Rw5G025250

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000146
Physical Location & Seq
Forward (+)
35916152 .. 35921315
5164 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G025250.1

Sequence Viewer

Length: 1653 bp
ATGGTTCTCGATGAAGTACTTGCTGATTTATCCCACACATCAGCAACCAACTCTGAATCTTCTTCTCAACATAAAGGGCGATCGTCATCCAATGATCATGGCCCTATTGCTCCAGAAAAATTGGATAGAACCAATTTTATTTATTGGTCTAAACATGCCAAGTTGAACATCACCAGCCAAGGTAAGCTTGGCTATCTCACCGGCAAGAAGAAAGCACCATCTGAAGAAGAAGATGAGGAGGGTTATGAAATTTGGGTTGAAGAAGACTGTCTTGTTCAAGCATGGCTCCTCAACTCCATACACAAGGATGTTAGAGGAAATTATGAAAGCTTGGAGATAGCTAAAGAAATTTGGGATGCTGTCAAAACTACTTTTTCAGTAGCTCAAGATGATACAAGAAACTATGAGCTGCATATTGCTTCTGTTTCCACTAAACAGAATGGAGCTCATCTTCATGGCTATTACAACAATATGAAGAAAATAGGGCAGGAGCTTGACATATTGGATCCTCTCCAGCTCAAGGATGCAGAGTCTATTGCTTACATGGCAGAAAAGGTTAGTAAGCACTGTGTCTACTTCTTCTTGGCAGGTCTTGATCCATACCTCGATGGTGTCAGAAGTAGAATTCTAAACACAAAGCCACTACCCTCTATTGCAGAGACTTATTCTCAAGTCAGATCTGCCATGCACACTCAATACCGGCCACCACGTGTTTGGAATTCTCAGTCTCCGCCATCTGCCTCCTCTTGGAAAGCACCCACTACAAGACCTATGAAATGCACTCATTGTAAGTCTCAGAAGCATACTATGGATACTTGTTTTCAATTGAAAGGTTATCTAGAATGGTGGCAGGAGCATGTTGCCCGTCGTAAGGAAATCCAAGAGAAAGAGGCAAAAGGATATCTGCCCAAAGGTAAACATAAGGTGGGCCTTCATATTACCTCATCAGCTCTGGAAGAACCAACTGAAGGTGATTACACTAGTGGCCCTTGTGATGCCCCGGAAATTTGTATTTATTTTTTGAGGATTTTTCGGAATCTAAATTATGGTTATTGGACTGTTTCGTGGCTCACCTTTCATGCCATGATCCATACCCAGTTTGTAACTCCCATTAAAGTTTTCTGCTCAGATAATGGTGGAGAATATGTCACACATACCTTCCGCCAATATCTCAACACTCATGGAATCATACACCAGACCACCTGTCATCATACACCTCAGCAAAATGGTGTGGAGGAACGCAAGAACCGCCACATTCTTGAAAATTTCTGGGGTGATGCCATCCATACTACTGCCTACCTCATAAATCAACTTCCCACACAAGCTCTCCAAACCTCTCTTGGGGAAGATAGTCCACCACCAAAAACATCTATCCAAGCCTTGTCCAAACACTTACCTTTACCACCTACCCATGCCCTTAATCCAAAGATTTTTGGTTGTGTATCTTATGTACATATCCATCGAACCAGAGGACTAAGTTGGAGGCTTGTGTTGAAAAGTGTGTCTTCATTGGTTATGCTTATACTCAAAAGGGATATTGGTGCTTTAATCCCATCACTAAACGAACATATGTTACTATGGATGTCACGTTCTGTGAACATGATCTCTATGTTGCCCCGAAGACTCCTCTTCAGGGGGAGAATCGTGATGTAG

Protein Analysis

550

Amino Acids

62.82

Weight (kDa)

8.6

Isoelectric Point (pI)

44.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000146)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19561 FvH4_1g25371 FvH4_1g25961 FvH4_2g00352 FvH4_2g00861 FvH4_2g04952 FvH4_2g05413 FvH4_2g07531 FvH4_2g08961 FvH4_2g25141 FvH4_2g27292 FvH4_3g18164 FvH4_3g19921 FvH4_3g43870 FvH4_4g14441 FvH4_4g15581 FvH4_4g15582 FvH4_4g16890 FvH4_4g19782 FvH4_4g33444 FvH4_4g33921 FvH4_5g22281 FvH4_5g22282 FvH4_5g32571 FvH4_5g37451 FvH4_5g39631 FvH4_6g04721 FvH4_6g11661 FvH4_6g20571 FvH4_6g32791 FvH4_7g23184
prunus_persica Prupe.1G090900_v2.0.a1 Prupe.1G241200_v2.0.a1 Prupe.1G534100_v2.0.a1 Prupe.3G076700_v2.0.a1 Prupe.4G150800_v2.0.a1 Prupe.4G181800_v2.0.a1 Prupe.4G240900_v2.0.a1 Prupe.4G247600_v2.0.a1 Prupe.6G115900_v2.0.a1 Prupe.6G327500_v2.0.a1 Prupe.8G071700_v2.0.a1
pyrus_communis pycom06g11480 pycom10g08330 pycom15g04320 pycom15g04350 pycom15g12620 pycom2874g00060
rosa_chinensis RchiOBHm_Chr1g0317241 RchiOBHm_Chr1g0341801 RchiOBHm_Chr1g0341931 RchiOBHm_Chr1g0359621 RchiOBHm_Chr2g0113741 RchiOBHm_Chr2g0117261 RchiOBHm_Chr2g0138831 RchiOBHm_Chr2g0141421 RchiOBHm_Chr2g0145281 RchiOBHm_Chr2g0152991 RchiOBHm_Chr3g0448001 RchiOBHm_Chr3g0450981 RchiOBHm_Chr3g0455161 RchiOBHm_Chr3g0457401 RchiOBHm_Chr3g0460001 RchiOBHm_Chr4g0387431 RchiOBHm_Chr4g0388741 RchiOBHm_Chr4g0444201 RchiOBHm_Chr4g0444261 RchiOBHm_Chr5g0010221 RchiOBHm_Chr6g0271721 RchiOBHm_Chr6g0274641 RchiOBHm_Chr6g0294691 RchiOBHm_Chr7g0237031 RchiOBHm_Chr7g0238461 RchiOBHm_Chr7g0239981
rosa_laevigata RLG00000009017
rosa_multiflora Rmu_co7986768.1_g000001 Rmu_co8302239.1_g000001 Rmu_co8485799.1_g000001 Rmu_sc0000061.1_g000013 Rmu_sc0000303.1_g000034 Rmu_sc0000303.1_g000035 Rmu_sc0000315.1_g000017 Rmu_sc0000349.1_g000003 Rmu_sc0000498.1_g000068 Rmu_sc0000508.1_g000014 Rmu_sc0000602.1_g000001 Rmu_sc0000633.1_g000002 Rmu_sc0000638.1_g000030 Rmu_sc0000756.1_g000016 Rmu_sc0000756.1_g000017 Rmu_sc0000785.1_g000037 Rmu_sc0000795.1_g000017 Rmu_sc0000796.1_g000030 Rmu_sc0000863.1_g000023 Rmu_sc0000920.1_g000021 Rmu_sc0000960.1_g000015 Rmu_sc0000982.1_g000102 Rmu_sc0001036.1_g000008 Rmu_sc0001036.1_g000009 Rmu_sc0001643.1_g000029 Rmu_sc0001687.1_g000026 Rmu_sc0001708.1_g000037 Rmu_sc0001768.1_g000015 Rmu_sc0001790.1_g000022 Rmu_sc0002180.1_g000007 Rmu_sc0002211.1_g000006 Rmu_sc0002270.1_g000010 Rmu_sc0002404.1_g000031 Rmu_sc0002858.1_g000016 Rmu_sc0003063.1_g000028 Rmu_sc0003730.1_g000017 Rmu_sc0004087.1_g000020 Rmu_sc0004618.1_g000048 Rmu_sc0004724.1_g000005 Rmu_sc0005167.1_g000036 Rmu_sc0005781.1_g000003 Rmu_sc0005878.1_g000012 Rmu_sc0006011.1_g000003 Rmu_sc0006343.1_g000011 Rmu_sc0006573.1_g000007 Rmu_sc0006599.1_g000007 Rmu_sc0006803.1_g000014 Rmu_sc0006836.1_g000002 Rmu_sc0006849.1_g000002 Rmu_sc0007109.1_g000009 Rmu_sc0009268.1_g000016 Rmu_sc0009618.1_g000017 Rmu_sc0009674.1_g000009 Rmu_sc0010244.1_g000009 Rmu_sc0011151.1_g000004 Rmu_sc0011356.1_g000001 Rmu_sc0011674.1_g000002 Rmu_sc0011778.1_g000016 Rmu_sc0012342.1_g000001 Rmu_sc0012467.1_g000002 Rmu_sc0012574.1_g000003 Rmu_sc0012913.1_g000005 Rmu_sc0013612.1_g000020 Rmu_sc0014824.1_g000001 Rmu_sc0017869.1_g000002 Rmu_sc0018085.1_g000003 Rmu_sc0024789.1_g000001 Rmu_sc0025106.1_g000013 Rmu_sc0034502.1_g000001 Rmu_sc0034547.1_g000001 Rmu_sc0035094.1_g000001 Rmu_sc0035095.1_g000001 Rmu_ssc0000100.1_g000027 Rmu_ssc0000244.1_g000005 Rmu_ssc0000371.1_g000029 Rmu_ssc0000381.1_g000004
rosa_roxburghii Rroxscaffold_1G00015480 Rroxscaffold_1G00023580 Rroxscaffold_1G00039340 Rroxscaffold_1G00047080 Rroxscaffold_2G00095820 Rroxscaffold_2G00125200 Rroxscaffold_2G00128330 Rroxscaffold_3G00270860
rosa_rugosa Rorug06G0120700
rosa_samantha Rh3BG173100 Rh6AG089600 Rh7AG488100 Rh7DG389600
rosa_wichuraiana Rw0G001150 Rw0G001990 Rw0G002010 Rw0G019540 Rw1G013730 Rw1G024110 Rw1G029190 Rw2G018850 Rw2G021090 Rw2G022260 Rw2G023180 Rw2G027280 Rw3G010190 Rw3G020660 Rw3G029380 Rw4G002390 Rw4G002690 Rw4G033260 Rw5G011960 Rw5G023110 Rw5G025250 Rw5G028950 Rw5G033740 Rw5G033950 Rw5G037900 Rw6G009640 Rw6G011620 Rw6G027720 Rw7G010680 Rw7G042070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 578
AccI GTMKAC 1 cut(s) 573
AciI CCGC 3 cut(s) 731, 1162, 1249
AclWI GGATC 4 cut(s) 500, 513, 590, 1081
AcoI YGGCCR 1 cut(s) 701
AcsI RAATTY 6 cut(s) 249, 348, 624, 718, 1005, 1264
AcuI CTGAAG 3 cut(s) 243, 987, 1615
AcvI CACGTG 1 cut(s) 710
AdeI CACNNNGTG 1 cut(s) 710
AfaI GTAC 2 cut(s) 18, 1452
AfiI CCNNNNNNNGG 6 cut(s) 520, 747, 871, 968, 1341, 1633
AflIII ACRYGT 1 cut(s) 709
AgsI TTSAA 7 cut(s) 166, 260, 278, 824, 829, 1262, 1495
AhdI GACNNNNNGTC 1 cut(s) 1203
AhlI ACTAGT 1 cut(s) 980
AloI GAACNNNNNNTCC 2 cut(s) 1573, 1605
Alw21I GWGCWC 1 cut(s) 448
Alw26I GTCTC 3 cut(s) 653, 732, 798
AlwI GGATC 4 cut(s) 500, 513, 590, 1081
AoxI GGCC 4 cut(s) 100, 701, 928, 985
ApeKI GCWGC 1 cut(s) 409
ApoI RAATTY 6 cut(s) 249, 348, 624, 718, 1005, 1264
AspS9I GGNCC 3 cut(s) 101, 928, 986
AsuC2I CCSGG 1 cut(s) 1001
AsuHPI GGTGA 5 cut(s) 163, 190, 983, 1063, 1286
BamHI GGATCC 1 cut(s) 505
BanII GRGCYC 1 cut(s) 448
BbrPI CACGTG 1 cut(s) 710
BbsI GAAGAC 3 cut(s) 270, 1497, 1627
Bbv12I GWGCWC 1 cut(s) 448
BbvCI CCTCAGC 1 cut(s) 1218
BbvI GCAGC 1 cut(s) 396
BccI CCATC 6 cut(s) 226, 602, 742, 1289, 1467, 1561
BciVI GTATCC 1 cut(s) 805
BclI TGATCA 1 cut(s) 94
BcnI CCSGG 1 cut(s) 1001
BcoDI GTCTC 3 cut(s) 653, 732, 798
BcuI ACTAGT 1 cut(s) 980
BfaI CTAG 2 cut(s) 839, 981
BfuAI ACCTGC 1 cut(s) 578
BfuI GTATCC 1 cut(s) 805
BglII AGATCT 1 cut(s) 677
BisI GCNGC 1 cut(s) 410
BlsI GCNGC 1 cut(s) 411
BmcAI AGTACT 1 cut(s) 18
Bme1390I CCNGG 1 cut(s) 1001
BmeRI GACNNNNNGTC 1 cut(s) 1203
BmgT120I GGNCC 3 cut(s) 101, 928, 986
BmiI GGNNCC 2 cut(s) 287, 507
BmrFI CCNGG 1 cut(s) 1001
BmrI ACTGGG 1 cut(s) 1090
BmsI GCATC 4 cut(s) 346, 514, 985, 1267
BmuI ACTGGG 1 cut(s) 1090
BpiI GAAGAC 3 cut(s) 270, 1497, 1627
BpmI CTGGAG 2 cut(s) 96, 497
Bpu10I CCTNAGC 1 cut(s) 1218
BpuEI CTTGAG 3 cut(s) 369, 503, 654
BpuMI CCSGG 1 cut(s) 1001
BsaAI YACGTR 1 cut(s) 710
BsaBI GATNNNNATC 1 cut(s) 85
BsaJI CCNNGG 2 cut(s) 178, 999
BsaXI ACNNNNNCTCC 4 cut(s) 1131, 1161, 1311, 1341
Bsc4I CCNNNNNNNGG 6 cut(s) 520, 747, 871, 968, 1341, 1633
Bse118I RCCGGY 2 cut(s) 200, 699
Bse1I ACTGG 1 cut(s) 1096
Bse8I GATNNNNATC 1 cut(s) 85
BseDI CCNNGG 2 cut(s) 178, 999
BseGI GGATG 6 cut(s) 86, 313, 361, 529, 1281, 1587
BseJI GATNNNNATC 1 cut(s) 85
BseLI CCNNNNNNNGG 6 cut(s) 520, 747, 871, 968, 1341, 1633
BseMII CTCAG 4 cut(s) 737, 809, 1140, 1232
BseNI ACTGG 1 cut(s) 1096
BseRI GAGGAG 4 cut(s) 251, 278, 733, 1616
BseXI GCAGC 1 cut(s) 396
Bsh1285I CGRYCG 1 cut(s) 83
BshFI GGCC 4 cut(s) 102, 703, 930, 987
BsiEI CGRYCG 1 cut(s) 83
BsiHKAI GWGCWC 1 cut(s) 448
BsiSI CCGG 3 cut(s) 201, 700, 1001
BslI CCNNNNNNNGG 6 cut(s) 520, 747, 871, 968, 1341, 1633
BsmAI GTCTC 3 cut(s) 653, 732, 798
BsnI GGCC 4 cut(s) 102, 703, 930, 987
Bsp1286I GDGCHC 1 cut(s) 448
Bsp1407I TGTACA 1 cut(s) 1450
Bsp143I GATC 7 cut(s) 80, 94, 505, 595, 677, 1086, 1602
BspACI CCGC 3 cut(s) 731, 1162, 1249
BspANI GGCC 4 cut(s) 102, 703, 930, 987
BspCNI CTCAG 4 cut(s) 736, 808, 1139, 1231
BspLI GGNNCC 2 cut(s) 287, 507
BspMI ACCTGC 1 cut(s) 578
BspPI GGATC 4 cut(s) 500, 513, 590, 1081
BsrFI RCCGGY 2 cut(s) 200, 699
BsrGI TGTACA 1 cut(s) 1450
BsrI ACTGG 1 cut(s) 1096
BssAI RCCGGY 2 cut(s) 200, 699
BssECI CCNNGG 2 cut(s) 178, 999
BssMI GATC 7 cut(s) 80, 94, 505, 595, 677, 1086, 1602
BssT1I CCWWGG 1 cut(s) 178
Bst4CI ACNGT 3 cut(s) 269, 569, 1060
Bst6I CTCTTC 1 cut(s) 1634
BstAUI TGTACA 1 cut(s) 1450
BstBAI YACGTR 1 cut(s) 710
BstDEI CTNAG 5 cut(s) 723, 795, 1126, 1218, 1475
BstF5I GGATG 6 cut(s) 86, 313, 361, 529, 1281, 1587
BstKTI GATC 7 cut(s) 83, 97, 508, 598, 680, 1089, 1605
BstMAI GTCTC 3 cut(s) 653, 732, 798
BstMBI GATC 7 cut(s) 80, 94, 505, 595, 677, 1086, 1602
BstMCI CGRYCG 1 cut(s) 83
BstMWI GCNNNNNNNGC 2 cut(s) 545, 1248
BstNSI RCATGY 2 cut(s) 158, 860
BstSCI CCNGG 1 cut(s) 999
BstV1I GCAGC 1 cut(s) 396
BstV2I GAAGAC 3 cut(s) 270, 1497, 1627
BstX2I RGATCY 2 cut(s) 505, 677
BstXI CCANNNNNNTGG 1 cut(s) 714
BstYI RGATCY 2 cut(s) 505, 677
BsuI GTATCC 1 cut(s) 805
BsuRI GGCC 4 cut(s) 102, 703, 930, 987
BtsCI GGATG 6 cut(s) 86, 313, 361, 529, 1281, 1587
BtsIMutI CAGTG 1 cut(s) 565
BveI ACCTGC 1 cut(s) 578
Cfr10I RCCGGY 2 cut(s) 200, 699
Cfr13I GGNCC 3 cut(s) 101, 928, 986
Csp6I GTAC 2 cut(s) 17, 1451
CviQI GTAC 2 cut(s) 17, 1451
DdeI CTNAG 5 cut(s) 723, 795, 1126, 1218, 1475
DpnI GATC 7 cut(s) 82, 96, 507, 597, 679, 1088, 1604
DpnII GATC 7 cut(s) 80, 94, 505, 595, 677, 1086, 1602
DraIII CACNNNGTG 1 cut(s) 710
DriI GACNNNNNGTC 1 cut(s) 1203
EaeI YGGCCR 1 cut(s) 701
Eam1104I CTCTTC 1 cut(s) 1634
Eam1105I GACNNNNNGTC 1 cut(s) 1203
EarI CTCTTC 1 cut(s) 1634
EciI GGCGGA 2 cut(s) 720, 1151
Ecl136II GAGCTC 1 cut(s) 446
Eco130I CCWWGG 1 cut(s) 178
Eco24I GRGCYC 1 cut(s) 448
Eco32I GATATC 1 cut(s) 902
Eco53kI GAGCTC 1 cut(s) 446
Eco57I CTGAAG 3 cut(s) 243, 987, 1615
Eco72I CACGTG 1 cut(s) 710
EcoICRI GAGCTC 1 cut(s) 446
EcoRI GAATTC 2 cut(s) 624, 718
EcoRV GATATC 1 cut(s) 902
EcoT14I CCWWGG 1 cut(s) 178
EcoT38I GRGCYC 1 cut(s) 448
ErhI CCWWGG 1 cut(s) 178
FalI AAGNNNNNCTT 6 cut(s) 171, 203, 255, 287, 1489, 1521
FauNDI CATATG 1 cut(s) 1569
FbaI TGATCA 1 cut(s) 94
FblI GTMKAC 1 cut(s) 573
Fnu4HI GCNGC 1 cut(s) 410
FokI GGATG 6 cut(s) 73, 320, 368, 536, 1268, 1594
FriOI GRGCYC 1 cut(s) 448
Fsp4HI GCNGC 1 cut(s) 410
FspBI CTAG 2 cut(s) 839, 981
GluI GCNGC 1 cut(s) 410
GsuI CTGGAG 2 cut(s) 96, 497
HaeIII GGCC 4 cut(s) 102, 703, 930, 987
HapII CCGG 3 cut(s) 201, 700, 1001
HindIII AAGCTT 2 cut(s) 185, 328
HinfI GANTC 6 cut(s) 56, 530, 1036, 1185, 1623, 1641
HpaII CCGG 3 cut(s) 201, 700, 1001
HphI GGTGA 5 cut(s) 163, 190, 983, 1063, 1286
Hpy166II GTNNAC 4 cut(s) 574, 917, 1355, 1597
Hpy188I TCNGA 7 cut(s) 55, 223, 617, 677, 798, 1035, 1129
Hpy188III TCNNGA 8 cut(s) 8, 113, 386, 593, 839, 953, 1259, 1645
Hpy8I GTNNAC 4 cut(s) 574, 917, 1355, 1597
Hpy99I CGWCG 1 cut(s) 870
HpyAV CCTTC 3 cut(s) 941, 962, 1168
HpyCH4III ACNGT 3 cut(s) 269, 569, 1060
HpyCH4IV ACGT 2 cut(s) 709, 1588
HpyCH4V TGCA 5 cut(s) 412, 527, 656, 688, 780
HpyF10VI GCNNNNNNNGC 2 cut(s) 545, 1248
HpyF3I CTNAG 5 cut(s) 723, 795, 1126, 1218, 1475
HpySE526I ACGT 2 cut(s) 709, 1588
Ksp22I TGATCA 1 cut(s) 94
Kzo9I GATC 7 cut(s) 80, 94, 505, 595, 677, 1086, 1602
LmnI GCTCC 5 cut(s) 115, 291, 443, 490, 853
Lsp1109I GCAGC 1 cut(s) 396
LweI GCATC 4 cut(s) 346, 514, 985, 1267
MaeI CTAG 2 cut(s) 839, 981
MaeII ACGT 2 cut(s) 709, 1588
MaeIII GTNAC 4 cut(s) 1102, 1147, 1572, 1584
MalI GATC 7 cut(s) 82, 96, 507, 597, 679, 1088, 1604
MboI GATC 7 cut(s) 80, 94, 505, 595, 677, 1086, 1602
MfeI CAATTG 1 cut(s) 824
MflI RGATCY 2 cut(s) 505, 677
MhlI GDGCHC 1 cut(s) 448
MlyI GAGTC 2 cut(s) 539, 1617
MmeI TCCRAC 1 cut(s) 1460
MseI TTAA 3 cut(s) 1113, 1419, 1547
MslI CAYNNNNRTG 2 cut(s) 306, 453
MspI CCGG 3 cut(s) 201, 700, 1001
MspR9I CCNGG 1 cut(s) 1001
MunI CAATTG 1 cut(s) 824
MwoI GCNNNNNNNGC 2 cut(s) 545, 1248
NciI CCSGG 1 cut(s) 1001
NdeI CATATG 1 cut(s) 1569
NdeII GATC 7 cut(s) 80, 94, 505, 595, 677, 1086, 1602
NlaIV GGNNCC 2 cut(s) 287, 507
NmuCI GTSAC 2 cut(s) 1147, 1584
NspI RCATGY 2 cut(s) 158, 860
PfeI GAWTC 4 cut(s) 56, 1036, 1185, 1641
PkrI GCNGC 1 cut(s) 411
Ple19I CGATCG 1 cut(s) 83
PleI GAGTC 2 cut(s) 538, 1617
PmaCI CACGTG 1 cut(s) 710
PmlI CACGTG 1 cut(s) 710
PpsI GAGTC 2 cut(s) 538, 1617
Ppu21I YACGTR 1 cut(s) 710
Psp124BI GAGCTC 1 cut(s) 448
PspCI CACGTG 1 cut(s) 710
PspN4I GGNNCC 2 cut(s) 287, 507
PspPI GGNCC 3 cut(s) 101, 928, 986
PsrI GAACNNNNNNTAC 2 cut(s) 1557, 1589
PsuI RGATCY 2 cut(s) 505, 677
PvuI CGATCG 1 cut(s) 83
RsaI GTAC 2 cut(s) 18, 1452
RsaNI GTAC 2 cut(s) 17, 1451
RseI CAYNNNNRTG 2 cut(s) 306, 453
SacI GAGCTC 1 cut(s) 448
SaqAI TTAA 3 cut(s) 1113, 1419, 1547
SatI GCNGC 1 cut(s) 410
Sau3AI GATC 7 cut(s) 80, 94, 505, 595, 677, 1086, 1602
Sau96I GGNCC 3 cut(s) 101, 928, 986
ScaI AGTACT 1 cut(s) 18
SchI GAGTC 2 cut(s) 539, 1617
ScrFI CCNGG 1 cut(s) 1001
SduI GDGCHC 1 cut(s) 448
SfaNI GCATC 4 cut(s) 346, 514, 985, 1267
SmiMI CAYNNNNRTG 2 cut(s) 306, 453
SmlI CTYRAG 3 cut(s) 384, 518, 669
SmoI CTYRAG 3 cut(s) 384, 518, 669
SpeI ACTAGT 1 cut(s) 980
SsiI CCGC 3 cut(s) 731, 1162, 1249
SspMI CTAG 2 cut(s) 839, 981
SstI GAGCTC 1 cut(s) 448
StyD4I CCNGG 1 cut(s) 999
StyI CCWWGG 1 cut(s) 178
TaaI ACNGT 3 cut(s) 269, 569, 1060
TaiI ACGT 2 cut(s) 712, 1591
TaqI TCGA 3 cut(s) 9, 606, 1462
TatI WGTACW 2 cut(s) 16, 1450
TfiI GAWTC 4 cut(s) 56, 1036, 1185, 1641
Tru1I TTAA 3 cut(s) 1113, 1419, 1547
Tru9I TTAA 3 cut(s) 1113, 1419, 1547
TscAI CASTG 1 cut(s) 572
TseFI GTSAC 2 cut(s) 1147, 1584
TseI GCWGC 1 cut(s) 409
Tsp45I GTSAC 2 cut(s) 1147, 1584
TspDTI ATGAA 9 cut(s) 27, 261, 339, 443, 488, 788, 923, 1067, 1497
TspRI CASTG 1 cut(s) 572
XapI RAATTY 6 cut(s) 249, 348, 624, 718, 1005, 1264
XbaI TCTAGA 1 cut(s) 838
XceI RCATGY 2 cut(s) 158, 860
XcmI CCANNNNNNNNNTGG 3 cut(s) 185, 711, 1337
XmiI GTMKAC 1 cut(s) 573
XspI CTAG 2 cut(s) 839, 981
ZrmI AGTACT 1 cut(s) 18
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.