Rw5G024930

protein FAR1-RELATED SEQUENCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000074
Physical Location & Seq
Forward (+)
35352846 .. 35356554
3709 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G024930.1

Sequence Viewer

Length: 2274 bp
ATGGACCCAAACGCTGAAGAAGGATCCAGTACTGACAATTCGGATGAGGAGATTGAATACACAAATTTGATGGAAGAGGCAGAAATGCAAGATTGTACACGATACAGAGGGAAGCTGTATAAGGATTTGAAATTAGAGGATTTGAAGGAAGTTGAGTTTAATAGCATCGAGGAAGTGGACAAGTTCTACTCTTACTATTCTCTAGCGAAGGGGTTCAGTGTAAGGAAGCAGAAGCTCGACAAGAACCGTGCAGGACTAGTCATTAGAAGACAGTTGGTTTGCTCGAAGCAAGGGAAGCGAAGAGCCCCAAAAGATACTATTAGTCATCAGCAAGGGTCGAAGGGGAAGCAGGACAATTGTCAGCTAAGTTCAATTGGGAATTGTCAACAGAACTCGAAAAATATCAGTGGGCAGAACCCTCACAAAAAGCAGTGCACTGAAACCCGACGAGCAACAAGGGTTAATTGTCCAGCAAGTTTTGTTGTTAGGTTTTGTACCAAAAGGAGGGTGTACTATGCCTCAGAATTCATAACTGAACACAACCATGCTTTGGGTAGACCTGAGCATCGTCAGTTTCGAAGATCACACCTCAGGGACCGCAACCTTGCTCAGCTTAATGCATCAAGGAAAGTTTTAGTGAGAATATGCCATGCTTACGAGTTCTTGGTACACCAAGCTGGAGGCTACGAGTTTGTTGGATTATACAACAAACCACAGGGTGAAAGGAAAGAAGTAGTAATGGATGGGGATGCACAGTCTTTGGTAACTTGGATGAATTTCAAGGCAATGCGGGACCCCCATTTTTATTGCATATTCAGTGTTGATAAGGAGGGGAGGCTTGCAAATGTGTTTTGGCGAGATGGGCAGTCGTTAACCGATTACAATGCTTTTGGTGAGGTTCTGATTTTCGACAGCAGTGAAAAATGCAACATATATGGCAAGCCCTTGGCTGTATTCGTCGGGTGTAATAACCACAGAGCCACAGTCCTTTTTGGTTATGCATTGTTAGTAGATGAAACAAAGGAAACATATGATTGGGTGATTACATGCTTTCTGACCTCCATGCGTGGGAAAAAACCAATTTCGGTGATCACAAATAGTGATGATGCGATATGCAATGTGGTAACAAATTTGATACCAAGGGCTAGACACCGACTTTGTGCATGGCATGTCGGGAAAAATGTGTGTCAGCATTTGAAAGACGTGGATACACAGAAACACTTCTTCCATTTGATTTTTGCTGGACTTACTATTGAGGAATGGGAATTGGCTTGGCAGTACTTTGTGGCAATGAACCGGCTGGAAAACAATGAATGGGTGACTAGTATGTACAATAAACGTGAAAGGTGGGCGGAGGCCTTTTTTCGAGACCATTTTTTTGGAGGAATCTGCAGCACCCAACGATGTGACAGGATGCATAGAAATCTTGAAGAAGGTATTGACCGTTATATGAGGCTGTATGAGGTCTTGCCCCGGATGGAGAAAACAATTGAGCGGATAAGAAATCAGGTCCTACAGGATGATTACCAATCACAGAACTATGACCCTGTGTATGGCAGCCACATGCGTTGTTTGCAGGAGGAGATTGGGAAAAGGTTCACCCATGACATCTTTCTCTTAATCAAGGAGCAGATTAATTTTGAGTCCAAATTTGTTATTGGGCCGAGAAGTATTCATCCAAAAACTGGTGCGTCATTGATTTCGCTGATGCAATATGGAAAGCCTGAGCGGAGGTGGATTGTGTCGTACCAGCCTGATGAAAGCAATCCAACATTAGTGTGTTCTTGCAAACTGTTCGAGTCTGATGGAATTCCCTGTTGTCACATTTTTGCTGTCATGAAATTTGAGATGCTCACAACATTCCCAGAATCGTTGGTGAATAAAAGGTGGACAAAAGAAGCCACTGTTAAGACAGCAATATCGAGTTCCGGTTTGGTTAGTAACATGTCTGTGCAGATTGCACGGTATGGTGAATTGATGGCACAATGTGCTAAAATCTGCCATATTGCTTCCTATAGTGAGGAGGGGTACAATGAGATCAGAGAAGCTCTGAGCCAGCTGACCATACGATCCCAAAAGTACAGCAGTGAGAGTGAGTTTGAAAATGGTGTCCGTAGCCAAAGCCAGCCCATGAATTTGACTCATCACCTTGAAGAAGCCCGGGCATTTGAGTTTTTTTCTGGGTATGAGAAAAGTAAAAATGCCGCTTTGGACTCCCAAATTACTGGACTTCCAGATTTCTTTCAAAACTTCAGGCCCTTTCCCAATGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAR1 MULE SWIM

Protein Analysis

757

Amino Acids

87.94

Weight (kDa)

8.36

Isoelectric Point (pI)

45.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAR1 PF03101 61 - 121 2.7e-08 FAR1 DNA-binding domain
MULE PF10551 300 - 394 2.7e-18 MULE transposase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000074)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g21861 FvH4_2g06391 FvH4_2g06392 FvH4_2g14572 FvH4_2g27932 FvH4_3g22671 FvH4_3g22671 FvH4_4g04121 FvH4_4g09442 FvH4_4g15981 FvH4_4g16251 FvH4_6g17111 FvH4_7g03431 FvH4_7g03431 FvH4_7g03431 FvH4_7g03431 FvH4_7g05771 FvH4_7g07132 FvH4_7g08581 FvH4_7g10252
prunus_persica Prupe.2G034100_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0343281 RchiOBHm_Chr1g0347821 RchiOBHm_Chr2g0100541 RchiOBHm_Chr3g0491041 RchiOBHm_Chr4g0423001 RchiOBHm_Chr4g0432011 RchiOBHm_Chr4g0434601 RchiOBHm_Chr5g0039441 RchiOBHm_Chr5g0042361 RchiOBHm_Chr5g0043531 RchiOBHm_Chr5g0058531 RchiOBHm_Chr5g0079421 RchiOBHm_Chr6g0246161 RchiOBHm_Chr6g0268121 RchiOBHm_Chr7g0189611 RchiOBHm_Chr7g0227541
rosa_laevigata RLG00000002090 RLG00000006173 RLG00000007766 RLG00000008219 RLG00000010624 RLG00000011272 RLG00000012843 RLG00000012844 RLG00000013648 RLG00000013801 RLG00000013802 RLG00000017300 RLG00000018444 RLG00000018720 RLG00000020793 RLG00000022697 RLG00000022804 RLG00000022805 RLG00000033143 RLG00000033144 RLG00000033915 RLG00000033917 RLG00000033919 RLG00000034190 RLG00000034403
rosa_multiflora Rmu_co7984152.1_g000001 Rmu_co8352885.1_g000001 Rmu_co8410147.1_g000001 Rmu_co8473903.1_g000001 Rmu_co8473903.1_g000002 Rmu_sc0000077.1_g000044 Rmu_sc0000279.1_g000050 Rmu_sc0000670.1_g000017 Rmu_sc0000837.1_g000064 Rmu_sc0000837.1_g000065 Rmu_sc0000894.1_g000068 Rmu_sc0000931.1_g000006 Rmu_sc0001103.1_g000027 Rmu_sc0001103.1_g000028 Rmu_sc0001955.1_g000017 Rmu_sc0002791.1_g000004 Rmu_sc0003047.1_g000009 Rmu_sc0003047.1_g000010 Rmu_sc0003063.1_g000011 Rmu_sc0003179.1_g000016 Rmu_sc0003179.1_g000017 Rmu_sc0003226.1_g000022 Rmu_sc0004051.1_g000001 Rmu_sc0004482.1_g000011 Rmu_sc0005394.1_g000008 Rmu_sc0005394.1_g000009 Rmu_sc0005712.1_g000027 Rmu_sc0006514.1_g000025 Rmu_sc0006514.1_g000026 Rmu_sc0006675.1_g000007 Rmu_sc0007180.1_g000003 Rmu_sc0007180.1_g000004 Rmu_sc0007877.1_g000020 Rmu_sc0011079.1_g000009 Rmu_sc0031869.1_g000001 Rmu_sc0031870.1_g000002 Rmu_ssc0000158.1_g000017 Rmu_ssc0000161.1_g000025 Rmu_ssc0000161.1_g000026 Rmu_ssc0000171.1_g000002 Rmu_ssc0000171.1_g000008 Rmu_ssc0000387.1_g000014 Rmu_ssc0000400.1_g000101
rosa_roxburghii Rroxscaffold_1G00000400 Rroxscaffold_1G00004080 Rroxscaffold_1G00009710 Rroxscaffold_1G00009720 Rroxscaffold_1G00024410 Rroxscaffold_1G00031840 Rroxscaffold_1G00040580 Rroxscaffold_1G00041710 Rroxscaffold_1G00041760 Rroxscaffold_2G00118130 Rroxscaffold_2G00118140 Rroxscaffold_2G00118640 Rroxscaffold_2G00140930 Rroxscaffold_3G00226560 Rroxscaffold_4G00313790 Rroxscaffold_4G00321730 Rroxscaffold_5G00348100 Rroxscaffold_5G00349980 Rroxscaffold_5G00350840 Rroxscaffold_7G00164330 Rroxscaffold_7G00196700
rosa_rugosa Rorug01G0094800 Rorug01G0106400 Rorug01G0106400 Rorug02G0362800 Rorug02G0564700 Rorug02G0564700 Rorug02G0570400 Rorug03G0287500 Rorug03G0329600 Rorug03G0332900 Rorug03G0333000 Rorug03G0343100 Rorug04G0031600 Rorug04G0031700 Rorug04G0031800 Rorug04G0055600 Rorug04G0065700 Rorug04G0189800 Rorug05G0103100 Rorug05G0103200 Rorug05G0106800 Rorug05G0179100 Rorug05G0179600 Rorug06G0008300 Rorug06G0184200 Rorug06G0184300 Rorug07G0181900 Rorug07G0187700 Rorug07G0283400
rosa_samantha Rh1AG090200 Rh1AG168900 Rh1CG039900 Rh1CG040000 Rh1CG055300 Rh1CG156300 Rh1DG029400 Rh1DG029500 Rh1DG029600 Rh1DG059200 Rh1DG065400 Rh1DG177000 Rh2AG354800 Rh2AG641900 Rh2AG642000 Rh2AG647200 Rh2AG647300 Rh2BG141800 Rh2BG260500 Rh2BG305300 Rh2BG336200 Rh2BG336300 Rh2BG336400 Rh2BG593800 Rh2BG593900 Rh2CG144100 Rh2CG299600 Rh2CG299700 Rh2DG143500 Rh2DG336800 Rh2DG571700 Rh2DG581400 Rh3AG246400 Rh3AG295600 Rh3BG122800 Rh3BG264600 Rh3BG310900 Rh3BG348800 Rh3CG308000 Rh3DG285900 Rh3DG304600 Rh4AG134700 Rh4AG164700 Rh4AG164800 Rh4AG164900 Rh4AG249400 Rh4AG249500 Rh4AG249600 Rh4AG249700 Rh4AG336400 Rh4AG368500 Rh4BG111500 Rh4BG130000 Rh4BG130100 Rh4BG130200 Rh4BG254800 Rh4BG254900 Rh4BG255000 Rh4BG388500 Rh4CG155300 Rh4CG155400 Rh4DG058400 Rh4DG058500 Rh4DG058600 Rh4DG059300 Rh4DG120300 Rh4DG120400 Rh4DG338900 Rh4DG374800 Rh4DG374900 Rh4DG375000 Rh5AG195100 Rh5AG266000 Rh5AG266400 Rh5AG305300 Rh5AG365400 Rh5AG375400 Rh5BG270600 Rh5CG062000 Rh5CG213600 Rh5CG229700 Rh5CG248000 Rh5CG248200 Rh5CG497300 Rh5CG497400 Rh5CG559300 Rh5DG179800 Rh5DG179900 Rh5DG239100 Rh5DG239200 Rh5DG278500 Rh5DG300000 Rh5DG300100 Rh5DG307900 Rh6AG173600 Rh6AG197900 Rh6AG315200 Rh6BG067100 Rh6BG076700 Rh6BG076800 Rh6BG101100 Rh6BG101200 Rh6BG200000 Rh6BG231600 Rh6BG324600 Rh6CG172800 Rh6CG200900 Rh6CG201000 Rh6CG235000 Rh6CG276000 Rh6CG420300 Rh6CG420400 Rh6DG498900 Rh7AG178800 Rh7AG275200 Rh7AG328700 Rh7AG328800 Rh7AG328900 Rh7AG353700 Rh7AG353800 Rh7AG367500 Rh7AG367600 Rh7AG394900 Rh7BG178500 Rh7BG321900 Rh7CG048800 Rh7CG346100 Rh7CG346200 Rh7CG346300 Rh7CG371300 Rh7CG385900 Rh7CG425400 Rh7DG106500 Rh7DG106600 Rh7DG352900 Rh7DG353000 Rh7DG361500 Rh7DG361600 Rh7DG362400 Rh7DG362500 Rh7DG363300
rosa_wichuraiana Rw0G002270 Rw0G002760 Rw0G019200 Rw1G000250 Rw1G008950 Rw1G010390 Rw1G012150 Rw1G039080 Rw2G001980 Rw2G003180 Rw2G003190 Rw4G008560 Rw4G010220 Rw4G012150 Rw5G005520 Rw5G009100 Rw5G024930 Rw5G035130 Rw5G047260 Rw5G048530 Rw6G007560 Rw6G016630 Rw6G041510 Rw7G030450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 550, 1685
AccBSI CCGCTC 2 cut(s) 1495, 1729
AccI GTMKAC 1 cut(s) 556
AciI CCGC 6 cut(s) 598, 790, 1352, 1495, 1729, 2205
AclWI GGATC 3 cut(s) 18, 31, 2064
AcsI RAATTY 8 cut(s) 64, 524, 775, 1129, 1649, 1809, 1841, 2134
AcuI CTGAAG 2 cut(s) 36, 2236
AdeI CACNNNGTG 2 cut(s) 719, 1988
AfiI CCNNNNNNNGG 5 cut(s) 504, 550, 1068, 1553, 1685
AflIII ACRYGT 1 cut(s) 1944
AhlI ACTAGT 2 cut(s) 256, 1322
AjiI CACGTC 1 cut(s) 1204
AluBI AGCT 7 cut(s) 115, 235, 364, 613, 677, 2048, 2059
AluI AGCT 7 cut(s) 115, 235, 364, 613, 677, 2048, 2059
Alw21I GWGCWC 1 cut(s) 437
Alw26I GTCTC 1 cut(s) 1362
Alw44I GTGCAC 1 cut(s) 433
AlwI GGATC 3 cut(s) 18, 31, 2064
Ama87I CYCGRG 1 cut(s) 2160
AoxI GGCC 3 cut(s) 1356, 1661, 2255
ApaLI GTGCAC 1 cut(s) 433
ApeKI GCWGC 2 cut(s) 1392, 1557
ApoI RAATTY 8 cut(s) 64, 524, 775, 1129, 1649, 1809, 1841, 2134
ArsI GACNNNNNNTTYG 6 cut(s) 261, 293, 1140, 1172, 1361, 1393
AseI ATTAAT 1 cut(s) 1635
Asp700I GAANNNNTTC 3 cut(s) 212, 1220, 1595
AspS9I GGNCC 6 cut(s) 4, 595, 793, 1510, 1661, 2256
AsuC2I CCSGG 3 cut(s) 1474, 2161, 2162
AsuHPI GGTGA 9 cut(s) 731, 905, 1051, 1099, 1330, 1591, 1888, 1982, 2138
AsuII TTCGAA 1 cut(s) 577
AvaI CYCGRG 1 cut(s) 2160
AvaII GGWCC 4 cut(s) 4, 595, 793, 1510
AxyI CCTNAGG 1 cut(s) 590
BaeGI GKGCMC 1 cut(s) 437
BamHI GGATCC 1 cut(s) 23
BanII GRGCYC 1 cut(s) 307
BbsI GAAGAC 1 cut(s) 274
Bbv12I GWGCWC 1 cut(s) 437
BbvI GCAGC 2 cut(s) 1404, 1569
BccI CCATC 6 cut(s) 64, 737, 854, 1471, 1799, 1972
BcgI CGANNNNNNTGC 4 cut(s) 866, 900, 1777, 1811
BciVI GTATCC 1 cut(s) 1201
BclI TGATCA 1 cut(s) 1089
BcnI CCSGG 3 cut(s) 1474, 2161, 2162
BcoDI GTCTC 1 cut(s) 1362
BcuI ACTAGT 2 cut(s) 256, 1322
BfaI CTAG 4 cut(s) 203, 257, 1146, 1323
BfmI CTRYAG 3 cut(s) 1390, 1514, 2014
BfuI GTATCC 1 cut(s) 1201
BisI GCNGC 3 cut(s) 1393, 1558, 2205
BlpI GCTNAGC 1 cut(s) 609
BlsI GCNGC 3 cut(s) 1394, 1559, 2206
BmcAI AGTACT 2 cut(s) 31, 1280
Bme1390I CCNGG 3 cut(s) 1474, 2161, 2162
Bme18I GGWCC 4 cut(s) 4, 595, 793, 1510
BmeT110I CYCGRG 1 cut(s) 2160
BmgBI CACGTC 1 cut(s) 1204
BmgT120I GGNCC 6 cut(s) 4, 595, 793, 1510, 1661, 2256
BmiI GGNNCC 5 cut(s) 6, 25, 596, 794, 795
BmrFI CCNGG 3 cut(s) 1474, 2161, 2162
BmsI GCATC 8 cut(s) 174, 574, 629, 739, 1096, 1404, 1698, 1839
BoxI GACNNNNGTC 1 cut(s) 357
BpiI GAAGAC 1 cut(s) 274
BpmI CTGGAG 1 cut(s) 699
Bpu10I CCTNAGC 2 cut(s) 561, 1725
Bpu1102I GCTNAGC 1 cut(s) 609
Bpu14I TTCGAA 1 cut(s) 577
BpuMI CCSGG 3 cut(s) 1474, 2161, 2162
BsaI GGTCTC 1 cut(s) 1362
BsaJI CCNNGG 4 cut(s) 945, 1139, 1472, 2160
BsaWI WCCGGW 1 cut(s) 1928
Bsc4I CCNNNNNNNGG 5 cut(s) 504, 550, 1068, 1553, 1685
Bse118I RCCGGY 1 cut(s) 1296
Bse1I ACTGG 3 cut(s) 27, 1690, 2230
Bse21I CCTNAGG 1 cut(s) 590
Bse3DI GCAATG 3 cut(s) 792, 1123, 1296
BseDI CCNNGG 4 cut(s) 945, 1139, 1472, 2160
BseGI GGATG 8 cut(s) 49, 748, 754, 777, 1419, 1482, 1525, 1675
BseLI CCNNNNNNNGG 5 cut(s) 504, 550, 1068, 1553, 1685
BseMI GCAATG 3 cut(s) 792, 1123, 1296
BseMII CTCAG 6 cut(s) 534, 552, 604, 623, 1716, 2042
BseNI ACTGG 3 cut(s) 27, 1690, 2230
BseRI GAGGAG 3 cut(s) 62, 1595, 2036
BseSI GKGCMC 1 cut(s) 437
BseXI GCAGC 2 cut(s) 1404, 1569
BsgI GTGCAG 2 cut(s) 270, 1973
BshFI GGCC 3 cut(s) 1358, 1663, 2257
BsiHKAI GWGCWC 1 cut(s) 437
BsiHKCI CYCGRG 1 cut(s) 2160
BsiSI CCGG 4 cut(s) 1297, 1474, 1929, 2161
BslFI GGGAC 2 cut(s) 608, 806
BslI CCNNNNNNNGG 5 cut(s) 504, 550, 1068, 1553, 1685
BsmAI GTCTC 1 cut(s) 1362
BsmFI GGGAC 2 cut(s) 608, 806
BsnI GGCC 3 cut(s) 1358, 1663, 2257
Bso31I GGTCTC 1 cut(s) 1362
BsoBI CYCGRG 1 cut(s) 2160
Bsp119I TTCGAA 1 cut(s) 577
Bsp1286I GDGCHC 2 cut(s) 307, 437
Bsp1407I TGTACA 2 cut(s) 95, 1329
Bsp143I GATC 5 cut(s) 23, 581, 1089, 2037, 2069
Bsp1720I GCTNAGC 1 cut(s) 609
BspACI CCGC 6 cut(s) 598, 790, 1352, 1495, 1729, 2205
BspANI GGCC 3 cut(s) 1358, 1663, 2257
BspCNI CTCAG 6 cut(s) 533, 553, 603, 622, 1717, 2043
BspHI TCATGA 1 cut(s) 1836
BspLI GGNNCC 5 cut(s) 6, 25, 596, 794, 795
BspMAI CTGCAG 1 cut(s) 1394
BspPI GGATC 3 cut(s) 18, 31, 2064
BspQI GCTCTTC 1 cut(s) 295
BspT104I TTCGAA 1 cut(s) 577
BspTNI GGTCTC 1 cut(s) 1362
BsrBI CCGCTC 2 cut(s) 1495, 1729
BsrDI GCAATG 3 cut(s) 792, 1123, 1296
BsrFI RCCGGY 1 cut(s) 1296
BsrGI TGTACA 2 cut(s) 95, 1329
BsrI ACTGG 3 cut(s) 27, 1690, 2230
BssAI RCCGGY 1 cut(s) 1296
BssECI CCNNGG 4 cut(s) 945, 1139, 1472, 2160
BssMI GATC 5 cut(s) 23, 581, 1089, 2037, 2069
BssT1I CCWWGG 2 cut(s) 945, 1139
Bst4CI ACNGT 8 cut(s) 248, 273, 756, 985, 1445, 1794, 1906, 1965
Bst6I CTCTTC 2 cut(s) 69, 295
BstAPI GCANNNNNTGC 1 cut(s) 1988
BstAUI TGTACA 2 cut(s) 95, 1329
BstBI TTCGAA 1 cut(s) 577
BstC8I GCNNGC 4 cut(s) 840, 941, 2057, 2126
BstDEI CTNAG 7 cut(s) 365, 520, 561, 590, 609, 1725, 2051
BstF5I GGATG 8 cut(s) 49, 748, 754, 777, 1419, 1482, 1525, 1675
BstKTI GATC 5 cut(s) 26, 584, 1092, 2040, 2072
BstMAI GTCTC 1 cut(s) 1362
BstMBI GATC 5 cut(s) 23, 581, 1089, 2037, 2069
BstMWI GCNNNNNNNGC 4 cut(s) 295, 862, 1573, 1988
BstNSI RCATGY 4 cut(s) 1050, 1172, 1567, 1948
BstPAI GACNNNNGTC 1 cut(s) 357
BstSCI CCNGG 3 cut(s) 1472, 2159, 2160
BstSFI CTRYAG 3 cut(s) 1390, 1514, 2014
BstSLI GKGCMC 1 cut(s) 437
BstV1I GCAGC 2 cut(s) 1404, 1569
BstV2I GAAGAC 1 cut(s) 274
BstX2I RGATCY 1 cut(s) 23
BstXI CCANNNNNNTGG 2 cut(s) 1379, 2225
BstYI RGATCY 1 cut(s) 23
Bsu36I CCTNAGG 1 cut(s) 590
BsuI GTATCC 1 cut(s) 1201
BsuRI GGCC 3 cut(s) 1358, 1663, 2257
BtrI CACGTC 1 cut(s) 1204
BtsCI GGATG 8 cut(s) 49, 748, 754, 777, 1419, 1482, 1525, 1675
BtsI GCAGTG 3 cut(s) 437, 922, 2092
BtsIMutI CAGTG 8 cut(s) 223, 412, 435, 437, 823, 922, 1902, 2092
Cac8I GCNNGC 4 cut(s) 840, 941, 2057, 2126
CciI TCATGA 1 cut(s) 1836
Cfr10I RCCGGY 1 cut(s) 1296
Cfr13I GGNCC 6 cut(s) 4, 595, 793, 1510, 1661, 2256
Cfr9I CCCGGG 1 cut(s) 2160
CseI GACGC 1 cut(s) 1680
CspCI CAANNNNNGTGG 2 cut(s) 1550, 1585
DdeI CTNAG 7 cut(s) 365, 520, 561, 590, 609, 1725, 2051
DpnI GATC 5 cut(s) 25, 583, 1091, 2039, 2071
DpnII GATC 5 cut(s) 23, 581, 1089, 2037, 2069
DraIII CACNNNGTG 2 cut(s) 719, 1988
Eam1104I CTCTTC 2 cut(s) 69, 295
EarI CTCTTC 2 cut(s) 69, 295
EciI GGCGGA 1 cut(s) 1367
Eco130I CCWWGG 2 cut(s) 945, 1139
Eco147I AGGCCT 1 cut(s) 1358
Eco24I GRGCYC 1 cut(s) 307
Eco31I GGTCTC 1 cut(s) 1362
Eco47I GGWCC 4 cut(s) 4, 595, 793, 1510
Eco57I CTGAAG 2 cut(s) 36, 2236
Eco81I CCTNAGG 1 cut(s) 590
Eco88I CYCGRG 1 cut(s) 2160
EcoO109I RGGNCCY 3 cut(s) 793, 1510, 2256
EcoRI GAATTC 2 cut(s) 524, 1809
EcoT14I CCWWGG 2 cut(s) 945, 1139
EcoT22I ATGCAT 3 cut(s) 622, 1003, 1419
EcoT38I GRGCYC 1 cut(s) 307
ErhI CCWWGG 2 cut(s) 945, 1139
FaqI GGGAC 2 cut(s) 608, 806
FauI CCCGC 1 cut(s) 783
FauNDI CATATG 1 cut(s) 1030
FbaI TGATCA 1 cut(s) 1089
FblI GTMKAC 1 cut(s) 556
Fnu4HI GCNGC 3 cut(s) 1393, 1558, 2205
FokI GGATG 8 cut(s) 56, 755, 761, 784, 1426, 1489, 1532, 1662
FriOI GRGCYC 1 cut(s) 307
Fsp4HI GCNGC 3 cut(s) 1393, 1558, 2205
FspBI CTAG 4 cut(s) 203, 257, 1146, 1323
GluI GCNGC 3 cut(s) 1393, 1558, 2205
GsuI CTGGAG 1 cut(s) 699
HaeIII GGCC 3 cut(s) 1358, 1663, 2257
HapII CCGG 4 cut(s) 1297, 1474, 1929, 2161
HgaI GACGC 1 cut(s) 1680
HincII GTYRAC 2 cut(s) 386, 873
HindII GTYRAC 2 cut(s) 386, 873
HinfI GANTC 6 cut(s) 1386, 1643, 1799, 1868, 2140, 2213
HpaI GTTAAC 1 cut(s) 873
HpaII CCGG 4 cut(s) 1297, 1474, 1929, 2161
HphI GGTGA 9 cut(s) 731, 905, 1051, 1099, 1330, 1591, 1888, 1982, 2138
Hpy188I TCNGA 7 cut(s) 43, 523, 903, 1056, 1804, 2042, 2052
Hpy188III TCNNGA 5 cut(s) 1174, 1367, 1427, 1837, 2234
Hpy99I CGWCG 2 cut(s) 450, 962
HpyAV CCTTC 5 cut(s) 14, 139, 202, 334, 1427
HpyCH4III ACNGT 8 cut(s) 248, 273, 756, 985, 1445, 1794, 1906, 1965
HpyCH4IV ACGT 2 cut(s) 1203, 1339
HpyF10VI GCNNNNNNNGC 4 cut(s) 295, 862, 1573, 1988
HpyF3I CTNAG 7 cut(s) 365, 520, 561, 590, 609, 1725, 2051
HpySE526I ACGT 2 cut(s) 1203, 1339
KflI GGGWCCC 1 cut(s) 793
Ksp22I TGATCA 1 cut(s) 1089
KspAI GTTAAC 1 cut(s) 873
Kzo9I GATC 5 cut(s) 23, 581, 1089, 2037, 2069
LguI GCTCTTC 1 cut(s) 295
LmnI GCTCC 1 cut(s) 1627
Lsp1109I GCAGC 2 cut(s) 1404, 1569
LweI GCATC 8 cut(s) 174, 574, 629, 739, 1096, 1404, 1698, 1839
MaeI CTAG 4 cut(s) 203, 257, 1146, 1323
MaeII ACGT 2 cut(s) 1203, 1339
MaeIII GTNAC 6 cut(s) 763, 1123, 1318, 1406, 1820, 1940
MalI GATC 5 cut(s) 25, 583, 1091, 2039, 2071
MbiI CCGCTC 2 cut(s) 1495, 1729
MboI GATC 5 cut(s) 23, 581, 1089, 2037, 2069
MboII GAAGA 8 cut(s) 29, 86, 279, 312, 591, 1216, 1442, 2165
MfeI CAATTG 3 cut(s) 355, 372, 1488
MflI RGATCY 1 cut(s) 23
MhlI GDGCHC 2 cut(s) 307, 437
MlyI GAGTC 4 cut(s) 1652, 1808, 2134, 2207
MmeI TCCRAC 2 cut(s) 676, 1793
Mph1103I ATGCAT 3 cut(s) 622, 1003, 1419
MroXI GAANNNNTTC 3 cut(s) 212, 1220, 1595
MseI TTAA 7 cut(s) 159, 462, 615, 872, 1619, 1635, 1908
MslI CAYNNNNRTG 3 cut(s) 543, 1777, 1949
MspA1I CMGCKG 1 cut(s) 2059
MspI CCGG 4 cut(s) 1297, 1474, 1929, 2161
MspR9I CCNGG 3 cut(s) 1474, 2161, 2162
MunI CAATTG 3 cut(s) 355, 372, 1488
MwoI GCNNNNNNNGC 4 cut(s) 295, 862, 1573, 1988
NciI CCSGG 3 cut(s) 1474, 2161, 2162
NdeI CATATG 1 cut(s) 1030
NdeII GATC 5 cut(s) 23, 581, 1089, 2037, 2069
NlaIV GGNNCC 5 cut(s) 6, 25, 596, 794, 795
NmeAIII GCCGAG 1 cut(s) 1689
NmuCI GTSAC 3 cut(s) 1318, 1406, 1820
NsiI ATGCAT 3 cut(s) 622, 1003, 1419
NspI RCATGY 4 cut(s) 1050, 1172, 1567, 1948
NspV TTCGAA 1 cut(s) 577
PagI TCATGA 1 cut(s) 1836
PceI AGGCCT 1 cut(s) 1358
PciI ACATGT 1 cut(s) 1944
PciSI GCTCTTC 1 cut(s) 295
PcsI WCGNNNNNNNCGW 1 cut(s) 574
PdmI GAANNNNTTC 3 cut(s) 212, 1220, 1595
PfeI GAWTC 2 cut(s) 1386, 1868
PflMI CCANNNNNTGG 2 cut(s) 550, 1685
PkrI GCNGC 3 cut(s) 1394, 1559, 2206
PleI GAGTC 4 cut(s) 1651, 1807, 2134, 2207
PpsI GAGTC 4 cut(s) 1651, 1807, 2134, 2207
PpuMI RGGWCCY 2 cut(s) 793, 1510
PscI ACATGT 1 cut(s) 1944
PshAI GACNNNNGTC 1 cut(s) 357
PshBI ATTAAT 1 cut(s) 1635
Psp5II RGGWCCY 2 cut(s) 793, 1510
PspN4I GGNNCC 5 cut(s) 6, 25, 596, 794, 795
PspPI GGNCC 6 cut(s) 4, 595, 793, 1510, 1661, 2256
PspPPI RGGWCCY 2 cut(s) 793, 1510
PstI CTGCAG 1 cut(s) 1394
PsuI RGATCY 1 cut(s) 23
PvuII CAGCTG 1 cut(s) 2059
RseI CAYNNNNRTG 3 cut(s) 543, 1777, 1949
SapI GCTCTTC 1 cut(s) 295
SaqAI TTAA 7 cut(s) 159, 462, 615, 872, 1619, 1635, 1908
SatI GCNGC 3 cut(s) 1393, 1558, 2205
Sau3AI GATC 5 cut(s) 23, 581, 1089, 2037, 2069
Sau96I GGNCC 6 cut(s) 4, 595, 793, 1510, 1661, 2256
ScaI AGTACT 2 cut(s) 31, 1280
SchI GAGTC 4 cut(s) 1652, 1808, 2134, 2207
ScrFI CCNGG 3 cut(s) 1474, 2161, 2162
SduI GDGCHC 2 cut(s) 307, 437
SfaNI GCATC 8 cut(s) 174, 574, 629, 739, 1096, 1404, 1698, 1839
SfcI CTRYAG 3 cut(s) 1390, 1514, 2014
SfuI TTCGAA 1 cut(s) 577
SinI GGWCC 4 cut(s) 4, 595, 793, 1510
SmaI CCCGGG 1 cut(s) 2162
SmiMI CAYNNNNRTG 3 cut(s) 543, 1777, 1949
SpeI ACTAGT 2 cut(s) 256, 1322
SrfI GCCCGGGC 1 cut(s) 2162
SseBI AGGCCT 1 cut(s) 1358
SsiI CCGC 6 cut(s) 598, 790, 1352, 1495, 1729, 2205
SspMI CTAG 4 cut(s) 203, 257, 1146, 1323
StuI AGGCCT 1 cut(s) 1358
StyD4I CCNGG 3 cut(s) 1472, 2159, 2160
StyI CCWWGG 2 cut(s) 945, 1139
TaaI ACNGT 8 cut(s) 248, 273, 756, 985, 1445, 1794, 1906, 1965
TaiI ACGT 2 cut(s) 1206, 1342
TatI WGTACW 6 cut(s) 29, 95, 510, 1278, 1329, 2079
TauI GCSGC 1 cut(s) 2207
TfiI GAWTC 2 cut(s) 1386, 1868
Tru1I TTAA 7 cut(s) 159, 462, 615, 872, 1619, 1635, 1908
Tru9I TTAA 7 cut(s) 159, 462, 615, 872, 1619, 1635, 1908
TscAI CASTG 8 cut(s) 223, 412, 437, 442, 823, 922, 1909, 2092
TseFI GTSAC 3 cut(s) 1318, 1406, 1820
TseI GCWGC 2 cut(s) 1392, 1557
Tsp45I GTSAC 3 cut(s) 1318, 1406, 1820
TspDTI ATGAA 9 cut(s) 517, 788, 1029, 1307, 1326, 1664, 1773, 1853, 2147
TspGWI ACGGA 1 cut(s) 2102
TspMI CCCGGG 1 cut(s) 2160
TspRI CASTG 8 cut(s) 223, 412, 437, 442, 823, 922, 1909, 2092
Van91I CCANNNNNTGG 2 cut(s) 550, 1685
VneI GTGCAC 1 cut(s) 433
VpaK11BI GGWCC 4 cut(s) 4, 595, 793, 1510
VspI ATTAAT 1 cut(s) 1635
XapI RAATTY 8 cut(s) 64, 524, 775, 1129, 1649, 1809, 1841, 2134
XceI RCATGY 4 cut(s) 1050, 1172, 1567, 1948
XmaI CCCGGG 1 cut(s) 2160
XmiI GTMKAC 1 cut(s) 556
XmnI GAANNNNTTC 3 cut(s) 212, 1220, 1595
XspI CTAG 4 cut(s) 203, 257, 1146, 1323
ZrmI AGTACT 2 cut(s) 31, 1280
Zsp2I ATGCAT 3 cut(s) 622, 1003, 1419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.