Rroxscaffold_18G00446130

mitotic sister chromatid biorientation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000018
OG0000098
Physical Location & Seq
Forward (+)
53873 .. 54656
784 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_18G00446130.1

Sequence Viewer

Length: 606 bp
ATGAGTACGGCCGGGCGTGAATGGCACTCGGTCCTCCGGATTTTCAAGGGCCGCCGGGGCGCACGGACACCACGCGACGTGCGGTGCTCTTCCGGCCGCTGGACCCTACCTCCGGCTGAGCCGTTTCCGGGGTGGGCGGTGTTAAACAGAAAAGATAACTCTTCCCGAGGCCCCGCCGACGTCTCCGGACTCCCTAACGTTGCCGTCAACCACCACGTCCCGGATCGACTAACCCATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATCTGCACCGACGGCCGCTCCGCCGGGCTCACGCCCCGGGTTTTGCGGCGACCGCCGCGCCCTCCTACTCATCGGGGCACTGGCACTTGCCCCGACGGCGGGTATAGGTCACGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCGTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCGGTTCTGCTTACCAAAAATGGCCCACTTGGAGCTCTCGATTCCTTGGCGTGGCTCAACAAAGCAGCCACGCCGTCCTACCTATTTAAAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

201

Amino Acids

21.63

Weight (kDa)

11.36

Isoelectric Point (pI)

53.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000098)

Species Orthologous Gene IDs
pyrus_communis pycom05g23460 pycom581g00100 pycom849g00040
rosa_multiflora Rmu_sc0019961.1_g000001
rosa_roxburghii Rroxscaffold_100G00451180 Rroxscaffold_102G00450890 Rroxscaffold_102G00450920 Rroxscaffold_103G00450530 Rroxscaffold_103G00450560 Rroxscaffold_104G00444830 Rroxscaffold_104G00444900 Rroxscaffold_105G00447100 Rroxscaffold_105G00447180 Rroxscaffold_107G00442470 Rroxscaffold_107G00442480 Rroxscaffold_107G00442520 Rroxscaffold_107G00442540 Rroxscaffold_107G00442560 Rroxscaffold_107G00442570 Rroxscaffold_107G00442600 Rroxscaffold_107G00442630 Rroxscaffold_107G00442650 Rroxscaffold_107G00442660 Rroxscaffold_108G00451480 Rroxscaffold_110G00451510 Rroxscaffold_111G00451550 Rroxscaffold_112G00451590 Rroxscaffold_113G00451630 Rroxscaffold_114G00451650 Rroxscaffold_116G00451710 Rroxscaffold_117G00451740 Rroxscaffold_117G00451750 Rroxscaffold_121G00451840 Rroxscaffold_122G00451860 Rroxscaffold_122G00451880 Rroxscaffold_123G00451920 Rroxscaffold_124G00451950 Rroxscaffold_128G00452040 Rroxscaffold_128G00452060 Rroxscaffold_12G00450600 Rroxscaffold_12G00450620 Rroxscaffold_131G00452110 Rroxscaffold_138G00452180 Rroxscaffold_13G00448630 Rroxscaffold_145G00452240 Rroxscaffold_14G00441930 Rroxscaffold_14G00441950 Rroxscaffold_14G00442050 Rroxscaffold_14G00442080 Rroxscaffold_15G00447500 Rroxscaffold_162G00450770 Rroxscaffold_16G00446200 Rroxscaffold_16G00446210 Rroxscaffold_16G00446240 Rroxscaffold_17G00435360 Rroxscaffold_17G00435410 Rroxscaffold_17G00435450 Rroxscaffold_17G00435480 Rroxscaffold_17G00435500 Rroxscaffold_17G00435600 Rroxscaffold_17G00435660 Rroxscaffold_17G00435680 Rroxscaffold_17G00435760 Rroxscaffold_17G00435780 Rroxscaffold_17G00435810 Rroxscaffold_17G00435840 Rroxscaffold_17G00435870 Rroxscaffold_18G00446030 Rroxscaffold_18G00446060 Rroxscaffold_18G00446130 Rroxscaffold_19G00448890 Rroxscaffold_19G00448920 Rroxscaffold_19G00448940 Rroxscaffold_19G00448970 Rroxscaffold_1G00000020 Rroxscaffold_1G00000050 Rroxscaffold_1G00000160 Rroxscaffold_20G00445060 Rroxscaffold_20G00445100 Rroxscaffold_20G00445130 Rroxscaffold_21G00439490 Rroxscaffold_21G00439560 Rroxscaffold_21G00439580 Rroxscaffold_21G00439620 Rroxscaffold_21G00439630 Rroxscaffold_22G00439910 Rroxscaffold_22G00439950 Rroxscaffold_23G00451020 Rroxscaffold_23G00451040 Rroxscaffold_23G00451070 Rroxscaffold_24G00444920 Rroxscaffold_24G00444950 Rroxscaffold_24G00445000 Rroxscaffold_25G00450200 Rroxscaffold_26G00447730 Rroxscaffold_27G00446570 Rroxscaffold_27G00446600 Rroxscaffold_28G00446830 Rroxscaffold_28G00446860 Rroxscaffold_28G00446910 Rroxscaffold_29G00441560 Rroxscaffold_29G00441570 Rroxscaffold_29G00441590 Rroxscaffold_29G00441630 Rroxscaffold_29G00441640 Rroxscaffold_29G00441670 Rroxscaffold_29G00441680 Rroxscaffold_29G00441860 Rroxscaffold_30G00449540 Rroxscaffold_30G00449570 Rroxscaffold_30G00449580 Rroxscaffold_31G00438120 Rroxscaffold_31G00438140 Rroxscaffold_31G00438180 Rroxscaffold_31G00438190 Rroxscaffold_31G00438220 Rroxscaffold_31G00438230 Rroxscaffold_32G00442740 Rroxscaffold_32G00442760 Rroxscaffold_33G00439670 Rroxscaffold_33G00439720 Rroxscaffold_33G00439740 Rroxscaffold_33G00439760 Rroxscaffold_33G00439820 Rroxscaffold_34G00443140 Rroxscaffold_34G00443200 Rroxscaffold_34G00443250 Rroxscaffold_34G00443370 Rroxscaffold_34G00443390 Rroxscaffold_35G00441050 Rroxscaffold_35G00441080 Rroxscaffold_35G00441090 Rroxscaffold_35G00441170 Rroxscaffold_35G00441190 Rroxscaffold_35G00441230 Rroxscaffold_36G00440100 Rroxscaffold_36G00440170 Rroxscaffold_37G00445170 Rroxscaffold_38G00444440 Rroxscaffold_38G00444500 Rroxscaffold_38G00444550 Rroxscaffold_39G00448200 Rroxscaffold_39G00448210 Rroxscaffold_40G00447650 Rroxscaffold_40G00447680 Rroxscaffold_40G00447700 Rroxscaffold_40G00447710 Rroxscaffold_41G00451110 Rroxscaffold_41G00451130 Rroxscaffold_42G00450430 Rroxscaffold_43G00449800 Rroxscaffold_43G00449860 Rroxscaffold_43G00449920 Rroxscaffold_43G00449930 Rroxscaffold_44G00440540 Rroxscaffold_44G00440580 Rroxscaffold_44G00440660 Rroxscaffold_45G00438900 Rroxscaffold_45G00438930 Rroxscaffold_45G00438950 Rroxscaffold_47G00444020 Rroxscaffold_47G00444060 Rroxscaffold_47G00444120 Rroxscaffold_48G00448450 Rroxscaffold_49G00438570 Rroxscaffold_49G00438650 Rroxscaffold_49G00438700 Rroxscaffold_4G00321080 Rroxscaffold_50G00444130 Rroxscaffold_50G00444200 Rroxscaffold_50G00444210 Rroxscaffold_50G00444330 Rroxscaffold_50G00444350 Rroxscaffold_51G00447840 Rroxscaffold_52G00439310 Rroxscaffold_52G00439390 Rroxscaffold_54G00443660 Rroxscaffold_55G00450020 Rroxscaffold_55G00450050 Rroxscaffold_55G00450060 Rroxscaffold_55G00450080 Rroxscaffold_55G00450090 Rroxscaffold_55G00450120 Rroxscaffold_57G00443070 Rroxscaffold_59G00442210 Rroxscaffold_59G00442220 Rroxscaffold_60G00448360 Rroxscaffold_60G00448370 Rroxscaffold_60G00448410 Rroxscaffold_61G00449990 Rroxscaffold_62G00438040 Rroxscaffold_62G00438060 Rroxscaffold_62G00438080 Rroxscaffold_63G00444410 Rroxscaffold_63G00444430 Rroxscaffold_64G00450670 Rroxscaffold_65G00445300 Rroxscaffold_65G00445340 Rroxscaffold_65G00445360 Rroxscaffold_65G00445380 Rroxscaffold_65G00445420 Rroxscaffold_66G00437660 Rroxscaffold_66G00437820 Rroxscaffold_66G00437840 Rroxscaffold_66G00437890 Rroxscaffold_67G00448140 Rroxscaffold_67G00448150 Rroxscaffold_68G00447010 Rroxscaffold_69G00446670 Rroxscaffold_69G00446710 Rroxscaffold_69G00446770 Rroxscaffold_6G00387820 Rroxscaffold_70G00446300 Rroxscaffold_70G00446340 Rroxscaffold_70G00446380 Rroxscaffold_71G00445590 Rroxscaffold_71G00445600 Rroxscaffold_71G00445630 Rroxscaffold_71G00445690 Rroxscaffold_71G00445720 Rroxscaffold_71G00445740 Rroxscaffold_71G00445770 Rroxscaffold_72G00449370 Rroxscaffold_72G00449410 Rroxscaffold_72G00449450 Rroxscaffold_73G00439000 Rroxscaffold_73G00439060 Rroxscaffold_73G00439160 Rroxscaffold_73G00439270 Rroxscaffold_74G00442920 Rroxscaffold_75G00447260 Rroxscaffold_75G00447290 Rroxscaffold_75G00447430 Rroxscaffold_76G00448540 Rroxscaffold_76G00448610 Rroxscaffold_77G00449150 Rroxscaffold_78G00449610 Rroxscaffold_79G00450210 Rroxscaffold_80G00450330 Rroxscaffold_80G00450350 Rroxscaffold_81G00450480 Rroxscaffold_83G00450980 Rroxscaffold_85G00451230 Rroxscaffold_86G00451270 Rroxscaffold_89G00451410 Rroxscaffold_90G00448760 Rroxscaffold_90G00448770 Rroxscaffold_90G00448820 Rroxscaffold_91G00443000 Rroxscaffold_91G00443060 Rroxscaffold_92G00446430 Rroxscaffold_93G00440830 Rroxscaffold_93G00440850 Rroxscaffold_93G00440860 Rroxscaffold_93G00440880 Rroxscaffold_93G00440910 Rroxscaffold_94G00445830 Rroxscaffold_94G00445910 Rroxscaffold_94G00445940 Rroxscaffold_94G00446020 Rroxscaffold_97G00442340 Rroxscaffold_97G00442390 Rroxscaffold_97G00442400 Rroxscaffold_98G00451350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 183
AccB1I GGYRCC 1 cut(s) 476
AccBSI CCGCTC 1 cut(s) 335
AccII CGCG 3 cut(s) 75, 375, 431
AccIII TCCGGA 2 cut(s) 36, 185
AclI AACGTT 1 cut(s) 198
AclWI GGATC 1 cut(s) 231
AcoI YGGCCR 3 cut(s) 9, 94, 330
AcyI GRCGYC 1 cut(s) 180
AfaI GTAC 1 cut(s) 7
AflII CTTAAG 1 cut(s) 434
AgsI TTSAA 3 cut(s) 46, 282, 296
AjiI CACGTC 2 cut(s) 79, 217
AluBI AGCT 1 cut(s) 545
AluI AGCT 1 cut(s) 545
Alw21I GWGCWC 2 cut(s) 89, 547
Alw26I GTCTC 1 cut(s) 187
AlwI GGATC 1 cut(s) 231
Ama87I CYCGRG 2 cut(s) 165, 353
Aor13HI TCCGGA 2 cut(s) 36, 185
AoxI GGCC 7 cut(s) 9, 49, 94, 169, 275, 330, 532
ApeKI GCWGC 1 cut(s) 575
AspLEI GCGC 4 cut(s) 62, 377, 433, 441
AspS9I GGNCC 5 cut(s) 31, 49, 102, 170, 533
AsuC2I CCSGG 8 cut(s) 13, 56, 129, 221, 342, 354, 355, 487
AvaI CYCGRG 2 cut(s) 165, 353
AvaII GGWCC 2 cut(s) 31, 102
BaeGI GKGCMC 2 cut(s) 397, 479
BanI GGYRCC 1 cut(s) 476
BanII GRGCYC 2 cut(s) 347, 547
BarI GAAGNNNNNNTAC 2 cut(s) 474, 506
Bbv12I GWGCWC 2 cut(s) 89, 547
BbvI GCAGC 1 cut(s) 587
BccI CCATC 1 cut(s) 450
BceAI ACGGC 7 cut(s) 24, 106, 188, 232, 345, 429, 568
BcnI CCSGG 8 cut(s) 13, 56, 129, 221, 342, 354, 355, 487
BcoDI GTCTC 1 cut(s) 187
BfaI CTAG 1 cut(s) 458
BfoI RGCGCY 1 cut(s) 442
BfrI CTTAAG 1 cut(s) 434
BglI GCCNNNNNGGC 2 cut(s) 57, 413
BglII AGATCT 1 cut(s) 317
BisI GCNGC 6 cut(s) 52, 97, 333, 364, 373, 576
BlpI GCTNAGC 1 cut(s) 117
BlsI GCNGC 6 cut(s) 53, 98, 334, 365, 374, 577
Bme1390I CCNGG 8 cut(s) 13, 56, 129, 221, 342, 354, 355, 487
Bme18I GGWCC 2 cut(s) 31, 102
BmeT110I CYCGRG 2 cut(s) 165, 353
BmgBI CACGTC 2 cut(s) 79, 217
BmgT120I GGNCC 5 cut(s) 31, 49, 102, 170, 533
BmiI GGNNCC 4 cut(s) 104, 172, 260, 478
BmrFI CCNGG 8 cut(s) 13, 56, 129, 221, 342, 354, 355, 487
BmsI GCATC 1 cut(s) 515
Bpu1102I GCTNAGC 1 cut(s) 117
BpuMI CCSGG 8 cut(s) 13, 56, 129, 221, 342, 354, 355, 487
BsaHI GRCGYC 1 cut(s) 180
BsaJI CCNNGG 6 cut(s) 55, 128, 166, 352, 353, 555
BsaWI WCCGGW 3 cut(s) 36, 185, 493
BsaXI ACNNNNNCTCC 4 cut(s) 94, 124, 319, 349
Bse118I RCCGGY 1 cut(s) 511
Bse1I ACTGG 1 cut(s) 402
BseAI TCCGGA 2 cut(s) 36, 185
BseDI CCNNGG 6 cut(s) 55, 128, 166, 352, 353, 555
BseGI GGATG 2 cut(s) 442, 499
BseMII CTCAG 1 cut(s) 108
BseNI ACTGG 1 cut(s) 402
BseSI GKGCMC 2 cut(s) 397, 479
BseX3I CGGCCG 3 cut(s) 9, 94, 330
BseXI GCAGC 1 cut(s) 587
BsgI GTGCAG 1 cut(s) 306
Bsh1236I CGCG 3 cut(s) 75, 375, 431
Bsh1285I CGRYCG 4 cut(s) 12, 97, 333, 370
BshFI GGCC 7 cut(s) 11, 51, 96, 171, 277, 332, 534
BshNI GGYRCC 1 cut(s) 476
BsiEI CGRYCG 4 cut(s) 12, 97, 333, 370
BsiHKAI GWGCWC 2 cut(s) 89, 547
BsiHKCI CYCGRG 2 cut(s) 165, 353
BslFI GGGAC 1 cut(s) 203
BsmAI GTCTC 1 cut(s) 187
BsmBI CGTCTC 1 cut(s) 187
BsmFI GGGAC 1 cut(s) 203
BsnI GGCC 7 cut(s) 11, 51, 96, 171, 277, 332, 534
BsoBI CYCGRG 2 cut(s) 165, 353
Bsp1286I GDGCHC 5 cut(s) 89, 347, 397, 479, 547
Bsp13I TCCGGA 2 cut(s) 36, 185
Bsp143I GATC 2 cut(s) 223, 317
Bsp1720I GCTNAGC 1 cut(s) 117
BspANI GGCC 7 cut(s) 11, 51, 96, 171, 277, 332, 534
BspCNI CTCAG 1 cut(s) 109
BspEI TCCGGA 2 cut(s) 36, 185
BspFNI CGCG 3 cut(s) 75, 375, 431
BspLI GGNNCC 4 cut(s) 104, 172, 260, 478
BspPI GGATC 1 cut(s) 231
BspQI GCTCTTC 1 cut(s) 94
BspT107I GGYRCC 1 cut(s) 476
BspTI CTTAAG 1 cut(s) 434
BsrBI CCGCTC 1 cut(s) 335
BsrFI RCCGGY 1 cut(s) 511
BsrI ACTGG 1 cut(s) 402
BssAI RCCGGY 1 cut(s) 511
BssECI CCNNGG 6 cut(s) 55, 128, 166, 352, 353, 555
BssMI GATC 2 cut(s) 223, 317
BssNI GRCGYC 1 cut(s) 180
BssT1I CCWWGG 1 cut(s) 555
Bst4CI ACNGT 1 cut(s) 481
Bst6I CTCTTC 3 cut(s) 94, 166, 276
BstACI GRCGYC 1 cut(s) 180
BstAFI CTTAAG 1 cut(s) 434
BstDEI CTNAG 1 cut(s) 117
BstF5I GGATG 2 cut(s) 442, 499
BstFNI CGCG 3 cut(s) 75, 375, 431
BstH2I RGCGCY 1 cut(s) 442
BstHHI GCGC 4 cut(s) 62, 377, 433, 441
BstKTI GATC 2 cut(s) 226, 320
BstMAI GTCTC 1 cut(s) 187
BstMBI GATC 2 cut(s) 223, 317
BstMCI CGRYCG 4 cut(s) 12, 97, 333, 370
BstMWI GCNNNNNNNGC 8 cut(s) 22, 57, 93, 329, 369, 372, 413, 517
BstSCI CCNGG 8 cut(s) 11, 54, 127, 219, 340, 352, 353, 485
BstSLI GKGCMC 2 cut(s) 397, 479
BstUI CGCG 3 cut(s) 75, 375, 431
BstV1I GCAGC 1 cut(s) 587
BstX2I RGATCY 1 cut(s) 317
BstYI RGATCY 1 cut(s) 317
BstZI CGGCCG 3 cut(s) 9, 94, 330
BsuRI GGCC 7 cut(s) 11, 51, 96, 171, 277, 332, 534
BtgZI GCGATG 1 cut(s) 492
BtrI CACGTC 2 cut(s) 79, 217
BtsCI GGATG 2 cut(s) 442, 499
BtsIMutI CAGTG 1 cut(s) 395
CfoI GCGC 4 cut(s) 62, 377, 433, 441
Cfr10I RCCGGY 1 cut(s) 511
Cfr13I GGNCC 5 cut(s) 31, 49, 102, 170, 533
Cfr9I CCCGGG 1 cut(s) 353
Csp6I GTAC 1 cut(s) 6
CviAII CATG 2 cut(s) 236, 255
CviQI GTAC 1 cut(s) 6
DdeI CTNAG 1 cut(s) 117
DpnI GATC 2 cut(s) 225, 319
DpnII GATC 2 cut(s) 223, 317
DraI TTTAAA 1 cut(s) 598
EaeI YGGCCR 3 cut(s) 9, 94, 330
EagI CGGCCG 3 cut(s) 9, 94, 330
Eam1104I CTCTTC 3 cut(s) 94, 166, 276
EarI CTCTTC 3 cut(s) 94, 166, 276
EciI GGCGGA 1 cut(s) 327
Ecl136II GAGCTC 1 cut(s) 545
EclXI CGGCCG 3 cut(s) 9, 94, 330
Eco130I CCWWGG 1 cut(s) 555
Eco24I GRGCYC 2 cut(s) 347, 547
Eco47I GGWCC 2 cut(s) 31, 102
Eco52I CGGCCG 3 cut(s) 9, 94, 330
Eco53kI GAGCTC 1 cut(s) 545
Eco88I CYCGRG 2 cut(s) 165, 353
EcoICRI GAGCTC 1 cut(s) 545
EcoO109I RGGNCCY 1 cut(s) 170
EcoT14I CCWWGG 1 cut(s) 555
EcoT38I GRGCYC 2 cut(s) 347, 547
ErhI CCWWGG 1 cut(s) 555
Esp3I CGTCTC 1 cut(s) 187
FaeI CATG 2 cut(s) 239, 258
FaiI YATR 3 cut(s) 237, 256, 422
FaqI GGGAC 1 cut(s) 203
FatI CATG 2 cut(s) 235, 254
FauI CCCGC 3 cut(s) 181, 409, 511
Fnu4HI GCNGC 6 cut(s) 52, 97, 333, 364, 373, 576
FokI GGATG 2 cut(s) 429, 486
FriOI GRGCYC 2 cut(s) 347, 547
Fsp4HI GCNGC 6 cut(s) 52, 97, 333, 364, 373, 576
FspBI CTAG 1 cut(s) 458
GlaI GCGC 4 cut(s) 61, 376, 432, 440
GluI GCNGC 6 cut(s) 52, 97, 333, 364, 373, 576
HaeII RGCGCY 1 cut(s) 442
HaeIII GGCC 7 cut(s) 11, 51, 96, 171, 277, 332, 534
HhaI GCGC 4 cut(s) 62, 377, 433, 441
Hin1I GRCGYC 1 cut(s) 180
Hin1II CATG 2 cut(s) 239, 258
Hin6I GCGC 4 cut(s) 60, 375, 431, 439
HinP1I GCGC 4 cut(s) 60, 375, 431, 439
HincII GTYRAC 1 cut(s) 208
HindII GTYRAC 1 cut(s) 208
HinfI GANTC 3 cut(s) 189, 464, 551
Hpy166II GTNNAC 2 cut(s) 208, 252
Hpy188III TCNNGA 4 cut(s) 37, 165, 186, 548
Hpy8I GTNNAC 2 cut(s) 208, 252
Hpy99I CGWCG 4 cut(s) 80, 182, 331, 415
HpyAV CCTTC 1 cut(s) 288
HpyCH4III ACNGT 1 cut(s) 481
HpyCH4IV ACGT 4 cut(s) 78, 180, 198, 216
HpyCH4V TGCA 2 cut(s) 241, 323
HpyF10VI GCNNNNNNNGC 8 cut(s) 22, 57, 93, 329, 369, 372, 413, 517
HpyF3I CTNAG 1 cut(s) 117
HpySE526I ACGT 4 cut(s) 78, 180, 198, 216
Hsp92I GRCGYC 1 cut(s) 180
Hsp92II CATG 2 cut(s) 239, 258
HspAI GCGC 4 cut(s) 60, 375, 431, 439
Kpn2I TCCGGA 2 cut(s) 36, 185
Kzo9I GATC 2 cut(s) 223, 317
LguI GCTCTTC 1 cut(s) 94
LmnI GCTCC 2 cut(s) 340, 542
Lsp1109I GCAGC 1 cut(s) 587
LweI GCATC 1 cut(s) 515
MaeI CTAG 1 cut(s) 458
MaeII ACGT 4 cut(s) 78, 180, 198, 216
MaeIII GTNAC 2 cut(s) 425, 481
MalI GATC 2 cut(s) 225, 319
MbiI CCGCTC 1 cut(s) 335
MboI GATC 2 cut(s) 223, 317
MboII GAAGA 3 cut(s) 81, 153, 263
MflI RGATCY 1 cut(s) 317
MhlI GDGCHC 5 cut(s) 89, 347, 397, 479, 547
MlyI GAGTC 1 cut(s) 183
MnlI CCTC 5 cut(s) 44, 120, 161, 279, 389
MroI TCCGGA 2 cut(s) 36, 185
MseI TTAA 3 cut(s) 143, 435, 597
MspA1I CMGCKG 1 cut(s) 99
MspCI CTTAAG 1 cut(s) 434
MspR9I CCNGG 8 cut(s) 13, 56, 129, 221, 342, 354, 355, 487
MvnI CGCG 3 cut(s) 75, 375, 431
MwoI GCNNNNNNNGC 8 cut(s) 22, 57, 93, 329, 369, 372, 413, 517
NciI CCSGG 8 cut(s) 13, 56, 129, 221, 342, 354, 355, 487
NdeII GATC 2 cut(s) 223, 317
NlaIII CATG 2 cut(s) 239, 258
NlaIV GGNNCC 4 cut(s) 104, 172, 260, 478
NmuCI GTSAC 1 cut(s) 425
PciSI GCTCTTC 1 cut(s) 94
PfeI GAWTC 2 cut(s) 464, 551
PfoI TCCNGGA 1 cut(s) 219
PkrI GCNGC 6 cut(s) 53, 98, 334, 365, 374, 577
PleI GAGTC 1 cut(s) 183
PpsI GAGTC 1 cut(s) 183
Psp124BI GAGCTC 1 cut(s) 547
Psp1406I AACGTT 1 cut(s) 198
PspN4I GGNNCC 4 cut(s) 104, 172, 260, 478
PspPI GGNCC 5 cut(s) 31, 49, 102, 170, 533
PsuI RGATCY 1 cut(s) 317
RsaI GTAC 1 cut(s) 7
RsaNI GTAC 1 cut(s) 6
SacI GAGCTC 1 cut(s) 547
SapI GCTCTTC 1 cut(s) 94
SaqAI TTAA 3 cut(s) 143, 435, 597
SatI GCNGC 6 cut(s) 52, 97, 333, 364, 373, 576
Sau3AI GATC 2 cut(s) 223, 317
Sau96I GGNCC 5 cut(s) 31, 49, 102, 170, 533
SchI GAGTC 1 cut(s) 183
ScrFI CCNGG 8 cut(s) 13, 56, 129, 221, 342, 354, 355, 487
SduI GDGCHC 5 cut(s) 89, 347, 397, 479, 547
SetI ASST 9 cut(s) 81, 112, 183, 201, 219, 264, 427, 547, 594
SfaNI GCATC 1 cut(s) 515
SinI GGWCC 2 cut(s) 31, 102
SmaI CCCGGG 1 cut(s) 355
SmlI CTYRAG 1 cut(s) 434
SmoI CTYRAG 1 cut(s) 434
SspI AATATT 1 cut(s) 299
SspMI CTAG 1 cut(s) 458
SstI GAGCTC 1 cut(s) 547
StyD4I CCNGG 8 cut(s) 11, 54, 127, 219, 340, 352, 353, 485
StyI CCWWGG 1 cut(s) 555
TaaI ACNGT 1 cut(s) 481
TaiI ACGT 4 cut(s) 81, 183, 201, 219
TaqI TCGA 2 cut(s) 226, 549
TaqII GACCGA 1 cut(s) 19
TauI GCSGC 5 cut(s) 54, 99, 335, 366, 375
TfiI GAWTC 2 cut(s) 464, 551
Tru1I TTAA 3 cut(s) 143, 435, 597
Tru9I TTAA 3 cut(s) 143, 435, 597
TscAI CASTG 1 cut(s) 402
TseFI GTSAC 1 cut(s) 425
TseI GCWGC 1 cut(s) 575
Tsp45I GTSAC 1 cut(s) 425
TspDTI ATGAA 1 cut(s) 488
TspGWI ACGGA 1 cut(s) 79
TspMI CCCGGG 1 cut(s) 353
TspRI CASTG 1 cut(s) 402
Vha464I CTTAAG 1 cut(s) 434
VpaK11BI GGWCC 2 cut(s) 31, 102
XmaI CCCGGG 1 cut(s) 353
XspI CTAG 1 cut(s) 458
ZraI GACGTC 1 cut(s) 181
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.