DNA binding
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 470 |
| AccB1I | GGYRCC | 1 cut(s) | 57 |
| AccI | GTMKAC | 1 cut(s) | 250 |
| AccII | CGCG | 5 cut(s) | 206, 363, 547, 549, 556 |
| AccIII | TCCGGA | 2 cut(s) | 324, 472 |
| AclI | AACGTT | 2 cut(s) | 92, 485 |
| AclWI | GGATC | 2 cut(s) | 22, 595 |
| AcoI | YGGCCR | 3 cut(s) | 18, 382, 402 |
| AcsI | RAATTY | 1 cut(s) | 517 |
| AcyI | GRCGYC | 1 cut(s) | 467 |
| AfaI | GTAC | 1 cut(s) | 295 |
| AfiI | CCNNNNNNNGG | 9 cut(s) | 50, 74, 232, 387, 400, 416, 507, 513, 637 |
| AgsI | TTSAA | 2 cut(s) | 334, 646 |
| AjiI | CACGTC | 2 cut(s) | 367, 504 |
| AluBI | AGCT | 1 cut(s) | 183 |
| AluI | AGCT | 1 cut(s) | 183 |
| Alw21I | GWGCWC | 1 cut(s) | 377 |
| Alw26I | GTCTC | 4 cut(s) | 269, 474, 545, 584 |
| AlwI | GGATC | 2 cut(s) | 22, 595 |
| Ama87I | CYCGRG | 1 cut(s) | 452 |
| Aor13HI | TCCGGA | 2 cut(s) | 324, 472 |
| AoxI | GGCC | 6 cut(s) | 18, 337, 382, 402, 456, 639 |
| ApoI | RAATTY | 1 cut(s) | 517 |
| AspLEI | GCGC | 3 cut(s) | 350, 549, 558 |
| AspS9I | GGNCC | 5 cut(s) | 41, 319, 337, 390, 457 |
| AsuC2I | CCSGG | 5 cut(s) | 301, 344, 417, 508, 576 |
| AsuHPI | GGTGA | 1 cut(s) | 259 |
| AsuII | TTCGAA | 1 cut(s) | 538 |
| AvaI | CYCGRG | 1 cut(s) | 452 |
| AvaII | GGWCC | 3 cut(s) | 41, 319, 390 |
| BaeGI | GKGCMC | 1 cut(s) | 62 |
| BanI | GGYRCC | 1 cut(s) | 57 |
| BbsI | GAAGAC | 1 cut(s) | 186 |
| Bbv12I | GWGCWC | 1 cut(s) | 377 |
| BceAI | ACGGC | 4 cut(s) | 275, 394, 475, 596 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 14, 48 |
| BcnI | CCSGG | 5 cut(s) | 301, 344, 417, 508, 576 |
| BcoDI | GTCTC | 4 cut(s) | 269, 474, 545, 584 |
| BfaI | CTAG | 1 cut(s) | 173 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 17, 345 |
| BisI | GCNGC | 3 cut(s) | 21, 340, 385 |
| BlsI | GCNGC | 3 cut(s) | 22, 341, 386 |
| Bme1390I | CCNGG | 5 cut(s) | 301, 344, 417, 508, 576 |
| Bme18I | GGWCC | 3 cut(s) | 41, 319, 390 |
| BmeT110I | CYCGRG | 1 cut(s) | 452 |
| BmgBI | CACGTC | 2 cut(s) | 367, 504 |
| BmgT120I | GGNCC | 5 cut(s) | 41, 319, 337, 390, 457 |
| BmiI | GGNNCC | 6 cut(s) | 42, 59, 129, 392, 459, 624 |
| BmrFI | CCNGG | 5 cut(s) | 301, 344, 417, 508, 576 |
| BmsI | GCATC | 1 cut(s) | 271 |
| BoxI | GACNNNNGTC | 1 cut(s) | 175 |
| BpiI | GAAGAC | 1 cut(s) | 186 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 167, 199 |
| Bpu14I | TTCGAA | 1 cut(s) | 538 |
| BpuEI | CTTGAG | 1 cut(s) | 134 |
| BpuMI | CCSGG | 5 cut(s) | 301, 344, 417, 508, 576 |
| BsaHI | GRCGYC | 1 cut(s) | 467 |
| BsaI | GGTCTC | 2 cut(s) | 269, 584 |
| BsaJI | CCNNGG | 5 cut(s) | 226, 270, 343, 416, 453 |
| BsaWI | WCCGGW | 2 cut(s) | 324, 472 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 382, 412 |
| Bsc4I | CCNNNNNNNGG | 9 cut(s) | 50, 74, 232, 387, 400, 416, 507, 513, 637 |
| BseAI | TCCGGA | 2 cut(s) | 324, 472 |
| BseDI | CCNNGG | 5 cut(s) | 226, 270, 343, 416, 453 |
| BseLI | CCNNNNNNNGG | 9 cut(s) | 50, 74, 232, 387, 400, 416, 507, 513, 637 |
| BseSI | GKGCMC | 1 cut(s) | 62 |
| BseX3I | CGGCCG | 3 cut(s) | 18, 382, 402 |
| Bsh1236I | CGCG | 5 cut(s) | 206, 363, 547, 549, 556 |
| Bsh1285I | CGRYCG | 4 cut(s) | 21, 300, 385, 405 |
| BshFI | GGCC | 6 cut(s) | 20, 339, 384, 404, 458, 641 |
| BshNI | GGYRCC | 1 cut(s) | 57 |
| BsiEI | CGRYCG | 4 cut(s) | 21, 300, 385, 405 |
| BsiHKAI | GWGCWC | 1 cut(s) | 377 |
| BsiHKCI | CYCGRG | 1 cut(s) | 452 |
| BslFI | GGGAC | 2 cut(s) | 270, 490 |
| BslI | CCNNNNNNNGG | 9 cut(s) | 50, 74, 232, 387, 400, 416, 507, 513, 637 |
| BsmAI | GTCTC | 4 cut(s) | 269, 474, 545, 584 |
| BsmBI | CGTCTC | 2 cut(s) | 474, 545 |
| BsmFI | GGGAC | 2 cut(s) | 270, 490 |
| BsnI | GGCC | 6 cut(s) | 20, 339, 384, 404, 458, 641 |
| Bso31I | GGTCTC | 2 cut(s) | 269, 584 |
| BsoBI | CYCGRG | 1 cut(s) | 452 |
| Bsp119I | TTCGAA | 1 cut(s) | 538 |
| Bsp1286I | GDGCHC | 2 cut(s) | 62, 377 |
| Bsp13I | TCCGGA | 2 cut(s) | 324, 472 |
| Bsp143I | GATC | 2 cut(s) | 14, 587 |
| BspANI | GGCC | 6 cut(s) | 20, 339, 384, 404, 458, 641 |
| BspEI | TCCGGA | 2 cut(s) | 324, 472 |
| BspFNI | CGCG | 5 cut(s) | 206, 363, 547, 549, 556 |
| BspLI | GGNNCC | 6 cut(s) | 42, 59, 129, 392, 459, 624 |
| BspPI | GGATC | 2 cut(s) | 22, 595 |
| BspQI | GCTCTTC | 1 cut(s) | 382 |
| BspT104I | TTCGAA | 1 cut(s) | 538 |
| BspT107I | GGYRCC | 1 cut(s) | 57 |
| BspTNI | GGTCTC | 2 cut(s) | 269, 584 |
| BssECI | CCNNGG | 5 cut(s) | 226, 270, 343, 416, 453 |
| BssMI | GATC | 2 cut(s) | 14, 587 |
| BssNI | GRCGYC | 1 cut(s) | 467 |
| BssT1I | CCWWGG | 2 cut(s) | 226, 270 |
| Bst6I | CTCTTC | 3 cut(s) | 382, 453, 640 |
| BstACI | GRCGYC | 1 cut(s) | 467 |
| BstBI | TTCGAA | 1 cut(s) | 538 |
| BstC8I | GCNNGC | 2 cut(s) | 62, 216 |
| BstDEI | CTNAG | 1 cut(s) | 583 |
| BstEII | GGTNACC | 1 cut(s) | 570 |
| BstFNI | CGCG | 5 cut(s) | 206, 363, 547, 549, 556 |
| BstHHI | GCGC | 3 cut(s) | 350, 549, 558 |
| BstKTI | GATC | 2 cut(s) | 17, 590 |
| BstMAI | GTCTC | 4 cut(s) | 269, 474, 545, 584 |
| BstMBI | GATC | 2 cut(s) | 14, 587 |
| BstMCI | CGRYCG | 4 cut(s) | 21, 300, 385, 405 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 17, 26, 61, 310, 345, 381, 553, 555 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 175 |
| BstPI | GGTNACC | 1 cut(s) | 570 |
| BstSCI | CCNGG | 5 cut(s) | 299, 342, 415, 506, 574 |
| BstSLI | GKGCMC | 1 cut(s) | 62 |
| BstUI | CGCG | 5 cut(s) | 206, 363, 547, 549, 556 |
| BstV2I | GAAGAC | 1 cut(s) | 186 |
| BstZI | CGGCCG | 3 cut(s) | 18, 382, 402 |
| BsuRI | GGCC | 6 cut(s) | 20, 339, 384, 404, 458, 641 |
| BtrI | CACGTC | 2 cut(s) | 367, 504 |
| Cac8I | GCNNGC | 2 cut(s) | 62, 216 |
| CfoI | GCGC | 3 cut(s) | 350, 549, 558 |
| Cfr13I | GGNCC | 5 cut(s) | 41, 319, 337, 390, 457 |
| CseI | GACGC | 1 cut(s) | 562 |
| Csp6I | GTAC | 1 cut(s) | 294 |
| CviAII | CATG | 2 cut(s) | 600, 619 |
| CviQI | GTAC | 1 cut(s) | 294 |
| DdeI | CTNAG | 1 cut(s) | 583 |
| DpnI | GATC | 2 cut(s) | 16, 589 |
| DpnII | GATC | 2 cut(s) | 14, 587 |
| EaeI | YGGCCR | 3 cut(s) | 18, 382, 402 |
| EagI | CGGCCG | 3 cut(s) | 18, 382, 402 |
| Eam1104I | CTCTTC | 3 cut(s) | 382, 453, 640 |
| EarI | CTCTTC | 3 cut(s) | 382, 453, 640 |
| EclXI | CGGCCG | 3 cut(s) | 18, 382, 402 |
| Eco130I | CCWWGG | 2 cut(s) | 226, 270 |
| Eco31I | GGTCTC | 2 cut(s) | 269, 584 |
| Eco47I | GGWCC | 3 cut(s) | 41, 319, 390 |
| Eco52I | CGGCCG | 3 cut(s) | 18, 382, 402 |
| Eco88I | CYCGRG | 1 cut(s) | 452 |
| Eco91I | GGTNACC | 1 cut(s) | 570 |
| EcoO109I | RGGNCCY | 1 cut(s) | 457 |
| EcoO65I | GGTNACC | 1 cut(s) | 570 |
| EcoT14I | CCWWGG | 2 cut(s) | 226, 270 |
| ErhI | CCWWGG | 2 cut(s) | 226, 270 |
| Esp3I | CGTCTC | 2 cut(s) | 474, 545 |
| FaeI | CATG | 2 cut(s) | 603, 622 |
| FaiI | YATR | 4 cut(s) | 138, 290, 601, 620 |
| FaqI | GGGAC | 2 cut(s) | 270, 490 |
| FatI | CATG | 2 cut(s) | 599, 618 |
| FauI | CCCGC | 3 cut(s) | 69, 211, 468 |
| FblI | GTMKAC | 1 cut(s) | 250 |
| Fnu4HI | GCNGC | 3 cut(s) | 21, 340, 385 |
| Fsp4HI | GCNGC | 3 cut(s) | 21, 340, 385 |
| FspBI | CTAG | 1 cut(s) | 173 |
| GlaI | GCGC | 3 cut(s) | 349, 548, 557 |
| GluI | GCNGC | 3 cut(s) | 21, 340, 385 |
| HaeIII | GGCC | 6 cut(s) | 20, 339, 384, 404, 458, 641 |
| HgaI | GACGC | 1 cut(s) | 562 |
| HhaI | GCGC | 3 cut(s) | 350, 549, 558 |
| Hin1I | GRCGYC | 1 cut(s) | 467 |
| Hin1II | CATG | 2 cut(s) | 603, 622 |
| Hin6I | GCGC | 3 cut(s) | 348, 547, 556 |
| HinP1I | GCGC | 3 cut(s) | 348, 547, 556 |
| HincII | GTYRAC | 1 cut(s) | 495 |
| HindII | GTYRAC | 1 cut(s) | 495 |
| HinfI | GANTC | 4 cut(s) | 70, 176, 476, 529 |
| HphI | GGTGA | 1 cut(s) | 259 |
| Hpy166II | GTNNAC | 4 cut(s) | 251, 267, 495, 616 |
| Hpy188I | TCNGA | 3 cut(s) | 69, 170, 564 |
| Hpy188III | TCNNGA | 4 cut(s) | 325, 452, 473, 514 |
| Hpy8I | GTNNAC | 4 cut(s) | 251, 267, 495, 616 |
| Hpy99I | CGWCG | 2 cut(s) | 368, 469 |
| HpyAV | CCTTC | 2 cut(s) | 118, 652 |
| HpyCH4IV | ACGT | 5 cut(s) | 92, 366, 467, 485, 503 |
| HpyCH4V | TGCA | 1 cut(s) | 605 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 17, 26, 61, 310, 345, 381, 553, 555 |
| HpyF3I | CTNAG | 1 cut(s) | 583 |
| HpySE526I | ACGT | 5 cut(s) | 92, 366, 467, 485, 503 |
| Hsp92I | GRCGYC | 1 cut(s) | 467 |
| Hsp92II | CATG | 2 cut(s) | 603, 622 |
| HspAI | GCGC | 3 cut(s) | 348, 547, 556 |
| Kpn2I | TCCGGA | 2 cut(s) | 324, 472 |
| Kzo9I | GATC | 2 cut(s) | 14, 587 |
| LguI | GCTCTTC | 1 cut(s) | 382 |
| LmnI | GCTCC | 1 cut(s) | 133 |
| LweI | GCATC | 1 cut(s) | 271 |
| MaeI | CTAG | 1 cut(s) | 173 |
| MaeII | ACGT | 5 cut(s) | 92, 366, 467, 485, 503 |
| MaeIII | GTNAC | 1 cut(s) | 570 |
| MalI | GATC | 2 cut(s) | 16, 589 |
| MboI | GATC | 2 cut(s) | 14, 587 |
| MboII | GAAGA | 4 cut(s) | 186, 369, 440, 627 |
| MhlI | GDGCHC | 2 cut(s) | 62, 377 |
| MluCI | AATT | 1 cut(s) | 517 |
| MlyI | GAGTC | 2 cut(s) | 185, 470 |
| MmeI | TCCRAC | 1 cut(s) | 148 |
| MnlI | CCTC | 7 cut(s) | 44, 75, 156, 332, 408, 448, 643 |
| MroI | TCCGGA | 2 cut(s) | 324, 472 |
| MseI | TTAA | 3 cut(s) | 95, 431, 522 |
| MspR9I | CCNGG | 5 cut(s) | 301, 344, 417, 508, 576 |
| MvnI | CGCG | 5 cut(s) | 206, 363, 547, 549, 556 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 17, 26, 61, 310, 345, 381, 553, 555 |
| NciI | CCSGG | 5 cut(s) | 301, 344, 417, 508, 576 |
| NdeII | GATC | 2 cut(s) | 14, 587 |
| NlaIII | CATG | 2 cut(s) | 603, 622 |
| NlaIV | GGNNCC | 6 cut(s) | 42, 59, 129, 392, 459, 624 |
| NmeAIII | GCCGAG | 1 cut(s) | 430 |
| NspV | TTCGAA | 1 cut(s) | 538 |
| PciSI | GCTCTTC | 1 cut(s) | 382 |
| PfeI | GAWTC | 2 cut(s) | 70, 529 |
| PkrI | GCNGC | 3 cut(s) | 22, 341, 386 |
| PleI | GAGTC | 2 cut(s) | 184, 470 |
| PpsI | GAGTC | 2 cut(s) | 184, 470 |
| PshAI | GACNNNNGTC | 1 cut(s) | 175 |
| Psp1406I | AACGTT | 2 cut(s) | 92, 485 |
| PspEI | GGTNACC | 1 cut(s) | 570 |
| PspN4I | GGNNCC | 6 cut(s) | 42, 59, 129, 392, 459, 624 |
| PspPI | GGNCC | 5 cut(s) | 41, 319, 337, 390, 457 |
| RsaI | GTAC | 1 cut(s) | 295 |
| RsaNI | GTAC | 1 cut(s) | 294 |
| SapI | GCTCTTC | 1 cut(s) | 382 |
| SaqAI | TTAA | 3 cut(s) | 95, 431, 522 |
| SatI | GCNGC | 3 cut(s) | 21, 340, 385 |
| Sau3AI | GATC | 2 cut(s) | 14, 587 |
| Sau96I | GGNCC | 5 cut(s) | 41, 319, 337, 390, 457 |
| SchI | GAGTC | 2 cut(s) | 185, 470 |
| ScrFI | CCNGG | 5 cut(s) | 301, 344, 417, 508, 576 |
| SduI | GDGCHC | 2 cut(s) | 62, 377 |
| SfaNI | GCATC | 1 cut(s) | 271 |
| SfuI | TTCGAA | 1 cut(s) | 538 |
| SinI | GGWCC | 3 cut(s) | 41, 319, 390 |
| SmlI | CTYRAG | 1 cut(s) | 149 |
| SmoI | CTYRAG | 1 cut(s) | 149 |
| Sse9I | AATT | 1 cut(s) | 517 |
| SspMI | CTAG | 1 cut(s) | 173 |
| StyD4I | CCNGG | 5 cut(s) | 299, 342, 415, 506, 574 |
| StyI | CCWWGG | 2 cut(s) | 226, 270 |
| TaiI | ACGT | 5 cut(s) | 95, 369, 470, 488, 506 |
| TaqI | TCGA | 2 cut(s) | 538, 590 |
| TaqII | GACCGA | 2 cut(s) | 293, 307 |
| TasI | AATT | 1 cut(s) | 517 |
| TauI | GCSGC | 3 cut(s) | 23, 342, 387 |
| TfiI | GAWTC | 2 cut(s) | 70, 529 |
| Tru1I | TTAA | 3 cut(s) | 95, 431, 522 |
| Tru9I | TTAA | 3 cut(s) | 95, 431, 522 |
| TspGWI | ACGGA | 2 cut(s) | 90, 367 |
| VpaK11BI | GGWCC | 3 cut(s) | 41, 319, 390 |
| XapI | RAATTY | 1 cut(s) | 517 |
| XmiI | GTMKAC | 1 cut(s) | 250 |
| XspI | CTAG | 1 cut(s) | 173 |
| ZraI | GACGTC | 1 cut(s) | 468 |