FvH4_5g39631
RLK Family

cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000146
Physical Location & Seq
Forward (+)
29227505 .. 29231805
4301 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g39631.t1

Sequence Viewer

Length: 942 bp
ATGGTGGATCTGAACGTTATCGAAGGGAAGAAGGAAGAATCTGGTGAGGGGAGTTCTATGACCCGTGAAGTTGTAGTTGTTCAGAATGTTACAGATAATTCGAGTTTTGGCATCAAGCTTGATGGTAACAATTATCAGTTGTGGCAAAGGCTTATGAAGATCCATATCGAAGGAATGGGGAAGTGGGGTTACGTTGATGGTAGCACTCCAAGACCTACTGCAGGATCAAAGATCGATGAATGGGATACGGCGAATACTAATGTGATGGGGATTCTCCTGAAAGCTATGACTCCAGAGGTAATGGAGTTGTTTTCTAATTTTGATTCTCCTAAGGCAATCTGGGACTCTGTTGCTGCTACCTATTATGATGGGAATGACTTTGCTCGTGTCCATGAGTTGAATGTGAAAGCTTTCCAACTGACTCAAAGTGGACAGCCTGTGGCAACGTTTTATGCAAGTTTAAAGACCATATGGCAGGAGCTTGATCAGAGAAATCCGAATCCTATGACATGTGGAGCAGATATCACTACCTACAGGAATGAACAAGACAAGATGCGTGCCGAGCTTAAGGAGGAGGTTGCAGGCCGAGCTTCTGAAAGACTAAGACCATTTGTTGTTGCTCGCTTGGCACACTCGACACGTTTCGAGACCTCTCGGAGTTGCTGCTTGTATAACGAGATGATTTCCAGTAGTGTATCTTCTACTTCTTCTGAGCTAGTGTCGCCACATCGGTTAAGGCTAGGCCCCTCTTTGCATCTTCAAGGTAGAGGGTTGTGGGTGGTGGCTGAGCAGGTCCAGAATCTAGGATCCTGGTACGGCGACATTGCTGAGCTAGGATTAGGGGGTTTGGTCACCATTTTGGAAGCCTTCTCCTCTCTTCAACCTAACATCAATGCTTGGGTTGGGTTTGATATGCAACAGCTTCACCGGAATGACGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 Retrotran_gag_3 Retrotrans_gag gag_pre-integrs rve

Protein Analysis

314

Amino Acids

34.96

Weight (kDa)

5.18

Isoelectric Point (pI)

40.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotran_gag_3 PF14244 38 - 72 4.2e-08 gag-polypeptide of LTR copia-type
Retrotran_gag_2 PF14223 52 - 163 2.6e-06 gag-polypeptide of LTR copia-type
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000146)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19561 FvH4_1g25371 FvH4_1g25961 FvH4_2g00352 FvH4_2g00861 FvH4_2g04952 FvH4_2g05413 FvH4_2g07531 FvH4_2g08961 FvH4_2g25141 FvH4_2g27292 FvH4_3g18164 FvH4_3g19921 FvH4_3g43870 FvH4_4g14441 FvH4_4g15581 FvH4_4g15582 FvH4_4g16890 FvH4_4g19782 FvH4_4g33444 FvH4_4g33921 FvH4_5g22281 FvH4_5g22282 FvH4_5g32571 FvH4_5g37451 FvH4_5g39631 FvH4_6g04721 FvH4_6g11661 FvH4_6g20571 FvH4_6g32791 FvH4_7g23184
prunus_persica Prupe.1G090900_v2.0.a1 Prupe.1G241200_v2.0.a1 Prupe.1G534100_v2.0.a1 Prupe.3G076700_v2.0.a1 Prupe.4G150800_v2.0.a1 Prupe.4G181800_v2.0.a1 Prupe.4G240900_v2.0.a1 Prupe.4G247600_v2.0.a1 Prupe.6G115900_v2.0.a1 Prupe.6G327500_v2.0.a1 Prupe.8G071700_v2.0.a1
pyrus_communis pycom06g11480 pycom10g08330 pycom15g04320 pycom15g04350 pycom15g12620 pycom2874g00060
rosa_chinensis RchiOBHm_Chr1g0317241 RchiOBHm_Chr1g0341801 RchiOBHm_Chr1g0341931 RchiOBHm_Chr1g0359621 RchiOBHm_Chr2g0113741 RchiOBHm_Chr2g0117261 RchiOBHm_Chr2g0138831 RchiOBHm_Chr2g0141421 RchiOBHm_Chr2g0145281 RchiOBHm_Chr2g0152991 RchiOBHm_Chr3g0448001 RchiOBHm_Chr3g0450981 RchiOBHm_Chr3g0455161 RchiOBHm_Chr3g0457401 RchiOBHm_Chr3g0460001 RchiOBHm_Chr4g0387431 RchiOBHm_Chr4g0388741 RchiOBHm_Chr4g0444201 RchiOBHm_Chr4g0444261 RchiOBHm_Chr5g0010221 RchiOBHm_Chr6g0271721 RchiOBHm_Chr6g0274641 RchiOBHm_Chr6g0294691 RchiOBHm_Chr7g0237031 RchiOBHm_Chr7g0238461 RchiOBHm_Chr7g0239981
rosa_laevigata RLG00000009017
rosa_multiflora Rmu_co7986768.1_g000001 Rmu_co8302239.1_g000001 Rmu_co8485799.1_g000001 Rmu_sc0000061.1_g000013 Rmu_sc0000303.1_g000034 Rmu_sc0000303.1_g000035 Rmu_sc0000315.1_g000017 Rmu_sc0000349.1_g000003 Rmu_sc0000498.1_g000068 Rmu_sc0000508.1_g000014 Rmu_sc0000602.1_g000001 Rmu_sc0000633.1_g000002 Rmu_sc0000638.1_g000030 Rmu_sc0000756.1_g000016 Rmu_sc0000756.1_g000017 Rmu_sc0000785.1_g000037 Rmu_sc0000795.1_g000017 Rmu_sc0000796.1_g000030 Rmu_sc0000863.1_g000023 Rmu_sc0000920.1_g000021 Rmu_sc0000960.1_g000015 Rmu_sc0000982.1_g000102 Rmu_sc0001036.1_g000008 Rmu_sc0001036.1_g000009 Rmu_sc0001643.1_g000029 Rmu_sc0001687.1_g000026 Rmu_sc0001708.1_g000037 Rmu_sc0001768.1_g000015 Rmu_sc0001790.1_g000022 Rmu_sc0002180.1_g000007 Rmu_sc0002211.1_g000006 Rmu_sc0002270.1_g000010 Rmu_sc0002404.1_g000031 Rmu_sc0002858.1_g000016 Rmu_sc0003063.1_g000028 Rmu_sc0003730.1_g000017 Rmu_sc0004087.1_g000020 Rmu_sc0004618.1_g000048 Rmu_sc0004724.1_g000005 Rmu_sc0005167.1_g000036 Rmu_sc0005781.1_g000003 Rmu_sc0005878.1_g000012 Rmu_sc0006011.1_g000003 Rmu_sc0006343.1_g000011 Rmu_sc0006573.1_g000007 Rmu_sc0006599.1_g000007 Rmu_sc0006803.1_g000014 Rmu_sc0006836.1_g000002 Rmu_sc0006849.1_g000002 Rmu_sc0007109.1_g000009 Rmu_sc0009268.1_g000016 Rmu_sc0009618.1_g000017 Rmu_sc0009674.1_g000009 Rmu_sc0010244.1_g000009 Rmu_sc0011151.1_g000004 Rmu_sc0011356.1_g000001 Rmu_sc0011674.1_g000002 Rmu_sc0011778.1_g000016 Rmu_sc0012342.1_g000001 Rmu_sc0012467.1_g000002 Rmu_sc0012574.1_g000003 Rmu_sc0012913.1_g000005 Rmu_sc0013612.1_g000020 Rmu_sc0014824.1_g000001 Rmu_sc0017869.1_g000002 Rmu_sc0018085.1_g000003 Rmu_sc0024789.1_g000001 Rmu_sc0025106.1_g000013 Rmu_sc0034502.1_g000001 Rmu_sc0034547.1_g000001 Rmu_sc0035094.1_g000001 Rmu_sc0035095.1_g000001 Rmu_ssc0000100.1_g000027 Rmu_ssc0000244.1_g000005 Rmu_ssc0000371.1_g000029 Rmu_ssc0000381.1_g000004
rosa_roxburghii Rroxscaffold_1G00015480 Rroxscaffold_1G00023580 Rroxscaffold_1G00039340 Rroxscaffold_1G00047080 Rroxscaffold_2G00095820 Rroxscaffold_2G00125200 Rroxscaffold_2G00128330 Rroxscaffold_3G00270860
rosa_rugosa Rorug06G0120700
rosa_samantha Rh3BG173100 Rh6AG089600 Rh7AG488100 Rh7DG389600
rosa_wichuraiana Rw0G001150 Rw0G001990 Rw0G002010 Rw0G019540 Rw1G013730 Rw1G024110 Rw1G029190 Rw2G018850 Rw2G021090 Rw2G022260 Rw2G023180 Rw2G027280 Rw3G010190 Rw3G020660 Rw3G029380 Rw4G002390 Rw4G002690 Rw4G033260 Rw5G011960 Rw5G023110 Rw5G025250 Rw5G028950 Rw5G033740 Rw5G033950 Rw5G037900 Rw6G009640 Rw6G011620 Rw6G027720 Rw7G010680 Rw7G042070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 781
AclI AACGTT 2 cut(s) 15, 446
AclWI GGATC 5 cut(s) 15, 154, 232, 801, 814
AfaI GTAC 1 cut(s) 815
AfiI CCNNNNNNNGG 1 cut(s) 221
AflII CTTAAG 1 cut(s) 566
AflIII ACRYGT 2 cut(s) 509, 638
AgsI TTSAA 3 cut(s) 400, 761, 881
AjnI CCWGG 1 cut(s) 809
AluBI AGCT 9 cut(s) 118, 284, 410, 481, 565, 590, 715, 832, 922
AluI AGCT 9 cut(s) 118, 284, 410, 481, 565, 590, 715, 832, 922
Alw26I GTCTC 1 cut(s) 641
AlwI GGATC 5 cut(s) 15, 154, 232, 801, 814
AoxI GGCC 2 cut(s) 583, 742
ApeKI GCWGC 2 cut(s) 353, 663
Asp700I GAANNNNTTC 1 cut(s) 410
AspS9I GGNCC 2 cut(s) 743, 793
AsuHPI GGTGA 3 cut(s) 56, 844, 917
AvaII GGWCC 1 cut(s) 793
AxyI CCTNAGG 1 cut(s) 330
BamHI GGATCC 1 cut(s) 806
BarI GAAGNNNNNNTAC 4 cut(s) 173, 205, 682, 714
BauI CACGAG 1 cut(s) 384
BbvI GCAGC 2 cut(s) 340, 650
BccI CCATC 4 cut(s) 116, 191, 259, 362
BceAI ACGGC 2 cut(s) 264, 832
BciT130I CCWGG 1 cut(s) 811
BciVI GTATCC 1 cut(s) 238
BclI TGATCA 1 cut(s) 484
BcoDI GTCTC 1 cut(s) 641
BfaI CTAG 4 cut(s) 716, 740, 803, 833
BfmI CTRYAG 2 cut(s) 219, 532
BfrI CTTAAG 1 cut(s) 566
BfuAI ACCTGC 1 cut(s) 781
BfuI GTATCC 1 cut(s) 238
BisI GCNGC 2 cut(s) 354, 664
BlpI GCTNAGC 2 cut(s) 786, 828
BlsI GCNGC 2 cut(s) 355, 665
Bme1390I CCNGG 1 cut(s) 811
Bme18I GGWCC 1 cut(s) 793
BmgT120I GGNCC 2 cut(s) 743, 793
BmiI GGNNCC 2 cut(s) 745, 808
BmrFI CCNGG 1 cut(s) 811
BmsI GCATC 3 cut(s) 120, 543, 763
BpmI CTGGAG 1 cut(s) 276
Bpu1102I GCTNAGC 2 cut(s) 786, 828
Bsa29I ATCGAT 1 cut(s) 234
BsaBI GATNNNNATC 1 cut(s) 164
BsaI GGTCTC 1 cut(s) 641
BsaWI WCCGGW 1 cut(s) 927
Bsc4I CCNNNNNNNGG 1 cut(s) 221
Bse1I ACTGG 1 cut(s) 687
Bse21I CCTNAGG 1 cut(s) 330
Bse3DI GCAATG 1 cut(s) 822
Bse8I GATNNNNATC 1 cut(s) 164
BseBI CCWGG 1 cut(s) 811
BseCI ATCGAT 1 cut(s) 234
BseJI GATNNNNATC 1 cut(s) 164
BseLI CCNNNNNNNGG 1 cut(s) 221
BseMI GCAATG 1 cut(s) 822
BseMII CTCAG 3 cut(s) 702, 777, 819
BseNI ACTGG 1 cut(s) 687
BseRI GAGGAG 2 cut(s) 587, 862
BseXI GCAGC 2 cut(s) 340, 650
BshFI GGCC 2 cut(s) 585, 744
BshVI ATCGAT 1 cut(s) 234
BsiSI CCGG 1 cut(s) 928
BslFI GGGAC 1 cut(s) 356
BslI CCNNNNNNNGG 1 cut(s) 221
BsmAI GTCTC 1 cut(s) 641
BsmFI GGGAC 1 cut(s) 356
BsnI GGCC 2 cut(s) 585, 744
Bso31I GGTCTC 1 cut(s) 641
Bsp143I GATC 6 cut(s) 7, 159, 224, 231, 484, 806
Bsp1720I GCTNAGC 2 cut(s) 786, 828
BspANI GGCC 2 cut(s) 585, 744
BspCNI CTCAG 3 cut(s) 703, 778, 820
BspDI ATCGAT 1 cut(s) 234
BspLI GGNNCC 2 cut(s) 745, 808
BspMAI CTGCAG 1 cut(s) 223
BspMI ACCTGC 1 cut(s) 781
BspPI GGATC 5 cut(s) 15, 154, 232, 801, 814
BspTI CTTAAG 1 cut(s) 566
BspTNI GGTCTC 1 cut(s) 641
BsrDI GCAATG 1 cut(s) 822
BsrI ACTGG 1 cut(s) 687
BssMI GATC 6 cut(s) 7, 159, 224, 231, 484, 806
BssSI CACGAG 1 cut(s) 384
Bst2BI CACGAG 1 cut(s) 384
Bst2UI CCWGG 1 cut(s) 811
Bst6I CTCTTC 1 cut(s) 882
BstAFI CTTAAG 1 cut(s) 566
BstC8I GCNNGC 3 cut(s) 558, 583, 622
BstDEI CTNAG 5 cut(s) 330, 602, 711, 786, 828
BstEII GGTNACC 1 cut(s) 850
BstENI CCTNNNNNAGG 1 cut(s) 219
BstKTI GATC 6 cut(s) 10, 162, 227, 234, 487, 809
BstMAI GTCTC 1 cut(s) 641
BstMBI GATC 6 cut(s) 7, 159, 224, 231, 484, 806
BstMWI GCNNNNNNNGC 4 cut(s) 562, 587, 626, 721
BstNI CCWGG 1 cut(s) 811
BstNSI RCATGY 1 cut(s) 513
BstPI GGTNACC 1 cut(s) 850
BstSCI CCNGG 1 cut(s) 809
BstSFI CTRYAG 2 cut(s) 219, 532
BstV1I GCAGC 2 cut(s) 340, 650
BstX2I RGATCY 3 cut(s) 7, 159, 806
BstYI RGATCY 3 cut(s) 7, 159, 806
Bsu15I ATCGAT 1 cut(s) 234
Bsu36I CCTNAGG 1 cut(s) 330
BsuI GTATCC 1 cut(s) 238
BsuRI GGCC 2 cut(s) 585, 744
BsuTUI ATCGAT 1 cut(s) 234
BveI ACCTGC 1 cut(s) 781
Cac8I GCNNGC 3 cut(s) 558, 583, 622
Cfr13I GGNCC 2 cut(s) 743, 793
ClaI ATCGAT 1 cut(s) 234
Csp6I GTAC 1 cut(s) 814
CviAII CATG 2 cut(s) 392, 510
CviQI GTAC 1 cut(s) 814
DdeI CTNAG 5 cut(s) 330, 602, 711, 786, 828
DpnI GATC 6 cut(s) 9, 161, 226, 233, 486, 808
DpnII GATC 6 cut(s) 7, 159, 224, 231, 484, 806
DraI TTTAAA 1 cut(s) 462
Eam1104I CTCTTC 1 cut(s) 882
EarI CTCTTC 1 cut(s) 882
Eco31I GGTCTC 1 cut(s) 641
Eco32I GATATC 1 cut(s) 523
Eco47I GGWCC 1 cut(s) 793
Eco81I CCTNAGG 1 cut(s) 330
Eco91I GGTNACC 1 cut(s) 850
EcoNI CCTNNNNNAGG 1 cut(s) 219
EcoO109I RGGNCCY 1 cut(s) 743
EcoO65I GGTNACC 1 cut(s) 850
EcoRII CCWGG 1 cut(s) 809
EcoRV GATATC 1 cut(s) 523
FaeI CATG 2 cut(s) 395, 513
FaqI GGGAC 1 cut(s) 356
FatI CATG 2 cut(s) 391, 509
FauNDI CATATG 1 cut(s) 470
FbaI TGATCA 1 cut(s) 484
Fnu4HI GCNGC 2 cut(s) 354, 664
Fsp4HI GCNGC 2 cut(s) 354, 664
FspBI CTAG 4 cut(s) 716, 740, 803, 833
GluI GCNGC 2 cut(s) 354, 664
GsuI CTGGAG 1 cut(s) 276
HaeIII GGCC 2 cut(s) 585, 744
HapII CCGG 1 cut(s) 928
Hin1II CATG 2 cut(s) 395, 513
HindIII AAGCTT 2 cut(s) 116, 408
HinfI GANTC 8 cut(s) 38, 271, 289, 323, 344, 421, 499, 799
HpaII CCGG 1 cut(s) 928
HphI GGTGA 3 cut(s) 56, 844, 917
Hpy166II GTNNAC 1 cut(s) 431
Hpy188I TCNGA 7 cut(s) 12, 84, 489, 498, 595, 657, 712
Hpy188III TCNNGA 4 cut(s) 277, 293, 646, 796
Hpy8I GTNNAC 1 cut(s) 431
HpyAV CCTTC 4 cut(s) 17, 25, 164, 877
HpyCH4IV ACGT 4 cut(s) 15, 192, 446, 640
HpyCH4V TGCA 5 cut(s) 221, 455, 581, 754, 916
HpyF10VI GCNNNNNNNGC 4 cut(s) 562, 587, 626, 721
HpyF3I CTNAG 5 cut(s) 330, 602, 711, 786, 828
HpySE526I ACGT 4 cut(s) 15, 192, 446, 640
Hsp92II CATG 2 cut(s) 395, 513
Ksp22I TGATCA 1 cut(s) 484
Kzo9I GATC 6 cut(s) 7, 159, 224, 231, 484, 806
LmnI GCTCC 2 cut(s) 478, 515
Lsp1109I GCAGC 2 cut(s) 340, 650
LweI GCATC 3 cut(s) 120, 543, 763
MaeI CTAG 4 cut(s) 716, 740, 803, 833
MaeII ACGT 4 cut(s) 15, 192, 446, 640
MaeIII GTNAC 4 cut(s) 88, 125, 188, 850
MalI GATC 6 cut(s) 9, 161, 226, 233, 486, 808
MboI GATC 6 cut(s) 7, 159, 224, 231, 484, 806
MboII GAAGA 7 cut(s) 40, 47, 169, 690, 699, 749, 869
MflI RGATCY 3 cut(s) 7, 159, 806
MluCI AATT 3 cut(s) 97, 130, 316
MlyI GAGTC 3 cut(s) 283, 338, 415
MmeI TCCRAC 1 cut(s) 439
MnlI CCTC 8 cut(s) 40, 289, 565, 568, 661, 757, 761, 883
MroXI GAANNNNTTC 1 cut(s) 410
MseI TTAA 4 cut(s) 461, 567, 734, 940
MslI CAYNNNNRTG 1 cut(s) 930
MspCI CTTAAG 1 cut(s) 566
MspI CCGG 1 cut(s) 928
MspR9I CCNGG 1 cut(s) 811
MvaI CCWGG 1 cut(s) 811
MwoI GCNNNNNNNGC 4 cut(s) 562, 587, 626, 721
NdeI CATATG 1 cut(s) 470
NdeII GATC 6 cut(s) 7, 159, 224, 231, 484, 806
NlaIII CATG 2 cut(s) 395, 513
NlaIV GGNNCC 2 cut(s) 745, 808
NmeAIII GCCGAG 2 cut(s) 586, 611
NmuCI GTSAC 1 cut(s) 850
NspI RCATGY 1 cut(s) 513
PciI ACATGT 1 cut(s) 509
PdmI GAANNNNTTC 1 cut(s) 410
PfeI GAWTC 5 cut(s) 38, 271, 323, 499, 799
PkrI GCNGC 2 cut(s) 355, 665
PleI GAGTC 3 cut(s) 283, 338, 415
PpsI GAGTC 3 cut(s) 283, 338, 415
PscI ACATGT 1 cut(s) 509
Psp1406I AACGTT 2 cut(s) 15, 446
Psp6I CCWGG 1 cut(s) 809
PspEI GGTNACC 1 cut(s) 850
PspGI CCWGG 1 cut(s) 809
PspN4I GGNNCC 2 cut(s) 745, 808
PspPI GGNCC 2 cut(s) 743, 793
PstI CTGCAG 1 cut(s) 223
PsuI RGATCY 3 cut(s) 7, 159, 806
RsaI GTAC 1 cut(s) 815
RsaNI GTAC 1 cut(s) 814
RseI CAYNNNNRTG 1 cut(s) 930
SaqAI TTAA 4 cut(s) 461, 567, 734, 940
SatI GCNGC 2 cut(s) 354, 664
Sau3AI GATC 6 cut(s) 7, 159, 224, 231, 484, 806
Sau96I GGNCC 2 cut(s) 743, 793
SchI GAGTC 3 cut(s) 283, 338, 415
ScrFI CCNGG 1 cut(s) 811
SfaNI GCATC 3 cut(s) 120, 543, 763
SfcI CTRYAG 2 cut(s) 219, 532
SinI GGWCC 1 cut(s) 793
SmiMI CAYNNNNRTG 1 cut(s) 930
SmlI CTYRAG 1 cut(s) 566
SmoI CTYRAG 1 cut(s) 566
Sse9I AATT 3 cut(s) 97, 130, 316
SspMI CTAG 4 cut(s) 716, 740, 803, 833
StyD4I CCNGG 1 cut(s) 809
TaiI ACGT 4 cut(s) 18, 195, 449, 643
TaqI TCGA 6 cut(s) 21, 101, 168, 234, 635, 645
TasI AATT 3 cut(s) 97, 130, 316
TfiI GAWTC 5 cut(s) 38, 271, 323, 499, 799
Tru1I TTAA 4 cut(s) 461, 567, 734, 940
Tru9I TTAA 4 cut(s) 461, 567, 734, 940
TseFI GTSAC 1 cut(s) 850
TseI GCWGC 2 cut(s) 353, 663
Tsp45I GTSAC 1 cut(s) 850
TspDTI ATGAA 3 cut(s) 170, 252, 555
Vha464I CTTAAG 1 cut(s) 566
VpaK11BI GGWCC 1 cut(s) 793
XagI CCTNNNNNAGG 1 cut(s) 219
XceI RCATGY 1 cut(s) 513
XmnI GAANNNNTTC 1 cut(s) 410
XspI CTAG 4 cut(s) 716, 740, 803, 833
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.