Peptidyl-prolyl cis-trans isomerase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 218 |
| AatII | GACGTC | 1 cut(s) | 753 |
| Acc36I | ACCTGC | 1 cut(s) | 1199 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 901 |
| AccII | CGCG | 4 cut(s) | 807, 822, 824, 834 |
| AciI | CCGC | 2 cut(s) | 116, 820 |
| AclI | AACGTT | 1 cut(s) | 978 |
| AclWI | GGATC | 1 cut(s) | 248 |
| AcsI | RAATTY | 3 cut(s) | 461, 946, 1019 |
| AcvI | CACGTG | 1 cut(s) | 995 |
| AcyI | GRCGYC | 4 cut(s) | 700, 750, 761, 772 |
| AfaI | GTAC | 4 cut(s) | 525, 540, 1213, 1249 |
| AfiI | CCNNNNNNNGG | 9 cut(s) | 648, 649, 680, 693, 714, 741, 825, 826, 901 |
| AflIII | ACRYGT | 1 cut(s) | 992 |
| AhlI | ACTAGT | 1 cut(s) | 1202 |
| AjnI | CCWGG | 1 cut(s) | 763 |
| AluBI | AGCT | 2 cut(s) | 66, 1254 |
| AluI | AGCT | 2 cut(s) | 66, 1254 |
| Alw21I | GWGCWC | 1 cut(s) | 813 |
| Alw26I | GTCTC | 1 cut(s) | 1167 |
| Alw44I | GTGCAC | 1 cut(s) | 809 |
| AlwI | GGATC | 1 cut(s) | 248 |
| Ama87I | CYCGRG | 3 cut(s) | 659, 727, 735 |
| AoxI | GGCC | 3 cut(s) | 113, 683, 691 |
| ApaI | GGGCCC | 1 cut(s) | 687 |
| ApaLI | GTGCAC | 1 cut(s) | 809 |
| ApoI | RAATTY | 3 cut(s) | 461, 946, 1019 |
| AspA2I | CCTAGG | 1 cut(s) | 509 |
| AspLEI | GCGC | 4 cut(s) | 780, 807, 824, 834 |
| AspS9I | GGNCC | 4 cut(s) | 683, 684, 691, 904 |
| AsuHPI | GGTGA | 1 cut(s) | 158 |
| AvaI | CYCGRG | 3 cut(s) | 659, 727, 735 |
| AvaII | GGWCC | 1 cut(s) | 904 |
| AvrII | CCTAGG | 1 cut(s) | 509 |
| BaeGI | GKGCMC | 2 cut(s) | 687, 813 |
| BanII | GRGCYC | 2 cut(s) | 687, 736 |
| BbrPI | CACGTG | 1 cut(s) | 995 |
| Bbv12I | GWGCWC | 1 cut(s) | 813 |
| BccI | CCATC | 1 cut(s) | 31 |
| BciT130I | CCWGG | 1 cut(s) | 765 |
| BcoDI | GTCTC | 1 cut(s) | 1167 |
| BcuI | ACTAGT | 1 cut(s) | 1202 |
| BfaI | CTAG | 5 cut(s) | 63, 228, 510, 1203, 1251 |
| BfmI | CTRYAG | 1 cut(s) | 67 |
| BfuAI | ACCTGC | 1 cut(s) | 1199 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 769 |
| BisI | GCNGC | 1 cut(s) | 116 |
| BlnI | CCTAGG | 1 cut(s) | 509 |
| BlsI | GCNGC | 1 cut(s) | 117 |
| Bme1390I | CCNGG | 1 cut(s) | 765 |
| Bme18I | GGWCC | 1 cut(s) | 904 |
| BmeT110I | CYCGRG | 3 cut(s) | 659, 727, 735 |
| BmgT120I | GGNCC | 4 cut(s) | 683, 684, 691, 904 |
| BmiI | GGNNCC | 2 cut(s) | 672, 685 |
| BmrFI | CCNGG | 1 cut(s) | 765 |
| BmsI | GCATC | 1 cut(s) | 1284 |
| BpmI | CTGGAG | 2 cut(s) | 691, 1027 |
| BpuEI | CTTGAG | 1 cut(s) | 1358 |
| Bsa29I | ATCGAT | 1 cut(s) | 1370 |
| BsaAI | YACGTR | 1 cut(s) | 995 |
| BsaHI | GRCGYC | 4 cut(s) | 700, 750, 761, 772 |
| BsaJI | CCNNGG | 3 cut(s) | 509, 687, 764 |
| Bsc4I | CCNNNNNNNGG | 9 cut(s) | 648, 649, 680, 693, 714, 741, 825, 826, 901 |
| Bse118I | RCCGGY | 1 cut(s) | 693 |
| Bse1I | ACTGG | 1 cut(s) | 1377 |
| BseBI | CCWGG | 1 cut(s) | 765 |
| BseCI | ATCGAT | 1 cut(s) | 1370 |
| BseDI | CCNNGG | 3 cut(s) | 509, 687, 764 |
| BseGI | GGATG | 1 cut(s) | 23 |
| BseLI | CCNNNNNNNGG | 9 cut(s) | 648, 649, 680, 693, 714, 741, 825, 826, 901 |
| BseMII | CTCAG | 1 cut(s) | 960 |
| BseNI | ACTGG | 1 cut(s) | 1377 |
| BseSI | GKGCMC | 2 cut(s) | 687, 813 |
| BseYI | CCCAGC | 1 cut(s) | 1254 |
| BsgI | GTGCAG | 1 cut(s) | 1227 |
| Bsh1236I | CGCG | 4 cut(s) | 807, 822, 824, 834 |
| BshFI | GGCC | 3 cut(s) | 115, 685, 693 |
| BshVI | ATCGAT | 1 cut(s) | 1370 |
| BsiHKAI | GWGCWC | 1 cut(s) | 813 |
| BsiHKCI | CYCGRG | 3 cut(s) | 659, 727, 735 |
| BsiSI | CCGG | 1 cut(s) | 694 |
| BslFI | GGGAC | 1 cut(s) | 678 |
| BslI | CCNNNNNNNGG | 9 cut(s) | 648, 649, 680, 693, 714, 741, 825, 826, 901 |
| BsmAI | GTCTC | 1 cut(s) | 1167 |
| BsmFI | GGGAC | 1 cut(s) | 678 |
| BsmI | GAATGC | 1 cut(s) | 637 |
| BsnI | GGCC | 3 cut(s) | 115, 685, 693 |
| BsoBI | CYCGRG | 3 cut(s) | 659, 727, 735 |
| Bsp120I | GGGCCC | 1 cut(s) | 683 |
| Bsp1286I | GDGCHC | 3 cut(s) | 687, 736, 813 |
| Bsp143I | GATC | 5 cut(s) | 240, 414, 912, 965, 972 |
| BspACI | CCGC | 2 cut(s) | 116, 820 |
| BspANI | GGCC | 3 cut(s) | 115, 685, 693 |
| BspCNI | CTCAG | 1 cut(s) | 961 |
| BspDI | ATCGAT | 1 cut(s) | 1370 |
| BspFNI | CGCG | 4 cut(s) | 807, 822, 824, 834 |
| BspLI | GGNNCC | 2 cut(s) | 672, 685 |
| BspMI | ACCTGC | 1 cut(s) | 1199 |
| BspPI | GGATC | 1 cut(s) | 248 |
| BsrFI | RCCGGY | 1 cut(s) | 693 |
| BsrI | ACTGG | 1 cut(s) | 1377 |
| BssAI | RCCGGY | 1 cut(s) | 693 |
| BssECI | CCNNGG | 3 cut(s) | 509, 687, 764 |
| BssMI | GATC | 5 cut(s) | 240, 414, 912, 965, 972 |
| BssNI | GRCGYC | 4 cut(s) | 700, 750, 761, 772 |
| BssT1I | CCWWGG | 2 cut(s) | 509, 687 |
| Bst2UI | CCWGG | 1 cut(s) | 765 |
| Bst4CI | ACNGT | 9 cut(s) | 142, 441, 562, 647, 885, 908, 922, 961, 985 |
| BstACI | GRCGYC | 4 cut(s) | 700, 750, 761, 772 |
| BstBAI | YACGTR | 1 cut(s) | 995 |
| BstC8I | GCNNGC | 2 cut(s) | 724, 809 |
| BstDEI | CTNAG | 4 cut(s) | 29, 969, 1386, 1443 |
| BstF5I | GGATG | 1 cut(s) | 23 |
| BstFNI | CGCG | 4 cut(s) | 807, 822, 824, 834 |
| BstHHI | GCGC | 4 cut(s) | 780, 807, 824, 834 |
| BstKTI | GATC | 5 cut(s) | 243, 417, 915, 968, 975 |
| BstMAI | GTCTC | 1 cut(s) | 1167 |
| BstMBI | GATC | 5 cut(s) | 240, 414, 912, 965, 972 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 769 |
| BstNI | CCWGG | 1 cut(s) | 765 |
| BstSCI | CCNGG | 1 cut(s) | 763 |
| BstSFI | CTRYAG | 1 cut(s) | 67 |
| BstSLI | GKGCMC | 2 cut(s) | 687, 813 |
| BstUI | CGCG | 4 cut(s) | 807, 822, 824, 834 |
| Bsu15I | ATCGAT | 1 cut(s) | 1370 |
| BsuRI | GGCC | 3 cut(s) | 115, 685, 693 |
| BsuTUI | ATCGAT | 1 cut(s) | 1370 |
| BtsCI | GGATG | 1 cut(s) | 23 |
| BveI | ACCTGC | 1 cut(s) | 1199 |
| Cac8I | GCNNGC | 2 cut(s) | 724, 809 |
| CfoI | GCGC | 4 cut(s) | 780, 807, 824, 834 |
| Cfr10I | RCCGGY | 1 cut(s) | 693 |
| Cfr13I | GGNCC | 4 cut(s) | 683, 684, 691, 904 |
| ClaI | ATCGAT | 1 cut(s) | 1370 |
| CseI | GACGC | 4 cut(s) | 689, 761, 769, 823 |
| Csp6I | GTAC | 4 cut(s) | 524, 539, 1212, 1248 |
| CviAII | CATG | 3 cut(s) | 18, 385, 896 |
| CviQI | GTAC | 4 cut(s) | 524, 539, 1212, 1248 |
| DdeI | CTNAG | 4 cut(s) | 29, 969, 1386, 1443 |
| DpnI | GATC | 5 cut(s) | 242, 416, 914, 967, 974 |
| DpnII | GATC | 5 cut(s) | 240, 414, 912, 965, 972 |
| DraI | TTTAAA | 2 cut(s) | 322, 330 |
| Eco130I | CCWWGG | 2 cut(s) | 509, 687 |
| Eco24I | GRGCYC | 2 cut(s) | 687, 736 |
| Eco32I | GATATC | 1 cut(s) | 60 |
| Eco47I | GGWCC | 1 cut(s) | 904 |
| Eco72I | CACGTG | 1 cut(s) | 995 |
| Eco88I | CYCGRG | 3 cut(s) | 659, 727, 735 |
| EcoO109I | RGGNCCY | 1 cut(s) | 683 |
| EcoRII | CCWGG | 1 cut(s) | 763 |
| EcoRV | GATATC | 1 cut(s) | 60 |
| EcoT14I | CCWWGG | 2 cut(s) | 509, 687 |
| EcoT38I | GRGCYC | 2 cut(s) | 687, 736 |
| ErhI | CCWWGG | 2 cut(s) | 509, 687 |
| FaeI | CATG | 3 cut(s) | 21, 388, 899 |
| FaqI | GGGAC | 1 cut(s) | 678 |
| FatI | CATG | 3 cut(s) | 17, 384, 895 |
| FauI | CCCGC | 1 cut(s) | 827 |
| Fnu4HI | GCNGC | 1 cut(s) | 116 |
| FokI | GGATG | 1 cut(s) | 10 |
| FriOI | GRGCYC | 2 cut(s) | 687, 736 |
| Fsp4HI | GCNGC | 1 cut(s) | 116 |
| FspBI | CTAG | 5 cut(s) | 63, 228, 510, 1203, 1251 |
| GlaI | GCGC | 4 cut(s) | 779, 806, 823, 833 |
| GluI | GCNGC | 1 cut(s) | 116 |
| GsaI | CCCAGC | 1 cut(s) | 1258 |
| GsuI | CTGGAG | 2 cut(s) | 691, 1027 |
| HaeIII | GGCC | 3 cut(s) | 115, 685, 693 |
| HapII | CCGG | 1 cut(s) | 694 |
| HgaI | GACGC | 4 cut(s) | 689, 761, 769, 823 |
| HhaI | GCGC | 4 cut(s) | 780, 807, 824, 834 |
| Hin1I | GRCGYC | 4 cut(s) | 700, 750, 761, 772 |
| Hin1II | CATG | 3 cut(s) | 21, 388, 899 |
| Hin6I | GCGC | 4 cut(s) | 778, 805, 822, 832 |
| HinP1I | GCGC | 4 cut(s) | 778, 805, 822, 832 |
| HinfI | GANTC | 5 cut(s) | 390, 870, 1174, 1315, 1367 |
| HpaII | CCGG | 1 cut(s) | 694 |
| HphI | GGTGA | 1 cut(s) | 158 |
| Hpy166II | GTNNAC | 3 cut(s) | 811, 904, 1248 |
| Hpy188I | TCNGA | 5 cut(s) | 199, 854, 970, 1036, 1045 |
| Hpy188III | TCNNGA | 3 cut(s) | 238, 1006, 1337 |
| Hpy8I | GTNNAC | 3 cut(s) | 811, 904, 1248 |
| Hpy99I | CGWCG | 2 cut(s) | 705, 839 |
| HpyAV | CCTTC | 2 cut(s) | 628, 1099 |
| HpyCH4III | ACNGT | 9 cut(s) | 142, 441, 562, 647, 885, 908, 922, 961, 985 |
| HpyCH4IV | ACGT | 3 cut(s) | 750, 978, 994 |
| HpyCH4V | TGCA | 4 cut(s) | 580, 811, 1208, 1297 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 769 |
| HpyF3I | CTNAG | 4 cut(s) | 29, 969, 1386, 1443 |
| HpySE526I | ACGT | 3 cut(s) | 750, 978, 994 |
| Hsp92I | GRCGYC | 4 cut(s) | 700, 750, 761, 772 |
| Hsp92II | CATG | 3 cut(s) | 21, 388, 899 |
| HspAI | GCGC | 4 cut(s) | 778, 805, 822, 832 |
| Kzo9I | GATC | 5 cut(s) | 240, 414, 912, 965, 972 |
| LmnI | GCTCC | 2 cut(s) | 676, 710 |
| LweI | GCATC | 1 cut(s) | 1284 |
| MaeI | CTAG | 5 cut(s) | 63, 228, 510, 1203, 1251 |
| MaeII | ACGT | 3 cut(s) | 750, 978, 994 |
| MaeIII | GTNAC | 7 cut(s) | 221, 746, 879, 979, 988, 1221, 1264 |
| MalI | GATC | 5 cut(s) | 242, 416, 914, 967, 974 |
| MboI | GATC | 5 cut(s) | 240, 414, 912, 965, 972 |
| MboII | GAAGA | 3 cut(s) | 421, 424, 1244 |
| MhlI | GDGCHC | 3 cut(s) | 687, 736, 813 |
| MlyI | GAGTC | 1 cut(s) | 1324 |
| MmeI | TCCRAC | 1 cut(s) | 280 |
| MnlI | CCTC | 2 cut(s) | 105, 637 |
| MseI | TTAA | 9 cut(s) | 171, 321, 329, 360, 425, 470, 573, 939, 950 |
| MspI | CCGG | 1 cut(s) | 694 |
| MspR9I | CCNGG | 1 cut(s) | 765 |
| Mva1269I | GAATGC | 1 cut(s) | 637 |
| MvaI | CCWGG | 1 cut(s) | 765 |
| MvnI | CGCG | 4 cut(s) | 807, 822, 824, 834 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 769 |
| NdeII | GATC | 5 cut(s) | 240, 414, 912, 965, 972 |
| NlaIII | CATG | 3 cut(s) | 21, 388, 899 |
| NlaIV | GGNNCC | 2 cut(s) | 672, 685 |
| NmuCI | GTSAC | 3 cut(s) | 746, 988, 1221 |
| PctI | GAATGC | 1 cut(s) | 637 |
| PfeI | GAWTC | 4 cut(s) | 390, 870, 1174, 1367 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 901 |
| PkrI | GCNGC | 1 cut(s) | 117 |
| PleI | GAGTC | 1 cut(s) | 1323 |
| PmaCI | CACGTG | 1 cut(s) | 995 |
| PmlI | CACGTG | 1 cut(s) | 995 |
| PpsI | GAGTC | 1 cut(s) | 1323 |
| Ppu21I | YACGTR | 1 cut(s) | 995 |
| PsiI | TTATAA | 1 cut(s) | 218 |
| Psp1406I | AACGTT | 1 cut(s) | 978 |
| Psp6I | CCWGG | 1 cut(s) | 763 |
| PspCI | CACGTG | 1 cut(s) | 995 |
| PspFI | CCCAGC | 1 cut(s) | 1254 |
| PspGI | CCWGG | 1 cut(s) | 763 |
| PspN4I | GGNNCC | 2 cut(s) | 672, 685 |
| PspOMI | GGGCCC | 1 cut(s) | 683 |
| PspPI | GGNCC | 4 cut(s) | 683, 684, 691, 904 |
| RsaI | GTAC | 4 cut(s) | 525, 540, 1213, 1249 |
| RsaNI | GTAC | 4 cut(s) | 524, 539, 1212, 1248 |
| SaqAI | TTAA | 9 cut(s) | 171, 321, 329, 360, 425, 470, 573, 939, 950 |
| SatI | GCNGC | 1 cut(s) | 116 |
| Sau3AI | GATC | 5 cut(s) | 240, 414, 912, 965, 972 |
| Sau96I | GGNCC | 4 cut(s) | 683, 684, 691, 904 |
| SchI | GAGTC | 1 cut(s) | 1324 |
| ScrFI | CCNGG | 1 cut(s) | 765 |
| SduI | GDGCHC | 3 cut(s) | 687, 736, 813 |
| SfaNI | GCATC | 1 cut(s) | 1284 |
| SfcI | CTRYAG | 1 cut(s) | 67 |
| SinI | GGWCC | 1 cut(s) | 904 |
| SmiI | ATTTAAAT | 2 cut(s) | 322, 330 |
| SmlI | CTYRAG | 1 cut(s) | 1337 |
| SmoI | CTYRAG | 1 cut(s) | 1337 |
| SpeI | ACTAGT | 1 cut(s) | 1202 |
| SsiI | CCGC | 2 cut(s) | 116, 820 |
| SspI | AATATT | 4 cut(s) | 326, 371, 485, 784 |
| SspMI | CTAG | 5 cut(s) | 63, 228, 510, 1203, 1251 |
| StyD4I | CCNGG | 1 cut(s) | 763 |
| StyI | CCWWGG | 2 cut(s) | 509, 687 |
| SwaI | ATTTAAAT | 2 cut(s) | 322, 330 |
| TaaI | ACNGT | 9 cut(s) | 142, 441, 562, 647, 885, 908, 922, 961, 985 |
| TaiI | ACGT | 3 cut(s) | 753, 981, 997 |
| TaqI | TCGA | 3 cut(s) | 911, 964, 1370 |
| TatI | WGTACW | 3 cut(s) | 523, 538, 1247 |
| TauI | GCSGC | 1 cut(s) | 118 |
| TfiI | GAWTC | 4 cut(s) | 390, 870, 1174, 1367 |
| Tru1I | TTAA | 9 cut(s) | 171, 321, 329, 360, 425, 470, 573, 939, 950 |
| Tru9I | TTAA | 9 cut(s) | 171, 321, 329, 360, 425, 470, 573, 939, 950 |
| TseFI | GTSAC | 3 cut(s) | 746, 988, 1221 |
| Tsp45I | GTSAC | 3 cut(s) | 746, 988, 1221 |
| TspDTI | ATGAA | 4 cut(s) | 140, 443, 1111, 1313 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 901 |
| VneI | GTGCAC | 1 cut(s) | 809 |
| VpaK11BI | GGWCC | 1 cut(s) | 904 |
| XapI | RAATTY | 3 cut(s) | 461, 946, 1019 |
| XmaJI | CCTAGG | 1 cut(s) | 509 |
| XspI | CTAG | 5 cut(s) | 63, 228, 510, 1203, 1251 |
| ZraI | GACGTC | 1 cut(s) | 751 |