Belongs to the RecA family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1433 |
| AbsI | CCTCGAGG | 1 cut(s) | 1170 |
| Acc36I | ACCTGC | 1 cut(s) | 2021 |
| Acc65I | GGTACC | 1 cut(s) | 635 |
| AccB1I | GGYRCC | 2 cut(s) | 635, 646 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 619, 995 |
| AccI | GTMKAC | 1 cut(s) | 1779 |
| AccIII | TCCGGA | 1 cut(s) | 514 |
| AciI | CCGC | 2 cut(s) | 156, 1812 |
| AclWI | GGATC | 2 cut(s) | 1623, 1826 |
| AcsI | RAATTY | 3 cut(s) | 205, 1207, 1763 |
| AcuI | CTGAAG | 3 cut(s) | 32, 1251, 1977 |
| AcyI | GRCGYC | 1 cut(s) | 647 |
| AfaI | GTAC | 7 cut(s) | 637, 705, 719, 1008, 1217, 1291, 1382 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 619, 877, 995, 1016, 1062, 1850 |
| AflIII | ACRYGT | 1 cut(s) | 1426 |
| AjnI | CCWGG | 3 cut(s) | 994, 1090, 1625 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1094, 1126 |
| AluBI | AGCT | 9 cut(s) | 16, 269, 844, 965, 1120, 1136, 1558, 1802, 1885 |
| AluI | AGCT | 9 cut(s) | 16, 269, 844, 965, 1120, 1136, 1558, 1802, 1885 |
| Alw21I | GWGCWC | 2 cut(s) | 795, 1580 |
| Alw26I | GTCTC | 4 cut(s) | 867, 912, 1100, 1536 |
| Alw44I | GTGCAC | 1 cut(s) | 791 |
| AlwI | GGATC | 2 cut(s) | 1623, 1826 |
| Ama87I | CYCGRG | 1 cut(s) | 1170 |
| Aor13HI | TCCGGA | 1 cut(s) | 514 |
| AoxI | GGCC | 2 cut(s) | 436, 723 |
| ApaLI | GTGCAC | 1 cut(s) | 791 |
| ApeKI | GCWGC | 4 cut(s) | 770, 844, 1871, 1920 |
| ApoI | RAATTY | 3 cut(s) | 205, 1207, 1763 |
| AseI | ATTAAT | 1 cut(s) | 1274 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1394 |
| Asp718I | GGTACC | 1 cut(s) | 635 |
| AspA2I | CCTAGG | 1 cut(s) | 1462 |
| AspLEI | GCGC | 2 cut(s) | 649, 923 |
| AspS9I | GGNCC | 7 cut(s) | 105, 437, 742, 986, 1166, 1473, 1675 |
| AsuC2I | CCSGG | 1 cut(s) | 1690 |
| AsuHPI | GGTGA | 3 cut(s) | 65, 224, 1457 |
| AvaI | CYCGRG | 1 cut(s) | 1170 |
| AvaII | GGWCC | 6 cut(s) | 105, 742, 986, 1166, 1473, 1675 |
| AvrII | CCTAGG | 1 cut(s) | 1462 |
| AxyI | CCTNAGG | 1 cut(s) | 526 |
| BaeGI | GKGCMC | 1 cut(s) | 795 |
| BanI | GGYRCC | 2 cut(s) | 635, 646 |
| BanII | GRGCYC | 1 cut(s) | 982 |
| BbsI | GAAGAC | 2 cut(s) | 1666, 1712 |
| Bbv12I | GWGCWC | 2 cut(s) | 795, 1580 |
| BbvCI | CCTCAGC | 1 cut(s) | 1758 |
| BbvI | GCAGC | 4 cut(s) | 757, 831, 1883, 1907 |
| BccI | CCATC | 2 cut(s) | 613, 689 |
| BceAI | ACGGC | 1 cut(s) | 783 |
| BciT130I | CCWGG | 3 cut(s) | 996, 1092, 1627 |
| BciVI | GTATCC | 1 cut(s) | 1140 |
| BclI | TGATCA | 4 cut(s) | 262, 658, 750, 1369 |
| BcnI | CCSGG | 1 cut(s) | 1690 |
| BcoDI | GTCTC | 4 cut(s) | 867, 912, 1100, 1536 |
| BfaI | CTAG | 5 cut(s) | 819, 1199, 1416, 1463, 1940 |
| BfmI | CTRYAG | 1 cut(s) | 771 |
| BfoI | RGCGCY | 1 cut(s) | 650 |
| BfuAI | ACCTGC | 1 cut(s) | 2021 |
| BfuI | GTATCC | 1 cut(s) | 1140 |
| BisI | GCNGC | 4 cut(s) | 771, 845, 1872, 1921 |
| BlnI | CCTAGG | 1 cut(s) | 1462 |
| BlpI | GCTNAGC | 1 cut(s) | 966 |
| BlsI | GCNGC | 4 cut(s) | 772, 846, 1873, 1922 |
| Bme1390I | CCNGG | 4 cut(s) | 996, 1092, 1627, 1690 |
| Bme18I | GGWCC | 6 cut(s) | 105, 742, 986, 1166, 1473, 1675 |
| BmeT110I | CYCGRG | 1 cut(s) | 1170 |
| BmgT120I | GGNCC | 7 cut(s) | 105, 437, 742, 986, 1166, 1473, 1675 |
| BmiI | GGNNCC | 4 cut(s) | 637, 648, 1474, 1551 |
| BmrFI | CCNGG | 4 cut(s) | 996, 1092, 1627, 1690 |
| BmrI | ACTGGG | 1 cut(s) | 135 |
| BmsI | GCATC | 4 cut(s) | 38, 805, 1722, 1924 |
| BmuI | ACTGGG | 1 cut(s) | 135 |
| BpiI | GAAGAC | 2 cut(s) | 1666, 1712 |
| Bpu10I | CCTNAGC | 3 cut(s) | 1155, 1758, 1950 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 966 |
| BpuEI | CTTGAG | 1 cut(s) | 966 |
| BpuMI | CCSGG | 1 cut(s) | 1690 |
| BsaHI | GRCGYC | 1 cut(s) | 647 |
| BsaJI | CCNNGG | 8 cut(s) | 834, 1101, 1219, 1462, 1626, 1689, 1700, 1807 |
| BsaWI | WCCGGW | 1 cut(s) | 514 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1842, 1872 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 619, 877, 995, 1016, 1062, 1850 |
| Bse118I | RCCGGY | 1 cut(s) | 68 |
| Bse1I | ACTGG | 4 cut(s) | 130, 637, 796, 1297 |
| Bse21I | CCTNAGG | 1 cut(s) | 526 |
| BseAI | TCCGGA | 1 cut(s) | 514 |
| BseBI | CCWGG | 3 cut(s) | 996, 1092, 1627 |
| BseDI | CCNNGG | 8 cut(s) | 834, 1101, 1219, 1462, 1626, 1689, 1700, 1807 |
| BseGI | GGATG | 2 cut(s) | 891, 907 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 619, 877, 995, 1016, 1062, 1850 |
| BseMII | CTCAG | 6 cut(s) | 333, 540, 817, 923, 1049, 1749 |
| BseNI | ACTGG | 4 cut(s) | 130, 637, 796, 1297 |
| BseRI | GAGGAG | 1 cut(s) | 555 |
| BseSI | GKGCMC | 1 cut(s) | 795 |
| BseXI | GCAGC | 4 cut(s) | 757, 831, 1883, 1907 |
| BseYI | CCCAGC | 1 cut(s) | 1554 |
| BshFI | GGCC | 2 cut(s) | 438, 725 |
| BshNI | GGYRCC | 2 cut(s) | 635, 646 |
| BsiHKAI | GWGCWC | 2 cut(s) | 795, 1580 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1170 |
| BsiSI | CCGG | 3 cut(s) | 69, 515, 1689 |
| BsiWI | CGTACG | 1 cut(s) | 1380 |
| BslFI | GGGAC | 1 cut(s) | 1011 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 619, 877, 995, 1016, 1062, 1850 |
| BsmAI | GTCTC | 4 cut(s) | 867, 912, 1100, 1536 |
| BsmFI | GGGAC | 1 cut(s) | 1011 |
| BsmI | GAATGC | 1 cut(s) | 1078 |
| BsnI | GGCC | 2 cut(s) | 438, 725 |
| BsoBI | CYCGRG | 1 cut(s) | 1170 |
| Bsp1286I | GDGCHC | 3 cut(s) | 795, 982, 1580 |
| Bsp13I | TCCGGA | 1 cut(s) | 514 |
| Bsp1407I | TGTACA | 1 cut(s) | 717 |
| Bsp143I | GATC | 8 cut(s) | 145, 262, 658, 750, 1237, 1369, 1615, 1831 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 966 |
| Bsp19I | CCATGG | 1 cut(s) | 1807 |
| BspACI | CCGC | 2 cut(s) | 156, 1812 |
| BspANI | GGCC | 2 cut(s) | 438, 725 |
| BspCNI | CTCAG | 6 cut(s) | 334, 539, 816, 922, 1048, 1750 |
| BspEI | TCCGGA | 1 cut(s) | 514 |
| BspLI | GGNNCC | 4 cut(s) | 637, 648, 1474, 1551 |
| BspMAI | CTGCAG | 1 cut(s) | 775 |
| BspMI | ACCTGC | 1 cut(s) | 2021 |
| BspPI | GGATC | 2 cut(s) | 1623, 1826 |
| BspT107I | GGYRCC | 2 cut(s) | 635, 646 |
| BsrFI | RCCGGY | 1 cut(s) | 68 |
| BsrGI | TGTACA | 1 cut(s) | 717 |
| BsrI | ACTGG | 4 cut(s) | 130, 637, 796, 1297 |
| BssAI | RCCGGY | 1 cut(s) | 68 |
| BssECI | CCNNGG | 8 cut(s) | 834, 1101, 1219, 1462, 1626, 1689, 1700, 1807 |
| BssMI | GATC | 8 cut(s) | 145, 262, 658, 750, 1237, 1369, 1615, 1831 |
| BssNI | GRCGYC | 1 cut(s) | 647 |
| BssT1I | CCWWGG | 6 cut(s) | 834, 1101, 1219, 1462, 1700, 1807 |
| Bst2UI | CCWGG | 3 cut(s) | 996, 1092, 1627 |
| Bst4CI | ACNGT | 5 cut(s) | 708, 830, 958, 1006, 1665 |
| Bst6I | CTCTTC | 1 cut(s) | 771 |
| BstACI | GRCGYC | 1 cut(s) | 647 |
| BstAUI | TGTACA | 1 cut(s) | 717 |
| BstC8I | GCNNGC | 4 cut(s) | 52, 436, 1151, 1863 |
| BstDSI | CCRYGG | 1 cut(s) | 1807 |
| BstEII | GGTNACC | 1 cut(s) | 71 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 875 |
| BstF5I | GGATG | 2 cut(s) | 891, 907 |
| BstH2I | RGCGCY | 1 cut(s) | 650 |
| BstHHI | GCGC | 2 cut(s) | 649, 923 |
| BstKTI | GATC | 8 cut(s) | 148, 265, 661, 753, 1240, 1372, 1618, 1834 |
| BstMAI | GTCTC | 4 cut(s) | 867, 912, 1100, 1536 |
| BstMBI | GATC | 8 cut(s) | 145, 262, 658, 750, 1237, 1369, 1615, 1831 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 471, 853, 1871 |
| BstNI | CCWGG | 3 cut(s) | 996, 1092, 1627 |
| BstNSI | RCATGY | 2 cut(s) | 1100, 1430 |
| BstPI | GGTNACC | 1 cut(s) | 71 |
| BstSCI | CCNGG | 4 cut(s) | 994, 1090, 1625, 1688 |
| BstSFI | CTRYAG | 1 cut(s) | 771 |
| BstSLI | GKGCMC | 1 cut(s) | 795 |
| BstV1I | GCAGC | 4 cut(s) | 757, 831, 1883, 1907 |
| BstV2I | GAAGAC | 2 cut(s) | 1666, 1712 |
| BstX2I | RGATCY | 1 cut(s) | 1615 |
| BstYI | RGATCY | 1 cut(s) | 1615 |
| Bsu36I | CCTNAGG | 1 cut(s) | 526 |
| BsuI | GTATCC | 1 cut(s) | 1140 |
| BsuRI | GGCC | 2 cut(s) | 438, 725 |
| BtgI | CCRYGG | 1 cut(s) | 1807 |
| BtsCI | GGATG | 2 cut(s) | 891, 907 |
| BtsIMutI | CAGTG | 4 cut(s) | 630, 811, 835, 1002 |
| BveI | ACCTGC | 1 cut(s) | 2021 |
| Cac8I | GCNNGC | 4 cut(s) | 52, 436, 1151, 1863 |
| CfoI | GCGC | 2 cut(s) | 649, 923 |
| Cfr10I | RCCGGY | 1 cut(s) | 68 |
| Cfr13I | GGNCC | 7 cut(s) | 105, 437, 742, 986, 1166, 1473, 1675 |
| Csp6I | GTAC | 7 cut(s) | 636, 704, 718, 1007, 1216, 1290, 1381 |
| CviQI | GTAC | 7 cut(s) | 636, 704, 718, 1007, 1216, 1290, 1381 |
| DinI | GGCGCC | 1 cut(s) | 648 |
| DpnI | GATC | 8 cut(s) | 147, 264, 660, 752, 1239, 1371, 1617, 1833 |
| DpnII | GATC | 8 cut(s) | 145, 262, 658, 750, 1237, 1369, 1615, 1831 |
| DraI | TTTAAA | 1 cut(s) | 1314 |
| Eam1104I | CTCTTC | 1 cut(s) | 771 |
| EarI | CTCTTC | 1 cut(s) | 771 |
| EciI | GGCGGA | 1 cut(s) | 1827 |
| Eco130I | CCWWGG | 6 cut(s) | 834, 1101, 1219, 1462, 1700, 1807 |
| Eco24I | GRGCYC | 1 cut(s) | 982 |
| Eco47I | GGWCC | 6 cut(s) | 105, 742, 986, 1166, 1473, 1675 |
| Eco57I | CTGAAG | 3 cut(s) | 32, 1251, 1977 |
| Eco81I | CCTNAGG | 1 cut(s) | 526 |
| Eco88I | CYCGRG | 1 cut(s) | 1170 |
| Eco91I | GGTNACC | 1 cut(s) | 71 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 875 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1166 |
| EcoO65I | GGTNACC | 1 cut(s) | 71 |
| EcoRI | GAATTC | 1 cut(s) | 1207 |
| EcoRII | CCWGG | 3 cut(s) | 994, 1090, 1625 |
| EcoT14I | CCWWGG | 6 cut(s) | 834, 1101, 1219, 1462, 1700, 1807 |
| EcoT22I | ATGCAT | 1 cut(s) | 309 |
| EcoT38I | GRGCYC | 1 cut(s) | 982 |
| EgeI | GGCGCC | 1 cut(s) | 648 |
| EheI | GGCGCC | 1 cut(s) | 648 |
| ErhI | CCWWGG | 6 cut(s) | 834, 1101, 1219, 1462, 1700, 1807 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 38, 70, 240, 272, 1874, 1906 |
| FaqI | GGGAC | 1 cut(s) | 1011 |
| FauNDI | CATATG | 2 cut(s) | 1374, 1684 |
| FbaI | TGATCA | 4 cut(s) | 262, 658, 750, 1369 |
| FblI | GTMKAC | 1 cut(s) | 1779 |
| Fnu4HI | GCNGC | 4 cut(s) | 771, 845, 1872, 1921 |
| FokI | GGATG | 2 cut(s) | 878, 914 |
| FriOI | GRGCYC | 1 cut(s) | 982 |
| Fsp4HI | GCNGC | 4 cut(s) | 771, 845, 1872, 1921 |
| FspBI | CTAG | 5 cut(s) | 819, 1199, 1416, 1463, 1940 |
| GlaI | GCGC | 2 cut(s) | 648, 922 |
| GluI | GCNGC | 4 cut(s) | 771, 845, 1872, 1921 |
| GsaI | CCCAGC | 1 cut(s) | 1558 |
| HaeII | RGCGCY | 1 cut(s) | 650 |
| HaeIII | GGCC | 2 cut(s) | 438, 725 |
| HapII | CCGG | 3 cut(s) | 69, 515, 1689 |
| HhaI | GCGC | 2 cut(s) | 649, 923 |
| Hin1I | GRCGYC | 1 cut(s) | 647 |
| Hin6I | GCGC | 2 cut(s) | 647, 921 |
| HinP1I | GCGC | 2 cut(s) | 647, 921 |
| HincII | GTYRAC | 2 cut(s) | 378, 1258 |
| HindII | GTYRAC | 2 cut(s) | 378, 1258 |
| HindIII | AAGCTT | 1 cut(s) | 267 |
| HpaII | CCGG | 3 cut(s) | 69, 515, 1689 |
| HphI | GGTGA | 3 cut(s) | 65, 224, 1457 |
| Hpy166II | GTNNAC | 5 cut(s) | 378, 793, 989, 1258, 1780 |
| Hpy188III | TCNNGA | 5 cut(s) | 368, 515, 1017, 1613, 1673 |
| Hpy8I | GTNNAC | 5 cut(s) | 378, 793, 989, 1258, 1780 |
| HpyAV | CCTTC | 8 cut(s) | 395, 452, 611, 876, 981, 1275, 1462, 1978 |
| HpyCH4III | ACNGT | 5 cut(s) | 708, 830, 958, 1006, 1665 |
| HpyCH4IV | ACGT | 1 cut(s) | 1379 |
| HpyCH4V | TGCA | 9 cut(s) | 307, 405, 773, 793, 1229, 1727, 1871, 1923, 2016 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 471, 853, 1871 |
| HpySE526I | ACGT | 1 cut(s) | 1379 |
| Hsp92I | GRCGYC | 1 cut(s) | 647 |
| HspAI | GCGC | 2 cut(s) | 647, 921 |
| KasI | GGCGCC | 1 cut(s) | 646 |
| Kpn2I | TCCGGA | 1 cut(s) | 514 |
| KpnI | GGTACC | 1 cut(s) | 639 |
| Ksp22I | TGATCA | 4 cut(s) | 262, 658, 750, 1369 |
| Kzo9I | GATC | 8 cut(s) | 145, 262, 658, 750, 1237, 1369, 1615, 1831 |
| LmnI | GCTCC | 3 cut(s) | 129, 542, 784 |
| Lsp1109I | GCAGC | 4 cut(s) | 757, 831, 1883, 1907 |
| LweI | GCATC | 4 cut(s) | 38, 805, 1722, 1924 |
| MaeI | CTAG | 5 cut(s) | 819, 1199, 1416, 1463, 1940 |
| MaeII | ACGT | 1 cut(s) | 1379 |
| MaeIII | GTNAC | 5 cut(s) | 71, 628, 1422, 1483, 1582 |
| MalI | GATC | 8 cut(s) | 147, 264, 660, 752, 1239, 1371, 1617, 1833 |
| MboI | GATC | 8 cut(s) | 145, 262, 658, 750, 1237, 1369, 1615, 1831 |
| MfeI | CAATTG | 2 cut(s) | 138, 746 |
| MflI | RGATCY | 1 cut(s) | 1615 |
| MhlI | GDGCHC | 3 cut(s) | 795, 982, 1580 |
| Mly113I | GGCGCC | 1 cut(s) | 647 |
| MlyI | GAGTC | 5 cut(s) | 869, 914, 1027, 1457, 1491 |
| MmeI | TCCRAC | 1 cut(s) | 762 |
| Mph1103I | ATGCAT | 1 cut(s) | 309 |
| MroI | TCCGGA | 1 cut(s) | 514 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1394 |
| MseI | TTAA | 7 cut(s) | 63, 359, 642, 710, 1274, 1313, 1560 |
| MslI | CAYNNNNRTG | 1 cut(s) | 828 |
| MspA1I | CMGCKG | 1 cut(s) | 1136 |
| MspI | CCGG | 3 cut(s) | 69, 515, 1689 |
| MspR9I | CCNGG | 4 cut(s) | 996, 1092, 1627, 1690 |
| MunI | CAATTG | 2 cut(s) | 138, 746 |
| Mva1269I | GAATGC | 1 cut(s) | 1078 |
| MvaI | CCWGG | 3 cut(s) | 996, 1092, 1627 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 471, 853, 1871 |
| NarI | GGCGCC | 1 cut(s) | 647 |
| NciI | CCSGG | 1 cut(s) | 1690 |
| NcoI | CCATGG | 1 cut(s) | 1807 |
| NdeI | CATATG | 2 cut(s) | 1374, 1684 |
| NdeII | GATC | 8 cut(s) | 145, 262, 658, 750, 1237, 1369, 1615, 1831 |
| NlaIV | GGNNCC | 4 cut(s) | 637, 648, 1474, 1551 |
| NmuCI | GTSAC | 4 cut(s) | 71, 628, 1422, 1483 |
| NsiI | ATGCAT | 1 cut(s) | 309 |
| NspI | RCATGY | 2 cut(s) | 1100, 1430 |
| PaeR7I | CTCGAG | 1 cut(s) | 1170 |
| PciI | ACATGT | 1 cut(s) | 1426 |
| PctI | GAATGC | 1 cut(s) | 1078 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1394 |
| PfeI | GAWTC | 5 cut(s) | 223, 518, 609, 1355, 1712 |
| Pfl23II | CGTACG | 1 cut(s) | 1380 |
| PflFI | GACNNNGTC | 1 cut(s) | 1023 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 619, 995 |
| PkrI | GCNGC | 4 cut(s) | 772, 846, 1873, 1922 |
| PleI | GAGTC | 5 cut(s) | 868, 913, 1027, 1456, 1490 |
| PluTI | GGCGCC | 1 cut(s) | 650 |
| PpsI | GAGTC | 5 cut(s) | 868, 913, 1027, 1456, 1490 |
| PpuMI | RGGWCCY | 1 cut(s) | 1166 |
| PscI | ACATGT | 1 cut(s) | 1426 |
| PshBI | ATTAAT | 1 cut(s) | 1274 |
| PsiI | TTATAA | 1 cut(s) | 1433 |
| Psp5II | RGGWCCY | 1 cut(s) | 1166 |
| Psp6I | CCWGG | 3 cut(s) | 994, 1090, 1625 |
| PspEI | GGTNACC | 1 cut(s) | 71 |
| PspFI | CCCAGC | 1 cut(s) | 1554 |
| PspGI | CCWGG | 3 cut(s) | 994, 1090, 1625 |
| PspLI | CGTACG | 1 cut(s) | 1380 |
| PspN4I | GGNNCC | 4 cut(s) | 637, 648, 1474, 1551 |
| PspPI | GGNCC | 7 cut(s) | 105, 437, 742, 986, 1166, 1473, 1675 |
| PspPPI | RGGWCCY | 1 cut(s) | 1166 |
| PspXI | VCTCGAGB | 1 cut(s) | 1170 |
| PstI | CTGCAG | 1 cut(s) | 775 |
| PsuI | RGATCY | 1 cut(s) | 1615 |
| PsyI | GACNNNGTC | 1 cut(s) | 1023 |
| PvuII | CAGCTG | 1 cut(s) | 1136 |
| RsaI | GTAC | 7 cut(s) | 637, 705, 719, 1008, 1217, 1291, 1382 |
| RsaNI | GTAC | 7 cut(s) | 636, 704, 718, 1007, 1216, 1290, 1381 |
| RseI | CAYNNNNRTG | 1 cut(s) | 828 |
| SaqAI | TTAA | 7 cut(s) | 63, 359, 642, 710, 1274, 1313, 1560 |
| SatI | GCNGC | 4 cut(s) | 771, 845, 1872, 1921 |
| Sau3AI | GATC | 8 cut(s) | 145, 262, 658, 750, 1237, 1369, 1615, 1831 |
| Sau96I | GGNCC | 7 cut(s) | 105, 437, 742, 986, 1166, 1473, 1675 |
| SchI | GAGTC | 5 cut(s) | 869, 914, 1027, 1457, 1491 |
| ScrFI | CCNGG | 4 cut(s) | 996, 1092, 1627, 1690 |
| SduI | GDGCHC | 3 cut(s) | 795, 982, 1580 |
| SfaNI | GCATC | 4 cut(s) | 38, 805, 1722, 1924 |
| SfcI | CTRYAG | 1 cut(s) | 771 |
| SfoI | GGCGCC | 1 cut(s) | 648 |
| Sfr274I | CTCGAG | 1 cut(s) | 1170 |
| SinI | GGWCC | 6 cut(s) | 105, 742, 986, 1166, 1473, 1675 |
| SlaI | CTCGAG | 1 cut(s) | 1170 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 828 |
| SmlI | CTYRAG | 2 cut(s) | 981, 1170 |
| SmoI | CTYRAG | 2 cut(s) | 981, 1170 |
| SsiI | CCGC | 2 cut(s) | 156, 1812 |
| SspDI | GGCGCC | 1 cut(s) | 646 |
| SspI | AATATT | 2 cut(s) | 163, 484 |
| SspMI | CTAG | 5 cut(s) | 819, 1199, 1416, 1463, 1940 |
| StyD4I | CCNGG | 4 cut(s) | 994, 1090, 1625, 1688 |
| StyI | CCWWGG | 6 cut(s) | 834, 1101, 1219, 1462, 1700, 1807 |
| TaaI | ACNGT | 5 cut(s) | 708, 830, 958, 1006, 1665 |
| TaiI | ACGT | 1 cut(s) | 1382 |
| TaqI | TCGA | 3 cut(s) | 355, 369, 1171 |
| TatI | WGTACW | 2 cut(s) | 703, 717 |
| TfiI | GAWTC | 5 cut(s) | 223, 518, 609, 1355, 1712 |
| Tru1I | TTAA | 7 cut(s) | 63, 359, 642, 710, 1274, 1313, 1560 |
| Tru9I | TTAA | 7 cut(s) | 63, 359, 642, 710, 1274, 1313, 1560 |
| TscAI | CASTG | 4 cut(s) | 637, 811, 835, 1009 |
| TseFI | GTSAC | 4 cut(s) | 71, 628, 1422, 1483 |
| TseI | GCWGC | 4 cut(s) | 770, 844, 1871, 1920 |
| Tsp45I | GTSAC | 4 cut(s) | 71, 628, 1422, 1483 |
| TspDTI | ATGAA | 7 cut(s) | 261, 522, 668, 1035, 1387, 1658, 1813 |
| TspGWI | ACGGA | 3 cut(s) | 389, 493, 884 |
| TspRI | CASTG | 4 cut(s) | 637, 811, 835, 1009 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1023 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 619, 995 |
| VneI | GTGCAC | 1 cut(s) | 791 |
| VpaK11BI | GGWCC | 6 cut(s) | 105, 742, 986, 1166, 1473, 1675 |
| VspI | ATTAAT | 1 cut(s) | 1274 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 875 |
| XapI | RAATTY | 3 cut(s) | 205, 1207, 1763 |
| XceI | RCATGY | 2 cut(s) | 1100, 1430 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 803, 1063 |
| XhoI | CTCGAG | 1 cut(s) | 1170 |
| XmaJI | CCTAGG | 1 cut(s) | 1462 |
| XmiI | GTMKAC | 1 cut(s) | 1779 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1394 |
| XspI | CTAG | 5 cut(s) | 819, 1199, 1416, 1463, 1940 |
| Zsp2I | ATGCAT | 1 cut(s) | 309 |