Rw5G035130

Protein FAR1-RELATED SEQUENCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000074
Physical Location & Seq
Reverse (-)
60660698 .. 60663430
2733 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G035130.1

Sequence Viewer

Length: 2568 bp
ATGGATAAGAGTGGCATGCGATTCAGTGAACCGAATTACCATGGTTGGTCTGTAGATGAGGATGACTTTTCAGATGAGTACGAAGATGCAAATATGGATGAGAATCTGGATTGTCTACACTTCAATGGCAAGAAGTATGAAGATCTTAGATCCTCTGATATGATTGGTGTTGAGTTTAGCAGCGTCAAGGCAGTGGACACATTCTATTCTTACTATTCACTTGCCATGGACTTCAGTTTCCGAAAGGAAAAGTTGTGTCAAAATGCAGTACAAGTGGTGGTGAGAAGACAGTTAGTGTGTTCAAAGGAGGGTGAGAGAAAAAAAAGGGGCAAGTATGATCCTAGTTTTGCATTAGAAATCATTTCATGCGCCCAAAATCTTAGTCCACCTCATGGGACAAAACCGGTCTGCAAGAGGAAAACAGTTAACCAGAAACCAATCGTGGAAGATGCTGATACAAGAAGTATGAAACGTTCTGGGAAGCAGTTTGGTGATGGTGAACATGAAAAAAAAAGGCAGCGCCCTCAACAAAGAAATATTACTAGAGGTAATTGTCAAGCCCGTATCACAGCACGCCGATGCAAGGAAAATGGTGTCTTCCGTGTAGTCCAATTCATAACCGAACATAACCACGACCTTGCTACAACAGAGTTTGTAACAGCACTTAAGAAAGTCTCTGTTGGTACATGCAGGGCTTATGAGTTGTTGGTCCACCAAGCTGATGGCCATGAATTTGTTGGTTTCCTCATAAAAGATTTGTTTAACAAGATGGACTCTGAAAGGAAGGAACTCATGGTTGATGGTGACGCCCAGTCTACAATTAGTTTCATGAATTTGAAAGCATCTAAAGAGGCGGAGTTTTTCTGTTTGTTCAGTGTTGATACCGAAGGCCGGCTGGGTAATATGTTTTGGAGAGATACGAAGTCTATAGCAGATTACAATAATTTTGGGGATGTTTTGATTATTAATAGCACCTACAAGACCAACATATACGGCAAACCATTAGCCGTCTTTGTTGGTGCAAATAACCACAGGGTGACAGTACTATTTGCGTGTGCATTGTTGGCTGATGAGAGTGAGGACACCTACAATTGGGTGGTTACGGCCTTTTTGACTTCCATGAGGGGGAAAAAACCAATATCAGTCCTCACTGACGGGGACGAGGCATTGAGAAATGCAGTTAATGTTGTAAAAAATGTTAGGGATGCAGATGTCCAAAGGGATTTTTGCCACCTTATTTTTGCCGGACTCGGAGTTGATGAGTGGGAAAAGTCGTGGAATTACATGGTGACAATGAATGGGCTTCAAGACAACAAATGGGTTGCTGCAATGTACAACAAGATAGAGAGGTGGGCAGAAGCTTTCTTTAGGAACCATTTCTTCGGTGGTATGTGTACCACTCAAAGATGCGAGGCAATGCATAGAAATTTAAAAGGTGGGGTTGGTCGTTACATGAGGTTGTGTGAAGTGTTGCCACGCATGGACAAGACAATTGAAAGCATCAGGTTTAATGGGCTTAACGATGACTTCAAATCAATGAACTCGCACCCGGTTATTGGCAGCCACATGCGGTGTATTCAACAGCAGGTTAGTGCAAGGTTTACCCATGACGTATTCCTCCTCATAAAAGATCAAATTCTTTTTGAGTCAAAGTTTCTTGTTGCTGACCGTGTTCGTTATGAGAATCAAGTATCAACACTTCATCTTGTAACCCAATACGGTAAACCTGAAAGGACATGGCATGTGACACATTATCAAGGTAAGATCGACCTTTCTTTTGTATGCTCATGTCACCTCTTTGAGTCTGATGGTATTCCTTGCTGCCACATCTTCACAGTCATGAAGACAATGAATATGACTACCCTTCCAGAATCTTTGGTTAAACAGAGGTGGACAAAACAGGCTTGTACAAAAAATACCAGCACCCTCACTAGTGGAATAATGCCCTCCAAGGATTTGCAGCTTGCCCGTTATGGGCAAATGATGGGTGATTGTGCTCAAATCTGTCATTCGGCTTCATTCTCAGATGAGGCATATGAAGAAATTACTAACTGTCTTAGTCGACTAATGATATATAGTCAGCCACCAGAAACTGTTGATGGTTTACACCCTAATGTGATTAAGGATCTGAACGTGTGCAAAACCAAGGGCACACATTCCAGGCATTCCACCAGTGTTGAGGTTGAGAGGGATCAGCCAGGAGGGAGGGGTTGTGGATTTTGCAGCAGGCCAGGTCATAGTGTACGCACTTGTGCACTGCTAGCTGCACAAAATTGGGGGGGGGGGGGGGGGAAGGATGAACCTTGTCTCCAAAGAGCACGGAACAGCTCCATTGATGCTAGCTTGGAGTCTATTCAGGGTTCCAATAGATACAAGGGTCCAGCTGGTTTCACAAACATTGGTCAGACTGATGAGGATGATGTTTTCCTCAATCAAACTAGCAGTCAACATATGTTGCATCATGTTGAAGAATCTAGGACGTTTGACTTTTTTACTGGTCACGACACCTTTTTGGCTTCCTTGAGTGCTCCATCTGCAAATCTAAATGGTAGGAACACATTCGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAR1 MULE SWIM

Protein Analysis

855

Amino Acids

96.98

Weight (kDa)

7.13

Isoelectric Point (pI)

34.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAR1 PF03101 162 - 214 1e-07 FAR1 DNA-binding domain
ZSWIM1-3_RNaseH-like PF21056 290 - 375 9.7e-06 Zinc finger SWIM domain-containing protein 1/3, RNaseH-like domain
MULE PF10551 319 - 397 2.2e-16 MULE transposase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000074)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g21861 FvH4_2g06391 FvH4_2g06392 FvH4_2g14572 FvH4_2g27932 FvH4_3g22671 FvH4_3g22671 FvH4_4g04121 FvH4_4g09442 FvH4_4g15981 FvH4_4g16251 FvH4_6g17111 FvH4_7g03431 FvH4_7g03431 FvH4_7g03431 FvH4_7g03431 FvH4_7g05771 FvH4_7g07132 FvH4_7g08581 FvH4_7g10252
prunus_persica Prupe.2G034100_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0343281 RchiOBHm_Chr1g0347821 RchiOBHm_Chr2g0100541 RchiOBHm_Chr3g0491041 RchiOBHm_Chr4g0423001 RchiOBHm_Chr4g0432011 RchiOBHm_Chr4g0434601 RchiOBHm_Chr5g0039441 RchiOBHm_Chr5g0042361 RchiOBHm_Chr5g0043531 RchiOBHm_Chr5g0058531 RchiOBHm_Chr5g0079421 RchiOBHm_Chr6g0246161 RchiOBHm_Chr6g0268121 RchiOBHm_Chr7g0189611 RchiOBHm_Chr7g0227541
rosa_laevigata RLG00000002090 RLG00000006173 RLG00000007766 RLG00000008219 RLG00000010624 RLG00000011272 RLG00000012843 RLG00000012844 RLG00000013648 RLG00000013801 RLG00000013802 RLG00000017300 RLG00000018444 RLG00000018720 RLG00000020793 RLG00000022697 RLG00000022804 RLG00000022805 RLG00000033143 RLG00000033144 RLG00000033915 RLG00000033917 RLG00000033919 RLG00000034190 RLG00000034403
rosa_multiflora Rmu_co7984152.1_g000001 Rmu_co8352885.1_g000001 Rmu_co8410147.1_g000001 Rmu_co8473903.1_g000001 Rmu_co8473903.1_g000002 Rmu_sc0000077.1_g000044 Rmu_sc0000279.1_g000050 Rmu_sc0000670.1_g000017 Rmu_sc0000837.1_g000064 Rmu_sc0000837.1_g000065 Rmu_sc0000894.1_g000068 Rmu_sc0000931.1_g000006 Rmu_sc0001103.1_g000027 Rmu_sc0001103.1_g000028 Rmu_sc0001955.1_g000017 Rmu_sc0002791.1_g000004 Rmu_sc0003047.1_g000009 Rmu_sc0003047.1_g000010 Rmu_sc0003063.1_g000011 Rmu_sc0003179.1_g000016 Rmu_sc0003179.1_g000017 Rmu_sc0003226.1_g000022 Rmu_sc0004051.1_g000001 Rmu_sc0004482.1_g000011 Rmu_sc0005394.1_g000008 Rmu_sc0005394.1_g000009 Rmu_sc0005712.1_g000027 Rmu_sc0006514.1_g000025 Rmu_sc0006514.1_g000026 Rmu_sc0006675.1_g000007 Rmu_sc0007180.1_g000003 Rmu_sc0007180.1_g000004 Rmu_sc0007877.1_g000020 Rmu_sc0011079.1_g000009 Rmu_sc0031869.1_g000001 Rmu_sc0031870.1_g000002 Rmu_ssc0000158.1_g000017 Rmu_ssc0000161.1_g000025 Rmu_ssc0000161.1_g000026 Rmu_ssc0000171.1_g000002 Rmu_ssc0000171.1_g000008 Rmu_ssc0000387.1_g000014 Rmu_ssc0000400.1_g000101
rosa_roxburghii Rroxscaffold_1G00000400 Rroxscaffold_1G00004080 Rroxscaffold_1G00009710 Rroxscaffold_1G00009720 Rroxscaffold_1G00024410 Rroxscaffold_1G00031840 Rroxscaffold_1G00040580 Rroxscaffold_1G00041710 Rroxscaffold_1G00041760 Rroxscaffold_2G00118130 Rroxscaffold_2G00118140 Rroxscaffold_2G00118640 Rroxscaffold_2G00140930 Rroxscaffold_3G00226560 Rroxscaffold_4G00313790 Rroxscaffold_4G00321730 Rroxscaffold_5G00348100 Rroxscaffold_5G00349980 Rroxscaffold_5G00350840 Rroxscaffold_7G00164330 Rroxscaffold_7G00196700
rosa_rugosa Rorug01G0094800 Rorug01G0106400 Rorug01G0106400 Rorug02G0362800 Rorug02G0564700 Rorug02G0564700 Rorug02G0570400 Rorug03G0287500 Rorug03G0329600 Rorug03G0332900 Rorug03G0333000 Rorug03G0343100 Rorug04G0031600 Rorug04G0031700 Rorug04G0031800 Rorug04G0055600 Rorug04G0065700 Rorug04G0189800 Rorug05G0103100 Rorug05G0103200 Rorug05G0106800 Rorug05G0179100 Rorug05G0179600 Rorug06G0008300 Rorug06G0184200 Rorug06G0184300 Rorug07G0181900 Rorug07G0187700 Rorug07G0283400
rosa_samantha Rh1AG090200 Rh1AG168900 Rh1CG039900 Rh1CG040000 Rh1CG055300 Rh1CG156300 Rh1DG029400 Rh1DG029500 Rh1DG029600 Rh1DG059200 Rh1DG065400 Rh1DG177000 Rh2AG354800 Rh2AG641900 Rh2AG642000 Rh2AG647200 Rh2AG647300 Rh2BG141800 Rh2BG260500 Rh2BG305300 Rh2BG336200 Rh2BG336300 Rh2BG336400 Rh2BG593800 Rh2BG593900 Rh2CG144100 Rh2CG299600 Rh2CG299700 Rh2DG143500 Rh2DG336800 Rh2DG571700 Rh2DG581400 Rh3AG246400 Rh3AG295600 Rh3BG122800 Rh3BG264600 Rh3BG310900 Rh3BG348800 Rh3CG308000 Rh3DG285900 Rh3DG304600 Rh4AG134700 Rh4AG164700 Rh4AG164800 Rh4AG164900 Rh4AG249400 Rh4AG249500 Rh4AG249600 Rh4AG249700 Rh4AG336400 Rh4AG368500 Rh4BG111500 Rh4BG130000 Rh4BG130100 Rh4BG130200 Rh4BG254800 Rh4BG254900 Rh4BG255000 Rh4BG388500 Rh4CG155300 Rh4CG155400 Rh4DG058400 Rh4DG058500 Rh4DG058600 Rh4DG059300 Rh4DG120300 Rh4DG120400 Rh4DG338900 Rh4DG374800 Rh4DG374900 Rh4DG375000 Rh5AG195100 Rh5AG266000 Rh5AG266400 Rh5AG305300 Rh5AG365400 Rh5AG375400 Rh5BG270600 Rh5CG062000 Rh5CG213600 Rh5CG229700 Rh5CG248000 Rh5CG248200 Rh5CG497300 Rh5CG497400 Rh5CG559300 Rh5DG179800 Rh5DG179900 Rh5DG239100 Rh5DG239200 Rh5DG278500 Rh5DG300000 Rh5DG300100 Rh5DG307900 Rh6AG173600 Rh6AG197900 Rh6AG315200 Rh6BG067100 Rh6BG076700 Rh6BG076800 Rh6BG101100 Rh6BG101200 Rh6BG200000 Rh6BG231600 Rh6BG324600 Rh6CG172800 Rh6CG200900 Rh6CG201000 Rh6CG235000 Rh6CG276000 Rh6CG420300 Rh6CG420400 Rh6DG498900 Rh7AG178800 Rh7AG275200 Rh7AG328700 Rh7AG328800 Rh7AG328900 Rh7AG353700 Rh7AG353800 Rh7AG367500 Rh7AG367600 Rh7AG394900 Rh7BG178500 Rh7BG321900 Rh7CG048800 Rh7CG346100 Rh7CG346200 Rh7CG346300 Rh7CG371300 Rh7CG385900 Rh7CG425400 Rh7DG106500 Rh7DG106600 Rh7DG352900 Rh7DG353000 Rh7DG361500 Rh7DG361600 Rh7DG362400 Rh7DG362500 Rh7DG363300
rosa_wichuraiana Rw0G002270 Rw0G002760 Rw0G019200 Rw1G000250 Rw1G008950 Rw1G010390 Rw1G012150 Rw1G039080 Rw2G001980 Rw2G003180 Rw2G003190 Rw4G008560 Rw4G010220 Rw4G012150 Rw5G005520 Rw5G009100 Rw5G024930 Rw5G035130 Rw5G047260 Rw5G048530 Rw6G007560 Rw6G016630 Rw6G041510 Rw7G030450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1576
AccB7I CCANNNNNTGG 1 cut(s) 392
AccI GTMKAC 3 cut(s) 115, 815, 2062
AciI CCGC 2 cut(s) 854, 1570
AclI AACGTT 1 cut(s) 472
AclWI GGATC 4 cut(s) 144, 332, 2133, 2199
AcoI YGGCCR 1 cut(s) 724
AcsI RAATTY 4 cut(s) 731, 832, 1426, 1635
AcuI CTGAAG 1 cut(s) 217
AcyI GRCGYC 1 cut(s) 807
AdeI CACNNNGTG 1 cut(s) 1036
AfaI GTAC 8 cut(s) 80, 270, 685, 1044, 1334, 1396, 1909, 2244
AfiI CCNNNNNNNGG 7 cut(s) 392, 583, 891, 1092, 1125, 1556, 1974
AflII CTTAAG 1 cut(s) 665
AflIII ACRYGT 1 cut(s) 2133
AgeI ACCGGT 1 cut(s) 403
AgsI TTSAA 8 cut(s) 124, 303, 838, 1307, 1496, 1531, 1580, 2468
AhdI GACNNNNNGTC 1 cut(s) 811
AhlI ACTAGT 1 cut(s) 1931
AjnI CCWGG 3 cut(s) 2159, 2197, 2230
AleI CACNNNNGTG 1 cut(s) 2561
AloI GAACNNNNNNTCC 2 cut(s) 2292, 2324
AluBI AGCT 7 cut(s) 719, 1361, 1963, 2264, 2328, 2343, 2384
AluI AGCT 7 cut(s) 719, 1361, 1963, 2264, 2328, 2343, 2384
Alw21I GWGCWC 4 cut(s) 1999, 2257, 2320, 2530
Alw26I GTCTC 2 cut(s) 679, 2312
Alw44I GTGCAC 1 cut(s) 2253
AlwI GGATC 4 cut(s) 144, 332, 2133, 2199
AlwNI CAGNNNCTG 1 cut(s) 2093
AoxI GGCC 4 cut(s) 724, 889, 1104, 2228
ApaLI GTGCAC 1 cut(s) 2253
ApeKI GCWGC 8 cut(s) 180, 517, 1325, 1560, 1821, 1960, 2223, 2264
ApoI RAATTY 4 cut(s) 731, 832, 1426, 1635
ArsI GACNNNNNNTTYG 6 cut(s) 367, 399, 1760, 1792, 2428, 2460
AseI ATTAAT 1 cut(s) 966
AsiGI ACCGGT 1 cut(s) 403
Asp700I GAANNNNTTC 2 cut(s) 1376, 2558
AspLEI GCGC 2 cut(s) 371, 522
AspS9I GGNCC 2 cut(s) 709, 2378
AsuC2I CCSGG 1 cut(s) 1550
AsuHPI GGTGA 9 cut(s) 292, 323, 503, 509, 815, 1048, 1300, 1784, 2000
AsuNHI GCTAGC 2 cut(s) 2260, 2339
AvaII GGWCC 2 cut(s) 709, 2378
BaeGI GKGCMC 2 cut(s) 2153, 2257
BaeI ACNNNNGTAYC 2 cut(s) 2362, 2395
BalI TGGCCA 1 cut(s) 726
BarI GAAGNNNNNNTAC 2 cut(s) 1683, 1715
BbsI GAAGAC 3 cut(s) 292, 589, 1850
Bbv12I GWGCWC 4 cut(s) 1999, 2257, 2320, 2530
BbvI GCAGC 8 cut(s) 192, 529, 1312, 1572, 1808, 1972, 2235, 2251
BccI CCATC 8 cut(s) 488, 716, 763, 794, 1802, 1978, 2093, 2539
BceAI ACGGC 3 cut(s) 992, 1009, 1119
BciT130I CCWGG 3 cut(s) 2161, 2199, 2232
BcnI CCSGG 1 cut(s) 1550
BcoDI GTCTC 2 cut(s) 679, 2312
BcuI ACTAGT 1 cut(s) 1931
BfaI CTAG 7 cut(s) 342, 543, 1932, 2261, 2340, 2439, 2475
BfmI CTRYAG 2 cut(s) 51, 927
BfoI RGCGCY 1 cut(s) 523
BfrI CTTAAG 1 cut(s) 665
BfuAI ACCTGC 1 cut(s) 1576
BglII AGATCT 1 cut(s) 142
BisI GCNGC 8 cut(s) 181, 518, 1326, 1561, 1822, 1961, 2224, 2265
BlsI GCNGC 8 cut(s) 182, 519, 1327, 1562, 1823, 1962, 2225, 2266
BmcAI AGTACT 1 cut(s) 1044
Bme1390I CCNGG 4 cut(s) 1550, 2161, 2199, 2232
Bme18I GGWCC 2 cut(s) 709, 2378
BmeRI GACNNNNNGTC 1 cut(s) 811
BmgT120I GGNCC 2 cut(s) 709, 2378
BmiI GGNNCC 3 cut(s) 1373, 2362, 2379
BmrFI CCNGG 4 cut(s) 1550, 2161, 2199, 2232
BmrI ACTGGG 1 cut(s) 805
BmsI GCATC 9 cut(s) 76, 439, 569, 851, 1195, 1397, 1509, 2326, 2467
BmtI GCTAGC 2 cut(s) 2264, 2343
BmuI ACTGGG 1 cut(s) 805
BpiI GAAGAC 3 cut(s) 292, 589, 1850
BpuEI CTTGAG 1 cut(s) 2542
BpuMI CCSGG 1 cut(s) 1550
BsaBI GATNNNNATC 1 cut(s) 102
BsaHI GRCGYC 1 cut(s) 807
BsaJI CCNNGG 4 cut(s) 40, 225, 1950, 2145
BsaWI WCCGGW 1 cut(s) 403
BsaXI ACNNNNNCTCC 4 cut(s) 2197, 2227, 2292, 2322
Bsc4I CCNNNNNNNGG 7 cut(s) 392, 583, 891, 1092, 1125, 1556, 1974
Bse118I RCCGGY 2 cut(s) 403, 891
Bse1I ACTGG 3 cut(s) 811, 2172, 2500
Bse3DI GCAATG 2 cut(s) 1335, 1422
Bse8I GATNNNNATC 1 cut(s) 102
BseBI CCWGG 3 cut(s) 2161, 2199, 2232
BseDI CCNNGG 4 cut(s) 40, 225, 1950, 2145
BseGI GGATG 6 cut(s) 67, 103, 958, 1210, 2302, 2422
BseJI GATNNNNATC 1 cut(s) 102
BseLI CCNNNNNNNGG 7 cut(s) 392, 583, 891, 1092, 1125, 1556, 1974
BseMI GCAATG 2 cut(s) 1335, 1422
BseMII CTCAG 1 cut(s) 2037
BseNI ACTGG 3 cut(s) 811, 2172, 2500
BseRI GAGGAG 1 cut(s) 1610
BseSI GKGCMC 2 cut(s) 2153, 2257
BseXI GCAGC 8 cut(s) 192, 529, 1312, 1572, 1808, 1972, 2235, 2251
BseYI CCCAGC 1 cut(s) 895
BsgI GTGCAG 1 cut(s) 2250
BshFI GGCC 4 cut(s) 726, 891, 1106, 2230
BshTI ACCGGT 1 cut(s) 403
BsiHKAI GWGCWC 4 cut(s) 1999, 2257, 2320, 2530
BsiSI CCGG 4 cut(s) 404, 892, 1245, 1550
BslFI GGGAC 2 cut(s) 409, 1172
BslI CCNNNNNNNGG 7 cut(s) 392, 583, 891, 1092, 1125, 1556, 1974
BsmAI GTCTC 2 cut(s) 679, 2312
BsmFI GGGAC 2 cut(s) 409, 1172
BsmI GAATGC 1 cut(s) 2164
BsnI GGCC 4 cut(s) 726, 891, 1106, 2230
Bsp1286I GDGCHC 5 cut(s) 1999, 2153, 2257, 2320, 2530
Bsp1407I TGTACA 2 cut(s) 1332, 1907
Bsp143I GATC 7 cut(s) 142, 149, 337, 1630, 1764, 2125, 2191
Bsp19I CCATGG 2 cut(s) 40, 225
BspACI CCGC 2 cut(s) 854, 1570
BspANI GGCC 4 cut(s) 726, 891, 1106, 2230
BspCNI CTCAG 1 cut(s) 2036
BspHI TCATGA 2 cut(s) 828, 1839
BspLI GGNNCC 3 cut(s) 1373, 2362, 2379
BspMI ACCTGC 1 cut(s) 1576
BspOI GCTAGC 2 cut(s) 2264, 2343
BspPI GGATC 4 cut(s) 144, 332, 2133, 2199
BspTI CTTAAG 1 cut(s) 665
BsrDI GCAATG 2 cut(s) 1335, 1422
BsrFI RCCGGY 2 cut(s) 403, 891
BsrGI TGTACA 2 cut(s) 1332, 1907
BsrI ACTGG 3 cut(s) 811, 2172, 2500
BssAI RCCGGY 2 cut(s) 403, 891
BssECI CCNNGG 4 cut(s) 40, 225, 1950, 2145
BssMI GATC 7 cut(s) 142, 149, 337, 1630, 1764, 2125, 2191
BssNI GRCGYC 1 cut(s) 807
BssT1I CCWWGG 4 cut(s) 40, 225, 1950, 2145
Bst2UI CCWGG 3 cut(s) 2161, 2199, 2232
Bst4CI ACNGT 8 cut(s) 291, 424, 1042, 1670, 1721, 1837, 2054, 2095
BstACI GRCGYC 1 cut(s) 807
BstAFI CTTAAG 1 cut(s) 665
BstAUI TGTACA 2 cut(s) 1332, 1907
BstC8I GCNNGC 7 cut(s) 17, 574, 893, 1965, 2228, 2262, 2341
BstDEI CTNAG 4 cut(s) 146, 380, 2023, 2057
BstDSI CCRYGG 2 cut(s) 40, 225
BstF5I GGATG 6 cut(s) 67, 103, 958, 1210, 2302, 2422
BstH2I RGCGCY 1 cut(s) 523
BstHHI GCGC 2 cut(s) 371, 522
BstKTI GATC 7 cut(s) 145, 152, 340, 1633, 1767, 2128, 2194
BstMAI GTCTC 2 cut(s) 679, 2312
BstMBI GATC 7 cut(s) 142, 149, 337, 1630, 1764, 2125, 2191
BstMWI GCNNNNNNNGC 3 cut(s) 1064, 2261, 2534
BstNI CCWGG 3 cut(s) 2161, 2199, 2232
BstNSI RCATGY 4 cut(s) 19, 690, 1570, 1745
BstSCI CCNGG 4 cut(s) 1548, 2159, 2197, 2230
BstSFI CTRYAG 2 cut(s) 51, 927
BstSLI GKGCMC 2 cut(s) 2153, 2257
BstV1I GCAGC 8 cut(s) 192, 529, 1312, 1572, 1808, 1972, 2235, 2251
BstV2I GAAGAC 3 cut(s) 292, 589, 1850
BstX2I RGATCY 3 cut(s) 142, 149, 2125
BstXI CCANNNNNNTGG 1 cut(s) 722
BstYI RGATCY 3 cut(s) 142, 149, 2125
BsuRI GGCC 4 cut(s) 726, 891, 1106, 2230
BtgI CCRYGG 2 cut(s) 40, 225
BtsCI GGATG 6 cut(s) 67, 103, 958, 1210, 2302, 2422
BtsI GCAGTG 2 cut(s) 198, 2255
BtsIMutI CAGTG 6 cut(s) 31, 198, 880, 1149, 2179, 2255
BveI ACCTGC 1 cut(s) 1576
Cac8I GCNNGC 7 cut(s) 17, 574, 893, 1965, 2228, 2262, 2341
CaiI CAGNNNCTG 1 cut(s) 2093
CciI TCATGA 2 cut(s) 828, 1839
CfoI GCGC 2 cut(s) 371, 522
Cfr10I RCCGGY 2 cut(s) 403, 891
Cfr13I GGNCC 2 cut(s) 709, 2378
CseI GACGC 2 cut(s) 172, 815
Csp6I GTAC 8 cut(s) 79, 269, 684, 1043, 1333, 1395, 1908, 2243
CspAI ACCGGT 1 cut(s) 403
CspCI CAANNNNNGTGG 2 cut(s) 2158, 2193
CviQI GTAC 8 cut(s) 79, 269, 684, 1043, 1333, 1395, 1908, 2243
DdeI CTNAG 4 cut(s) 146, 380, 2023, 2057
DpnI GATC 7 cut(s) 144, 151, 339, 1632, 1766, 2127, 2193
DpnII GATC 7 cut(s) 142, 149, 337, 1630, 1764, 2125, 2191
DraI TTTAAA 1 cut(s) 1431
DraIII CACNNNGTG 1 cut(s) 1036
DriI GACNNNNNGTC 1 cut(s) 811
EaeI YGGCCR 1 cut(s) 724
Eam1105I GACNNNNNGTC 1 cut(s) 811
EciI GGCGGA 1 cut(s) 869
Eco130I CCWWGG 4 cut(s) 40, 225, 1950, 2145
Eco47I GGWCC 2 cut(s) 709, 2378
Eco57I CTGAAG 1 cut(s) 217
EcoRII CCWGG 3 cut(s) 2159, 2197, 2230
EcoT14I CCWWGG 4 cut(s) 40, 225, 1950, 2145
EcoT22I ATGCAT 1 cut(s) 1422
ErhI CCWWGG 4 cut(s) 40, 225, 1950, 2145
FaqI GGGAC 2 cut(s) 409, 1172
FauNDI CATATG 2 cut(s) 2035, 2451
FblI GTMKAC 3 cut(s) 115, 815, 2062
Fnu4HI GCNGC 8 cut(s) 181, 518, 1326, 1561, 1822, 1961, 2224, 2265
FokI GGATG 6 cut(s) 74, 110, 965, 1217, 2309, 2429
Fsp4HI GCNGC 8 cut(s) 181, 518, 1326, 1561, 1822, 1961, 2224, 2265
FspBI CTAG 7 cut(s) 342, 543, 1932, 2261, 2340, 2439, 2475
GlaI GCGC 2 cut(s) 370, 521
GluI GCNGC 8 cut(s) 181, 518, 1326, 1561, 1822, 1961, 2224, 2265
GsaI CCCAGC 1 cut(s) 899
HaeII RGCGCY 1 cut(s) 523
HaeIII GGCC 4 cut(s) 726, 891, 1106, 2230
HapII CCGG 4 cut(s) 404, 892, 1245, 1550
HgaI GACGC 2 cut(s) 172, 815
HhaI GCGC 2 cut(s) 371, 522
Hin1I GRCGYC 1 cut(s) 807
Hin6I GCGC 2 cut(s) 369, 520
HinP1I GCGC 2 cut(s) 369, 520
HincII GTYRAC 3 cut(s) 427, 2063, 2447
HindII GTYRAC 3 cut(s) 427, 2063, 2447
HindIII AAGCTT 1 cut(s) 1359
HpaI GTTAAC 1 cut(s) 427
HpaII CCGG 4 cut(s) 404, 892, 1245, 1550
HphI GGTGA 9 cut(s) 292, 323, 503, 509, 815, 1048, 1300, 1784, 2000
Hpy188I TCNGA 9 cut(s) 73, 157, 242, 778, 1253, 1807, 2026, 2130, 2406
Hpy188III TCNNGA 6 cut(s) 107, 829, 1307, 1840, 1868, 2501
HpyAV CCTTC 4 cut(s) 778, 881, 1874, 2287
HpyCH4III ACNGT 8 cut(s) 291, 424, 1042, 1670, 1721, 1837, 2054, 2095
HpyCH4IV ACGT 4 cut(s) 472, 1611, 2133, 2480
HpyF10VI GCNNNNNNNGC 3 cut(s) 1064, 2261, 2534
HpyF3I CTNAG 4 cut(s) 146, 380, 2023, 2057
HpySE526I ACGT 4 cut(s) 472, 1611, 2133, 2480
Hsp92I GRCGYC 1 cut(s) 807
HspAI GCGC 2 cut(s) 369, 520
KroI GCCGGC 1 cut(s) 891
KroNI GCCGGC 1 cut(s) 893
KspAI GTTAAC 1 cut(s) 427
Kzo9I GATC 7 cut(s) 142, 149, 337, 1630, 1764, 2125, 2191
LmnI GCTCC 2 cut(s) 2333, 2533
Lsp1109I GCAGC 8 cut(s) 192, 529, 1312, 1572, 1808, 1972, 2235, 2251
LweI GCATC 9 cut(s) 76, 439, 569, 851, 1195, 1397, 1509, 2326, 2467
MaeI CTAG 7 cut(s) 342, 543, 1932, 2261, 2340, 2439, 2475
MaeII ACGT 4 cut(s) 472, 1611, 2133, 2480
MalI GATC 7 cut(s) 144, 151, 339, 1632, 1766, 2127, 2193
MboI GATC 7 cut(s) 142, 149, 337, 1630, 1764, 2125, 2191
MfeI CAATTG 2 cut(s) 1090, 1491
MflI RGATCY 3 cut(s) 142, 149, 2125
MhlI GDGCHC 5 cut(s) 1999, 2153, 2257, 2320, 2530
MlsI TGGCCA 1 cut(s) 726
MluNI TGGCCA 1 cut(s) 726
MlyI GAGTC 5 cut(s) 767, 1242, 1655, 1811, 2357
Mox20I TGGCCA 1 cut(s) 726
Mph1103I ATGCAT 1 cut(s) 1422
MroNI GCCGGC 1 cut(s) 891
MroXI GAANNNNTTC 2 cut(s) 1376, 2558
MscI TGGCCA 1 cut(s) 726
MslI CAYNNNNRTG 5 cut(s) 123, 577, 1838, 2112, 2561
Msp20I TGGCCA 1 cut(s) 726
MspA1I CMGCKG 1 cut(s) 2384
MspCI CTTAAG 1 cut(s) 665
MspI CCGG 4 cut(s) 404, 892, 1245, 1550
MspR9I CCNGG 4 cut(s) 1550, 2161, 2199, 2232
MunI CAATTG 2 cut(s) 1090, 1491
Mva1269I GAATGC 1 cut(s) 2164
MvaI CCWGG 3 cut(s) 2161, 2199, 2232
MwoI GCNNNNNNNGC 3 cut(s) 1064, 2261, 2534
NaeI GCCGGC 1 cut(s) 893
NciI CCSGG 1 cut(s) 1550
NcoI CCATGG 2 cut(s) 40, 225
NdeI CATATG 2 cut(s) 2035, 2451
NdeII GATC 7 cut(s) 142, 149, 337, 1630, 1764, 2125, 2191
NgoMIV GCCGGC 1 cut(s) 891
NheI GCTAGC 2 cut(s) 2260, 2339
NlaIV GGNNCC 3 cut(s) 1373, 2362, 2379
NmuCI GTSAC 6 cut(s) 803, 1036, 1288, 1744, 1790, 2498
NsiI ATGCAT 1 cut(s) 1422
NspI RCATGY 4 cut(s) 19, 690, 1570, 1745
OliI CACNNNNGTG 1 cut(s) 2561
PaeI GCATGC 1 cut(s) 19
PagI TCATGA 2 cut(s) 828, 1839
PctI GAATGC 1 cut(s) 2164
PdiI GCCGGC 1 cut(s) 893
PdmI GAANNNNTTC 2 cut(s) 1376, 2558
PfeI GAWTC 5 cut(s) 21, 103, 1684, 1871, 2471
PflMI CCANNNNNTGG 1 cut(s) 392
PinAI ACCGGT 1 cut(s) 403
PkrI GCNGC 8 cut(s) 182, 519, 1327, 1562, 1823, 1962, 2225, 2266
PleI GAGTC 5 cut(s) 767, 1242, 1654, 1810, 2356
PpsI GAGTC 5 cut(s) 767, 1242, 1654, 1810, 2356
PshBI ATTAAT 1 cut(s) 966
Psp1406I AACGTT 1 cut(s) 472
Psp6I CCWGG 3 cut(s) 2159, 2197, 2230
PspFI CCCAGC 1 cut(s) 895
PspGI CCWGG 3 cut(s) 2159, 2197, 2230
PspN4I GGNNCC 3 cut(s) 1373, 2362, 2379
PspPI GGNCC 2 cut(s) 709, 2378
PsrI GAACNNNNNNTAC 2 cut(s) 457, 489
PstNI CAGNNNCTG 1 cut(s) 2093
PsuI RGATCY 3 cut(s) 142, 149, 2125
PvuII CAGCTG 1 cut(s) 2384
RsaI GTAC 8 cut(s) 80, 270, 685, 1044, 1334, 1396, 1909, 2244
RsaNI GTAC 8 cut(s) 79, 269, 684, 1043, 1333, 1395, 1908, 2243
RseI CAYNNNNRTG 5 cut(s) 123, 577, 1838, 2112, 2561
SalI GTCGAC 1 cut(s) 2061
SatI GCNGC 8 cut(s) 181, 518, 1326, 1561, 1822, 1961, 2224, 2265
Sau3AI GATC 7 cut(s) 142, 149, 337, 1630, 1764, 2125, 2191
Sau96I GGNCC 2 cut(s) 709, 2378
ScaI AGTACT 1 cut(s) 1044
SchI GAGTC 5 cut(s) 767, 1242, 1655, 1811, 2357
ScrFI CCNGG 4 cut(s) 1550, 2161, 2199, 2232
SduI GDGCHC 5 cut(s) 1999, 2153, 2257, 2320, 2530
SfaNI GCATC 9 cut(s) 76, 439, 569, 851, 1195, 1397, 1509, 2326, 2467
SfcI CTRYAG 2 cut(s) 51, 927
SinI GGWCC 2 cut(s) 709, 2378
SmiMI CAYNNNNRTG 5 cut(s) 123, 577, 1838, 2112, 2561
SmlI CTYRAG 2 cut(s) 665, 2521
SmoI CTYRAG 2 cut(s) 665, 2521
SpeI ACTAGT 1 cut(s) 1931
SphI GCATGC 1 cut(s) 19
SsiI CCGC 2 cut(s) 854, 1570
SspI AATATT 1 cut(s) 538
SspMI CTAG 7 cut(s) 342, 543, 1932, 2261, 2340, 2439, 2475
StyD4I CCNGG 4 cut(s) 1548, 2159, 2197, 2230
StyI CCWWGG 4 cut(s) 40, 225, 1950, 2145
TaaI ACNGT 8 cut(s) 291, 424, 1042, 1670, 1721, 1837, 2054, 2095
TaiI ACGT 4 cut(s) 475, 1614, 2136, 2483
TaqI TCGA 2 cut(s) 1767, 2062
TatI WGTACW 4 cut(s) 268, 1042, 1332, 1907
TfiI GAWTC 5 cut(s) 21, 103, 1684, 1871, 2471
TscAI CASTG 6 cut(s) 31, 198, 880, 1156, 2179, 2262
TseFI GTSAC 6 cut(s) 803, 1036, 1288, 1744, 1790, 2498
TseI GCWGC 8 cut(s) 180, 517, 1325, 1560, 1821, 1960, 2223, 2264
Tsp45I GTSAC 6 cut(s) 803, 1036, 1288, 1744, 1790, 2498
TspGWI ACGGA 2 cut(s) 590, 2335
TspRI CASTG 6 cut(s) 31, 198, 880, 1156, 2179, 2262
Van91I CCANNNNNTGG 1 cut(s) 392
Vha464I CTTAAG 1 cut(s) 665
VneI GTGCAC 1 cut(s) 2253
VpaK11BI GGWCC 2 cut(s) 709, 2378
VspI ATTAAT 1 cut(s) 966
XapI RAATTY 4 cut(s) 731, 832, 1426, 1635
XceI RCATGY 4 cut(s) 19, 690, 1570, 1745
XcmI CCANNNNNNNNNTGG 3 cut(s) 719, 734, 1382
XmiI GTMKAC 3 cut(s) 115, 815, 2062
XmnI GAANNNNTTC 2 cut(s) 1376, 2558
XspI CTAG 7 cut(s) 342, 543, 1932, 2261, 2340, 2439, 2475
ZrmI AGTACT 1 cut(s) 1044
Zsp2I ATGCAT 1 cut(s) 1422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.