Rw0G019540
RLK Family

Cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00878
OG0000146
Physical Location & Seq
Reverse (-)
42994 .. 43887
894 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G019540.1

Sequence Viewer

Length: 855 bp
ATGGTTCTCGATGAATTACACACTGATTCAACCCACACATCAGCAACCAACTCCGAAGCTTCTTCTCAATATAAAGGGCGATCGTCATCCAATGATCATGGCCCTATTGCTCCAGAAAAATTGGATAGCACCAATTTTATTTCTTGGTCTAAACATGCCAAGTTGAACATCACTGGCCGAGGCAAACTTGGCTATCTCACTGGCAAAAAGAAATCACCATTTGAAGAAGAAGATGATGAGGCATGGCTCCTTAACTCTATGCACAAGGATGTAAGAGGAAATTATGAACGCTTAGAGACAGCTAAAGAAATTTGGGATGCTGTCAAAACTACTCCCAAGATGATACAAGAAACTATGAACTGCAAATTGCTTCTGTTTTCACTAAACAGAATGGAGCTCTTCTTTATGGTTATTACAACAATATATATGAAGAAGATAAGGCAGGAGCTTGACATATTGGATCCTCTCCGGCTCAAGGATGCAGATTTTATCACCTACATGGATAATAGGGTCACTAAGCACCGTGTCTATTTCTTTTTGGCCAGGCTAGACCCTCACCTTGATGGAGTCCGGAGTAGAATCCTAAACACCAAGCCGTTGCCTTCCATAGAAGAGGCTTATTCTCAAGTCAGTGCTGAGCATAATAGACTCCAAAAGATGATGTCCGACGCAAAGTACGAAGGATCTGCCTTGGTTGCTCAATACCGACCACCGCATGTCTGTAGCTTCCAGCCTTCTTTATCTACATCACTTTGGAAAGTACCCTCTCCAGTACCCATGAAATTCACTCATTGCAAATCAACCGGGCATATGGTAGAGACTTGTTCTCAATTACATGGCTTTCCAGACTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

284

Amino Acids

32.63

Weight (kDa)

8.23

Isoelectric Point (pI)

44.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotran_gag_3 PF14244 35 - 73 8.7e-06 gag-polypeptide of LTR copia-type
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000146)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19561 FvH4_1g25371 FvH4_1g25961 FvH4_2g00352 FvH4_2g00861 FvH4_2g04952 FvH4_2g05413 FvH4_2g07531 FvH4_2g08961 FvH4_2g25141 FvH4_2g27292 FvH4_3g18164 FvH4_3g19921 FvH4_3g43870 FvH4_4g14441 FvH4_4g15581 FvH4_4g15582 FvH4_4g16890 FvH4_4g19782 FvH4_4g33444 FvH4_4g33921 FvH4_5g22281 FvH4_5g22282 FvH4_5g32571 FvH4_5g37451 FvH4_5g39631 FvH4_6g04721 FvH4_6g11661 FvH4_6g20571 FvH4_6g32791 FvH4_7g23184
prunus_persica Prupe.1G090900_v2.0.a1 Prupe.1G241200_v2.0.a1 Prupe.1G534100_v2.0.a1 Prupe.3G076700_v2.0.a1 Prupe.4G150800_v2.0.a1 Prupe.4G181800_v2.0.a1 Prupe.4G240900_v2.0.a1 Prupe.4G247600_v2.0.a1 Prupe.6G115900_v2.0.a1 Prupe.6G327500_v2.0.a1 Prupe.8G071700_v2.0.a1
pyrus_communis pycom06g11480 pycom10g08330 pycom15g04320 pycom15g04350 pycom15g12620 pycom2874g00060
rosa_chinensis RchiOBHm_Chr1g0317241 RchiOBHm_Chr1g0341801 RchiOBHm_Chr1g0341931 RchiOBHm_Chr1g0359621 RchiOBHm_Chr2g0113741 RchiOBHm_Chr2g0117261 RchiOBHm_Chr2g0138831 RchiOBHm_Chr2g0141421 RchiOBHm_Chr2g0145281 RchiOBHm_Chr2g0152991 RchiOBHm_Chr3g0448001 RchiOBHm_Chr3g0450981 RchiOBHm_Chr3g0455161 RchiOBHm_Chr3g0457401 RchiOBHm_Chr3g0460001 RchiOBHm_Chr4g0387431 RchiOBHm_Chr4g0388741 RchiOBHm_Chr4g0444201 RchiOBHm_Chr4g0444261 RchiOBHm_Chr5g0010221 RchiOBHm_Chr6g0271721 RchiOBHm_Chr6g0274641 RchiOBHm_Chr6g0294691 RchiOBHm_Chr7g0237031 RchiOBHm_Chr7g0238461 RchiOBHm_Chr7g0239981
rosa_laevigata RLG00000009017
rosa_multiflora Rmu_co7986768.1_g000001 Rmu_co8302239.1_g000001 Rmu_co8485799.1_g000001 Rmu_sc0000061.1_g000013 Rmu_sc0000303.1_g000034 Rmu_sc0000303.1_g000035 Rmu_sc0000315.1_g000017 Rmu_sc0000349.1_g000003 Rmu_sc0000498.1_g000068 Rmu_sc0000508.1_g000014 Rmu_sc0000602.1_g000001 Rmu_sc0000633.1_g000002 Rmu_sc0000638.1_g000030 Rmu_sc0000756.1_g000016 Rmu_sc0000756.1_g000017 Rmu_sc0000785.1_g000037 Rmu_sc0000795.1_g000017 Rmu_sc0000796.1_g000030 Rmu_sc0000863.1_g000023 Rmu_sc0000920.1_g000021 Rmu_sc0000960.1_g000015 Rmu_sc0000982.1_g000102 Rmu_sc0001036.1_g000008 Rmu_sc0001036.1_g000009 Rmu_sc0001643.1_g000029 Rmu_sc0001687.1_g000026 Rmu_sc0001708.1_g000037 Rmu_sc0001768.1_g000015 Rmu_sc0001790.1_g000022 Rmu_sc0002180.1_g000007 Rmu_sc0002211.1_g000006 Rmu_sc0002270.1_g000010 Rmu_sc0002404.1_g000031 Rmu_sc0002858.1_g000016 Rmu_sc0003063.1_g000028 Rmu_sc0003730.1_g000017 Rmu_sc0004087.1_g000020 Rmu_sc0004618.1_g000048 Rmu_sc0004724.1_g000005 Rmu_sc0005167.1_g000036 Rmu_sc0005781.1_g000003 Rmu_sc0005878.1_g000012 Rmu_sc0006011.1_g000003 Rmu_sc0006343.1_g000011 Rmu_sc0006573.1_g000007 Rmu_sc0006599.1_g000007 Rmu_sc0006803.1_g000014 Rmu_sc0006836.1_g000002 Rmu_sc0006849.1_g000002 Rmu_sc0007109.1_g000009 Rmu_sc0009268.1_g000016 Rmu_sc0009618.1_g000017 Rmu_sc0009674.1_g000009 Rmu_sc0010244.1_g000009 Rmu_sc0011151.1_g000004 Rmu_sc0011356.1_g000001 Rmu_sc0011674.1_g000002 Rmu_sc0011778.1_g000016 Rmu_sc0012342.1_g000001 Rmu_sc0012467.1_g000002 Rmu_sc0012574.1_g000003 Rmu_sc0012913.1_g000005 Rmu_sc0013612.1_g000020 Rmu_sc0014824.1_g000001 Rmu_sc0017869.1_g000002 Rmu_sc0018085.1_g000003 Rmu_sc0024789.1_g000001 Rmu_sc0025106.1_g000013 Rmu_sc0034502.1_g000001 Rmu_sc0034547.1_g000001 Rmu_sc0035094.1_g000001 Rmu_sc0035095.1_g000001 Rmu_ssc0000100.1_g000027 Rmu_ssc0000244.1_g000005 Rmu_ssc0000371.1_g000029 Rmu_ssc0000381.1_g000004
rosa_roxburghii Rroxscaffold_1G00015480 Rroxscaffold_1G00023580 Rroxscaffold_1G00039340 Rroxscaffold_1G00047080 Rroxscaffold_2G00095820 Rroxscaffold_2G00125200 Rroxscaffold_2G00128330 Rroxscaffold_3G00270860
rosa_rugosa Rorug06G0120700
rosa_samantha Rh3BG173100 Rh6AG089600 Rh7AG488100 Rh7DG389600
rosa_wichuraiana Rw0G001150 Rw0G001990 Rw0G002010 Rw0G019540 Rw1G013730 Rw1G024110 Rw1G029190 Rw2G018850 Rw2G021090 Rw2G022260 Rw2G023180 Rw2G027280 Rw3G010190 Rw3G020660 Rw3G029380 Rw4G002390 Rw4G002690 Rw4G033260 Rw5G011960 Rw5G023110 Rw5G025250 Rw5G028950 Rw5G033740 Rw5G033950 Rw5G037900 Rw6G009640 Rw6G011620 Rw6G027720 Rw7G010680 Rw7G042070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 570
AciI CCGC 1 cut(s) 713
AclWI GGATC 3 cut(s) 455, 468, 691
AcoI YGGCCR 2 cut(s) 175, 540
AcsI RAATTY 2 cut(s) 309, 782
AfaI GTAC 3 cut(s) 677, 762, 774
AfiI CCNNNNNNNGG 1 cut(s) 475
AgsI TTSAA 3 cut(s) 30, 166, 224
AjnI CCWGG 1 cut(s) 542
AluBI AGCT 5 cut(s) 59, 302, 397, 448, 726
AluI AGCT 5 cut(s) 59, 302, 397, 448, 726
Alw21I GWGCWC 1 cut(s) 399
Alw26I GTCTC 2 cut(s) 290, 812
AlwI GGATC 3 cut(s) 455, 468, 691
Aor13HI TCCGGA 1 cut(s) 570
AoxI GGCC 3 cut(s) 100, 175, 540
ApoI RAATTY 2 cut(s) 309, 782
AspS9I GGNCC 1 cut(s) 101
AsuC2I CCSGG 1 cut(s) 805
AsuHPI GGTGA 3 cut(s) 207, 484, 548
BalI TGGCCA 1 cut(s) 542
BamHI GGATCC 1 cut(s) 460
BanII GRGCYC 1 cut(s) 399
Bbv12I GWGCWC 1 cut(s) 399
BccI CCATC 1 cut(s) 557
BceAI ACGGC 1 cut(s) 580
BcgI CGANNNNNNTGC 2 cut(s) 668, 702
BciT130I CCWGG 1 cut(s) 544
BclI TGATCA 1 cut(s) 94
BcnI CCSGG 1 cut(s) 805
BcoDI GTCTC 2 cut(s) 290, 812
BfaI CTAG 1 cut(s) 548
BfmI CTRYAG 1 cut(s) 721
BlpI GCTNAGC 1 cut(s) 636
Bme1390I CCNGG 2 cut(s) 544, 805
BmgT120I GGNCC 1 cut(s) 101
BmiI GGNNCC 2 cut(s) 248, 462
BmrFI CCNGG 2 cut(s) 544, 805
BmsI GCATC 2 cut(s) 307, 469
BpmI CTGGAG 2 cut(s) 96, 753
Bpu1102I GCTNAGC 1 cut(s) 636
BpuEI CTTGAG 2 cut(s) 458, 609
BpuMI CCSGG 1 cut(s) 805
BsaBI GATNNNNATC 1 cut(s) 85
BsaJI CCNNGG 2 cut(s) 178, 690
BsaWI WCCGGW 1 cut(s) 570
Bsc4I CCNNNNNNNGG 1 cut(s) 475
Bse1I ACTGG 4 cut(s) 178, 205, 770, 854
Bse3DI GCAATG 1 cut(s) 790
Bse8I GATNNNNATC 1 cut(s) 85
BseAI TCCGGA 1 cut(s) 570
BseBI CCWGG 1 cut(s) 544
BseDI CCNNGG 2 cut(s) 178, 690
BseGI GGATG 4 cut(s) 86, 274, 322, 484
BseJI GATNNNNATC 1 cut(s) 85
BseLI CCNNNNNNNGG 1 cut(s) 475
BseMI GCAATG 1 cut(s) 790
BseMII CTCAG 1 cut(s) 627
BseNI ACTGG 4 cut(s) 178, 205, 770, 854
Bsh1285I CGRYCG 1 cut(s) 83
BshFI GGCC 3 cut(s) 102, 177, 542
BsiEI CGRYCG 1 cut(s) 83
BsiHKAI GWGCWC 1 cut(s) 399
BsiSI CCGG 3 cut(s) 469, 571, 804
BslI CCNNNNNNNGG 1 cut(s) 475
BsmAI GTCTC 2 cut(s) 290, 812
BsnI GGCC 3 cut(s) 102, 177, 542
Bsp1286I GDGCHC 1 cut(s) 399
Bsp13I TCCGGA 1 cut(s) 570
Bsp143I GATC 4 cut(s) 80, 94, 460, 683
Bsp1720I GCTNAGC 1 cut(s) 636
BspACI CCGC 1 cut(s) 713
BspANI GGCC 3 cut(s) 102, 177, 542
BspCNI CTCAG 1 cut(s) 628
BspEI TCCGGA 1 cut(s) 570
BspLI GGNNCC 2 cut(s) 248, 462
BspPI GGATC 3 cut(s) 455, 468, 691
BspQI GCTCTTC 1 cut(s) 404
BsrDI GCAATG 1 cut(s) 790
BsrI ACTGG 4 cut(s) 178, 205, 770, 854
BssECI CCNNGG 2 cut(s) 178, 690
BssMI GATC 4 cut(s) 80, 94, 460, 683
BssT1I CCWWGG 1 cut(s) 690
Bst2UI CCWGG 1 cut(s) 544
Bst4CI ACNGT 1 cut(s) 524
Bst6I CTCTTC 2 cut(s) 404, 606
BstDEI CTNAG 3 cut(s) 292, 516, 636
BstF5I GGATG 4 cut(s) 86, 274, 322, 484
BstKTI GATC 4 cut(s) 83, 97, 463, 686
BstMAI GTCTC 2 cut(s) 290, 812
BstMBI GATC 4 cut(s) 80, 94, 460, 683
BstMCI CGRYCG 1 cut(s) 83
BstMWI GCNNNNNNNGC 2 cut(s) 189, 695
BstNI CCWGG 1 cut(s) 544
BstNSI RCATGY 2 cut(s) 158, 719
BstSCI CCNGG 2 cut(s) 542, 803
BstSFI CTRYAG 1 cut(s) 721
BstX2I RGATCY 2 cut(s) 460, 683
BstYI RGATCY 2 cut(s) 460, 683
BsuRI GGCC 3 cut(s) 102, 177, 542
BtsCI GGATG 4 cut(s) 86, 274, 322, 484
BtsIMutI CAGTG 4 cut(s) 21, 171, 198, 637
Cfr13I GGNCC 1 cut(s) 101
CseI GACGC 1 cut(s) 677
Csp6I GTAC 3 cut(s) 676, 761, 773
CviAII CATG 7 cut(s) 98, 155, 243, 499, 716, 778, 836
CviQI GTAC 3 cut(s) 676, 761, 773
DdeI CTNAG 3 cut(s) 292, 516, 636
DpnI GATC 4 cut(s) 82, 96, 462, 685
DpnII GATC 4 cut(s) 80, 94, 460, 683
EaeI YGGCCR 2 cut(s) 175, 540
Eam1104I CTCTTC 2 cut(s) 404, 606
EarI CTCTTC 2 cut(s) 404, 606
Ecl136II GAGCTC 1 cut(s) 397
Eco130I CCWWGG 1 cut(s) 690
Eco24I GRGCYC 1 cut(s) 399
Eco53kI GAGCTC 1 cut(s) 397
EcoICRI GAGCTC 1 cut(s) 397
EcoRII CCWGG 1 cut(s) 542
EcoT14I CCWWGG 1 cut(s) 690
EcoT38I GRGCYC 1 cut(s) 399
ErhI CCWWGG 1 cut(s) 690
FaeI CATG 7 cut(s) 101, 158, 246, 502, 719, 781, 839
FatI CATG 7 cut(s) 97, 154, 242, 498, 715, 777, 835
FauNDI CATATG 1 cut(s) 810
FbaI TGATCA 1 cut(s) 94
FokI GGATG 4 cut(s) 73, 281, 329, 491
FriOI GRGCYC 1 cut(s) 399
FspBI CTAG 1 cut(s) 548
GsuI CTGGAG 2 cut(s) 96, 753
HaeIII GGCC 3 cut(s) 102, 177, 542
HapII CCGG 3 cut(s) 469, 571, 804
HgaI GACGC 1 cut(s) 677
Hin1II CATG 7 cut(s) 101, 158, 246, 502, 719, 781, 839
HindIII AAGCTT 1 cut(s) 57
HinfI GANTC 4 cut(s) 26, 567, 579, 648
HpaII CCGG 3 cut(s) 469, 571, 804
HphI GGTGA 3 cut(s) 207, 484, 548
Hpy188I TCNGA 2 cut(s) 55, 667
Hpy188III TCNNGA 4 cut(s) 8, 113, 571, 845
Hpy99I CGWCG 1 cut(s) 671
HpyAV CCTTC 3 cut(s) 612, 674, 744
HpyCH4III ACNGT 1 cut(s) 524
HpyCH4V TGCA 4 cut(s) 262, 363, 482, 795
HpyF10VI GCNNNNNNNGC 2 cut(s) 189, 695
HpyF3I CTNAG 3 cut(s) 292, 516, 636
Hsp92II CATG 7 cut(s) 101, 158, 246, 502, 719, 781, 839
Kpn2I TCCGGA 1 cut(s) 570
Ksp22I TGATCA 1 cut(s) 94
Kzo9I GATC 4 cut(s) 80, 94, 460, 683
LguI GCTCTTC 1 cut(s) 404
LmnI GCTCC 4 cut(s) 115, 252, 394, 445
LweI GCATC 2 cut(s) 307, 469
MaeI CTAG 1 cut(s) 548
MaeIII GTNAC 1 cut(s) 511
MalI GATC 4 cut(s) 82, 96, 462, 685
MboI GATC 4 cut(s) 80, 94, 460, 683
MboII GAAGA 8 cut(s) 54, 236, 239, 242, 391, 442, 445, 623
MflI RGATCY 2 cut(s) 460, 683
MhlI GDGCHC 1 cut(s) 399
MlsI TGGCCA 1 cut(s) 542
MluCI AATT 8 cut(s) 14, 119, 133, 280, 309, 365, 782, 830
MluNI TGGCCA 1 cut(s) 542
MlyI GAGTC 2 cut(s) 576, 642
MmeI TCCRAC 1 cut(s) 690
MnlI CCTC 7 cut(s) 173, 232, 269, 474, 564, 607, 775
Mox20I TGGCCA 1 cut(s) 542
MroI TCCGGA 1 cut(s) 570
MscI TGGCCA 1 cut(s) 542
MseI TTAA 1 cut(s) 252
MslI CAYNNNNRTG 3 cut(s) 267, 497, 561
Msp20I TGGCCA 1 cut(s) 542
MspI CCGG 3 cut(s) 469, 571, 804
MspR9I CCNGG 2 cut(s) 544, 805
MvaI CCWGG 1 cut(s) 544
MwoI GCNNNNNNNGC 2 cut(s) 189, 695
NciI CCSGG 1 cut(s) 805
NdeI CATATG 1 cut(s) 810
NdeII GATC 4 cut(s) 80, 94, 460, 683
NlaIII CATG 7 cut(s) 101, 158, 246, 502, 719, 781, 839
NlaIV GGNNCC 2 cut(s) 248, 462
NmeAIII GCCGAG 1 cut(s) 203
NmuCI GTSAC 1 cut(s) 511
NspI RCATGY 2 cut(s) 158, 719
PciSI GCTCTTC 1 cut(s) 404
PcsI WCGNNNNNNNCGW 1 cut(s) 675
PfeI GAWTC 2 cut(s) 26, 579
Ple19I CGATCG 1 cut(s) 83
PleI GAGTC 2 cut(s) 575, 642
PpsI GAGTC 2 cut(s) 575, 642
Psp124BI GAGCTC 1 cut(s) 399
Psp6I CCWGG 1 cut(s) 542
PspGI CCWGG 1 cut(s) 542
PspN4I GGNNCC 2 cut(s) 248, 462
PspPI GGNCC 1 cut(s) 101
PsuI RGATCY 2 cut(s) 460, 683
PvuI CGATCG 1 cut(s) 83
RsaI GTAC 3 cut(s) 677, 762, 774
RsaNI GTAC 3 cut(s) 676, 761, 773
RseI CAYNNNNRTG 3 cut(s) 267, 497, 561
SacI GAGCTC 1 cut(s) 399
SapI GCTCTTC 1 cut(s) 404
SaqAI TTAA 1 cut(s) 252
Sau3AI GATC 4 cut(s) 80, 94, 460, 683
Sau96I GGNCC 1 cut(s) 101
SchI GAGTC 2 cut(s) 576, 642
ScrFI CCNGG 2 cut(s) 544, 805
SduI GDGCHC 1 cut(s) 399
SetI ASST 7 cut(s) 61, 304, 399, 450, 497, 561, 728
SfaNI GCATC 2 cut(s) 307, 469
SfcI CTRYAG 1 cut(s) 721
SmiMI CAYNNNNRTG 3 cut(s) 267, 497, 561
SmlI CTYRAG 2 cut(s) 473, 624
SmoI CTYRAG 2 cut(s) 473, 624
Sse9I AATT 8 cut(s) 14, 119, 133, 280, 309, 365, 782, 830
SsiI CCGC 1 cut(s) 713
SspMI CTAG 1 cut(s) 548
SstI GAGCTC 1 cut(s) 399
StyD4I CCNGG 2 cut(s) 542, 803
StyI CCWWGG 1 cut(s) 690
TaaI ACNGT 1 cut(s) 524
TaqI TCGA 1 cut(s) 9
TasI AATT 8 cut(s) 14, 119, 133, 280, 309, 365, 782, 830
TfiI GAWTC 2 cut(s) 26, 579
Tru1I TTAA 1 cut(s) 252
Tru9I TTAA 1 cut(s) 252
TscAI CASTG 4 cut(s) 28, 178, 205, 637
TseFI GTSAC 1 cut(s) 511
Tsp45I GTSAC 1 cut(s) 511
TspDTI ATGAA 5 cut(s) 27, 300, 371, 443, 794
TspRI CASTG 4 cut(s) 28, 178, 205, 637
XapI RAATTY 2 cut(s) 309, 782
XceI RCATGY 2 cut(s) 158, 719
XspI CTAG 1 cut(s) 548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.