pycom15g04320
RLK Family

cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0000146
Physical Location & Seq
Forward (+)
2588802 .. 2592992
4191 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g04320.1

Sequence Viewer

Length: 3336 bp
ATGTTGCAGCTCTATCCAAAAGGTTCGCCTCTTGCTCATGACGTTTCGAGGGCAATCTTAAATGTGACCGAGGGAGAACAAATGAAAGGTATTCAAGAAACAAGCGAGTTCCTTGATAGCTTTTGGGGCCTCTTTCTCATTGCTGTGTGTGTTTCACTAGTTGCTCTGCTCATATTTGCTGGCATCTTCTTGTGTAGAGAACTCGAGAATATATCGAGGGAACTAAAAGATCTTGATCGAGGAACGACCAATTGGACACCAAATCATTTGCTTTTCGGTGTAATGCACTTACACCCCCCTATGAACCGCCCGCTGACCCACCAACTCCACTGCCCGCGGAACCACCCGCTCCACTGCCCGCTCAACCACCCGCTCCACTGCCCGCTCAACCACCCGCTCAACCTCCTGCTCTGCCTTGAAAGCTTAAATTCAATTTCACATGTTGCCACTTTAATCTCTCTCACCCTGTTGGTGCAATCGTCTAAGTTCAATGGTGGTCTACTGCTTAGAGCCAAGTTTGGTTACCTGATTCCTAGATTCTTATCCATCCATTTAAGATTGAATCAAATCTGTTGGAGTTTTGACAAGGAAGAGAAGAAGAAAAATGAATGTCAAAACTCAGAGGTTGGCTCCAATGAAGTCCTAACTTGTTTCTATTCATTTTATGATTTGTTTGGTGTAGACCTTTCTCACGCCACTTGGGAGGAGACTTGTTCTTCCTCTTTCGTATGGCTTCTGCACATGCATTTATACATTGCTCTCTCTACCTCATTATATAGAGAGGTGTGTTGGTGTTCACTCTCTTGTACATGTTTTACTCACTTGGTGCTCCTCAAGAATAGGTCCAATGTTGGCTATCAAGTAGGTTCTTTTATTTACGGGATTATGAAACAAATAGGATTTCAGGATGAAGATCTCAAGATTCATAAATTCGCAGAAGAGTTGAATCAACTTTTTCAAAATGGTCAAAATGGTAGTAGAAAATACGGTACTTCCGCAGCTTTTGATGAAACACCCTACATGAACTTTTTCTTGCAACATAATATACCTCAATGGACCAAACTTTTTTCTTTTTGTTTATTGATAAACGATAACAGGGATCGAATTGCATCAGGGTACATAGGCATCATTACAAATCATACTGTAAATTTGTTATGTGGTTGCAAAGAAATCGGGAAGGATTACAAATTAAATAAACCAGCATCCTTTAGTCTATTGTCAATCAGAATGCTCTCATTTAAAGACTTTTACAAAGATATTTCAATTTCTATAAAATCGATAGGATACGATATGATAGAAAATAAAGACAATTTACTATTATGGGTAGGCTTTATACGTGCGGATAAAAACTATAGGACTCGCACATCTATTCTCATCAGATTGTTTTTCATTATTAATTTGTCTACTTGGCCTATACTTTGTAGGGTTTCTTATTCTATTTCTTCTAGTTTTAACATATCGGCCTTCTTATCAATATTAGGAAAAATAGGGAATGTAAATAATAGGAGTCCTTTATTAAGTAGGTTTGTGTATATGTTATTTGTTTCCTTTAATAGAAGAAAATTAATTCCCGTAAAAGTTCTATTTGGTATCAAGAGCCAAAACCCTAGTTTTCTTTTCCGCTGTGCTTGGTCGACCTTATTTCTGCAAATCTTCTGCAGAAAACAGGGTTGCCGTGTGGGTGTGGTACTGTTGTGTGTGCAGTCTGCTGCTGTGTATTGCTGTGCAGTGATGTTCTGTTGCTGTATATTGCTGTGCAGCACAATACCTTGCTGCTGCCGTGAGGGTTGTGTACAAATTGCTGTGTTTTTCTGCAATTGTACCGTGTCTGTTGCTACTGCCGTGAGGGTGTGTACTTGTCTGCTGCCTGTCTGTTACATTAGAAATTGCTGTGTTTTTTTTTTTCTGCAATTGTACCGTGTCTGTTTAATGGACTCAAGAGGCAGTGGCGTGTTGAAGTTAGAGGGCAGTAATTCAACCAATAATCAAGTAGTGAATCCTGTCGTTATTCAGAGTGATGCGTCTGCTGTACCAAATACCGTGAAATTAAATGGATCGAATTATCCTCTTTGGTCCAAGGTTTTGGAGATGCACATTGCTGGACGCGGTAGGAAGGGCTTTGTGACTGGGAATATCAAGGAGCCTGCTGAGGCTAGTGCTGAGTATGAGGCGTGGGAGACAGGGAATGCAATAGTAAAAGGGTGGTTGATCAATTCCATGCAACCGGCTATCATGGGATTTTTTATTCACCTACGTACTGCTAAAGAAGTTTGGGAAGAGGTGGCTCGAACTTATTATGATGGTTCAGATATTTCTCAGATTTATGAATTGAAGGTTAAATCGTTCAGACTCCGTCAAGATGGTCGGCCAGTTGGTGTGTATTATGCTGATCTCAAGTCCGTTTGGCAGGAGTTAGATCAACGGAGACCAATCAAGATGGTATATGCTGTGGATTTCAAGACACTTCGAGAAGAAATTCAGGTTGATCGTGTGTATGCTTTTTTGGCGGGTTTGGATGATGTGTTTGATAAAGTACGTAGTGATATTTTACGTACTCAGCCCTTACCTTCTGTTGAGGAGGTGTTTTCTGTTGTTCGACGTGAAGCTCAACGACATGCTACCATGATGGGTGGGAGTAACAATCAAGGAGGATTGCCGTCCATGGCAATGATCTCTCGGCCAGCTGGTACTCATCATGCTAGTAATCTTTCTAATCAATCTTTAAATTCTCGACCTTTCAACCGAGAAAATAAAGATGATTTGAAGTGTACTTTTTGTGGACAAACCCGTCATACTGAGGATACATGCTTTACTAAGCATGGGGTGCCTGATTGGTTTCCAGAGTTAAAGAAAAAATTGCGTGCCAAGGAGCGTGGAGCAGCCGGATCCAGTGGAGGCCGTGCCTCCCTTGCTGCGGCTACACCCACAGTCCAGGAAGCCGTGCCTAGTTCTAGTAACTCCAGTCAGAATTTGTTGCTTGGTACTCGGGGTGACTCGTCTTTTGACACAGGTACCGTGGGACGTGTTCTCTTAGCCTCCGAAAATCAGCACCATACAGGTTGGATTCTGGACTCTGGGGCAACGGATCATATGACATATGATAAAAATATGTTTCAATACATGACCACATCTCACAGGAAAAATATATCAACTGCCAATGACTTACATTGTGAAGTATGTATTCTTGCTAAAAGCCATCGGGCTTCGTTTCCTCCTAGTATGAATAAAAGAGCTTTACCGTACTTGTGCACTCTGATGTTTGGGGACCATCTCCTGTTGTTACTTCTTCTGGGATTAAGTGGTTTGTTACGTTTGTTGATGATTGCACTCGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 Retrotran_gag_3 Retrotrans_gag gag_pre-integrs rve

Protein Analysis

1112

Amino Acids

125.37

Weight (kDa)

8.83

Isoelectric Point (pI)

38.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pol_BBD PF22936 1021 - 1059 1.3e-07 Pol polyprotein, beta-barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000146)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19561 FvH4_1g25371 FvH4_1g25961 FvH4_2g00352 FvH4_2g00861 FvH4_2g04952 FvH4_2g05413 FvH4_2g07531 FvH4_2g08961 FvH4_2g25141 FvH4_2g27292 FvH4_3g18164 FvH4_3g19921 FvH4_3g43870 FvH4_4g14441 FvH4_4g15581 FvH4_4g15582 FvH4_4g16890 FvH4_4g19782 FvH4_4g33444 FvH4_4g33921 FvH4_5g22281 FvH4_5g22282 FvH4_5g32571 FvH4_5g37451 FvH4_5g39631 FvH4_6g04721 FvH4_6g11661 FvH4_6g20571 FvH4_6g32791 FvH4_7g23184
prunus_persica Prupe.1G090900_v2.0.a1 Prupe.1G241200_v2.0.a1 Prupe.1G534100_v2.0.a1 Prupe.3G076700_v2.0.a1 Prupe.4G150800_v2.0.a1 Prupe.4G181800_v2.0.a1 Prupe.4G240900_v2.0.a1 Prupe.4G247600_v2.0.a1 Prupe.6G115900_v2.0.a1 Prupe.6G327500_v2.0.a1 Prupe.8G071700_v2.0.a1
pyrus_communis pycom06g11480 pycom10g08330 pycom15g04320 pycom15g04350 pycom15g12620 pycom2874g00060
rosa_chinensis RchiOBHm_Chr1g0317241 RchiOBHm_Chr1g0341801 RchiOBHm_Chr1g0341931 RchiOBHm_Chr1g0359621 RchiOBHm_Chr2g0113741 RchiOBHm_Chr2g0117261 RchiOBHm_Chr2g0138831 RchiOBHm_Chr2g0141421 RchiOBHm_Chr2g0145281 RchiOBHm_Chr2g0152991 RchiOBHm_Chr3g0448001 RchiOBHm_Chr3g0450981 RchiOBHm_Chr3g0455161 RchiOBHm_Chr3g0457401 RchiOBHm_Chr3g0460001 RchiOBHm_Chr4g0387431 RchiOBHm_Chr4g0388741 RchiOBHm_Chr4g0444201 RchiOBHm_Chr4g0444261 RchiOBHm_Chr5g0010221 RchiOBHm_Chr6g0271721 RchiOBHm_Chr6g0274641 RchiOBHm_Chr6g0294691 RchiOBHm_Chr7g0237031 RchiOBHm_Chr7g0238461 RchiOBHm_Chr7g0239981
rosa_laevigata RLG00000009017
rosa_multiflora Rmu_co7986768.1_g000001 Rmu_co8302239.1_g000001 Rmu_co8485799.1_g000001 Rmu_sc0000061.1_g000013 Rmu_sc0000303.1_g000034 Rmu_sc0000303.1_g000035 Rmu_sc0000315.1_g000017 Rmu_sc0000349.1_g000003 Rmu_sc0000498.1_g000068 Rmu_sc0000508.1_g000014 Rmu_sc0000602.1_g000001 Rmu_sc0000633.1_g000002 Rmu_sc0000638.1_g000030 Rmu_sc0000756.1_g000016 Rmu_sc0000756.1_g000017 Rmu_sc0000785.1_g000037 Rmu_sc0000795.1_g000017 Rmu_sc0000796.1_g000030 Rmu_sc0000863.1_g000023 Rmu_sc0000920.1_g000021 Rmu_sc0000960.1_g000015 Rmu_sc0000982.1_g000102 Rmu_sc0001036.1_g000008 Rmu_sc0001036.1_g000009 Rmu_sc0001643.1_g000029 Rmu_sc0001687.1_g000026 Rmu_sc0001708.1_g000037 Rmu_sc0001768.1_g000015 Rmu_sc0001790.1_g000022 Rmu_sc0002180.1_g000007 Rmu_sc0002211.1_g000006 Rmu_sc0002270.1_g000010 Rmu_sc0002404.1_g000031 Rmu_sc0002858.1_g000016 Rmu_sc0003063.1_g000028 Rmu_sc0003730.1_g000017 Rmu_sc0004087.1_g000020 Rmu_sc0004618.1_g000048 Rmu_sc0004724.1_g000005 Rmu_sc0005167.1_g000036 Rmu_sc0005781.1_g000003 Rmu_sc0005878.1_g000012 Rmu_sc0006011.1_g000003 Rmu_sc0006343.1_g000011 Rmu_sc0006573.1_g000007 Rmu_sc0006599.1_g000007 Rmu_sc0006803.1_g000014 Rmu_sc0006836.1_g000002 Rmu_sc0006849.1_g000002 Rmu_sc0007109.1_g000009 Rmu_sc0009268.1_g000016 Rmu_sc0009618.1_g000017 Rmu_sc0009674.1_g000009 Rmu_sc0010244.1_g000009 Rmu_sc0011151.1_g000004 Rmu_sc0011356.1_g000001 Rmu_sc0011674.1_g000002 Rmu_sc0011778.1_g000016 Rmu_sc0012342.1_g000001 Rmu_sc0012467.1_g000002 Rmu_sc0012574.1_g000003 Rmu_sc0012913.1_g000005 Rmu_sc0013612.1_g000020 Rmu_sc0014824.1_g000001 Rmu_sc0017869.1_g000002 Rmu_sc0018085.1_g000003 Rmu_sc0024789.1_g000001 Rmu_sc0025106.1_g000013 Rmu_sc0034502.1_g000001 Rmu_sc0034547.1_g000001 Rmu_sc0035094.1_g000001 Rmu_sc0035095.1_g000001 Rmu_ssc0000100.1_g000027 Rmu_ssc0000244.1_g000005 Rmu_ssc0000371.1_g000029 Rmu_ssc0000381.1_g000004
rosa_roxburghii Rroxscaffold_1G00015480 Rroxscaffold_1G00023580 Rroxscaffold_1G00039340 Rroxscaffold_1G00047080 Rroxscaffold_2G00095820 Rroxscaffold_2G00125200 Rroxscaffold_2G00128330 Rroxscaffold_3G00270860
rosa_rugosa Rorug06G0120700
rosa_samantha Rh3BG173100 Rh6AG089600 Rh7AG488100 Rh7DG389600
rosa_wichuraiana Rw0G001150 Rw0G001990 Rw0G002010 Rw0G019540 Rw1G013730 Rw1G024110 Rw1G029190 Rw2G018850 Rw2G021090 Rw2G022260 Rw2G023180 Rw2G027280 Rw3G010190 Rw3G020660 Rw3G029380 Rw4G002390 Rw4G002690 Rw4G033260 Rw5G011960 Rw5G023110 Rw5G025250 Rw5G028950 Rw5G033740 Rw5G033950 Rw5G037900 Rw6G009640 Rw6G011620 Rw6G027720 Rw7G010680 Rw7G042070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2787
Acc65I GGTACC 1 cut(s) 3011
AccB1I GGYRCC 2 cut(s) 2824, 3011
AccBSI CCGCTC 5 cut(s) 349, 361, 373, 385, 397
AccI GTMKAC 4 cut(s) 499, 681, 1403, 1634
AccII CGCG 2 cut(s) 337, 2106
AclWI GGATC 5 cut(s) 1107, 2062, 2880, 2893, 3093
AcoI YGGCCR 2 cut(s) 2366, 2678
AcsI RAATTY 6 cut(s) 427, 929, 1147, 2475, 2725, 2968
AdeI CACNNNGTG 1 cut(s) 826
AfiI CCNNNNNNNGG 1 cut(s) 2914
AflIII ACRYGT 3 cut(s) 439, 809, 3022
AhlI ACTAGT 1 cut(s) 157
AjiI CACGTC 2 cut(s) 2600, 3023
AjnI CCWGG 1 cut(s) 2931
AjuI GAANNNNNNNTTGG 2 cut(s) 235, 267
AluBI AGCT 7 cut(s) 10, 120, 423, 1001, 2606, 2684, 3233
AluI AGCT 7 cut(s) 10, 120, 423, 1001, 2606, 2684, 3233
Alw21I GWGCWC 2 cut(s) 831, 3251
Alw26I GTCTC 3 cut(s) 701, 2171, 2419
Alw44I GTGCAC 1 cut(s) 3247
AlwI GGATC 5 cut(s) 1107, 2062, 2880, 2893, 3093
Ama87I CYCGRG 2 cut(s) 203, 2985
AoxI GGCC 6 cut(s) 127, 1409, 1461, 2366, 2678, 2896
ApaLI GTGCAC 1 cut(s) 3247
ApeKI GCWGC 9 cut(s) 7, 998, 1709, 1758, 1773, 1776, 1864, 2879, 2912
ApoI RAATTY 6 cut(s) 427, 929, 1147, 2475, 2725, 2968
ArsI GACNNNNNNTTYG 2 cut(s) 2984, 3016
AseI ATTAAT 2 cut(s) 1395, 1565
Asp700I GAANNNNTTC 2 cut(s) 1028, 2475
Asp718I GGTACC 1 cut(s) 3011
AspS9I GGNCC 5 cut(s) 127, 843, 1056, 2073, 3265
AsuHPI GGTGA 3 cut(s) 454, 2240, 3002
AvaI CYCGRG 2 cut(s) 203, 2985
AvaII GGWCC 4 cut(s) 843, 1056, 2073, 3265
BaeGI GKGCMC 1 cut(s) 3251
BaeI ACNNNNGTAYC 4 cut(s) 1679, 1712, 2995, 3028
BamHI GGATCC 1 cut(s) 2885
BanI GGYRCC 2 cut(s) 2824, 3011
Bbv12I GWGCWC 2 cut(s) 831, 3251
BbvCI CCTCAGC 1 cut(s) 2148
BbvI GCAGC 9 cut(s) 19, 1010, 1696, 1760, 1763, 1770, 1851, 2891, 2899
BccI CCATC 7 cut(s) 554, 2294, 2354, 2431, 2620, 3204, 3276
BceAI ACGGC 6 cut(s) 1659, 1764, 1826, 2641, 2883, 2924
BcgI CGANNNNNNTGC 2 cut(s) 1153, 1187
BciT130I CCWGG 1 cut(s) 2933
BciVI GTATCC 2 cut(s) 1277, 2794
BclI TGATCA 1 cut(s) 2208
BcoDI GTCTC 3 cut(s) 701, 2171, 2419
BcuI ACTAGT 1 cut(s) 157
BfaI CTAG 9 cut(s) 158, 534, 1446, 1608, 2154, 2700, 2946, 2952, 3216
BfmI CTRYAG 2 cut(s) 1351, 1657
BfuI GTATCC 2 cut(s) 1277, 2794
BglII AGATCT 2 cut(s) 229, 913
Bme1390I CCNGG 1 cut(s) 2933
Bme18I GGWCC 4 cut(s) 843, 1056, 2073, 3265
BmeT110I CYCGRG 2 cut(s) 203, 2985
BmgBI CACGTC 2 cut(s) 2600, 3023
BmgT120I GGNCC 5 cut(s) 127, 843, 1056, 2073, 3265
BmiI GGNNCC 8 cut(s) 128, 341, 631, 2142, 2826, 2887, 3013, 3266
BmrFI CCNGG 1 cut(s) 2933
BmrI ACTGGG 1 cut(s) 2136
BmsI GCATC 6 cut(s) 192, 1118, 1134, 1211, 2008, 2079
BmuI ACTGGG 1 cut(s) 2136
BplI GAGNNNNNCTC 2 cut(s) 614, 646
BpmI CTGGAG 1 cut(s) 2944
Bpu10I CCTNAGC 1 cut(s) 2148
BpuEI CTTGAG 4 cut(s) 818, 902, 1921, 2378
Bsa29I ATCGAT 1 cut(s) 1277
BsaAI YACGTR 4 cut(s) 1337, 2255, 2537, 2552
BsaBI GATNNNNATC 3 cut(s) 234, 541, 912
BsaI GGTCTC 1 cut(s) 2419
BsaJI CCNNGG 6 cut(s) 69, 335, 2076, 2661, 2865, 3015
Bsc4I CCNNNNNNNGG 1 cut(s) 2914
Bse118I RCCGGY 1 cut(s) 2224
Bse1I ACTGG 4 cut(s) 2131, 2369, 2889, 2961
Bse3DI GCAATG 4 cut(s) 138, 753, 2094, 2673
Bse8I GATNNNNATC 3 cut(s) 234, 541, 912
BseBI CCWGG 1 cut(s) 2933
BseCI ATCGAT 1 cut(s) 1277
BseDI CCNNGG 6 cut(s) 69, 335, 2076, 2661, 2865, 3015
BseGI GGATG 4 cut(s) 546, 913, 1202, 2521
BseJI GATNNNNATC 3 cut(s) 234, 541, 912
BseLI CCNNNNNNNGG 1 cut(s) 2914
BseMI GCAATG 4 cut(s) 138, 753, 2094, 2673
BseMII CTCAG 6 cut(s) 633, 2139, 2151, 2330, 2570, 2787
BseNI ACTGG 4 cut(s) 2131, 2369, 2889, 2961
BseRI GAGGAG 3 cut(s) 719, 821, 2591
BseSI GKGCMC 1 cut(s) 3251
BseXI GCAGC 9 cut(s) 19, 1010, 1696, 1760, 1763, 1770, 1851, 2891, 2899
BsgI GTGCAG 4 cut(s) 722, 1721, 1746, 1777
Bsh1236I CGCG 2 cut(s) 337, 2106
BshFI GGCC 6 cut(s) 129, 1411, 1463, 2368, 2680, 2898
BshNI GGYRCC 2 cut(s) 2824, 3011
BshVI ATCGAT 1 cut(s) 1277
BsiHKAI GWGCWC 2 cut(s) 831, 3251
BsiHKCI CYCGRG 2 cut(s) 203, 2985
BsiSI CCGG 2 cut(s) 2225, 2883
BslFI GGGAC 2 cut(s) 3033, 3278
BslI CCNNNNNNNGG 1 cut(s) 2914
BsmAI GTCTC 3 cut(s) 701, 2171, 2419
BsmFI GGGAC 2 cut(s) 3033, 3278
BsmI GAATGC 2 cut(s) 1233, 2191
BsnI GGCC 6 cut(s) 129, 1411, 1463, 2368, 2680, 2898
Bso31I GGTCTC 1 cut(s) 2419
BsoBI CYCGRG 2 cut(s) 203, 2985
Bsp1286I GDGCHC 2 cut(s) 831, 3251
Bsp1407I TGTACA 2 cut(s) 806, 1792
Bsp19I CCATGG 1 cut(s) 2661
BspANI GGCC 6 cut(s) 129, 1411, 1463, 2368, 2680, 2898
BspCNI CTCAG 6 cut(s) 632, 2140, 2152, 2329, 2569, 2788
BspDI ATCGAT 1 cut(s) 1277
BspFNI CGCG 2 cut(s) 337, 2106
BspHI TCATGA 1 cut(s) 37
BspLI GGNNCC 8 cut(s) 128, 341, 631, 2142, 2826, 2887, 3013, 3266
BspMAI CTGCAG 1 cut(s) 1661
BspPI GGATC 5 cut(s) 1107, 2062, 2880, 2893, 3093
BspT107I GGYRCC 2 cut(s) 2824, 3011
BspTNI GGTCTC 1 cut(s) 2419
BsrBI CCGCTC 5 cut(s) 349, 361, 373, 385, 397
BsrDI GCAATG 4 cut(s) 138, 753, 2094, 2673
BsrFI RCCGGY 1 cut(s) 2224
BsrGI TGTACA 2 cut(s) 806, 1792
BsrI ACTGG 4 cut(s) 2131, 2369, 2889, 2961
BssAI RCCGGY 1 cut(s) 2224
BssECI CCNNGG 6 cut(s) 69, 335, 2076, 2661, 2865, 3015
BssT1I CCWWGG 3 cut(s) 2076, 2661, 2865
Bst2UI CCWGG 1 cut(s) 2933
Bst4CI ACNGT 9 cut(s) 989, 1144, 1692, 1825, 1919, 2041, 2929, 3016, 3240
Bst6I CTCTTC 3 cut(s) 585, 933, 2271
BstAPI GCANNNNNTGC 1 cut(s) 2824
BstAUI TGTACA 2 cut(s) 806, 1792
BstBAI YACGTR 4 cut(s) 1337, 2255, 2537, 2552
BstC8I GCNNGC 8 cut(s) 181, 311, 335, 359, 383, 2145, 2682, 2862
BstDSI CCRYGG 3 cut(s) 335, 2661, 3015
BstEII GGTNACC 1 cut(s) 521
BstF5I GGATG 4 cut(s) 546, 913, 1202, 2521
BstFNI CGCG 2 cut(s) 337, 2106
BstMAI GTCTC 3 cut(s) 701, 2171, 2419
BstMWI GCNNNNNNNGC 5 cut(s) 126, 420, 2504, 2824, 2909
BstNI CCWGG 1 cut(s) 2933
BstNSI RCATGY 5 cut(s) 443, 745, 813, 2618, 2808
BstPI GGTNACC 1 cut(s) 521
BstSCI CCNGG 1 cut(s) 2931
BstSFI CTRYAG 2 cut(s) 1351, 1657
BstSLI GKGCMC 1 cut(s) 3251
BstSNI TACGTA 3 cut(s) 2255, 2537, 2552
BstUI CGCG 2 cut(s) 337, 2106
BstV1I GCAGC 9 cut(s) 19, 1010, 1696, 1760, 1763, 1770, 1851, 2891, 2899
BstX2I RGATCY 3 cut(s) 229, 913, 2885
BstXI CCANNNNNNTGG 1 cut(s) 2083
BstYI RGATCY 3 cut(s) 229, 913, 2885
Bsu15I ATCGAT 1 cut(s) 1277
BsuI GTATCC 2 cut(s) 1277, 2794
BsuRI GGCC 6 cut(s) 129, 1411, 1463, 2368, 2680, 2898
BsuTUI ATCGAT 1 cut(s) 1277
BtgI CCRYGG 3 cut(s) 335, 2661, 3015
BtrI CACGTC 2 cut(s) 2600, 3023
BtsCI GGATG 4 cut(s) 546, 913, 1202, 2521
BtsI GCAGTG 5 cut(s) 328, 352, 376, 1734, 1951
BtsIMutI CAGTG 6 cut(s) 328, 352, 376, 1734, 1951, 2896
Cac8I GCNNGC 8 cut(s) 181, 311, 335, 359, 383, 2145, 2682, 2862
CciI TCATGA 1 cut(s) 37
Cfr10I RCCGGY 1 cut(s) 2224
Cfr13I GGNCC 5 cut(s) 127, 843, 1056, 2073, 3265
Cfr42I CCGCGG 1 cut(s) 338
ClaI ATCGAT 1 cut(s) 1277
CseI GACGC 2 cut(s) 2010, 2112
CspCI CAANNNNNGTGG 2 cut(s) 2854, 2889
DraI TTTAAA 2 cut(s) 1240, 2724
DraIII CACNNNGTG 1 cut(s) 826
DrdI GACNNNNNNGTC 1 cut(s) 2787
DseDI GACNNNNNNGTC 1 cut(s) 2787
EaeI YGGCCR 2 cut(s) 2366, 2678
Eam1104I CTCTTC 3 cut(s) 585, 933, 2271
EarI CTCTTC 3 cut(s) 585, 933, 2271
Eco105I TACGTA 3 cut(s) 2255, 2537, 2552
Eco130I CCWWGG 3 cut(s) 2076, 2661, 2865
Eco31I GGTCTC 1 cut(s) 2419
Eco47I GGWCC 4 cut(s) 843, 1056, 2073, 3265
Eco88I CYCGRG 2 cut(s) 203, 2985
Eco91I GGTNACC 1 cut(s) 521
EcoO109I RGGNCCY 1 cut(s) 127
EcoO65I GGTNACC 1 cut(s) 521
EcoRII CCWGG 1 cut(s) 2931
EcoT14I CCWWGG 3 cut(s) 2076, 2661, 2865
EcoT22I ATGCAT 1 cut(s) 747
ErhI CCWWGG 3 cut(s) 2076, 2661, 2865
FaqI GGGAC 2 cut(s) 3033, 3278
FauI CCCGC 8 cut(s) 318, 342, 354, 366, 378, 390, 402, 2500
FauNDI CATATG 2 cut(s) 3090, 3097
FbaI TGATCA 1 cut(s) 2208
FblI GTMKAC 4 cut(s) 499, 681, 1403, 1634
FokI GGATG 4 cut(s) 533, 920, 1189, 2528
FspBI CTAG 9 cut(s) 158, 534, 1446, 1608, 2154, 2700, 2946, 2952, 3216
GsuI CTGGAG 1 cut(s) 2944
HaeIII GGCC 6 cut(s) 129, 1411, 1463, 2368, 2680, 2898
HapII CCGG 2 cut(s) 2225, 2883
HgaI GACGC 2 cut(s) 2010, 2112
HincII GTYRAC 1 cut(s) 1635
HindII GTYRAC 1 cut(s) 1635
HindIII AAGCTT 1 cut(s) 421
HpaII CCGG 2 cut(s) 2225, 2883
HphI GGTGA 3 cut(s) 454, 2240, 3002
Hpy99I CGWCG 1 cut(s) 2601
HpyAV CCTTC 5 cut(s) 1171, 1474, 2107, 2326, 2577
HpyCH4III ACNGT 9 cut(s) 989, 1144, 1692, 1825, 1919, 2041, 2929, 3016, 3240
HpyCH4IV ACGT 8 cut(s) 42, 1336, 2254, 2536, 2551, 2599, 3022, 3310
HpyF10VI GCNNNNNNNGC 5 cut(s) 126, 420, 2504, 2824, 2909
HpySE526I ACGT 8 cut(s) 42, 1336, 2254, 2536, 2551, 2599, 3022, 3310
KpnI GGTACC 1 cut(s) 3015
Ksp22I TGATCA 1 cut(s) 2208
KspI CCGCGG 1 cut(s) 338
LmnI GCTCC 7 cut(s) 354, 378, 635, 834, 2140, 2869, 2876
Lsp1109I GCAGC 9 cut(s) 19, 1010, 1696, 1760, 1763, 1770, 1851, 2891, 2899
LweI GCATC 6 cut(s) 192, 1118, 1134, 1211, 2008, 2079
MaeI CTAG 9 cut(s) 158, 534, 1446, 1608, 2154, 2700, 2946, 2952, 3216
MaeII ACGT 8 cut(s) 42, 1336, 2254, 2536, 2551, 2599, 3022, 3310
MaeIII GTNAC 9 cut(s) 64, 521, 1874, 2122, 2636, 2954, 2990, 3279, 3306
MbiI CCGCTC 5 cut(s) 349, 361, 373, 385, 397
MfeI CAATTG 3 cut(s) 250, 1816, 1910
MflI RGATCY 3 cut(s) 229, 913, 2885
MhlI GDGCHC 2 cut(s) 831, 3251
MlyI GAGTC 6 cut(s) 1351, 1516, 1928, 2343, 2987, 3065
MmeI TCCRAC 2 cut(s) 554, 3041
Mph1103I ATGCAT 1 cut(s) 747
MroXI GAANNNNTTC 2 cut(s) 1028, 2475
MslI CAYNNNNRTG 4 cut(s) 143, 2666, 3254, 3331
MspA1I CMGCKG 4 cut(s) 313, 337, 1623, 2684
MspI CCGG 2 cut(s) 2225, 2883
MspR9I CCNGG 1 cut(s) 2933
MunI CAATTG 3 cut(s) 250, 1816, 1910
Mva1269I GAATGC 2 cut(s) 1233, 2191
MvaI CCWGG 1 cut(s) 2933
MvnI CGCG 2 cut(s) 337, 2106
MwoI GCNNNNNNNGC 5 cut(s) 126, 420, 2504, 2824, 2909
NcoI CCATGG 1 cut(s) 2661
NdeI CATATG 2 cut(s) 3090, 3097
NlaIV GGNNCC 8 cut(s) 128, 341, 631, 2142, 2826, 2887, 3013, 3266
NmeAIII GCCGAG 1 cut(s) 2656
NmuCI GTSAC 3 cut(s) 64, 2122, 2990
NsiI ATGCAT 1 cut(s) 747
NspI RCATGY 5 cut(s) 443, 745, 813, 2618, 2808
PaeR7I CTCGAG 1 cut(s) 203
PagI TCATGA 1 cut(s) 37
PciI ACATGT 2 cut(s) 439, 809
PctI GAATGC 2 cut(s) 1233, 2191
PdmI GAANNNNTTC 2 cut(s) 1028, 2475
PfeI GAWTC 7 cut(s) 529, 537, 562, 922, 946, 1996, 3064
PflFI GACNNNGTC 1 cut(s) 2352
PfoI TCCNGGA 1 cut(s) 2931
PleI GAGTC 6 cut(s) 1351, 1515, 1928, 2343, 2987, 3065
PpsI GAGTC 6 cut(s) 1351, 1515, 1928, 2343, 2987, 3065
Ppu21I YACGTR 4 cut(s) 1337, 2255, 2537, 2552
PscI ACATGT 2 cut(s) 439, 809
PshBI ATTAAT 2 cut(s) 1395, 1565
Psp6I CCWGG 1 cut(s) 2931
PspEI GGTNACC 1 cut(s) 521
PspGI CCWGG 1 cut(s) 2931
PspN4I GGNNCC 8 cut(s) 128, 341, 631, 2142, 2826, 2887, 3013, 3266
PspPI GGNCC 5 cut(s) 127, 843, 1056, 2073, 3265
PstI CTGCAG 1 cut(s) 1661
PsuI RGATCY 3 cut(s) 229, 913, 2885
PsyI GACNNNGTC 1 cut(s) 2352
PvuII CAGCTG 1 cut(s) 2684
RseI CAYNNNNRTG 4 cut(s) 143, 2666, 3254, 3331
SacII CCGCGG 1 cut(s) 338
SalI GTCGAC 1 cut(s) 1633
Sau96I GGNCC 5 cut(s) 127, 843, 1056, 2073, 3265
SchI GAGTC 6 cut(s) 1351, 1516, 1928, 2343, 2987, 3065
ScrFI CCNGG 1 cut(s) 2933
SduI GDGCHC 2 cut(s) 831, 3251
SfaNI GCATC 6 cut(s) 192, 1118, 1134, 1211, 2008, 2079
SfcI CTRYAG 2 cut(s) 1351, 1657
Sfr274I CTCGAG 1 cut(s) 203
Sfr303I CCGCGG 1 cut(s) 338
SgrBI CCGCGG 1 cut(s) 338
SinI GGWCC 4 cut(s) 843, 1056, 2073, 3265
SlaI CTCGAG 1 cut(s) 203
SmiMI CAYNNNNRTG 4 cut(s) 143, 2666, 3254, 3331
SmlI CTYRAG 5 cut(s) 203, 833, 917, 1936, 2393
SmoI CTYRAG 5 cut(s) 203, 833, 917, 1936, 2393
SnaBI TACGTA 3 cut(s) 2255, 2537, 2552
SpeI ACTAGT 1 cut(s) 157
SspI AATATT 1 cut(s) 1476
SspMI CTAG 9 cut(s) 158, 534, 1446, 1608, 2154, 2700, 2946, 2952, 3216
StyD4I CCNGG 1 cut(s) 2931
StyI CCWWGG 3 cut(s) 2076, 2661, 2865
TaaI ACNGT 9 cut(s) 989, 1144, 1692, 1825, 1919, 2041, 2929, 3016, 3240
TaiI ACGT 8 cut(s) 45, 1339, 2257, 2539, 2554, 2602, 3025, 3313
TaqII GACCGA 1 cut(s) 83
TatI WGTACW 4 cut(s) 806, 1792, 1853, 2768
TauI GCSGC 1 cut(s) 2918
TfiI GAWTC 7 cut(s) 529, 537, 562, 922, 946, 1996, 3064
TscAI CASTG 6 cut(s) 335, 359, 383, 1734, 1951, 2896
TseFI GTSAC 3 cut(s) 64, 2122, 2990
TseI GCWGC 9 cut(s) 7, 998, 1709, 1758, 1773, 1776, 1864, 2879, 2912
Tsp45I GTSAC 3 cut(s) 64, 2122, 2990
TspGWI ACGGA 4 cut(s) 2342, 2389, 2437, 3098
TspRI CASTG 6 cut(s) 335, 359, 383, 1734, 1951, 2896
Tth111I GACNNNGTC 1 cut(s) 2352
VneI GTGCAC 1 cut(s) 3247
VpaK11BI GGWCC 4 cut(s) 843, 1056, 2073, 3265
VspI ATTAAT 2 cut(s) 1395, 1565
XapI RAATTY 6 cut(s) 427, 929, 1147, 2475, 2725, 2968
XceI RCATGY 5 cut(s) 443, 745, 813, 2618, 2808
XhoI CTCGAG 1 cut(s) 203
XmiI GTMKAC 4 cut(s) 499, 681, 1403, 1634
XmnI GAANNNNTTC 2 cut(s) 1028, 2475
XspI CTAG 9 cut(s) 158, 534, 1446, 1608, 2154, 2700, 2946, 2952, 3216
Zsp2I ATGCAT 1 cut(s) 747
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.