DNA binding
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 3 cut(s) | 53, 644, 789 |
| AccBSI | CCGCTC | 1 cut(s) | 532 |
| AccI | GTMKAC | 1 cut(s) | 250 |
| AccII | CGCG | 8 cut(s) | 325, 522, 541, 680, 682, 737, 778, 780 |
| AclI | AACGTT | 1 cut(s) | 89 |
| AclWI | GGATC | 1 cut(s) | 23 |
| AcoI | YGGCCR | 1 cut(s) | 228 |
| AcsI | RAATTY | 1 cut(s) | 335 |
| AcyI | GRCGYC | 2 cut(s) | 54, 645 |
| AfaI | GTAC | 1 cut(s) | 446 |
| AfiI | CCNNNNNNNGG | 8 cut(s) | 48, 328, 721, 722, 758, 799, 806, 876 |
| AflII | CTTAAG | 2 cut(s) | 290, 740 |
| AgeI | ACCGGT | 1 cut(s) | 447 |
| AgsI | TTSAA | 2 cut(s) | 335, 466 |
| AluBI | AGCT | 3 cut(s) | 180, 550, 860 |
| AluI | AGCT | 3 cut(s) | 180, 550, 860 |
| Alw21I | GWGCWC | 1 cut(s) | 862 |
| AlwI | GGATC | 1 cut(s) | 23 |
| Ama87I | CYCGRG | 5 cut(s) | 551, 586, 608, 626, 660 |
| AoxI | GGCC | 3 cut(s) | 228, 583, 847 |
| ApaI | GGGCCC | 1 cut(s) | 587 |
| ApeKI | GCWGC | 3 cut(s) | 357, 547, 890 |
| ApoI | RAATTY | 1 cut(s) | 335 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 263, 295 |
| AsiGI | ACCGGT | 1 cut(s) | 447 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 339 |
| AspLEI | GCGC | 9 cut(s) | 56, 327, 647, 682, 684, 739, 747, 778, 780 |
| AspS9I | GGNCC | 6 cut(s) | 39, 395, 583, 584, 590, 848 |
| AsuHPI | GGTGA | 1 cut(s) | 553 |
| AvaI | CYCGRG | 5 cut(s) | 551, 586, 608, 626, 660 |
| AvaII | GGWCC | 3 cut(s) | 39, 395, 590 |
| BaeGI | GKGCMC | 4 cut(s) | 587, 602, 704, 792 |
| BanI | GGYRCC | 3 cut(s) | 53, 644, 789 |
| BanII | GRGCYC | 3 cut(s) | 587, 654, 862 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 787, 819 |
| BbsI | GAAGAC | 1 cut(s) | 182 |
| Bbv12I | GWGCWC | 1 cut(s) | 862 |
| BbvI | GCAGC | 3 cut(s) | 369, 559, 902 |
| BccI | CCATC | 2 cut(s) | 406, 756 |
| BceAI | ACGGC | 6 cut(s) | 215, 299, 658, 720, 735, 883 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 381, 415 |
| BfaI | CTAG | 2 cut(s) | 170, 764 |
| BfoI | RGCGCY | 3 cut(s) | 57, 648, 748 |
| BfrI | CTTAAG | 2 cut(s) | 290, 740 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 719 |
| BglII | AGATCT | 1 cut(s) | 630 |
| BisI | GCNGC | 7 cut(s) | 358, 516, 523, 539, 548, 671, 891 |
| BlsI | GCNGC | 7 cut(s) | 359, 517, 524, 540, 549, 672, 892 |
| Bme18I | GGWCC | 3 cut(s) | 39, 395, 590 |
| BmeT110I | CYCGRG | 5 cut(s) | 551, 586, 608, 626, 660 |
| BmgT120I | GGNCC | 6 cut(s) | 39, 395, 583, 584, 590, 848 |
| BmiI | GGNNCC | 9 cut(s) | 40, 55, 126, 584, 585, 591, 592, 646, 791 |
| BmsI | GCATC | 1 cut(s) | 828 |
| BoxI | GACNNNNGTC | 1 cut(s) | 172 |
| BpiI | GAAGAC | 1 cut(s) | 182 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 164, 196 |
| BpuEI | CTTGAG | 1 cut(s) | 131 |
| BsaHI | GRCGYC | 2 cut(s) | 54, 645 |
| BsaWI | WCCGGW | 2 cut(s) | 447, 806 |
| Bsc4I | CCNNNNNNNGG | 8 cut(s) | 48, 328, 721, 722, 758, 799, 806, 876 |
| Bse118I | RCCGGY | 4 cut(s) | 25, 362, 447, 824 |
| Bse3DI | GCAATG | 2 cut(s) | 375, 410 |
| BseGI | GGATG | 3 cut(s) | 386, 748, 812 |
| BseLI | CCNNNNNNNGG | 8 cut(s) | 48, 328, 721, 722, 758, 799, 806, 876 |
| BseMI | GCAATG | 2 cut(s) | 375, 410 |
| BsePI | GCGCGC | 2 cut(s) | 680, 776 |
| BseSI | GKGCMC | 4 cut(s) | 587, 602, 704, 792 |
| BseXI | GCAGC | 3 cut(s) | 369, 559, 902 |
| BseYI | CCCAGC | 1 cut(s) | 601 |
| BsgI | GTGCAG | 1 cut(s) | 619 |
| Bsh1236I | CGCG | 8 cut(s) | 325, 522, 541, 680, 682, 737, 778, 780 |
| Bsh1285I | CGRYCG | 2 cut(s) | 428, 504 |
| BshFI | GGCC | 3 cut(s) | 230, 585, 849 |
| BshNI | GGYRCC | 3 cut(s) | 53, 644, 789 |
| BshTI | ACCGGT | 1 cut(s) | 447 |
| BsiEI | CGRYCG | 2 cut(s) | 428, 504 |
| BsiHKAI | GWGCWC | 1 cut(s) | 862 |
| BsiHKCI | CYCGRG | 5 cut(s) | 551, 586, 608, 626, 660 |
| BslFI | GGGAC | 2 cut(s) | 493, 603 |
| BslI | CCNNNNNNNGG | 8 cut(s) | 48, 328, 721, 722, 758, 799, 806, 876 |
| BsmFI | GGGAC | 2 cut(s) | 493, 603 |
| BsnI | GGCC | 3 cut(s) | 230, 585, 849 |
| BsoBI | CYCGRG | 5 cut(s) | 551, 586, 608, 626, 660 |
| Bsp120I | GGGCCC | 1 cut(s) | 583 |
| Bsp1286I | GDGCHC | 6 cut(s) | 587, 602, 654, 704, 792, 862 |
| Bsp143I | GATC | 2 cut(s) | 15, 630 |
| BspANI | GGCC | 3 cut(s) | 230, 585, 849 |
| BspFNI | CGCG | 8 cut(s) | 325, 522, 541, 680, 682, 737, 778, 780 |
| BspHI | TCATGA | 1 cut(s) | 268 |
| BspLI | GGNNCC | 9 cut(s) | 40, 55, 126, 584, 585, 591, 592, 646, 791 |
| BspPI | GGATC | 1 cut(s) | 23 |
| BspT107I | GGYRCC | 3 cut(s) | 53, 644, 789 |
| BspTI | CTTAAG | 2 cut(s) | 290, 740 |
| BsrBI | CCGCTC | 1 cut(s) | 532 |
| BsrDI | GCAATG | 2 cut(s) | 375, 410 |
| BsrFI | RCCGGY | 4 cut(s) | 25, 362, 447, 824 |
| BssAI | RCCGGY | 4 cut(s) | 25, 362, 447, 824 |
| BssHII | GCGCGC | 2 cut(s) | 680, 776 |
| BssMI | GATC | 2 cut(s) | 15, 630 |
| BssNI | GRCGYC | 2 cut(s) | 54, 645 |
| BssT1I | CCWWGG | 2 cut(s) | 225, 870 |
| Bst4CI | ACNGT | 2 cut(s) | 426, 794 |
| BstACI | GRCGYC | 2 cut(s) | 54, 645 |
| BstAFI | CTTAAG | 2 cut(s) | 290, 740 |
| BstC8I | GCNNGC | 5 cut(s) | 23, 27, 520, 682, 778 |
| BstDSI | CCRYGG | 2 cut(s) | 231, 539 |
| BstF5I | GGATG | 3 cut(s) | 386, 748, 812 |
| BstFNI | CGCG | 8 cut(s) | 325, 522, 541, 680, 682, 737, 778, 780 |
| BstH2I | RGCGCY | 3 cut(s) | 57, 648, 748 |
| BstHHI | GCGC | 9 cut(s) | 56, 327, 647, 682, 684, 739, 747, 778, 780 |
| BstKTI | GATC | 2 cut(s) | 18, 633 |
| BstMBI | GATC | 2 cut(s) | 15, 630 |
| BstMCI | CGRYCG | 2 cut(s) | 428, 504 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 172 |
| BstSLI | GKGCMC | 4 cut(s) | 587, 602, 704, 792 |
| BstUI | CGCG | 8 cut(s) | 325, 522, 541, 680, 682, 737, 778, 780 |
| BstV1I | GCAGC | 3 cut(s) | 369, 559, 902 |
| BstV2I | GAAGAC | 1 cut(s) | 182 |
| BstX2I | RGATCY | 1 cut(s) | 630 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 845 |
| BstYI | RGATCY | 1 cut(s) | 630 |
| BsuRI | GGCC | 3 cut(s) | 230, 585, 849 |
| BtgI | CCRYGG | 2 cut(s) | 231, 539 |
| BtgZI | GCGATG | 2 cut(s) | 384, 805 |
| BtsCI | GGATG | 3 cut(s) | 386, 748, 812 |
| Cac8I | GCNNGC | 5 cut(s) | 23, 27, 520, 682, 778 |
| CciI | TCATGA | 1 cut(s) | 268 |
| CfoI | GCGC | 9 cut(s) | 56, 327, 647, 682, 684, 739, 747, 778, 780 |
| Cfr10I | RCCGGY | 4 cut(s) | 25, 362, 447, 824 |
| Cfr13I | GGNCC | 6 cut(s) | 39, 395, 583, 584, 590, 848 |
| Cfr42I | CCGCGG | 1 cut(s) | 542 |
| Cfr9I | CCCGGG | 2 cut(s) | 586, 660 |
| Csp6I | GTAC | 1 cut(s) | 445 |
| CspAI | ACCGGT | 1 cut(s) | 447 |
| CviAII | CATG | 1 cut(s) | 269 |
| CviQI | GTAC | 1 cut(s) | 445 |
| DinI | GGCGCC | 2 cut(s) | 55, 646 |
| DpnI | GATC | 2 cut(s) | 17, 632 |
| DpnII | GATC | 2 cut(s) | 15, 630 |
| DraI | TTTAAA | 1 cut(s) | 913 |
| EaeI | YGGCCR | 1 cut(s) | 228 |
| Ecl136II | GAGCTC | 1 cut(s) | 860 |
| Eco130I | CCWWGG | 2 cut(s) | 225, 870 |
| Eco24I | GRGCYC | 3 cut(s) | 587, 654, 862 |
| Eco47I | GGWCC | 3 cut(s) | 39, 395, 590 |
| Eco53kI | GAGCTC | 1 cut(s) | 860 |
| Eco88I | CYCGRG | 5 cut(s) | 551, 586, 608, 626, 660 |
| EcoICRI | GAGCTC | 1 cut(s) | 860 |
| EcoO109I | RGGNCCY | 2 cut(s) | 583, 590 |
| EcoT14I | CCWWGG | 2 cut(s) | 225, 870 |
| EcoT38I | GRGCYC | 3 cut(s) | 587, 654, 862 |
| EgeI | GGCGCC | 2 cut(s) | 55, 646 |
| EheI | GGCGCC | 2 cut(s) | 55, 646 |
| ErhI | CCWWGG | 2 cut(s) | 225, 870 |
| FaeI | CATG | 1 cut(s) | 272 |
| FaiI | YATR | 4 cut(s) | 135, 270, 302, 728 |
| FaqI | GGGAC | 2 cut(s) | 493, 603 |
| FatI | CATG | 1 cut(s) | 268 |
| FauI | CCCGC | 9 cut(s) | 30, 67, 207, 319, 330, 537, 685, 715, 824 |
| FblI | GTMKAC | 1 cut(s) | 250 |
| Fnu4HI | GCNGC | 7 cut(s) | 358, 516, 523, 539, 548, 671, 891 |
| FokI | GGATG | 3 cut(s) | 373, 735, 799 |
| FriOI | GRGCYC | 3 cut(s) | 587, 654, 862 |
| Fsp4HI | GCNGC | 7 cut(s) | 358, 516, 523, 539, 548, 671, 891 |
| FspBI | CTAG | 2 cut(s) | 170, 764 |
| GlaI | GCGC | 9 cut(s) | 55, 326, 646, 681, 683, 738, 746, 777, 779 |
| GluI | GCNGC | 7 cut(s) | 358, 516, 523, 539, 548, 671, 891 |
| GsaI | CCCAGC | 1 cut(s) | 605 |
| HaeII | RGCGCY | 3 cut(s) | 57, 648, 748 |
| HaeIII | GGCC | 3 cut(s) | 230, 585, 849 |
| HhaI | GCGC | 9 cut(s) | 56, 327, 647, 682, 684, 739, 747, 778, 780 |
| Hin1I | GRCGYC | 2 cut(s) | 54, 645 |
| Hin1II | CATG | 1 cut(s) | 272 |
| Hin6I | GCGC | 9 cut(s) | 54, 325, 645, 680, 682, 737, 745, 776, 778 |
| HinP1I | GCGC | 9 cut(s) | 54, 325, 645, 680, 682, 737, 745, 776, 778 |
| HincII | GTYRAC | 1 cut(s) | 384 |
| HindII | GTYRAC | 1 cut(s) | 384 |
| HpaI | GTTAAC | 1 cut(s) | 384 |
| HphI | GGTGA | 1 cut(s) | 553 |
| Hpy166II | GTNNAC | 4 cut(s) | 251, 319, 384, 561 |
| Hpy188I | TCNGA | 3 cut(s) | 67, 167, 430 |
| Hpy188III | TCNNGA | 3 cut(s) | 72, 269, 863 |
| Hpy8I | GTNNAC | 4 cut(s) | 251, 319, 384, 561 |
| Hpy99I | CGWCG | 3 cut(s) | 576, 644, 721 |
| HpyAV | CCTTC | 2 cut(s) | 115, 481 |
| HpyCH4III | ACNGT | 2 cut(s) | 426, 794 |
| HpyCH4IV | ACGT | 1 cut(s) | 89 |
| HpyCH4V | TGCA | 1 cut(s) | 636 |
| HpySE526I | ACGT | 1 cut(s) | 89 |
| Hsp92I | GRCGYC | 2 cut(s) | 54, 645 |
| Hsp92II | CATG | 1 cut(s) | 272 |
| HspAI | GCGC | 9 cut(s) | 54, 325, 645, 680, 682, 737, 745, 776, 778 |
| KasI | GGCGCC | 2 cut(s) | 53, 644 |
| KflI | GGGWCCC | 1 cut(s) | 590 |
| KroI | GCCGGC | 1 cut(s) | 25 |
| KroNI | GCCGGC | 1 cut(s) | 27 |
| KspAI | GTTAAC | 1 cut(s) | 384 |
| KspI | CCGCGG | 1 cut(s) | 542 |
| Kzo9I | GATC | 2 cut(s) | 15, 630 |
| LmnI | GCTCC | 3 cut(s) | 130, 490, 857 |
| Lsp1109I | GCAGC | 3 cut(s) | 369, 559, 902 |
| LweI | GCATC | 1 cut(s) | 828 |
| MaeI | CTAG | 2 cut(s) | 170, 764 |
| MaeII | ACGT | 1 cut(s) | 89 |
| MaeIII | GTNAC | 3 cut(s) | 304, 731, 794 |
| MalI | GATC | 2 cut(s) | 17, 632 |
| MbiI | CCGCTC | 1 cut(s) | 532 |
| MboI | GATC | 2 cut(s) | 15, 630 |
| MboII | GAAGA | 2 cut(s) | 182, 332 |
| MflI | RGATCY | 1 cut(s) | 630 |
| MhlI | GDGCHC | 6 cut(s) | 587, 602, 654, 704, 792, 862 |
| MluCI | AATT | 2 cut(s) | 335, 417 |
| Mly113I | GGCGCC | 2 cut(s) | 54, 645 |
| MlyI | GAGTC | 4 cut(s) | 182, 306, 464, 515 |
| MmeI | TCCRAC | 2 cut(s) | 145, 408 |
| MnlI | CCTC | 6 cut(s) | 42, 73, 153, 268, 617, 696 |
| MroNI | GCCGGC | 1 cut(s) | 25 |
| MroXI | GAANNNNTTC | 1 cut(s) | 339 |
| MseI | TTAA | 7 cut(s) | 92, 291, 383, 416, 420, 741, 912 |
| MslI | CAYNNNNRTG | 1 cut(s) | 403 |
| MspA1I | CMGCKG | 1 cut(s) | 541 |
| MspCI | CTTAAG | 2 cut(s) | 290, 740 |
| MvnI | CGCG | 8 cut(s) | 325, 522, 541, 680, 682, 737, 778, 780 |
| NaeI | GCCGGC | 1 cut(s) | 27 |
| NarI | GGCGCC | 2 cut(s) | 54, 645 |
| NdeII | GATC | 2 cut(s) | 15, 630 |
| NgoMIV | GCCGGC | 1 cut(s) | 25 |
| NlaIII | CATG | 1 cut(s) | 272 |
| NlaIV | GGNNCC | 9 cut(s) | 40, 55, 126, 584, 585, 591, 592, 646, 791 |
| NmuCI | GTSAC | 1 cut(s) | 731 |
| PacI | TTAATTAA | 1 cut(s) | 420 |
| PaeR7I | CTCGAG | 2 cut(s) | 551, 608 |
| PagI | TCATGA | 1 cut(s) | 268 |
| PauI | GCGCGC | 2 cut(s) | 680, 776 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 216 |
| PdiI | GCCGGC | 1 cut(s) | 27 |
| PdmI | GAANNNNTTC | 1 cut(s) | 339 |
| PfeI | GAWTC | 6 cut(s) | 68, 205, 263, 431, 770, 866 |
| PinAI | ACCGGT | 1 cut(s) | 447 |
| PkrI | GCNGC | 7 cut(s) | 359, 517, 524, 540, 549, 672, 892 |
| PleI | GAGTC | 4 cut(s) | 181, 305, 463, 514 |
| PluTI | GGCGCC | 2 cut(s) | 57, 648 |
| PpsI | GAGTC | 4 cut(s) | 181, 305, 463, 514 |
| PpuMI | RGGWCCY | 1 cut(s) | 590 |
| PshAI | GACNNNNGTC | 1 cut(s) | 172 |
| Psp124BI | GAGCTC | 1 cut(s) | 862 |
| Psp1406I | AACGTT | 1 cut(s) | 89 |
| Psp5II | RGGWCCY | 1 cut(s) | 590 |
| PspFI | CCCAGC | 1 cut(s) | 601 |
| PspN4I | GGNNCC | 9 cut(s) | 40, 55, 126, 584, 585, 591, 592, 646, 791 |
| PspOMI | GGGCCC | 1 cut(s) | 583 |
| PspPI | GGNCC | 6 cut(s) | 39, 395, 583, 584, 590, 848 |
| PspPPI | RGGWCCY | 1 cut(s) | 590 |
| PspXI | VCTCGAGB | 2 cut(s) | 551, 608 |
| PsuI | RGATCY | 1 cut(s) | 630 |
| PteI | GCGCGC | 2 cut(s) | 680, 776 |
| RsaI | GTAC | 1 cut(s) | 446 |
| RsaNI | GTAC | 1 cut(s) | 445 |
| RseI | CAYNNNNRTG | 1 cut(s) | 403 |
| SacI | GAGCTC | 1 cut(s) | 862 |
| SacII | CCGCGG | 1 cut(s) | 542 |
| SaqAI | TTAA | 7 cut(s) | 92, 291, 383, 416, 420, 741, 912 |
| SatI | GCNGC | 7 cut(s) | 358, 516, 523, 539, 548, 671, 891 |
| Sau3AI | GATC | 2 cut(s) | 15, 630 |
| Sau96I | GGNCC | 6 cut(s) | 39, 395, 583, 584, 590, 848 |
| SchI | GAGTC | 4 cut(s) | 182, 306, 464, 515 |
| SduI | GDGCHC | 6 cut(s) | 587, 602, 654, 704, 792, 862 |
| SetI | ASST | 9 cut(s) | 92, 145, 182, 190, 291, 552, 733, 862, 909 |
| SfaNI | GCATC | 1 cut(s) | 828 |
| SfoI | GGCGCC | 2 cut(s) | 55, 646 |
| Sfr274I | CTCGAG | 2 cut(s) | 551, 608 |
| Sfr303I | CCGCGG | 1 cut(s) | 542 |
| SgrAI | CRCCGGYG | 1 cut(s) | 362 |
| SgrBI | CCGCGG | 1 cut(s) | 542 |
| SinI | GGWCC | 3 cut(s) | 39, 395, 590 |
| SlaI | CTCGAG | 2 cut(s) | 551, 608 |
| SmaI | CCCGGG | 2 cut(s) | 588, 662 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 403 |
| SmlI | CTYRAG | 5 cut(s) | 146, 290, 551, 608, 740 |
| SmoI | CTYRAG | 5 cut(s) | 146, 290, 551, 608, 740 |
| Sse9I | AATT | 2 cut(s) | 335, 417 |
| SspDI | GGCGCC | 2 cut(s) | 53, 644 |
| SspMI | CTAG | 2 cut(s) | 170, 764 |
| SstI | GAGCTC | 1 cut(s) | 862 |
| StyI | CCWWGG | 2 cut(s) | 225, 870 |
| TaaI | ACNGT | 2 cut(s) | 426, 794 |
| TaiI | ACGT | 1 cut(s) | 92 |
| TaqI | TCGA | 5 cut(s) | 453, 504, 552, 609, 864 |
| TasI | AATT | 2 cut(s) | 335, 417 |
| TauI | GCSGC | 4 cut(s) | 518, 525, 541, 673 |
| TfiI | GAWTC | 6 cut(s) | 68, 205, 263, 431, 770, 866 |
| Tru1I | TTAA | 7 cut(s) | 92, 291, 383, 416, 420, 741, 912 |
| Tru9I | TTAA | 7 cut(s) | 92, 291, 383, 416, 420, 741, 912 |
| TseFI | GTSAC | 1 cut(s) | 731 |
| TseI | GCWGC | 3 cut(s) | 357, 547, 890 |
| Tsp45I | GTSAC | 1 cut(s) | 731 |
| TspDTI | ATGAA | 1 cut(s) | 801 |
| TspGWI | ACGGA | 1 cut(s) | 432 |
| TspMI | CCCGGG | 2 cut(s) | 586, 660 |
| Vha464I | CTTAAG | 2 cut(s) | 290, 740 |
| VpaK11BI | GGWCC | 3 cut(s) | 39, 395, 590 |
| XapI | RAATTY | 1 cut(s) | 335 |
| XhoI | CTCGAG | 2 cut(s) | 551, 608 |
| XmaI | CCCGGG | 2 cut(s) | 586, 660 |
| XmiI | GTMKAC | 1 cut(s) | 250 |
| XmnI | GAANNNNTTC | 1 cut(s) | 339 |
| XspI | CTAG | 2 cut(s) | 170, 764 |