Rroxscaffold_103G00450530

mitotic sister chromatid biorientation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000103
OG0000098
Physical Location & Seq
Forward (+)
17688 .. 19843
2156 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_103G00450530.1

Sequence Viewer

Length: 1407 bp
ATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCTAGCTTTAAATTCCAAAGGTCTAAAGGATCGATAGGCCACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATTTCTGTTCTCGTTGAGCTCATCTTAGGACACCTGCGTTATCTTTTAACAGATGTGCCGCCCCAGCCAAACTCCCCACCTGACAATGTCTTCCGCCCGGATCAGCCCGCCGAAGCAGGCTTTGGGTCCAAAAAGAGGGGCAGTGCCCCGCCTCCGATTCACGGAATAAGTAAAATAACGTTAAAAGTAGTGGTATTTCACTTTCGCCCGAAGGCTCCCACTTATACTACACCTCTCAAGTCATTTCACAAAGTCGGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGACAGGGACATCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTGAACGCCCGGGAAAGCCCCCGAAGGAGCGTTCCCAGTCCGTCCCCCGGCCGGCACGCGGCGACCCGCTCTCGCCGCGGAAGCAGCTCGAGCAGTTCACCGACAGCCGACGGGTTCGGGACTGGGACCCCCGTGCCCAGCCCTCGAGCCAATCCTTTTCCCGAGGTTACGGATCCATTTTGCCGACTTCCCTTGCCTACATTGTTCCATTGACCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCGTGAATGGCACTCGGTCCTCCGGATTTTCAAGGGCCGCCGGGGGCGCACCGGACACCACGCGACGTGCGGTGCTCTTCCAGCCGCTGGACCCTACCTCCGGCTGAGCCGTTTCCAGGGTGGGCAGGCTGATCGACTAACCCATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATCTGCACCGACGGCCGCTCCGCCGGGCTCACGCCCCAGGTTTTGCAGCGACCGCCGCGCCCTCCTACTCATCGGGGCCTGGCACTTGCCCCGACGGCCGGGTATAGGTCACGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGACGAACGATTTGCACGTCAGTATCGCTGCGGGCCTCCACCAGAGTTTCCTCTGGCTTCGCCCCGCTCAGGCATAGTTCACCATCTTTCGGGTCCCGACAGGCATGCTCACACTCGAACCCTTCTCAGAAGATCAAGGTCGGTCGGCGGTGCACCCGCAAAGGGATCCCGCACATTAGCTTCCTTGCGCCTTACGGGTTTAATCACCCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

468

Amino Acids

51.62

Weight (kDa)

11.34

Isoelectric Point (pI)

57.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000098)

Species Orthologous Gene IDs
pyrus_communis pycom05g23460 pycom581g00100 pycom849g00040
rosa_multiflora Rmu_sc0019961.1_g000001
rosa_roxburghii Rroxscaffold_100G00451180 Rroxscaffold_102G00450890 Rroxscaffold_102G00450920 Rroxscaffold_103G00450530 Rroxscaffold_103G00450560 Rroxscaffold_104G00444830 Rroxscaffold_104G00444900 Rroxscaffold_105G00447100 Rroxscaffold_105G00447180 Rroxscaffold_107G00442470 Rroxscaffold_107G00442480 Rroxscaffold_107G00442520 Rroxscaffold_107G00442540 Rroxscaffold_107G00442560 Rroxscaffold_107G00442570 Rroxscaffold_107G00442600 Rroxscaffold_107G00442630 Rroxscaffold_107G00442650 Rroxscaffold_107G00442660 Rroxscaffold_108G00451480 Rroxscaffold_110G00451510 Rroxscaffold_111G00451550 Rroxscaffold_112G00451590 Rroxscaffold_113G00451630 Rroxscaffold_114G00451650 Rroxscaffold_116G00451710 Rroxscaffold_117G00451740 Rroxscaffold_117G00451750 Rroxscaffold_121G00451840 Rroxscaffold_122G00451860 Rroxscaffold_122G00451880 Rroxscaffold_123G00451920 Rroxscaffold_124G00451950 Rroxscaffold_128G00452040 Rroxscaffold_128G00452060 Rroxscaffold_12G00450600 Rroxscaffold_12G00450620 Rroxscaffold_131G00452110 Rroxscaffold_138G00452180 Rroxscaffold_13G00448630 Rroxscaffold_145G00452240 Rroxscaffold_14G00441930 Rroxscaffold_14G00441950 Rroxscaffold_14G00442050 Rroxscaffold_14G00442080 Rroxscaffold_15G00447500 Rroxscaffold_162G00450770 Rroxscaffold_16G00446200 Rroxscaffold_16G00446210 Rroxscaffold_16G00446240 Rroxscaffold_17G00435360 Rroxscaffold_17G00435410 Rroxscaffold_17G00435450 Rroxscaffold_17G00435480 Rroxscaffold_17G00435500 Rroxscaffold_17G00435600 Rroxscaffold_17G00435660 Rroxscaffold_17G00435680 Rroxscaffold_17G00435760 Rroxscaffold_17G00435780 Rroxscaffold_17G00435810 Rroxscaffold_17G00435840 Rroxscaffold_17G00435870 Rroxscaffold_18G00446030 Rroxscaffold_18G00446060 Rroxscaffold_18G00446130 Rroxscaffold_19G00448890 Rroxscaffold_19G00448920 Rroxscaffold_19G00448940 Rroxscaffold_19G00448970 Rroxscaffold_1G00000020 Rroxscaffold_1G00000050 Rroxscaffold_1G00000160 Rroxscaffold_20G00445060 Rroxscaffold_20G00445100 Rroxscaffold_20G00445130 Rroxscaffold_21G00439490 Rroxscaffold_21G00439560 Rroxscaffold_21G00439580 Rroxscaffold_21G00439620 Rroxscaffold_21G00439630 Rroxscaffold_22G00439910 Rroxscaffold_22G00439950 Rroxscaffold_23G00451020 Rroxscaffold_23G00451040 Rroxscaffold_23G00451070 Rroxscaffold_24G00444920 Rroxscaffold_24G00444950 Rroxscaffold_24G00445000 Rroxscaffold_25G00450200 Rroxscaffold_26G00447730 Rroxscaffold_27G00446570 Rroxscaffold_27G00446600 Rroxscaffold_28G00446830 Rroxscaffold_28G00446860 Rroxscaffold_28G00446910 Rroxscaffold_29G00441560 Rroxscaffold_29G00441570 Rroxscaffold_29G00441590 Rroxscaffold_29G00441630 Rroxscaffold_29G00441640 Rroxscaffold_29G00441670 Rroxscaffold_29G00441680 Rroxscaffold_29G00441860 Rroxscaffold_30G00449540 Rroxscaffold_30G00449570 Rroxscaffold_30G00449580 Rroxscaffold_31G00438120 Rroxscaffold_31G00438140 Rroxscaffold_31G00438180 Rroxscaffold_31G00438190 Rroxscaffold_31G00438220 Rroxscaffold_31G00438230 Rroxscaffold_32G00442740 Rroxscaffold_32G00442760 Rroxscaffold_33G00439670 Rroxscaffold_33G00439720 Rroxscaffold_33G00439740 Rroxscaffold_33G00439760 Rroxscaffold_33G00439820 Rroxscaffold_34G00443140 Rroxscaffold_34G00443200 Rroxscaffold_34G00443250 Rroxscaffold_34G00443370 Rroxscaffold_34G00443390 Rroxscaffold_35G00441050 Rroxscaffold_35G00441080 Rroxscaffold_35G00441090 Rroxscaffold_35G00441170 Rroxscaffold_35G00441190 Rroxscaffold_35G00441230 Rroxscaffold_36G00440100 Rroxscaffold_36G00440170 Rroxscaffold_37G00445170 Rroxscaffold_38G00444440 Rroxscaffold_38G00444500 Rroxscaffold_38G00444550 Rroxscaffold_39G00448200 Rroxscaffold_39G00448210 Rroxscaffold_40G00447650 Rroxscaffold_40G00447680 Rroxscaffold_40G00447700 Rroxscaffold_40G00447710 Rroxscaffold_41G00451110 Rroxscaffold_41G00451130 Rroxscaffold_42G00450430 Rroxscaffold_43G00449800 Rroxscaffold_43G00449860 Rroxscaffold_43G00449920 Rroxscaffold_43G00449930 Rroxscaffold_44G00440540 Rroxscaffold_44G00440580 Rroxscaffold_44G00440660 Rroxscaffold_45G00438900 Rroxscaffold_45G00438930 Rroxscaffold_45G00438950 Rroxscaffold_47G00444020 Rroxscaffold_47G00444060 Rroxscaffold_47G00444120 Rroxscaffold_48G00448450 Rroxscaffold_49G00438570 Rroxscaffold_49G00438650 Rroxscaffold_49G00438700 Rroxscaffold_4G00321080 Rroxscaffold_50G00444130 Rroxscaffold_50G00444200 Rroxscaffold_50G00444210 Rroxscaffold_50G00444330 Rroxscaffold_50G00444350 Rroxscaffold_51G00447840 Rroxscaffold_52G00439310 Rroxscaffold_52G00439390 Rroxscaffold_54G00443660 Rroxscaffold_55G00450020 Rroxscaffold_55G00450050 Rroxscaffold_55G00450060 Rroxscaffold_55G00450080 Rroxscaffold_55G00450090 Rroxscaffold_55G00450120 Rroxscaffold_57G00443070 Rroxscaffold_59G00442210 Rroxscaffold_59G00442220 Rroxscaffold_60G00448360 Rroxscaffold_60G00448370 Rroxscaffold_60G00448410 Rroxscaffold_61G00449990 Rroxscaffold_62G00438040 Rroxscaffold_62G00438060 Rroxscaffold_62G00438080 Rroxscaffold_63G00444410 Rroxscaffold_63G00444430 Rroxscaffold_64G00450670 Rroxscaffold_65G00445300 Rroxscaffold_65G00445340 Rroxscaffold_65G00445360 Rroxscaffold_65G00445380 Rroxscaffold_65G00445420 Rroxscaffold_66G00437660 Rroxscaffold_66G00437820 Rroxscaffold_66G00437840 Rroxscaffold_66G00437890 Rroxscaffold_67G00448140 Rroxscaffold_67G00448150 Rroxscaffold_68G00447010 Rroxscaffold_69G00446670 Rroxscaffold_69G00446710 Rroxscaffold_69G00446770 Rroxscaffold_6G00387820 Rroxscaffold_70G00446300 Rroxscaffold_70G00446340 Rroxscaffold_70G00446380 Rroxscaffold_71G00445590 Rroxscaffold_71G00445600 Rroxscaffold_71G00445630 Rroxscaffold_71G00445690 Rroxscaffold_71G00445720 Rroxscaffold_71G00445740 Rroxscaffold_71G00445770 Rroxscaffold_72G00449370 Rroxscaffold_72G00449410 Rroxscaffold_72G00449450 Rroxscaffold_73G00439000 Rroxscaffold_73G00439060 Rroxscaffold_73G00439160 Rroxscaffold_73G00439270 Rroxscaffold_74G00442920 Rroxscaffold_75G00447260 Rroxscaffold_75G00447290 Rroxscaffold_75G00447430 Rroxscaffold_76G00448540 Rroxscaffold_76G00448610 Rroxscaffold_77G00449150 Rroxscaffold_78G00449610 Rroxscaffold_79G00450210 Rroxscaffold_80G00450330 Rroxscaffold_80G00450350 Rroxscaffold_81G00450480 Rroxscaffold_83G00450980 Rroxscaffold_85G00451230 Rroxscaffold_86G00451270 Rroxscaffold_89G00451410 Rroxscaffold_90G00448760 Rroxscaffold_90G00448770 Rroxscaffold_90G00448820 Rroxscaffold_91G00443000 Rroxscaffold_91G00443060 Rroxscaffold_92G00446430 Rroxscaffold_93G00440830 Rroxscaffold_93G00440850 Rroxscaffold_93G00440860 Rroxscaffold_93G00440880 Rroxscaffold_93G00440910 Rroxscaffold_94G00445830 Rroxscaffold_94G00445910 Rroxscaffold_94G00445940 Rroxscaffold_94G00446020 Rroxscaffold_97G00442340 Rroxscaffold_97G00442390 Rroxscaffold_97G00442400 Rroxscaffold_98G00451350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 207
Acc36I ACCTGC 1 cut(s) 207
AccB7I CCANNNNNTGG 1 cut(s) 902
AccBSI CCGCTC 3 cut(s) 622, 1058, 1260
AccI GTMKAC 2 cut(s) 505, 548
AccII CGCG 5 cut(s) 612, 631, 878, 1098, 1154
AccIII TCCGGA 1 cut(s) 837
AclI AACGTT 1 cut(s) 344
AclWI GGATC 6 cut(s) 91, 273, 720, 733, 1353, 1366
AcoI YGGCCR 3 cut(s) 602, 1053, 1136
AcsI RAATTY 1 cut(s) 65
AfaI GTAC 3 cut(s) 118, 535, 808
AflII CTTAAG 1 cut(s) 1157
AgsI TTSAA 4 cut(s) 557, 847, 1005, 1019
AjiI CACGTC 2 cut(s) 882, 1211
AjnI CCWGG 3 cut(s) 930, 1076, 1118
AjuI GAANNNNNNNTTGG 2 cut(s) 270, 302
AluBI AGCT 6 cut(s) 60, 141, 184, 435, 640, 1373
AluI AGCT 6 cut(s) 60, 141, 184, 435, 640, 1373
Alw21I GWGCWC 3 cut(s) 186, 892, 1348
Alw26I GTCTC 1 cut(s) 781
Alw44I GTGCAC 1 cut(s) 1344
AlwI GGATC 6 cut(s) 91, 273, 720, 733, 1353, 1366
AlwNI CAGNNNCTG 2 cut(s) 774, 902
Ama87I CYCGRG 4 cut(s) 562, 641, 697, 714
Aor13HI TCCGGA 1 cut(s) 837
AoxI GGCC 8 cut(s) 91, 602, 850, 998, 1053, 1116, 1136, 1226
ApaLI GTGCAC 1 cut(s) 1344
ApeKI GCWGC 4 cut(s) 13, 637, 1086, 1221
ApoI RAATTY 1 cut(s) 65
ArsI GACNNNNNNTTYG 2 cut(s) 1187, 1219
AspLEI GCGC 5 cut(s) 864, 1100, 1156, 1164, 1383
AspS9I GGNCC 8 cut(s) 291, 679, 832, 850, 905, 1116, 1226, 1286
AsuC2I CCSGG 8 cut(s) 263, 563, 564, 601, 814, 857, 1065, 1140
AsuHPI GGTGA 4 cut(s) 643, 771, 1265, 1390
AvaI CYCGRG 4 cut(s) 562, 641, 697, 714
AvaII GGWCC 5 cut(s) 291, 679, 832, 905, 1286
BaeGI GKGCMC 3 cut(s) 313, 691, 1348
BaeI ACNNNNGTAYC 2 cut(s) 1199, 1232
BamHI GGATCC 2 cut(s) 725, 1358
BanII GRGCYC 2 cut(s) 186, 1070
BauI CACGAG 1 cut(s) 161
BbsI GAAGAC 2 cut(s) 247, 438
Bbv12I GWGCWC 3 cut(s) 186, 892, 1348
BbvI GCAGC 3 cut(s) 649, 1098, 1208
BccI CCATC 2 cut(s) 1173, 1284
BceAI ACGGC 4 cut(s) 909, 955, 1068, 1151
BcgI CGANNNNNNTGC 6 cut(s) 929, 963, 1187, 1221, 1280, 1314
BciT130I CCWGG 3 cut(s) 932, 1078, 1120
BcnI CCSGG 8 cut(s) 263, 563, 564, 601, 814, 857, 1065, 1140
BcoDI GTCTC 1 cut(s) 781
BfaI CTAG 3 cut(s) 57, 425, 1181
BfoI RGCGCY 1 cut(s) 1165
BfrI CTTAAG 1 cut(s) 1157
BfuAI ACCTGC 1 cut(s) 207
BglI GCCNNNNNGGC 1 cut(s) 1135
BglII AGATCT 1 cut(s) 1040
BlpI GCTNAGC 1 cut(s) 920
Bme18I GGWCC 5 cut(s) 291, 679, 832, 905, 1286
BmeT110I CYCGRG 4 cut(s) 562, 641, 697, 714
BmgBI CACGTC 2 cut(s) 882, 1211
BmgT120I GGNCC 8 cut(s) 291, 679, 832, 850, 905, 1116, 1226, 1286
BmrI ACTGGG 2 cut(s) 583, 685
BmsI GCATC 1 cut(s) 784
BmuI ACTGGG 2 cut(s) 583, 685
BoxI GACNNNNGTC 1 cut(s) 427
BpiI GAAGAC 2 cut(s) 247, 438
BplI GAGNNNNNCTC 2 cut(s) 419, 451
Bpu10I CCTNAGC 1 cut(s) 1261
Bpu1102I GCTNAGC 1 cut(s) 920
BpuEI CTTGAG 1 cut(s) 386
BpuMI CCSGG 8 cut(s) 263, 563, 564, 601, 814, 857, 1065, 1140
Bsa29I ATCGAT 1 cut(s) 86
BsaI GGTCTC 1 cut(s) 781
BsaJI CCNNGG 9 cut(s) 480, 562, 599, 629, 715, 782, 856, 931, 1076
BsaWI WCCGGW 2 cut(s) 837, 866
BsaXI ACNNNNNCTCC 4 cut(s) 897, 927, 1042, 1072
Bse118I RCCGGY 1 cut(s) 604
Bse1I ACTGG 5 cut(s) 24, 124, 537, 589, 680
BseAI TCCGGA 1 cut(s) 837
BseBI CCWGG 3 cut(s) 932, 1078, 1120
BseCI ATCGAT 1 cut(s) 86
BseDI CCNNGG 9 cut(s) 480, 562, 599, 629, 715, 782, 856, 931, 1076
BseGI GGATG 1 cut(s) 1165
BseMII CTCAG 4 cut(s) 534, 911, 1275, 1333
BseNI ACTGG 5 cut(s) 24, 124, 537, 589, 680
BseSI GKGCMC 3 cut(s) 313, 691, 1348
BseX3I CGGCCG 3 cut(s) 602, 1053, 1136
BseXI GCAGC 3 cut(s) 649, 1098, 1208
BseYI CCCAGC 2 cut(s) 228, 690
BsgI GTGCAG 1 cut(s) 1029
Bsh1236I CGCG 5 cut(s) 612, 631, 878, 1098, 1154
Bsh1285I CGRYCG 6 cut(s) 605, 813, 1056, 1093, 1139, 1338
BshFI GGCC 8 cut(s) 93, 604, 852, 1000, 1055, 1118, 1138, 1228
BshVI ATCGAT 1 cut(s) 86
BsiEI CGRYCG 6 cut(s) 605, 813, 1056, 1093, 1139, 1338
BsiHKAI GWGCWC 3 cut(s) 186, 892, 1348
BsiHKCI CYCGRG 4 cut(s) 562, 641, 697, 714
BslFI GGGAC 5 cut(s) 525, 581, 686, 692, 1272
BsmAI GTCTC 1 cut(s) 781
BsmFI GGGAC 5 cut(s) 525, 581, 686, 692, 1272
BsnI GGCC 8 cut(s) 93, 604, 852, 1000, 1055, 1118, 1138, 1228
Bso31I GGTCTC 1 cut(s) 781
BsoBI CYCGRG 4 cut(s) 562, 641, 697, 714
Bsp1286I GDGCHC 6 cut(s) 186, 313, 691, 892, 1070, 1348
Bsp13I TCCGGA 1 cut(s) 837
Bsp143I GATC 7 cut(s) 83, 265, 725, 946, 1040, 1325, 1358
Bsp1720I GCTNAGC 1 cut(s) 920
BspANI GGCC 8 cut(s) 93, 604, 852, 1000, 1055, 1118, 1138, 1228
BspCNI CTCAG 4 cut(s) 535, 912, 1274, 1332
BspDI ATCGAT 1 cut(s) 86
BspEI TCCGGA 1 cut(s) 837
BspFNI CGCG 5 cut(s) 612, 631, 878, 1098, 1154
BspMI ACCTGC 1 cut(s) 207
BspPI GGATC 6 cut(s) 91, 273, 720, 733, 1353, 1366
BspQI GCTCTTC 1 cut(s) 897
BspTI CTTAAG 1 cut(s) 1157
BspTNI GGTCTC 1 cut(s) 781
BsrBI CCGCTC 3 cut(s) 622, 1058, 1260
BsrFI RCCGGY 1 cut(s) 604
BsrI ACTGG 5 cut(s) 24, 124, 537, 589, 680
BssAI RCCGGY 1 cut(s) 604
BssECI CCNNGG 9 cut(s) 480, 562, 599, 629, 715, 782, 856, 931, 1076
BssMI GATC 7 cut(s) 83, 265, 725, 946, 1040, 1325, 1358
BssSI CACGAG 1 cut(s) 161
BssT1I CCWWGG 2 cut(s) 480, 782
Bst2BI CACGAG 1 cut(s) 161
Bst2UI CCWGG 3 cut(s) 932, 1078, 1120
Bst4CI ACNGT 2 cut(s) 106, 553
Bst6I CTCTTC 2 cut(s) 897, 999
BstAFI CTTAAG 1 cut(s) 1157
BstC8I GCNNGC 7 cut(s) 273, 283, 606, 610, 942, 1226, 1299
BstDEI CTNAG 5 cut(s) 190, 543, 920, 1261, 1319
BstDSI CCRYGG 1 cut(s) 629
BstF5I GGATG 1 cut(s) 1165
BstFNI CGCG 5 cut(s) 612, 631, 878, 1098, 1154
BstH2I RGCGCY 1 cut(s) 1165
BstHHI GCGC 5 cut(s) 864, 1100, 1156, 1164, 1383
BstKTI GATC 7 cut(s) 86, 268, 728, 949, 1043, 1328, 1361
BstMAI GTCTC 1 cut(s) 781
BstMBI GATC 7 cut(s) 83, 265, 725, 946, 1040, 1325, 1358
BstMCI CGRYCG 6 cut(s) 605, 813, 1056, 1093, 1139, 1338
BstNI CCWGG 3 cut(s) 932, 1078, 1120
BstNSI RCATGY 1 cut(s) 1301
BstPAI GACNNNNGTC 1 cut(s) 427
BstSLI GKGCMC 3 cut(s) 313, 691, 1348
BstUI CGCG 5 cut(s) 612, 631, 878, 1098, 1154
BstV1I GCAGC 3 cut(s) 649, 1098, 1208
BstV2I GAAGAC 2 cut(s) 247, 438
BstX2I RGATCY 3 cut(s) 725, 1040, 1358
BstYI RGATCY 3 cut(s) 725, 1040, 1358
BstZI CGGCCG 3 cut(s) 602, 1053, 1136
Bsu15I ATCGAT 1 cut(s) 86
BsuRI GGCC 8 cut(s) 93, 604, 852, 1000, 1055, 1118, 1138, 1228
BsuTUI ATCGAT 1 cut(s) 86
BtgI CCRYGG 1 cut(s) 629
BtrI CACGTC 2 cut(s) 882, 1211
BtsCI GGATG 1 cut(s) 1165
BtsI GCAGTG 1 cut(s) 313
BtsIMutI CAGTG 1 cut(s) 313
BveI ACCTGC 1 cut(s) 207
Cac8I GCNNGC 7 cut(s) 273, 283, 606, 610, 942, 1226, 1299
CaiI CAGNNNCTG 2 cut(s) 774, 902
CfoI GCGC 5 cut(s) 864, 1100, 1156, 1164, 1383
Cfr10I RCCGGY 1 cut(s) 604
Cfr13I GGNCC 8 cut(s) 291, 679, 832, 850, 905, 1116, 1226, 1286
Cfr42I CCGCGG 1 cut(s) 632
Cfr9I CCCGGG 1 cut(s) 562
ClaI ATCGAT 1 cut(s) 86
Csp6I GTAC 3 cut(s) 117, 534, 807
CviAII CATG 3 cut(s) 959, 978, 1298
CviQI GTAC 3 cut(s) 117, 534, 807
DdeI CTNAG 5 cut(s) 190, 543, 920, 1261, 1319
DpnI GATC 7 cut(s) 85, 267, 727, 948, 1042, 1327, 1360
DpnII GATC 7 cut(s) 83, 265, 725, 946, 1040, 1325, 1358
DraI TTTAAA 1 cut(s) 64
EaeI YGGCCR 3 cut(s) 602, 1053, 1136
EagI CGGCCG 3 cut(s) 602, 1053, 1136
Eam1104I CTCTTC 2 cut(s) 897, 999
EarI CTCTTC 2 cut(s) 897, 999
EciI GGCGGA 2 cut(s) 248, 1050
Ecl136II GAGCTC 1 cut(s) 184
EclXI CGGCCG 3 cut(s) 602, 1053, 1136
Eco130I CCWWGG 2 cut(s) 480, 782
Eco24I GRGCYC 2 cut(s) 186, 1070
Eco31I GGTCTC 1 cut(s) 781
Eco47I GGWCC 5 cut(s) 291, 679, 832, 905, 1286
Eco52I CGGCCG 3 cut(s) 602, 1053, 1136
Eco53kI GAGCTC 1 cut(s) 184
Eco88I CYCGRG 4 cut(s) 562, 641, 697, 714
EcoICRI GAGCTC 1 cut(s) 184
EcoO109I RGGNCCY 3 cut(s) 679, 1116, 1286
EcoRII CCWGG 3 cut(s) 930, 1076, 1118
EcoT14I CCWWGG 2 cut(s) 480, 782
EcoT38I GRGCYC 2 cut(s) 186, 1070
ErhI CCWWGG 2 cut(s) 480, 782
FaeI CATG 3 cut(s) 962, 981, 1301
FaiI YATR 7 cut(s) 390, 803, 960, 979, 1145, 1268, 1299
FaqI GGGAC 5 cut(s) 525, 581, 686, 692, 1272
FatI CATG 3 cut(s) 958, 977, 1297
FauI CCCGC 8 cut(s) 280, 321, 463, 627, 1217, 1265, 1357, 1370
FblI GTMKAC 2 cut(s) 505, 548
FokI GGATG 1 cut(s) 1152
FriOI GRGCYC 2 cut(s) 186, 1070
FspBI CTAG 3 cut(s) 57, 425, 1181
GlaI GCGC 5 cut(s) 863, 1099, 1155, 1163, 1382
GsaI CCCAGC 2 cut(s) 232, 694
HaeII RGCGCY 1 cut(s) 1165
HaeIII GGCC 8 cut(s) 93, 604, 852, 1000, 1055, 1118, 1138, 1228
HhaI GCGC 5 cut(s) 864, 1100, 1156, 1164, 1383
Hin1II CATG 3 cut(s) 962, 981, 1301
Hin6I GCGC 5 cut(s) 862, 1098, 1154, 1162, 1381
HinP1I GCGC 5 cut(s) 862, 1098, 1154, 1162, 1381
HincII GTYRAC 1 cut(s) 549
HindII GTYRAC 1 cut(s) 549
HinfI GANTC 7 cut(s) 130, 322, 428, 461, 520, 545, 1187
HphI GGTGA 4 cut(s) 643, 771, 1265, 1390
Hpy166II GTNNAC 7 cut(s) 506, 549, 651, 779, 975, 1273, 1346
Hpy188I TCNGA 7 cut(s) 49, 129, 321, 422, 519, 544, 1322
Hpy188III TCNNGA 4 cut(s) 671, 714, 838, 1289
Hpy8I GTNNAC 7 cut(s) 506, 549, 651, 779, 975, 1273, 1346
Hpy99I CGWCG 4 cut(s) 666, 883, 1054, 1137
HpyAV CCTTC 4 cut(s) 370, 571, 1011, 1325
HpyCH4III ACNGT 2 cut(s) 106, 553
HpyCH4IV ACGT 3 cut(s) 344, 881, 1210
HpyCH4V TGCA 5 cut(s) 964, 1046, 1086, 1208, 1346
HpyF3I CTNAG 5 cut(s) 190, 543, 920, 1261, 1319
HpySE526I ACGT 3 cut(s) 344, 881, 1210
Hsp92II CATG 3 cut(s) 962, 981, 1301
HspAI GCGC 5 cut(s) 862, 1098, 1154, 1162, 1381
KflI GGGWCCC 2 cut(s) 679, 1286
Kpn2I TCCGGA 1 cut(s) 837
KroI GCCGGC 1 cut(s) 604
KroNI GCCGGC 1 cut(s) 606
KspI CCGCGG 1 cut(s) 632
Kzo9I GATC 7 cut(s) 83, 265, 725, 946, 1040, 1325, 1358
LguI GCTCTTC 1 cut(s) 897
LmnI GCTCC 3 cut(s) 385, 580, 1063
Lsp1109I GCAGC 3 cut(s) 649, 1098, 1208
LweI GCATC 1 cut(s) 784
MaeI CTAG 3 cut(s) 57, 425, 1181
MaeII ACGT 3 cut(s) 344, 881, 1210
MaeIII GTNAC 3 cut(s) 38, 719, 1148
MalI GATC 7 cut(s) 85, 267, 727, 948, 1042, 1327, 1360
MbiI CCGCTC 3 cut(s) 622, 1058, 1260
MboI GATC 7 cut(s) 83, 265, 725, 946, 1040, 1325, 1358
MboII GAAGA 5 cut(s) 247, 438, 884, 986, 1335
MflI RGATCY 3 cut(s) 725, 1040, 1358
MhlI GDGCHC 6 cut(s) 186, 313, 691, 892, 1070, 1348
MluCI AATT 1 cut(s) 65
MlyI GAGTC 2 cut(s) 437, 554
MmeI TCCRAC 1 cut(s) 400
MroI TCCGGA 1 cut(s) 837
MroNI GCCGGC 1 cut(s) 604
MseI TTAA 5 cut(s) 63, 212, 347, 1158, 1394
MspA1I CMGCKG 3 cut(s) 458, 631, 902
MspCI CTTAAG 1 cut(s) 1157
MvaI CCWGG 3 cut(s) 932, 1078, 1120
MvnI CGCG 5 cut(s) 612, 631, 878, 1098, 1154
NaeI GCCGGC 1 cut(s) 606
NciI CCSGG 8 cut(s) 263, 563, 564, 601, 814, 857, 1065, 1140
NdeII GATC 7 cut(s) 83, 265, 725, 946, 1040, 1325, 1358
NgoMIV GCCGGC 1 cut(s) 604
NlaIII CATG 3 cut(s) 962, 981, 1301
NmuCI GTSAC 1 cut(s) 1148
NspI RCATGY 1 cut(s) 1301
PaeI GCATGC 1 cut(s) 1301
PaeR7I CTCGAG 2 cut(s) 641, 697
PaqCI CACCTGC 1 cut(s) 207
PciSI GCTCTTC 1 cut(s) 897
PcsI WCGNNNNNNNCGW 1 cut(s) 1207
PdiI GCCGGC 1 cut(s) 606
PfeI GAWTC 5 cut(s) 130, 322, 461, 520, 1187
PflFI GACNNNGTC 1 cut(s) 251
PflMI CCANNNNNTGG 1 cut(s) 902
PleI GAGTC 2 cut(s) 436, 553
PpsI GAGTC 2 cut(s) 436, 553
PpuMI RGGWCCY 2 cut(s) 679, 1286
PshAI GACNNNNGTC 1 cut(s) 427
Psp124BI GAGCTC 1 cut(s) 186
Psp1406I AACGTT 1 cut(s) 344
Psp5II RGGWCCY 2 cut(s) 679, 1286
Psp6I CCWGG 3 cut(s) 930, 1076, 1118
PspFI CCCAGC 2 cut(s) 228, 690
PspGI CCWGG 3 cut(s) 930, 1076, 1118
PspPI GGNCC 8 cut(s) 291, 679, 832, 850, 905, 1116, 1226, 1286
PspPPI RGGWCCY 2 cut(s) 679, 1286
PspXI VCTCGAGB 2 cut(s) 641, 697
PstNI CAGNNNCTG 2 cut(s) 774, 902
PsuI RGATCY 3 cut(s) 725, 1040, 1358
PsyI GACNNNGTC 1 cut(s) 251
RsaI GTAC 3 cut(s) 118, 535, 808
RsaNI GTAC 3 cut(s) 117, 534, 807
SacI GAGCTC 1 cut(s) 186
SacII CCGCGG 1 cut(s) 632
SalI GTCGAC 1 cut(s) 547
SapI GCTCTTC 1 cut(s) 897
SaqAI TTAA 5 cut(s) 63, 212, 347, 1158, 1394
Sau3AI GATC 7 cut(s) 83, 265, 725, 946, 1040, 1325, 1358
Sau96I GGNCC 8 cut(s) 291, 679, 832, 850, 905, 1116, 1226, 1286
SchI GAGTC 2 cut(s) 437, 554
SduI GDGCHC 6 cut(s) 186, 313, 691, 892, 1070, 1348
SfaNI GCATC 1 cut(s) 784
Sfr274I CTCGAG 2 cut(s) 641, 697
Sfr303I CCGCGG 1 cut(s) 632
SgrBI CCGCGG 1 cut(s) 632
SinI GGWCC 5 cut(s) 291, 679, 832, 905, 1286
SlaI CTCGAG 2 cut(s) 641, 697
SmaI CCCGGG 1 cut(s) 564
SmlI CTYRAG 4 cut(s) 401, 641, 697, 1157
SmoI CTYRAG 4 cut(s) 401, 641, 697, 1157
SphI GCATGC 1 cut(s) 1301
Sse9I AATT 1 cut(s) 65
SspI AATATT 1 cut(s) 1022
SspMI CTAG 3 cut(s) 57, 425, 1181
SstI GAGCTC 1 cut(s) 186
StyI CCWWGG 2 cut(s) 480, 782
TaaI ACNGT 2 cut(s) 106, 553
TaiI ACGT 3 cut(s) 347, 884, 1213
TaqI TCGA 6 cut(s) 86, 548, 642, 698, 949, 1309
TaqII GACCGA 2 cut(s) 820, 1324
TasI AATT 1 cut(s) 65
TauI GCSGC 7 cut(s) 226, 615, 631, 855, 902, 1058, 1098
TfiI GAWTC 5 cut(s) 130, 322, 461, 520, 1187
Tru1I TTAA 5 cut(s) 63, 212, 347, 1158, 1394
Tru9I TTAA 5 cut(s) 63, 212, 347, 1158, 1394
TscAI CASTG 1 cut(s) 313
TseFI GTSAC 1 cut(s) 1148
TseI GCWGC 4 cut(s) 13, 637, 1086, 1221
Tsp45I GTSAC 1 cut(s) 1148
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 4 cut(s) 342, 521, 583, 738
TspMI CCCGGG 1 cut(s) 562
TspRI CASTG 1 cut(s) 313
Tth111I GACNNNGTC 1 cut(s) 251
Van91I CCANNNNNTGG 1 cut(s) 902
Vha464I CTTAAG 1 cut(s) 1157
VneI GTGCAC 1 cut(s) 1344
VpaK11BI GGWCC 5 cut(s) 291, 679, 832, 905, 1286
XapI RAATTY 1 cut(s) 65
XceI RCATGY 1 cut(s) 1301
XhoI CTCGAG 2 cut(s) 641, 697
XmaI CCCGGG 1 cut(s) 562
XmiI GTMKAC 2 cut(s) 505, 548
XspI CTAG 3 cut(s) 57, 425, 1181
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.