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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 173 |
| AclWI | GGATC | 1 cut(s) | 178 |
| AcoI | YGGCCR | 1 cut(s) | 210 |
| AcuI | CTGAAG | 1 cut(s) | 119 |
| AfaI | GTAC | 1 cut(s) | 38 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 237 |
| AgsI | TTSAA | 2 cut(s) | 89, 110 |
| AluBI | AGCT | 3 cut(s) | 29, 113, 263 |
| AluI | AGCT | 3 cut(s) | 29, 113, 263 |
| Alw26I | GTCTC | 1 cut(s) | 60 |
| AlwI | GGATC | 1 cut(s) | 178 |
| AoxI | GGCC | 1 cut(s) | 210 |
| ApeKI | GCWGC | 2 cut(s) | 139, 242 |
| AsuHPI | GGTGA | 1 cut(s) | 111 |
| AxyI | CCTNAGG | 1 cut(s) | 156 |
| BbvI | GCAGC | 2 cut(s) | 151, 229 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 131, 165 |
| BcoDI | GTCTC | 1 cut(s) | 60 |
| BfaI | CTAG | 3 cut(s) | 15, 96, 101 |
| BfmI | CTRYAG | 2 cut(s) | 249, 258 |
| BisI | GCNGC | 2 cut(s) | 140, 243 |
| BlsI | GCNGC | 2 cut(s) | 141, 244 |
| BsaXI | ACNNNNNCTCC | 1 cut(s) | 25 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 237 |
| Bse21I | CCTNAGG | 1 cut(s) | 156 |
| BseGI | GGATG | 2 cut(s) | 39, 55 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 237 |
| BseMII | CTCAG | 1 cut(s) | 71 |
| BseXI | GCAGC | 2 cut(s) | 151, 229 |
| BshFI | GGCC | 1 cut(s) | 212 |
| BsiSI | CCGG | 1 cut(s) | 213 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 237 |
| BsmAI | GTCTC | 1 cut(s) | 60 |
| BsnI | GGCC | 1 cut(s) | 212 |
| Bsp143I | GATC | 2 cut(s) | 183, 220 |
| BspACI | CCGC | 1 cut(s) | 173 |
| BspANI | GGCC | 1 cut(s) | 212 |
| BspCNI | CTCAG | 1 cut(s) | 70 |
| BspPI | GGATC | 1 cut(s) | 178 |
| BssMI | GATC | 2 cut(s) | 183, 220 |
| BstC8I | GCNNGC | 1 cut(s) | 78 |
| BstDEI | CTNAG | 3 cut(s) | 57, 114, 156 |
| BstF5I | GGATG | 2 cut(s) | 39, 55 |
| BstKTI | GATC | 2 cut(s) | 186, 223 |
| BstMAI | GTCTC | 1 cut(s) | 60 |
| BstMBI | GATC | 2 cut(s) | 183, 220 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 110, 251, 260 |
| BstNSI | RCATGY | 1 cut(s) | 80 |
| BstSFI | CTRYAG | 2 cut(s) | 249, 258 |
| BstV1I | GCAGC | 2 cut(s) | 151, 229 |
| Bsu36I | CCTNAGG | 1 cut(s) | 156 |
| BsuRI | GGCC | 1 cut(s) | 212 |
| BtsCI | GGATG | 2 cut(s) | 39, 55 |
| Cac8I | GCNNGC | 1 cut(s) | 78 |
| Csp6I | GTAC | 1 cut(s) | 37 |
| CviAII | CATG | 3 cut(s) | 77, 269, 321 |
| CviJI | RGCY | 9 cut(s) | 29, 99, 104, 113, 212, 242, 254, 263, 287 |
| CviKI_1 | RGCY | 9 cut(s) | 29, 99, 104, 113, 212, 242, 254, 263, 287 |
| CviQI | GTAC | 1 cut(s) | 37 |
| DdeI | CTNAG | 3 cut(s) | 57, 114, 156 |
| DpnI | GATC | 2 cut(s) | 185, 222 |
| DpnII | GATC | 2 cut(s) | 183, 220 |
| DraI | TTTAAA | 1 cut(s) | 291 |
| EaeI | YGGCCR | 1 cut(s) | 210 |
| Eco57I | CTGAAG | 1 cut(s) | 119 |
| Eco81I | CCTNAGG | 1 cut(s) | 156 |
| FaeI | CATG | 3 cut(s) | 80, 272, 324 |
| FaiI | YATR | 7 cut(s) | 78, 204, 228, 260, 270, 284, 322 |
| FatI | CATG | 3 cut(s) | 76, 268, 320 |
| Fnu4HI | GCNGC | 2 cut(s) | 140, 243 |
| FokI | GGATG | 2 cut(s) | 26, 62 |
| Fsp4HI | GCNGC | 2 cut(s) | 140, 243 |
| FspBI | CTAG | 3 cut(s) | 15, 96, 101 |
| GluI | GCNGC | 2 cut(s) | 140, 243 |
| HaeIII | GGCC | 1 cut(s) | 212 |
| HapII | CCGG | 1 cut(s) | 213 |
| Hin1II | CATG | 3 cut(s) | 80, 272, 324 |
| HinfI | GANTC | 1 cut(s) | 53 |
| HpaII | CCGG | 1 cut(s) | 213 |
| HphI | GGTGA | 1 cut(s) | 111 |
| Hpy188I | TCNGA | 1 cut(s) | 316 |
| HpyAV | CCTTC | 1 cut(s) | 143 |
| HpyCH4IV | ACGT | 1 cut(s) | 35 |
| HpyCH4V | TGCA | 1 cut(s) | 80 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 110, 251, 260 |
| HpyF3I | CTNAG | 3 cut(s) | 57, 114, 156 |
| HpySE526I | ACGT | 1 cut(s) | 35 |
| Hsp92II | CATG | 3 cut(s) | 80, 272, 324 |
| Kzo9I | GATC | 2 cut(s) | 183, 220 |
| LmnI | GCTCC | 1 cut(s) | 250 |
| LpnPI | CCDG | 6 cut(s) | 47, 68, 207, 219, 226, 249 |
| Lsp1109I | GCAGC | 2 cut(s) | 151, 229 |
| MaeI | CTAG | 3 cut(s) | 15, 96, 101 |
| MaeII | ACGT | 1 cut(s) | 35 |
| MaeIII | GTNAC | 1 cut(s) | 117 |
| MalI | GATC | 2 cut(s) | 185, 222 |
| MboI | GATC | 2 cut(s) | 183, 220 |
| MlyI | GAGTC | 1 cut(s) | 62 |
| MnlI | CCTC | 2 cut(s) | 21, 265 |
| MseI | TTAA | 1 cut(s) | 290 |
| MspI | CCGG | 1 cut(s) | 213 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 110, 251, 260 |
| NdeII | GATC | 2 cut(s) | 183, 220 |
| NlaIII | CATG | 3 cut(s) | 80, 272, 324 |
| NmuCI | GTSAC | 1 cut(s) | 117 |
| NspI | RCATGY | 1 cut(s) | 80 |
| PaeI | GCATGC | 1 cut(s) | 80 |
| PkrI | GCNGC | 2 cut(s) | 141, 244 |
| PleI | GAGTC | 1 cut(s) | 61 |
| PpsI | GAGTC | 1 cut(s) | 61 |
| RsaI | GTAC | 1 cut(s) | 38 |
| RsaNI | GTAC | 1 cut(s) | 37 |
| SaqAI | TTAA | 1 cut(s) | 290 |
| SatI | GCNGC | 2 cut(s) | 140, 243 |
| Sau3AI | GATC | 2 cut(s) | 183, 220 |
| SchI | GAGTC | 1 cut(s) | 62 |
| SetI | ASST | 8 cut(s) | 31, 38, 87, 115, 130, 238, 265, 297 |
| SfcI | CTRYAG | 2 cut(s) | 249, 258 |
| SphI | GCATGC | 1 cut(s) | 80 |
| SsiI | CCGC | 1 cut(s) | 173 |
| SspMI | CTAG | 3 cut(s) | 15, 96, 101 |
| TaiI | ACGT | 1 cut(s) | 38 |
| Tru1I | TTAA | 1 cut(s) | 290 |
| Tru9I | TTAA | 1 cut(s) | 290 |
| TseFI | GTSAC | 1 cut(s) | 117 |
| TseI | GCWGC | 2 cut(s) | 139, 242 |
| Tsp45I | GTSAC | 1 cut(s) | 117 |
| TspGWI | ACGGA | 1 cut(s) | 32 |
| XceI | RCATGY | 1 cut(s) | 80 |
| XspI | CTAG | 3 cut(s) | 15, 96, 101 |