RchiOBHm_Chr4g0434601

Protein FAR1-RELATED SEQUENCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000074
Physical Location & Seq
Reverse (-)
58324177 .. 58324941
765 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40305

Sequence Viewer

Length: 765 bp
ATGCGGTGTATTCAACAGCAGGTTAGTGTAAGGTTTACCCACGACGTATTCCTCCTCATAAAAGATCAAATTCTTTTTGAGTCAAAGTTTCTCGTTGCTGACCGTGTTCGTTATGAGAATCAAGGATCAACACTTCATCTTGTAACCCAATATGGTAAACCTGAAAGGACATGGCATGTGACACATTATCAAGGTAAGCCCGACCTTTCTTTCGTATGCTCATGTCACCTCTTTGAGTCGGATGGTATTCCTTGCTGCCACATCTTCACAGTCATGAAGACAATGAATATGACTACCCTTCCAGATTCTTTGGTTAATCAGAGGTGGACAAAACAGGCTTGTACCAAAGATACCAGCACGCTCAGTAGTGGAATAATGCCCGCCAAGGATTTGCAGCTTGCCCGTTATGGGCAAATGATGGGTGATTGTGCTCAAATCTGTCATTCAGCTTCATTCTCAGATGAGGCATATGAAGAAATCACTAACTGTCTTAGTCGACTAATGGTTAGGTCCAAAACATTCAAGAGATGCAATGATAGTCAACCACCAGAAACTGTTGATGGTCTACACCCTAATGTGATTAGGGATCCGAACGTGTGCAAAACCAAGGGCACTCATTCCAGGCATTCCACCAGTGTTGAGGTTGAGAGGGATCAGCCAGGAGGGAGGGGTTGTGGATTTTGCAGTCGGCCAGGTCATAATGTACGCACTTGTGCACTGCTAGCTGCACAAAATGGGGGGGGGGGGGGGGGGGGGGAAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAR1 MULE SWIM

Protein Analysis

254

Amino Acids

28.24

Weight (kDa)

8.19

Isoelectric Point (pI)

41.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000074)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g21861 FvH4_2g06391 FvH4_2g06392 FvH4_2g14572 FvH4_2g27932 FvH4_3g22671 FvH4_3g22671 FvH4_4g04121 FvH4_4g09442 FvH4_4g15981 FvH4_4g16251 FvH4_6g17111 FvH4_7g03431 FvH4_7g03431 FvH4_7g03431 FvH4_7g03431 FvH4_7g05771 FvH4_7g07132 FvH4_7g08581 FvH4_7g10252
prunus_persica Prupe.2G034100_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0343281 RchiOBHm_Chr1g0347821 RchiOBHm_Chr2g0100541 RchiOBHm_Chr3g0491041 RchiOBHm_Chr4g0423001 RchiOBHm_Chr4g0432011 RchiOBHm_Chr4g0434601 RchiOBHm_Chr5g0039441 RchiOBHm_Chr5g0042361 RchiOBHm_Chr5g0043531 RchiOBHm_Chr5g0058531 RchiOBHm_Chr5g0079421 RchiOBHm_Chr6g0246161 RchiOBHm_Chr6g0268121 RchiOBHm_Chr7g0189611 RchiOBHm_Chr7g0227541
rosa_laevigata RLG00000002090 RLG00000006173 RLG00000007766 RLG00000008219 RLG00000010624 RLG00000011272 RLG00000012843 RLG00000012844 RLG00000013648 RLG00000013801 RLG00000013802 RLG00000017300 RLG00000018444 RLG00000018720 RLG00000020793 RLG00000022697 RLG00000022804 RLG00000022805 RLG00000033143 RLG00000033144 RLG00000033915 RLG00000033917 RLG00000033919 RLG00000034190 RLG00000034403
rosa_multiflora Rmu_co7984152.1_g000001 Rmu_co8352885.1_g000001 Rmu_co8410147.1_g000001 Rmu_co8473903.1_g000001 Rmu_co8473903.1_g000002 Rmu_sc0000077.1_g000044 Rmu_sc0000279.1_g000050 Rmu_sc0000670.1_g000017 Rmu_sc0000837.1_g000064 Rmu_sc0000837.1_g000065 Rmu_sc0000894.1_g000068 Rmu_sc0000931.1_g000006 Rmu_sc0001103.1_g000027 Rmu_sc0001103.1_g000028 Rmu_sc0001955.1_g000017 Rmu_sc0002791.1_g000004 Rmu_sc0003047.1_g000009 Rmu_sc0003047.1_g000010 Rmu_sc0003063.1_g000011 Rmu_sc0003179.1_g000016 Rmu_sc0003179.1_g000017 Rmu_sc0003226.1_g000022 Rmu_sc0004051.1_g000001 Rmu_sc0004482.1_g000011 Rmu_sc0005394.1_g000008 Rmu_sc0005394.1_g000009 Rmu_sc0005712.1_g000027 Rmu_sc0006514.1_g000025 Rmu_sc0006514.1_g000026 Rmu_sc0006675.1_g000007 Rmu_sc0007180.1_g000003 Rmu_sc0007180.1_g000004 Rmu_sc0007877.1_g000020 Rmu_sc0011079.1_g000009 Rmu_sc0031869.1_g000001 Rmu_sc0031870.1_g000002 Rmu_ssc0000158.1_g000017 Rmu_ssc0000161.1_g000025 Rmu_ssc0000161.1_g000026 Rmu_ssc0000171.1_g000002 Rmu_ssc0000171.1_g000008 Rmu_ssc0000387.1_g000014 Rmu_ssc0000400.1_g000101
rosa_roxburghii Rroxscaffold_1G00000400 Rroxscaffold_1G00004080 Rroxscaffold_1G00009710 Rroxscaffold_1G00009720 Rroxscaffold_1G00024410 Rroxscaffold_1G00031840 Rroxscaffold_1G00040580 Rroxscaffold_1G00041710 Rroxscaffold_1G00041760 Rroxscaffold_2G00118130 Rroxscaffold_2G00118140 Rroxscaffold_2G00118640 Rroxscaffold_2G00140930 Rroxscaffold_3G00226560 Rroxscaffold_4G00313790 Rroxscaffold_4G00321730 Rroxscaffold_5G00348100 Rroxscaffold_5G00349980 Rroxscaffold_5G00350840 Rroxscaffold_7G00164330 Rroxscaffold_7G00196700
rosa_rugosa Rorug01G0094800 Rorug01G0106400 Rorug01G0106400 Rorug02G0362800 Rorug02G0564700 Rorug02G0564700 Rorug02G0570400 Rorug03G0287500 Rorug03G0329600 Rorug03G0332900 Rorug03G0333000 Rorug03G0343100 Rorug04G0031600 Rorug04G0031700 Rorug04G0031800 Rorug04G0055600 Rorug04G0065700 Rorug04G0189800 Rorug05G0103100 Rorug05G0103200 Rorug05G0106800 Rorug05G0179100 Rorug05G0179600 Rorug06G0008300 Rorug06G0184200 Rorug06G0184300 Rorug07G0181900 Rorug07G0187700 Rorug07G0283400
rosa_samantha Rh1AG090200 Rh1AG168900 Rh1CG039900 Rh1CG040000 Rh1CG055300 Rh1CG156300 Rh1DG029400 Rh1DG029500 Rh1DG029600 Rh1DG059200 Rh1DG065400 Rh1DG177000 Rh2AG354800 Rh2AG641900 Rh2AG642000 Rh2AG647200 Rh2AG647300 Rh2BG141800 Rh2BG260500 Rh2BG305300 Rh2BG336200 Rh2BG336300 Rh2BG336400 Rh2BG593800 Rh2BG593900 Rh2CG144100 Rh2CG299600 Rh2CG299700 Rh2DG143500 Rh2DG336800 Rh2DG571700 Rh2DG581400 Rh3AG246400 Rh3AG295600 Rh3BG122800 Rh3BG264600 Rh3BG310900 Rh3BG348800 Rh3CG308000 Rh3DG285900 Rh3DG304600 Rh4AG134700 Rh4AG164700 Rh4AG164800 Rh4AG164900 Rh4AG249400 Rh4AG249500 Rh4AG249600 Rh4AG249700 Rh4AG336400 Rh4AG368500 Rh4BG111500 Rh4BG130000 Rh4BG130100 Rh4BG130200 Rh4BG254800 Rh4BG254900 Rh4BG255000 Rh4BG388500 Rh4CG155300 Rh4CG155400 Rh4DG058400 Rh4DG058500 Rh4DG058600 Rh4DG059300 Rh4DG120300 Rh4DG120400 Rh4DG338900 Rh4DG374800 Rh4DG374900 Rh4DG375000 Rh5AG195100 Rh5AG266000 Rh5AG266400 Rh5AG305300 Rh5AG365400 Rh5AG375400 Rh5BG270600 Rh5CG062000 Rh5CG213600 Rh5CG229700 Rh5CG248000 Rh5CG248200 Rh5CG497300 Rh5CG497400 Rh5CG559300 Rh5DG179800 Rh5DG179900 Rh5DG239100 Rh5DG239200 Rh5DG278500 Rh5DG300000 Rh5DG300100 Rh5DG307900 Rh6AG173600 Rh6AG197900 Rh6AG315200 Rh6BG067100 Rh6BG076700 Rh6BG076800 Rh6BG101100 Rh6BG101200 Rh6BG200000 Rh6BG231600 Rh6BG324600 Rh6CG172800 Rh6CG200900 Rh6CG201000 Rh6CG235000 Rh6CG276000 Rh6CG420300 Rh6CG420400 Rh6DG498900 Rh7AG178800 Rh7AG275200 Rh7AG328700 Rh7AG328800 Rh7AG328900 Rh7AG353700 Rh7AG353800 Rh7AG367500 Rh7AG367600 Rh7AG394900 Rh7BG178500 Rh7BG321900 Rh7CG048800 Rh7CG346100 Rh7CG346200 Rh7CG346300 Rh7CG371300 Rh7CG385900 Rh7CG425400 Rh7DG106500 Rh7DG106600 Rh7DG352900 Rh7DG353000 Rh7DG361500 Rh7DG361600 Rh7DG362400 Rh7DG362500 Rh7DG363300
rosa_wichuraiana Rw0G002270 Rw0G002760 Rw0G019200 Rw1G000250 Rw1G008950 Rw1G010390 Rw1G012150 Rw1G039080 Rw2G001980 Rw2G003180 Rw2G003190 Rw4G008560 Rw4G010220 Rw4G012150 Rw5G005520 Rw5G009100 Rw5G024930 Rw5G035130 Rw5G047260 Rw5G048530 Rw6G007560 Rw6G016630 Rw6G041510 Rw7G030450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 10
AccI GTMKAC 2 cut(s) 496, 565
AciI CCGC 2 cut(s) 4, 381
AclWI GGATC 4 cut(s) 133, 581, 594, 660
AcoI YGGCCR 1 cut(s) 689
AcsI RAATTY 1 cut(s) 69
AfaI GTAC 2 cut(s) 343, 705
AfiI CCNNNNNNNGG 1 cut(s) 408
AflIII ACRYGT 1 cut(s) 594
AgsI TTSAA 2 cut(s) 14, 523
AjnI CCWGG 3 cut(s) 620, 658, 691
AluBI AGCT 3 cut(s) 397, 449, 725
AluI AGCT 3 cut(s) 397, 449, 725
Alw21I GWGCWC 2 cut(s) 433, 718
Alw44I GTGCAC 1 cut(s) 714
AlwI GGATC 4 cut(s) 133, 581, 594, 660
AlwNI CAGNNNCTG 1 cut(s) 554
AoxI GGCC 1 cut(s) 689
ApaLI GTGCAC 1 cut(s) 714
ApeKI GCWGC 3 cut(s) 255, 394, 725
ApoI RAATTY 1 cut(s) 69
ArsI GACNNNNNNTTYG 2 cut(s) 194, 226
AspS9I GGNCC 1 cut(s) 510
AsuHPI GGTGA 2 cut(s) 218, 434
AsuNHI GCTAGC 1 cut(s) 721
AvaII GGWCC 1 cut(s) 510
BaeGI GKGCMC 2 cut(s) 614, 718
BamHI GGATCC 1 cut(s) 586
BbsI GAAGAC 1 cut(s) 284
Bbv12I GWGCWC 2 cut(s) 433, 718
BbvI GCAGC 3 cut(s) 242, 406, 712
BccI CCATC 3 cut(s) 236, 412, 554
BciT130I CCWGG 3 cut(s) 622, 660, 693
BfaI CTAG 1 cut(s) 722
BfuAI ACCTGC 1 cut(s) 10
BisI GCNGC 3 cut(s) 256, 395, 726
BlsI GCNGC 3 cut(s) 257, 396, 727
Bme1390I CCNGG 3 cut(s) 622, 660, 693
Bme18I GGWCC 1 cut(s) 510
BmgT120I GGNCC 1 cut(s) 510
BmiI GGNNCC 1 cut(s) 588
BmrFI CCNGG 3 cut(s) 622, 660, 693
BmsI GCATC 1 cut(s) 518
BmtI GCTAGC 1 cut(s) 725
BpiI GAAGAC 1 cut(s) 284
BsaJI CCNNGG 2 cut(s) 384, 606
BsaXI ACNNNNNCTCC 2 cut(s) 658, 688
Bsc4I CCNNNNNNNGG 1 cut(s) 408
Bse1I ACTGG 1 cut(s) 633
Bse3DI GCAATG 1 cut(s) 538
BseBI CCWGG 3 cut(s) 622, 660, 693
BseDI CCNNGG 2 cut(s) 384, 606
BseGI GGATG 1 cut(s) 247
BseLI CCNNNNNNNGG 1 cut(s) 408
BseMI GCAATG 1 cut(s) 538
BseMII CTCAG 2 cut(s) 376, 471
BseNI ACTGG 1 cut(s) 633
BseRI GAGGAG 1 cut(s) 44
BseSI GKGCMC 2 cut(s) 614, 718
BseXI GCAGC 3 cut(s) 242, 406, 712
BsgI GTGCAG 1 cut(s) 711
BshFI GGCC 1 cut(s) 691
BsiHKAI GWGCWC 2 cut(s) 433, 718
BslI CCNNNNNNNGG 1 cut(s) 408
BsmI GAATGC 1 cut(s) 625
BsnI GGCC 1 cut(s) 691
Bsp1286I GDGCHC 3 cut(s) 433, 614, 718
Bsp143I GATC 4 cut(s) 64, 125, 586, 652
BspACI CCGC 2 cut(s) 4, 381
BspANI GGCC 1 cut(s) 691
BspCNI CTCAG 2 cut(s) 375, 470
BspHI TCATGA 1 cut(s) 273
BspLI GGNNCC 1 cut(s) 588
BspMI ACCTGC 1 cut(s) 10
BspOI GCTAGC 1 cut(s) 725
BspPI GGATC 4 cut(s) 133, 581, 594, 660
BsrDI GCAATG 1 cut(s) 538
BsrI ACTGG 1 cut(s) 633
BssECI CCNNGG 2 cut(s) 384, 606
BssMI GATC 4 cut(s) 64, 125, 586, 652
BssT1I CCWWGG 2 cut(s) 384, 606
Bst2UI CCWGG 3 cut(s) 622, 660, 693
Bst4CI ACNGT 4 cut(s) 104, 271, 488, 556
BstC8I GCNNGC 4 cut(s) 359, 381, 399, 723
BstDEI CTNAG 3 cut(s) 362, 457, 491
BstF5I GGATG 1 cut(s) 247
BstKTI GATC 4 cut(s) 67, 128, 589, 655
BstMBI GATC 4 cut(s) 64, 125, 586, 652
BstMWI GCNNNNNNNGC 1 cut(s) 722
BstNI CCWGG 3 cut(s) 622, 660, 693
BstNSI RCATGY 1 cut(s) 179
BstSCI CCNGG 3 cut(s) 620, 658, 691
BstSLI GKGCMC 2 cut(s) 614, 718
BstV1I GCAGC 3 cut(s) 242, 406, 712
BstV2I GAAGAC 1 cut(s) 284
BstX2I RGATCY 1 cut(s) 586
BstYI RGATCY 1 cut(s) 586
BsuRI GGCC 1 cut(s) 691
BtsCI GGATG 1 cut(s) 247
BtsI GCAGTG 1 cut(s) 716
BtsIMutI CAGTG 2 cut(s) 640, 716
BveI ACCTGC 1 cut(s) 10
Cac8I GCNNGC 4 cut(s) 359, 381, 399, 723
CaiI CAGNNNCTG 1 cut(s) 554
CciI TCATGA 1 cut(s) 273
Cfr13I GGNCC 1 cut(s) 510
Csp6I GTAC 2 cut(s) 342, 704
CspCI CAANNNNNGTGG 2 cut(s) 619, 654
CviAII CATG 4 cut(s) 171, 176, 222, 274
CviJI RGCY 7 cut(s) 199, 338, 397, 449, 658, 691, 725
CviKI_1 RGCY 7 cut(s) 199, 338, 397, 449, 658, 691, 725
CviQI GTAC 2 cut(s) 342, 704
DdeI CTNAG 3 cut(s) 362, 457, 491
DpnI GATC 4 cut(s) 66, 127, 588, 654
DpnII GATC 4 cut(s) 64, 125, 586, 652
EaeI YGGCCR 1 cut(s) 689
Eco130I CCWWGG 2 cut(s) 384, 606
Eco47I GGWCC 1 cut(s) 510
EcoRII CCWGG 3 cut(s) 620, 658, 691
EcoT14I CCWWGG 2 cut(s) 384, 606
ErhI CCWWGG 2 cut(s) 384, 606
FaeI CATG 4 cut(s) 174, 179, 225, 277
FatI CATG 4 cut(s) 170, 175, 221, 273
FauI CCCGC 1 cut(s) 388
FauNDI CATATG 1 cut(s) 469
FblI GTMKAC 2 cut(s) 496, 565
Fnu4HI GCNGC 3 cut(s) 256, 395, 726
FokI GGATG 1 cut(s) 254
Fsp4HI GCNGC 3 cut(s) 256, 395, 726
FspBI CTAG 1 cut(s) 722
GluI GCNGC 3 cut(s) 256, 395, 726
HaeIII GGCC 1 cut(s) 691
Hin1II CATG 4 cut(s) 174, 179, 225, 277
HincII GTYRAC 2 cut(s) 497, 542
HindII GTYRAC 2 cut(s) 497, 542
HinfI GANTC 4 cut(s) 80, 118, 236, 305
HphI GGTGA 2 cut(s) 218, 434
Hpy166II GTNNAC 7 cut(s) 36, 158, 327, 497, 542, 566, 716
Hpy188I TCNGA 4 cut(s) 241, 321, 460, 591
Hpy188III TCNNGA 3 cut(s) 274, 302, 523
Hpy8I GTNNAC 7 cut(s) 36, 158, 327, 497, 542, 566, 716
Hpy99I CGWCG 1 cut(s) 47
HpyAV CCTTC 2 cut(s) 308, 752
HpyCH4III ACNGT 4 cut(s) 104, 271, 488, 556
HpyCH4IV ACGT 2 cut(s) 45, 594
HpyCH4V TGCA 6 cut(s) 394, 531, 600, 684, 716, 728
HpyF10VI GCNNNNNNNGC 1 cut(s) 722
HpyF3I CTNAG 3 cut(s) 362, 457, 491
HpySE526I ACGT 2 cut(s) 45, 594
Hsp92II CATG 4 cut(s) 174, 179, 225, 277
Kzo9I GATC 4 cut(s) 64, 125, 586, 652
Lsp1109I GCAGC 3 cut(s) 242, 406, 712
LweI GCATC 1 cut(s) 518
MaeI CTAG 1 cut(s) 722
MaeII ACGT 2 cut(s) 45, 594
MaeIII GTNAC 3 cut(s) 142, 178, 224
MalI GATC 4 cut(s) 66, 127, 588, 654
MboI GATC 4 cut(s) 64, 125, 586, 652
MboII GAAGA 3 cut(s) 256, 289, 485
MflI RGATCY 1 cut(s) 586
MhlI GDGCHC 3 cut(s) 433, 614, 718
MluCI AATT 1 cut(s) 69
MlyI GAGTC 2 cut(s) 89, 245
MmeI TCCRAC 1 cut(s) 219
MnlI CCTC 9 cut(s) 62, 65, 239, 315, 457, 634, 642, 656, 660
MseI TTAA 1 cut(s) 315
MslI CAYNNNNRTG 2 cut(s) 272, 573
MspR9I CCNGG 3 cut(s) 622, 660, 693
Mva1269I GAATGC 1 cut(s) 625
MvaI CCWGG 3 cut(s) 622, 660, 693
MwoI GCNNNNNNNGC 1 cut(s) 722
NdeI CATATG 1 cut(s) 469
NdeII GATC 4 cut(s) 64, 125, 586, 652
NheI GCTAGC 1 cut(s) 721
NlaIII CATG 4 cut(s) 174, 179, 225, 277
NlaIV GGNNCC 1 cut(s) 588
NmuCI GTSAC 2 cut(s) 178, 224
NspI RCATGY 1 cut(s) 179
PagI TCATGA 1 cut(s) 273
PctI GAATGC 1 cut(s) 625
PfeI GAWTC 2 cut(s) 118, 305
PkrI GCNGC 3 cut(s) 257, 396, 727
PleI GAGTC 2 cut(s) 88, 244
PpsI GAGTC 2 cut(s) 88, 244
Psp6I CCWGG 3 cut(s) 620, 658, 691
PspGI CCWGG 3 cut(s) 620, 658, 691
PspN4I GGNNCC 1 cut(s) 588
PspPI GGNCC 1 cut(s) 510
PstNI CAGNNNCTG 1 cut(s) 554
PsuI RGATCY 1 cut(s) 586
RsaI GTAC 2 cut(s) 343, 705
RsaNI GTAC 2 cut(s) 342, 704
RseI CAYNNNNRTG 2 cut(s) 272, 573
SalI GTCGAC 1 cut(s) 495
SaqAI TTAA 1 cut(s) 315
SatI GCNGC 3 cut(s) 256, 395, 726
Sau3AI GATC 4 cut(s) 64, 125, 586, 652
Sau96I GGNCC 1 cut(s) 510
SchI GAGTC 2 cut(s) 89, 245
ScrFI CCNGG 3 cut(s) 622, 660, 693
SduI GDGCHC 3 cut(s) 433, 614, 718
SfaNI GCATC 1 cut(s) 518
SinI GGWCC 1 cut(s) 510
SmiMI CAYNNNNRTG 2 cut(s) 272, 573
Sse9I AATT 1 cut(s) 69
SsiI CCGC 2 cut(s) 4, 381
SspMI CTAG 1 cut(s) 722
StyD4I CCNGG 3 cut(s) 620, 658, 691
StyI CCWWGG 2 cut(s) 384, 606
TaaI ACNGT 4 cut(s) 104, 271, 488, 556
TaiI ACGT 2 cut(s) 48, 597
TaqI TCGA 1 cut(s) 496
TasI AATT 1 cut(s) 69
TfiI GAWTC 2 cut(s) 118, 305
Tru1I TTAA 1 cut(s) 315
Tru9I TTAA 1 cut(s) 315
TscAI CASTG 2 cut(s) 640, 723
TseFI GTSAC 2 cut(s) 178, 224
TseI GCWGC 3 cut(s) 255, 394, 725
Tsp45I GTSAC 2 cut(s) 178, 224
TspDTI ATGAA 5 cut(s) 125, 290, 299, 441, 486
TspRI CASTG 2 cut(s) 640, 723
VneI GTGCAC 1 cut(s) 714
VpaK11BI GGWCC 1 cut(s) 510
XapI RAATTY 1 cut(s) 69
XceI RCATGY 1 cut(s) 179
XmiI GTMKAC 2 cut(s) 496, 565
XspI CTAG 1 cut(s) 722
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.