Rw3G029380
RLK Family

Cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000146
Physical Location & Seq
Reverse (-)
45252763 .. 45256731
3969 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G029380.1

Sequence Viewer

Length: 984 bp
ATGCGTGTCCATATTTTTCTTGCTGGCCTGGACCCTCACTTTGAAGGGGCAAAGAATGAGTTATTGCGTCTTGCAACTCCACCTACATTGGAACAAGCTTTTGCCTATATCCGAAGAGATGAAGGTAATAAGGCTGCCGCTCAGAACCTTCATACTGAAATTTCTGGTTTAGCAATCCATGCTACACCTCCACCTCCACCTCAACCTCAGATTGGGAATTCTACCCTAGTTTCATCTCAGAGTCAGTATCAGCCACGGAATCAGGGATATCAGCAGAACCAGAATTATAATCAAATGTCTTGCAACTATTGCAAGGAAGTTGGCCATTTCAAGAATCAATGTCCTAAGCTGCGAAGGTTTAATTACAACAACTCTGGTTGGAGAGGAGGCAATAATACTGGAGGACGTGAGGACAATCTGTGTACCAGGGTGATCATGGGCAAGGGAGACCTGAGGGGGAGACTGTTTCACTTGGATTGCATGTACGCAGAGCCAACACAAGCACCAGAACAGCCTTTAGCCCTGACATTGAATTCTGACAGATTAAGTGAGCTATGGTTGTGGCATAGGTGTTTGGGTCATCCATCTTTTGGAGTTATGAAGAAGTCCATGCCTTCATTGTTTCTGGGAGTTAGTGAGTCTAGCCTGCATTGTGAAACTTGTGCTTTGGCTAAGAGTCATAGGTCTAGTTATCCTTCGAGTTTTCATTCTAGTACTATGCCTTTTGAGTTAATTCATTCAGATGTATGGGGTCCTTCTAAACATTCTACCCTTTCTGGAATGCGATATTTTCATGATCGCACGAAGCATGTGGAGGTAGATCGACACTTCATTAAAGAGAAGCTGGATGGACAGATCATTATGTTTCCCTTCGTTCCTACTGAAGAACAGTTGGCGGATATTCTTACAAAGGCTCTCTCGAGTAAAGCCTTCTATGACTCACTTGACAAGTTGGGCATCCGTGATCTGTATGCACCAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Retrotran_gag_2

Protein Analysis

327

Amino Acids

36.99

Weight (kDa)

8.56

Isoelectric Point (pI)

53.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
gag_pre-integrs PF13976 176 - 226 2.5e-13 GAG-pre-integrase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000146)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19561 FvH4_1g25371 FvH4_1g25961 FvH4_2g00352 FvH4_2g00861 FvH4_2g04952 FvH4_2g05413 FvH4_2g07531 FvH4_2g08961 FvH4_2g25141 FvH4_2g27292 FvH4_3g18164 FvH4_3g19921 FvH4_3g43870 FvH4_4g14441 FvH4_4g15581 FvH4_4g15582 FvH4_4g16890 FvH4_4g19782 FvH4_4g33444 FvH4_4g33921 FvH4_5g22281 FvH4_5g22282 FvH4_5g32571 FvH4_5g37451 FvH4_5g39631 FvH4_6g04721 FvH4_6g11661 FvH4_6g20571 FvH4_6g32791 FvH4_7g23184
prunus_persica Prupe.1G090900_v2.0.a1 Prupe.1G241200_v2.0.a1 Prupe.1G534100_v2.0.a1 Prupe.3G076700_v2.0.a1 Prupe.4G150800_v2.0.a1 Prupe.4G181800_v2.0.a1 Prupe.4G240900_v2.0.a1 Prupe.4G247600_v2.0.a1 Prupe.6G115900_v2.0.a1 Prupe.6G327500_v2.0.a1 Prupe.8G071700_v2.0.a1
pyrus_communis pycom06g11480 pycom10g08330 pycom15g04320 pycom15g04350 pycom15g12620 pycom2874g00060
rosa_chinensis RchiOBHm_Chr1g0317241 RchiOBHm_Chr1g0341801 RchiOBHm_Chr1g0341931 RchiOBHm_Chr1g0359621 RchiOBHm_Chr2g0113741 RchiOBHm_Chr2g0117261 RchiOBHm_Chr2g0138831 RchiOBHm_Chr2g0141421 RchiOBHm_Chr2g0145281 RchiOBHm_Chr2g0152991 RchiOBHm_Chr3g0448001 RchiOBHm_Chr3g0450981 RchiOBHm_Chr3g0455161 RchiOBHm_Chr3g0457401 RchiOBHm_Chr3g0460001 RchiOBHm_Chr4g0387431 RchiOBHm_Chr4g0388741 RchiOBHm_Chr4g0444201 RchiOBHm_Chr4g0444261 RchiOBHm_Chr5g0010221 RchiOBHm_Chr6g0271721 RchiOBHm_Chr6g0274641 RchiOBHm_Chr6g0294691 RchiOBHm_Chr7g0237031 RchiOBHm_Chr7g0238461 RchiOBHm_Chr7g0239981
rosa_laevigata RLG00000009017
rosa_multiflora Rmu_co7986768.1_g000001 Rmu_co8302239.1_g000001 Rmu_co8485799.1_g000001 Rmu_sc0000061.1_g000013 Rmu_sc0000303.1_g000034 Rmu_sc0000303.1_g000035 Rmu_sc0000315.1_g000017 Rmu_sc0000349.1_g000003 Rmu_sc0000498.1_g000068 Rmu_sc0000508.1_g000014 Rmu_sc0000602.1_g000001 Rmu_sc0000633.1_g000002 Rmu_sc0000638.1_g000030 Rmu_sc0000756.1_g000016 Rmu_sc0000756.1_g000017 Rmu_sc0000785.1_g000037 Rmu_sc0000795.1_g000017 Rmu_sc0000796.1_g000030 Rmu_sc0000863.1_g000023 Rmu_sc0000920.1_g000021 Rmu_sc0000960.1_g000015 Rmu_sc0000982.1_g000102 Rmu_sc0001036.1_g000008 Rmu_sc0001036.1_g000009 Rmu_sc0001643.1_g000029 Rmu_sc0001687.1_g000026 Rmu_sc0001708.1_g000037 Rmu_sc0001768.1_g000015 Rmu_sc0001790.1_g000022 Rmu_sc0002180.1_g000007 Rmu_sc0002211.1_g000006 Rmu_sc0002270.1_g000010 Rmu_sc0002404.1_g000031 Rmu_sc0002858.1_g000016 Rmu_sc0003063.1_g000028 Rmu_sc0003730.1_g000017 Rmu_sc0004087.1_g000020 Rmu_sc0004618.1_g000048 Rmu_sc0004724.1_g000005 Rmu_sc0005167.1_g000036 Rmu_sc0005781.1_g000003 Rmu_sc0005878.1_g000012 Rmu_sc0006011.1_g000003 Rmu_sc0006343.1_g000011 Rmu_sc0006573.1_g000007 Rmu_sc0006599.1_g000007 Rmu_sc0006803.1_g000014 Rmu_sc0006836.1_g000002 Rmu_sc0006849.1_g000002 Rmu_sc0007109.1_g000009 Rmu_sc0009268.1_g000016 Rmu_sc0009618.1_g000017 Rmu_sc0009674.1_g000009 Rmu_sc0010244.1_g000009 Rmu_sc0011151.1_g000004 Rmu_sc0011356.1_g000001 Rmu_sc0011674.1_g000002 Rmu_sc0011778.1_g000016 Rmu_sc0012342.1_g000001 Rmu_sc0012467.1_g000002 Rmu_sc0012574.1_g000003 Rmu_sc0012913.1_g000005 Rmu_sc0013612.1_g000020 Rmu_sc0014824.1_g000001 Rmu_sc0017869.1_g000002 Rmu_sc0018085.1_g000003 Rmu_sc0024789.1_g000001 Rmu_sc0025106.1_g000013 Rmu_sc0034502.1_g000001 Rmu_sc0034547.1_g000001 Rmu_sc0035094.1_g000001 Rmu_sc0035095.1_g000001 Rmu_ssc0000100.1_g000027 Rmu_ssc0000244.1_g000005 Rmu_ssc0000371.1_g000029 Rmu_ssc0000381.1_g000004
rosa_roxburghii Rroxscaffold_1G00015480 Rroxscaffold_1G00023580 Rroxscaffold_1G00039340 Rroxscaffold_1G00047080 Rroxscaffold_2G00095820 Rroxscaffold_2G00125200 Rroxscaffold_2G00128330 Rroxscaffold_3G00270860
rosa_rugosa Rorug06G0120700
rosa_samantha Rh3BG173100 Rh6AG089600 Rh7AG488100 Rh7DG389600
rosa_wichuraiana Rw0G001150 Rw0G001990 Rw0G002010 Rw0G019540 Rw1G013730 Rw1G024110 Rw1G029190 Rw2G018850 Rw2G021090 Rw2G022260 Rw2G023180 Rw2G027280 Rw3G010190 Rw3G020660 Rw3G029380 Rw4G002390 Rw4G002690 Rw4G033260 Rw5G011960 Rw5G023110 Rw5G025250 Rw5G028950 Rw5G033740 Rw5G033950 Rw5G037900 Rw6G009640 Rw6G011620 Rw6G027720 Rw7G010680 Rw7G042070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 288
AccB7I CCANNNNNTGG 1 cut(s) 590
AccBSI CCGCTC 1 cut(s) 140
AciI CCGC 2 cut(s) 138, 896
AcoI YGGCCR 1 cut(s) 322
AcsI RAATTY 3 cut(s) 159, 217, 532
AcuI CTGAAG 1 cut(s) 903
AfaI GTAC 3 cut(s) 424, 485, 715
AfiI CCNNNNNNNGG 2 cut(s) 212, 590
AgsI TTSAA 3 cut(s) 44, 331, 532
AjiI CACGTC 1 cut(s) 407
AjnI CCWGG 2 cut(s) 27, 425
AluBI AGCT 4 cut(s) 98, 349, 553, 844
AluI AGCT 4 cut(s) 98, 349, 553, 844
Alw26I GTCTC 2 cut(s) 441, 454
Ama87I CYCGRG 1 cut(s) 919
AoxI GGCC 2 cut(s) 25, 322
ApeKI GCWGC 2 cut(s) 134, 349
ApoI RAATTY 3 cut(s) 159, 217, 532
ArsI GACNNNNNNTTYG 2 cut(s) 23, 55
AspS9I GGNCC 2 cut(s) 31, 752
AsuHPI GGTGA 1 cut(s) 442
AvaI CYCGRG 1 cut(s) 919
AvaII GGWCC 2 cut(s) 31, 752
AxyI CCTNAGG 1 cut(s) 452
BalI TGGCCA 1 cut(s) 324
BbvI GCAGC 2 cut(s) 121, 336
BccI CCATC 2 cut(s) 592, 842
BciT130I CCWGG 2 cut(s) 29, 427
BclI TGATCA 1 cut(s) 432
BcoDI GTCTC 2 cut(s) 441, 454
BfaI CTAG 4 cut(s) 227, 642, 687, 711
BisI GCNGC 3 cut(s) 135, 138, 350
BlsI GCNGC 3 cut(s) 136, 139, 351
BmcAI AGTACT 1 cut(s) 715
Bme1390I CCNGG 2 cut(s) 29, 427
Bme18I GGWCC 2 cut(s) 31, 752
BmeT110I CYCGRG 1 cut(s) 919
BmgBI CACGTC 1 cut(s) 407
BmgT120I GGNCC 2 cut(s) 31, 752
BmiI GGNNCC 2 cut(s) 33, 753
BmrFI CCNGG 2 cut(s) 29, 427
BmsI GCATC 1 cut(s) 966
BpmI CTGGAG 1 cut(s) 420
Bpu10I CCTNAGC 1 cut(s) 345
BsaI GGTCTC 1 cut(s) 441
BsaJI CCNNGG 2 cut(s) 254, 426
Bsc4I CCNNNNNNNGG 2 cut(s) 212, 590
Bse1I ACTGG 1 cut(s) 403
Bse21I CCTNAGG 1 cut(s) 452
BseBI CCWGG 2 cut(s) 29, 427
BseDI CCNNGG 2 cut(s) 254, 426
BseGI GGATG 3 cut(s) 580, 853, 957
BseLI CCNNNNNNNGG 2 cut(s) 212, 590
BseMII CTCAG 4 cut(s) 155, 221, 251, 443
BseNI ACTGG 1 cut(s) 403
BseRI GAGGAG 1 cut(s) 399
BseXI GCAGC 2 cut(s) 121, 336
BshFI GGCC 2 cut(s) 27, 324
BsiHKCI CYCGRG 1 cut(s) 919
BslI CCNNNNNNNGG 2 cut(s) 212, 590
BsmAI GTCTC 2 cut(s) 441, 454
BsmI GAATGC 1 cut(s) 786
BsnI GGCC 2 cut(s) 27, 324
Bso31I GGTCTC 1 cut(s) 441
BsoBI CYCGRG 1 cut(s) 919
Bsp143I GATC 5 cut(s) 432, 796, 820, 855, 964
BspACI CCGC 2 cut(s) 138, 896
BspANI GGCC 2 cut(s) 27, 324
BspCNI CTCAG 4 cut(s) 154, 220, 250, 444
BspHI TCATGA 1 cut(s) 793
BspLI GGNNCC 2 cut(s) 33, 753
BspTNI GGTCTC 1 cut(s) 441
BsrBI CCGCTC 1 cut(s) 140
BsrI ACTGG 1 cut(s) 403
BssECI CCNNGG 2 cut(s) 254, 426
BssMI GATC 5 cut(s) 432, 796, 820, 855, 964
Bst2UI CCWGG 2 cut(s) 29, 427
Bst4CI ACNGT 2 cut(s) 465, 891
Bst6I CTCTTC 1 cut(s) 109
BstAPI GCANNNNNTGC 2 cut(s) 179, 309
BstC8I GCNNGC 2 cut(s) 25, 647
BstDEI CTNAG 6 cut(s) 141, 207, 237, 345, 452, 672
BstDSI CCRYGG 1 cut(s) 254
BstF5I GGATG 3 cut(s) 580, 853, 957
BstKTI GATC 5 cut(s) 435, 799, 823, 858, 967
BstMAI GTCTC 2 cut(s) 441, 454
BstMBI GATC 5 cut(s) 432, 796, 820, 855, 964
BstMWI GCNNNNNNNGC 2 cut(s) 179, 309
BstNI CCWGG 2 cut(s) 29, 427
BstNSI RCATGY 2 cut(s) 484, 812
BstSCI CCNGG 2 cut(s) 27, 425
BstV1I GCAGC 2 cut(s) 121, 336
Bsu36I CCTNAGG 1 cut(s) 452
BsuRI GGCC 2 cut(s) 27, 324
BtgI CCRYGG 1 cut(s) 254
BtrI CACGTC 1 cut(s) 407
BtsCI GGATG 3 cut(s) 580, 853, 957
Cac8I GCNNGC 2 cut(s) 25, 647
CciI TCATGA 1 cut(s) 793
Cfr13I GGNCC 2 cut(s) 31, 752
CseI GACGC 1 cut(s) 56
Csp6I GTAC 3 cut(s) 423, 484, 714
CspCI CAANNNNNGTGG 2 cut(s) 69, 104
CviAII CATG 6 cut(s) 179, 436, 481, 610, 794, 809
CviQI GTAC 3 cut(s) 423, 484, 714
DdeI CTNAG 6 cut(s) 141, 207, 237, 345, 452, 672
DpnI GATC 5 cut(s) 434, 798, 822, 857, 966
DpnII GATC 5 cut(s) 432, 796, 820, 855, 964
EaeI YGGCCR 1 cut(s) 322
Eam1104I CTCTTC 1 cut(s) 109
EarI CTCTTC 1 cut(s) 109
EciI GGCGGA 1 cut(s) 911
Eco31I GGTCTC 1 cut(s) 441
Eco32I GATATC 1 cut(s) 269
Eco47I GGWCC 2 cut(s) 31, 752
Eco57I CTGAAG 1 cut(s) 903
Eco81I CCTNAGG 1 cut(s) 452
Eco88I CYCGRG 1 cut(s) 919
EcoO109I RGGNCCY 1 cut(s) 752
EcoRI GAATTC 2 cut(s) 217, 532
EcoRII CCWGG 2 cut(s) 27, 425
EcoRV GATATC 1 cut(s) 269
FaeI CATG 6 cut(s) 182, 439, 484, 613, 797, 812
FatI CATG 6 cut(s) 178, 435, 480, 609, 793, 808
FbaI TGATCA 1 cut(s) 432
Fnu4HI GCNGC 3 cut(s) 135, 138, 350
FokI GGATG 3 cut(s) 567, 860, 944
Fsp4HI GCNGC 3 cut(s) 135, 138, 350
FspBI CTAG 4 cut(s) 227, 642, 687, 711
GluI GCNGC 3 cut(s) 135, 138, 350
GsuI CTGGAG 1 cut(s) 420
HaeIII GGCC 2 cut(s) 27, 324
HgaI GACGC 1 cut(s) 56
Hin1II CATG 6 cut(s) 182, 439, 484, 613, 797, 812
HindIII AAGCTT 1 cut(s) 96
HinfI GANTC 6 cut(s) 241, 259, 334, 638, 676, 938
HphI GGTGA 1 cut(s) 442
Hpy166II GTNNAC 1 cut(s) 423
Hpy188I TCNGA 6 cut(s) 113, 144, 210, 240, 538, 742
Hpy188III TCNNGA 4 cut(s) 331, 777, 794, 919
Hpy8I GTNNAC 1 cut(s) 423
HpyAV CCTTC 9 cut(s) 38, 116, 158, 348, 624, 705, 765, 880, 940
HpyCH4III ACNGT 2 cut(s) 465, 891
HpyCH4IV ACGT 1 cut(s) 406
HpyCH4V TGCA 6 cut(s) 74, 303, 312, 480, 649, 974
HpyF10VI GCNNNNNNNGC 2 cut(s) 179, 309
HpyF3I CTNAG 6 cut(s) 141, 207, 237, 345, 452, 672
HpySE526I ACGT 1 cut(s) 406
Hsp92II CATG 6 cut(s) 182, 439, 484, 613, 797, 812
Ksp22I TGATCA 1 cut(s) 432
Kzo9I GATC 5 cut(s) 432, 796, 820, 855, 964
Lsp1109I GCAGC 2 cut(s) 121, 336
LweI GCATC 1 cut(s) 966
MaeI CTAG 4 cut(s) 227, 642, 687, 711
MaeII ACGT 1 cut(s) 406
MalI GATC 5 cut(s) 434, 798, 822, 857, 966
MbiI CCGCTC 1 cut(s) 140
MboI GATC 5 cut(s) 432, 796, 820, 855, 964
MboII GAAGA 3 cut(s) 126, 613, 896
MlsI TGGCCA 1 cut(s) 324
MluCI AATT 6 cut(s) 159, 217, 283, 361, 532, 732
MluNI TGGCCA 1 cut(s) 324
MlyI GAGTC 4 cut(s) 250, 647, 685, 932
MmeI TCCRAC 1 cut(s) 359
Mox20I TGGCCA 1 cut(s) 324
MscI TGGCCA 1 cut(s) 324
MseI TTAA 4 cut(s) 360, 545, 731, 834
MslI CAYNNNNRTG 1 cut(s) 741
Msp20I TGGCCA 1 cut(s) 324
MspR9I CCNGG 2 cut(s) 29, 427
Mva1269I GAATGC 1 cut(s) 786
MvaI CCWGG 2 cut(s) 29, 427
MwoI GCNNNNNNNGC 2 cut(s) 179, 309
NdeII GATC 5 cut(s) 432, 796, 820, 855, 964
NlaIII CATG 6 cut(s) 182, 439, 484, 613, 797, 812
NlaIV GGNNCC 2 cut(s) 33, 753
NspI RCATGY 2 cut(s) 484, 812
PaeR7I CTCGAG 1 cut(s) 919
PagI TCATGA 1 cut(s) 793
PctI GAATGC 1 cut(s) 786
PfeI GAWTC 2 cut(s) 259, 334
PflMI CCANNNNNTGG 1 cut(s) 590
PkrI GCNGC 3 cut(s) 136, 139, 351
PleI GAGTC 4 cut(s) 249, 646, 684, 932
PpsI GAGTC 4 cut(s) 249, 646, 684, 932
PpuMI RGGWCCY 1 cut(s) 752
PsiI TTATAA 1 cut(s) 288
Psp5II RGGWCCY 1 cut(s) 752
Psp6I CCWGG 2 cut(s) 27, 425
PspGI CCWGG 2 cut(s) 27, 425
PspN4I GGNNCC 2 cut(s) 33, 753
PspPI GGNCC 2 cut(s) 31, 752
PspPPI RGGWCCY 1 cut(s) 752
RsaI GTAC 3 cut(s) 424, 485, 715
RsaNI GTAC 3 cut(s) 423, 484, 714
RseI CAYNNNNRTG 1 cut(s) 741
SaqAI TTAA 4 cut(s) 360, 545, 731, 834
SatI GCNGC 3 cut(s) 135, 138, 350
Sau3AI GATC 5 cut(s) 432, 796, 820, 855, 964
Sau96I GGNCC 2 cut(s) 31, 752
ScaI AGTACT 1 cut(s) 715
SchI GAGTC 4 cut(s) 250, 647, 685, 932
ScrFI CCNGG 2 cut(s) 29, 427
SfaNI GCATC 1 cut(s) 966
Sfr274I CTCGAG 1 cut(s) 919
SinI GGWCC 2 cut(s) 31, 752
SlaI CTCGAG 1 cut(s) 919
SmiMI CAYNNNNRTG 1 cut(s) 741
SmlI CTYRAG 1 cut(s) 919
SmoI CTYRAG 1 cut(s) 919
Sse9I AATT 6 cut(s) 159, 217, 283, 361, 532, 732
SsiI CCGC 2 cut(s) 138, 896
SspMI CTAG 4 cut(s) 227, 642, 687, 711
StyD4I CCNGG 2 cut(s) 27, 425
TaaI ACNGT 2 cut(s) 465, 891
TaiI ACGT 1 cut(s) 409
TaqI TCGA 3 cut(s) 698, 823, 920
TasI AATT 6 cut(s) 159, 217, 283, 361, 532, 732
TatI WGTACW 1 cut(s) 713
TauI GCSGC 1 cut(s) 140
TfiI GAWTC 2 cut(s) 259, 334
Tru1I TTAA 4 cut(s) 360, 545, 731, 834
Tru9I TTAA 4 cut(s) 360, 545, 731, 834
TseI GCWGC 2 cut(s) 134, 349
TspDTI ATGAA 9 cut(s) 135, 140, 222, 606, 614, 695, 725, 782, 820
TspGWI ACGGA 2 cut(s) 271, 950
Van91I CCANNNNNTGG 1 cut(s) 590
VpaK11BI GGWCC 2 cut(s) 31, 752
XapI RAATTY 3 cut(s) 159, 217, 532
XceI RCATGY 2 cut(s) 484, 812
XcmI CCANNNNNNNNNTGG 1 cut(s) 433
XhoI CTCGAG 1 cut(s) 919
XspI CTAG 4 cut(s) 227, 642, 687, 711
ZrmI AGTACT 1 cut(s) 715
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.