Rroxscaffold_67G00448150

mitotic sister chromatid biorientation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000067
OG0000098
Physical Location & Seq
Forward (+)
52833 .. 53353
521 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_67G00448150.1

Sequence Viewer

Length: 255 bp
ATGAGTACGACCGGGCGTGAATGGCACTCGGTCCTCCGGATTTTCAAGGGCCGCCGGGGCGCGCACCGGACACCACGCGACGTGCGGTGCTCTTCCAGCCGCTGGACCCTACCTCCGGCTGAGCCGTTTCCAGGATCTGCACCGACGGCCGCTCCGCCCGGGCTCACGCCCCAGGTTTTGCAGCGACCGCCGCGCCCTCCTACTCATCGGGGCCTGGCACTTGCCCCGACGGCCGGGTATAGGTCACGCGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

84

Amino Acids

9.19

Weight (kDa)

12.0

Isoelectric Point (pI)

69.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000098)

Species Orthologous Gene IDs
pyrus_communis pycom05g23460 pycom581g00100 pycom849g00040
rosa_multiflora Rmu_sc0019961.1_g000001
rosa_roxburghii Rroxscaffold_100G00451180 Rroxscaffold_102G00450890 Rroxscaffold_102G00450920 Rroxscaffold_103G00450530 Rroxscaffold_103G00450560 Rroxscaffold_104G00444830 Rroxscaffold_104G00444900 Rroxscaffold_105G00447100 Rroxscaffold_105G00447180 Rroxscaffold_107G00442470 Rroxscaffold_107G00442480 Rroxscaffold_107G00442520 Rroxscaffold_107G00442540 Rroxscaffold_107G00442560 Rroxscaffold_107G00442570 Rroxscaffold_107G00442600 Rroxscaffold_107G00442630 Rroxscaffold_107G00442650 Rroxscaffold_107G00442660 Rroxscaffold_108G00451480 Rroxscaffold_110G00451510 Rroxscaffold_111G00451550 Rroxscaffold_112G00451590 Rroxscaffold_113G00451630 Rroxscaffold_114G00451650 Rroxscaffold_116G00451710 Rroxscaffold_117G00451740 Rroxscaffold_117G00451750 Rroxscaffold_121G00451840 Rroxscaffold_122G00451860 Rroxscaffold_122G00451880 Rroxscaffold_123G00451920 Rroxscaffold_124G00451950 Rroxscaffold_128G00452040 Rroxscaffold_128G00452060 Rroxscaffold_12G00450600 Rroxscaffold_12G00450620 Rroxscaffold_131G00452110 Rroxscaffold_138G00452180 Rroxscaffold_13G00448630 Rroxscaffold_145G00452240 Rroxscaffold_14G00441930 Rroxscaffold_14G00441950 Rroxscaffold_14G00442050 Rroxscaffold_14G00442080 Rroxscaffold_15G00447500 Rroxscaffold_162G00450770 Rroxscaffold_16G00446200 Rroxscaffold_16G00446210 Rroxscaffold_16G00446240 Rroxscaffold_17G00435360 Rroxscaffold_17G00435410 Rroxscaffold_17G00435450 Rroxscaffold_17G00435480 Rroxscaffold_17G00435500 Rroxscaffold_17G00435600 Rroxscaffold_17G00435660 Rroxscaffold_17G00435680 Rroxscaffold_17G00435760 Rroxscaffold_17G00435780 Rroxscaffold_17G00435810 Rroxscaffold_17G00435840 Rroxscaffold_17G00435870 Rroxscaffold_18G00446030 Rroxscaffold_18G00446060 Rroxscaffold_18G00446130 Rroxscaffold_19G00448890 Rroxscaffold_19G00448920 Rroxscaffold_19G00448940 Rroxscaffold_19G00448970 Rroxscaffold_1G00000020 Rroxscaffold_1G00000050 Rroxscaffold_1G00000160 Rroxscaffold_20G00445060 Rroxscaffold_20G00445100 Rroxscaffold_20G00445130 Rroxscaffold_21G00439490 Rroxscaffold_21G00439560 Rroxscaffold_21G00439580 Rroxscaffold_21G00439620 Rroxscaffold_21G00439630 Rroxscaffold_22G00439910 Rroxscaffold_22G00439950 Rroxscaffold_23G00451020 Rroxscaffold_23G00451040 Rroxscaffold_23G00451070 Rroxscaffold_24G00444920 Rroxscaffold_24G00444950 Rroxscaffold_24G00445000 Rroxscaffold_25G00450200 Rroxscaffold_26G00447730 Rroxscaffold_27G00446570 Rroxscaffold_27G00446600 Rroxscaffold_28G00446830 Rroxscaffold_28G00446860 Rroxscaffold_28G00446910 Rroxscaffold_29G00441560 Rroxscaffold_29G00441570 Rroxscaffold_29G00441590 Rroxscaffold_29G00441630 Rroxscaffold_29G00441640 Rroxscaffold_29G00441670 Rroxscaffold_29G00441680 Rroxscaffold_29G00441860 Rroxscaffold_30G00449540 Rroxscaffold_30G00449570 Rroxscaffold_30G00449580 Rroxscaffold_31G00438120 Rroxscaffold_31G00438140 Rroxscaffold_31G00438180 Rroxscaffold_31G00438190 Rroxscaffold_31G00438220 Rroxscaffold_31G00438230 Rroxscaffold_32G00442740 Rroxscaffold_32G00442760 Rroxscaffold_33G00439670 Rroxscaffold_33G00439720 Rroxscaffold_33G00439740 Rroxscaffold_33G00439760 Rroxscaffold_33G00439820 Rroxscaffold_34G00443140 Rroxscaffold_34G00443200 Rroxscaffold_34G00443250 Rroxscaffold_34G00443370 Rroxscaffold_34G00443390 Rroxscaffold_35G00441050 Rroxscaffold_35G00441080 Rroxscaffold_35G00441090 Rroxscaffold_35G00441170 Rroxscaffold_35G00441190 Rroxscaffold_35G00441230 Rroxscaffold_36G00440100 Rroxscaffold_36G00440170 Rroxscaffold_37G00445170 Rroxscaffold_38G00444440 Rroxscaffold_38G00444500 Rroxscaffold_38G00444550 Rroxscaffold_39G00448200 Rroxscaffold_39G00448210 Rroxscaffold_40G00447650 Rroxscaffold_40G00447680 Rroxscaffold_40G00447700 Rroxscaffold_40G00447710 Rroxscaffold_41G00451110 Rroxscaffold_41G00451130 Rroxscaffold_42G00450430 Rroxscaffold_43G00449800 Rroxscaffold_43G00449860 Rroxscaffold_43G00449920 Rroxscaffold_43G00449930 Rroxscaffold_44G00440540 Rroxscaffold_44G00440580 Rroxscaffold_44G00440660 Rroxscaffold_45G00438900 Rroxscaffold_45G00438930 Rroxscaffold_45G00438950 Rroxscaffold_47G00444020 Rroxscaffold_47G00444060 Rroxscaffold_47G00444120 Rroxscaffold_48G00448450 Rroxscaffold_49G00438570 Rroxscaffold_49G00438650 Rroxscaffold_49G00438700 Rroxscaffold_4G00321080 Rroxscaffold_50G00444130 Rroxscaffold_50G00444200 Rroxscaffold_50G00444210 Rroxscaffold_50G00444330 Rroxscaffold_50G00444350 Rroxscaffold_51G00447840 Rroxscaffold_52G00439310 Rroxscaffold_52G00439390 Rroxscaffold_54G00443660 Rroxscaffold_55G00450020 Rroxscaffold_55G00450050 Rroxscaffold_55G00450060 Rroxscaffold_55G00450080 Rroxscaffold_55G00450090 Rroxscaffold_55G00450120 Rroxscaffold_57G00443070 Rroxscaffold_59G00442210 Rroxscaffold_59G00442220 Rroxscaffold_60G00448360 Rroxscaffold_60G00448370 Rroxscaffold_60G00448410 Rroxscaffold_61G00449990 Rroxscaffold_62G00438040 Rroxscaffold_62G00438060 Rroxscaffold_62G00438080 Rroxscaffold_63G00444410 Rroxscaffold_63G00444430 Rroxscaffold_64G00450670 Rroxscaffold_65G00445300 Rroxscaffold_65G00445340 Rroxscaffold_65G00445360 Rroxscaffold_65G00445380 Rroxscaffold_65G00445420 Rroxscaffold_66G00437660 Rroxscaffold_66G00437820 Rroxscaffold_66G00437840 Rroxscaffold_66G00437890 Rroxscaffold_67G00448140 Rroxscaffold_67G00448150 Rroxscaffold_68G00447010 Rroxscaffold_69G00446670 Rroxscaffold_69G00446710 Rroxscaffold_69G00446770 Rroxscaffold_6G00387820 Rroxscaffold_70G00446300 Rroxscaffold_70G00446340 Rroxscaffold_70G00446380 Rroxscaffold_71G00445590 Rroxscaffold_71G00445600 Rroxscaffold_71G00445630 Rroxscaffold_71G00445690 Rroxscaffold_71G00445720 Rroxscaffold_71G00445740 Rroxscaffold_71G00445770 Rroxscaffold_72G00449370 Rroxscaffold_72G00449410 Rroxscaffold_72G00449450 Rroxscaffold_73G00439000 Rroxscaffold_73G00439060 Rroxscaffold_73G00439160 Rroxscaffold_73G00439270 Rroxscaffold_74G00442920 Rroxscaffold_75G00447260 Rroxscaffold_75G00447290 Rroxscaffold_75G00447430 Rroxscaffold_76G00448540 Rroxscaffold_76G00448610 Rroxscaffold_77G00449150 Rroxscaffold_78G00449610 Rroxscaffold_79G00450210 Rroxscaffold_80G00450330 Rroxscaffold_80G00450350 Rroxscaffold_81G00450480 Rroxscaffold_83G00450980 Rroxscaffold_85G00451230 Rroxscaffold_86G00451270 Rroxscaffold_89G00451410 Rroxscaffold_90G00448760 Rroxscaffold_90G00448770 Rroxscaffold_90G00448820 Rroxscaffold_91G00443000 Rroxscaffold_91G00443060 Rroxscaffold_92G00446430 Rroxscaffold_93G00440830 Rroxscaffold_93G00440850 Rroxscaffold_93G00440860 Rroxscaffold_93G00440880 Rroxscaffold_93G00440910 Rroxscaffold_94G00445830 Rroxscaffold_94G00445910 Rroxscaffold_94G00445940 Rroxscaffold_94G00446020 Rroxscaffold_97G00442340 Rroxscaffold_97G00442390 Rroxscaffold_97G00442400 Rroxscaffold_98G00451350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 102
AccBSI CCGCTC 1 cut(s) 152
AccII CGCG 4 cut(s) 62, 78, 193, 249
AccIII TCCGGA 1 cut(s) 36
AciI CCGC 7 cut(s) 52, 85, 100, 150, 155, 188, 191
AclWI GGATC 1 cut(s) 142
AcoI YGGCCR 2 cut(s) 147, 231
AfaI GTAC 1 cut(s) 7
AfiI CCNNNNNNNGG 5 cut(s) 102, 115, 131, 233, 240
AgsI TTSAA 1 cut(s) 46
AjiI CACGTC 1 cut(s) 82
AjnI CCWGG 3 cut(s) 130, 171, 213
Alw21I GWGCWC 1 cut(s) 92
AlwI GGATC 1 cut(s) 142
AlwNI CAGNNNCTG 2 cut(s) 102, 137
Ama87I CYCGRG 1 cut(s) 158
Aor13HI TCCGGA 1 cut(s) 36
AoxI GGCC 4 cut(s) 49, 147, 211, 231
ApeKI GCWGC 1 cut(s) 181
AspLEI GCGC 4 cut(s) 62, 64, 195, 251
AspS9I GGNCC 4 cut(s) 31, 49, 105, 211
AsuC2I CCSGG 5 cut(s) 13, 56, 159, 160, 235
AvaI CYCGRG 1 cut(s) 158
AvaII GGWCC 2 cut(s) 31, 105
BanII GRGCYC 1 cut(s) 165
Bbv12I GWGCWC 1 cut(s) 92
BbvI GCAGC 1 cut(s) 193
BceAI ACGGC 3 cut(s) 109, 162, 246
BciT130I CCWGG 3 cut(s) 132, 173, 215
BcnI CCSGG 5 cut(s) 13, 56, 159, 160, 235
BglI GCCNNNNNGGC 2 cut(s) 57, 230
BisI GCNGC 5 cut(s) 52, 100, 150, 182, 191
BlpI GCTNAGC 1 cut(s) 120
BlsI GCNGC 5 cut(s) 53, 101, 151, 183, 192
Bme1390I CCNGG 8 cut(s) 13, 56, 132, 159, 160, 173, 215, 235
Bme18I GGWCC 2 cut(s) 31, 105
BmeT110I CYCGRG 1 cut(s) 158
BmgBI CACGTC 1 cut(s) 82
BmgT120I GGNCC 4 cut(s) 31, 49, 105, 211
BmiI GGNNCC 2 cut(s) 107, 212
BmrFI CCNGG 8 cut(s) 13, 56, 132, 159, 160, 173, 215, 235
Bpu1102I GCTNAGC 1 cut(s) 120
BpuMI CCSGG 5 cut(s) 13, 56, 159, 160, 235
BsaJI CCNNGG 3 cut(s) 55, 158, 171
BsaWI WCCGGW 2 cut(s) 36, 66
BsaXI ACNNNNNCTCC 4 cut(s) 97, 127, 136, 166
Bsc4I CCNNNNNNNGG 5 cut(s) 102, 115, 131, 233, 240
BseAI TCCGGA 1 cut(s) 36
BseBI CCWGG 3 cut(s) 132, 173, 215
BseDI CCNNGG 3 cut(s) 55, 158, 171
BseLI CCNNNNNNNGG 5 cut(s) 102, 115, 131, 233, 240
BseMII CTCAG 1 cut(s) 111
BsePI GCGCGC 1 cut(s) 60
BseX3I CGGCCG 2 cut(s) 147, 231
BseXI GCAGC 1 cut(s) 193
BsgI GTGCAG 1 cut(s) 123
Bsh1236I CGCG 4 cut(s) 62, 78, 193, 249
Bsh1285I CGRYCG 4 cut(s) 12, 150, 188, 234
BshFI GGCC 4 cut(s) 51, 149, 213, 233
BsiEI CGRYCG 4 cut(s) 12, 150, 188, 234
BsiHKAI GWGCWC 1 cut(s) 92
BsiHKCI CYCGRG 1 cut(s) 158
BsiSI CCGG 7 cut(s) 12, 37, 55, 67, 116, 159, 234
BslI CCNNNNNNNGG 5 cut(s) 102, 115, 131, 233, 240
BsnI GGCC 4 cut(s) 51, 149, 213, 233
BsoBI CYCGRG 1 cut(s) 158
Bsp1286I GDGCHC 2 cut(s) 92, 165
Bsp13I TCCGGA 1 cut(s) 36
Bsp143I GATC 1 cut(s) 134
Bsp1720I GCTNAGC 1 cut(s) 120
BspACI CCGC 7 cut(s) 52, 85, 100, 150, 155, 188, 191
BspANI GGCC 4 cut(s) 51, 149, 213, 233
BspCNI CTCAG 1 cut(s) 112
BspEI TCCGGA 1 cut(s) 36
BspFNI CGCG 4 cut(s) 62, 78, 193, 249
BspLI GGNNCC 2 cut(s) 107, 212
BspPI GGATC 1 cut(s) 142
BspQI GCTCTTC 1 cut(s) 97
BsrBI CCGCTC 1 cut(s) 152
BssECI CCNNGG 3 cut(s) 55, 158, 171
BssHII GCGCGC 1 cut(s) 60
BssMI GATC 1 cut(s) 134
Bst2UI CCWGG 3 cut(s) 132, 173, 215
Bst6I CTCTTC 1 cut(s) 97
BstC8I GCNNGC 1 cut(s) 62
BstDEI CTNAG 1 cut(s) 120
BstFNI CGCG 4 cut(s) 62, 78, 193, 249
BstHHI GCGC 4 cut(s) 62, 64, 195, 251
BstKTI GATC 1 cut(s) 137
BstMBI GATC 1 cut(s) 134
BstMCI CGRYCG 4 cut(s) 12, 150, 188, 234
BstMWI GCNNNNNNNGC 7 cut(s) 22, 57, 96, 146, 187, 190, 230
BstNI CCWGG 3 cut(s) 132, 173, 215
BstSCI CCNGG 8 cut(s) 11, 54, 130, 157, 158, 171, 213, 233
BstUI CGCG 4 cut(s) 62, 78, 193, 249
BstV1I GCAGC 1 cut(s) 193
BstX2I RGATCY 1 cut(s) 134
BstYI RGATCY 1 cut(s) 134
BstZI CGGCCG 2 cut(s) 147, 231
BsuRI GGCC 4 cut(s) 51, 149, 213, 233
BtrI CACGTC 1 cut(s) 82
Cac8I GCNNGC 1 cut(s) 62
CaiI CAGNNNCTG 2 cut(s) 102, 137
CfoI GCGC 4 cut(s) 62, 64, 195, 251
Cfr13I GGNCC 4 cut(s) 31, 49, 105, 211
Cfr9I CCCGGG 1 cut(s) 158
Csp6I GTAC 1 cut(s) 6
CviJI RGCY 8 cut(s) 51, 99, 119, 124, 149, 163, 213, 233
CviKI_1 RGCY 8 cut(s) 51, 99, 119, 124, 149, 163, 213, 233
CviQI GTAC 1 cut(s) 6
DdeI CTNAG 1 cut(s) 120
DpnI GATC 1 cut(s) 136
DpnII GATC 1 cut(s) 134
EaeI YGGCCR 2 cut(s) 147, 231
EagI CGGCCG 2 cut(s) 147, 231
Eam1104I CTCTTC 1 cut(s) 97
EarI CTCTTC 1 cut(s) 97
EciI GGCGGA 1 cut(s) 144
EclXI CGGCCG 2 cut(s) 147, 231
Eco24I GRGCYC 1 cut(s) 165
Eco47I GGWCC 2 cut(s) 31, 105
Eco52I CGGCCG 2 cut(s) 147, 231
Eco88I CYCGRG 1 cut(s) 158
EcoO109I RGGNCCY 1 cut(s) 211
EcoRII CCWGG 3 cut(s) 130, 171, 213
EcoT38I GRGCYC 1 cut(s) 165
FaiI YATR 1 cut(s) 240
Fnu4HI GCNGC 5 cut(s) 52, 100, 150, 182, 191
FriOI GRGCYC 1 cut(s) 165
Fsp4HI GCNGC 5 cut(s) 52, 100, 150, 182, 191
GlaI GCGC 4 cut(s) 61, 63, 194, 250
GluI GCNGC 5 cut(s) 52, 100, 150, 182, 191
HaeIII GGCC 4 cut(s) 51, 149, 213, 233
HapII CCGG 7 cut(s) 12, 37, 55, 67, 116, 159, 234
HhaI GCGC 4 cut(s) 62, 64, 195, 251
Hin6I GCGC 4 cut(s) 60, 62, 193, 249
HinP1I GCGC 4 cut(s) 60, 62, 193, 249
HpaII CCGG 7 cut(s) 12, 37, 55, 67, 116, 159, 234
Hpy188III TCNNGA 1 cut(s) 37
Hpy99I CGWCG 3 cut(s) 83, 148, 232
HpyCH4IV ACGT 1 cut(s) 81
HpyCH4V TGCA 2 cut(s) 140, 181
HpyF10VI GCNNNNNNNGC 7 cut(s) 22, 57, 96, 146, 187, 190, 230
HpyF3I CTNAG 1 cut(s) 120
HpySE526I ACGT 1 cut(s) 81
HspAI GCGC 4 cut(s) 60, 62, 193, 249
Kpn2I TCCGGA 1 cut(s) 36
Kzo9I GATC 1 cut(s) 134
LguI GCTCTTC 1 cut(s) 97
LmnI GCTCC 1 cut(s) 157
Lsp1109I GCAGC 1 cut(s) 193
MaeII ACGT 1 cut(s) 81
MaeIII GTNAC 1 cut(s) 243
MalI GATC 1 cut(s) 136
MbiI CCGCTC 1 cut(s) 152
MboI GATC 1 cut(s) 134
MboII GAAGA 1 cut(s) 84
MflI RGATCY 1 cut(s) 134
MhlI GDGCHC 2 cut(s) 92, 165
MnlI CCTC 3 cut(s) 44, 123, 207
MroI TCCGGA 1 cut(s) 36
MseI TTAA 1 cut(s) 253
MspA1I CMGCKG 1 cut(s) 102
MspI CCGG 7 cut(s) 12, 37, 55, 67, 116, 159, 234
MspR9I CCNGG 8 cut(s) 13, 56, 132, 159, 160, 173, 215, 235
MvaI CCWGG 3 cut(s) 132, 173, 215
MvnI CGCG 4 cut(s) 62, 78, 193, 249
MwoI GCNNNNNNNGC 7 cut(s) 22, 57, 96, 146, 187, 190, 230
NciI CCSGG 5 cut(s) 13, 56, 159, 160, 235
NdeII GATC 1 cut(s) 134
NlaIV GGNNCC 2 cut(s) 107, 212
NmuCI GTSAC 1 cut(s) 243
PauI GCGCGC 1 cut(s) 60
PciSI GCTCTTC 1 cut(s) 97
PflMI CCANNNNNTGG 1 cut(s) 102
PfoI TCCNGGA 1 cut(s) 130
PkrI GCNGC 5 cut(s) 53, 101, 151, 183, 192
Psp6I CCWGG 3 cut(s) 130, 171, 213
PspGI CCWGG 3 cut(s) 130, 171, 213
PspN4I GGNNCC 2 cut(s) 107, 212
PspPI GGNCC 4 cut(s) 31, 49, 105, 211
PstNI CAGNNNCTG 2 cut(s) 102, 137
PsuI RGATCY 1 cut(s) 134
PteI GCGCGC 1 cut(s) 60
RsaI GTAC 1 cut(s) 7
RsaNI GTAC 1 cut(s) 6
SapI GCTCTTC 1 cut(s) 97
SaqAI TTAA 1 cut(s) 253
SatI GCNGC 5 cut(s) 52, 100, 150, 182, 191
Sau3AI GATC 1 cut(s) 134
Sau96I GGNCC 4 cut(s) 31, 49, 105, 211
ScrFI CCNGG 8 cut(s) 13, 56, 132, 159, 160, 173, 215, 235
SduI GDGCHC 2 cut(s) 92, 165
SetI ASST 4 cut(s) 84, 115, 177, 245
SinI GGWCC 2 cut(s) 31, 105
SmaI CCCGGG 1 cut(s) 160
SrfI GCCCGGGC 1 cut(s) 160
SsiI CCGC 7 cut(s) 52, 85, 100, 150, 155, 188, 191
StyD4I CCNGG 8 cut(s) 11, 54, 130, 157, 158, 171, 213, 233
TaiI ACGT 1 cut(s) 84
TaqII GACCGA 1 cut(s) 19
TauI GCSGC 4 cut(s) 54, 102, 152, 193
Tru1I TTAA 1 cut(s) 253
Tru9I TTAA 1 cut(s) 253
TseFI GTSAC 1 cut(s) 243
TseI GCWGC 1 cut(s) 181
Tsp45I GTSAC 1 cut(s) 243
TspMI CCCGGG 1 cut(s) 158
Van91I CCANNNNNTGG 1 cut(s) 102
VpaK11BI GGWCC 2 cut(s) 31, 105
XmaI CCCGGG 1 cut(s) 158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.