Rroxscaffold_3G00270860
RLK Family

Cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000146
Physical Location & Seq
Forward (+)
63329138 .. 63334331
5194 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00270860.1

Sequence Viewer

Length: 480 bp
ATGTATGTTATCTTTCGGAATATGTTCAATCGAGTGATCATTGGCAAGGGAGACCGAGGGGGAGACTGTTTCACTTGGATTACATGTATGTTGGACAAACACCGGGACAACCTTTGGCCCGGCATTGAGTTCGATCGGCTAAGGGAGTTATGGTTATGGCACGGACGTTTGGGTCATCCATCTTTTAGAGTTATGAAGAAGTCCATGCCTTCGTTATTTTTGGGAATAAGTGACTCTAGCTTGCATTGTGAAACTTGTACTTTAGCTAAGAGTCATAAGTCTAGTTATCCTTCGAGCTTTCATTCTAGTACTACGCCTTTTGAGTTAATTCATTCGGACTTGTGGGGTCCTTCTAAACATTCTACCCTTTCGGAATGCGATATTTTGTGTTATTCATCGATGACTTTACTCGACTTGTCTTGGGTAGTTTTACTTAAGTCCAAAGATTTAGTTTTCTCTCGCCTTTACAGCATTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Retrotran_gag_2

Protein Analysis

159

Amino Acids

18.37

Weight (kDa)

8.56

Isoelectric Point (pI)

48.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
gag_pre-integrs PF13976 48 - 91 2.8e-12 GAG-pre-integrase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000146)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19561 FvH4_1g25371 FvH4_1g25961 FvH4_2g00352 FvH4_2g00861 FvH4_2g04952 FvH4_2g05413 FvH4_2g07531 FvH4_2g08961 FvH4_2g25141 FvH4_2g27292 FvH4_3g18164 FvH4_3g19921 FvH4_3g43870 FvH4_4g14441 FvH4_4g15581 FvH4_4g15582 FvH4_4g16890 FvH4_4g19782 FvH4_4g33444 FvH4_4g33921 FvH4_5g22281 FvH4_5g22282 FvH4_5g32571 FvH4_5g37451 FvH4_5g39631 FvH4_6g04721 FvH4_6g11661 FvH4_6g20571 FvH4_6g32791 FvH4_7g23184
prunus_persica Prupe.1G090900_v2.0.a1 Prupe.1G241200_v2.0.a1 Prupe.1G534100_v2.0.a1 Prupe.3G076700_v2.0.a1 Prupe.4G150800_v2.0.a1 Prupe.4G181800_v2.0.a1 Prupe.4G240900_v2.0.a1 Prupe.4G247600_v2.0.a1 Prupe.6G115900_v2.0.a1 Prupe.6G327500_v2.0.a1 Prupe.8G071700_v2.0.a1
pyrus_communis pycom06g11480 pycom10g08330 pycom15g04320 pycom15g04350 pycom15g12620 pycom2874g00060
rosa_chinensis RchiOBHm_Chr1g0317241 RchiOBHm_Chr1g0341801 RchiOBHm_Chr1g0341931 RchiOBHm_Chr1g0359621 RchiOBHm_Chr2g0113741 RchiOBHm_Chr2g0117261 RchiOBHm_Chr2g0138831 RchiOBHm_Chr2g0141421 RchiOBHm_Chr2g0145281 RchiOBHm_Chr2g0152991 RchiOBHm_Chr3g0448001 RchiOBHm_Chr3g0450981 RchiOBHm_Chr3g0455161 RchiOBHm_Chr3g0457401 RchiOBHm_Chr3g0460001 RchiOBHm_Chr4g0387431 RchiOBHm_Chr4g0388741 RchiOBHm_Chr4g0444201 RchiOBHm_Chr4g0444261 RchiOBHm_Chr5g0010221 RchiOBHm_Chr6g0271721 RchiOBHm_Chr6g0274641 RchiOBHm_Chr6g0294691 RchiOBHm_Chr7g0237031 RchiOBHm_Chr7g0238461 RchiOBHm_Chr7g0239981
rosa_laevigata RLG00000009017
rosa_multiflora Rmu_co7986768.1_g000001 Rmu_co8302239.1_g000001 Rmu_co8485799.1_g000001 Rmu_sc0000061.1_g000013 Rmu_sc0000303.1_g000034 Rmu_sc0000303.1_g000035 Rmu_sc0000315.1_g000017 Rmu_sc0000349.1_g000003 Rmu_sc0000498.1_g000068 Rmu_sc0000508.1_g000014 Rmu_sc0000602.1_g000001 Rmu_sc0000633.1_g000002 Rmu_sc0000638.1_g000030 Rmu_sc0000756.1_g000016 Rmu_sc0000756.1_g000017 Rmu_sc0000785.1_g000037 Rmu_sc0000795.1_g000017 Rmu_sc0000796.1_g000030 Rmu_sc0000863.1_g000023 Rmu_sc0000920.1_g000021 Rmu_sc0000960.1_g000015 Rmu_sc0000982.1_g000102 Rmu_sc0001036.1_g000008 Rmu_sc0001036.1_g000009 Rmu_sc0001643.1_g000029 Rmu_sc0001687.1_g000026 Rmu_sc0001708.1_g000037 Rmu_sc0001768.1_g000015 Rmu_sc0001790.1_g000022 Rmu_sc0002180.1_g000007 Rmu_sc0002211.1_g000006 Rmu_sc0002270.1_g000010 Rmu_sc0002404.1_g000031 Rmu_sc0002858.1_g000016 Rmu_sc0003063.1_g000028 Rmu_sc0003730.1_g000017 Rmu_sc0004087.1_g000020 Rmu_sc0004618.1_g000048 Rmu_sc0004724.1_g000005 Rmu_sc0005167.1_g000036 Rmu_sc0005781.1_g000003 Rmu_sc0005878.1_g000012 Rmu_sc0006011.1_g000003 Rmu_sc0006343.1_g000011 Rmu_sc0006573.1_g000007 Rmu_sc0006599.1_g000007 Rmu_sc0006803.1_g000014 Rmu_sc0006836.1_g000002 Rmu_sc0006849.1_g000002 Rmu_sc0007109.1_g000009 Rmu_sc0009268.1_g000016 Rmu_sc0009618.1_g000017 Rmu_sc0009674.1_g000009 Rmu_sc0010244.1_g000009 Rmu_sc0011151.1_g000004 Rmu_sc0011356.1_g000001 Rmu_sc0011674.1_g000002 Rmu_sc0011778.1_g000016 Rmu_sc0012342.1_g000001 Rmu_sc0012467.1_g000002 Rmu_sc0012574.1_g000003 Rmu_sc0012913.1_g000005 Rmu_sc0013612.1_g000020 Rmu_sc0014824.1_g000001 Rmu_sc0017869.1_g000002 Rmu_sc0018085.1_g000003 Rmu_sc0024789.1_g000001 Rmu_sc0025106.1_g000013 Rmu_sc0034502.1_g000001 Rmu_sc0034547.1_g000001 Rmu_sc0035094.1_g000001 Rmu_sc0035095.1_g000001 Rmu_ssc0000100.1_g000027 Rmu_ssc0000244.1_g000005 Rmu_ssc0000371.1_g000029 Rmu_ssc0000381.1_g000004
rosa_roxburghii Rroxscaffold_1G00015480 Rroxscaffold_1G00023580 Rroxscaffold_1G00039340 Rroxscaffold_1G00047080 Rroxscaffold_2G00095820 Rroxscaffold_2G00125200 Rroxscaffold_2G00128330 Rroxscaffold_3G00270860
rosa_rugosa Rorug06G0120700
rosa_samantha Rh3BG173100 Rh6AG089600 Rh7AG488100 Rh7DG389600
rosa_wichuraiana Rw0G001150 Rw0G001990 Rw0G002010 Rw0G019540 Rw1G013730 Rw1G024110 Rw1G029190 Rw2G018850 Rw2G021090 Rw2G022260 Rw2G023180 Rw2G027280 Rw3G010190 Rw3G020660 Rw3G029380 Rw4G002390 Rw4G002690 Rw4G033260 Rw5G011960 Rw5G023110 Rw5G025250 Rw5G028950 Rw5G033740 Rw5G033950 Rw5G037900 Rw6G009640 Rw6G011620 Rw6G027720 Rw7G010680 Rw7G042070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 171
AfaI GTAC 2 cut(s) 259, 310
AflII CTTAAG 1 cut(s) 434
AflIII ACRYGT 1 cut(s) 83
AgsI TTSAA 1 cut(s) 28
AluBI AGCT 3 cut(s) 240, 266, 297
AluI AGCT 3 cut(s) 240, 266, 297
Alw26I GTCTC 2 cut(s) 45, 57
AoxI GGCC 1 cut(s) 116
Asp700I GAANNNNTTC 1 cut(s) 23
AspS9I GGNCC 2 cut(s) 117, 347
AsuC2I CCSGG 2 cut(s) 104, 120
AvaII GGWCC 1 cut(s) 347
BccI CCATC 1 cut(s) 187
BcgI CGANNNNNNTGC 2 cut(s) 112, 146
BclI TGATCA 1 cut(s) 36
BcnI CCSGG 2 cut(s) 104, 120
BcoDI GTCTC 2 cut(s) 45, 57
BfaI CTAG 3 cut(s) 237, 282, 306
BfrI CTTAAG 1 cut(s) 434
BmcAI AGTACT 1 cut(s) 310
Bme1390I CCNGG 2 cut(s) 104, 120
Bme18I GGWCC 1 cut(s) 347
BmgT120I GGNCC 2 cut(s) 117, 347
BmiI GGNNCC 1 cut(s) 348
BmrFI CCNGG 2 cut(s) 104, 120
Bpu10I CCTNAGC 1 cut(s) 140
BpuMI CCSGG 2 cut(s) 104, 120
Bsa29I ATCGAT 1 cut(s) 398
BsaI GGTCTC 1 cut(s) 45
BsaJI CCNNGG 1 cut(s) 55
BsaXI ACNNNNNCTCC 2 cut(s) 137, 167
BseCI ATCGAT 1 cut(s) 398
BseDI CCNNGG 1 cut(s) 55
BseGI GGATG 1 cut(s) 175
Bsh1285I CGRYCG 1 cut(s) 136
BshFI GGCC 1 cut(s) 118
BshVI ATCGAT 1 cut(s) 398
BsiEI CGRYCG 1 cut(s) 136
BsiSI CCGG 2 cut(s) 103, 120
BslFI GGGAC 1 cut(s) 119
BsmAI GTCTC 2 cut(s) 45, 57
BsmFI GGGAC 1 cut(s) 119
BsmI GAATGC 1 cut(s) 380
BsnI GGCC 1 cut(s) 118
Bso31I GGTCTC 1 cut(s) 45
Bsp143I GATC 2 cut(s) 36, 133
BspANI GGCC 1 cut(s) 118
BspDI ATCGAT 1 cut(s) 398
BspLI GGNNCC 1 cut(s) 348
BspTI CTTAAG 1 cut(s) 434
BspTNI GGTCTC 1 cut(s) 45
BssECI CCNNGG 1 cut(s) 55
BssMI GATC 2 cut(s) 36, 133
Bst4CI ACNGT 1 cut(s) 68
BstAFI CTTAAG 1 cut(s) 434
BstC8I GCNNGC 1 cut(s) 242
BstDEI CTNAG 2 cut(s) 140, 267
BstF5I GGATG 1 cut(s) 175
BstKTI GATC 2 cut(s) 39, 136
BstMAI GTCTC 2 cut(s) 45, 57
BstMBI GATC 2 cut(s) 36, 133
BstMCI CGRYCG 1 cut(s) 136
BstMWI GCNNNNNNNGC 1 cut(s) 468
BstNSI RCATGY 1 cut(s) 87
BstSCI CCNGG 2 cut(s) 102, 118
Bsu15I ATCGAT 1 cut(s) 398
BsuRI GGCC 1 cut(s) 118
BsuTUI ATCGAT 1 cut(s) 398
BtsCI GGATG 1 cut(s) 175
Cac8I GCNNGC 1 cut(s) 242
Cfr13I GGNCC 2 cut(s) 117, 347
ClaI ATCGAT 1 cut(s) 398
Csp6I GTAC 2 cut(s) 258, 309
CviAII CATG 2 cut(s) 84, 205
CviJI RGCY 5 cut(s) 118, 139, 240, 266, 297
CviKI_1 RGCY 5 cut(s) 118, 139, 240, 266, 297
CviQI GTAC 2 cut(s) 258, 309
DdeI CTNAG 2 cut(s) 140, 267
DpnI GATC 2 cut(s) 38, 135
DpnII GATC 2 cut(s) 36, 133
DrdI GACNNNNNNGTC 1 cut(s) 171
DseDI GACNNNNNNGTC 1 cut(s) 171
Eco31I GGTCTC 1 cut(s) 45
Eco47I GGWCC 1 cut(s) 347
EcoO109I RGGNCCY 1 cut(s) 347
FaeI CATG 2 cut(s) 87, 208
FaqI GGGAC 1 cut(s) 119
FatI CATG 2 cut(s) 83, 204
FbaI TGATCA 1 cut(s) 36
FokI GGATG 1 cut(s) 162
FspBI CTAG 3 cut(s) 237, 282, 306
HaeIII GGCC 1 cut(s) 118
HapII CCGG 2 cut(s) 103, 120
Hin1II CATG 2 cut(s) 87, 208
HinfI GANTC 2 cut(s) 233, 271
HpaII CCGG 2 cut(s) 103, 120
Hpy188I TCNGA 3 cut(s) 18, 337, 373
HpyAV CCTTC 3 cut(s) 219, 300, 360
HpyCH4III ACNGT 1 cut(s) 68
HpyCH4IV ACGT 1 cut(s) 166
HpyCH4V TGCA 1 cut(s) 244
HpyF10VI GCNNNNNNNGC 1 cut(s) 468
HpyF3I CTNAG 2 cut(s) 140, 267
HpySE526I ACGT 1 cut(s) 166
Hsp92II CATG 2 cut(s) 87, 208
Ksp22I TGATCA 1 cut(s) 36
Kzo9I GATC 2 cut(s) 36, 133
LpnPI CCDG 2 cut(s) 116, 133
MaeI CTAG 3 cut(s) 237, 282, 306
MaeII ACGT 1 cut(s) 166
MaeIII GTNAC 1 cut(s) 230
MalI GATC 2 cut(s) 38, 135
MboI GATC 2 cut(s) 36, 133
MboII GAAGA 1 cut(s) 208
MluCI AATT 1 cut(s) 327
MlyI GAGTC 2 cut(s) 227, 280
MmeI TCCRAC 1 cut(s) 72
MnlI CCTC 1 cut(s) 50
MroXI GAANNNNTTC 1 cut(s) 23
MseI TTAA 2 cut(s) 326, 435
MspCI CTTAAG 1 cut(s) 434
MspI CCGG 2 cut(s) 103, 120
MspR9I CCNGG 2 cut(s) 104, 120
Mva1269I GAATGC 1 cut(s) 380
MwoI GCNNNNNNNGC 1 cut(s) 468
NciI CCSGG 2 cut(s) 104, 120
NdeII GATC 2 cut(s) 36, 133
NlaIII CATG 2 cut(s) 87, 208
NlaIV GGNNCC 1 cut(s) 348
NmuCI GTSAC 1 cut(s) 230
NspI RCATGY 1 cut(s) 87
PciI ACATGT 1 cut(s) 83
PctI GAATGC 1 cut(s) 380
PdmI GAANNNNTTC 1 cut(s) 23
Ple19I CGATCG 1 cut(s) 136
PleI GAGTC 2 cut(s) 227, 279
PpsI GAGTC 2 cut(s) 227, 279
PpuMI RGGWCCY 1 cut(s) 347
PscI ACATGT 1 cut(s) 83
Psp5II RGGWCCY 1 cut(s) 347
PspN4I GGNNCC 1 cut(s) 348
PspPI GGNCC 2 cut(s) 117, 347
PspPPI RGGWCCY 1 cut(s) 347
PvuI CGATCG 1 cut(s) 136
RsaI GTAC 2 cut(s) 259, 310
RsaNI GTAC 2 cut(s) 258, 309
SaqAI TTAA 2 cut(s) 326, 435
Sau3AI GATC 2 cut(s) 36, 133
Sau96I GGNCC 2 cut(s) 117, 347
ScaI AGTACT 1 cut(s) 310
SchI GAGTC 2 cut(s) 227, 280
ScrFI CCNGG 2 cut(s) 104, 120
SetI ASST 5 cut(s) 114, 169, 242, 268, 299
SinI GGWCC 1 cut(s) 347
SmlI CTYRAG 1 cut(s) 434
SmoI CTYRAG 1 cut(s) 434
Sse9I AATT 1 cut(s) 327
SspMI CTAG 3 cut(s) 237, 282, 306
StyD4I CCNGG 2 cut(s) 102, 118
TaaI ACNGT 1 cut(s) 68
TaiI ACGT 1 cut(s) 169
TaqI TCGA 5 cut(s) 31, 132, 293, 398, 411
TaqII GACCGA 1 cut(s) 69
TasI AATT 1 cut(s) 327
TatI WGTACW 2 cut(s) 257, 308
Tru1I TTAA 2 cut(s) 326, 435
Tru9I TTAA 2 cut(s) 326, 435
TseFI GTSAC 1 cut(s) 230
Tsp45I GTSAC 1 cut(s) 230
TspDTI ATGAA 5 cut(s) 209, 290, 320, 384, 465
TspGWI ACGGA 1 cut(s) 177
Vha464I CTTAAG 1 cut(s) 434
VpaK11BI GGWCC 1 cut(s) 347
XceI RCATGY 1 cut(s) 87
XmnI GAANNNNTTC 1 cut(s) 23
XspI CTAG 3 cut(s) 237, 282, 306
ZrmI AGTACT 1 cut(s) 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.