Rw4G033260
RLK Family

Cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000146
Physical Location & Seq
Forward (+)
60339482 .. 60340381
900 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G033260.1

Sequence Viewer

Length: 741 bp
ATGGTTCTCAATGCAGTACCCACTGATTCATCCCACACATCAGCAACCAACTCCGAATTTTCTTCTCAACATAAAGGCCAATCGTCATCCAATGATCATGGCCTTATTGCTACACAAAAATTAGATAGCACCAATTTTATTTCTTGGTCTAAACAAGCCAAGTTGAACATCATCGGTCGAGATAAACTTGGCTATCTCACCAGCAAGAAGAAAGCACCATCTGAAGAAGAATATGAGGAGGGTTATGAAATTTGGATTGAAGAGGACAGTCTTGTTCAAGCATGGCTCCTCAACTGCATGCACAAGGATATCAAACGAAATTATGAAAGCTTGGAGACAGCTAAAGAAATTTGGGTGCTAAACTATGAGCTGCAAATTGCTTCTGTTTCCACTAAACATAATGGAGCTCCTCTTTATGGCTATTATAGCAATATGAAGAAAATATGGCAGGAGCTTGACATATTGGATCCTCTCTCCTTCACTAAGCACTGCATCTATTTCTTTTTGGCTGGGCTAAACCCTCACCTTGATGGAGTTCGGAGTAGAATCCTAAACACCAAGCCATTGCCTTCCATAGAAAAGGCTTATTCTCAAGTCAGTGTTGAGCATACTAGACTACAAAAGATGATGCCTGATACAAAAAAAACACCTTCTCCAGGACCCATGAAATGCACTCATTGCAAATTAACTAGGCATATGGTAGAGACTTGTGTCCAATTACATGGCTTTCCAAAATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

246

Amino Acids

28.13

Weight (kDa)

8.17

Isoelectric Point (pI)

51.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000146)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19561 FvH4_1g25371 FvH4_1g25961 FvH4_2g00352 FvH4_2g00861 FvH4_2g04952 FvH4_2g05413 FvH4_2g07531 FvH4_2g08961 FvH4_2g25141 FvH4_2g27292 FvH4_3g18164 FvH4_3g19921 FvH4_3g43870 FvH4_4g14441 FvH4_4g15581 FvH4_4g15582 FvH4_4g16890 FvH4_4g19782 FvH4_4g33444 FvH4_4g33921 FvH4_5g22281 FvH4_5g22282 FvH4_5g32571 FvH4_5g37451 FvH4_5g39631 FvH4_6g04721 FvH4_6g11661 FvH4_6g20571 FvH4_6g32791 FvH4_7g23184
prunus_persica Prupe.1G090900_v2.0.a1 Prupe.1G241200_v2.0.a1 Prupe.1G534100_v2.0.a1 Prupe.3G076700_v2.0.a1 Prupe.4G150800_v2.0.a1 Prupe.4G181800_v2.0.a1 Prupe.4G240900_v2.0.a1 Prupe.4G247600_v2.0.a1 Prupe.6G115900_v2.0.a1 Prupe.6G327500_v2.0.a1 Prupe.8G071700_v2.0.a1
pyrus_communis pycom06g11480 pycom10g08330 pycom15g04320 pycom15g04350 pycom15g12620 pycom2874g00060
rosa_chinensis RchiOBHm_Chr1g0317241 RchiOBHm_Chr1g0341801 RchiOBHm_Chr1g0341931 RchiOBHm_Chr1g0359621 RchiOBHm_Chr2g0113741 RchiOBHm_Chr2g0117261 RchiOBHm_Chr2g0138831 RchiOBHm_Chr2g0141421 RchiOBHm_Chr2g0145281 RchiOBHm_Chr2g0152991 RchiOBHm_Chr3g0448001 RchiOBHm_Chr3g0450981 RchiOBHm_Chr3g0455161 RchiOBHm_Chr3g0457401 RchiOBHm_Chr3g0460001 RchiOBHm_Chr4g0387431 RchiOBHm_Chr4g0388741 RchiOBHm_Chr4g0444201 RchiOBHm_Chr4g0444261 RchiOBHm_Chr5g0010221 RchiOBHm_Chr6g0271721 RchiOBHm_Chr6g0274641 RchiOBHm_Chr6g0294691 RchiOBHm_Chr7g0237031 RchiOBHm_Chr7g0238461 RchiOBHm_Chr7g0239981
rosa_laevigata RLG00000009017
rosa_multiflora Rmu_co7986768.1_g000001 Rmu_co8302239.1_g000001 Rmu_co8485799.1_g000001 Rmu_sc0000061.1_g000013 Rmu_sc0000303.1_g000034 Rmu_sc0000303.1_g000035 Rmu_sc0000315.1_g000017 Rmu_sc0000349.1_g000003 Rmu_sc0000498.1_g000068 Rmu_sc0000508.1_g000014 Rmu_sc0000602.1_g000001 Rmu_sc0000633.1_g000002 Rmu_sc0000638.1_g000030 Rmu_sc0000756.1_g000016 Rmu_sc0000756.1_g000017 Rmu_sc0000785.1_g000037 Rmu_sc0000795.1_g000017 Rmu_sc0000796.1_g000030 Rmu_sc0000863.1_g000023 Rmu_sc0000920.1_g000021 Rmu_sc0000960.1_g000015 Rmu_sc0000982.1_g000102 Rmu_sc0001036.1_g000008 Rmu_sc0001036.1_g000009 Rmu_sc0001643.1_g000029 Rmu_sc0001687.1_g000026 Rmu_sc0001708.1_g000037 Rmu_sc0001768.1_g000015 Rmu_sc0001790.1_g000022 Rmu_sc0002180.1_g000007 Rmu_sc0002211.1_g000006 Rmu_sc0002270.1_g000010 Rmu_sc0002404.1_g000031 Rmu_sc0002858.1_g000016 Rmu_sc0003063.1_g000028 Rmu_sc0003730.1_g000017 Rmu_sc0004087.1_g000020 Rmu_sc0004618.1_g000048 Rmu_sc0004724.1_g000005 Rmu_sc0005167.1_g000036 Rmu_sc0005781.1_g000003 Rmu_sc0005878.1_g000012 Rmu_sc0006011.1_g000003 Rmu_sc0006343.1_g000011 Rmu_sc0006573.1_g000007 Rmu_sc0006599.1_g000007 Rmu_sc0006803.1_g000014 Rmu_sc0006836.1_g000002 Rmu_sc0006849.1_g000002 Rmu_sc0007109.1_g000009 Rmu_sc0009268.1_g000016 Rmu_sc0009618.1_g000017 Rmu_sc0009674.1_g000009 Rmu_sc0010244.1_g000009 Rmu_sc0011151.1_g000004 Rmu_sc0011356.1_g000001 Rmu_sc0011674.1_g000002 Rmu_sc0011778.1_g000016 Rmu_sc0012342.1_g000001 Rmu_sc0012467.1_g000002 Rmu_sc0012574.1_g000003 Rmu_sc0012913.1_g000005 Rmu_sc0013612.1_g000020 Rmu_sc0014824.1_g000001 Rmu_sc0017869.1_g000002 Rmu_sc0018085.1_g000003 Rmu_sc0024789.1_g000001 Rmu_sc0025106.1_g000013 Rmu_sc0034502.1_g000001 Rmu_sc0034547.1_g000001 Rmu_sc0035094.1_g000001 Rmu_sc0035095.1_g000001 Rmu_ssc0000100.1_g000027 Rmu_ssc0000244.1_g000005 Rmu_ssc0000371.1_g000029 Rmu_ssc0000381.1_g000004
rosa_roxburghii Rroxscaffold_1G00015480 Rroxscaffold_1G00023580 Rroxscaffold_1G00039340 Rroxscaffold_1G00047080 Rroxscaffold_2G00095820 Rroxscaffold_2G00125200 Rroxscaffold_2G00128330 Rroxscaffold_3G00270860
rosa_rugosa Rorug06G0120700
rosa_samantha Rh3BG173100 Rh6AG089600 Rh7AG488100 Rh7DG389600
rosa_wichuraiana Rw0G001150 Rw0G001990 Rw0G002010 Rw0G019540 Rw1G013730 Rw1G024110 Rw1G029190 Rw2G018850 Rw2G021090 Rw2G022260 Rw2G023180 Rw2G027280 Rw3G010190 Rw3G020660 Rw3G029380 Rw4G002390 Rw4G002690 Rw4G033260 Rw5G011960 Rw5G023110 Rw5G025250 Rw5G028950 Rw5G033740 Rw5G033950 Rw5G037900 Rw6G009640 Rw6G011620 Rw6G027720 Rw7G010680 Rw7G042070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 461, 474
AcsI RAATTY 3 cut(s) 56, 249, 348
AcuI CTGAAG 1 cut(s) 243
AfaI GTAC 1 cut(s) 18
AfiI CCNNNNNNNGG 2 cut(s) 416, 656
AgsI TTSAA 3 cut(s) 166, 260, 278
AjnI CCWGG 1 cut(s) 655
AluBI AGCT 5 cut(s) 330, 341, 370, 407, 454
AluI AGCT 5 cut(s) 330, 341, 370, 407, 454
Alw21I GWGCWC 1 cut(s) 409
Alw26I GTCTC 2 cut(s) 329, 698
AlwI GGATC 2 cut(s) 461, 474
AoxI GGCC 2 cut(s) 76, 100
ApeKI GCWGC 1 cut(s) 370
ApoI RAATTY 3 cut(s) 56, 249, 348
AspS9I GGNCC 1 cut(s) 659
AsuHPI GGTGA 2 cut(s) 190, 515
AvaII GGWCC 1 cut(s) 659
BamHI GGATCC 1 cut(s) 466
BanII GRGCYC 1 cut(s) 409
Bbv12I GWGCWC 1 cut(s) 409
BbvI GCAGC 1 cut(s) 357
BccI CCATC 2 cut(s) 226, 524
BciT130I CCWGG 1 cut(s) 657
BclI TGATCA 1 cut(s) 94
BcoDI GTCTC 2 cut(s) 329, 698
BfaI CTAG 2 cut(s) 612, 690
BisI GCNGC 1 cut(s) 371
BlsI GCNGC 1 cut(s) 372
Bme1390I CCNGG 1 cut(s) 657
Bme18I GGWCC 1 cut(s) 659
BmgT120I GGNCC 1 cut(s) 659
BmiI GGNNCC 3 cut(s) 287, 468, 661
BmrFI CCNGG 1 cut(s) 657
BmsI GCATC 2 cut(s) 501, 618
BoxI GACNNNNGTC 1 cut(s) 710
BpmI CTGGAG 1 cut(s) 639
BpuEI CTTGAG 1 cut(s) 576
BsaXI ACNNNNNCTCC 2 cut(s) 637, 667
Bsc4I CCNNNNNNNGG 2 cut(s) 416, 656
Bse3DI GCAATG 2 cut(s) 563, 676
BseBI CCWGG 1 cut(s) 657
BseGI GGATG 2 cut(s) 29, 86
BseLI CCNNNNNNNGG 2 cut(s) 416, 656
BseMI GCAATG 2 cut(s) 563, 676
BseRI GAGGAG 3 cut(s) 251, 278, 399
BseXI GCAGC 1 cut(s) 357
BseYI CCCAGC 1 cut(s) 509
Bsh1285I CGRYCG 1 cut(s) 178
BshFI GGCC 2 cut(s) 78, 102
BsiEI CGRYCG 1 cut(s) 178
BsiHKAI GWGCWC 1 cut(s) 409
BslI CCNNNNNNNGG 2 cut(s) 416, 656
BsmAI GTCTC 2 cut(s) 329, 698
BsnI GGCC 2 cut(s) 78, 102
Bsp1286I GDGCHC 1 cut(s) 409
Bsp143I GATC 2 cut(s) 94, 466
BspANI GGCC 2 cut(s) 78, 102
BspLI GGNNCC 3 cut(s) 287, 468, 661
BspPI GGATC 2 cut(s) 461, 474
BsrDI GCAATG 2 cut(s) 563, 676
BssMI GATC 2 cut(s) 94, 466
Bst2UI CCWGG 1 cut(s) 657
Bst4CI ACNGT 1 cut(s) 269
Bst6I CTCTTC 1 cut(s) 255
BstAPI GCANNNNNTGC 1 cut(s) 678
BstC8I GCNNGC 1 cut(s) 299
BstDEI CTNAG 1 cut(s) 483
BstENI CCTNNNNNAGG 1 cut(s) 654
BstF5I GGATG 2 cut(s) 29, 86
BstKTI GATC 2 cut(s) 97, 469
BstMAI GTCTC 2 cut(s) 329, 698
BstMBI GATC 2 cut(s) 94, 466
BstMCI CGRYCG 1 cut(s) 178
BstMWI GCNNNNNNNGC 2 cut(s) 426, 678
BstNI CCWGG 1 cut(s) 657
BstNSI RCATGY 1 cut(s) 301
BstPAI GACNNNNGTC 1 cut(s) 710
BstSCI CCNGG 1 cut(s) 655
BstV1I GCAGC 1 cut(s) 357
BstX2I RGATCY 1 cut(s) 466
BstXI CCANNNNNNTGG 1 cut(s) 722
BstYI RGATCY 1 cut(s) 466
BsuRI GGCC 2 cut(s) 78, 102
BtsCI GGATG 2 cut(s) 29, 86
BtsI GCAGTG 1 cut(s) 487
BtsIMutI CAGTG 3 cut(s) 21, 487, 604
Cac8I GCNNGC 1 cut(s) 299
Cfr13I GGNCC 1 cut(s) 659
Csp6I GTAC 1 cut(s) 17
CviAII CATG 5 cut(s) 98, 282, 298, 664, 722
CviQI GTAC 1 cut(s) 17
DdeI CTNAG 1 cut(s) 483
DpnI GATC 2 cut(s) 96, 468
DpnII GATC 2 cut(s) 94, 466
Eam1104I CTCTTC 1 cut(s) 255
EarI CTCTTC 1 cut(s) 255
Ecl136II GAGCTC 1 cut(s) 407
Eco24I GRGCYC 1 cut(s) 409
Eco32I GATATC 1 cut(s) 310
Eco47I GGWCC 1 cut(s) 659
Eco53kI GAGCTC 1 cut(s) 407
Eco57I CTGAAG 1 cut(s) 243
EcoICRI GAGCTC 1 cut(s) 407
EcoNI CCTNNNNNAGG 1 cut(s) 654
EcoO109I RGGNCCY 1 cut(s) 659
EcoRII CCWGG 1 cut(s) 655
EcoRV GATATC 1 cut(s) 310
EcoT38I GRGCYC 1 cut(s) 409
FaeI CATG 5 cut(s) 101, 285, 301, 667, 725
FatI CATG 5 cut(s) 97, 281, 297, 663, 721
FauNDI CATATG 1 cut(s) 696
FbaI TGATCA 1 cut(s) 94
Fnu4HI GCNGC 1 cut(s) 371
FokI GGATG 2 cut(s) 16, 73
FriOI GRGCYC 1 cut(s) 409
Fsp4HI GCNGC 1 cut(s) 371
FspBI CTAG 2 cut(s) 612, 690
GluI GCNGC 1 cut(s) 371
GsaI CCCAGC 1 cut(s) 513
GsuI CTGGAG 1 cut(s) 639
HaeIII GGCC 2 cut(s) 78, 102
Hin1II CATG 5 cut(s) 101, 285, 301, 667, 725
HindIII AAGCTT 1 cut(s) 328
HinfI GANTC 2 cut(s) 26, 546
HphI GGTGA 2 cut(s) 190, 515
Hpy188I TCNGA 3 cut(s) 55, 223, 540
Hpy188III TCNNGA 1 cut(s) 179
HpyAV CCTTC 3 cut(s) 487, 579, 660
HpyCH4III ACNGT 1 cut(s) 269
HpyCH4V TGCA 7 cut(s) 14, 297, 301, 373, 492, 672, 681
HpyF10VI GCNNNNNNNGC 2 cut(s) 426, 678
HpyF3I CTNAG 1 cut(s) 483
Hsp92II CATG 5 cut(s) 101, 285, 301, 667, 725
Ksp22I TGATCA 1 cut(s) 94
Kzo9I GATC 2 cut(s) 94, 466
LmnI GCTCC 4 cut(s) 291, 404, 412, 451
LpnPI CCDG 6 cut(s) 214, 434, 495, 642, 645, 669
Lsp1109I GCAGC 1 cut(s) 357
LweI GCATC 2 cut(s) 501, 618
MaeI CTAG 2 cut(s) 612, 690
MalI GATC 2 cut(s) 96, 468
MboI GATC 2 cut(s) 94, 466
MboII GAAGA 6 cut(s) 54, 220, 236, 239, 272, 448
MflI RGATCY 1 cut(s) 466
MhlI GDGCHC 1 cut(s) 409
MluCI AATT 9 cut(s) 56, 119, 133, 249, 319, 348, 375, 683, 716
MnlI CCTC 7 cut(s) 229, 232, 256, 299, 420, 480, 531
MseI TTAA 1 cut(s) 686
MslI CAYNNNNRTG 1 cut(s) 528
MspR9I CCNGG 1 cut(s) 657
MvaI CCWGG 1 cut(s) 657
MwoI GCNNNNNNNGC 2 cut(s) 426, 678
NdeI CATATG 1 cut(s) 696
NdeII GATC 2 cut(s) 94, 466
NlaIII CATG 5 cut(s) 101, 285, 301, 667, 725
NlaIV GGNNCC 3 cut(s) 287, 468, 661
NspI RCATGY 1 cut(s) 301
PaeI GCATGC 1 cut(s) 301
PfeI GAWTC 2 cut(s) 26, 546
PfoI TCCNGGA 1 cut(s) 655
PkrI GCNGC 1 cut(s) 372
PpuMI RGGWCCY 1 cut(s) 659
PshAI GACNNNNGTC 1 cut(s) 710
Psp124BI GAGCTC 1 cut(s) 409
Psp5II RGGWCCY 1 cut(s) 659
Psp6I CCWGG 1 cut(s) 655
PspFI CCCAGC 1 cut(s) 509
PspGI CCWGG 1 cut(s) 655
PspN4I GGNNCC 3 cut(s) 287, 468, 661
PspPI GGNCC 1 cut(s) 659
PspPPI RGGWCCY 1 cut(s) 659
PsuI RGATCY 1 cut(s) 466
RsaI GTAC 1 cut(s) 18
RsaNI GTAC 1 cut(s) 17
RseI CAYNNNNRTG 1 cut(s) 528
SacI GAGCTC 1 cut(s) 409
SaqAI TTAA 1 cut(s) 686
SatI GCNGC 1 cut(s) 371
Sau3AI GATC 2 cut(s) 94, 466
Sau96I GGNCC 1 cut(s) 659
ScrFI CCNGG 1 cut(s) 657
SduI GDGCHC 1 cut(s) 409
SetI ASST 7 cut(s) 332, 343, 372, 409, 456, 528, 652
SfaNI GCATC 2 cut(s) 501, 618
SinI GGWCC 1 cut(s) 659
SmiMI CAYNNNNRTG 1 cut(s) 528
SmlI CTYRAG 1 cut(s) 591
SmoI CTYRAG 1 cut(s) 591
SphI GCATGC 1 cut(s) 301
Sse9I AATT 9 cut(s) 56, 119, 133, 249, 319, 348, 375, 683, 716
SspMI CTAG 2 cut(s) 612, 690
SstI GAGCTC 1 cut(s) 409
StyD4I CCNGG 1 cut(s) 655
TaaI ACNGT 1 cut(s) 269
TaqI TCGA 1 cut(s) 178
TaqII GACCGA 1 cut(s) 164
TasI AATT 9 cut(s) 56, 119, 133, 249, 319, 348, 375, 683, 716
TfiI GAWTC 2 cut(s) 26, 546
Tru1I TTAA 1 cut(s) 686
Tru9I TTAA 1 cut(s) 686
TscAI CASTG 3 cut(s) 28, 494, 604
TseI GCWGC 1 cut(s) 370
TspDTI ATGAA 5 cut(s) 18, 261, 339, 449, 680
TspRI CASTG 3 cut(s) 28, 494, 604
VpaK11BI GGWCC 1 cut(s) 659
XagI CCTNNNNNAGG 1 cut(s) 654
XapI RAATTY 3 cut(s) 56, 249, 348
XceI RCATGY 1 cut(s) 301
XspI CTAG 2 cut(s) 612, 690
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.