pycom581g00100

mitotic sister chromatid biorientation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_581
OG0000098
Physical Location & Seq
Forward (+)
184115 .. 185482
1368 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom581g00100.1

Sequence Viewer

Length: 933 bp
ATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATCTGCACCGACGGCCGCTCCGCCCGGGCTCGCGCCCCAGGTTTTGCGGCGACCGCCGCGCCCTCCTACTCATCGGGGCCTGGCGCTTGCCCCGACGGCCGGGTGTAGGTCGCGCGCTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGACGAACGATTTGCACGTCAGTATCGCTGCGGGCCTCCACCAGAGTTTCCTCTGGCTTCGCCCCGCTCAGGCATAGTTCACCATCTTTCGGGTCCCGACAGGCATGCTCTCACTCGAACCCTTCTCAGAAGATCAAGGTCGGTCGGCGGTGCAACCCTCGTGGGGATCCCGCCAGTCAGCTTCCTTGCGCCTTACGGGTTTGATCGCCCGTTGACTCGCACGCATGTCAGACTCCTTGGTCCGTGTTTCAAGACGGGCCGAATGGGGAGCTCGCTGGCCGACGCCGGGAGCGCGCAGTTGCCGAAGCACGCCGGTACGGCGCGCGCTGCCCTCCACGATCGCGTCGACGGCGTCTCCGCGGGCATATCAACAGCCCGGGCTTTGGCCGCCGCCGCAATCCGCGTCGGTCCACGCCCCGAGCCGATCGGCGGACCGGCTTTCGACCGTTCCACATCCGACCGGGGCGCATCGCCAGCCCCCATCCGCTTCCCTCCCGACAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTCCCTCGCGGTACTTGTTTGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACAGAATTTACCGCCCGATTGGGGCTGCATTCCCAAACAACCCGACTCGCCGACAGCGCCTCGTGGTGCGACAGGTGGACTTGGGCCCGGTCCGCCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

311

Amino Acids

33.97

Weight (kDa)

11.64

Isoelectric Point (pI)

52.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000098)

Species Orthologous Gene IDs
pyrus_communis pycom05g23460 pycom581g00100 pycom849g00040
rosa_multiflora Rmu_sc0019961.1_g000001
rosa_roxburghii Rroxscaffold_100G00451180 Rroxscaffold_102G00450890 Rroxscaffold_102G00450920 Rroxscaffold_103G00450530 Rroxscaffold_103G00450560 Rroxscaffold_104G00444830 Rroxscaffold_104G00444900 Rroxscaffold_105G00447100 Rroxscaffold_105G00447180 Rroxscaffold_107G00442470 Rroxscaffold_107G00442480 Rroxscaffold_107G00442520 Rroxscaffold_107G00442540 Rroxscaffold_107G00442560 Rroxscaffold_107G00442570 Rroxscaffold_107G00442600 Rroxscaffold_107G00442630 Rroxscaffold_107G00442650 Rroxscaffold_107G00442660 Rroxscaffold_108G00451480 Rroxscaffold_110G00451510 Rroxscaffold_111G00451550 Rroxscaffold_112G00451590 Rroxscaffold_113G00451630 Rroxscaffold_114G00451650 Rroxscaffold_116G00451710 Rroxscaffold_117G00451740 Rroxscaffold_117G00451750 Rroxscaffold_121G00451840 Rroxscaffold_122G00451860 Rroxscaffold_122G00451880 Rroxscaffold_123G00451920 Rroxscaffold_124G00451950 Rroxscaffold_128G00452040 Rroxscaffold_128G00452060 Rroxscaffold_12G00450600 Rroxscaffold_12G00450620 Rroxscaffold_131G00452110 Rroxscaffold_138G00452180 Rroxscaffold_13G00448630 Rroxscaffold_145G00452240 Rroxscaffold_14G00441930 Rroxscaffold_14G00441950 Rroxscaffold_14G00442050 Rroxscaffold_14G00442080 Rroxscaffold_15G00447500 Rroxscaffold_162G00450770 Rroxscaffold_16G00446200 Rroxscaffold_16G00446210 Rroxscaffold_16G00446240 Rroxscaffold_17G00435360 Rroxscaffold_17G00435410 Rroxscaffold_17G00435450 Rroxscaffold_17G00435480 Rroxscaffold_17G00435500 Rroxscaffold_17G00435600 Rroxscaffold_17G00435660 Rroxscaffold_17G00435680 Rroxscaffold_17G00435760 Rroxscaffold_17G00435780 Rroxscaffold_17G00435810 Rroxscaffold_17G00435840 Rroxscaffold_17G00435870 Rroxscaffold_18G00446030 Rroxscaffold_18G00446060 Rroxscaffold_18G00446130 Rroxscaffold_19G00448890 Rroxscaffold_19G00448920 Rroxscaffold_19G00448940 Rroxscaffold_19G00448970 Rroxscaffold_1G00000020 Rroxscaffold_1G00000050 Rroxscaffold_1G00000160 Rroxscaffold_20G00445060 Rroxscaffold_20G00445100 Rroxscaffold_20G00445130 Rroxscaffold_21G00439490 Rroxscaffold_21G00439560 Rroxscaffold_21G00439580 Rroxscaffold_21G00439620 Rroxscaffold_21G00439630 Rroxscaffold_22G00439910 Rroxscaffold_22G00439950 Rroxscaffold_23G00451020 Rroxscaffold_23G00451040 Rroxscaffold_23G00451070 Rroxscaffold_24G00444920 Rroxscaffold_24G00444950 Rroxscaffold_24G00445000 Rroxscaffold_25G00450200 Rroxscaffold_26G00447730 Rroxscaffold_27G00446570 Rroxscaffold_27G00446600 Rroxscaffold_28G00446830 Rroxscaffold_28G00446860 Rroxscaffold_28G00446910 Rroxscaffold_29G00441560 Rroxscaffold_29G00441570 Rroxscaffold_29G00441590 Rroxscaffold_29G00441630 Rroxscaffold_29G00441640 Rroxscaffold_29G00441670 Rroxscaffold_29G00441680 Rroxscaffold_29G00441860 Rroxscaffold_30G00449540 Rroxscaffold_30G00449570 Rroxscaffold_30G00449580 Rroxscaffold_31G00438120 Rroxscaffold_31G00438140 Rroxscaffold_31G00438180 Rroxscaffold_31G00438190 Rroxscaffold_31G00438220 Rroxscaffold_31G00438230 Rroxscaffold_32G00442740 Rroxscaffold_32G00442760 Rroxscaffold_33G00439670 Rroxscaffold_33G00439720 Rroxscaffold_33G00439740 Rroxscaffold_33G00439760 Rroxscaffold_33G00439820 Rroxscaffold_34G00443140 Rroxscaffold_34G00443200 Rroxscaffold_34G00443250 Rroxscaffold_34G00443370 Rroxscaffold_34G00443390 Rroxscaffold_35G00441050 Rroxscaffold_35G00441080 Rroxscaffold_35G00441090 Rroxscaffold_35G00441170 Rroxscaffold_35G00441190 Rroxscaffold_35G00441230 Rroxscaffold_36G00440100 Rroxscaffold_36G00440170 Rroxscaffold_37G00445170 Rroxscaffold_38G00444440 Rroxscaffold_38G00444500 Rroxscaffold_38G00444550 Rroxscaffold_39G00448200 Rroxscaffold_39G00448210 Rroxscaffold_40G00447650 Rroxscaffold_40G00447680 Rroxscaffold_40G00447700 Rroxscaffold_40G00447710 Rroxscaffold_41G00451110 Rroxscaffold_41G00451130 Rroxscaffold_42G00450430 Rroxscaffold_43G00449800 Rroxscaffold_43G00449860 Rroxscaffold_43G00449920 Rroxscaffold_43G00449930 Rroxscaffold_44G00440540 Rroxscaffold_44G00440580 Rroxscaffold_44G00440660 Rroxscaffold_45G00438900 Rroxscaffold_45G00438930 Rroxscaffold_45G00438950 Rroxscaffold_47G00444020 Rroxscaffold_47G00444060 Rroxscaffold_47G00444120 Rroxscaffold_48G00448450 Rroxscaffold_49G00438570 Rroxscaffold_49G00438650 Rroxscaffold_49G00438700 Rroxscaffold_4G00321080 Rroxscaffold_50G00444130 Rroxscaffold_50G00444200 Rroxscaffold_50G00444210 Rroxscaffold_50G00444330 Rroxscaffold_50G00444350 Rroxscaffold_51G00447840 Rroxscaffold_52G00439310 Rroxscaffold_52G00439390 Rroxscaffold_54G00443660 Rroxscaffold_55G00450020 Rroxscaffold_55G00450050 Rroxscaffold_55G00450060 Rroxscaffold_55G00450080 Rroxscaffold_55G00450090 Rroxscaffold_55G00450120 Rroxscaffold_57G00443070 Rroxscaffold_59G00442210 Rroxscaffold_59G00442220 Rroxscaffold_60G00448360 Rroxscaffold_60G00448370 Rroxscaffold_60G00448410 Rroxscaffold_61G00449990 Rroxscaffold_62G00438040 Rroxscaffold_62G00438060 Rroxscaffold_62G00438080 Rroxscaffold_63G00444410 Rroxscaffold_63G00444430 Rroxscaffold_64G00450670 Rroxscaffold_65G00445300 Rroxscaffold_65G00445340 Rroxscaffold_65G00445360 Rroxscaffold_65G00445380 Rroxscaffold_65G00445420 Rroxscaffold_66G00437660 Rroxscaffold_66G00437820 Rroxscaffold_66G00437840 Rroxscaffold_66G00437890 Rroxscaffold_67G00448140 Rroxscaffold_67G00448150 Rroxscaffold_68G00447010 Rroxscaffold_69G00446670 Rroxscaffold_69G00446710 Rroxscaffold_69G00446770 Rroxscaffold_6G00387820 Rroxscaffold_70G00446300 Rroxscaffold_70G00446340 Rroxscaffold_70G00446380 Rroxscaffold_71G00445590 Rroxscaffold_71G00445600 Rroxscaffold_71G00445630 Rroxscaffold_71G00445690 Rroxscaffold_71G00445720 Rroxscaffold_71G00445740 Rroxscaffold_71G00445770 Rroxscaffold_72G00449370 Rroxscaffold_72G00449410 Rroxscaffold_72G00449450 Rroxscaffold_73G00439000 Rroxscaffold_73G00439060 Rroxscaffold_73G00439160 Rroxscaffold_73G00439270 Rroxscaffold_74G00442920 Rroxscaffold_75G00447260 Rroxscaffold_75G00447290 Rroxscaffold_75G00447430 Rroxscaffold_76G00448540 Rroxscaffold_76G00448610 Rroxscaffold_77G00449150 Rroxscaffold_78G00449610 Rroxscaffold_79G00450210 Rroxscaffold_80G00450330 Rroxscaffold_80G00450350 Rroxscaffold_81G00450480 Rroxscaffold_83G00450980 Rroxscaffold_85G00451230 Rroxscaffold_86G00451270 Rroxscaffold_89G00451410 Rroxscaffold_90G00448760 Rroxscaffold_90G00448770 Rroxscaffold_90G00448820 Rroxscaffold_91G00443000 Rroxscaffold_91G00443060 Rroxscaffold_92G00446430 Rroxscaffold_93G00440830 Rroxscaffold_93G00440850 Rroxscaffold_93G00440860 Rroxscaffold_93G00440880 Rroxscaffold_93G00440910 Rroxscaffold_94G00445830 Rroxscaffold_94G00445910 Rroxscaffold_94G00445940 Rroxscaffold_94G00446020 Rroxscaffold_97G00442340 Rroxscaffold_97G00442390 Rroxscaffold_97G00442400 Rroxscaffold_98G00451350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 474
AccBSI CCGCTC 2 cut(s) 100, 303
AccI GTMKAC 1 cut(s) 582
AclWI GGATC 2 cut(s) 397, 410
AcoI YGGCCR 4 cut(s) 95, 179, 513, 621
AcsI RAATTY 1 cut(s) 837
AcuI CTGAAG 1 cut(s) 185
AcyI GRCGYC 2 cut(s) 519, 588
AfaI GTAC 2 cut(s) 553, 793
AgsI TTSAA 5 cut(s) 47, 61, 487, 742, 765
AjiI CACGTC 1 cut(s) 254
AjnI CCWGG 2 cut(s) 119, 161
AluBI AGCT 2 cut(s) 417, 507
AluI AGCT 2 cut(s) 417, 507
Alw21I GWGCWC 1 cut(s) 509
Alw26I GTCTC 2 cut(s) 595, 814
AlwI GGATC 2 cut(s) 397, 410
Ama87I CYCGRG 3 cut(s) 106, 612, 653
AoxI GGCC 9 cut(s) 40, 95, 159, 179, 269, 493, 513, 621, 916
ApaI GGGCCC 1 cut(s) 920
ApeKI GCWGC 3 cut(s) 264, 563, 857
ApoI RAATTY 1 cut(s) 837
ArsI GACNNNNNNTTYG 2 cut(s) 230, 262
AsuC2I CCSGG 8 cut(s) 107, 108, 183, 523, 613, 614, 698, 920
AsuHPI GGTGA 1 cut(s) 308
AvaI CYCGRG 3 cut(s) 106, 612, 653
AvaII GGWCC 5 cut(s) 329, 476, 644, 668, 922
BaeGI GKGCMC 1 cut(s) 920
BaeI ACNNNNGTAYC 2 cut(s) 242, 275
BamHI GGATCC 1 cut(s) 402
BanII GRGCYC 3 cut(s) 113, 509, 920
BauI CACGAG 2 cut(s) 395, 893
Bbv12I GWGCWC 1 cut(s) 509
BbvI GCAGC 3 cut(s) 251, 550, 844
BccI CCATC 3 cut(s) 216, 327, 725
BceAI ACGGC 4 cut(s) 110, 194, 570, 601
BcgI CGANNNNNNTGC 4 cut(s) 230, 264, 323, 357
BciT130I CCWGG 2 cut(s) 121, 163
BcnI CCSGG 8 cut(s) 107, 108, 183, 523, 613, 614, 698, 920
BcoDI GTCTC 2 cut(s) 595, 814
BfaI CTAG 1 cut(s) 224
BfoI RGCGCY 3 cut(s) 169, 208, 892
BglI GCCNNNNNGGC 2 cut(s) 178, 554
BglII AGATCT 1 cut(s) 82
Bme18I GGWCC 5 cut(s) 329, 476, 644, 668, 922
BmeT110I CYCGRG 3 cut(s) 106, 612, 653
BmgBI CACGTC 1 cut(s) 254
BmiI GGNNCC 6 cut(s) 25, 160, 330, 331, 404, 918
BmsI GCATC 1 cut(s) 713
Bpu10I CCTNAGC 1 cut(s) 304
BpuMI CCSGG 8 cut(s) 107, 108, 183, 523, 613, 614, 698, 920
BsaHI GRCGYC 2 cut(s) 519, 588
BsaI GGTCTC 1 cut(s) 814
BsaJI CCNNGG 7 cut(s) 106, 119, 472, 594, 612, 697, 828
BsaXI ACNNNNNCTCC 4 cut(s) 84, 114, 575, 605
Bse118I RCCGGY 2 cut(s) 548, 670
Bse1I ACTGG 1 cut(s) 410
BseBI CCWGG 2 cut(s) 121, 163
BseDI CCNNGG 7 cut(s) 106, 119, 472, 594, 612, 697, 828
BseGI GGATG 3 cut(s) 208, 689, 717
BseMII CTCAG 2 cut(s) 318, 376
BseNI ACTGG 1 cut(s) 410
BsePI GCGCGC 4 cut(s) 195, 528, 557, 559
BseSI GKGCMC 1 cut(s) 920
BseX3I CGGCCG 2 cut(s) 95, 179
BseXI GCAGC 3 cut(s) 251, 550, 844
BsgI GTGCAG 1 cut(s) 71
Bsh1285I CGRYCG 8 cut(s) 98, 136, 182, 381, 577, 663, 682, 697
BshFI GGCC 9 cut(s) 42, 97, 161, 181, 271, 495, 515, 623, 918
BsiEI CGRYCG 8 cut(s) 98, 136, 182, 381, 577, 663, 682, 697
BsiHKAI GWGCWC 1 cut(s) 509
BsiHKCI CYCGRG 3 cut(s) 106, 612, 653
BsiSI CCGG 8 cut(s) 107, 182, 522, 549, 613, 671, 697, 920
BslFI GGGAC 1 cut(s) 315
BsmAI GTCTC 2 cut(s) 595, 814
BsmBI CGTCTC 1 cut(s) 595
BsmFI GGGAC 1 cut(s) 315
BsmI GAATGC 1 cut(s) 860
BsnI GGCC 9 cut(s) 42, 97, 161, 181, 271, 495, 515, 623, 918
Bso31I GGTCTC 1 cut(s) 814
BsoBI CYCGRG 3 cut(s) 106, 612, 653
Bsp120I GGGCCC 1 cut(s) 916
Bsp1286I GDGCHC 3 cut(s) 113, 509, 920
Bsp143I GATC 6 cut(s) 82, 368, 402, 439, 574, 660
BspANI GGCC 9 cut(s) 42, 97, 161, 181, 271, 495, 515, 623, 918
BspCNI CTCAG 2 cut(s) 317, 375
BspLI GGNNCC 6 cut(s) 25, 160, 330, 331, 404, 918
BspPI GGATC 2 cut(s) 397, 410
BspTNI GGTCTC 1 cut(s) 814
BsrBI CCGCTC 2 cut(s) 100, 303
BsrFI RCCGGY 2 cut(s) 548, 670
BsrI ACTGG 1 cut(s) 410
BssAI RCCGGY 2 cut(s) 548, 670
BssECI CCNNGG 7 cut(s) 106, 119, 472, 594, 612, 697, 828
BssHII GCGCGC 4 cut(s) 195, 528, 557, 559
BssMI GATC 6 cut(s) 82, 368, 402, 439, 574, 660
BssNI GRCGYC 2 cut(s) 519, 588
BssSI CACGAG 2 cut(s) 395, 893
BssT1I CCWWGG 2 cut(s) 472, 828
Bst2BI CACGAG 2 cut(s) 395, 893
Bst2UI CCWGG 2 cut(s) 121, 163
Bst4CI ACNGT 1 cut(s) 683
Bst6I CTCTTC 1 cut(s) 41
BstACI GRCGYC 2 cut(s) 519, 588
BstDEI CTNAG 2 cut(s) 304, 362
BstDSI CCRYGG 1 cut(s) 594
BstF5I GGATG 3 cut(s) 208, 689, 717
BstH2I RGCGCY 3 cut(s) 169, 208, 892
BstKTI GATC 6 cut(s) 85, 371, 405, 442, 577, 663
BstMAI GTCTC 2 cut(s) 595, 814
BstMBI GATC 6 cut(s) 82, 368, 402, 439, 574, 660
BstMCI CGRYCG 8 cut(s) 98, 136, 182, 381, 577, 663, 682, 697
BstNI CCWGG 2 cut(s) 121, 163
BstNSI RCATGY 2 cut(s) 344, 464
BstSLI GKGCMC 1 cut(s) 920
BstV1I GCAGC 3 cut(s) 251, 550, 844
BstX2I RGATCY 2 cut(s) 82, 402
BstYI RGATCY 2 cut(s) 82, 402
BstZI CGGCCG 2 cut(s) 95, 179
BsuRI GGCC 9 cut(s) 42, 97, 161, 181, 271, 495, 515, 623, 918
BtgI CCRYGG 1 cut(s) 594
BtgZI GCGATG 1 cut(s) 690
BtrI CACGTC 1 cut(s) 254
BtsCI GGATG 3 cut(s) 208, 689, 717
Cfr10I RCCGGY 2 cut(s) 548, 670
Cfr42I CCGCGG 1 cut(s) 597
Cfr9I CCCGGG 2 cut(s) 106, 612
CpoI CGGWCCG 2 cut(s) 668, 922
CseI GACGC 4 cut(s) 527, 568, 577, 628
Csp6I GTAC 2 cut(s) 552, 792
CspCI CAANNNNNGTGG 2 cut(s) 378, 413
CspI CGGWCCG 2 cut(s) 668, 922
CviAII CATG 3 cut(s) 20, 341, 461
CviQI GTAC 2 cut(s) 552, 792
DdeI CTNAG 2 cut(s) 304, 362
DpnI GATC 6 cut(s) 84, 370, 404, 441, 576, 662
DpnII GATC 6 cut(s) 82, 368, 402, 439, 574, 660
DrdI GACNNNNNNGTC 1 cut(s) 474
DseDI GACNNNNNNGTC 1 cut(s) 474
EaeI YGGCCR 4 cut(s) 95, 179, 513, 621
EagI CGGCCG 2 cut(s) 95, 179
Eam1104I CTCTTC 1 cut(s) 41
EarI CTCTTC 1 cut(s) 41
EciI GGCGGA 3 cut(s) 92, 681, 914
Ecl136II GAGCTC 1 cut(s) 507
EclXI CGGCCG 2 cut(s) 95, 179
Eco130I CCWWGG 2 cut(s) 472, 828
Eco24I GRGCYC 3 cut(s) 113, 509, 920
Eco31I GGTCTC 1 cut(s) 814
Eco47I GGWCC 5 cut(s) 329, 476, 644, 668, 922
Eco52I CGGCCG 2 cut(s) 95, 179
Eco53kI GAGCTC 1 cut(s) 507
Eco57I CTGAAG 1 cut(s) 185
Eco88I CYCGRG 3 cut(s) 106, 612, 653
EcoICRI GAGCTC 1 cut(s) 507
EcoO109I RGGNCCY 2 cut(s) 159, 329
EcoRII CCWGG 2 cut(s) 119, 161
EcoT14I CCWWGG 2 cut(s) 472, 828
EcoT38I GRGCYC 3 cut(s) 113, 509, 920
ErhI CCWWGG 2 cut(s) 472, 828
Esp3I CGTCTC 1 cut(s) 595
FaeI CATG 3 cut(s) 23, 344, 464
FaiI YATR 5 cut(s) 21, 311, 342, 462, 602
FaqI GGGAC 1 cut(s) 315
FatI CATG 3 cut(s) 19, 340, 460
FauI CCCGC 4 cut(s) 260, 308, 414, 589
FblI GTMKAC 1 cut(s) 582
FokI GGATG 3 cut(s) 195, 676, 704
FriOI GRGCYC 3 cut(s) 113, 509, 920
FspBI CTAG 1 cut(s) 224
HaeII RGCGCY 3 cut(s) 169, 208, 892
HaeIII GGCC 9 cut(s) 42, 97, 161, 181, 271, 495, 515, 623, 918
HapII CCGG 8 cut(s) 107, 182, 522, 549, 613, 671, 697, 920
HgaI GACGC 4 cut(s) 527, 568, 577, 628
Hin1I GRCGYC 2 cut(s) 519, 588
Hin1II CATG 3 cut(s) 23, 344, 464
HincII GTYRAC 2 cut(s) 450, 583
HindII GTYRAC 2 cut(s) 450, 583
HinfI GANTC 5 cut(s) 230, 451, 468, 754, 877
HpaII CCGG 8 cut(s) 107, 182, 522, 549, 613, 671, 697, 920
HphI GGTGA 1 cut(s) 308
Hpy166II GTNNAC 6 cut(s) 17, 316, 450, 583, 647, 910
Hpy188I TCNGA 3 cut(s) 365, 467, 694
Hpy188III TCNNGA 3 cut(s) 332, 487, 731
Hpy8I GTNNAC 6 cut(s) 17, 316, 450, 583, 647, 910
Hpy99I CGWCG 6 cut(s) 96, 180, 521, 584, 587, 644
HpyAV CCTTC 2 cut(s) 53, 368
HpyCH4III ACNGT 1 cut(s) 683
HpyCH4IV ACGT 1 cut(s) 253
HpyCH4V TGCA 5 cut(s) 6, 88, 251, 389, 860
HpyF3I CTNAG 2 cut(s) 304, 362
HpySE526I ACGT 1 cut(s) 253
Hsp92I GRCGYC 2 cut(s) 519, 588
Hsp92II CATG 3 cut(s) 23, 344, 464
KflI GGGWCCC 1 cut(s) 329
KspI CCGCGG 1 cut(s) 597
Kzo9I GATC 6 cut(s) 82, 368, 402, 439, 574, 660
LmnI GCTCC 3 cut(s) 105, 504, 525
Lsp1109I GCAGC 3 cut(s) 251, 550, 844
LweI GCATC 1 cut(s) 713
MaeI CTAG 1 cut(s) 224
MaeII ACGT 1 cut(s) 253
MalI GATC 6 cut(s) 84, 370, 404, 441, 576, 662
MbiI CCGCTC 2 cut(s) 100, 303
MboI GATC 6 cut(s) 82, 368, 402, 439, 574, 660
MboII GAAGA 2 cut(s) 28, 378
MflI RGATCY 2 cut(s) 82, 402
MhlI GDGCHC 3 cut(s) 113, 509, 920
MluCI AATT 2 cut(s) 736, 837
MlyI GAGTC 4 cut(s) 445, 462, 748, 871
MmeI TCCRAC 1 cut(s) 717
MnlI CCTC 9 cut(s) 44, 155, 282, 297, 404, 578, 738, 795, 902
MspA1I CMGCKG 2 cut(s) 596, 930
MspI CCGG 8 cut(s) 107, 182, 522, 549, 613, 671, 697, 920
Mva1269I GAATGC 1 cut(s) 860
MvaI CCWGG 2 cut(s) 121, 163
NciI CCSGG 8 cut(s) 107, 108, 183, 523, 613, 614, 698, 920
NdeII GATC 6 cut(s) 82, 368, 402, 439, 574, 660
NlaIII CATG 3 cut(s) 23, 344, 464
NlaIV GGNNCC 6 cut(s) 25, 160, 330, 331, 404, 918
NspI RCATGY 2 cut(s) 344, 464
PaeI GCATGC 1 cut(s) 344
PauI GCGCGC 4 cut(s) 195, 528, 557, 559
PcsI WCGNNNNNNNCGW 2 cut(s) 250, 579
PctI GAATGC 1 cut(s) 860
PfeI GAWTC 1 cut(s) 230
PflFI GACNNNGTC 1 cut(s) 587
Ple19I CGATCG 2 cut(s) 577, 663
PleI GAGTC 4 cut(s) 445, 462, 748, 871
PpsI GAGTC 4 cut(s) 445, 462, 748, 871
PpuMI RGGWCCY 1 cut(s) 329
Psp124BI GAGCTC 1 cut(s) 509
Psp5II RGGWCCY 1 cut(s) 329
Psp6I CCWGG 2 cut(s) 119, 161
PspGI CCWGG 2 cut(s) 119, 161
PspN4I GGNNCC 6 cut(s) 25, 160, 330, 331, 404, 918
PspOMI GGGCCC 1 cut(s) 916
PspPPI RGGWCCY 1 cut(s) 329
PsuI RGATCY 2 cut(s) 82, 402
PsyI GACNNNGTC 1 cut(s) 587
PteI GCGCGC 4 cut(s) 195, 528, 557, 559
PvuI CGATCG 2 cut(s) 577, 663
RsaI GTAC 2 cut(s) 553, 793
RsaNI GTAC 2 cut(s) 552, 792
Rsr2I CGGWCCG 2 cut(s) 668, 922
RsrII CGGWCCG 2 cut(s) 668, 922
SacI GAGCTC 1 cut(s) 509
SacII CCGCGG 1 cut(s) 597
SalI GTCGAC 1 cut(s) 581
Sau3AI GATC 6 cut(s) 82, 368, 402, 439, 574, 660
SchI GAGTC 4 cut(s) 445, 462, 748, 871
SduI GDGCHC 3 cut(s) 113, 509, 920
SetI ASST 8 cut(s) 29, 125, 193, 256, 377, 419, 509, 909
SfaNI GCATC 1 cut(s) 713
Sfr303I CCGCGG 1 cut(s) 597
SgrBI CCGCGG 1 cut(s) 597
SgrDI CGTCGACG 1 cut(s) 581
SinI GGWCC 5 cut(s) 329, 476, 644, 668, 922
SmaI CCCGGG 2 cut(s) 108, 614
SphI GCATGC 1 cut(s) 344
SrfI GCCCGGGC 2 cut(s) 108, 614
Sse9I AATT 2 cut(s) 736, 837
SspI AATATT 1 cut(s) 64
SspMI CTAG 1 cut(s) 224
SstI GAGCTC 1 cut(s) 509
StyI CCWWGG 2 cut(s) 472, 828
TaaI ACNGT 1 cut(s) 683
TaiI ACGT 1 cut(s) 256
TaqI TCGA 3 cut(s) 352, 582, 678
TaqII GACCGA 3 cut(s) 367, 632, 796
TasI AATT 2 cut(s) 736, 837
TauI GCSGC 7 cut(s) 100, 132, 141, 626, 629, 632, 930
TfiI GAWTC 1 cut(s) 230
TseI GCWGC 3 cut(s) 264, 563, 857
TspDTI ATGAA 1 cut(s) 765
TspGWI ACGGA 1 cut(s) 468
TspMI CCCGGG 2 cut(s) 106, 612
Tth111I GACNNNGTC 1 cut(s) 587
VpaK11BI GGWCC 5 cut(s) 329, 476, 644, 668, 922
XapI RAATTY 1 cut(s) 837
XceI RCATGY 2 cut(s) 344, 464
XmaI CCCGGG 2 cut(s) 106, 612
XmiI GTMKAC 1 cut(s) 582
XspI CTAG 1 cut(s) 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.