Rroxscaffold_35G00441080

DNA binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000035
OG0000098
Physical Location & Seq
Forward (+)
25529 .. 26268
740 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_35G00441080.1

Sequence Viewer

Length: 591 bp
ATGACCAATTGTGCGAATCAACGGAAGAGCCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCATTATCCCTGTGGTAACTTTTCTGACACCTCTAGCTTTAAATTCCAAAGGTCTAAAGGATCGATAGGCCACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATTTCTGTTCTCGTTGAGCTCATCTTAGGACACCTGCGTTATCTTTTAACGGATGTGCCGCCCCAGCCAAACTCCCCACCTGACAATGTCTTCCGCCCGGATCAGCCCGCCGAAGCAGGCTTTGGGTCCAAAAAGAGGGGCAGTGCCCCGCCTCCGATTCACGGAATAAGTAAAATAACGTTAAAAGTAGTGGTATTTCACTTTCGCCCGAAGGCTCCCACTTATACTACACCTCTCAAGTCATTTCACAAAGTCGGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGACAGGGACAGTGGGAATCTCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

196

Amino Acids

21.88

Weight (kDa)

10.49

Isoelectric Point (pI)

49.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000098)

Species Orthologous Gene IDs
pyrus_communis pycom05g23460 pycom581g00100 pycom849g00040
rosa_multiflora Rmu_sc0019961.1_g000001
rosa_roxburghii Rroxscaffold_100G00451180 Rroxscaffold_102G00450890 Rroxscaffold_102G00450920 Rroxscaffold_103G00450530 Rroxscaffold_103G00450560 Rroxscaffold_104G00444830 Rroxscaffold_104G00444900 Rroxscaffold_105G00447100 Rroxscaffold_105G00447180 Rroxscaffold_107G00442470 Rroxscaffold_107G00442480 Rroxscaffold_107G00442520 Rroxscaffold_107G00442540 Rroxscaffold_107G00442560 Rroxscaffold_107G00442570 Rroxscaffold_107G00442600 Rroxscaffold_107G00442630 Rroxscaffold_107G00442650 Rroxscaffold_107G00442660 Rroxscaffold_108G00451480 Rroxscaffold_110G00451510 Rroxscaffold_111G00451550 Rroxscaffold_112G00451590 Rroxscaffold_113G00451630 Rroxscaffold_114G00451650 Rroxscaffold_116G00451710 Rroxscaffold_117G00451740 Rroxscaffold_117G00451750 Rroxscaffold_121G00451840 Rroxscaffold_122G00451860 Rroxscaffold_122G00451880 Rroxscaffold_123G00451920 Rroxscaffold_124G00451950 Rroxscaffold_128G00452040 Rroxscaffold_128G00452060 Rroxscaffold_12G00450600 Rroxscaffold_12G00450620 Rroxscaffold_131G00452110 Rroxscaffold_138G00452180 Rroxscaffold_13G00448630 Rroxscaffold_145G00452240 Rroxscaffold_14G00441930 Rroxscaffold_14G00441950 Rroxscaffold_14G00442050 Rroxscaffold_14G00442080 Rroxscaffold_15G00447500 Rroxscaffold_162G00450770 Rroxscaffold_16G00446200 Rroxscaffold_16G00446210 Rroxscaffold_16G00446240 Rroxscaffold_17G00435360 Rroxscaffold_17G00435410 Rroxscaffold_17G00435450 Rroxscaffold_17G00435480 Rroxscaffold_17G00435500 Rroxscaffold_17G00435600 Rroxscaffold_17G00435660 Rroxscaffold_17G00435680 Rroxscaffold_17G00435760 Rroxscaffold_17G00435780 Rroxscaffold_17G00435810 Rroxscaffold_17G00435840 Rroxscaffold_17G00435870 Rroxscaffold_18G00446030 Rroxscaffold_18G00446060 Rroxscaffold_18G00446130 Rroxscaffold_19G00448890 Rroxscaffold_19G00448920 Rroxscaffold_19G00448940 Rroxscaffold_19G00448970 Rroxscaffold_1G00000020 Rroxscaffold_1G00000050 Rroxscaffold_1G00000160 Rroxscaffold_20G00445060 Rroxscaffold_20G00445100 Rroxscaffold_20G00445130 Rroxscaffold_21G00439490 Rroxscaffold_21G00439560 Rroxscaffold_21G00439580 Rroxscaffold_21G00439620 Rroxscaffold_21G00439630 Rroxscaffold_22G00439910 Rroxscaffold_22G00439950 Rroxscaffold_23G00451020 Rroxscaffold_23G00451040 Rroxscaffold_23G00451070 Rroxscaffold_24G00444920 Rroxscaffold_24G00444950 Rroxscaffold_24G00445000 Rroxscaffold_25G00450200 Rroxscaffold_26G00447730 Rroxscaffold_27G00446570 Rroxscaffold_27G00446600 Rroxscaffold_28G00446830 Rroxscaffold_28G00446860 Rroxscaffold_28G00446910 Rroxscaffold_29G00441560 Rroxscaffold_29G00441570 Rroxscaffold_29G00441590 Rroxscaffold_29G00441630 Rroxscaffold_29G00441640 Rroxscaffold_29G00441670 Rroxscaffold_29G00441680 Rroxscaffold_29G00441860 Rroxscaffold_30G00449540 Rroxscaffold_30G00449570 Rroxscaffold_30G00449580 Rroxscaffold_31G00438120 Rroxscaffold_31G00438140 Rroxscaffold_31G00438180 Rroxscaffold_31G00438190 Rroxscaffold_31G00438220 Rroxscaffold_31G00438230 Rroxscaffold_32G00442740 Rroxscaffold_32G00442760 Rroxscaffold_33G00439670 Rroxscaffold_33G00439720 Rroxscaffold_33G00439740 Rroxscaffold_33G00439760 Rroxscaffold_33G00439820 Rroxscaffold_34G00443140 Rroxscaffold_34G00443200 Rroxscaffold_34G00443250 Rroxscaffold_34G00443370 Rroxscaffold_34G00443390 Rroxscaffold_35G00441050 Rroxscaffold_35G00441080 Rroxscaffold_35G00441090 Rroxscaffold_35G00441170 Rroxscaffold_35G00441190 Rroxscaffold_35G00441230 Rroxscaffold_36G00440100 Rroxscaffold_36G00440170 Rroxscaffold_37G00445170 Rroxscaffold_38G00444440 Rroxscaffold_38G00444500 Rroxscaffold_38G00444550 Rroxscaffold_39G00448200 Rroxscaffold_39G00448210 Rroxscaffold_40G00447650 Rroxscaffold_40G00447680 Rroxscaffold_40G00447700 Rroxscaffold_40G00447710 Rroxscaffold_41G00451110 Rroxscaffold_41G00451130 Rroxscaffold_42G00450430 Rroxscaffold_43G00449800 Rroxscaffold_43G00449860 Rroxscaffold_43G00449920 Rroxscaffold_43G00449930 Rroxscaffold_44G00440540 Rroxscaffold_44G00440580 Rroxscaffold_44G00440660 Rroxscaffold_45G00438900 Rroxscaffold_45G00438930 Rroxscaffold_45G00438950 Rroxscaffold_47G00444020 Rroxscaffold_47G00444060 Rroxscaffold_47G00444120 Rroxscaffold_48G00448450 Rroxscaffold_49G00438570 Rroxscaffold_49G00438650 Rroxscaffold_49G00438700 Rroxscaffold_4G00321080 Rroxscaffold_50G00444130 Rroxscaffold_50G00444200 Rroxscaffold_50G00444210 Rroxscaffold_50G00444330 Rroxscaffold_50G00444350 Rroxscaffold_51G00447840 Rroxscaffold_52G00439310 Rroxscaffold_52G00439390 Rroxscaffold_54G00443660 Rroxscaffold_55G00450020 Rroxscaffold_55G00450050 Rroxscaffold_55G00450060 Rroxscaffold_55G00450080 Rroxscaffold_55G00450090 Rroxscaffold_55G00450120 Rroxscaffold_57G00443070 Rroxscaffold_59G00442210 Rroxscaffold_59G00442220 Rroxscaffold_60G00448360 Rroxscaffold_60G00448370 Rroxscaffold_60G00448410 Rroxscaffold_61G00449990 Rroxscaffold_62G00438040 Rroxscaffold_62G00438060 Rroxscaffold_62G00438080 Rroxscaffold_63G00444410 Rroxscaffold_63G00444430 Rroxscaffold_64G00450670 Rroxscaffold_65G00445300 Rroxscaffold_65G00445340 Rroxscaffold_65G00445360 Rroxscaffold_65G00445380 Rroxscaffold_65G00445420 Rroxscaffold_66G00437660 Rroxscaffold_66G00437820 Rroxscaffold_66G00437840 Rroxscaffold_66G00437890 Rroxscaffold_67G00448140 Rroxscaffold_67G00448150 Rroxscaffold_68G00447010 Rroxscaffold_69G00446670 Rroxscaffold_69G00446710 Rroxscaffold_69G00446770 Rroxscaffold_6G00387820 Rroxscaffold_70G00446300 Rroxscaffold_70G00446340 Rroxscaffold_70G00446380 Rroxscaffold_71G00445590 Rroxscaffold_71G00445600 Rroxscaffold_71G00445630 Rroxscaffold_71G00445690 Rroxscaffold_71G00445720 Rroxscaffold_71G00445740 Rroxscaffold_71G00445770 Rroxscaffold_72G00449370 Rroxscaffold_72G00449410 Rroxscaffold_72G00449450 Rroxscaffold_73G00439000 Rroxscaffold_73G00439060 Rroxscaffold_73G00439160 Rroxscaffold_73G00439270 Rroxscaffold_74G00442920 Rroxscaffold_75G00447260 Rroxscaffold_75G00447290 Rroxscaffold_75G00447430 Rroxscaffold_76G00448540 Rroxscaffold_76G00448610 Rroxscaffold_77G00449150 Rroxscaffold_78G00449610 Rroxscaffold_79G00450210 Rroxscaffold_80G00450330 Rroxscaffold_80G00450350 Rroxscaffold_81G00450480 Rroxscaffold_83G00450980 Rroxscaffold_85G00451230 Rroxscaffold_86G00451270 Rroxscaffold_89G00451410 Rroxscaffold_90G00448760 Rroxscaffold_90G00448770 Rroxscaffold_90G00448820 Rroxscaffold_91G00443000 Rroxscaffold_91G00443060 Rroxscaffold_92G00446430 Rroxscaffold_93G00440830 Rroxscaffold_93G00440850 Rroxscaffold_93G00440860 Rroxscaffold_93G00440880 Rroxscaffold_93G00440910 Rroxscaffold_94G00445830 Rroxscaffold_94G00445910 Rroxscaffold_94G00445940 Rroxscaffold_94G00446020 Rroxscaffold_97G00442340 Rroxscaffold_97G00442390 Rroxscaffold_97G00442400 Rroxscaffold_98G00451350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 267
Acc36I ACCTGC 1 cut(s) 267
AccI GTMKAC 1 cut(s) 565
AciI CCGC 5 cut(s) 284, 319, 333, 374, 516
AclI AACGTT 1 cut(s) 404
AclWI GGATC 3 cut(s) 50, 151, 333
AcsI RAATTY 1 cut(s) 125
AfaI GTAC 1 cut(s) 178
AfiI CCNNNNNNNGG 2 cut(s) 360, 386
AjuI GAANNNNNNNTTGG 2 cut(s) 330, 362
AluBI AGCT 4 cut(s) 120, 201, 244, 495
AluI AGCT 4 cut(s) 120, 201, 244, 495
Alw21I GWGCWC 1 cut(s) 246
AlwI GGATC 3 cut(s) 50, 151, 333
AoxI GGCC 1 cut(s) 151
ApeKI GCWGC 1 cut(s) 74
ApoI RAATTY 1 cut(s) 125
AspS9I GGNCC 1 cut(s) 351
AsuC2I CCSGG 1 cut(s) 323
AvaII GGWCC 1 cut(s) 351
BaeGI GKGCMC 1 cut(s) 373
BanII GRGCYC 1 cut(s) 246
BauI CACGAG 1 cut(s) 221
BbsI GAAGAC 2 cut(s) 307, 498
Bbv12I GWGCWC 1 cut(s) 246
BbvI GCAGC 1 cut(s) 61
BcnI CCSGG 1 cut(s) 323
BfaI CTAG 2 cut(s) 117, 485
BfuAI ACCTGC 1 cut(s) 267
BisI GCNGC 2 cut(s) 75, 284
BlsI GCNGC 2 cut(s) 76, 285
Bme1390I CCNGG 1 cut(s) 323
Bme18I GGWCC 1 cut(s) 351
BmgT120I GGNCC 1 cut(s) 351
BmiI GGNNCC 2 cut(s) 352, 441
BmrFI CCNGG 1 cut(s) 323
BoxI GACNNNNGTC 1 cut(s) 487
BpiI GAAGAC 2 cut(s) 307, 498
BplI GAGNNNNNCTC 2 cut(s) 479, 511
BpuEI CTTGAG 1 cut(s) 446
BpuMI CCSGG 1 cut(s) 323
Bsa29I ATCGAT 1 cut(s) 146
BsaJI CCNNGG 1 cut(s) 540
Bsc4I CCNNNNNNNGG 2 cut(s) 360, 386
Bse1I ACTGG 1 cut(s) 184
BseCI ATCGAT 1 cut(s) 146
BseDI CCNNGG 1 cut(s) 540
BseGI GGATG 1 cut(s) 283
BseLI CCNNNNNNNGG 2 cut(s) 360, 386
BseNI ACTGG 1 cut(s) 184
BseSI GKGCMC 1 cut(s) 373
BseXI GCAGC 1 cut(s) 61
BseYI CCCAGC 1 cut(s) 288
BshFI GGCC 1 cut(s) 153
BshVI ATCGAT 1 cut(s) 146
BsiHKAI GWGCWC 1 cut(s) 246
BsiSI CCGG 1 cut(s) 323
BslFI GGGAC 1 cut(s) 585
BslI CCNNNNNNNGG 2 cut(s) 360, 386
BsmFI GGGAC 1 cut(s) 585
BsnI GGCC 1 cut(s) 153
Bsp1286I GDGCHC 2 cut(s) 246, 373
Bsp143I GATC 3 cut(s) 42, 143, 325
BspACI CCGC 5 cut(s) 284, 319, 333, 374, 516
BspANI GGCC 1 cut(s) 153
BspDI ATCGAT 1 cut(s) 146
BspLI GGNNCC 2 cut(s) 352, 441
BspMI ACCTGC 1 cut(s) 267
BspPI GGATC 3 cut(s) 50, 151, 333
BspQI GCTCTTC 1 cut(s) 20
BsrI ACTGG 1 cut(s) 184
BssECI CCNNGG 1 cut(s) 540
BssMI GATC 3 cut(s) 42, 143, 325
BssSI CACGAG 1 cut(s) 221
BssT1I CCWWGG 1 cut(s) 540
Bst2BI CACGAG 1 cut(s) 221
Bst4CI ACNGT 2 cut(s) 166, 576
Bst6I CTCTTC 1 cut(s) 20
BstC8I GCNNGC 2 cut(s) 333, 343
BstDEI CTNAG 1 cut(s) 250
BstF5I GGATG 1 cut(s) 283
BstKTI GATC 3 cut(s) 45, 146, 328
BstMBI GATC 3 cut(s) 42, 143, 325
BstMWI GCNNNNNNNGC 3 cut(s) 66, 289, 524
BstPAI GACNNNNGTC 1 cut(s) 487
BstSCI CCNGG 1 cut(s) 321
BstSLI GKGCMC 1 cut(s) 373
BstV1I GCAGC 1 cut(s) 61
BstV2I GAAGAC 2 cut(s) 307, 498
Bsu15I ATCGAT 1 cut(s) 146
BsuRI GGCC 1 cut(s) 153
BsuTUI ATCGAT 1 cut(s) 146
BtsCI GGATG 1 cut(s) 283
BtsI GCAGTG 1 cut(s) 373
BtsIMutI CAGTG 2 cut(s) 373, 581
BveI ACCTGC 1 cut(s) 267
Cac8I GCNNGC 2 cut(s) 333, 343
Cfr13I GGNCC 1 cut(s) 351
ClaI ATCGAT 1 cut(s) 146
Csp6I GTAC 1 cut(s) 177
CviQI GTAC 1 cut(s) 177
DdeI CTNAG 1 cut(s) 250
DpnI GATC 3 cut(s) 44, 145, 327
DpnII GATC 3 cut(s) 42, 143, 325
DraI TTTAAA 1 cut(s) 124
Eam1104I CTCTTC 1 cut(s) 20
EarI CTCTTC 1 cut(s) 20
EciI GGCGGA 1 cut(s) 308
Ecl136II GAGCTC 1 cut(s) 244
Eco130I CCWWGG 1 cut(s) 540
Eco24I GRGCYC 1 cut(s) 246
Eco47I GGWCC 1 cut(s) 351
Eco53kI GAGCTC 1 cut(s) 244
EcoICRI GAGCTC 1 cut(s) 244
EcoT14I CCWWGG 1 cut(s) 540
EcoT38I GRGCYC 1 cut(s) 246
ErhI CCWWGG 1 cut(s) 540
FaiI YATR 2 cut(s) 63, 450
FaqI GGGAC 1 cut(s) 585
FauI CCCGC 3 cut(s) 340, 381, 523
FblI GTMKAC 1 cut(s) 565
Fnu4HI GCNGC 2 cut(s) 75, 284
FokI GGATG 1 cut(s) 290
FriOI GRGCYC 1 cut(s) 246
Fsp4HI GCNGC 2 cut(s) 75, 284
FspBI CTAG 2 cut(s) 117, 485
GluI GCNGC 2 cut(s) 75, 284
GsaI CCCAGC 1 cut(s) 292
HaeIII GGCC 1 cut(s) 153
HapII CCGG 1 cut(s) 323
HinfI GANTC 6 cut(s) 16, 190, 382, 488, 521, 581
HpaII CCGG 1 cut(s) 323
Hpy166II GTNNAC 1 cut(s) 566
Hpy188I TCNGA 4 cut(s) 109, 189, 381, 482
Hpy8I GTNNAC 1 cut(s) 566
Hpy99I CGWCG 1 cut(s) 60
HpyAV CCTTC 2 cut(s) 33, 430
HpyCH4III ACNGT 2 cut(s) 166, 576
HpyCH4IV ACGT 2 cut(s) 55, 404
HpyF10VI GCNNNNNNNGC 3 cut(s) 66, 289, 524
HpyF3I CTNAG 1 cut(s) 250
HpySE526I ACGT 2 cut(s) 55, 404
Kzo9I GATC 3 cut(s) 42, 143, 325
LguI GCTCTTC 1 cut(s) 20
LmnI GCTCC 1 cut(s) 445
Lsp1109I GCAGC 1 cut(s) 61
MaeI CTAG 2 cut(s) 117, 485
MaeII ACGT 2 cut(s) 55, 404
MaeIII GTNAC 1 cut(s) 98
MalI GATC 3 cut(s) 44, 145, 327
MboI GATC 3 cut(s) 42, 143, 325
MboII GAAGA 3 cut(s) 37, 307, 498
MfeI CAATTG 1 cut(s) 7
MhlI GDGCHC 2 cut(s) 246, 373
MluCI AATT 2 cut(s) 7, 125
MlyI GAGTC 1 cut(s) 497
MmeI TCCRAC 1 cut(s) 460
MnlI CCTC 4 cut(s) 124, 354, 387, 468
MseI TTAA 4 cut(s) 123, 272, 407, 589
MspA1I CMGCKG 1 cut(s) 518
MspI CCGG 1 cut(s) 323
MspR9I CCNGG 1 cut(s) 323
MunI CAATTG 1 cut(s) 7
MwoI GCNNNNNNNGC 3 cut(s) 66, 289, 524
NciI CCSGG 1 cut(s) 323
NdeII GATC 3 cut(s) 42, 143, 325
NlaIV GGNNCC 2 cut(s) 352, 441
PaqCI CACCTGC 1 cut(s) 267
PciSI GCTCTTC 1 cut(s) 20
PcsI WCGNNNNNNNCGW 1 cut(s) 28
PfeI GAWTC 5 cut(s) 16, 190, 382, 521, 581
PflFI GACNNNGTC 1 cut(s) 311
PkrI GCNGC 2 cut(s) 76, 285
PleI GAGTC 1 cut(s) 496
PpsI GAGTC 1 cut(s) 496
PshAI GACNNNNGTC 1 cut(s) 487
Psp124BI GAGCTC 1 cut(s) 246
Psp1406I AACGTT 1 cut(s) 404
PspFI CCCAGC 1 cut(s) 288
PspN4I GGNNCC 2 cut(s) 352, 441
PspPI GGNCC 1 cut(s) 351
PsyI GACNNNGTC 1 cut(s) 311
RsaI GTAC 1 cut(s) 178
RsaNI GTAC 1 cut(s) 177
SacI GAGCTC 1 cut(s) 246
SapI GCTCTTC 1 cut(s) 20
SaqAI TTAA 4 cut(s) 123, 272, 407, 589
SatI GCNGC 2 cut(s) 75, 284
Sau3AI GATC 3 cut(s) 42, 143, 325
Sau96I GGNCC 1 cut(s) 351
SchI GAGTC 1 cut(s) 497
ScrFI CCNGG 1 cut(s) 323
SduI GDGCHC 2 cut(s) 246, 373
SinI GGWCC 1 cut(s) 351
SmlI CTYRAG 1 cut(s) 461
SmoI CTYRAG 1 cut(s) 461
Sse9I AATT 2 cut(s) 7, 125
SsiI CCGC 5 cut(s) 284, 319, 333, 374, 516
SspMI CTAG 2 cut(s) 117, 485
SstI GAGCTC 1 cut(s) 246
StyD4I CCNGG 1 cut(s) 321
StyI CCWWGG 1 cut(s) 540
TaaI ACNGT 2 cut(s) 166, 576
TaiI ACGT 2 cut(s) 58, 407
TaqI TCGA 2 cut(s) 37, 146
TasI AATT 2 cut(s) 7, 125
TauI GCSGC 1 cut(s) 286
TfiI GAWTC 5 cut(s) 16, 190, 382, 521, 581
Tru1I TTAA 4 cut(s) 123, 272, 407, 589
Tru9I TTAA 4 cut(s) 123, 272, 407, 589
TscAI CASTG 2 cut(s) 373, 581
TseI GCWGC 1 cut(s) 74
TspDTI ATGAA 1 cut(s) 78
TspGWI ACGGA 3 cut(s) 37, 290, 402
TspRI CASTG 2 cut(s) 373, 581
Tth111I GACNNNGTC 1 cut(s) 311
VpaK11BI GGWCC 1 cut(s) 351
XapI RAATTY 1 cut(s) 125
XcmI CCANNNNNNNNNTGG 1 cut(s) 92
XmiI GTMKAC 1 cut(s) 565
XspI CTAG 2 cut(s) 117, 485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.