DNA binding
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 599 |
| Acc36I | ACCTGC | 1 cut(s) | 599 |
| AccBSI | CCGCTC | 1 cut(s) | 474 |
| AccI | GTMKAC | 1 cut(s) | 256 |
| AccII | CGCG | 4 cut(s) | 294, 399, 513, 569 |
| AccIII | TCCGGA | 1 cut(s) | 359 |
| AclI | AACGTT | 1 cut(s) | 95 |
| AclWI | GGATC | 1 cut(s) | 23 |
| AcoI | YGGCCR | 4 cut(s) | 234, 438, 469, 552 |
| AfaI | GTAC | 1 cut(s) | 331 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 51, 77, 423, 436, 554, 590 |
| AflII | CTTAAG | 1 cut(s) | 572 |
| AgsI | TTSAA | 1 cut(s) | 369 |
| AjiI | CACGTC | 1 cut(s) | 403 |
| AluBI | AGCT | 1 cut(s) | 186 |
| AluI | AGCT | 1 cut(s) | 186 |
| Alw21I | GWGCWC | 1 cut(s) | 413 |
| Alw26I | GTCTC | 1 cut(s) | 304 |
| AlwI | GGATC | 1 cut(s) | 23 |
| Aor13HI | TCCGGA | 1 cut(s) | 359 |
| AoxI | GGCC | 6 cut(s) | 234, 372, 438, 469, 531, 552 |
| ApeKI | GCWGC | 1 cut(s) | 501 |
| AspLEI | GCGC | 5 cut(s) | 294, 385, 515, 571, 579 |
| AspS9I | GGNCC | 5 cut(s) | 42, 354, 372, 426, 531 |
| AsuC2I | CCSGG | 2 cut(s) | 13, 379 |
| AsuHPI | GGTGA | 2 cut(s) | 294, 623 |
| AvaII | GGWCC | 3 cut(s) | 42, 354, 426 |
| BaeGI | GKGCMC | 1 cut(s) | 64 |
| BbsI | GAAGAC | 1 cut(s) | 188 |
| Bbv12I | GWGCWC | 1 cut(s) | 413 |
| BbvI | GCAGC | 1 cut(s) | 513 |
| BccI | CCATC | 1 cut(s) | 588 |
| BceAI | ACGGC | 4 cut(s) | 221, 430, 484, 567 |
| BcnI | CCSGG | 2 cut(s) | 13, 379 |
| BcoDI | GTCTC | 1 cut(s) | 304 |
| BfaI | CTAG | 2 cut(s) | 176, 596 |
| BfoI | RGCGCY | 1 cut(s) | 580 |
| BfrI | CTTAAG | 1 cut(s) | 572 |
| BfuAI | ACCTGC | 1 cut(s) | 599 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 380, 551 |
| BisI | GCNGC | 5 cut(s) | 375, 421, 472, 502, 511 |
| BlsI | GCNGC | 5 cut(s) | 376, 422, 473, 503, 512 |
| Bme1390I | CCNGG | 2 cut(s) | 13, 379 |
| Bme18I | GGWCC | 3 cut(s) | 42, 354, 426 |
| BmgBI | CACGTC | 1 cut(s) | 403 |
| BmgT120I | GGNCC | 5 cut(s) | 42, 354, 372, 426, 531 |
| BmiI | GGNNCC | 4 cut(s) | 43, 132, 428, 532 |
| BmrFI | CCNGG | 2 cut(s) | 13, 379 |
| BmsI | GCATC | 1 cut(s) | 307 |
| BoxI | GACNNNNGTC | 1 cut(s) | 178 |
| BpiI | GAAGAC | 1 cut(s) | 188 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 170, 202 |
| BpuEI | CTTGAG | 1 cut(s) | 137 |
| BpuMI | CCSGG | 2 cut(s) | 13, 379 |
| BsaI | GGTCTC | 1 cut(s) | 304 |
| BsaJI | CCNNGG | 5 cut(s) | 231, 237, 305, 378, 534 |
| BsaWI | WCCGGW | 2 cut(s) | 359, 387 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 418, 448, 458, 488 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 51, 77, 423, 436, 554, 590 |
| Bse118I | RCCGGY | 3 cut(s) | 335, 479, 554 |
| Bse1I | ACTGG | 1 cut(s) | 452 |
| BseAI | TCCGGA | 1 cut(s) | 359 |
| BseDI | CCNNGG | 5 cut(s) | 231, 237, 305, 378, 534 |
| BseGI | GGATG | 1 cut(s) | 580 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 51, 77, 423, 436, 554, 590 |
| BseNI | ACTGG | 1 cut(s) | 452 |
| BseSI | GKGCMC | 1 cut(s) | 64 |
| BseX3I | CGGCCG | 3 cut(s) | 438, 469, 552 |
| BseXI | GCAGC | 1 cut(s) | 513 |
| BsgI | GTGCAG | 1 cut(s) | 445 |
| Bsh1236I | CGCG | 4 cut(s) | 294, 399, 513, 569 |
| Bsh1285I | CGRYCG | 5 cut(s) | 336, 441, 472, 508, 555 |
| BshFI | GGCC | 6 cut(s) | 236, 374, 440, 471, 533, 554 |
| BsiEI | CGRYCG | 5 cut(s) | 336, 441, 472, 508, 555 |
| BsiHKAI | GWGCWC | 1 cut(s) | 413 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 51, 77, 423, 436, 554, 590 |
| BsmAI | GTCTC | 1 cut(s) | 304 |
| BsnI | GGCC | 6 cut(s) | 236, 374, 440, 471, 533, 554 |
| Bso31I | GGTCTC | 1 cut(s) | 304 |
| Bsp1286I | GDGCHC | 2 cut(s) | 64, 413 |
| Bsp13I | TCCGGA | 1 cut(s) | 359 |
| Bsp143I | GATC | 2 cut(s) | 15, 456 |
| BspANI | GGCC | 6 cut(s) | 236, 374, 440, 471, 533, 554 |
| BspEI | TCCGGA | 1 cut(s) | 359 |
| BspFNI | CGCG | 4 cut(s) | 294, 399, 513, 569 |
| BspLI | GGNNCC | 4 cut(s) | 43, 132, 428, 532 |
| BspMI | ACCTGC | 1 cut(s) | 599 |
| BspPI | GGATC | 1 cut(s) | 23 |
| BspQI | GCTCTTC | 1 cut(s) | 418 |
| BspTI | CTTAAG | 1 cut(s) | 572 |
| BspTNI | GGTCTC | 1 cut(s) | 304 |
| BsrBI | CCGCTC | 1 cut(s) | 474 |
| BsrFI | RCCGGY | 3 cut(s) | 335, 479, 554 |
| BsrI | ACTGG | 1 cut(s) | 452 |
| BssAI | RCCGGY | 3 cut(s) | 335, 479, 554 |
| BssECI | CCNNGG | 5 cut(s) | 231, 237, 305, 378, 534 |
| BssMI | GATC | 2 cut(s) | 15, 456 |
| BssT1I | CCWWGG | 2 cut(s) | 231, 305 |
| Bst4CI | ACNGT | 1 cut(s) | 266 |
| Bst6I | CTCTTC | 1 cut(s) | 418 |
| BstAFI | CTTAAG | 1 cut(s) | 572 |
| BstC8I | GCNNGC | 2 cut(s) | 23, 481 |
| BstDEI | CTNAG | 1 cut(s) | 630 |
| BstDSI | CCRYGG | 1 cut(s) | 237 |
| BstF5I | GGATG | 1 cut(s) | 580 |
| BstFNI | CGCG | 4 cut(s) | 294, 399, 513, 569 |
| BstH2I | RGCGCY | 1 cut(s) | 580 |
| BstHHI | GCGC | 5 cut(s) | 294, 385, 515, 571, 579 |
| BstKTI | GATC | 2 cut(s) | 18, 459 |
| BstMAI | GTCTC | 1 cut(s) | 304 |
| BstMBI | GATC | 2 cut(s) | 15, 456 |
| BstMCI | CGRYCG | 5 cut(s) | 336, 441, 472, 508, 555 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 345, 380, 417, 468, 480, 507, 510, 551 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 178 |
| BstSCI | CCNGG | 2 cut(s) | 11, 377 |
| BstSLI | GKGCMC | 1 cut(s) | 64 |
| BstUI | CGCG | 4 cut(s) | 294, 399, 513, 569 |
| BstV1I | GCAGC | 1 cut(s) | 513 |
| BstV2I | GAAGAC | 1 cut(s) | 188 |
| BstZI | CGGCCG | 3 cut(s) | 438, 469, 552 |
| BsuRI | GGCC | 6 cut(s) | 236, 374, 440, 471, 533, 554 |
| BtgI | CCRYGG | 1 cut(s) | 237 |
| BtrI | CACGTC | 1 cut(s) | 403 |
| BtsCI | GGATG | 1 cut(s) | 580 |
| BtsI | GCAGTG | 2 cut(s) | 38, 64 |
| BtsIMutI | CAGTG | 4 cut(s) | 38, 64, 271, 459 |
| BveI | ACCTGC | 1 cut(s) | 599 |
| Cac8I | GCNNGC | 2 cut(s) | 23, 481 |
| CfoI | GCGC | 5 cut(s) | 294, 385, 515, 571, 579 |
| Cfr10I | RCCGGY | 3 cut(s) | 335, 479, 554 |
| Cfr13I | GGNCC | 5 cut(s) | 42, 354, 372, 426, 531 |
| CseI | GACGC | 1 cut(s) | 283 |
| Csp6I | GTAC | 1 cut(s) | 330 |
| CviAII | CATG | 1 cut(s) | 289 |
| CviQI | GTAC | 1 cut(s) | 330 |
| DdeI | CTNAG | 1 cut(s) | 630 |
| DpnI | GATC | 2 cut(s) | 17, 458 |
| DpnII | GATC | 2 cut(s) | 15, 456 |
| EaeI | YGGCCR | 4 cut(s) | 234, 438, 469, 552 |
| EagI | CGGCCG | 3 cut(s) | 438, 469, 552 |
| Eam1104I | CTCTTC | 1 cut(s) | 418 |
| EarI | CTCTTC | 1 cut(s) | 418 |
| EciI | GGCGGA | 1 cut(s) | 466 |
| EclXI | CGGCCG | 3 cut(s) | 438, 469, 552 |
| Eco130I | CCWWGG | 2 cut(s) | 231, 305 |
| Eco31I | GGTCTC | 1 cut(s) | 304 |
| Eco47I | GGWCC | 3 cut(s) | 42, 354, 426 |
| Eco52I | CGGCCG | 3 cut(s) | 438, 469, 552 |
| EcoO109I | RGGNCCY | 1 cut(s) | 531 |
| EcoT14I | CCWWGG | 2 cut(s) | 231, 305 |
| ErhI | CCWWGG | 2 cut(s) | 231, 305 |
| FaeI | CATG | 1 cut(s) | 292 |
| FaiI | YATR | 4 cut(s) | 141, 290, 326, 560 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 20, 52 |
| FatI | CATG | 1 cut(s) | 288 |
| FauI | CCCGC | 3 cut(s) | 30, 72, 213 |
| FblI | GTMKAC | 1 cut(s) | 256 |
| Fnu4HI | GCNGC | 5 cut(s) | 375, 421, 472, 502, 511 |
| FokI | GGATG | 1 cut(s) | 567 |
| Fsp4HI | GCNGC | 5 cut(s) | 375, 421, 472, 502, 511 |
| FspBI | CTAG | 2 cut(s) | 176, 596 |
| GlaI | GCGC | 5 cut(s) | 293, 384, 514, 570, 578 |
| GluI | GCNGC | 5 cut(s) | 375, 421, 472, 502, 511 |
| HaeII | RGCGCY | 1 cut(s) | 580 |
| HaeIII | GGCC | 6 cut(s) | 236, 374, 440, 471, 533, 554 |
| HgaI | GACGC | 1 cut(s) | 283 |
| HhaI | GCGC | 5 cut(s) | 294, 385, 515, 571, 579 |
| Hin1II | CATG | 1 cut(s) | 292 |
| Hin6I | GCGC | 5 cut(s) | 292, 383, 513, 569, 577 |
| HinP1I | GCGC | 5 cut(s) | 292, 383, 513, 569, 577 |
| HinfI | GANTC | 5 cut(s) | 73, 179, 211, 271, 602 |
| HphI | GGTGA | 2 cut(s) | 294, 623 |
| Hpy166II | GTNNAC | 2 cut(s) | 257, 302 |
| Hpy188I | TCNGA | 2 cut(s) | 72, 173 |
| Hpy188III | TCNNGA | 1 cut(s) | 360 |
| Hpy8I | GTNNAC | 2 cut(s) | 257, 302 |
| Hpy99I | CGWCG | 3 cut(s) | 404, 470, 553 |
| HpyAV | CCTTC | 1 cut(s) | 121 |
| HpyCH4III | ACNGT | 1 cut(s) | 266 |
| HpyCH4IV | ACGT | 2 cut(s) | 95, 402 |
| HpyCH4V | TGCA | 2 cut(s) | 462, 501 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 345, 380, 417, 468, 480, 507, 510, 551 |
| HpyF3I | CTNAG | 1 cut(s) | 630 |
| HpySE526I | ACGT | 2 cut(s) | 95, 402 |
| Hsp92II | CATG | 1 cut(s) | 292 |
| HspAI | GCGC | 5 cut(s) | 292, 383, 513, 569, 577 |
| Kpn2I | TCCGGA | 1 cut(s) | 359 |
| KroI | GCCGGC | 1 cut(s) | 479 |
| KroNI | GCCGGC | 1 cut(s) | 481 |
| Kzo9I | GATC | 2 cut(s) | 15, 456 |
| LguI | GCTCTTC | 1 cut(s) | 418 |
| LmnI | GCTCC | 2 cut(s) | 136, 479 |
| Lsp1109I | GCAGC | 1 cut(s) | 513 |
| LweI | GCATC | 1 cut(s) | 307 |
| MaeI | CTAG | 2 cut(s) | 176, 596 |
| MaeII | ACGT | 2 cut(s) | 95, 402 |
| MaeIII | GTNAC | 2 cut(s) | 563, 618 |
| MalI | GATC | 2 cut(s) | 17, 458 |
| MbiI | CCGCTC | 1 cut(s) | 474 |
| MboI | GATC | 2 cut(s) | 15, 456 |
| MboII | GAAGA | 2 cut(s) | 188, 405 |
| MhlI | GDGCHC | 2 cut(s) | 64, 413 |
| MlyI | GAGTC | 1 cut(s) | 188 |
| MmeI | TCCRAC | 1 cut(s) | 151 |
| MnlI | CCTC | 7 cut(s) | 45, 78, 159, 367, 444, 527, 544 |
| MroI | TCCGGA | 1 cut(s) | 359 |
| MroNI | GCCGGC | 1 cut(s) | 479 |
| MseI | TTAA | 3 cut(s) | 98, 279, 573 |
| MspCI | CTTAAG | 1 cut(s) | 572 |
| MspR9I | CCNGG | 2 cut(s) | 13, 379 |
| MvnI | CGCG | 4 cut(s) | 294, 399, 513, 569 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 345, 380, 417, 468, 480, 507, 510, 551 |
| NaeI | GCCGGC | 1 cut(s) | 481 |
| NciI | CCSGG | 2 cut(s) | 13, 379 |
| NdeII | GATC | 2 cut(s) | 15, 456 |
| NgoMIV | GCCGGC | 1 cut(s) | 479 |
| NlaIII | CATG | 1 cut(s) | 292 |
| NlaIV | GGNNCC | 4 cut(s) | 43, 132, 428, 532 |
| NmeAIII | GCCGAG | 2 cut(s) | 466, 515 |
| NmuCI | GTSAC | 1 cut(s) | 563 |
| PaqCI | CACCTGC | 1 cut(s) | 599 |
| PciSI | GCTCTTC | 1 cut(s) | 418 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 222 |
| PdiI | GCCGGC | 1 cut(s) | 481 |
| PfeI | GAWTC | 4 cut(s) | 73, 211, 271, 602 |
| PkrI | GCNGC | 5 cut(s) | 376, 422, 473, 503, 512 |
| PleI | GAGTC | 1 cut(s) | 187 |
| PpsI | GAGTC | 1 cut(s) | 187 |
| PshAI | GACNNNNGTC | 1 cut(s) | 178 |
| Psp1406I | AACGTT | 1 cut(s) | 95 |
| PspN4I | GGNNCC | 4 cut(s) | 43, 132, 428, 532 |
| PspPI | GGNCC | 5 cut(s) | 42, 354, 372, 426, 531 |
| RsaI | GTAC | 1 cut(s) | 331 |
| RsaNI | GTAC | 1 cut(s) | 330 |
| SapI | GCTCTTC | 1 cut(s) | 418 |
| SaqAI | TTAA | 3 cut(s) | 98, 279, 573 |
| SatI | GCNGC | 5 cut(s) | 375, 421, 472, 502, 511 |
| Sau3AI | GATC | 2 cut(s) | 15, 456 |
| Sau96I | GGNCC | 5 cut(s) | 42, 354, 372, 426, 531 |
| SchI | GAGTC | 1 cut(s) | 188 |
| ScrFI | CCNGG | 2 cut(s) | 13, 379 |
| SduI | GDGCHC | 2 cut(s) | 64, 413 |
| SfaNI | GCATC | 1 cut(s) | 307 |
| SinI | GGWCC | 3 cut(s) | 42, 354, 426 |
| SmlI | CTYRAG | 2 cut(s) | 152, 572 |
| SmoI | CTYRAG | 2 cut(s) | 152, 572 |
| SspMI | CTAG | 2 cut(s) | 176, 596 |
| StyD4I | CCNGG | 2 cut(s) | 11, 377 |
| StyI | CCWWGG | 2 cut(s) | 231, 305 |
| TaaI | ACNGT | 1 cut(s) | 266 |
| TaiI | ACGT | 2 cut(s) | 98, 405 |
| TaqII | GACCGA | 1 cut(s) | 342 |
| TauI | GCSGC | 4 cut(s) | 377, 423, 474, 513 |
| TfiI | GAWTC | 4 cut(s) | 73, 211, 271, 602 |
| Tru1I | TTAA | 3 cut(s) | 98, 279, 573 |
| Tru9I | TTAA | 3 cut(s) | 98, 279, 573 |
| TscAI | CASTG | 4 cut(s) | 38, 64, 271, 459 |
| TseFI | GTSAC | 1 cut(s) | 563 |
| TseI | GCWGC | 1 cut(s) | 501 |
| Tsp45I | GTSAC | 1 cut(s) | 563 |
| TspDTI | ATGAA | 1 cut(s) | 277 |
| TspGWI | ACGGA | 1 cut(s) | 93 |
| TspRI | CASTG | 4 cut(s) | 38, 64, 271, 459 |
| Vha464I | CTTAAG | 1 cut(s) | 572 |
| VpaK11BI | GGWCC | 3 cut(s) | 42, 354, 426 |
| XmiI | GTMKAC | 1 cut(s) | 256 |
| XspI | CTAG | 2 cut(s) | 176, 596 |