI CCSGG 1 cut(s) 1557
BcoDI GTCTC 7 cut(s) 159, 410, 488, 1857, 2970, 3158, 4814
BfmI CTRYAG 2 cut(s) 2672, 2975
BfoI RGCGCY 1 cut(s) 2355
BfrI CTTAAG 3 cut(s) 899, 1687, 3092
BfuI GTATCC 2 cut(s) 1327, 2543
BglI GCCNNNNNGGC 1 cut(s) 3705
BglII AGATCT 4 cut(s) 1279, 2156, 2791, 3193
BlnI CCTAGG 1 cut(s) 2611
BlpI GCTNAGC 1 cut(s) 408
BmcAI AGTACT 1 cut(s) 2893
Bme1390I CCNGG 3 cut(s) 836, 966, 1557
Bme18I GGWCC 1 cut(s) 2011
BmeT110I CYCGRG 1 cut(s) 2492
BmgT120I GGNCC 2 cut(s) 994, 2011
BmiI GGNNCC 8 cut(s) 6, 826, 995, 2013, 2257, 2274, 2437, 3084
BmrFI CCNGG 3 cut(s) 836, 966, 1557
BmrI ACTGGG 3 cut(s) 70, 2640, 3544
BmtI GCTAGC 1 cut(s) 3699
BmuI ACTGGG 3 cut(s) 70, 2640, 3544
BpiI GAAGAC 2 cut(s) 67, 2336
BpmI CTGGAG 1 cut(s) 2069
Bpu1102I GCTNAGC 1 cut(s) 408
BpuEI CTTGAG 3 cut(s) 2042, 2173, 2495
BpuMI CCSGG 1 cut(s) 1557
BsaHI GRCGYC 1 cut(s) 1033
BsaI GGTCTC 1 cut(s) 4814
BsaJI CCNNGG 6 cut(s) 997, 1616, 2058, 2439, 2536, 2611
BsaWI WCCGGW 6 cut(s) 340, 488, 1658, 2206, 2914, 3277
BsaXI ACNNNNNCTCC 6 cut(s) 2420, 2450, 2982, 3012, 3655, 3685
Bsc4I CCNNNNNNNGG 7 cut(s) 60, 1436, 1556, 2003, 2265, 2882, 3146
Bse118I RCCGGY 1 cut(s) 1184
Bse3DI GCAATG 1 cut(s) 1729
BseAI TCCGGA 3 cut(s) 488, 2206, 3277
BseBI CCWGG 2 cut(s) 836, 966
BseDI CCNNGG 6 cut(s) 997, 1616, 2058, 2439, 2536, 2611
BseLI CCNNNNNNNGG 7 cut(s) 60, 1436, 1556, 2003, 2265, 2882, 3146
BseMI GCAATG 1 cut(s) 1729
BseMII CTCAG 7 cut(s) 94, 357, 1115, 1137, 2595, 2727, 4845
BseRI GAGGAG 5 cut(s) 1419, 2468, 2933, 3524, 4738
BseSI GKGCMC 2 cut(s) 1794, 2493
Bsh1236I CGCG 2 cut(s) 1649, 2060
BshFI GGCC 4 cut(s) 996, 1191, 1615, 2301
BshNI GGYRCC 2 cut(s) 2255, 2272
BsiHKAI GWGCWC 2 cut(s) 796, 2405
BsiHKCI CYCGRG 1 cut(s) 2492
BsiSI CCGG 8 cut(s) 341, 489, 1185, 1556, 1659, 2207, 2915, 3278
BslFI GGGAC 4 cut(s) 339, 1997, 2840, 3340
BslI CCNNNNNNNGG 7 cut(s) 60, 1436, 1556, 2003, 2265, 2882, 3146
BsmAI GTCTC 7 cut(s) 159, 410, 488, 1857, 2970, 3158, 4814
BsmFI GGGAC 4 cut(s) 339, 1997, 2840, 3340
BsmI GAATGC 2 cut(s) 778, 1953
BsnI GGCC 4 cut(s) 996, 1191, 1615, 2301
Bso31I GGTCTC 1 cut(s) 4814
BsoBI CYCGRG 1 cut(s) 2492
Bsp1286I GDGCHC 4 cut(s) 796, 1794, 2405, 2493
Bsp13I TCCGGA 3 cut(s) 488, 2206, 3277
Bsp1407I TGTACA 1 cut(s) 1396
Bsp1720I GCTNAGC 1 cut(s) 408
Bsp19I CCATGG 2 cut(s) 1616, 2536
Bsp68I TCGCGA 1 cut(s) 1649
BspACI CCGC 6 cut(s) 239, 753, 2058, 2060, 2259, 3211
BspANI GGCC 4 cut(s) 996, 1191, 1615, 2301
BspCNI CTCAG 7 cut(s) 93, 358, 1114, 1138, 2594, 2728, 4846
BspEI TCCGGA 3 cut(s) 488, 2206, 3277
BspFNI CGCG 2 cut(s) 1649, 2060
BspLI GGNNCC 8 cut(s) 6, 826, 995, 2013, 2257, 2274, 2437, 3084
BspMAI CTGCAG 2 cut(s) 2676, 2979
BspOI GCTAGC 1 cut(s) 3699
BspT107I GGYRCC 2 cut(s) 2255, 2272
BspTI CTTAAG 3 cut(s) 899, 1687, 3092
BspTNI GGTCTC 1 cut(s) 4814
BsrBI CCGCTC 1 cut(s) 753
BsrDI GCAATG 1 cut(s) 1729
BsrFI RCCGGY 1 cut(s) 1184
BsrGI TGTACA 1 cut(s) 1396
BssAI RCCGGY 1 cut(s) 1184
BssECI CCNNGG 6 cut(s) 997, 1616, 2058, 2439, 2536, 2611
BssNI GRCGYC 1 cut(s) 1033
BssSI CACGAG 1 cut(s) 3540
BssT1I CCWWGG 4 cut(s) 1616, 2439, 2536, 2611
Bst2BI CACGAG 1 cut(s) 3540
Bst2UI CCWGG 2 cut(s) 836, 966
Bst4CI ACNGT 9 cut(s) 247, 862, 984, 1491, 1512, 1923, 2529, 2831, 3688
Bst6I CTCTTC 3 cut(s) 219, 2166, 2193
BstACI GRCGYC 1 cut(s) 1033
BstAFI CTTAAG 3 cut(s) 899, 1687, 3092
BstAUI TGTACA 1 cut(s) 1396
BstC8I GCNNGC 2 cut(s) 3493, 3697
BstDSI CCRYGG 3 cut(s) 1616, 2058, 2536
BstENI CCTNNNNNAGG 1 cut(s) 1434
BstFNI CGCG 2 cut(s) 1649, 2060
BstH2I RGCGCY 1 cut(s) 2355
BstHHI GCGC 1 cut(s) 2354
BstMAI GTCTC 7 cut(s) 159, 410, 488, 1857, 2970, 3158, 4814
BstMWI GCNNNNNNNGC 7 cut(s) 1566, 1879, 2941, 3210, 3219, 3497, 3705
BstNI CCWGG 2 cut(s) 836, 966
BstNSI RCATGY 4 cut(s) 500, 1368, 2227, 2469
BstSCI CCNGG 3 cut(s) 834, 964, 1555
BstSFI CTRYAG 2 cut(s) 2672, 2975
BstSLI GKGCMC 2 cut(s) 1794, 2493
BstUI CGCG 2 cut(s) 1649, 2060
BstV2I GAAGAC 2 cut(s) 67, 2336
BstX2I RGATCY 9 cut(s) 640, 824, 1279, 1963, 2080, 2156, 2791, 3063, 3193
BstYI RGATCY 9 cut(s) 640, 824, 1279, 1963, 2080, 2156, 2791, 3063, 3193
BsuI GTATCC 2 cut(s) 1327, 2543
BsuRI GGCC 4 cut(s) 996, 1191, 1615, 2301
BtgI CCRYGG 3 cut(s) 1616, 2058, 2536
BtgZI GCGATG 1 cut(s) 1345
BtsI GCAGTG 2 cut(s) 2669, 3716
BtsIMutI CAGTG 6 cut(s) 168, 858, 2375, 2653, 2669, 3716
BtuMI TCGCGA 1 cut(s) 1649
Cac8I GCNNGC 2 cut(s) 3493, 3697
CaiI CAGNNNCTG 2 cut(s) 2092, 2680
CfoI GCGC 1 cut(s) 2354
Cfr10I RCCGGY 1 cut(s) 1184
Cfr13I GGNCC 2 cut(s) 994, 2011
Cfr42I CCGCGG 1 cut(s) 2061
CseI GACGC 1 cut(s) 1022
Eam1104I CTCTTC 3 cut(s) 219, 2166, 2193
EarI CTCTTC 3 cut(s) 219, 2166, 2193
Ecl136II GAGCTC 1 cut(s) 794
Eco130I CCWWGG 4 cut(s) 1616, 2439, 2536, 2611
Eco147I AGGCCT 1 cut(s) 2301
Eco24I GRGCYC 1 cut(s) 796
Eco31I GGTCTC 1 cut(s) 4814
Eco32I GATATC 1 cut(s) 2870
Eco47I GGWCC 1 cut(s) 2011
Eco47III AGCGCT 1 cut(s) 2353
Eco53kI GAGCTC 1 cut(s) 794
Eco57I CTGAAG 2 cut(s) 1781, 2652
Eco88I CYCGRG 1 cut(s) 2492
EcoICRI GAGCTC 1 cut(s) 794
EcoNI CCTNNNNNAGG 1 cut(s) 1434
EcoO109I RGGNCCY 1 cut(s) 2011
EcoRI GAATTC 1 cut(s) 1889
EcoRII CCWGG 2 cut(s) 834, 964
EcoRV GATATC 1 cut(s) 2870
EcoT14I CCWWGG 4 cut(s) 1616, 2439, 2536, 2611
EcoT22I ATGCAT 2 cut(s) 1500, 2467
EcoT38I GRGCYC 1 cut(s) 796
ErhI CCWWGG 4 cut(s) 1616, 2439, 2536, 2611
FalI AAGNNNNNCTT 2 cut(s) 1334, 1366
FaqI GGGAC 4 cut(s) 339, 1997, 2840, 3340
FauI CCCGC 1 cut(s) 3218
FauNDI CATATG 1 cut(s) 1109
FbaI TGATCA 1 cut(s) 1467
FriOI GRGCYC 1 cut(s) 796
GlaI GCGC 1 cut(s) 2353
GsuI CTGGAG 1 cut(s) 2069
HaeII RGCGCY 1 cut(s) 2355
HaeIII GGCC 4 cut(s) 996, 1191, 1615, 2301
HapII CCGG 8 cut(s) 341, 489, 1185, 1556, 1659, 2207, 2915, 3278
HgaI GACGC 1 cut(s) 1022
HhaI GCGC 1 cut(s) 2354
Hin1I GRCGYC 1 cut(s) 1033
Hin6I GCGC 1 cut(s) 2352
HinP1I GCGC 1 cut(s) 2352
HincII GTYRAC 7 cut(s) 802, 1007, 1115, 4121, 4397, 4673, 4742
HindII GTYRAC 7 cut(s) 802, 1007, 1115, 4121, 4397, 4673, 4742
HindIII AAGCTT 7 cut(s) 176, 462, 1095, 1690, 3263, 3572, 3637
HpaII CCGG 8 cut(s) 341, 489, 1185, 1556, 1659, 2207, 2915, 3278
HpyCH4III ACNGT 9 cut(s) 247, 862, 984, 1491, 1512, 1923, 2529, 2831, 3688
HpyCH4IV ACGT 3 cut(s) 657, 1564, 1819
HpyF10VI GCNNNNNNNGC 7 cut(s) 1566, 1879, 2941, 3210, 3219, 3497, 3705
HpySE526I ACGT 3 cut(s) 657, 1564, 1819
Hsp92I GRCGYC 1 cut(s) 1033
HspAI GCGC 1 cut(s) 2352
Kpn2I TCCGGA 3 cut(s) 488, 2206, 3277
Ksp22I TGATCA 1 cut(s) 1467
KspI CCGCGG 1 cut(s) 2061
LmnI GCTCC 6 cut(s) 750, 2441, 2455, 2636, 3511, 4756
MaeII ACGT 3 cut(s) 657, 1564, 1819
MaeIII GTNAC 6 cut(s) 241, 499, 734, 1987, 2648, 3306
MbiI CCGCTC 1 cut(s) 753
MflI RGATCY 9 cut(s) 640, 824, 1279, 1963, 2080, 2156, 2791, 3063, 3193
MhlI GDGCHC 4 cut(s) 796, 1794, 2405, 2493
MmeI TCCRAC 7 cut(s) 606, 630, 844, 1995, 3181, 3708, 4734
Mph1103I ATGCAT 2 cut(s) 1500, 2467
MroI TCCGGA 3 cut(s) 488, 2206, 3277
MroXI GAANNNNTTC 4 cut(s) 177, 336, 2202, 2751
MslI CAYNNNNRTG 4 cut(s) 1225, 1392, 3221, 3321
MspA1I CMGCKG 4 cut(s) 1145, 1882, 1908, 2060
MspCI CTTAAG 3 cut(s) 899, 1687, 3092
MspI CCGG 8 cut(s) 341, 489, 1185, 1556, 1659, 2207, 2915, 3278
MspR9I CCNGG 3 cut(s) 836, 966, 1557
Mva1269I GAATGC 2 cut(s) 778, 1953
MvaI CCWGG 2 cut(s) 836, 966
MvnI CGCG 2 cut(s) 1649, 2060
MwoI GCNNNNNNNGC 7 cut(s) 1566, 1879, 2941, 3210, 3219, 3497, 3705
NciI CCSGG 1 cut(s) 1557
NcoI CCATGG 2 cut(s) 1616, 2536
NdeI CATATG 1 cut(s) 1109
NheI GCTAGC 1 cut(s) 3695
NlaIV GGNNCC 8 cut(s) 6, 826, 995, 2013, 2257, 2274, 2437, 3084
NmeAIII GCCGAG 1 cut(s) 1022
NmuCI GTSAC 4 cut(s) 499, 1987, 2648, 3306
NruI TCGCGA 1 cut(s) 1649
NsiI ATGCAT 2 cut(s) 1500, 2467
NspI RCATGY 4 cut(s) 500, 1368, 2227, 2469
OliI CACNNNNGTG 1 cut(s) 1225
PceI AGGCCT 1 cut(s) 2301
PctI GAATGC 2 cut(s) 778, 1953
PdmI GAANNNNTTC 4 cut(s) 177, 336, 2202, 2751
PfeI GAWTC 6 cut(s) 150, 518, 730, 1541, 2515, 3340
PflMI CCANNNNNTGG 1 cut(s) 3146
PfoI TCCNGGA 1 cut(s) 834
PpuMI RGGWCCY 1 cut(s) 2011
PshBI ATTAAT 1 cut(s) 3285
PsiI TTATAA 1 cut(s) 1770
Psp124BI GAGCTC 1 cut(s) 796
Psp5II RGGWCCY 1 cut(s) 2011
Psp6I CCWGG 2 cut(s) 834, 964
PspGI CCWGG 2 cut(s) 834, 964
PspN4I GGNNCC 8 cut(s) 6, 826, 995, 2013, 2257, 2274, 2437, 3084
PspPI GGNCC 2 cut(s) 994, 2011
PspPPI RGGWCCY 1 cut(s) 2011
PstI CTGCAG 2 cut(s) 2676, 2979
PstNI CAGNNNCTG 2 cut(s) 2092, 2680
PsuI RGATCY 9 cut(s) 640, 824, 1279, 1963, 2080, 2156, 2791, 3063, 3193
PvuII CAGCTG 3 cut(s) 1145, 1882, 1908
RruI TCGCGA 1 cut(s) 1649
RseI CAYNNNNRTG 4 cut(s) 1225, 1392, 3221, 3321
SacI GAGCTC 1 cut(s) 796
SacII CCGCGG 1 cut(s) 2061
SalI GTCGAC 5 cut(s) 1005, 4119, 4395, 4671, 4740
Sau96I GGNCC 2 cut(s) 994, 2011
ScaI AGTACT 1 cut(s) 2893
ScrFI CCNGG 3 cut(s) 836, 966, 1557
SduI GDGCHC 4 cut(s) 796, 1794, 2405, 2493
SfcI CTRYAG 2 cut(s) 2672, 2975
Sfr303I CCGCGG 1 cut(s) 2061
SgrBI CCGCGG 1 cut(s) 2061
SinI GGWCC 1 cut(s) 2011
SmiMI CAYNNNNRTG 4 cut(s) 1225, 1392, 3221, 3321
SmlI CTYRAG 6 cut(s) 899, 1687, 2021, 2152, 2510, 3092
SmoI CTYRAG 6 cut(s) 899, 1687, 2021, 2152, 2510, 3092
SseBI AGGCCT 1 cut(s) 2301
SsiI CCGC 6 cut(s) 239, 753, 2058, 2060, 2259, 3211
SspI AATATT 1 cut(s) 2940
SstI GAGCTC 1 cut(s) 796
StuI AGGCCT 1 cut(s) 2301
StyD4I CCNGG 3 cut(s) 834, 964, 1555
StyI CCWWGG 4 cut(s) 1616, 2439, 2536, 2611
TaaI ACNGT 9 cut(s) 247, 862, 984, 1491, 1512, 1923, 2529, 2831, 3688
TaiI ACGT 3 cut(s) 660, 1567, 1822
TaqII GACCGA 1 cut(s) 4796
TatI WGTACW 5 cut(s) 1054, 1396, 1599, 1642, 2891
TauI GCSGC 1 cut(s) 756
TfiI GAWTC 6 cut(s) 150, 518, 730, 1541, 2515, 3340
TscAI CASTG 6 cut(s) 175, 865, 2382, 2653, 2676, 3723
TseFI GTSAC 4 cut(s) 499, 1987, 2648, 3306
Tsp45I GTSAC 4 cut(s) 499, 1987, 2648, 3306
TspGWI ACGGA 1 cut(s) 3676
TspRI CASTG 6 cut(s) 175, 865, 2382, 2653, 2676, 3723
Van91I CCANNNNNTGG 1 cut(s) 3146
Vha464I CTTAAG 3 cut(s) 899, 1687, 3092
VpaK11BI GGWCC 1 cut(s) 2011
VspI ATTAAT 1 cut(s) 3285
XagI CCTNNNNNAGG 1 cut(s) 1434
XapI RAATTY 9 cut(s) 1777, 1889, 2104, 2381, 2748, 3746, 3799, 3868, 4006
XbaI TCTAGA 3 cut(s) 922, 2560, 3196
XceI RCATGY 4 cut(s) 500, 1368, 2227, 2469
XcmI CCANNNNNNNNNTGG 1 cut(s) 2543
XmaJI CCTAGG 1 cut(s) 2611
XmnI GAANNNNTTC 4 cut(s) 177, 336, 2202, 2751
ZrmI AGTACT 1 cut(s) 2893
Zsp2I ATGCAT 2 cut(s) 1500, 2467
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.