FvH4_3g22671

protein FAR1-RELATED SEQUENCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000074
Physical Location & Seq
Forward (+)
15700361 .. 15706627
6267 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g22671.t2

Sequence Viewer

Length: 2298 bp
ATGGACCCGAACACTGAAGAAGGATCCAATACTGACAGTTCAGATGAGGAGATTGAATACTCAAATTTGATAGAAGAGGCAGAAATGCAAGATTGTACACGATACAGAGGGAAGCTATATAAGGATTTGACATTCGAGGATTTAAAGGAAGTTGAGTTTAATAGCATCGAGGAAGTGGACAAGTTCTATTCTTACTATTCACTAGCGAAGGGGTTCAGTGTAAGGAAGTATAAGCTAGACAAGAACCGTTCTGGTACACTAGTCATTAGAAGACAGTTGGTTTGCTCAAAGCAAGGTAAGCGAAAAGAATCTGCTCCGAATAGTCATCAGCAAGGGCCAAAGGGGAAGCATGACAATTGTCAGCATTGTCAACAGGACTTGATGGGGGAGAAAAACATCAGTTGGCAGAATCCTCACAAAAAGCAGTGCACCAGACGAACAACAAGAATTAATTGTCCTGCAAGTTTCACTGTTAGGCTGTGTACCAAAAGGGGGGTATACTATGCCTCAGAATTCATAACCGAACACAACCATGCTTTGGGTAGACATGAGCATCGTCAGTTTCAAAGATCACACCCCAAGAAGCATGACCTTGCTCAGGTTAATGCATCGAGAAAAGTTTCAGCGAGAACATGCCATGCTTACGAGTTCCTAGTACACCCAGCCAGAGGCTACGAGTTTGTTCGATTATACAACAAACCACAGGGTGAAATGAAAGAAGTAGTGATGGATGGGGATGTACAATCTTTGGTAACTTGGATGAATTTCAAGGCAATGCGGGACCCCCATTTTTATGGCATATTCAGGGTGGATAAGGAGGGGAGGCTTGCAAATTTGTTTTGGCGAGATGGGCAGTCGTTAACGGACTACAATGCTTTTGGTGATATACTGATTTTTGACAACAGTGAAAAATGCAACATATATGGCAAGCCCTTGGCTGTATTCGTTGGGTGTAATAACCACAGAGCCACAGTCCTTTTTGGTTGTGCCTTGTTAGTAGATCAAACAGAGGAAACATATGATTGGGTTATTACATGCTTTCTGATCTCCATGCATGGGAAAAAACCAACTTCAGTGATCACAAATAGTGATGATGCAATAGGCAATGTGGTAACAAATTTGATACCAAAGGCTAGACACCGACTTTGTGCATGGCATGTGGGGAAAAATGGGTGTCAGAATTTGGAAGCTGTGGATACACAGAGACATTTTTTCTATTTGATATTTGCTGGATATATTAATGAGGAATGGGAAAGCGCTTGGCAGTACTTTATGACATTGAACCACCTGGAGAACAATGAATGGGTAACTAGTATGTACAATAAACGTGAAAGGTGGGCAGAGGCCTTTTTTCGAGATCATTTTTTTGGAGGAATATGCAGCACCCAACGATGTGACGAGATGCATAGAAATCTTGAAGAAGGTATTGACTGTTATATGAAGCTGTATGAGGTGTTGCCCCGGATGGAGAAAACAATTGAGCGGATAAGAAATCAGGTTCTACAGGATGATTACCGATCACAGAACTATAGCCCAGTATATGGAAACCACATGCGTTGTTTGCAGGAGGAGATTGGGAAAAAATTCACCCGTGACATATTTCTTTTAATCAAGGAGCAGATCAATTTTGAGTCTAACTTTGTTATTGGGCAGAGAGGTGTTCATCCAACAACTGGTGCATCATTGATTTCGCTGATGCAATATGGAAAGCCTGAGAAGATGTGGATTGTGTCATACCAACCTTATGAAAGCAACCCAACATTTGTGTGTTCTTGCAAACTGTTTGAGTCTGATGGAATTCCCTGTTGTCACATTTTCGCCGTCATGAAATTTGAGATGCTGACAACATTGCCAGAATCCTTGGTGAATAAAAGGTGGACGAAAGAGGCCACTGTTAAGAGGACATTATCAAGTTCTGGTTTGGTTAGTAACATGTCTGTGCAGATTGCACGGTATGGTGATTTGATGGCACAATGTGTTAAAATCTGCCATATGTCTTCCTATAGTGAGGAGGGGTACGATGAGACCAGAGAAGCTCTGAGCCAGCTGACAGTACAATCCCAAAAATACTGGGGTGGAAGTGAGTTTGAAGACAGTGTCCTTACCCAAAGCCAGCCAGGTGATTTGACTCGTCACCCCGAAGAAGCCCGGACATTTGAGTTTTTTGCTGGGTATGAGAAAAGTAAAAATGCTGCTTTGGACTCCAAAATTACTGGACTTCCAGATTTCTTTCAATACTTCAGGCCCTTTCCCAATGTGTGGCCATCTTTGCGGGCATCAGTCGCCAAGGGCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAR1 MULE SWIM

Protein Analysis

766

Amino Acids

88.84

Weight (kDa)

7.11

Isoelectric Point (pI)

43.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAR1 PF03101 61 - 180 9.2e-14 FAR1 DNA-binding domain
MULE PF10551 297 - 390 7.1e-18 MULE transposase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000074)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g21861 FvH4_2g06391 FvH4_2g06392 FvH4_2g14572 FvH4_2g27932 FvH4_3g22671 FvH4_3g22671 FvH4_4g04121 FvH4_4g09442 FvH4_4g15981 FvH4_4g16251 FvH4_6g17111 FvH4_7g03431 FvH4_7g03431 FvH4_7g03431 FvH4_7g03431 FvH4_7g05771 FvH4_7g07132 FvH4_7g08581 FvH4_7g10252
prunus_persica Prupe.2G034100_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0343281 RchiOBHm_Chr1g0347821 RchiOBHm_Chr2g0100541 RchiOBHm_Chr3g0491041 RchiOBHm_Chr4g0423001 RchiOBHm_Chr4g0432011 RchiOBHm_Chr4g0434601 RchiOBHm_Chr5g0039441 RchiOBHm_Chr5g0042361 RchiOBHm_Chr5g0043531 RchiOBHm_Chr5g0058531 RchiOBHm_Chr5g0079421 RchiOBHm_Chr6g0246161 RchiOBHm_Chr6g0268121 RchiOBHm_Chr7g0189611 RchiOBHm_Chr7g0227541
rosa_laevigata RLG00000002090 RLG00000006173 RLG00000007766 RLG00000008219 RLG00000010624 RLG00000011272 RLG00000012843 RLG00000012844 RLG00000013648 RLG00000013801 RLG00000013802 RLG00000017300 RLG00000018444 RLG00000018720 RLG00000020793 RLG00000022697 RLG00000022804 RLG00000022805 RLG00000033143 RLG00000033144 RLG00000033915 RLG00000033917 RLG00000033919 RLG00000034190 RLG00000034403
rosa_multiflora Rmu_co7984152.1_g000001 Rmu_co8352885.1_g000001 Rmu_co8410147.1_g000001 Rmu_co8473903.1_g000001 Rmu_co8473903.1_g000002 Rmu_sc0000077.1_g000044 Rmu_sc0000279.1_g000050 Rmu_sc0000670.1_g000017 Rmu_sc0000837.1_g000064 Rmu_sc0000837.1_g000065 Rmu_sc0000894.1_g000068 Rmu_sc0000931.1_g000006 Rmu_sc0001103.1_g000027 Rmu_sc0001103.1_g000028 Rmu_sc0001955.1_g000017 Rmu_sc0002791.1_g000004 Rmu_sc0003047.1_g000009 Rmu_sc0003047.1_g000010 Rmu_sc0003063.1_g000011 Rmu_sc0003179.1_g000016 Rmu_sc0003179.1_g000017 Rmu_sc0003226.1_g000022 Rmu_sc0004051.1_g000001 Rmu_sc0004482.1_g000011 Rmu_sc0005394.1_g000008 Rmu_sc0005394.1_g000009 Rmu_sc0005712.1_g000027 Rmu_sc0006514.1_g000025 Rmu_sc0006514.1_g000026 Rmu_sc0006675.1_g000007 Rmu_sc0007180.1_g000003 Rmu_sc0007180.1_g000004 Rmu_sc0007877.1_g000020 Rmu_sc0011079.1_g000009 Rmu_sc0031869.1_g000001 Rmu_sc0031870.1_g000002 Rmu_ssc0000158.1_g000017 Rmu_ssc0000161.1_g000025 Rmu_ssc0000161.1_g000026 Rmu_ssc0000171.1_g000002 Rmu_ssc0000171.1_g000008 Rmu_ssc0000387.1_g000014 Rmu_ssc0000400.1_g000101
rosa_roxburghii Rroxscaffold_1G00000400 Rroxscaffold_1G00004080 Rroxscaffold_1G00009710 Rroxscaffold_1G00009720 Rroxscaffold_1G00024410 Rroxscaffold_1G00031840 Rroxscaffold_1G00040580 Rroxscaffold_1G00041710 Rroxscaffold_1G00041760 Rroxscaffold_2G00118130 Rroxscaffold_2G00118140 Rroxscaffold_2G00118640 Rroxscaffold_2G00140930 Rroxscaffold_3G00226560 Rroxscaffold_4G00313790 Rroxscaffold_4G00321730 Rroxscaffold_5G00348100 Rroxscaffold_5G00349980 Rroxscaffold_5G00350840 Rroxscaffold_7G00164330 Rroxscaffold_7G00196700
rosa_rugosa Rorug01G0094800 Rorug01G0106400 Rorug01G0106400 Rorug02G0362800 Rorug02G0564700 Rorug02G0564700 Rorug02G0570400 Rorug03G0287500 Rorug03G0329600 Rorug03G0332900 Rorug03G0333000 Rorug03G0343100 Rorug04G0031600 Rorug04G0031700 Rorug04G0031800 Rorug04G0055600 Rorug04G0065700 Rorug04G0189800 Rorug05G0103100 Rorug05G0103200 Rorug05G0106800 Rorug05G0179100 Rorug05G0179600 Rorug06G0008300 Rorug06G0184200 Rorug06G0184300 Rorug07G0181900 Rorug07G0187700 Rorug07G0283400
rosa_samantha Rh1AG090200 Rh1AG168900 Rh1CG039900 Rh1CG040000 Rh1CG055300 Rh1CG156300 Rh1DG029400 Rh1DG029500 Rh1DG029600 Rh1DG059200 Rh1DG065400 Rh1DG177000 Rh2AG354800 Rh2AG641900 Rh2AG642000 Rh2AG647200 Rh2AG647300 Rh2BG141800 Rh2BG260500 Rh2BG305300 Rh2BG336200 Rh2BG336300 Rh2BG336400 Rh2BG593800 Rh2BG593900 Rh2CG144100 Rh2CG299600 Rh2CG299700 Rh2DG143500 Rh2DG336800 Rh2DG571700 Rh2DG581400 Rh3AG246400 Rh3AG295600 Rh3BG122800 Rh3BG264600 Rh3BG310900 Rh3BG348800 Rh3CG308000 Rh3DG285900 Rh3DG304600 Rh4AG134700 Rh4AG164700 Rh4AG164800 Rh4AG164900 Rh4AG249400 Rh4AG249500 Rh4AG249600 Rh4AG249700 Rh4AG336400 Rh4AG368500 Rh4BG111500 Rh4BG130000 Rh4BG130100 Rh4BG130200 Rh4BG254800 Rh4BG254900 Rh4BG255000 Rh4BG388500 Rh4CG155300 Rh4CG155400 Rh4DG058400 Rh4DG058500 Rh4DG058600 Rh4DG059300 Rh4DG120300 Rh4DG120400 Rh4DG338900 Rh4DG374800 Rh4DG374900 Rh4DG375000 Rh5AG195100 Rh5AG266000 Rh5AG266400 Rh5AG305300 Rh5AG365400 Rh5AG375400 Rh5BG270600 Rh5CG062000 Rh5CG213600 Rh5CG229700 Rh5CG248000 Rh5CG248200 Rh5CG497300 Rh5CG497400 Rh5CG559300 Rh5DG179800 Rh5DG179900 Rh5DG239100 Rh5DG239200 Rh5DG278500 Rh5DG300000 Rh5DG300100 Rh5DG307900 Rh6AG173600 Rh6AG197900 Rh6AG315200 Rh6BG067100 Rh6BG076700 Rh6BG076800 Rh6BG101100 Rh6BG101200 Rh6BG200000 Rh6BG231600 Rh6BG324600 Rh6CG172800 Rh6CG200900 Rh6CG201000 Rh6CG235000 Rh6CG276000 Rh6CG420300 Rh6CG420400 Rh6DG498900 Rh7AG178800 Rh7AG275200 Rh7AG328700 Rh7AG328800 Rh7AG328900 Rh7AG353700 Rh7AG353800 Rh7AG367500 Rh7AG367600 Rh7AG394900 Rh7BG178500 Rh7BG321900 Rh7CG048800 Rh7CG346100 Rh7CG346200 Rh7CG346300 Rh7CG371300 Rh7CG385900 Rh7CG425400 Rh7DG106500 Rh7DG106600 Rh7DG352900 Rh7DG353000 Rh7DG361500 Rh7DG361600 Rh7DG362400 Rh7DG362500 Rh7DG363300
rosa_wichuraiana Rw0G002270 Rw0G002760 Rw0G019200 Rw1G000250 Rw1G008950 Rw1G010390 Rw1G012150 Rw1G039080 Rw2G001980 Rw2G003180 Rw2G003190 Rw4G008560 Rw4G010220 Rw4G012150 Rw5G005520 Rw5G009100 Rw5G024930 Rw5G035130 Rw5G047260 Rw5G048530 Rw6G007560 Rw6G016630 Rw6G041510 Rw7G030450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 4 cut(s) 538, 1541, 1673, 2259
AccBSI CCGCTC 1 cut(s) 1483
AccI GTMKAC 2 cut(s) 498, 544
AciI CCGC 3 cut(s) 778, 1483, 2272
AclWI GGATC 2 cut(s) 18, 31
AcoI YGGCCR 1 cut(s) 2261
AcsI RAATTY 9 cut(s) 64, 512, 763, 832, 1117, 1180, 1583, 1797, 1829
AcuI CTGAAG 3 cut(s) 36, 1056, 2224
AdeI CACNNNGTG 2 cut(s) 707, 1976
AfaI GTAC 9 cut(s) 97, 256, 484, 657, 741, 1268, 1319, 2018, 2055
AfeI AGCGCT 1 cut(s) 1258
AfiI CCNNNNNNNGG 8 cut(s) 492, 538, 598, 668, 1056, 1541, 1673, 2259
AflIII ACRYGT 1 cut(s) 1932
AgsI TTSAA 7 cut(s) 56, 566, 769, 1282, 1418, 2090, 2234
AhlI ACTAGT 2 cut(s) 259, 1310
AjnI CCWGG 2 cut(s) 1287, 2116
AluBI AGCT 6 cut(s) 115, 235, 1190, 1444, 2036, 2047
AluI AGCT 6 cut(s) 115, 235, 1190, 1444, 2036, 2047
Alw21I GWGCWC 1 cut(s) 431
Alw26I GTCTC 2 cut(s) 1198, 2018
Alw44I GTGCAC 1 cut(s) 427
AlwI GGATC 2 cut(s) 18, 31
Aor51HI AGCGCT 1 cut(s) 1258
AoxI GGCC 5 cut(s) 335, 1344, 1887, 2243, 2261
ApaLI GTGCAC 1 cut(s) 427
ApeKI GCWGC 2 cut(s) 1380, 2192
ApoI RAATTY 9 cut(s) 64, 512, 763, 832, 1117, 1180, 1583, 1797, 1829
ArsI GACNNNNNNTTYG 4 cut(s) 264, 296, 1128, 1160
AseI ATTAAT 2 cut(s) 450, 1239
Asp700I GAANNNNTTC 3 cut(s) 212, 619, 1583
AspLEI GCGC 1 cut(s) 1259
AspS9I GGNCC 4 cut(s) 4, 335, 781, 2244
AsuC2I CCSGG 2 cut(s) 1462, 2149
AsuHPI GGTGA 7 cut(s) 719, 893, 1579, 1876, 1970, 2126, 2132
AvaII GGWCC 2 cut(s) 4, 781
BaeGI GKGCMC 1 cut(s) 431
BaeI ACNNNNGTAYC 2 cut(s) 246, 279
BalI TGGCCA 1 cut(s) 2263
BamHI GGATCC 1 cut(s) 23
BbsI GAAGAC 3 cut(s) 277, 1989, 2097
Bbv12I GWGCWC 1 cut(s) 431
BbvI GCAGC 2 cut(s) 1392, 2179
BccI CCATC 8 cut(s) 376, 721, 725, 842, 1459, 1787, 1960, 2272
BceAI ACGGC 1 cut(s) 1805
BciT130I CCWGG 2 cut(s) 1289, 2118
BciVI GTATCC 1 cut(s) 1189
BclI TGATCA 1 cut(s) 1077
BcnI CCSGG 2 cut(s) 1462, 2149
BcoDI GTCTC 2 cut(s) 1198, 2018
BcuI ACTAGT 2 cut(s) 259, 1310
BfaI CTAG 6 cut(s) 203, 236, 260, 653, 1134, 1311
BfmI CTRYAG 3 cut(s) 1502, 1528, 2002
BfoI RGCGCY 1 cut(s) 1260
BfuI GTATCC 1 cut(s) 1189
BisI GCNGC 2 cut(s) 1381, 2193
BlsI GCNGC 2 cut(s) 1382, 2194
BmcAI AGTACT 1 cut(s) 1268
Bme1390I CCNGG 4 cut(s) 1289, 1462, 2118, 2149
Bme18I GGWCC 2 cut(s) 4, 781
BmgT120I GGNCC 4 cut(s) 4, 335, 781, 2244
BmiI GGNNCC 4 cut(s) 6, 25, 782, 783
BmrFI CCNGG 4 cut(s) 1289, 1462, 2118, 2149
BmrI ACTGGG 2 cut(s) 1529, 2080
BmsI GCATC 9 cut(s) 174, 562, 617, 1084, 1392, 1686, 1688, 1827, 2285
BmuI ACTGGG 2 cut(s) 1529, 2080
BoxI GACNNNNGTC 1 cut(s) 357
BpiI GAAGAC 3 cut(s) 277, 1989, 2097
BpmI CTGGAG 1 cut(s) 1310
Bpu10I CCTNAGC 1 cut(s) 597
BpuMI CCSGG 2 cut(s) 1462, 2149
BsaI GGTCTC 1 cut(s) 2018
BsaJI CCNNGG 4 cut(s) 933, 1460, 1860, 2286
Bsc4I CCNNNNNNNGG 8 cut(s) 492, 538, 598, 668, 1056, 1541, 1673, 2259
Bse1I ACTGG 4 cut(s) 1535, 1678, 2075, 2218
Bse3DI GCAATG 3 cut(s) 780, 1111, 1847
BseBI CCWGG 2 cut(s) 1289, 2118
BseDI CCNNGG 4 cut(s) 933, 1460, 1860, 2286
BseGI GGATG 6 cut(s) 736, 742, 765, 1470, 1513, 1663
BseLI CCNNNNNNNGG 8 cut(s) 492, 538, 598, 668, 1056, 1541, 1673, 2259
BseMI GCAATG 3 cut(s) 780, 1111, 1847
BseMII CTCAG 4 cut(s) 522, 611, 1704, 2030
BseNI ACTGG 4 cut(s) 1535, 1678, 2075, 2218
BseRI GAGGAG 3 cut(s) 62, 1583, 2024
BseSI GKGCMC 1 cut(s) 431
BseXI GCAGC 2 cut(s) 1392, 2179
BseYI CCCAGC 2 cut(s) 661, 2168
BsgI GTGCAG 1 cut(s) 1961
BshFI GGCC 5 cut(s) 337, 1346, 1889, 2245, 2263
BsiHKAI GWGCWC 1 cut(s) 431
BsiSI CCGG 2 cut(s) 1462, 2149
BslFI GGGAC 1 cut(s) 794
BslI CCNNNNNNNGG 8 cut(s) 492, 538, 598, 668, 1056, 1541, 1673, 2259
BsmAI GTCTC 2 cut(s) 1198, 2018
BsmFI GGGAC 1 cut(s) 794
BsnI GGCC 5 cut(s) 337, 1346, 1889, 2245, 2263
Bso31I GGTCTC 1 cut(s) 2018
Bsp1286I GDGCHC 1 cut(s) 431
Bsp1407I TGTACA 3 cut(s) 95, 739, 1317
Bsp143I GATC 8 cut(s) 23, 569, 1000, 1044, 1077, 1357, 1517, 1620
BspACI CCGC 3 cut(s) 778, 1483, 2272
BspANI GGCC 5 cut(s) 337, 1346, 1889, 2245, 2263
BspCNI CTCAG 4 cut(s) 521, 610, 1705, 2031
BspHI TCATGA 1 cut(s) 1824
BspLI GGNNCC 4 cut(s) 6, 25, 782, 783
BspPI GGATC 2 cut(s) 18, 31
BspTNI GGTCTC 1 cut(s) 2018
BsrBI CCGCTC 1 cut(s) 1483
BsrDI GCAATG 3 cut(s) 780, 1111, 1847
BsrGI TGTACA 3 cut(s) 95, 739, 1317
BsrI ACTGG 4 cut(s) 1535, 1678, 2075, 2218
BssECI CCNNGG 4 cut(s) 933, 1460, 1860, 2286
BssMI GATC 8 cut(s) 23, 569, 1000, 1044, 1077, 1357, 1517, 1620
BssNAI GTATAC 1 cut(s) 499
BssT1I CCWWGG 3 cut(s) 933, 1860, 2286
Bst1107I GTATAC 1 cut(s) 499
Bst2UI CCWGG 2 cut(s) 1289, 2118
Bst6I CTCTTC 1 cut(s) 69
BstAUI TGTACA 3 cut(s) 95, 739, 1317
BstC8I GCNNGC 5 cut(s) 828, 929, 2045, 2114, 2274
BstDEI CTNAG 4 cut(s) 508, 597, 1713, 2039
BstENI CCTNNNNNAGG 1 cut(s) 596
BstF5I GGATG 6 cut(s) 736, 742, 765, 1470, 1513, 1663
BstH2I RGCGCY 1 cut(s) 1260
BstHHI GCGC 1 cut(s) 1259
BstKTI GATC 8 cut(s) 26, 572, 1003, 1047, 1080, 1360, 1520, 1623
BstMAI GTCTC 2 cut(s) 1198, 2018
BstMBI GATC 8 cut(s) 23, 569, 1000, 1044, 1077, 1357, 1517, 1620
BstMWI GCNNNNNNNGC 5 cut(s) 298, 850, 1561, 2269, 2282
BstNI CCWGG 2 cut(s) 1289, 2118
BstNSI RCATGY 5 cut(s) 636, 1038, 1160, 1555, 1936
BstPAI GACNNNNGTC 1 cut(s) 357
BstSCI CCNGG 4 cut(s) 1287, 1460, 2116, 2147
BstSFI CTRYAG 3 cut(s) 1502, 1528, 2002
BstSLI GKGCMC 1 cut(s) 431
BstV1I GCAGC 2 cut(s) 1392, 2179
BstV2I GAAGAC 3 cut(s) 277, 1989, 2097
BstX2I RGATCY 1 cut(s) 23
BstXI CCANNNNNNTGG 1 cut(s) 794
BstYI RGATCY 1 cut(s) 23
BstZ17I GTATAC 1 cut(s) 499
BsuI GTATCC 1 cut(s) 1189
BsuRI GGCC 5 cut(s) 337, 1346, 1889, 2245, 2263
BtsCI GGATG 6 cut(s) 736, 742, 765, 1470, 1513, 1663
BtsI GCAGTG 1 cut(s) 431
BtsIMutI CAGTG 8 cut(s) 12, 223, 431, 468, 910, 1080, 1890, 2101
Cac8I GCNNGC 5 cut(s) 828, 929, 2045, 2114, 2274
CciI TCATGA 1 cut(s) 1824
CfoI GCGC 1 cut(s) 1259
Cfr13I GGNCC 4 cut(s) 4, 335, 781, 2244
Csp6I GTAC 9 cut(s) 96, 255, 483, 656, 740, 1267, 1318, 2017, 2054
CspCI CAANNNNNGTGG 2 cut(s) 1538, 1573
CviQI GTAC 9 cut(s) 96, 255, 483, 656, 740, 1267, 1318, 2017, 2054
DdeI CTNAG 4 cut(s) 508, 597, 1713, 2039
DpnI GATC 8 cut(s) 25, 571, 1002, 1046, 1079, 1359, 1519, 1622
DpnII GATC 8 cut(s) 23, 569, 1000, 1044, 1077, 1357, 1517, 1620
DraI TTTAAA 1 cut(s) 144
DraIII CACNNNGTG 2 cut(s) 707, 1976
EaeI YGGCCR 1 cut(s) 2261
Eam1104I CTCTTC 1 cut(s) 69
EarI CTCTTC 1 cut(s) 69
Eco130I CCWWGG 3 cut(s) 933, 1860, 2286
Eco147I AGGCCT 1 cut(s) 1346
Eco31I GGTCTC 1 cut(s) 2018
Eco47I GGWCC 2 cut(s) 4, 781
Eco47III AGCGCT 1 cut(s) 1258
Eco57I CTGAAG 3 cut(s) 36, 1056, 2224
EcoNI CCTNNNNNAGG 1 cut(s) 596
EcoO109I RGGNCCY 2 cut(s) 781, 2244
EcoRI GAATTC 2 cut(s) 512, 1797
EcoRII CCWGG 2 cut(s) 1287, 2116
EcoT14I CCWWGG 3 cut(s) 933, 1860, 2286
EcoT22I ATGCAT 3 cut(s) 610, 1056, 1407
ErhI CCWWGG 3 cut(s) 933, 1860, 2286
FaqI GGGAC 1 cut(s) 794
FauI CCCGC 2 cut(s) 771, 2265
FauNDI CATATG 2 cut(s) 1018, 1992
FbaI TGATCA 1 cut(s) 1077
FblI GTMKAC 2 cut(s) 498, 544
Fnu4HI GCNGC 2 cut(s) 1381, 2193
FokI GGATG 6 cut(s) 743, 749, 772, 1477, 1520, 1650
Fsp4HI GCNGC 2 cut(s) 1381, 2193
FspBI CTAG 6 cut(s) 203, 236, 260, 653, 1134, 1311
GlaI GCGC 1 cut(s) 1258
GluI GCNGC 2 cut(s) 1381, 2193
GsaI CCCAGC 2 cut(s) 665, 2172
GsuI CTGGAG 1 cut(s) 1310
HaeII RGCGCY 1 cut(s) 1260
HaeIII GGCC 5 cut(s) 337, 1346, 1889, 2245, 2263
HapII CCGG 2 cut(s) 1462, 2149
HhaI GCGC 1 cut(s) 1259
Hin6I GCGC 1 cut(s) 1257
HinP1I GCGC 1 cut(s) 1257
HincII GTYRAC 2 cut(s) 371, 861
HindII GTYRAC 2 cut(s) 371, 861
HinfI GANTC 7 cut(s) 308, 409, 1631, 1787, 1856, 2128, 2201
HpaI GTTAAC 1 cut(s) 861
HpaII CCGG 2 cut(s) 1462, 2149
HphI GGTGA 7 cut(s) 719, 893, 1579, 1876, 1970, 2126, 2132
Hpy188I TCNGA 7 cut(s) 43, 318, 511, 1044, 1179, 1792, 2040
Hpy188III TCNNGA 5 cut(s) 612, 1355, 1415, 1825, 2222
HpyAV CCTTC 3 cut(s) 14, 202, 1415
HpyCH4IV ACGT 1 cut(s) 1327
HpyF10VI GCNNNNNNNGC 5 cut(s) 298, 850, 1561, 2269, 2282
HpyF3I CTNAG 4 cut(s) 508, 597, 1713, 2039
HpySE526I ACGT 1 cut(s) 1327
HspAI GCGC 1 cut(s) 1257
KflI GGGWCCC 1 cut(s) 781
Ksp22I TGATCA 1 cut(s) 1077
KspAI GTTAAC 1 cut(s) 861
Kzo9I GATC 8 cut(s) 23, 569, 1000, 1044, 1077, 1357, 1517, 1620
LmnI GCTCC 2 cut(s) 319, 1615
Lsp1109I GCAGC 2 cut(s) 1392, 2179
LweI GCATC 9 cut(s) 174, 562, 617, 1084, 1392, 1686, 1688, 1827, 2285
MaeI CTAG 6 cut(s) 203, 236, 260, 653, 1134, 1311
MaeII ACGT 1 cut(s) 1327
MaeIII GTNAC 8 cut(s) 751, 1111, 1306, 1394, 1592, 1808, 1928, 2132
MalI GATC 8 cut(s) 25, 571, 1002, 1046, 1079, 1359, 1519, 1622
MbiI CCGCTC 1 cut(s) 1483
MboI GATC 8 cut(s) 23, 569, 1000, 1044, 1077, 1357, 1517, 1620
MboII GAAGA 8 cut(s) 29, 86, 282, 1430, 1729, 1989, 2102, 2153
MfeI CAATTG 2 cut(s) 355, 1476
MflI RGATCY 1 cut(s) 23
MhlI GDGCHC 1 cut(s) 431
MlsI TGGCCA 1 cut(s) 2263
MluNI TGGCCA 1 cut(s) 2263
MlyI GAGTC 4 cut(s) 1640, 1796, 2122, 2195
MmeI TCCRAC 1 cut(s) 1691
Mox20I TGGCCA 1 cut(s) 2263
Mph1103I ATGCAT 3 cut(s) 610, 1056, 1407
MroXI GAANNNNTTC 3 cut(s) 212, 619, 1583
MscI TGGCCA 1 cut(s) 2263
MslI CAYNNNNRTG 4 cut(s) 531, 792, 1765, 1937
Msp20I TGGCCA 1 cut(s) 2263
MspA1I CMGCKG 1 cut(s) 2047
MspI CCGG 2 cut(s) 1462, 2149
MspR9I CCNGG 4 cut(s) 1289, 1462, 2118, 2149
MunI CAATTG 2 cut(s) 355, 1476
MvaI CCWGG 2 cut(s) 1289, 2118
MwoI GCNNNNNNNGC 5 cut(s) 298, 850, 1561, 2269, 2282
NciI CCSGG 2 cut(s) 1462, 2149
NdeI CATATG 2 cut(s) 1018, 1992
NdeII GATC 8 cut(s) 23, 569, 1000, 1044, 1077, 1357, 1517, 1620
NlaIV GGNNCC 4 cut(s) 6, 25, 782, 783
NmuCI GTSAC 4 cut(s) 1394, 1592, 1808, 2132
NsiI ATGCAT 3 cut(s) 610, 1056, 1407
NspI RCATGY 5 cut(s) 636, 1038, 1160, 1555, 1936
PagI TCATGA 1 cut(s) 1824
PceI AGGCCT 1 cut(s) 1346
PciI ACATGT 1 cut(s) 1932
PdmI GAANNNNTTC 3 cut(s) 212, 619, 1583
PfeI GAWTC 3 cut(s) 308, 409, 1856
PflFI GACNNNGTC 1 cut(s) 2096
PflMI CCANNNNNTGG 4 cut(s) 538, 1541, 1673, 2259
PkrI GCNGC 2 cut(s) 1382, 2194
PleI GAGTC 4 cut(s) 1639, 1795, 2122, 2195
PpsI GAGTC 4 cut(s) 1639, 1795, 2122, 2195
PpuMI RGGWCCY 1 cut(s) 781
PscI ACATGT 1 cut(s) 1932
PshAI GACNNNNGTC 1 cut(s) 357
PshBI ATTAAT 2 cut(s) 450, 1239
Psp5II RGGWCCY 1 cut(s) 781
Psp6I CCWGG 2 cut(s) 1287, 2116
PspFI CCCAGC 2 cut(s) 661, 2168
PspGI CCWGG 2 cut(s) 1287, 2116
PspN4I GGNNCC 4 cut(s) 6, 25, 782, 783
PspPI GGNCC 4 cut(s) 4, 335, 781, 2244
PspPPI RGGWCCY 1 cut(s) 781
PsuI RGATCY 1 cut(s) 23
PsyI GACNNNGTC 1 cut(s) 2096
PvuII CAGCTG 1 cut(s) 2047
RsaI GTAC 9 cut(s) 97, 256, 484, 657, 741, 1268, 1319, 2018, 2055
RsaNI GTAC 9 cut(s) 96, 255, 483, 656, 740, 1267, 1318, 2017, 2054
RseI CAYNNNNRTG 4 cut(s) 531, 792, 1765, 1937
SatI GCNGC 2 cut(s) 1381, 2193
Sau3AI GATC 8 cut(s) 23, 569, 1000, 1044, 1077, 1357, 1517, 1620
Sau96I GGNCC 4 cut(s) 4, 335, 781, 2244
ScaI AGTACT 1 cut(s) 1268
SchI GAGTC 4 cut(s) 1640, 1796, 2122, 2195
ScrFI CCNGG 4 cut(s) 1289, 1462, 2118, 2149
SduI GDGCHC 1 cut(s) 431
SfaNI GCATC 9 cut(s) 174, 562, 617, 1084, 1392, 1686, 1688, 1827, 2285
SfcI CTRYAG 3 cut(s) 1502, 1528, 2002
SinI GGWCC 2 cut(s) 4, 781
SmiMI CAYNNNNRTG 4 cut(s) 531, 792, 1765, 1937
SpeI ACTAGT 2 cut(s) 259, 1310
SseBI AGGCCT 1 cut(s) 1346
SsiI CCGC 3 cut(s) 778, 1483, 2272
SspMI CTAG 6 cut(s) 203, 236, 260, 653, 1134, 1311
StuI AGGCCT 1 cut(s) 1346
StyD4I CCNGG 4 cut(s) 1287, 1460, 2116, 2147
StyI CCWWGG 3 cut(s) 933, 1860, 2286
TaiI ACGT 1 cut(s) 1330
TaqI TCGA 5 cut(s) 135, 168, 611, 685, 1354
TatI WGTACW 6 cut(s) 95, 655, 739, 1266, 1317, 2053
TfiI GAWTC 3 cut(s) 308, 409, 1856
TscAI CASTG 8 cut(s) 19, 223, 431, 475, 910, 1080, 1897, 2101
TseFI GTSAC 4 cut(s) 1394, 1592, 1808, 2132
TseI GCWGC 2 cut(s) 1380, 2192
Tsp45I GTSAC 4 cut(s) 1394, 1592, 1808, 2132
TspDTI ATGAA 8 cut(s) 505, 728, 776, 1314, 1454, 1652, 1761, 1841
TspGWI ACGGA 1 cut(s) 878
TspRI CASTG 8 cut(s) 19, 223, 431, 475, 910, 1080, 1897, 2101
Tth111I GACNNNGTC 1 cut(s) 2096
Van91I CCANNNNNTGG 4 cut(s) 538, 1541, 1673, 2259
VneI GTGCAC 1 cut(s) 427
VpaK11BI GGWCC 2 cut(s) 4, 781
VspI ATTAAT 2 cut(s) 450, 1239
XagI CCTNNNNNAGG 1 cut(s) 596
XapI RAATTY 9 cut(s) 64, 512, 763, 832, 1117, 1180, 1583, 1797, 1829
XceI RCATGY 5 cut(s) 636, 1038, 1160, 1555, 1936
XmiI GTMKAC 2 cut(s) 498, 544
XmnI GAANNNNTTC 3 cut(s) 212, 619, 1583
XspI CTAG 6 cut(s) 203, 236, 260, 653, 1134, 1311
ZrmI AGTACT 1 cut(s) 1268
Zsp2I ATGCAT 3 cut(s) 610, 1056, 1407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.