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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 62 |
| AccII | CGCG | 2 cut(s) | 344, 346 |
| AciI | CCGC | 2 cut(s) | 138, 342 |
| AcsI | RAATTY | 1 cut(s) | 461 |
| AcuI | CTGAAG | 1 cut(s) | 36 |
| AfaI | GTAC | 1 cut(s) | 123 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 19, 63 |
| AflII | CTTAAG | 1 cut(s) | 149 |
| AgeI | ACCGGT | 1 cut(s) | 376 |
| AgsI | TTSAA | 4 cut(s) | 105, 351, 383, 452 |
| AjnI | CCWGG | 1 cut(s) | 406 |
| ApeKI | GCWGC | 1 cut(s) | 482 |
| ApoI | RAATTY | 1 cut(s) | 461 |
| AsiGI | ACCGGT | 1 cut(s) | 376 |
| AspA2I | CCTAGG | 1 cut(s) | 13 |
| AspLEI | GCGC | 2 cut(s) | 181, 346 |
| AsuHPI | GGTGA | 1 cut(s) | 396 |
| AvrII | CCTAGG | 1 cut(s) | 13 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 417, 449 |
| BbvI | GCAGC | 1 cut(s) | 469 |
| BccI | CCATC | 2 cut(s) | 182, 286 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 164, 198 |
| BciT130I | CCWGG | 1 cut(s) | 408 |
| BfaI | CTAG | 2 cut(s) | 14, 119 |
| BfmI | CTRYAG | 2 cut(s) | 156, 247 |
| BfrI | CTTAAG | 1 cut(s) | 149 |
| BfuAI | ACCTGC | 1 cut(s) | 62 |
| BglII | AGATCT | 1 cut(s) | 160 |
| BisI | GCNGC | 1 cut(s) | 483 |
| BlnI | CCTAGG | 1 cut(s) | 13 |
| BlsI | GCNGC | 1 cut(s) | 484 |
| Bme1390I | CCNGG | 1 cut(s) | 408 |
| BmiI | GGNNCC | 1 cut(s) | 193 |
| BmrFI | CCNGG | 1 cut(s) | 408 |
| BmsI | GCATC | 1 cut(s) | 166 |
| BsaBI | GATNNNNATC | 1 cut(s) | 277 |
| BsaJI | CCNNGG | 1 cut(s) | 13 |
| BsaWI | WCCGGW | 1 cut(s) | 376 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 19, 63 |
| Bse118I | RCCGGY | 1 cut(s) | 376 |
| Bse8I | GATNNNNATC | 1 cut(s) | 277 |
| BseBI | CCWGG | 1 cut(s) | 408 |
| BseDI | CCNNGG | 1 cut(s) | 13 |
| BseJI | GATNNNNATC | 1 cut(s) | 277 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 19, 63 |
| BseMII | CTCAG | 1 cut(s) | 386 |
| BseXI | GCAGC | 1 cut(s) | 469 |
| Bsh1236I | CGCG | 2 cut(s) | 344, 346 |
| BshTI | ACCGGT | 1 cut(s) | 376 |
| BsiSI | CCGG | 1 cut(s) | 377 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 19, 63 |
| Bsp143I | GATC | 1 cut(s) | 160 |
| BspACI | CCGC | 2 cut(s) | 138, 342 |
| BspCNI | CTCAG | 1 cut(s) | 387 |
| BspFNI | CGCG | 2 cut(s) | 344, 346 |
| BspHI | TCATGA | 1 cut(s) | 265 |
| BspLI | GGNNCC | 1 cut(s) | 193 |
| BspMI | ACCTGC | 1 cut(s) | 62 |
| BspTI | CTTAAG | 1 cut(s) | 149 |
| BsrFI | RCCGGY | 1 cut(s) | 376 |
| BssAI | RCCGGY | 1 cut(s) | 376 |
| BssECI | CCNNGG | 1 cut(s) | 13 |
| BssMI | GATC | 1 cut(s) | 160 |
| BssT1I | CCWWGG | 1 cut(s) | 13 |
| Bst2UI | CCWGG | 1 cut(s) | 408 |
| Bst4CI | ACNGT | 1 cut(s) | 112 |
| BstAFI | CTTAAG | 1 cut(s) | 149 |
| BstC8I | GCNNGC | 1 cut(s) | 183 |
| BstDEI | CTNAG | 1 cut(s) | 395 |
| BstEII | GGTNACC | 1 cut(s) | 337 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 17 |
| BstFNI | CGCG | 2 cut(s) | 344, 346 |
| BstHHI | GCGC | 2 cut(s) | 181, 346 |
| BstKTI | GATC | 1 cut(s) | 163 |
| BstMBI | GATC | 1 cut(s) | 160 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 32, 191 |
| BstNI | CCWGG | 1 cut(s) | 408 |
| BstPI | GGTNACC | 1 cut(s) | 337 |
| BstSCI | CCNGG | 1 cut(s) | 406 |
| BstSFI | CTRYAG | 2 cut(s) | 156, 247 |
| BstUI | CGCG | 2 cut(s) | 344, 346 |
| BstV1I | GCAGC | 1 cut(s) | 469 |
| BstX2I | RGATCY | 1 cut(s) | 160 |
| BstYI | RGATCY | 1 cut(s) | 160 |
| BveI | ACCTGC | 1 cut(s) | 62 |
| Cac8I | GCNNGC | 1 cut(s) | 183 |
| CciI | TCATGA | 1 cut(s) | 265 |
| CfoI | GCGC | 2 cut(s) | 181, 346 |
| Cfr10I | RCCGGY | 1 cut(s) | 376 |
| Csp6I | GTAC | 1 cut(s) | 122 |
| CspAI | ACCGGT | 1 cut(s) | 376 |
| CviAII | CATG | 3 cut(s) | 220, 266, 437 |
| CviJI | RGCY | 4 cut(s) | 35, 56, 155, 194 |
| CviKI_1 | RGCY | 4 cut(s) | 35, 56, 155, 194 |
| CviQI | GTAC | 1 cut(s) | 122 |
| DdeI | CTNAG | 1 cut(s) | 395 |
| DpnI | GATC | 1 cut(s) | 162 |
| DpnII | GATC | 1 cut(s) | 160 |
| Eco130I | CCWWGG | 1 cut(s) | 13 |
| Eco57I | CTGAAG | 1 cut(s) | 36 |
| Eco91I | GGTNACC | 1 cut(s) | 337 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 17 |
| EcoO65I | GGTNACC | 1 cut(s) | 337 |
| EcoRII | CCWGG | 1 cut(s) | 406 |
| EcoT14I | CCWWGG | 1 cut(s) | 13 |
| ErhI | CCWWGG | 1 cut(s) | 13 |
| FaeI | CATG | 3 cut(s) | 223, 269, 440 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 225, 257 |
| FatI | CATG | 3 cut(s) | 219, 265, 436 |
| FauNDI | CATATG | 1 cut(s) | 131 |
| Fnu4HI | GCNGC | 1 cut(s) | 483 |
| Fsp4HI | GCNGC | 1 cut(s) | 483 |
| FspBI | CTAG | 2 cut(s) | 14, 119 |
| GlaI | GCGC | 2 cut(s) | 180, 345 |
| GluI | GCNGC | 1 cut(s) | 483 |
| HapII | CCGG | 1 cut(s) | 377 |
| HhaI | GCGC | 2 cut(s) | 181, 346 |
| Hin1II | CATG | 3 cut(s) | 223, 269, 440 |
| Hin6I | GCGC | 2 cut(s) | 179, 344 |
| HinP1I | GCGC | 2 cut(s) | 179, 344 |
| HinfI | GANTC | 2 cut(s) | 293, 383 |
| HpaII | CCGG | 1 cut(s) | 377 |
| HphI | GGTGA | 1 cut(s) | 396 |
| Hpy166II | GTNNAC | 1 cut(s) | 77 |
| Hpy188I | TCNGA | 2 cut(s) | 283, 298 |
| Hpy188III | TCNNGA | 1 cut(s) | 266 |
| Hpy8I | GTNNAC | 1 cut(s) | 77 |
| HpyAV | CCTTC | 3 cut(s) | 13, 67, 227 |
| HpyCH4III | ACNGT | 1 cut(s) | 112 |
| HpyCH4IV | ACGT | 1 cut(s) | 124 |
| HpyCH4V | TGCA | 3 cut(s) | 135, 185, 485 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 32, 191 |
| HpyF3I | CTNAG | 1 cut(s) | 395 |
| HpySE526I | ACGT | 1 cut(s) | 124 |
| Hsp92II | CATG | 3 cut(s) | 223, 269, 440 |
| HspAI | GCGC | 2 cut(s) | 179, 344 |
| Kzo9I | GATC | 1 cut(s) | 160 |
| LmnI | GCTCC | 1 cut(s) | 191 |
| LpnPI | CCDG | 5 cut(s) | 57, 390, 393, 420, 471 |
| Lsp1109I | GCAGC | 1 cut(s) | 469 |
| LweI | GCATC | 1 cut(s) | 166 |
| MaeI | CTAG | 2 cut(s) | 14, 119 |
| MaeII | ACGT | 1 cut(s) | 124 |
| MaeIII | GTNAC | 1 cut(s) | 337 |
| MalI | GATC | 1 cut(s) | 162 |
| MboI | GATC | 1 cut(s) | 160 |
| MboII | GAAGA | 3 cut(s) | 299, 311, 437 |
| MflI | RGATCY | 1 cut(s) | 160 |
| MluCI | AATT | 2 cut(s) | 312, 461 |
| MnlI | CCTC | 4 cut(s) | 328, 349, 390, 415 |
| MseI | TTAA | 2 cut(s) | 150, 468 |
| MslI | CAYNNNNRTG | 1 cut(s) | 477 |
| MspCI | CTTAAG | 1 cut(s) | 149 |
| MspI | CCGG | 1 cut(s) | 377 |
| MspR9I | CCNGG | 1 cut(s) | 408 |
| MvaI | CCWGG | 1 cut(s) | 408 |
| MvnI | CGCG | 2 cut(s) | 344, 346 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 32, 191 |
| NdeI | CATATG | 1 cut(s) | 131 |
| NdeII | GATC | 1 cut(s) | 160 |
| NlaIII | CATG | 3 cut(s) | 223, 269, 440 |
| NlaIV | GGNNCC | 1 cut(s) | 193 |
| PagI | TCATGA | 1 cut(s) | 265 |
| PfeI | GAWTC | 2 cut(s) | 293, 383 |
| PinAI | ACCGGT | 1 cut(s) | 376 |
| PkrI | GCNGC | 1 cut(s) | 484 |
| Psp6I | CCWGG | 1 cut(s) | 406 |
| PspEI | GGTNACC | 1 cut(s) | 337 |
| PspGI | CCWGG | 1 cut(s) | 406 |
| PspN4I | GGNNCC | 1 cut(s) | 193 |
| PsuI | RGATCY | 1 cut(s) | 160 |
| RsaI | GTAC | 1 cut(s) | 123 |
| RsaNI | GTAC | 1 cut(s) | 122 |
| RseI | CAYNNNNRTG | 1 cut(s) | 477 |
| SaqAI | TTAA | 2 cut(s) | 150, 468 |
| SatI | GCNGC | 1 cut(s) | 483 |
| Sau3AI | GATC | 1 cut(s) | 160 |
| ScrFI | CCNGG | 1 cut(s) | 408 |
| SetI | ASST | 7 cut(s) | 24, 76, 127, 238, 339, 409, 460 |
| SfaNI | GCATC | 1 cut(s) | 166 |
| SfcI | CTRYAG | 2 cut(s) | 156, 247 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 477 |
| SmlI | CTYRAG | 1 cut(s) | 149 |
| SmoI | CTYRAG | 1 cut(s) | 149 |
| Sse9I | AATT | 2 cut(s) | 312, 461 |
| SsiI | CCGC | 2 cut(s) | 138, 342 |
| SspMI | CTAG | 2 cut(s) | 14, 119 |
| StyD4I | CCNGG | 1 cut(s) | 406 |
| StyI | CCWWGG | 1 cut(s) | 13 |
| TaaI | ACNGT | 1 cut(s) | 112 |
| TaiI | ACGT | 1 cut(s) | 127 |
| TaqI | TCGA | 2 cut(s) | 174, 316 |
| TasI | AATT | 2 cut(s) | 312, 461 |
| TfiI | GAWTC | 2 cut(s) | 293, 383 |
| Tru1I | TTAA | 2 cut(s) | 150, 468 |
| Tru9I | TTAA | 2 cut(s) | 150, 468 |
| TseI | GCWGC | 1 cut(s) | 482 |
| TspDTI | ATGAA | 5 cut(s) | 118, 246, 282, 306, 318 |
| Vha464I | CTTAAG | 1 cut(s) | 149 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 17 |
| XapI | RAATTY | 1 cut(s) | 461 |
| XmaJI | CCTAGG | 1 cut(s) | 13 |
| XspI | CTAG | 2 cut(s) | 14, 119 |