FvH4_2g25141
RLK Family

Cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000146
Physical Location & Seq
Reverse (-)
20408686 .. 20411699
3014 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g25141.t1

Sequence Viewer

Length: 996 bp
ATGGATTCTGGAGTTGAAAGTTCTCAGGGGAAAGATGTTCAGACAGTTGAAGTATCTGTCAAGAATTCTGAAAGTGGTTCATTTGGGGGTGCCAAACTCAATGGAACCAATTTTCGGAAATGGAAGAAAATCATGACGGCTCATCTTCGAGGGATGCACAAGATGGGGCATGTAACTGGGACAATTAAGGCCCCTAGTGAAAGTGATGTTGATGCTTACACTAAGTGGGAAGATGATGATGGTCTTGTGATGTCAGTCTTGTACAAGGCCATGAATGAGGACATAGTCGACCTGGTGGAACAATGTGATACAGCTCAGGCGATCTGGAAGACACTGGAGGATTTATATACTAATGAGTCTGATTTCATACAGGTTCATGAGTTGATGTGCACTGCTTCCTCAATGCAACAGCATGGGCAGCCAGTGGCACAGTATTTCACCAAGCTAAGGAACATTTGGGCTGAAATTGATACGAAACGTCCTTGCAAGATCAAGAACCAAGAGGATCTTGTTTGGTACTTGAAGGAAAAGGAGCTTGAGCGAGTTCATCACTTTCTGAGGGGTCTTGATGCAAAACACAACAGTGCCAAAGGAGAGCTACTCAGAATGACCGAACCTCCTAACCTGATTACAGCTTTCACATATATCCGGGAGCATTCATATTTTGAAGAGCTGTATCATGGAGAGGGGGAGACAGGTCATTCAGTTGAGGTTACTGATGCATCAGCTTCACATACACCAGCCACTCCAGAGGTTCAGAGACCAGAAGATGCTAGACAGGCACCACCAGATGATTCTGGTGCAATTGCTCCAGAGATTCAGGAACCAGAAGCTGAAAGTGTTCCTGCCCCTCCTGCCTCGCCTGCTGTTACTAATGCCCCTGACTCATGTCTCCCTGGTACAGAAGATCTTCCATCTGAGGTTAGTGATTCTATTGGGGTTAATGCTAGTGATGCTAATAGTAGACAATATGTGTTACCCAATAGTGAAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Retrotran_gag_2

Protein Analysis

332

Amino Acids

36.6

Weight (kDa)

4.79

Isoelectric Point (pI)

46.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotran_gag_2 PF14223 45 - 157 2.5e-12 gag-polypeptide of LTR copia-type
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000146)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19561 FvH4_1g25371 FvH4_1g25961 FvH4_2g00352 FvH4_2g00861 FvH4_2g04952 FvH4_2g05413 FvH4_2g07531 FvH4_2g08961 FvH4_2g25141 FvH4_2g27292 FvH4_3g18164 FvH4_3g19921 FvH4_3g43870 FvH4_4g14441 FvH4_4g15581 FvH4_4g15582 FvH4_4g16890 FvH4_4g19782 FvH4_4g33444 FvH4_4g33921 FvH4_5g22281 FvH4_5g22282 FvH4_5g32571 FvH4_5g37451 FvH4_5g39631 FvH4_6g04721 FvH4_6g11661 FvH4_6g20571 FvH4_6g32791 FvH4_7g23184
prunus_persica Prupe.1G090900_v2.0.a1 Prupe.1G241200_v2.0.a1 Prupe.1G534100_v2.0.a1 Prupe.3G076700_v2.0.a1 Prupe.4G150800_v2.0.a1 Prupe.4G181800_v2.0.a1 Prupe.4G240900_v2.0.a1 Prupe.4G247600_v2.0.a1 Prupe.6G115900_v2.0.a1 Prupe.6G327500_v2.0.a1 Prupe.8G071700_v2.0.a1
pyrus_communis pycom06g11480 pycom10g08330 pycom15g04320 pycom15g04350 pycom15g12620 pycom2874g00060
rosa_chinensis RchiOBHm_Chr1g0317241 RchiOBHm_Chr1g0341801 RchiOBHm_Chr1g0341931 RchiOBHm_Chr1g0359621 RchiOBHm_Chr2g0113741 RchiOBHm_Chr2g0117261 RchiOBHm_Chr2g0138831 RchiOBHm_Chr2g0141421 RchiOBHm_Chr2g0145281 RchiOBHm_Chr2g0152991 RchiOBHm_Chr3g0448001 RchiOBHm_Chr3g0450981 RchiOBHm_Chr3g0455161 RchiOBHm_Chr3g0457401 RchiOBHm_Chr3g0460001 RchiOBHm_Chr4g0387431 RchiOBHm_Chr4g0388741 RchiOBHm_Chr4g0444201 RchiOBHm_Chr4g0444261 RchiOBHm_Chr5g0010221 RchiOBHm_Chr6g0271721 RchiOBHm_Chr6g0274641 RchiOBHm_Chr6g0294691 RchiOBHm_Chr7g0237031 RchiOBHm_Chr7g0238461 RchiOBHm_Chr7g0239981
rosa_laevigata RLG00000009017
rosa_multiflora Rmu_co7986768.1_g000001 Rmu_co8302239.1_g000001 Rmu_co8485799.1_g000001 Rmu_sc0000061.1_g000013 Rmu_sc0000303.1_g000034 Rmu_sc0000303.1_g000035 Rmu_sc0000315.1_g000017 Rmu_sc0000349.1_g000003 Rmu_sc0000498.1_g000068 Rmu_sc0000508.1_g000014 Rmu_sc0000602.1_g000001 Rmu_sc0000633.1_g000002 Rmu_sc0000638.1_g000030 Rmu_sc0000756.1_g000016 Rmu_sc0000756.1_g000017 Rmu_sc0000785.1_g000037 Rmu_sc0000795.1_g000017 Rmu_sc0000796.1_g000030 Rmu_sc0000863.1_g000023 Rmu_sc0000920.1_g000021 Rmu_sc0000960.1_g000015 Rmu_sc0000982.1_g000102 Rmu_sc0001036.1_g000008 Rmu_sc0001036.1_g000009 Rmu_sc0001643.1_g000029 Rmu_sc0001687.1_g000026 Rmu_sc0001708.1_g000037 Rmu_sc0001768.1_g000015 Rmu_sc0001790.1_g000022 Rmu_sc0002180.1_g000007 Rmu_sc0002211.1_g000006 Rmu_sc0002270.1_g000010 Rmu_sc0002404.1_g000031 Rmu_sc0002858.1_g000016 Rmu_sc0003063.1_g000028 Rmu_sc0003730.1_g000017 Rmu_sc0004087.1_g000020 Rmu_sc0004618.1_g000048 Rmu_sc0004724.1_g000005 Rmu_sc0005167.1_g000036 Rmu_sc0005781.1_g000003 Rmu_sc0005878.1_g000012 Rmu_sc0006011.1_g000003 Rmu_sc0006343.1_g000011 Rmu_sc0006573.1_g000007 Rmu_sc0006599.1_g000007 Rmu_sc0006803.1_g000014 Rmu_sc0006836.1_g000002 Rmu_sc0006849.1_g000002 Rmu_sc0007109.1_g000009 Rmu_sc0009268.1_g000016 Rmu_sc0009618.1_g000017 Rmu_sc0009674.1_g000009 Rmu_sc0010244.1_g000009 Rmu_sc0011151.1_g000004 Rmu_sc0011356.1_g000001 Rmu_sc0011674.1_g000002 Rmu_sc0011778.1_g000016 Rmu_sc0012342.1_g000001 Rmu_sc0012467.1_g000002 Rmu_sc0012574.1_g000003 Rmu_sc0012913.1_g000005 Rmu_sc0013612.1_g000020 Rmu_sc0014824.1_g000001 Rmu_sc0017869.1_g000002 Rmu_sc0018085.1_g000003 Rmu_sc0024789.1_g000001 Rmu_sc0025106.1_g000013 Rmu_sc0034502.1_g000001 Rmu_sc0034547.1_g000001 Rmu_sc0035094.1_g000001 Rmu_sc0035095.1_g000001 Rmu_ssc0000100.1_g000027 Rmu_ssc0000244.1_g000005 Rmu_ssc0000371.1_g000029 Rmu_ssc0000381.1_g000004
rosa_roxburghii Rroxscaffold_1G00015480 Rroxscaffold_1G00023580 Rroxscaffold_1G00039340 Rroxscaffold_1G00047080 Rroxscaffold_2G00095820 Rroxscaffold_2G00125200 Rroxscaffold_2G00128330 Rroxscaffold_3G00270860
rosa_rugosa Rorug06G0120700
rosa_samantha Rh3BG173100 Rh6AG089600 Rh7AG488100 Rh7DG389600
rosa_wichuraiana Rw0G001150 Rw0G001990 Rw0G002010 Rw0G019540 Rw1G013730 Rw1G024110 Rw1G029190 Rw2G018850 Rw2G021090 Rw2G022260 Rw2G023180 Rw2G027280 Rw3G010190 Rw3G020660 Rw3G029380 Rw4G002390 Rw4G002690 Rw4G033260 Rw5G011960 Rw5G023110 Rw5G025250 Rw5G028950 Rw5G033740 Rw5G033950 Rw5G037900 Rw6G009640 Rw6G011620 Rw6G027720 Rw7G010680 Rw7G042070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 89, 781
AccI GTMKAC 2 cut(s) 288, 964
AclWI GGATC 1 cut(s) 513
AcsI RAATTY 1 cut(s) 64
AdeI CACNNNGTG 1 cut(s) 225
AfaI GTAC 3 cut(s) 263, 518, 901
AfiI CCNNNNNNNGG 2 cut(s) 114, 447
AgsI TTSAA 4 cut(s) 17, 50, 523, 668
AjnI CCWGG 2 cut(s) 291, 895
AleI CACNNNNGTG 1 cut(s) 582
AluBI AGCT 8 cut(s) 314, 445, 535, 598, 635, 673, 728, 833
AluI AGCT 8 cut(s) 314, 445, 535, 598, 635, 673, 728, 833
Alw21I GWGCWC 1 cut(s) 392
Alw26I GTCTC 3 cut(s) 686, 754, 896
Alw44I GTGCAC 1 cut(s) 388
AlwI GGATC 1 cut(s) 513
AlwNI CAGNNNCTG 1 cut(s) 833
AoxI GGCC 2 cut(s) 189, 267
ApaLI GTGCAC 1 cut(s) 388
ApeKI GCWGC 1 cut(s) 418
ApoI RAATTY 1 cut(s) 64
Asp700I GAANNNNTTC 2 cut(s) 840, 909
AspS9I GGNCC 1 cut(s) 190
AsuC2I CCSGG 1 cut(s) 650
AsuHPI GGTGA 1 cut(s) 430
BaeGI GKGCMC 1 cut(s) 392
BanI GGYRCC 2 cut(s) 89, 781
BbsI GAAGAC 1 cut(s) 335
Bbv12I GWGCWC 1 cut(s) 392
BbvI GCAGC 1 cut(s) 430
BccI CCATC 3 cut(s) 157, 233, 922
BceAI ACGGC 1 cut(s) 153
BciT130I CCWGG 2 cut(s) 293, 897
BcnI CCSGG 1 cut(s) 650
BcoDI GTCTC 3 cut(s) 686, 754, 896
BfaI CTAG 3 cut(s) 195, 774, 948
BglII AGATCT 1 cut(s) 907
BisI GCNGC 1 cut(s) 419
BlsI GCNGC 1 cut(s) 420
Bme1390I CCNGG 3 cut(s) 293, 650, 897
BmgT120I GGNCC 1 cut(s) 190
BmiI GGNNCC 5 cut(s) 91, 106, 192, 783, 825
BmrFI CCNGG 3 cut(s) 293, 650, 897
BmrI ACTGGG 1 cut(s) 186
BmsI GCATC 7 cut(s) 144, 202, 559, 709, 731, 760, 943
BmuI ACTGGG 1 cut(s) 186
BoxI GACNNNNGTC 1 cut(s) 888
BpiI GAAGAC 1 cut(s) 335
BplI GAGNNNNNCTC 2 cut(s) 585, 617
BpmI CTGGAG 4 cut(s) 30, 356, 732, 795
Bpu10I CCTNAGC 2 cut(s) 315, 446
BpuEI CTTGAG 1 cut(s) 557
BpuMI CCSGG 1 cut(s) 650
BsaI GGTCTC 1 cut(s) 754
BsaJI CCNNGG 1 cut(s) 895
BsaXI ACNNNNNCTCC 2 cut(s) 601, 631
Bsc4I CCNNNNNNNGG 2 cut(s) 114, 447
Bse1I ACTGG 3 cut(s) 181, 339, 422
BseBI CCWGG 2 cut(s) 293, 897
BseDI CCNNGG 1 cut(s) 895
BseGI GGATG 1 cut(s) 159
BseLI CCNNNNNNNGG 2 cut(s) 114, 447
BseMII CTCAG 5 cut(s) 38, 329, 548, 616, 909
BseNI ACTGG 3 cut(s) 181, 339, 422
BseSI GKGCMC 1 cut(s) 392
BseXI GCAGC 1 cut(s) 430
BshFI GGCC 2 cut(s) 191, 269
BshNI GGYRCC 2 cut(s) 89, 781
BsiHKAI GWGCWC 1 cut(s) 392
BsiSI CCGG 1 cut(s) 649
BslFI GGGAC 1 cut(s) 193
BslI CCNNNNNNNGG 2 cut(s) 114, 447
BsmAI GTCTC 3 cut(s) 686, 754, 896
BsmFI GGGAC 1 cut(s) 193
BsmI GAATGC 1 cut(s) 655
BsnI GGCC 2 cut(s) 191, 269
Bso31I GGTCTC 1 cut(s) 754
Bsp1286I GDGCHC 1 cut(s) 392
Bsp1407I TGTACA 1 cut(s) 261
Bsp143I GATC 4 cut(s) 321, 489, 505, 907
BspANI GGCC 2 cut(s) 191, 269
BspCNI CTCAG 5 cut(s) 37, 328, 549, 615, 910
BspHI TCATGA 2 cut(s) 132, 376
BspLI GGNNCC 5 cut(s) 91, 106, 192, 783, 825
BspPI GGATC 1 cut(s) 513
BspQI GCTCTTC 1 cut(s) 663
BspT107I GGYRCC 2 cut(s) 89, 781
BspTNI GGTCTC 1 cut(s) 754
BsrGI TGTACA 1 cut(s) 261
BsrI ACTGG 3 cut(s) 181, 339, 422
BssECI CCNNGG 1 cut(s) 895
BssMI GATC 4 cut(s) 321, 489, 505, 907
Bst2UI CCWGG 2 cut(s) 293, 897
Bst4CI ACNGT 3 cut(s) 46, 432, 584
Bst6I CTCTTC 1 cut(s) 663
BstAUI TGTACA 1 cut(s) 261
BstC8I GCNNGC 1 cut(s) 864
BstDEI CTNAG 7 cut(s) 24, 222, 315, 446, 557, 602, 918
BstF5I GGATG 1 cut(s) 159
BstKTI GATC 4 cut(s) 324, 492, 508, 910
BstMAI GTCTC 3 cut(s) 686, 754, 896
BstMBI GATC 4 cut(s) 321, 489, 505, 907
BstMWI GCNNNNNNNGC 5 cut(s) 418, 779, 854, 863, 953
BstNI CCWGG 2 cut(s) 293, 897
BstNSI RCATGY 1 cut(s) 173
BstPAI GACNNNNGTC 1 cut(s) 888
BstSCI CCNGG 3 cut(s) 291, 648, 895
BstSLI GKGCMC 1 cut(s) 392
BstV1I GCAGC 1 cut(s) 430
BstV2I GAAGAC 1 cut(s) 335
BstX2I RGATCY 2 cut(s) 505, 907
BstYI RGATCY 2 cut(s) 505, 907
BsuRI GGCC 2 cut(s) 191, 269
BtsCI GGATG 1 cut(s) 159
BtsI GCAGTG 1 cut(s) 390
BtsIMutI CAGTG 4 cut(s) 332, 390, 429, 589
Cac8I GCNNGC 1 cut(s) 864
CaiI CAGNNNCTG 1 cut(s) 833
CciI TCATGA 2 cut(s) 132, 376
Cfr13I GGNCC 1 cut(s) 190
CsiI ACCWGGT 1 cut(s) 291
Csp6I GTAC 3 cut(s) 262, 517, 900
CviAII CATG 7 cut(s) 133, 170, 271, 377, 413, 680, 888
CviQI GTAC 3 cut(s) 262, 517, 900
DdeI CTNAG 7 cut(s) 24, 222, 315, 446, 557, 602, 918
DpnI GATC 4 cut(s) 323, 491, 507, 909
DpnII GATC 4 cut(s) 321, 489, 505, 907
DraIII CACNNNGTG 1 cut(s) 225
Eam1104I CTCTTC 1 cut(s) 663
EarI CTCTTC 1 cut(s) 663
Eco31I GGTCTC 1 cut(s) 754
EcoO109I RGGNCCY 1 cut(s) 190
EcoRI GAATTC 1 cut(s) 64
EcoRII CCWGG 2 cut(s) 291, 895
EcoT22I ATGCAT 1 cut(s) 724
FaeI CATG 7 cut(s) 136, 173, 274, 380, 416, 683, 891
FalI AAGNNNNNCTT 2 cut(s) 492, 524
FaqI GGGAC 1 cut(s) 193
FatI CATG 7 cut(s) 132, 169, 270, 376, 412, 679, 887
FblI GTMKAC 2 cut(s) 288, 964
Fnu4HI GCNGC 1 cut(s) 419
FokI GGATG 1 cut(s) 166
Fsp4HI GCNGC 1 cut(s) 419
FspBI CTAG 3 cut(s) 195, 774, 948
GluI GCNGC 1 cut(s) 419
GsuI CTGGAG 4 cut(s) 30, 356, 732, 795
HaeIII GGCC 2 cut(s) 191, 269
HapII CCGG 1 cut(s) 649
Hin1II CATG 7 cut(s) 136, 173, 274, 380, 416, 683, 891
HincII GTYRAC 1 cut(s) 289
HindII GTYRAC 1 cut(s) 289
HinfI GANTC 6 cut(s) 5, 356, 794, 817, 884, 929
HpaII CCGG 1 cut(s) 649
HphI GGTGA 1 cut(s) 430
Hpy166II GTNNAC 3 cut(s) 289, 390, 965
Hpy188I TCNGA 8 cut(s) 42, 70, 117, 361, 558, 605, 759, 919
Hpy8I GTNNAC 3 cut(s) 289, 390, 965
HpyAV CCTTC 1 cut(s) 517
HpyCH4III ACNGT 3 cut(s) 46, 432, 584
HpyCH4IV ACGT 1 cut(s) 478
HpyCH4V TGCA 7 cut(s) 157, 390, 406, 486, 572, 722, 803
HpyF10VI GCNNNNNNNGC 5 cut(s) 418, 779, 854, 863, 953
HpyF3I CTNAG 7 cut(s) 24, 222, 315, 446, 557, 602, 918
HpySE526I ACGT 1 cut(s) 478
Hsp92II CATG 7 cut(s) 136, 173, 274, 380, 416, 683, 891
Kzo9I GATC 4 cut(s) 321, 489, 505, 907
LguI GCTCTTC 1 cut(s) 663
LmnI GCTCC 3 cut(s) 532, 652, 814
Lsp1109I GCAGC 1 cut(s) 430
LweI GCATC 7 cut(s) 144, 202, 559, 709, 731, 760, 943
MabI ACCWGGT 1 cut(s) 291
MaeI CTAG 3 cut(s) 195, 774, 948
MaeII ACGT 1 cut(s) 478
MaeIII GTNAC 4 cut(s) 172, 712, 868, 975
MalI GATC 4 cut(s) 323, 491, 507, 909
MboI GATC 4 cut(s) 321, 489, 505, 907
MboII GAAGA 8 cut(s) 136, 137, 242, 340, 680, 779, 902, 917
MfeI CAATTG 1 cut(s) 804
MflI RGATCY 2 cut(s) 505, 907
MhlI GDGCHC 1 cut(s) 392
MluCI AATT 5 cut(s) 64, 109, 183, 465, 804
MlyI GAGTC 2 cut(s) 365, 878
Mph1103I ATGCAT 1 cut(s) 724
MroXI GAANNNNTTC 2 cut(s) 840, 909
MseI TTAA 2 cut(s) 186, 942
MslI CAYNNNNRTG 1 cut(s) 582
MspI CCGG 1 cut(s) 649
MspR9I CCNGG 3 cut(s) 293, 650, 897
MunI CAATTG 1 cut(s) 804
Mva1269I GAATGC 1 cut(s) 655
MvaI CCWGG 2 cut(s) 293, 897
MwoI GCNNNNNNNGC 5 cut(s) 418, 779, 854, 863, 953
NciI CCSGG 1 cut(s) 650
NdeII GATC 4 cut(s) 321, 489, 505, 907
NlaIII CATG 7 cut(s) 136, 173, 274, 380, 416, 683, 891
NlaIV GGNNCC 5 cut(s) 91, 106, 192, 783, 825
NsiI ATGCAT 1 cut(s) 724
NspI RCATGY 1 cut(s) 173
OliI CACNNNNGTG 1 cut(s) 582
PagI TCATGA 2 cut(s) 132, 376
PciSI GCTCTTC 1 cut(s) 663
PctI GAATGC 1 cut(s) 655
PdmI GAANNNNTTC 2 cut(s) 840, 909
PfeI GAWTC 4 cut(s) 5, 794, 817, 929
PflFI GACNNNGTC 1 cut(s) 284
PfoI TCCNGGA 1 cut(s) 648
PkrI GCNGC 1 cut(s) 420
PleI GAGTC 2 cut(s) 364, 878
PpsI GAGTC 2 cut(s) 364, 878
PshAI GACNNNNGTC 1 cut(s) 888
Psp6I CCWGG 2 cut(s) 291, 895
PspGI CCWGG 2 cut(s) 291, 895
PspN4I GGNNCC 5 cut(s) 91, 106, 192, 783, 825
PspPI GGNCC 1 cut(s) 190
PstNI CAGNNNCTG 1 cut(s) 833
PsuI RGATCY 2 cut(s) 505, 907
PsyI GACNNNGTC 1 cut(s) 284
RsaI GTAC 3 cut(s) 263, 518, 901
RsaNI GTAC 3 cut(s) 262, 517, 900
RseI CAYNNNNRTG 1 cut(s) 582
SalI GTCGAC 1 cut(s) 287
SapI GCTCTTC 1 cut(s) 663
SaqAI TTAA 2 cut(s) 186, 942
SatI GCNGC 1 cut(s) 419
Sau3AI GATC 4 cut(s) 321, 489, 505, 907
Sau96I GGNCC 1 cut(s) 190
SchI GAGTC 2 cut(s) 365, 878
ScrFI CCNGG 3 cut(s) 293, 650, 897
SduI GDGCHC 1 cut(s) 392
SexAI ACCWGGT 1 cut(s) 291
SfaNI GCATC 7 cut(s) 144, 202, 559, 709, 731, 760, 943
SmiMI CAYNNNNRTG 1 cut(s) 582
SmlI CTYRAG 1 cut(s) 536
SmoI CTYRAG 1 cut(s) 536
Sse9I AATT 5 cut(s) 64, 109, 183, 465, 804
SspMI CTAG 3 cut(s) 195, 774, 948
StyD4I CCNGG 3 cut(s) 291, 648, 895
TaaI ACNGT 3 cut(s) 46, 432, 584
TaiI ACGT 1 cut(s) 481
TaqI TCGA 2 cut(s) 148, 288
TaqII GACCGA 1 cut(s) 626
TasI AATT 5 cut(s) 64, 109, 183, 465, 804
TatI WGTACW 1 cut(s) 261
TfiI GAWTC 4 cut(s) 5, 794, 817, 929
Tru1I TTAA 2 cut(s) 186, 942
Tru9I TTAA 2 cut(s) 186, 942
TscAI CASTG 4 cut(s) 339, 397, 429, 589
TseI GCWGC 1 cut(s) 418
TspDTI ATGAA 6 cut(s) 69, 287, 355, 365, 536, 648
TspRI CASTG 4 cut(s) 339, 397, 429, 589
Tth111I GACNNNGTC 1 cut(s) 284
VneI GTGCAC 1 cut(s) 388
XapI RAATTY 1 cut(s) 64
XceI RCATGY 1 cut(s) 173
XmiI GTMKAC 2 cut(s) 288, 964
XmnI GAANNNNTTC 2 cut(s) 840, 909
XspI CTAG 3 cut(s) 195, 774, 948
Zsp2I ATGCAT 1 cut(s) 724
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.