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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AgsI | TTSAA | 1 cut(s) | 54 |
| AspS9I | GGNCC | 1 cut(s) | 115 |
| AvaII | GGWCC | 1 cut(s) | 115 |
| Bme18I | GGWCC | 1 cut(s) | 115 |
| BmgT120I | GGNCC | 1 cut(s) | 115 |
| BsaJI | CCNNGG | 1 cut(s) | 132 |
| BseDI | CCNNGG | 1 cut(s) | 132 |
| BseGI | GGATG | 1 cut(s) | 128 |
| BssECI | CCNNGG | 1 cut(s) | 132 |
| BstDSI | CCRYGG | 1 cut(s) | 132 |
| BstF5I | GGATG | 1 cut(s) | 128 |
| BtgI | CCRYGG | 1 cut(s) | 132 |
| BtsCI | GGATG | 1 cut(s) | 128 |
| Cfr13I | GGNCC | 1 cut(s) | 115 |
| CviAII | CATG | 1 cut(s) | 138 |
| Eco47I | GGWCC | 1 cut(s) | 115 |
| FaeI | CATG | 1 cut(s) | 141 |
| FaiI | YATR | 1 cut(s) | 139 |
| FatI | CATG | 1 cut(s) | 137 |
| FokI | GGATG | 1 cut(s) | 115 |
| Hin1II | CATG | 1 cut(s) | 141 |
| Hpy188III | TCNNGA | 1 cut(s) | 118 |
| Hsp92II | CATG | 1 cut(s) | 141 |
| LpnPI | CCDG | 2 cut(s) | 52, 131 |
| MluCI | AATT | 3 cut(s) | 49, 74, 101 |
| NlaIII | CATG | 1 cut(s) | 141 |
| PspPI | GGNCC | 1 cut(s) | 115 |
| Sau96I | GGNCC | 1 cut(s) | 115 |
| SgeI | CNNG | 3 cut(s) | 23, 51, 130 |
| SinI | GGWCC | 1 cut(s) | 115 |
| Sse9I | AATT | 3 cut(s) | 49, 74, 101 |
| TaqII | GACCGA | 1 cut(s) | 103 |
| TasI | AATT | 3 cut(s) | 49, 74, 101 |
| VpaK11BI | GGWCC | 1 cut(s) | 115 |