pycom2874g00060
RLK Family

Cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00002874
OG0000146
Physical Location & Seq
Reverse (-)
56165 .. 56521
357 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom2874g00060.1

Sequence Viewer

Length: 357 bp
ATGATCTTTATCTTGGCATCAGAGCCGGGTTTTATGGCCTGGTATTTTTTCTCCAACCTTAAATTCAAACTCAACGATTCGAACTTTAATATTTGGTCGAAGATGATGGAAGTTCAAACGGAAGGATTAAATAAGATGGGTTATCTCAATGGGAAGACTCCGGCTGTGGATGAGACAGAACCCGGGTTTTTAAAGTGGCACACTGAGGATGCAATCGTCCGAGGATGGCTGCTAAAAACTATGGAACCACATCTGTTAGGGCTATTCATTGAGTTACTAACGGCACATGATATTTGGGAGAGCGTTAGTCAGATGTTCTATGATGTGACAGACGAGTCCCAGTACTATGGGCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Retrotran_gag_2

Protein Analysis

119

Amino Acids

13.8

Weight (kDa)

4.65

Isoelectric Point (pI)

45.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000146)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19561 FvH4_1g25371 FvH4_1g25961 FvH4_2g00352 FvH4_2g00861 FvH4_2g04952 FvH4_2g05413 FvH4_2g07531 FvH4_2g08961 FvH4_2g25141 FvH4_2g27292 FvH4_3g18164 FvH4_3g19921 FvH4_3g43870 FvH4_4g14441 FvH4_4g15581 FvH4_4g15582 FvH4_4g16890 FvH4_4g19782 FvH4_4g33444 FvH4_4g33921 FvH4_5g22281 FvH4_5g22282 FvH4_5g32571 FvH4_5g37451 FvH4_5g39631 FvH4_6g04721 FvH4_6g11661 FvH4_6g20571 FvH4_6g32791 FvH4_7g23184
prunus_persica Prupe.1G090900_v2.0.a1 Prupe.1G241200_v2.0.a1 Prupe.1G534100_v2.0.a1 Prupe.3G076700_v2.0.a1 Prupe.4G150800_v2.0.a1 Prupe.4G181800_v2.0.a1 Prupe.4G240900_v2.0.a1 Prupe.4G247600_v2.0.a1 Prupe.6G115900_v2.0.a1 Prupe.6G327500_v2.0.a1 Prupe.8G071700_v2.0.a1
pyrus_communis pycom06g11480 pycom10g08330 pycom15g04320 pycom15g04350 pycom15g12620 pycom2874g00060
rosa_chinensis RchiOBHm_Chr1g0317241 RchiOBHm_Chr1g0341801 RchiOBHm_Chr1g0341931 RchiOBHm_Chr1g0359621 RchiOBHm_Chr2g0113741 RchiOBHm_Chr2g0117261 RchiOBHm_Chr2g0138831 RchiOBHm_Chr2g0141421 RchiOBHm_Chr2g0145281 RchiOBHm_Chr2g0152991 RchiOBHm_Chr3g0448001 RchiOBHm_Chr3g0450981 RchiOBHm_Chr3g0455161 RchiOBHm_Chr3g0457401 RchiOBHm_Chr3g0460001 RchiOBHm_Chr4g0387431 RchiOBHm_Chr4g0388741 RchiOBHm_Chr4g0444201 RchiOBHm_Chr4g0444261 RchiOBHm_Chr5g0010221 RchiOBHm_Chr6g0271721 RchiOBHm_Chr6g0274641 RchiOBHm_Chr6g0294691 RchiOBHm_Chr7g0237031 RchiOBHm_Chr7g0238461 RchiOBHm_Chr7g0239981
rosa_laevigata RLG00000009017
rosa_multiflora Rmu_co7986768.1_g000001 Rmu_co8302239.1_g000001 Rmu_co8485799.1_g000001 Rmu_sc0000061.1_g000013 Rmu_sc0000303.1_g000034 Rmu_sc0000303.1_g000035 Rmu_sc0000315.1_g000017 Rmu_sc0000349.1_g000003 Rmu_sc0000498.1_g000068 Rmu_sc0000508.1_g000014 Rmu_sc0000602.1_g000001 Rmu_sc0000633.1_g000002 Rmu_sc0000638.1_g000030 Rmu_sc0000756.1_g000016 Rmu_sc0000756.1_g000017 Rmu_sc0000785.1_g000037 Rmu_sc0000795.1_g000017 Rmu_sc0000796.1_g000030 Rmu_sc0000863.1_g000023 Rmu_sc0000920.1_g000021 Rmu_sc0000960.1_g000015 Rmu_sc0000982.1_g000102 Rmu_sc0001036.1_g000008 Rmu_sc0001036.1_g000009 Rmu_sc0001643.1_g000029 Rmu_sc0001687.1_g000026 Rmu_sc0001708.1_g000037 Rmu_sc0001768.1_g000015 Rmu_sc0001790.1_g000022 Rmu_sc0002180.1_g000007 Rmu_sc0002211.1_g000006 Rmu_sc0002270.1_g000010 Rmu_sc0002404.1_g000031 Rmu_sc0002858.1_g000016 Rmu_sc0003063.1_g000028 Rmu_sc0003730.1_g000017 Rmu_sc0004087.1_g000020 Rmu_sc0004618.1_g000048 Rmu_sc0004724.1_g000005 Rmu_sc0005167.1_g000036 Rmu_sc0005781.1_g000003 Rmu_sc0005878.1_g000012 Rmu_sc0006011.1_g000003 Rmu_sc0006343.1_g000011 Rmu_sc0006573.1_g000007 Rmu_sc0006599.1_g000007 Rmu_sc0006803.1_g000014 Rmu_sc0006836.1_g000002 Rmu_sc0006849.1_g000002 Rmu_sc0007109.1_g000009 Rmu_sc0009268.1_g000016 Rmu_sc0009618.1_g000017 Rmu_sc0009674.1_g000009 Rmu_sc0010244.1_g000009 Rmu_sc0011151.1_g000004 Rmu_sc0011356.1_g000001 Rmu_sc0011674.1_g000002 Rmu_sc0011778.1_g000016 Rmu_sc0012342.1_g000001 Rmu_sc0012467.1_g000002 Rmu_sc0012574.1_g000003 Rmu_sc0012913.1_g000005 Rmu_sc0013612.1_g000020 Rmu_sc0014824.1_g000001 Rmu_sc0017869.1_g000002 Rmu_sc0018085.1_g000003 Rmu_sc0024789.1_g000001 Rmu_sc0025106.1_g000013 Rmu_sc0034502.1_g000001 Rmu_sc0034547.1_g000001 Rmu_sc0035094.1_g000001 Rmu_sc0035095.1_g000001 Rmu_ssc0000100.1_g000027 Rmu_ssc0000244.1_g000005 Rmu_ssc0000371.1_g000029 Rmu_ssc0000381.1_g000004
rosa_roxburghii Rroxscaffold_1G00015480 Rroxscaffold_1G00023580 Rroxscaffold_1G00039340 Rroxscaffold_1G00047080 Rroxscaffold_2G00095820 Rroxscaffold_2G00125200 Rroxscaffold_2G00128330 Rroxscaffold_3G00270860
rosa_rugosa Rorug06G0120700
rosa_samantha Rh3BG173100 Rh6AG089600 Rh7AG488100 Rh7DG389600
rosa_wichuraiana Rw0G001150 Rw0G001990 Rw0G002010 Rw0G019540 Rw1G013730 Rw1G024110 Rw1G029190 Rw2G018850 Rw2G021090 Rw2G022260 Rw2G023180 Rw2G027280 Rw3G010190 Rw3G020660 Rw3G029380 Rw4G002390 Rw4G002690 Rw4G033260 Rw5G011960 Rw5G023110 Rw5G025250 Rw5G028950 Rw5G033740 Rw5G033950 Rw5G037900 Rw6G009640 Rw6G011620 Rw6G027720 Rw7G010680 Rw7G042070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 334
AcsI RAATTY 1 cut(s) 62
AfaI GTAC 1 cut(s) 344
AgsI TTSAA 2 cut(s) 67, 116
AjnI CCWGG 1 cut(s) 38
AjuI GAANNNNNNNTTGG 2 cut(s) 47, 79
Alw26I GTCTC 1 cut(s) 167
Ama87I CYCGRG 1 cut(s) 182
AoxI GGCC 1 cut(s) 36
ApeKI GCWGC 1 cut(s) 229
ApoI RAATTY 1 cut(s) 62
AsuC2I CCSGG 3 cut(s) 27, 183, 184
AsuII TTCGAA 1 cut(s) 80
AvaI CYCGRG 1 cut(s) 182
BbsI GAAGAC 1 cut(s) 161
BbvI GCAGC 1 cut(s) 216
BccI CCATC 3 cut(s) 100, 130, 219
BceAI ACGGC 1 cut(s) 297
BciT130I CCWGG 1 cut(s) 40
BcnI CCSGG 3 cut(s) 27, 183, 184
BcoDI GTCTC 1 cut(s) 167
BisI GCNGC 1 cut(s) 230
BlsI GCNGC 1 cut(s) 231
BmcAI AGTACT 1 cut(s) 344
Bme1390I CCNGG 4 cut(s) 27, 40, 183, 184
BmeT110I CYCGRG 1 cut(s) 182
BmiI GGNNCC 1 cut(s) 246
BmrFI CCNGG 4 cut(s) 27, 40, 183, 184
BmrI ACTGGG 1 cut(s) 334
BmsI GCATC 2 cut(s) 26, 199
BmuI ACTGGG 1 cut(s) 334
BpiI GAAGAC 1 cut(s) 161
Bpu14I TTCGAA 1 cut(s) 80
BpuMI CCSGG 3 cut(s) 27, 183, 184
BsaBI GATNNNNATC 1 cut(s) 8
BsaJI CCNNGG 2 cut(s) 182, 220
Bse1I ACTGG 1 cut(s) 340
Bse8I GATNNNNATC 1 cut(s) 8
BseBI CCWGG 1 cut(s) 40
BseDI CCNNGG 2 cut(s) 182, 220
BseGI GGATG 3 cut(s) 175, 214, 230
BseJI GATNNNNATC 1 cut(s) 8
BseMII CTCAG 1 cut(s) 195
BseNI ACTGG 1 cut(s) 340
BseXI GCAGC 1 cut(s) 216
BshFI GGCC 1 cut(s) 38
BsiHKCI CYCGRG 1 cut(s) 182
BsiSI CCGG 3 cut(s) 26, 161, 183
BslFI GGGAC 1 cut(s) 322
BsmAI GTCTC 1 cut(s) 167
BsmFI GGGAC 1 cut(s) 322
BsnI GGCC 1 cut(s) 38
BsoBI CYCGRG 1 cut(s) 182
Bsp119I TTCGAA 1 cut(s) 80
Bsp143I GATC 1 cut(s) 3
BspANI GGCC 1 cut(s) 38
BspCNI CTCAG 1 cut(s) 196
BspLI GGNNCC 1 cut(s) 246
BspT104I TTCGAA 1 cut(s) 80
BsrI ACTGG 1 cut(s) 340
BssECI CCNNGG 2 cut(s) 182, 220
BssMI GATC 1 cut(s) 3
Bst2UI CCWGG 1 cut(s) 40
BstBI TTCGAA 1 cut(s) 80
BstDEI CTNAG 1 cut(s) 204
BstF5I GGATG 3 cut(s) 175, 214, 230
BstKTI GATC 1 cut(s) 6
BstMAI GTCTC 1 cut(s) 167
BstMBI GATC 1 cut(s) 3
BstNI CCWGG 1 cut(s) 40
BstSCI CCNGG 4 cut(s) 25, 38, 181, 182
BstV1I GCAGC 1 cut(s) 216
BstV2I GAAGAC 1 cut(s) 161
BstXI CCANNNNNNTGG 1 cut(s) 347
BsuRI GGCC 1 cut(s) 38
BtsCI GGATG 3 cut(s) 175, 214, 230
BtsIMutI CAGTG 1 cut(s) 201
Cfr9I CCCGGG 1 cut(s) 182
Csp6I GTAC 1 cut(s) 343
CviAII CATG 1 cut(s) 287
CviJI RGCY 6 cut(s) 25, 38, 164, 229, 262, 352
CviKI_1 RGCY 6 cut(s) 25, 38, 164, 229, 262, 352
CviQI GTAC 1 cut(s) 343
DdeI CTNAG 1 cut(s) 204
DpnI GATC 1 cut(s) 5
DpnII GATC 1 cut(s) 3
DraI TTTAAA 1 cut(s) 192
DrdI GACNNNNNNGTC 1 cut(s) 334
DseDI GACNNNNNNGTC 1 cut(s) 334
Eco88I CYCGRG 1 cut(s) 182
EcoRII CCWGG 1 cut(s) 38
FaeI CATG 1 cut(s) 290
FaiI YATR 6 cut(s) 35, 242, 288, 321, 348, 355
FaqI GGGAC 1 cut(s) 322
FatI CATG 1 cut(s) 286
Fnu4HI GCNGC 1 cut(s) 230
FokI GGATG 3 cut(s) 182, 221, 237
Fsp4HI GCNGC 1 cut(s) 230
GluI GCNGC 1 cut(s) 230
HaeIII GGCC 1 cut(s) 38
HapII CCGG 3 cut(s) 26, 161, 183
Hin1II CATG 1 cut(s) 290
HinfI GANTC 3 cut(s) 77, 157, 335
HpaII CCGG 3 cut(s) 26, 161, 183
Hpy188I TCNGA 3 cut(s) 22, 221, 312
HpyAV CCTTC 1 cut(s) 116
HpyCH4V TGCA 1 cut(s) 212
HpyF3I CTNAG 1 cut(s) 204
Hsp92II CATG 1 cut(s) 290
Kzo9I GATC 1 cut(s) 3
LpnPI CCDG 6 cut(s) 25, 39, 52, 174, 196, 353
Lsp1109I GCAGC 1 cut(s) 216
LweI GCATC 2 cut(s) 26, 199
MaeIII GTNAC 2 cut(s) 273, 325
MalI GATC 1 cut(s) 5
MboI GATC 1 cut(s) 3
MboII GAAGA 2 cut(s) 112, 166
MluCI AATT 1 cut(s) 62
MlyI GAGTC 2 cut(s) 151, 344
MmeI TCCRAC 1 cut(s) 78
MnlI CCTC 2 cut(s) 199, 215
MseI TTAA 4 cut(s) 60, 87, 128, 191
MspI CCGG 3 cut(s) 26, 161, 183
MspR9I CCNGG 4 cut(s) 27, 40, 183, 184
MvaI CCWGG 1 cut(s) 40
NciI CCSGG 3 cut(s) 27, 183, 184
NdeII GATC 1 cut(s) 3
NlaIII CATG 1 cut(s) 290
NlaIV GGNNCC 1 cut(s) 246
NmuCI GTSAC 1 cut(s) 325
NspV TTCGAA 1 cut(s) 80
PfeI GAWTC 1 cut(s) 77
PkrI GCNGC 1 cut(s) 231
PleI GAGTC 2 cut(s) 151, 343
PpsI GAGTC 2 cut(s) 151, 343
Psp6I CCWGG 1 cut(s) 38
PspGI CCWGG 1 cut(s) 38
PspN4I GGNNCC 1 cut(s) 246
RsaI GTAC 1 cut(s) 344
RsaNI GTAC 1 cut(s) 343
SaqAI TTAA 4 cut(s) 60, 87, 128, 191
SatI GCNGC 1 cut(s) 230
Sau3AI GATC 1 cut(s) 3
ScaI AGTACT 1 cut(s) 344
SchI GAGTC 2 cut(s) 151, 344
ScrFI CCNGG 4 cut(s) 27, 40, 183, 184
SetI ASST 1 cut(s) 60
SfaNI GCATC 2 cut(s) 26, 199
SfuI TTCGAA 1 cut(s) 80
SmaI CCCGGG 1 cut(s) 184
Sse9I AATT 1 cut(s) 62
SspI AATATT 1 cut(s) 91
StyD4I CCNGG 4 cut(s) 25, 38, 181, 182
TaqI TCGA 2 cut(s) 80, 98
TasI AATT 1 cut(s) 62
TatI WGTACW 1 cut(s) 342
TfiI GAWTC 1 cut(s) 77
Tru1I TTAA 4 cut(s) 60, 87, 128, 191
Tru9I TTAA 4 cut(s) 60, 87, 128, 191
TscAI CASTG 1 cut(s) 208
TseFI GTSAC 1 cut(s) 325
TseI GCWGC 1 cut(s) 229
Tsp45I GTSAC 1 cut(s) 325
TspDTI ATGAA 1 cut(s) 256
TspGWI ACGGA 1 cut(s) 134
TspMI CCCGGG 1 cut(s) 182
TspRI CASTG 1 cut(s) 208
XapI RAATTY 1 cut(s) 62
XmaI CCCGGG 1 cut(s) 182
ZrmI AGTACT 1 cut(s) 344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.