FvH4_2g08961
RLK Family

Cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000146
Physical Location & Seq
Forward (+)
7883641 .. 7884459
819 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g08961.t1

Sequence Viewer

Length: 819 bp
ATGGTTCTCGATGAAGTACAGATCAGTTCATCTCAAACATCAGCAATCAACTCTGAATCTTCTTCTCAACATAAAGGGCGATCGTCATCTAATGATCATGGCCCTATTGTTCCAGAAAAATTGGATAGCACCAATTTTATTTCTCGGTCTAAACATGTCAAGTTGAATATCACTGGCCGCGGTAAACTTGGTTATCTCATCGGCAAGAAGAAAGCACCATCCGAAGAAGAAGATGAAAAGGGTTACGAAGTTTGGGTTGAAGAAGACAGTCTCGTTCAAGCATGGCTTCTCAGTTCCATGCACAAGGAGGTCAGAGAAAATTATGAAGGTTTGGAGACAGCTAAAGAAATTTGGGATGCTGTTAAAACTACTTTTTCAGTAACCCAAGATGATACACGAAACTATGAGCTACAAATAGCTTCGGTTTCCACCAAACAAAATGGAGCTCCTCTTCATGGCTACTACAACAACATGAAGAAAATATGGCAAGAGCTTGACGTATTGGATCCTCTCAGACTCAAGGATGCAGATTCTATCACCTACATGGCTAATAGGGTCACCAAGCACCGTGTCTATTTCTTTTTGGCCGGGCTAGACCCTCACCTTGATGGAGTCCGGAGTAGAATTCTAAACACAAAGCCGTTGTCTTCTATAGAAGAGGCTTATTCTCAAGTCAGTGCTGAGCATAATAGACTCCAAAAGATGATGTCTGACACAAAGCATGAGGGATCAGCCATGATTGTTCAATACCGGCCACCACATATTCGTAGCTCCCAGCCTTCTTCATCTACACCTCCGTTGAAATCACCCTCTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

273

Amino Acids

30.71

Weight (kDa)

6.56

Isoelectric Point (pI)

57.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotran_gag_2 PF14223 63 - 183 5.9e-09 gag-polypeptide of LTR copia-type
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000146)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19561 FvH4_1g25371 FvH4_1g25961 FvH4_2g00352 FvH4_2g00861 FvH4_2g04952 FvH4_2g05413 FvH4_2g07531 FvH4_2g08961 FvH4_2g25141 FvH4_2g27292 FvH4_3g18164 FvH4_3g19921 FvH4_3g43870 FvH4_4g14441 FvH4_4g15581 FvH4_4g15582 FvH4_4g16890 FvH4_4g19782 FvH4_4g33444 FvH4_4g33921 FvH4_5g22281 FvH4_5g22282 FvH4_5g32571 FvH4_5g37451 FvH4_5g39631 FvH4_6g04721 FvH4_6g11661 FvH4_6g20571 FvH4_6g32791 FvH4_7g23184
prunus_persica Prupe.1G090900_v2.0.a1 Prupe.1G241200_v2.0.a1 Prupe.1G534100_v2.0.a1 Prupe.3G076700_v2.0.a1 Prupe.4G150800_v2.0.a1 Prupe.4G181800_v2.0.a1 Prupe.4G240900_v2.0.a1 Prupe.4G247600_v2.0.a1 Prupe.6G115900_v2.0.a1 Prupe.6G327500_v2.0.a1 Prupe.8G071700_v2.0.a1
pyrus_communis pycom06g11480 pycom10g08330 pycom15g04320 pycom15g04350 pycom15g12620 pycom2874g00060
rosa_chinensis RchiOBHm_Chr1g0317241 RchiOBHm_Chr1g0341801 RchiOBHm_Chr1g0341931 RchiOBHm_Chr1g0359621 RchiOBHm_Chr2g0113741 RchiOBHm_Chr2g0117261 RchiOBHm_Chr2g0138831 RchiOBHm_Chr2g0141421 RchiOBHm_Chr2g0145281 RchiOBHm_Chr2g0152991 RchiOBHm_Chr3g0448001 RchiOBHm_Chr3g0450981 RchiOBHm_Chr3g0455161 RchiOBHm_Chr3g0457401 RchiOBHm_Chr3g0460001 RchiOBHm_Chr4g0387431 RchiOBHm_Chr4g0388741 RchiOBHm_Chr4g0444201 RchiOBHm_Chr4g0444261 RchiOBHm_Chr5g0010221 RchiOBHm_Chr6g0271721 RchiOBHm_Chr6g0274641 RchiOBHm_Chr6g0294691 RchiOBHm_Chr7g0237031 RchiOBHm_Chr7g0238461 RchiOBHm_Chr7g0239981
rosa_laevigata RLG00000009017
rosa_multiflora Rmu_co7986768.1_g000001 Rmu_co8302239.1_g000001 Rmu_co8485799.1_g000001 Rmu_sc0000061.1_g000013 Rmu_sc0000303.1_g000034 Rmu_sc0000303.1_g000035 Rmu_sc0000315.1_g000017 Rmu_sc0000349.1_g000003 Rmu_sc0000498.1_g000068 Rmu_sc0000508.1_g000014 Rmu_sc0000602.1_g000001 Rmu_sc0000633.1_g000002 Rmu_sc0000638.1_g000030 Rmu_sc0000756.1_g000016 Rmu_sc0000756.1_g000017 Rmu_sc0000785.1_g000037 Rmu_sc0000795.1_g000017 Rmu_sc0000796.1_g000030 Rmu_sc0000863.1_g000023 Rmu_sc0000920.1_g000021 Rmu_sc0000960.1_g000015 Rmu_sc0000982.1_g000102 Rmu_sc0001036.1_g000008 Rmu_sc0001036.1_g000009 Rmu_sc0001643.1_g000029 Rmu_sc0001687.1_g000026 Rmu_sc0001708.1_g000037 Rmu_sc0001768.1_g000015 Rmu_sc0001790.1_g000022 Rmu_sc0002180.1_g000007 Rmu_sc0002211.1_g000006 Rmu_sc0002270.1_g000010 Rmu_sc0002404.1_g000031 Rmu_sc0002858.1_g000016 Rmu_sc0003063.1_g000028 Rmu_sc0003730.1_g000017 Rmu_sc0004087.1_g000020 Rmu_sc0004618.1_g000048 Rmu_sc0004724.1_g000005 Rmu_sc0005167.1_g000036 Rmu_sc0005781.1_g000003 Rmu_sc0005878.1_g000012 Rmu_sc0006011.1_g000003 Rmu_sc0006343.1_g000011 Rmu_sc0006573.1_g000007 Rmu_sc0006599.1_g000007 Rmu_sc0006803.1_g000014 Rmu_sc0006836.1_g000002 Rmu_sc0006849.1_g000002 Rmu_sc0007109.1_g000009 Rmu_sc0009268.1_g000016 Rmu_sc0009618.1_g000017 Rmu_sc0009674.1_g000009 Rmu_sc0010244.1_g000009 Rmu_sc0011151.1_g000004 Rmu_sc0011356.1_g000001 Rmu_sc0011674.1_g000002 Rmu_sc0011778.1_g000016 Rmu_sc0012342.1_g000001 Rmu_sc0012467.1_g000002 Rmu_sc0012574.1_g000003 Rmu_sc0012913.1_g000005 Rmu_sc0013612.1_g000020 Rmu_sc0014824.1_g000001 Rmu_sc0017869.1_g000002 Rmu_sc0018085.1_g000003 Rmu_sc0024789.1_g000001 Rmu_sc0025106.1_g000013 Rmu_sc0034502.1_g000001 Rmu_sc0034547.1_g000001 Rmu_sc0035094.1_g000001 Rmu_sc0035095.1_g000001 Rmu_ssc0000100.1_g000027 Rmu_ssc0000244.1_g000005 Rmu_ssc0000371.1_g000029 Rmu_ssc0000381.1_g000004
rosa_roxburghii Rroxscaffold_1G00015480 Rroxscaffold_1G00023580 Rroxscaffold_1G00039340 Rroxscaffold_1G00047080 Rroxscaffold_2G00095820 Rroxscaffold_2G00125200 Rroxscaffold_2G00128330 Rroxscaffold_3G00270860
rosa_rugosa Rorug06G0120700
rosa_samantha Rh3BG173100 Rh6AG089600 Rh7AG488100 Rh7DG389600
rosa_wichuraiana Rw0G001150 Rw0G001990 Rw0G002010 Rw0G019540 Rw1G013730 Rw1G024110 Rw1G029190 Rw2G018850 Rw2G021090 Rw2G022260 Rw2G023180 Rw2G027280 Rw3G010190 Rw3G020660 Rw3G029380 Rw4G002390 Rw4G002690 Rw4G033260 Rw5G011960 Rw5G023110 Rw5G025250 Rw5G028950 Rw5G033740 Rw5G033950 Rw5G037900 Rw6G009640 Rw6G011620 Rw6G027720 Rw7G010680 Rw7G042070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 180
AccIII TCCGGA 1 cut(s) 615
AciI CCGC 2 cut(s) 178, 180
AclWI GGATC 3 cut(s) 500, 513, 736
AcoI YGGCCR 3 cut(s) 175, 585, 752
AcsI RAATTY 2 cut(s) 348, 624
AfaI GTAC 1 cut(s) 18
AfiI CCNNNNNNNGG 1 cut(s) 455
AflIII ACRYGT 1 cut(s) 154
AgsI TTSAA 5 cut(s) 166, 260, 278, 746, 802
AluBI AGCT 6 cut(s) 341, 409, 419, 446, 493, 771
AluI AGCT 6 cut(s) 341, 409, 419, 446, 493, 771
Alw21I GWGCWC 1 cut(s) 448
Alw26I GTCTC 2 cut(s) 275, 329
AlwI GGATC 3 cut(s) 500, 513, 736
Aor13HI TCCGGA 1 cut(s) 615
AoxI GGCC 4 cut(s) 100, 175, 585, 752
ApoI RAATTY 2 cut(s) 348, 624
ArsI GACNNNNNNTTYG 2 cut(s) 629, 661
AspS9I GGNCC 1 cut(s) 101
AsuC2I CCSGG 1 cut(s) 589
AsuHPI GGTGA 4 cut(s) 529, 550, 593, 798
BamHI GGATCC 1 cut(s) 505
BanII GRGCYC 1 cut(s) 448
BbsI GAAGAC 2 cut(s) 270, 639
Bbv12I GWGCWC 1 cut(s) 448
BccI CCATC 2 cut(s) 226, 602
BceAI ACGGC 1 cut(s) 625
BclI TGATCA 1 cut(s) 94
BcnI CCSGG 1 cut(s) 589
BcoDI GTCTC 2 cut(s) 275, 329
BfaI CTAG 1 cut(s) 593
BfmI CTRYAG 1 cut(s) 651
BisI GCNGC 1 cut(s) 178
BlpI GCTNAGC 1 cut(s) 681
BlsI GCNGC 1 cut(s) 179
Bme1390I CCNGG 1 cut(s) 589
BmgT120I GGNCC 1 cut(s) 101
BmiI GGNNCC 1 cut(s) 507
BmrFI CCNGG 1 cut(s) 589
BmsI GCATC 2 cut(s) 346, 514
BpiI GAAGAC 2 cut(s) 270, 639
Bpu1102I GCTNAGC 1 cut(s) 681
BpuEI CTTGAG 2 cut(s) 503, 654
BpuMI CCSGG 1 cut(s) 589
BsaBI GATNNNNATC 1 cut(s) 85
BsaJI CCNNGG 1 cut(s) 178
BsaWI WCCGGW 1 cut(s) 615
Bsc4I CCNNNNNNNGG 1 cut(s) 455
Bse118I RCCGGY 1 cut(s) 750
Bse1I ACTGG 1 cut(s) 178
Bse8I GATNNNNATC 1 cut(s) 85
BseAI TCCGGA 1 cut(s) 615
BseDI CCNNGG 1 cut(s) 178
BseGI GGATG 3 cut(s) 218, 361, 529
BseJI GATNNNNATC 1 cut(s) 85
BseLI CCNNNNNNNGG 1 cut(s) 455
BseMII CTCAG 3 cut(s) 304, 526, 672
BseNI ACTGG 1 cut(s) 178
BseRI GAGGAG 1 cut(s) 438
BseYI CCCAGC 1 cut(s) 774
Bsh1236I CGCG 1 cut(s) 180
Bsh1285I CGRYCG 1 cut(s) 83
BshFI GGCC 4 cut(s) 102, 177, 587, 754
BsiEI CGRYCG 1 cut(s) 83
BsiHKAI GWGCWC 1 cut(s) 448
BsiSI CCGG 3 cut(s) 588, 616, 751
BslI CCNNNNNNNGG 1 cut(s) 455
BsmAI GTCTC 2 cut(s) 275, 329
BsnI GGCC 4 cut(s) 102, 177, 587, 754
Bsp1286I GDGCHC 1 cut(s) 448
Bsp13I TCCGGA 1 cut(s) 615
Bsp143I GATC 5 cut(s) 21, 80, 94, 505, 728
Bsp1720I GCTNAGC 1 cut(s) 681
BspACI CCGC 2 cut(s) 178, 180
BspANI GGCC 4 cut(s) 102, 177, 587, 754
BspCNI CTCAG 3 cut(s) 303, 525, 673
BspEI TCCGGA 1 cut(s) 615
BspFNI CGCG 1 cut(s) 180
BspLI GGNNCC 1 cut(s) 507
BspPI GGATC 3 cut(s) 500, 513, 736
BsrFI RCCGGY 1 cut(s) 750
BsrI ACTGG 1 cut(s) 178
BssAI RCCGGY 1 cut(s) 750
BssECI CCNNGG 1 cut(s) 178
BssMI GATC 5 cut(s) 21, 80, 94, 505, 728
Bst4CI ACNGT 2 cut(s) 269, 569
Bst6I CTCTTC 2 cut(s) 456, 651
BstDEI CTNAG 3 cut(s) 290, 512, 681
BstDSI CCRYGG 1 cut(s) 178
BstEII GGTNACC 1 cut(s) 556
BstF5I GGATG 3 cut(s) 218, 361, 529
BstFNI CGCG 1 cut(s) 180
BstKTI GATC 5 cut(s) 24, 83, 97, 508, 731
BstMAI GTCTC 2 cut(s) 275, 329
BstMBI GATC 5 cut(s) 21, 80, 94, 505, 728
BstMCI CGRYCG 1 cut(s) 83
BstNSI RCATGY 1 cut(s) 158
BstPI GGTNACC 1 cut(s) 556
BstSCI CCNGG 1 cut(s) 587
BstSFI CTRYAG 1 cut(s) 651
BstUI CGCG 1 cut(s) 180
BstV2I GAAGAC 2 cut(s) 270, 639
BstX2I RGATCY 1 cut(s) 505
BstYI RGATCY 1 cut(s) 505
BsuRI GGCC 4 cut(s) 102, 177, 587, 754
BtgI CCRYGG 1 cut(s) 178
BtsCI GGATG 3 cut(s) 218, 361, 529
BtsIMutI CAGTG 2 cut(s) 171, 682
Cfr10I RCCGGY 1 cut(s) 750
Cfr13I GGNCC 1 cut(s) 101
Cfr42I CCGCGG 1 cut(s) 181
Csp6I GTAC 1 cut(s) 17
CviAII CATG 9 cut(s) 98, 155, 282, 298, 455, 472, 544, 722, 736
CviQI GTAC 1 cut(s) 17
DdeI CTNAG 3 cut(s) 290, 512, 681
DpnI GATC 5 cut(s) 23, 82, 96, 507, 730
DpnII GATC 5 cut(s) 21, 80, 94, 505, 728
EaeI YGGCCR 3 cut(s) 175, 585, 752
Eam1104I CTCTTC 2 cut(s) 456, 651
EarI CTCTTC 2 cut(s) 456, 651
Ecl136II GAGCTC 1 cut(s) 446
Eco24I GRGCYC 1 cut(s) 448
Eco53kI GAGCTC 1 cut(s) 446
Eco91I GGTNACC 1 cut(s) 556
EcoICRI GAGCTC 1 cut(s) 446
EcoO65I GGTNACC 1 cut(s) 556
EcoRI GAATTC 1 cut(s) 624
EcoT38I GRGCYC 1 cut(s) 448
FaeI CATG 9 cut(s) 101, 158, 285, 301, 458, 475, 547, 725, 739
FalI AAGNNNNNCTT 2 cut(s) 270, 302
FatI CATG 9 cut(s) 97, 154, 281, 297, 454, 471, 543, 721, 735
FbaI TGATCA 1 cut(s) 94
Fnu4HI GCNGC 1 cut(s) 178
FokI GGATG 3 cut(s) 205, 368, 536
FriOI GRGCYC 1 cut(s) 448
Fsp4HI GCNGC 1 cut(s) 178
FspBI CTAG 1 cut(s) 593
GluI GCNGC 1 cut(s) 178
GsaI CCCAGC 1 cut(s) 778
HaeIII GGCC 4 cut(s) 102, 177, 587, 754
HapII CCGG 3 cut(s) 588, 616, 751
Hin1II CATG 9 cut(s) 101, 158, 285, 301, 458, 475, 547, 725, 739
HinfI GANTC 5 cut(s) 56, 516, 530, 612, 693
HpaII CCGG 3 cut(s) 588, 616, 751
HphI GGTGA 4 cut(s) 529, 550, 593, 798
Hpy166II GTNNAC 1 cut(s) 185
Hpy188I TCNGA 5 cut(s) 55, 223, 314, 515, 712
Hpy188III TCNNGA 3 cut(s) 8, 113, 616
Hpy8I GTNNAC 1 cut(s) 185
HpyAV CCTTC 2 cut(s) 320, 789
HpyCH4III ACNGT 2 cut(s) 269, 569
HpyCH4IV ACGT 1 cut(s) 498
HpyCH4V TGCA 2 cut(s) 301, 527
HpyF3I CTNAG 3 cut(s) 290, 512, 681
HpySE526I ACGT 1 cut(s) 498
Hsp92II CATG 9 cut(s) 101, 158, 285, 301, 458, 475, 547, 725, 739
Kpn2I TCCGGA 1 cut(s) 615
Ksp22I TGATCA 1 cut(s) 94
KspI CCGCGG 1 cut(s) 181
Kzo9I GATC 5 cut(s) 21, 80, 94, 505, 728
LmnI GCTCC 3 cut(s) 443, 451, 776
LpnPI CCDG 6 cut(s) 126, 159, 601, 629, 764, 788
LweI GCATC 2 cut(s) 346, 514
MaeI CTAG 1 cut(s) 593
MaeII ACGT 1 cut(s) 498
MaeIII GTNAC 3 cut(s) 242, 379, 556
MalI GATC 5 cut(s) 23, 82, 96, 507, 730
MboI GATC 5 cut(s) 21, 80, 94, 505, 728
MflI RGATCY 1 cut(s) 505
MhlI GDGCHC 1 cut(s) 448
MluCI AATT 5 cut(s) 119, 133, 319, 348, 624
MlyI GAGTC 3 cut(s) 510, 621, 687
MnlI CCTC 7 cut(s) 301, 459, 519, 609, 652, 718, 804
MroI TCCGGA 1 cut(s) 615
MseI TTAA 1 cut(s) 363
MslI CAYNNNNRTG 2 cut(s) 542, 606
MspA1I CMGCKG 1 cut(s) 180
MspI CCGG 3 cut(s) 588, 616, 751
MspR9I CCNGG 1 cut(s) 589
MvnI CGCG 1 cut(s) 180
NciI CCSGG 1 cut(s) 589
NdeII GATC 5 cut(s) 21, 80, 94, 505, 728
NlaIII CATG 9 cut(s) 101, 158, 285, 301, 458, 475, 547, 725, 739
NlaIV GGNNCC 1 cut(s) 507
NmuCI GTSAC 1 cut(s) 556
NspI RCATGY 1 cut(s) 158
PciI ACATGT 1 cut(s) 154
PfeI GAWTC 2 cut(s) 56, 530
PkrI GCNGC 1 cut(s) 179
Ple19I CGATCG 1 cut(s) 83
PleI GAGTC 3 cut(s) 510, 620, 687
PpsI GAGTC 3 cut(s) 510, 620, 687
PscI ACATGT 1 cut(s) 154
Psp124BI GAGCTC 1 cut(s) 448
PspEI GGTNACC 1 cut(s) 556
PspFI CCCAGC 1 cut(s) 774
PspN4I GGNNCC 1 cut(s) 507
PspPI GGNCC 1 cut(s) 101
PsuI RGATCY 1 cut(s) 505
PvuI CGATCG 1 cut(s) 83
RsaI GTAC 1 cut(s) 18
RsaNI GTAC 1 cut(s) 17
RseI CAYNNNNRTG 2 cut(s) 542, 606
SacI GAGCTC 1 cut(s) 448
SacII CCGCGG 1 cut(s) 181
SaqAI TTAA 1 cut(s) 363
SatI GCNGC 1 cut(s) 178
Sau3AI GATC 5 cut(s) 21, 80, 94, 505, 728
Sau96I GGNCC 1 cut(s) 101
SchI GAGTC 3 cut(s) 510, 621, 687
ScrFI CCNGG 1 cut(s) 589
SduI GDGCHC 1 cut(s) 448
SfaNI GCATC 2 cut(s) 346, 514
SfcI CTRYAG 1 cut(s) 651
Sfr303I CCGCGG 1 cut(s) 181
SgrBI CCGCGG 1 cut(s) 181
SmiMI CAYNNNNRTG 2 cut(s) 542, 606
SmlI CTYRAG 2 cut(s) 518, 669
SmoI CTYRAG 2 cut(s) 518, 669
Sse9I AATT 5 cut(s) 119, 133, 319, 348, 624
SsiI CCGC 2 cut(s) 178, 180
SspMI CTAG 1 cut(s) 593
SstI GAGCTC 1 cut(s) 448
StyD4I CCNGG 1 cut(s) 587
TaaI ACNGT 2 cut(s) 269, 569
TaiI ACGT 1 cut(s) 501
TaqI TCGA 1 cut(s) 9
TaqII GACCGA 1 cut(s) 135
TasI AATT 5 cut(s) 119, 133, 319, 348, 624
TatI WGTACW 1 cut(s) 16
TauI GCSGC 1 cut(s) 180
TfiI GAWTC 2 cut(s) 56, 530
Tru1I TTAA 1 cut(s) 363
Tru9I TTAA 1 cut(s) 363
TscAI CASTG 2 cut(s) 178, 682
TseFI GTSAC 1 cut(s) 556
Tsp45I GTSAC 1 cut(s) 556
TspDTI ATGAA 7 cut(s) 18, 27, 249, 339, 443, 488, 774
TspGWI ACGGA 1 cut(s) 786
TspRI CASTG 2 cut(s) 178, 682
XapI RAATTY 2 cut(s) 348, 624
XceI RCATGY 1 cut(s) 158
XspI CTAG 1 cut(s) 593
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.