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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 331 |
| AciI | CCGC | 2 cut(s) | 215, 400 |
| AclWI | GGATC | 2 cut(s) | 304, 364 |
| AcsI | RAATTY | 1 cut(s) | 413 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 152 |
| AgsI | TTSAA | 1 cut(s) | 143 |
| AjnI | CCWGG | 3 cut(s) | 53, 306, 380 |
| Alw21I | GWGCWC | 1 cut(s) | 389 |
| Alw26I | GTCTC | 1 cut(s) | 184 |
| AlwI | GGATC | 2 cut(s) | 304, 364 |
| ApoI | RAATTY | 1 cut(s) | 413 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 147, 231 |
| AspLEI | GCGC | 1 cut(s) | 399 |
| AsuC2I | CCSGG | 1 cut(s) | 153 |
| AsuHPI | GGTGA | 1 cut(s) | 296 |
| AsuII | TTCGAA | 2 cut(s) | 220, 411 |
| AxyI | CCTNAGG | 1 cut(s) | 237 |
| BanII | GRGCYC | 1 cut(s) | 285 |
| Bbv12I | GWGCWC | 1 cut(s) | 389 |
| BccI | CCATC | 4 cut(s) | 21, 199, 294, 358 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 231, 265 |
| BciT130I | CCWGG | 3 cut(s) | 55, 308, 382 |
| BclI | TGATCA | 1 cut(s) | 75 |
| BcnI | CCSGG | 1 cut(s) | 153 |
| BcoDI | GTCTC | 1 cut(s) | 184 |
| BfaI | CTAG | 1 cut(s) | 135 |
| BfuAI | ACCTGC | 1 cut(s) | 331 |
| BisI | GCNGC | 1 cut(s) | 400 |
| BlsI | GCNGC | 1 cut(s) | 401 |
| Bme1390I | CCNGG | 4 cut(s) | 55, 153, 308, 382 |
| BmrFI | CCNGG | 4 cut(s) | 55, 153, 308, 382 |
| BmsI | GCATC | 2 cut(s) | 60, 251 |
| Bpu14I | TTCGAA | 2 cut(s) | 220, 411 |
| BpuMI | CCSGG | 1 cut(s) | 153 |
| Bsa29I | ATCGAT | 1 cut(s) | 85 |
| BsaAI | YACGTR | 1 cut(s) | 368 |
| BsaBI | GATNNNNATC | 1 cut(s) | 74 |
| BsaJI | CCNNGG | 2 cut(s) | 54, 307 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 152 |
| Bse21I | CCTNAGG | 1 cut(s) | 237 |
| Bse8I | GATNNNNATC | 1 cut(s) | 74 |
| BseBI | CCWGG | 3 cut(s) | 55, 308, 382 |
| BseCI | ATCGAT | 1 cut(s) | 85 |
| BseDI | CCNNGG | 2 cut(s) | 54, 307 |
| BseGI | GGATG | 1 cut(s) | 389 |
| BseJI | GATNNNNATC | 1 cut(s) | 74 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 152 |
| BseMII | CTCAG | 2 cut(s) | 228, 324 |
| Bsh1285I | CGRYCG | 1 cut(s) | 323 |
| BshVI | ATCGAT | 1 cut(s) | 85 |
| BsiEI | CGRYCG | 1 cut(s) | 323 |
| BsiHKAI | GWGCWC | 1 cut(s) | 389 |
| BsiSI | CCGG | 1 cut(s) | 153 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 152 |
| BsmAI | GTCTC | 1 cut(s) | 184 |
| Bsp119I | TTCGAA | 2 cut(s) | 220, 411 |
| Bsp1286I | GDGCHC | 2 cut(s) | 285, 389 |
| Bsp143I | GATC | 5 cut(s) | 75, 86, 260, 296, 356 |
| BspACI | CCGC | 2 cut(s) | 215, 400 |
| BspCNI | CTCAG | 2 cut(s) | 229, 325 |
| BspDI | ATCGAT | 1 cut(s) | 85 |
| BspMI | ACCTGC | 1 cut(s) | 331 |
| BspPI | GGATC | 2 cut(s) | 304, 364 |
| BspT104I | TTCGAA | 2 cut(s) | 220, 411 |
| BssECI | CCNNGG | 2 cut(s) | 54, 307 |
| BssMI | GATC | 5 cut(s) | 75, 86, 260, 296, 356 |
| Bst2UI | CCWGG | 3 cut(s) | 55, 308, 382 |
| Bst4CI | ACNGT | 1 cut(s) | 8 |
| Bst6I | CTCTTC | 1 cut(s) | 121 |
| BstBAI | YACGTR | 1 cut(s) | 368 |
| BstBI | TTCGAA | 2 cut(s) | 220, 411 |
| BstC8I | GCNNGC | 1 cut(s) | 31 |
| BstDEI | CTNAG | 2 cut(s) | 237, 333 |
| BstF5I | GGATG | 1 cut(s) | 389 |
| BstHHI | GCGC | 1 cut(s) | 399 |
| BstKTI | GATC | 5 cut(s) | 78, 89, 263, 299, 359 |
| BstMAI | GTCTC | 1 cut(s) | 184 |
| BstMBI | GATC | 5 cut(s) | 75, 86, 260, 296, 356 |
| BstMCI | CGRYCG | 1 cut(s) | 323 |
| BstNI | CCWGG | 3 cut(s) | 55, 308, 382 |
| BstSCI | CCNGG | 4 cut(s) | 53, 151, 306, 380 |
| BstSNI | TACGTA | 1 cut(s) | 368 |
| BstX2I | RGATCY | 1 cut(s) | 296 |
| BstYI | RGATCY | 1 cut(s) | 296 |
| Bsu15I | ATCGAT | 1 cut(s) | 85 |
| Bsu36I | CCTNAGG | 1 cut(s) | 237 |
| BsuTUI | ATCGAT | 1 cut(s) | 85 |
| BtsCI | GGATG | 1 cut(s) | 389 |
| BveI | ACCTGC | 1 cut(s) | 331 |
| Cac8I | GCNNGC | 1 cut(s) | 31 |
| CfoI | GCGC | 1 cut(s) | 399 |
| ClaI | ATCGAT | 1 cut(s) | 85 |
| CviAII | CATG | 1 cut(s) | 176 |
| CviJI | RGCY | 4 cut(s) | 53, 134, 211, 283 |
| CviKI_1 | RGCY | 4 cut(s) | 53, 134, 211, 283 |
| DdeI | CTNAG | 2 cut(s) | 237, 333 |
| DpnI | GATC | 5 cut(s) | 77, 88, 262, 298, 358 |
| DpnII | GATC | 5 cut(s) | 75, 86, 260, 296, 356 |
| Eam1104I | CTCTTC | 1 cut(s) | 121 |
| EarI | CTCTTC | 1 cut(s) | 121 |
| Eco105I | TACGTA | 1 cut(s) | 368 |
| Eco24I | GRGCYC | 1 cut(s) | 285 |
| Eco81I | CCTNAGG | 1 cut(s) | 237 |
| EcoRI | GAATTC | 1 cut(s) | 413 |
| EcoRII | CCWGG | 3 cut(s) | 53, 306, 380 |
| EcoT38I | GRGCYC | 1 cut(s) | 285 |
| FaeI | CATG | 1 cut(s) | 179 |
| FaiI | YATR | 3 cut(s) | 177, 278, 365 |
| FatI | CATG | 1 cut(s) | 175 |
| FbaI | TGATCA | 1 cut(s) | 75 |
| Fnu4HI | GCNGC | 1 cut(s) | 400 |
| FokI | GGATG | 1 cut(s) | 376 |
| FriOI | GRGCYC | 1 cut(s) | 285 |
| Fsp4HI | GCNGC | 1 cut(s) | 400 |
| FspBI | CTAG | 1 cut(s) | 135 |
| GlaI | GCGC | 1 cut(s) | 398 |
| GluI | GCNGC | 1 cut(s) | 400 |
| HapII | CCGG | 1 cut(s) | 153 |
| HhaI | GCGC | 1 cut(s) | 399 |
| Hin1II | CATG | 1 cut(s) | 179 |
| Hin6I | GCGC | 1 cut(s) | 397 |
| HinP1I | GCGC | 1 cut(s) | 397 |
| HinfI | GANTC | 1 cut(s) | 39 |
| HpaII | CCGG | 1 cut(s) | 153 |
| HphI | GGTGA | 1 cut(s) | 296 |
| Hpy166II | GTNNAC | 1 cut(s) | 167 |
| Hpy188I | TCNGA | 3 cut(s) | 334, 361, 419 |
| Hpy188III | TCNNGA | 3 cut(s) | 236, 294, 354 |
| Hpy8I | GTNNAC | 1 cut(s) | 167 |
| HpyCH4III | ACNGT | 1 cut(s) | 8 |
| HpyCH4IV | ACGT | 1 cut(s) | 367 |
| HpyF3I | CTNAG | 2 cut(s) | 237, 333 |
| HpySE526I | ACGT | 1 cut(s) | 367 |
| Hsp92II | CATG | 1 cut(s) | 179 |
| HspAI | GCGC | 1 cut(s) | 397 |
| Ksp22I | TGATCA | 1 cut(s) | 75 |
| Kzo9I | GATC | 5 cut(s) | 75, 86, 260, 296, 356 |
| LmnI | GCTCC | 1 cut(s) | 384 |
| LweI | GCATC | 2 cut(s) | 60, 251 |
| MaeI | CTAG | 1 cut(s) | 135 |
| MaeII | ACGT | 1 cut(s) | 367 |
| MaeIII | GTNAC | 1 cut(s) | 255 |
| MalI | GATC | 5 cut(s) | 77, 88, 262, 298, 358 |
| MboI | GATC | 5 cut(s) | 75, 86, 260, 296, 356 |
| MboII | GAAGA | 5 cut(s) | 8, 108, 111, 239, 291 |
| MflI | RGATCY | 1 cut(s) | 296 |
| MhlI | GDGCHC | 2 cut(s) | 285, 389 |
| MluCI | AATT | 4 cut(s) | 104, 127, 147, 413 |
| MnlI | CCTC | 4 cut(s) | 105, 232, 279, 328 |
| MroXI | GAANNNNTTC | 2 cut(s) | 147, 231 |
| MseI | TTAA | 2 cut(s) | 63, 315 |
| MspI | CCGG | 1 cut(s) | 153 |
| MspR9I | CCNGG | 4 cut(s) | 55, 153, 308, 382 |
| MvaI | CCWGG | 3 cut(s) | 55, 308, 382 |
| NciI | CCSGG | 1 cut(s) | 153 |
| NdeII | GATC | 5 cut(s) | 75, 86, 260, 296, 356 |
| NlaIII | CATG | 1 cut(s) | 179 |
| NspV | TTCGAA | 2 cut(s) | 220, 411 |
| PdmI | GAANNNNTTC | 2 cut(s) | 147, 231 |
| PfeI | GAWTC | 1 cut(s) | 39 |
| PkrI | GCNGC | 1 cut(s) | 401 |
| Ppu21I | YACGTR | 1 cut(s) | 368 |
| Psp6I | CCWGG | 3 cut(s) | 53, 306, 380 |
| PspGI | CCWGG | 3 cut(s) | 53, 306, 380 |
| PsuI | RGATCY | 1 cut(s) | 296 |
| SaqAI | TTAA | 2 cut(s) | 63, 315 |
| SatI | GCNGC | 1 cut(s) | 400 |
| Sau3AI | GATC | 5 cut(s) | 75, 86, 260, 296, 356 |
| ScrFI | CCNGG | 4 cut(s) | 55, 153, 308, 382 |
| SduI | GDGCHC | 2 cut(s) | 285, 389 |
| SetI | ASST | 3 cut(s) | 271, 345, 370 |
| SfaNI | GCATC | 2 cut(s) | 60, 251 |
| SfuI | TTCGAA | 2 cut(s) | 220, 411 |
| SnaBI | TACGTA | 1 cut(s) | 368 |
| Sse9I | AATT | 4 cut(s) | 104, 127, 147, 413 |
| SsiI | CCGC | 2 cut(s) | 215, 400 |
| SspMI | CTAG | 1 cut(s) | 135 |
| StyD4I | CCNGG | 4 cut(s) | 53, 151, 306, 380 |
| TaaI | ACNGT | 1 cut(s) | 8 |
| TaiI | ACGT | 1 cut(s) | 370 |
| TaqI | TCGA | 4 cut(s) | 68, 85, 220, 411 |
| TaqII | GACCGA | 1 cut(s) | 309 |
| TasI | AATT | 4 cut(s) | 104, 127, 147, 413 |
| TauI | GCSGC | 1 cut(s) | 402 |
| TfiI | GAWTC | 1 cut(s) | 39 |
| Tru1I | TTAA | 2 cut(s) | 63, 315 |
| Tru9I | TTAA | 2 cut(s) | 63, 315 |
| TspDTI | ATGAA | 3 cut(s) | 17, 188, 265 |
| XapI | RAATTY | 1 cut(s) | 413 |
| XmnI | GAANNNNTTC | 2 cut(s) | 147, 231 |
| XspI | CTAG | 1 cut(s) | 135 |