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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AbsI | CCTCGAGG | 1 cut(s) | 13 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 140 |
| AciI | CCGC | 3 cut(s) | 35, 165, 317 |
| AclI | AACGTT | 1 cut(s) | 72 |
| AcsI | RAATTY | 1 cut(s) | 353 |
| AcuI | CTGAAG | 1 cut(s) | 264 |
| AfaI | GTAC | 4 cut(s) | 100, 205, 306, 493 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 140 |
| AgsI | TTSAA | 5 cut(s) | 8, 25, 261, 359, 460 |
| AjnI | CCWGG | 1 cut(s) | 181 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 160, 251 |
| Ama87I | CYCGRG | 1 cut(s) | 13 |
| ApeKI | GCWGC | 2 cut(s) | 154, 245 |
| ApoI | RAATTY | 1 cut(s) | 353 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 225 |
| AspS9I | GGNCC | 1 cut(s) | 82 |
| AsuHPI | GGTGA | 1 cut(s) | 224 |
| AvaI | CYCGRG | 1 cut(s) | 13 |
| AvaII | GGWCC | 1 cut(s) | 82 |
| BbvI | GCAGC | 2 cut(s) | 166, 257 |
| BceAI | ACGGC | 1 cut(s) | 419 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 467, 501 |
| BciT130I | CCWGG | 1 cut(s) | 183 |
| BciVI | GTATCC | 1 cut(s) | 267 |
| BfmI | CTRYAG | 1 cut(s) | 252 |
| BfuI | GTATCC | 1 cut(s) | 267 |
| BisI | GCNGC | 2 cut(s) | 155, 246 |
| BlsI | GCNGC | 2 cut(s) | 156, 247 |
| Bme1390I | CCNGG | 1 cut(s) | 183 |
| Bme18I | GGWCC | 1 cut(s) | 82 |
| BmeT110I | CYCGRG | 1 cut(s) | 13 |
| BmgT120I | GGNCC | 1 cut(s) | 82 |
| BmiI | GGNNCC | 2 cut(s) | 84, 228 |
| BmrFI | CCNGG | 1 cut(s) | 183 |
| BmsI | GCATC | 2 cut(s) | 257, 358 |
| BsaAI | YACGTR | 1 cut(s) | 98 |
| BsaJI | CCNNGG | 1 cut(s) | 342 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 140 |
| Bse118I | RCCGGY | 1 cut(s) | 488 |
| Bse1I | ACTGG | 1 cut(s) | 469 |
| BseBI | CCWGG | 1 cut(s) | 183 |
| BseDI | CCNNGG | 1 cut(s) | 342 |
| BseGI | GGATG | 1 cut(s) | 310 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 140 |
| BseMII | CTCAG | 1 cut(s) | 281 |
| BseNI | ACTGG | 1 cut(s) | 469 |
| BseXI | GCAGC | 2 cut(s) | 166, 257 |
| BseYI | CCCAGC | 1 cut(s) | 417 |
| BsgI | GTGCAG | 1 cut(s) | 279 |
| BsiHKCI | CYCGRG | 1 cut(s) | 13 |
| BsiSI | CCGG | 1 cut(s) | 489 |
| BslFI | GGGAC | 1 cut(s) | 269 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 140 |
| BsmFI | GGGAC | 1 cut(s) | 269 |
| BsoBI | CYCGRG | 1 cut(s) | 13 |
| Bsp1407I | TGTACA | 1 cut(s) | 203 |
| Bsp143I | GATC | 1 cut(s) | 334 |
| Bsp19I | CCATGG | 1 cut(s) | 342 |
| BspACI | CCGC | 3 cut(s) | 35, 165, 317 |
| BspCNI | CTCAG | 1 cut(s) | 280 |
| BspLI | GGNNCC | 2 cut(s) | 84, 228 |
| BsrFI | RCCGGY | 1 cut(s) | 488 |
| BsrGI | TGTACA | 1 cut(s) | 203 |
| BsrI | ACTGG | 1 cut(s) | 469 |
| BssAI | RCCGGY | 1 cut(s) | 488 |
| BssECI | CCNNGG | 1 cut(s) | 342 |
| BssMI | GATC | 1 cut(s) | 334 |
| BssT1I | CCWWGG | 1 cut(s) | 342 |
| Bst2UI | CCWGG | 1 cut(s) | 183 |
| Bst4CI | ACNGT | 3 cut(s) | 31, 162, 208 |
| BstAUI | TGTACA | 1 cut(s) | 203 |
| BstBAI | YACGTR | 1 cut(s) | 98 |
| BstC8I | GCNNGC | 3 cut(s) | 110, 152, 373 |
| BstDEI | CTNAG | 1 cut(s) | 267 |
| BstDSI | CCRYGG | 1 cut(s) | 342 |
| BstF5I | GGATG | 1 cut(s) | 310 |
| BstKTI | GATC | 1 cut(s) | 337 |
| BstMBI | GATC | 1 cut(s) | 334 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 245 |
| BstNI | CCWGG | 1 cut(s) | 183 |
| BstNSI | RCATGY | 1 cut(s) | 112 |
| BstSCI | CCNGG | 1 cut(s) | 181 |
| BstSFI | CTRYAG | 1 cut(s) | 252 |
| BstV1I | GCAGC | 2 cut(s) | 166, 257 |
| BsuI | GTATCC | 1 cut(s) | 267 |
| BtgI | CCRYGG | 1 cut(s) | 342 |
| BtsCI | GGATG | 1 cut(s) | 310 |
| Cac8I | GCNNGC | 3 cut(s) | 110, 152, 373 |
| CaiI | CAGNNNCTG | 2 cut(s) | 160, 251 |
| Cfr10I | RCCGGY | 1 cut(s) | 488 |
| Cfr13I | GGNCC | 1 cut(s) | 82 |
| CseI | GACGC | 1 cut(s) | 204 |
| Csp6I | GTAC | 4 cut(s) | 99, 204, 305, 492 |
| CviAII | CATG | 2 cut(s) | 109, 343 |
| CviJI | RGCY | 3 cut(s) | 265, 421, 476 |
| CviKI_1 | RGCY | 3 cut(s) | 265, 421, 476 |
| CviQI | GTAC | 4 cut(s) | 99, 204, 305, 492 |
| DdeI | CTNAG | 1 cut(s) | 267 |
| DpnI | GATC | 1 cut(s) | 336 |
| DpnII | GATC | 1 cut(s) | 334 |
| EciI | GGCGGA | 2 cut(s) | 24, 306 |
| Eco130I | CCWWGG | 1 cut(s) | 342 |
| Eco32I | GATATC | 1 cut(s) | 442 |
| Eco47I | GGWCC | 1 cut(s) | 82 |
| Eco57I | CTGAAG | 1 cut(s) | 264 |
| Eco88I | CYCGRG | 1 cut(s) | 13 |
| EcoO109I | RGGNCCY | 1 cut(s) | 82 |
| EcoRII | CCWGG | 1 cut(s) | 181 |
| EcoRV | GATATC | 1 cut(s) | 442 |
| EcoT14I | CCWWGG | 1 cut(s) | 342 |
| ErhI | CCWWGG | 1 cut(s) | 342 |
| FaeI | CATG | 2 cut(s) | 112, 346 |
| FaiI | YATR | 2 cut(s) | 110, 344 |
| FaqI | GGGAC | 1 cut(s) | 269 |
| FatI | CATG | 2 cut(s) | 108, 342 |
| Fnu4HI | GCNGC | 2 cut(s) | 155, 246 |
| FokI | GGATG | 1 cut(s) | 297 |
| Fsp4HI | GCNGC | 2 cut(s) | 155, 246 |
| GluI | GCNGC | 2 cut(s) | 155, 246 |
| GsaI | CCCAGC | 1 cut(s) | 421 |
| HapII | CCGG | 1 cut(s) | 489 |
| HgaI | GACGC | 1 cut(s) | 204 |
| Hin1II | CATG | 2 cut(s) | 112, 346 |
| HinfI | GANTC | 1 cut(s) | 257 |
| HpaII | CCGG | 1 cut(s) | 489 |
| HphI | GGTGA | 1 cut(s) | 224 |
| Hpy188I | TCNGA | 2 cut(s) | 63, 270 |
| HpyAV | CCTTC | 5 cut(s) | 73, 123, 215, 288, 432 |
| HpyCH4III | ACNGT | 3 cut(s) | 31, 162, 208 |
| HpyCH4IV | ACGT | 3 cut(s) | 72, 97, 494 |
| HpyCH4V | TGCA | 3 cut(s) | 125, 245, 296 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 245 |
| HpyF3I | CTNAG | 1 cut(s) | 267 |
| HpySE526I | ACGT | 3 cut(s) | 72, 97, 494 |
| Hsp92II | CATG | 2 cut(s) | 112, 346 |
| Kzo9I | GATC | 1 cut(s) | 334 |
| Lsp1109I | GCAGC | 2 cut(s) | 166, 257 |
| LweI | GCATC | 2 cut(s) | 257, 358 |
| MaeII | ACGT | 3 cut(s) | 72, 97, 494 |
| MaeIII | GTNAC | 1 cut(s) | 185 |
| MalI | GATC | 1 cut(s) | 336 |
| MboI | GATC | 1 cut(s) | 334 |
| MboII | GAAGA | 3 cut(s) | 230, 397, 491 |
| MluCI | AATT | 3 cut(s) | 322, 353, 395 |
| MnlI | CCTC | 7 cut(s) | 9, 22, 69, 264, 276, 318, 324 |
| MroXI | GAANNNNTTC | 1 cut(s) | 225 |
| MseI | TTAA | 1 cut(s) | 176 |
| MspI | CCGG | 1 cut(s) | 489 |
| MspR9I | CCNGG | 1 cut(s) | 183 |
| MvaI | CCWGG | 1 cut(s) | 183 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 245 |
| NcoI | CCATGG | 1 cut(s) | 342 |
| NdeII | GATC | 1 cut(s) | 334 |
| NlaIII | CATG | 2 cut(s) | 112, 346 |
| NlaIV | GGNNCC | 2 cut(s) | 84, 228 |
| NmuCI | GTSAC | 1 cut(s) | 185 |
| NspI | RCATGY | 1 cut(s) | 112 |
| PaeI | GCATGC | 1 cut(s) | 112 |
| PaeR7I | CTCGAG | 1 cut(s) | 13 |
| PdmI | GAANNNNTTC | 1 cut(s) | 225 |
| PfeI | GAWTC | 1 cut(s) | 257 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 140 |
| PkrI | GCNGC | 2 cut(s) | 156, 247 |
| Ppu21I | YACGTR | 1 cut(s) | 98 |
| PpuMI | RGGWCCY | 1 cut(s) | 82 |
| Psp1406I | AACGTT | 1 cut(s) | 72 |
| Psp5II | RGGWCCY | 1 cut(s) | 82 |
| Psp6I | CCWGG | 1 cut(s) | 181 |
| PspFI | CCCAGC | 1 cut(s) | 417 |
| PspGI | CCWGG | 1 cut(s) | 181 |
| PspN4I | GGNNCC | 2 cut(s) | 84, 228 |
| PspPI | GGNCC | 1 cut(s) | 82 |
| PspPPI | RGGWCCY | 1 cut(s) | 82 |
| PspXI | VCTCGAGB | 1 cut(s) | 13 |
| PstNI | CAGNNNCTG | 2 cut(s) | 160, 251 |
| RsaI | GTAC | 4 cut(s) | 100, 205, 306, 493 |
| RsaNI | GTAC | 4 cut(s) | 99, 204, 305, 492 |
| SaqAI | TTAA | 1 cut(s) | 176 |
| SatI | GCNGC | 2 cut(s) | 155, 246 |
| Sau3AI | GATC | 1 cut(s) | 334 |
| Sau96I | GGNCC | 1 cut(s) | 82 |
| ScrFI | CCNGG | 1 cut(s) | 183 |
| SetI | ASST | 7 cut(s) | 14, 75, 100, 280, 310, 465, 497 |
| SfaNI | GCATC | 2 cut(s) | 257, 358 |
| SfcI | CTRYAG | 1 cut(s) | 252 |
| Sfr274I | CTCGAG | 1 cut(s) | 13 |
| SinI | GGWCC | 1 cut(s) | 82 |
| SlaI | CTCGAG | 1 cut(s) | 13 |
| SmlI | CTYRAG | 1 cut(s) | 13 |
| SmoI | CTYRAG | 1 cut(s) | 13 |
| SphI | GCATGC | 1 cut(s) | 112 |
| Sse9I | AATT | 3 cut(s) | 322, 353, 395 |
| SsiI | CCGC | 3 cut(s) | 35, 165, 317 |
| StyD4I | CCNGG | 1 cut(s) | 181 |
| StyI | CCWWGG | 1 cut(s) | 342 |
| TaaI | ACNGT | 3 cut(s) | 31, 162, 208 |
| TaiI | ACGT | 3 cut(s) | 75, 100, 497 |
| TaqI | TCGA | 2 cut(s) | 14, 333 |
| TasI | AATT | 3 cut(s) | 322, 353, 395 |
| TatI | WGTACW | 1 cut(s) | 203 |
| TfiI | GAWTC | 1 cut(s) | 257 |
| Tru1I | TTAA | 1 cut(s) | 176 |
| Tru9I | TTAA | 1 cut(s) | 176 |
| TseFI | GTSAC | 1 cut(s) | 185 |
| TseI | GCWGC | 2 cut(s) | 154, 245 |
| Tsp45I | GTSAC | 1 cut(s) | 185 |
| TspDTI | ATGAA | 2 cut(s) | 231, 444 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 140 |
| VpaK11BI | GGWCC | 1 cut(s) | 82 |
| XapI | RAATTY | 1 cut(s) | 353 |
| XceI | RCATGY | 1 cut(s) | 112 |
| XhoI | CTCGAG | 1 cut(s) | 13 |
| XmnI | GAANNNNTTC | 1 cut(s) | 225 |