FvH4_2g06391

Protein FAR1-RELATED SEQUENCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000074
Physical Location & Seq
Reverse (-)
5312302 .. 5313218
917 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g06391.t1

Sequence Viewer

Length: 711 bp
ATGTGTAGTCATTTGATCTACTTGCGCATTATCATTGGTCTGCAAGATGTCATACCCTTGTTATCTATTATCTATGTTCTGAACTTCATTGAAAGTTTACTTTTCCTGGACAAGGTGAGGACCCGCACTGAATACATATCTAGACCAGAAGACTCTGTCGATGATAAGGAAGAAACGTGTTTGTCAGAATCTGTTCAAATGAAGGATTGCACACATTACAGAGGGAAGCCCTATAAGGATCTGACATTGGAGGATTTGGTTGGTGTTGAGTTTGCAACAATTGAGGAAGTGGAAAAATTCTACTCTTCTTATTCACTTGCGAAGGGGTTTAGCATGAGGAAGCATAGGTTGGACAAGAACCGTGCAGGTACTCTAGTCACTAGAAGAGACCTCGTTTGCTCCAAGGAAGGTATGAGAGAAGAGAGTGTGAAGAAAGATGCTAAAGATTCAAGTGGGCATAACTCTAACAAGAAACGTTGCAGAGGCACCTGCAGAGTTTCAAGAGAAAATTGTCCGGCAATATTAATCGCCCGACGTTGTGCAAAAAGAGGGGTGTACTATGTAGCTGAATTCATCATTGAACACAACCATTCTTTGGCTAAACCTGAGCACCGTCAGTTTATGAGATCACACCGGCATGTAAGGGATAACGACCTTGCTCAGGTCAATGCATTGAGGAAGGTTTCAGTGAGAACATGTTGTGCTTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAR1 MULE SWIM

Protein Analysis

237

Amino Acids

27.37

Weight (kDa)

9.12

Isoelectric Point (pI)

48.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAR1 PF03101 99 - 200 4.4e-16 FAR1 DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000074)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g21861 FvH4_2g06391 FvH4_2g06392 FvH4_2g14572 FvH4_2g27932 FvH4_3g22671 FvH4_3g22671 FvH4_4g04121 FvH4_4g09442 FvH4_4g15981 FvH4_4g16251 FvH4_6g17111 FvH4_7g03431 FvH4_7g03431 FvH4_7g03431 FvH4_7g03431 FvH4_7g05771 FvH4_7g07132 FvH4_7g08581 FvH4_7g10252
prunus_persica Prupe.2G034100_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0343281 RchiOBHm_Chr1g0347821 RchiOBHm_Chr2g0100541 RchiOBHm_Chr3g0491041 RchiOBHm_Chr4g0423001 RchiOBHm_Chr4g0432011 RchiOBHm_Chr4g0434601 RchiOBHm_Chr5g0039441 RchiOBHm_Chr5g0042361 RchiOBHm_Chr5g0043531 RchiOBHm_Chr5g0058531 RchiOBHm_Chr5g0079421 RchiOBHm_Chr6g0246161 RchiOBHm_Chr6g0268121 RchiOBHm_Chr7g0189611 RchiOBHm_Chr7g0227541
rosa_laevigata RLG00000002090 RLG00000006173 RLG00000007766 RLG00000008219 RLG00000010624 RLG00000011272 RLG00000012843 RLG00000012844 RLG00000013648 RLG00000013801 RLG00000013802 RLG00000017300 RLG00000018444 RLG00000018720 RLG00000020793 RLG00000022697 RLG00000022804 RLG00000022805 RLG00000033143 RLG00000033144 RLG00000033915 RLG00000033917 RLG00000033919 RLG00000034190 RLG00000034403
rosa_multiflora Rmu_co7984152.1_g000001 Rmu_co8352885.1_g000001 Rmu_co8410147.1_g000001 Rmu_co8473903.1_g000001 Rmu_co8473903.1_g000002 Rmu_sc0000077.1_g000044 Rmu_sc0000279.1_g000050 Rmu_sc0000670.1_g000017 Rmu_sc0000837.1_g000064 Rmu_sc0000837.1_g000065 Rmu_sc0000894.1_g000068 Rmu_sc0000931.1_g000006 Rmu_sc0001103.1_g000027 Rmu_sc0001103.1_g000028 Rmu_sc0001955.1_g000017 Rmu_sc0002791.1_g000004 Rmu_sc0003047.1_g000009 Rmu_sc0003047.1_g000010 Rmu_sc0003063.1_g000011 Rmu_sc0003179.1_g000016 Rmu_sc0003179.1_g000017 Rmu_sc0003226.1_g000022 Rmu_sc0004051.1_g000001 Rmu_sc0004482.1_g000011 Rmu_sc0005394.1_g000008 Rmu_sc0005394.1_g000009 Rmu_sc0005712.1_g000027 Rmu_sc0006514.1_g000025 Rmu_sc0006514.1_g000026 Rmu_sc0006675.1_g000007 Rmu_sc0007180.1_g000003 Rmu_sc0007180.1_g000004 Rmu_sc0007877.1_g000020 Rmu_sc0011079.1_g000009 Rmu_sc0031869.1_g000001 Rmu_sc0031870.1_g000002 Rmu_ssc0000158.1_g000017 Rmu_ssc0000161.1_g000025 Rmu_ssc0000161.1_g000026 Rmu_ssc0000171.1_g000002 Rmu_ssc0000171.1_g000008 Rmu_ssc0000387.1_g000014 Rmu_ssc0000400.1_g000101
rosa_roxburghii Rroxscaffold_1G00000400 Rroxscaffold_1G00004080 Rroxscaffold_1G00009710 Rroxscaffold_1G00009720 Rroxscaffold_1G00024410 Rroxscaffold_1G00031840 Rroxscaffold_1G00040580 Rroxscaffold_1G00041710 Rroxscaffold_1G00041760 Rroxscaffold_2G00118130 Rroxscaffold_2G00118140 Rroxscaffold_2G00118640 Rroxscaffold_2G00140930 Rroxscaffold_3G00226560 Rroxscaffold_4G00313790 Rroxscaffold_4G00321730 Rroxscaffold_5G00348100 Rroxscaffold_5G00349980 Rroxscaffold_5G00350840 Rroxscaffold_7G00164330 Rroxscaffold_7G00196700
rosa_rugosa Rorug01G0094800 Rorug01G0106400 Rorug01G0106400 Rorug02G0362800 Rorug02G0564700 Rorug02G0564700 Rorug02G0570400 Rorug03G0287500 Rorug03G0329600 Rorug03G0332900 Rorug03G0333000 Rorug03G0343100 Rorug04G0031600 Rorug04G0031700 Rorug04G0031800 Rorug04G0055600 Rorug04G0065700 Rorug04G0189800 Rorug05G0103100 Rorug05G0103200 Rorug05G0106800 Rorug05G0179100 Rorug05G0179600 Rorug06G0008300 Rorug06G0184200 Rorug06G0184300 Rorug07G0181900 Rorug07G0187700 Rorug07G0283400
rosa_samantha Rh1AG090200 Rh1AG168900 Rh1CG039900 Rh1CG040000 Rh1CG055300 Rh1CG156300 Rh1DG029400 Rh1DG029500 Rh1DG029600 Rh1DG059200 Rh1DG065400 Rh1DG177000 Rh2AG354800 Rh2AG641900 Rh2AG642000 Rh2AG647200 Rh2AG647300 Rh2BG141800 Rh2BG260500 Rh2BG305300 Rh2BG336200 Rh2BG336300 Rh2BG336400 Rh2BG593800 Rh2BG593900 Rh2CG144100 Rh2CG299600 Rh2CG299700 Rh2DG143500 Rh2DG336800 Rh2DG571700 Rh2DG581400 Rh3AG246400 Rh3AG295600 Rh3BG122800 Rh3BG264600 Rh3BG310900 Rh3BG348800 Rh3CG308000 Rh3DG285900 Rh3DG304600 Rh4AG134700 Rh4AG164700 Rh4AG164800 Rh4AG164900 Rh4AG249400 Rh4AG249500 Rh4AG249600 Rh4AG249700 Rh4AG336400 Rh4AG368500 Rh4BG111500 Rh4BG130000 Rh4BG130100 Rh4BG130200 Rh4BG254800 Rh4BG254900 Rh4BG255000 Rh4BG388500 Rh4CG155300 Rh4CG155400 Rh4DG058400 Rh4DG058500 Rh4DG058600 Rh4DG059300 Rh4DG120300 Rh4DG120400 Rh4DG338900 Rh4DG374800 Rh4DG374900 Rh4DG375000 Rh5AG195100 Rh5AG266000 Rh5AG266400 Rh5AG305300 Rh5AG365400 Rh5AG375400 Rh5BG270600 Rh5CG062000 Rh5CG213600 Rh5CG229700 Rh5CG248000 Rh5CG248200 Rh5CG497300 Rh5CG497400 Rh5CG559300 Rh5DG179800 Rh5DG179900 Rh5DG239100 Rh5DG239200 Rh5DG278500 Rh5DG300000 Rh5DG300100 Rh5DG307900 Rh6AG173600 Rh6AG197900 Rh6AG315200 Rh6BG067100 Rh6BG076700 Rh6BG076800 Rh6BG101100 Rh6BG101200 Rh6BG200000 Rh6BG231600 Rh6BG324600 Rh6CG172800 Rh6CG200900 Rh6CG201000 Rh6CG235000 Rh6CG276000 Rh6CG420300 Rh6CG420400 Rh6DG498900 Rh7AG178800 Rh7AG275200 Rh7AG328700 Rh7AG328800 Rh7AG328900 Rh7AG353700 Rh7AG353800 Rh7AG367500 Rh7AG367600 Rh7AG394900 Rh7BG178500 Rh7BG321900 Rh7CG048800 Rh7CG346100 Rh7CG346200 Rh7CG346300 Rh7CG371300 Rh7CG385900 Rh7CG425400 Rh7DG106500 Rh7DG106600 Rh7DG352900 Rh7DG353000 Rh7DG361500 Rh7DG361600 Rh7DG362400 Rh7DG362500 Rh7DG363300
rosa_wichuraiana Rw0G002270 Rw0G002760 Rw0G019200 Rw1G000250 Rw1G008950 Rw1G010390 Rw1G012150 Rw1G039080 Rw2G001980 Rw2G003180 Rw2G003190 Rw4G008560 Rw4G010220 Rw4G012150 Rw5G005520 Rw5G009100 Rw5G024930 Rw5G035130 Rw5G047260 Rw5G048530 Rw6G007560 Rw6G016630 Rw6G041510 Rw7G030450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 497
Acc16I TGCGCA 1 cut(s) 26
Acc36I ACCTGC 2 cut(s) 356, 497
AccB1I GGYRCC 1 cut(s) 485
AccB7I CCANNNNNTGG 1 cut(s) 595
AciI CCGC 1 cut(s) 124
AclI AACGTT 1 cut(s) 475
AclWI GGATC 1 cut(s) 246
AcsI RAATTY 2 cut(s) 296, 569
AfaI GTAC 2 cut(s) 370, 557
AfiI CCNNNNNNNGG 3 cut(s) 112, 595, 661
AflIII ACRYGT 2 cut(s) 176, 695
AgsI TTSAA 5 cut(s) 92, 197, 450, 501, 581
AjnI CCWGG 1 cut(s) 105
AjuI GAANNNNNNNTTGG 2 cut(s) 332, 364
AluBI AGCT 1 cut(s) 566
AluI AGCT 1 cut(s) 566
Alw21I GWGCWC 1 cut(s) 612
Alw26I GTCTC 1 cut(s) 381
AlwI GGATC 1 cut(s) 246
AlwNI CAGNNNCTG 1 cut(s) 191
ApoI RAATTY 2 cut(s) 296, 569
AseI ATTAAT 1 cut(s) 524
Asp700I GAANNNNTTC 1 cut(s) 192
AspLEI GCGC 1 cut(s) 27
AspS9I GGNCC 1 cut(s) 120
AsuHPI GGTGA 1 cut(s) 127
AvaII GGWCC 1 cut(s) 120
BaeI ACNNNNGTAYC 2 cut(s) 360, 393
BanI GGYRCC 1 cut(s) 485
BbsI GAAGAC 1 cut(s) 156
Bbv12I GWGCWC 1 cut(s) 612
BciT130I CCWGG 1 cut(s) 107
BcoDI GTCTC 1 cut(s) 381
BfaI CTAG 3 cut(s) 141, 374, 381
BfmI CTRYAG 1 cut(s) 490
BfuAI ACCTGC 2 cut(s) 356, 497
Bme1390I CCNGG 1 cut(s) 107
Bme18I GGWCC 1 cut(s) 120
BmgT120I GGNCC 1 cut(s) 120
BmiI GGNNCC 2 cut(s) 122, 487
BmrFI CCNGG 1 cut(s) 107
BmsI GCATC 1 cut(s) 427
BpiI GAAGAC 1 cut(s) 156
Bpu10I CCTNAGC 2 cut(s) 606, 660
BsaI GGTCTC 1 cut(s) 381
BsaJI CCNNGG 1 cut(s) 402
Bsc4I CCNNNNNNNGG 3 cut(s) 112, 595, 661
Bse118I RCCGGY 1 cut(s) 633
BseBI CCWGG 1 cut(s) 107
BseDI CCNNGG 1 cut(s) 402
BseLI CCNNNNNNNGG 3 cut(s) 112, 595, 661
BseMII CTCAG 2 cut(s) 597, 674
BsgI GTGCAG 1 cut(s) 384
BshNI GGYRCC 1 cut(s) 485
BsiHKAI GWGCWC 1 cut(s) 612
BsiSI CCGG 2 cut(s) 515, 634
BslI CCNNNNNNNGG 3 cut(s) 112, 595, 661
BsmAI GTCTC 1 cut(s) 381
Bso31I GGTCTC 1 cut(s) 381
Bsp1286I GDGCHC 1 cut(s) 612
Bsp143I GATC 3 cut(s) 15, 238, 626
BspACI CCGC 1 cut(s) 124
BspCNI CTCAG 2 cut(s) 598, 673
BspLI GGNNCC 2 cut(s) 122, 487
BspMAI CTGCAG 1 cut(s) 494
BspMI ACCTGC 2 cut(s) 356, 497
BspPI GGATC 1 cut(s) 246
BspT107I GGYRCC 1 cut(s) 485
BspTNI GGTCTC 1 cut(s) 381
BsrFI RCCGGY 1 cut(s) 633
BssAI RCCGGY 1 cut(s) 633
BssECI CCNNGG 1 cut(s) 402
BssMI GATC 3 cut(s) 15, 238, 626
BssT1I CCWWGG 1 cut(s) 402
Bst2UI CCWGG 1 cut(s) 107
Bst4CI ACNGT 2 cut(s) 362, 614
Bst6I CTCTTC 3 cut(s) 310, 379, 414
BstDEI CTNAG 2 cut(s) 606, 660
BstENI CCTNNNNNAGG 2 cut(s) 110, 659
BstHHI GCGC 1 cut(s) 27
BstKTI GATC 3 cut(s) 18, 241, 629
BstMAI GTCTC 1 cut(s) 381
BstMBI GATC 3 cut(s) 15, 238, 626
BstNI CCWGG 1 cut(s) 107
BstNSI RCATGY 2 cut(s) 641, 699
BstSCI CCNGG 1 cut(s) 105
BstSFI CTRYAG 1 cut(s) 490
BstV2I GAAGAC 1 cut(s) 156
BstX2I RGATCY 1 cut(s) 238
BstYI RGATCY 1 cut(s) 238
BtsIMutI CAGTG 2 cut(s) 126, 693
BveI ACCTGC 2 cut(s) 356, 497
CaiI CAGNNNCTG 1 cut(s) 191
CfoI GCGC 1 cut(s) 27
Cfr10I RCCGGY 1 cut(s) 633
Cfr13I GGNCC 1 cut(s) 120
Csp6I GTAC 2 cut(s) 369, 556
CviAII CATG 3 cut(s) 334, 638, 696
CviJI RGCY 3 cut(s) 229, 566, 599
CviKI_1 RGCY 3 cut(s) 229, 566, 599
CviQI GTAC 2 cut(s) 369, 556
DdeI CTNAG 2 cut(s) 606, 660
DpnI GATC 3 cut(s) 17, 240, 628
DpnII GATC 3 cut(s) 15, 238, 626
Eam1104I CTCTTC 3 cut(s) 310, 379, 414
EarI CTCTTC 3 cut(s) 310, 379, 414
Eco130I CCWWGG 1 cut(s) 402
Eco31I GGTCTC 1 cut(s) 381
Eco47I GGWCC 1 cut(s) 120
EcoNI CCTNNNNNAGG 2 cut(s) 110, 659
EcoO109I RGGNCCY 1 cut(s) 120
EcoRI GAATTC 1 cut(s) 569
EcoRII CCWGG 1 cut(s) 105
EcoT14I CCWWGG 1 cut(s) 402
EcoT22I ATGCAT 1 cut(s) 673
ErhI CCWWGG 1 cut(s) 402
FaeI CATG 3 cut(s) 337, 641, 699
FatI CATG 3 cut(s) 333, 637, 695
FauI CCCGC 1 cut(s) 131
FspBI CTAG 3 cut(s) 141, 374, 381
FspI TGCGCA 1 cut(s) 26
GlaI GCGC 1 cut(s) 26
HapII CCGG 2 cut(s) 515, 634
HhaI GCGC 1 cut(s) 27
Hin1II CATG 3 cut(s) 337, 641, 699
Hin6I GCGC 1 cut(s) 25
HinP1I GCGC 1 cut(s) 25
HinfI GANTC 3 cut(s) 152, 188, 446
HpaII CCGG 2 cut(s) 515, 634
HphI GGTGA 1 cut(s) 127
Hpy166II GTNNAC 2 cut(s) 98, 556
Hpy188I TCNGA 3 cut(s) 81, 187, 243
Hpy188III TCNNGA 2 cut(s) 141, 501
Hpy8I GTNNAC 2 cut(s) 98, 556
Hpy99I CGWCG 1 cut(s) 537
HpyAV CCTTC 4 cut(s) 196, 316, 401, 673
HpyCH4III ACNGT 2 cut(s) 362, 614
HpyCH4IV ACGT 3 cut(s) 176, 475, 535
HpyCH4V TGCA 8 cut(s) 43, 210, 275, 365, 480, 492, 542, 671
HpyF3I CTNAG 2 cut(s) 606, 660
HpySE526I ACGT 3 cut(s) 176, 475, 535
Hsp92II CATG 3 cut(s) 337, 641, 699
HspAI GCGC 1 cut(s) 25
Kzo9I GATC 3 cut(s) 15, 238, 626
LmnI GCTCC 1 cut(s) 404
LpnPI CCDG 9 cut(s) 92, 119, 159, 351, 502, 528, 618, 647, 647
LweI GCATC 1 cut(s) 427
MaeI CTAG 3 cut(s) 141, 374, 381
MaeII ACGT 3 cut(s) 176, 475, 535
MaeIII GTNAC 1 cut(s) 376
MalI GATC 3 cut(s) 17, 240, 628
MboI GATC 3 cut(s) 15, 238, 626
MboII GAAGA 6 cut(s) 161, 182, 297, 396, 431, 442
MfeI CAATTG 1 cut(s) 279
MflI RGATCY 1 cut(s) 238
MhlI GDGCHC 1 cut(s) 612
MluCI AATT 4 cut(s) 279, 296, 508, 569
MlyI GAGTC 1 cut(s) 146
MmeI TCCRAC 1 cut(s) 330
MnlI CCTC 9 cut(s) 111, 215, 244, 277, 330, 401, 476, 542, 669
Mph1103I ATGCAT 1 cut(s) 673
MroXI GAANNNNTTC 1 cut(s) 192
MseI TTAA 1 cut(s) 524
MslI CAYNNNNRTG 1 cut(s) 636
MspI CCGG 2 cut(s) 515, 634
MspR9I CCNGG 1 cut(s) 107
MunI CAATTG 1 cut(s) 279
MvaI CCWGG 1 cut(s) 107
NdeII GATC 3 cut(s) 15, 238, 626
NlaIII CATG 3 cut(s) 337, 641, 699
NlaIV GGNNCC 2 cut(s) 122, 487
NmuCI GTSAC 1 cut(s) 376
NsbI TGCGCA 1 cut(s) 26
NsiI ATGCAT 1 cut(s) 673
NspI RCATGY 2 cut(s) 641, 699
PaqCI CACCTGC 1 cut(s) 497
PciI ACATGT 1 cut(s) 695
PdmI GAANNNNTTC 1 cut(s) 192
PfeI GAWTC 2 cut(s) 188, 446
PflFI GACNNNGTC 1 cut(s) 155
PflMI CCANNNNNTGG 1 cut(s) 595
PfoI TCCNGGA 1 cut(s) 105
PleI GAGTC 1 cut(s) 146
PpsI GAGTC 1 cut(s) 146
PpuMI RGGWCCY 1 cut(s) 120
PscI ACATGT 1 cut(s) 695
PshBI ATTAAT 1 cut(s) 524
Psp1406I AACGTT 1 cut(s) 475
Psp5II RGGWCCY 1 cut(s) 120
Psp6I CCWGG 1 cut(s) 105
PspGI CCWGG 1 cut(s) 105
PspN4I GGNNCC 2 cut(s) 122, 487
PspPI GGNCC 1 cut(s) 120
PspPPI RGGWCCY 1 cut(s) 120
PstI CTGCAG 1 cut(s) 494
PstNI CAGNNNCTG 1 cut(s) 191
PsuI RGATCY 1 cut(s) 238
PsyI GACNNNGTC 1 cut(s) 155
RsaI GTAC 2 cut(s) 370, 557
RsaNI GTAC 2 cut(s) 369, 556
RseI CAYNNNNRTG 1 cut(s) 636
SaqAI TTAA 1 cut(s) 524
Sau3AI GATC 3 cut(s) 15, 238, 626
Sau96I GGNCC 1 cut(s) 120
SchI GAGTC 1 cut(s) 146
ScrFI CCNGG 1 cut(s) 107
SduI GDGCHC 1 cut(s) 612
SfaNI GCATC 1 cut(s) 427
SfcI CTRYAG 1 cut(s) 490
SinI GGWCC 1 cut(s) 120
SmiMI CAYNNNNRTG 1 cut(s) 636
Sse9I AATT 4 cut(s) 279, 296, 508, 569
SsiI CCGC 1 cut(s) 124
SspI AATATT 1 cut(s) 522
SspMI CTAG 3 cut(s) 141, 374, 381
StyD4I CCNGG 1 cut(s) 105
StyI CCWWGG 1 cut(s) 402
TaaI ACNGT 2 cut(s) 362, 614
TaiI ACGT 3 cut(s) 179, 478, 538
TaqI TCGA 1 cut(s) 159
TasI AATT 4 cut(s) 279, 296, 508, 569
TatI WGTACW 1 cut(s) 555
TfiI GAWTC 2 cut(s) 188, 446
Tru1I TTAA 1 cut(s) 524
Tru9I TTAA 1 cut(s) 524
TscAI CASTG 2 cut(s) 133, 693
TseFI GTSAC 1 cut(s) 376
Tsp45I GTSAC 1 cut(s) 376
TspDTI ATGAA 3 cut(s) 76, 215, 562
TspRI CASTG 2 cut(s) 133, 693
Tth111I GACNNNGTC 1 cut(s) 155
Van91I CCANNNNNTGG 1 cut(s) 595
VpaK11BI GGWCC 1 cut(s) 120
VspI ATTAAT 1 cut(s) 524
XagI CCTNNNNNAGG 2 cut(s) 110, 659
XapI RAATTY 2 cut(s) 296, 569
XbaI TCTAGA 1 cut(s) 140
XceI RCATGY 2 cut(s) 641, 699
XmnI GAANNNNTTC 1 cut(s) 192
XspI CTAG 3 cut(s) 141, 374, 381
Zsp2I ATGCAT 1 cut(s) 673
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.