Rroxscaffold_17G00435870

mitotic sister chromatid biorientation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000017
OG0000098
Physical Location & Seq
Forward (+)
412108 .. 414794
2687 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_17G00435870.1

Sequence Viewer

Length: 1854 bp
ATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCTAGCTTTAAATTCCAAAGGTCTAAAGGATCGATAGGCCACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATTTCTGTTCTCGTTGAGCTCATCTTAGGACACCTGCGTTATCTTTTAACAGATGTGCCGCCCCAGCCAAACTCCCCACCTGACAATGTCTTCCGCCCGGATCAGCCCGCCGAAGCAGGCTTTGGGTCCAAAAAGAGGGGCAGTGCCCCGCCTCCGATTCACGGAATAAGTAAAATAACGTTAAAAGTAGTGGTATTTCACTTTCGCCCGAAGGCTCCCACTTATACTACACCTCTCAAGTCATTTCACAAAGTCGGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGACAGGGACAGTGGGAATCTCTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTGTTGAACGCCCGGGGAAAGCCCCCGAAGGAGCGTTCCCAGTCCGTCCCCCGGCCGGCACGCGGCGACCCGCTCTCGCCGCGGAAGCAGCTCGAGCAGTTCACCGACAGCCGACGGGTTCGGGACTGGGACCCCGTGCCCAGCCCTCAGAGCCAATCCTTTTCCCGAGGTTACGGATCCATTTTGCCGACTTCCCTTGCCTACATTGTTCCATTGACCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCGTGAATGGCACTCGGTCCTCCGGATTTTCAAGGGCCGCCGGGGGCGCACCGGACACCACGCGACGTGCGGTGCTCTTCCAGCCGCTGGACCCTACCTCCGGCTGAGCCGTTTCCAGGGTGGGCAGGCTATCTGCACCGACGGCCGCTCCGCCGGCTCACGCCCCAGGTTTTGCAGCGACCGCCGCGCCCTCCTACTCATCGGGGCCTGGCACTTGCCCCGACGGCCGGGTATAGGTCACGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGACGAACGATTTGCACGTCAGTATCGCTGCGGGCCTCCACCAGAGTTTCCTCTGGGCTTCGCCCCGCTCAGGCATAGTTCACCATCTTTCGGGTCCCGACAGGCATGCTCACACTCGAACCCTTCTCAGAAGATCAAGGTCGGTCGGCGGTGCACCCGCAAAGGGATCCCGCACATTAGCTTCCTTGCGCCTTACGGGTTTAATCACCCGTTGACTCGCACACATGTCAGACTCCTTGGTCCGTGTTTCAAGACGGGCCGAATGGGGAGCCCGCAGGCCGTTACCAGGAGCACGCAGATGCCGAAGCACGCCGAGACGGCGCATGCTGCCTACCATGATCGCGTCGACGACGTCTCCACGGGCATATCAACAGCCCGGGCTTTGGCCGCCGCCGCAATCCGTAACGGTCCACGCCCCGAGTCGAGTGGCGGACCGGCTTGTGACCGTTCCACATCCGACCGAGGCGCATCGCCGGCCCCCATCCGCTTCCCTCCCGACAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTCCCTCGCGGTACTTGTTTGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAACAACCCGACTCGCCGACAGCGCCTCGTGGTGCGACAGGGTCCGGCACAACGGGGCTCTCACCCTCTATGGCGCCCCCTTCCAGGGGACTTGTTCCCGGTCCGCCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

617

Amino Acids

67.66

Weight (kDa)

11.12

Isoelectric Point (pI)

55.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000098)

Species Orthologous Gene IDs
pyrus_communis pycom05g23460 pycom581g00100 pycom849g00040
rosa_multiflora Rmu_sc0019961.1_g000001
rosa_roxburghii Rroxscaffold_100G00451180 Rroxscaffold_102G00450890 Rroxscaffold_102G00450920 Rroxscaffold_103G00450530 Rroxscaffold_103G00450560 Rroxscaffold_104G00444830 Rroxscaffold_104G00444900 Rroxscaffold_105G00447100 Rroxscaffold_105G00447180 Rroxscaffold_107G00442470 Rroxscaffold_107G00442480 Rroxscaffold_107G00442520 Rroxscaffold_107G00442540 Rroxscaffold_107G00442560 Rroxscaffold_107G00442570 Rroxscaffold_107G00442600 Rroxscaffold_107G00442630 Rroxscaffold_107G00442650 Rroxscaffold_107G00442660 Rroxscaffold_108G00451480 Rroxscaffold_110G00451510 Rroxscaffold_111G00451550 Rroxscaffold_112G00451590 Rroxscaffold_113G00451630 Rroxscaffold_114G00451650 Rroxscaffold_116G00451710 Rroxscaffold_117G00451740 Rroxscaffold_117G00451750 Rroxscaffold_121G00451840 Rroxscaffold_122G00451860 Rroxscaffold_122G00451880 Rroxscaffold_123G00451920 Rroxscaffold_124G00451950 Rroxscaffold_128G00452040 Rroxscaffold_128G00452060 Rroxscaffold_12G00450600 Rroxscaffold_12G00450620 Rroxscaffold_131G00452110 Rroxscaffold_138G00452180 Rroxscaffold_13G00448630 Rroxscaffold_145G00452240 Rroxscaffold_14G00441930 Rroxscaffold_14G00441950 Rroxscaffold_14G00442050 Rroxscaffold_14G00442080 Rroxscaffold_15G00447500 Rroxscaffold_162G00450770 Rroxscaffold_16G00446200 Rroxscaffold_16G00446210 Rroxscaffold_16G00446240 Rroxscaffold_17G00435360 Rroxscaffold_17G00435410 Rroxscaffold_17G00435450 Rroxscaffold_17G00435480 Rroxscaffold_17G00435500 Rroxscaffold_17G00435600 Rroxscaffold_17G00435660 Rroxscaffold_17G00435680 Rroxscaffold_17G00435760 Rroxscaffold_17G00435780 Rroxscaffold_17G00435810 Rroxscaffold_17G00435840 Rroxscaffold_17G00435870 Rroxscaffold_18G00446030 Rroxscaffold_18G00446060 Rroxscaffold_18G00446130 Rroxscaffold_19G00448890 Rroxscaffold_19G00448920 Rroxscaffold_19G00448940 Rroxscaffold_19G00448970 Rroxscaffold_1G00000020 Rroxscaffold_1G00000050 Rroxscaffold_1G00000160 Rroxscaffold_20G00445060 Rroxscaffold_20G00445100 Rroxscaffold_20G00445130 Rroxscaffold_21G00439490 Rroxscaffold_21G00439560 Rroxscaffold_21G00439580 Rroxscaffold_21G00439620 Rroxscaffold_21G00439630 Rroxscaffold_22G00439910 Rroxscaffold_22G00439950 Rroxscaffold_23G00451020 Rroxscaffold_23G00451040 Rroxscaffold_23G00451070 Rroxscaffold_24G00444920 Rroxscaffold_24G00444950 Rroxscaffold_24G00445000 Rroxscaffold_25G00450200 Rroxscaffold_26G00447730 Rroxscaffold_27G00446570 Rroxscaffold_27G00446600 Rroxscaffold_28G00446830 Rroxscaffold_28G00446860 Rroxscaffold_28G00446910 Rroxscaffold_29G00441560 Rroxscaffold_29G00441570 Rroxscaffold_29G00441590 Rroxscaffold_29G00441630 Rroxscaffold_29G00441640 Rroxscaffold_29G00441670 Rroxscaffold_29G00441680 Rroxscaffold_29G00441860 Rroxscaffold_30G00449540 Rroxscaffold_30G00449570 Rroxscaffold_30G00449580 Rroxscaffold_31G00438120 Rroxscaffold_31G00438140 Rroxscaffold_31G00438180 Rroxscaffold_31G00438190 Rroxscaffold_31G00438220 Rroxscaffold_31G00438230 Rroxscaffold_32G00442740 Rroxscaffold_32G00442760 Rroxscaffold_33G00439670 Rroxscaffold_33G00439720 Rroxscaffold_33G00439740 Rroxscaffold_33G00439760 Rroxscaffold_33G00439820 Rroxscaffold_34G00443140 Rroxscaffold_34G00443200 Rroxscaffold_34G00443250 Rroxscaffold_34G00443370 Rroxscaffold_34G00443390 Rroxscaffold_35G00441050 Rroxscaffold_35G00441080 Rroxscaffold_35G00441090 Rroxscaffold_35G00441170 Rroxscaffold_35G00441190 Rroxscaffold_35G00441230 Rroxscaffold_36G00440100 Rroxscaffold_36G00440170 Rroxscaffold_37G00445170 Rroxscaffold_38G00444440 Rroxscaffold_38G00444500 Rroxscaffold_38G00444550 Rroxscaffold_39G00448200 Rroxscaffold_39G00448210 Rroxscaffold_40G00447650 Rroxscaffold_40G00447680 Rroxscaffold_40G00447700 Rroxscaffold_40G00447710 Rroxscaffold_41G00451110 Rroxscaffold_41G00451130 Rroxscaffold_42G00450430 Rroxscaffold_43G00449800 Rroxscaffold_43G00449860 Rroxscaffold_43G00449920 Rroxscaffold_43G00449930 Rroxscaffold_44G00440540 Rroxscaffold_44G00440580 Rroxscaffold_44G00440660 Rroxscaffold_45G00438900 Rroxscaffold_45G00438930 Rroxscaffold_45G00438950 Rroxscaffold_47G00444020 Rroxscaffold_47G00444060 Rroxscaffold_47G00444120 Rroxscaffold_48G00448450 Rroxscaffold_49G00438570 Rroxscaffold_49G00438650 Rroxscaffold_49G00438700 Rroxscaffold_4G00321080 Rroxscaffold_50G00444130 Rroxscaffold_50G00444200 Rroxscaffold_50G00444210 Rroxscaffold_50G00444330 Rroxscaffold_50G00444350 Rroxscaffold_51G00447840 Rroxscaffold_52G00439310 Rroxscaffold_52G00439390 Rroxscaffold_54G00443660 Rroxscaffold_55G00450020 Rroxscaffold_55G00450050 Rroxscaffold_55G00450060 Rroxscaffold_55G00450080 Rroxscaffold_55G00450090 Rroxscaffold_55G00450120 Rroxscaffold_57G00443070 Rroxscaffold_59G00442210 Rroxscaffold_59G00442220 Rroxscaffold_60G00448360 Rroxscaffold_60G00448370 Rroxscaffold_60G00448410 Rroxscaffold_61G00449990 Rroxscaffold_62G00438040 Rroxscaffold_62G00438060 Rroxscaffold_62G00438080 Rroxscaffold_63G00444410 Rroxscaffold_63G00444430 Rroxscaffold_64G00450670 Rroxscaffold_65G00445300 Rroxscaffold_65G00445340 Rroxscaffold_65G00445360 Rroxscaffold_65G00445380 Rroxscaffold_65G00445420 Rroxscaffold_66G00437660 Rroxscaffold_66G00437820 Rroxscaffold_66G00437840 Rroxscaffold_66G00437890 Rroxscaffold_67G00448140 Rroxscaffold_67G00448150 Rroxscaffold_68G00447010 Rroxscaffold_69G00446670 Rroxscaffold_69G00446710 Rroxscaffold_69G00446770 Rroxscaffold_6G00387820 Rroxscaffold_70G00446300 Rroxscaffold_70G00446340 Rroxscaffold_70G00446380 Rroxscaffold_71G00445590 Rroxscaffold_71G00445600 Rroxscaffold_71G00445630 Rroxscaffold_71G00445690 Rroxscaffold_71G00445720 Rroxscaffold_71G00445740 Rroxscaffold_71G00445770 Rroxscaffold_72G00449370 Rroxscaffold_72G00449410 Rroxscaffold_72G00449450 Rroxscaffold_73G00439000 Rroxscaffold_73G00439060 Rroxscaffold_73G00439160 Rroxscaffold_73G00439270 Rroxscaffold_74G00442920 Rroxscaffold_75G00447260 Rroxscaffold_75G00447290 Rroxscaffold_75G00447430 Rroxscaffold_76G00448540 Rroxscaffold_76G00448610 Rroxscaffold_77G00449150 Rroxscaffold_78G00449610 Rroxscaffold_79G00450210 Rroxscaffold_80G00450330 Rroxscaffold_80G00450350 Rroxscaffold_81G00450480 Rroxscaffold_83G00450980 Rroxscaffold_85G00451230 Rroxscaffold_86G00451270 Rroxscaffold_89G00451410 Rroxscaffold_90G00448760 Rroxscaffold_90G00448770 Rroxscaffold_90G00448820 Rroxscaffold_91G00443000 Rroxscaffold_91G00443060 Rroxscaffold_92G00446430 Rroxscaffold_93G00440830 Rroxscaffold_93G00440850 Rroxscaffold_93G00440860 Rroxscaffold_93G00440880 Rroxscaffold_93G00440910 Rroxscaffold_94G00445830 Rroxscaffold_94G00445910 Rroxscaffold_94G00445940 Rroxscaffold_94G00446020 Rroxscaffold_97G00442340 Rroxscaffold_97G00442390 Rroxscaffold_97G00442400 Rroxscaffold_98G00451350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 207
AasI GACNNNNNNGTC 1 cut(s) 1350
AatII GACGTC 1 cut(s) 1467
Acc36I ACCTGC 1 cut(s) 207
AccB1I GGYRCC 1 cut(s) 1815
AccB7I CCANNNNNTGG 1 cut(s) 917
AccBSI CCGCTC 3 cut(s) 637, 978, 1180
AccI GTMKAC 3 cut(s) 505, 559, 1458
AccII CGCG 7 cut(s) 627, 646, 893, 1017, 1073, 1455, 1665
AccIII TCCGGA 1 cut(s) 852
AclI AACGTT 1 cut(s) 344
AclWI GGATC 6 cut(s) 91, 273, 735, 748, 1273, 1286
AcoI YGGCCR 4 cut(s) 617, 973, 1055, 1497
AcsI RAATTY 2 cut(s) 65, 1713
AcyI GRCGYC 2 cut(s) 1464, 1816
AfaI GTAC 4 cut(s) 118, 546, 823, 1669
AflII CTTAAG 1 cut(s) 1076
AflIII ACRYGT 1 cut(s) 1336
AgsI TTSAA 5 cut(s) 571, 862, 1363, 1618, 1641
AjiI CACGTC 2 cut(s) 897, 1130
AjnI CCWGG 5 cut(s) 945, 995, 1037, 1397, 1825
AjuI GAANNNNNNNTTGG 2 cut(s) 270, 302
AluBI AGCT 6 cut(s) 60, 141, 184, 435, 655, 1293
AluI AGCT 6 cut(s) 60, 141, 184, 435, 655, 1293
Alw21I GWGCWC 4 cut(s) 186, 907, 1268, 1406
Alw26I GTCTC 4 cut(s) 796, 1421, 1471, 1690
Alw44I GTGCAC 1 cut(s) 1264
AlwI GGATC 6 cut(s) 91, 273, 735, 748, 1273, 1286
AlwNI CAGNNNCTG 2 cut(s) 789, 917
Ama87I CYCGRG 5 cut(s) 576, 656, 729, 1488, 1529
Aor13HI TCCGGA 1 cut(s) 852
ApaLI GTGCAC 1 cut(s) 1264
ApeKI GCWGC 6 cut(s) 13, 652, 1005, 1140, 1439, 1733
ApoI RAATTY 2 cut(s) 65, 1713
ArsI GACNNNNNNTTYG 2 cut(s) 1106, 1138
AspLEI GCGC 9 cut(s) 879, 1019, 1075, 1083, 1303, 1435, 1580, 1767, 1818
AsuHPI GGTGA 5 cut(s) 658, 786, 1185, 1310, 1796
AvaI CYCGRG 5 cut(s) 576, 656, 729, 1488, 1529
BaeGI GKGCMC 3 cut(s) 313, 705, 1268
BaeI ACNNNNGTAYC 2 cut(s) 1118, 1151
BamHI GGATCC 2 cut(s) 740, 1278
BanI GGYRCC 1 cut(s) 1815
BanII GRGCYC 3 cut(s) 186, 1385, 1802
BauI CACGAG 2 cut(s) 161, 1769
BbsI GAAGAC 2 cut(s) 247, 438
Bbv12I GWGCWC 4 cut(s) 186, 907, 1268, 1406
BbvI GCAGC 5 cut(s) 664, 1017, 1127, 1426, 1720
BccI CCATC 3 cut(s) 1092, 1204, 1601
BceAI ACGGC 5 cut(s) 924, 988, 1070, 1376, 1446
BcgI CGANNNNNNTGC 4 cut(s) 1106, 1140, 1200, 1234
BciT130I CCWGG 5 cut(s) 947, 997, 1039, 1399, 1827
BcoDI GTCTC 4 cut(s) 796, 1421, 1471, 1690
BfaI CTAG 3 cut(s) 57, 425, 1100
BfoI RGCGCY 3 cut(s) 1084, 1768, 1819
BfrI CTTAAG 1 cut(s) 1076
BfuAI ACCTGC 1 cut(s) 207
BglI GCCNNNNNGGC 2 cut(s) 1054, 1430
BlpI GCTNAGC 1 cut(s) 935
BmeT110I CYCGRG 5 cut(s) 576, 656, 729, 1488, 1529
BmgBI CACGTC 2 cut(s) 897, 1130
BmrI ACTGGG 2 cut(s) 598, 700
BmsI GCATC 3 cut(s) 799, 1401, 1589
BmuI ACTGGG 2 cut(s) 598, 700
BoxI GACNNNNGTC 1 cut(s) 427
BpiI GAAGAC 2 cut(s) 247, 438
BplI GAGNNNNNCTC 2 cut(s) 419, 451
Bpu10I CCTNAGC 1 cut(s) 1181
Bpu1102I GCTNAGC 1 cut(s) 935
BpuEI CTTGAG 1 cut(s) 386
Bsa29I ATCGAT 1 cut(s) 86
BsaHI GRCGYC 2 cut(s) 1464, 1816
BsaI GGTCTC 2 cut(s) 796, 1690
BsaWI WCCGGW 2 cut(s) 852, 881
BsaXI ACNNNNNCTCC 6 cut(s) 912, 942, 962, 992, 1451, 1481
Bse118I RCCGGY 4 cut(s) 619, 983, 1546, 1585
Bse1I ACTGG 5 cut(s) 24, 124, 548, 604, 695
BseAI TCCGGA 1 cut(s) 852
BseBI CCWGG 5 cut(s) 947, 997, 1039, 1399, 1827
BseCI ATCGAT 1 cut(s) 86
BseGI GGATG 3 cut(s) 1084, 1565, 1593
BseMII CTCAG 5 cut(s) 545, 725, 926, 1195, 1253
BseNI ACTGG 5 cut(s) 24, 124, 548, 604, 695
BseSI GKGCMC 3 cut(s) 313, 705, 1268
BseX3I CGGCCG 3 cut(s) 617, 973, 1055
BseXI GCAGC 5 cut(s) 664, 1017, 1127, 1426, 1720
BseYI CCCAGC 2 cut(s) 228, 704
BsgI GTGCAG 1 cut(s) 949
Bsh1236I CGCG 7 cut(s) 627, 646, 893, 1017, 1073, 1455, 1665
Bsh1285I CGRYCG 7 cut(s) 620, 828, 976, 1012, 1058, 1258, 1573
BshNI GGYRCC 1 cut(s) 1815
BshVI ATCGAT 1 cut(s) 86
BsiEI CGRYCG 7 cut(s) 620, 828, 976, 1012, 1058, 1258, 1573
BsiHKAI GWGCWC 4 cut(s) 186, 907, 1268, 1406
BsiHKCI CYCGRG 5 cut(s) 576, 656, 729, 1488, 1529
BslFI GGGAC 6 cut(s) 525, 596, 701, 707, 1192, 1844
BsmAI GTCTC 4 cut(s) 796, 1421, 1471, 1690
BsmBI CGTCTC 2 cut(s) 1421, 1471
BsmFI GGGAC 6 cut(s) 525, 596, 701, 707, 1192, 1844
BsmI GAATGC 1 cut(s) 1736
Bso31I GGTCTC 2 cut(s) 796, 1690
BsoBI CYCGRG 5 cut(s) 576, 656, 729, 1488, 1529
Bsp1286I GDGCHC 8 cut(s) 186, 313, 705, 907, 1268, 1385, 1406, 1802
Bsp13I TCCGGA 1 cut(s) 852
Bsp143I GATC 6 cut(s) 83, 265, 740, 1245, 1278, 1450
Bsp1720I GCTNAGC 1 cut(s) 935
BspCNI CTCAG 5 cut(s) 546, 724, 927, 1194, 1252
BspDI ATCGAT 1 cut(s) 86
BspEI TCCGGA 1 cut(s) 852
BspFNI CGCG 7 cut(s) 627, 646, 893, 1017, 1073, 1455, 1665
BspMI ACCTGC 1 cut(s) 207
BspPI GGATC 6 cut(s) 91, 273, 735, 748, 1273, 1286
BspQI GCTCTTC 1 cut(s) 912
BspT107I GGYRCC 1 cut(s) 1815
BspTI CTTAAG 1 cut(s) 1076
BspTNI GGTCTC 2 cut(s) 796, 1690
BsrBI CCGCTC 3 cut(s) 637, 978, 1180
BsrFI RCCGGY 4 cut(s) 619, 983, 1546, 1585
BsrI ACTGG 5 cut(s) 24, 124, 548, 604, 695
BssAI RCCGGY 4 cut(s) 619, 983, 1546, 1585
BssMI GATC 6 cut(s) 83, 265, 740, 1245, 1278, 1450
BssNI GRCGYC 2 cut(s) 1464, 1816
BssSI CACGAG 2 cut(s) 161, 1769
BssT1I CCWWGG 4 cut(s) 480, 797, 1348, 1704
Bst2BI CACGAG 2 cut(s) 161, 1769
Bst2UI CCWGG 5 cut(s) 947, 997, 1039, 1399, 1827
Bst4CI ACNGT 5 cut(s) 106, 516, 564, 1520, 1559
Bst6I CTCTTC 1 cut(s) 912
BstACI GRCGYC 2 cut(s) 1464, 1816
BstAFI CTTAAG 1 cut(s) 1076
BstDEI CTNAG 6 cut(s) 190, 554, 711, 935, 1181, 1239
BstDSI CCRYGG 2 cut(s) 644, 1470
BstF5I GGATG 3 cut(s) 1084, 1565, 1593
BstFNI CGCG 7 cut(s) 627, 646, 893, 1017, 1073, 1455, 1665
BstH2I RGCGCY 3 cut(s) 1084, 1768, 1819
BstHHI GCGC 9 cut(s) 879, 1019, 1075, 1083, 1303, 1435, 1580, 1767, 1818
BstKTI GATC 6 cut(s) 86, 268, 743, 1248, 1281, 1453
BstMAI GTCTC 4 cut(s) 796, 1421, 1471, 1690
BstMBI GATC 6 cut(s) 83, 265, 740, 1245, 1278, 1450
BstMCI CGRYCG 7 cut(s) 620, 828, 976, 1012, 1058, 1258, 1573
BstNI CCWGG 5 cut(s) 947, 997, 1039, 1399, 1827
BstNSI RCATGY 3 cut(s) 1221, 1340, 1439
BstPAI GACNNNNGTC 1 cut(s) 427
BstSLI GKGCMC 3 cut(s) 313, 705, 1268
BstUI CGCG 7 cut(s) 627, 646, 893, 1017, 1073, 1455, 1665
BstV1I GCAGC 5 cut(s) 664, 1017, 1127, 1426, 1720
BstV2I GAAGAC 2 cut(s) 247, 438
BstX2I RGATCY 2 cut(s) 740, 1278
BstYI RGATCY 2 cut(s) 740, 1278
BstZI CGGCCG 3 cut(s) 617, 973, 1055
Bsu15I ATCGAT 1 cut(s) 86
BsuTUI ATCGAT 1 cut(s) 86
BtgI CCRYGG 2 cut(s) 644, 1470
BtgZI GCGATG 1 cut(s) 1566
BtrI CACGTC 2 cut(s) 897, 1130
BtsCI GGATG 3 cut(s) 1084, 1565, 1593
BtsI GCAGTG 1 cut(s) 313
BtsIMutI CAGTG 2 cut(s) 313, 521
BveI ACCTGC 1 cut(s) 207
CaiI CAGNNNCTG 2 cut(s) 789, 917
CfoI GCGC 9 cut(s) 879, 1019, 1075, 1083, 1303, 1435, 1580, 1767, 1818
Cfr10I RCCGGY 4 cut(s) 619, 983, 1546, 1585
Cfr42I CCGCGG 1 cut(s) 647
Cfr9I CCCGGG 2 cut(s) 576, 1488
ClaI ATCGAT 1 cut(s) 86
CpoI CGGWCCG 2 cut(s) 1544, 1843
CseI GACGC 1 cut(s) 1444
Csp6I GTAC 4 cut(s) 117, 545, 822, 1668
CspI CGGWCCG 2 cut(s) 1544, 1843
CviAII CATG 4 cut(s) 1218, 1337, 1436, 1448
CviQI GTAC 4 cut(s) 117, 545, 822, 1668
DdeI CTNAG 6 cut(s) 190, 554, 711, 935, 1181, 1239
DinI GGCGCC 1 cut(s) 1817
DpnI GATC 6 cut(s) 85, 267, 742, 1247, 1280, 1452
DpnII GATC 6 cut(s) 83, 265, 740, 1245, 1278, 1450
DraI TTTAAA 1 cut(s) 64
DrdI GACNNNNNNGTC 1 cut(s) 1350
DseDI GACNNNNNNGTC 1 cut(s) 1350
EaeI YGGCCR 4 cut(s) 617, 973, 1055, 1497
EagI CGGCCG 3 cut(s) 617, 973, 1055
Eam1104I CTCTTC 1 cut(s) 912
EarI CTCTTC 1 cut(s) 912
EciI GGCGGA 4 cut(s) 248, 970, 1557, 1835
Ecl136II GAGCTC 1 cut(s) 184
EclXI CGGCCG 3 cut(s) 617, 973, 1055
Eco130I CCWWGG 4 cut(s) 480, 797, 1348, 1704
Eco24I GRGCYC 3 cut(s) 186, 1385, 1802
Eco31I GGTCTC 2 cut(s) 796, 1690
Eco52I CGGCCG 3 cut(s) 617, 973, 1055
Eco53kI GAGCTC 1 cut(s) 184
Eco88I CYCGRG 5 cut(s) 576, 656, 729, 1488, 1529
EcoICRI GAGCTC 1 cut(s) 184
EcoO109I RGGNCCY 3 cut(s) 694, 1035, 1206
EcoRII CCWGG 5 cut(s) 945, 995, 1037, 1397, 1825
EcoT14I CCWWGG 4 cut(s) 480, 797, 1348, 1704
EcoT38I GRGCYC 3 cut(s) 186, 1385, 1802
EgeI GGCGCC 1 cut(s) 1817
EheI GGCGCC 1 cut(s) 1817
ErhI CCWWGG 4 cut(s) 480, 797, 1348, 1704
Esp3I CGTCTC 2 cut(s) 1421, 1471
FaeI CATG 4 cut(s) 1221, 1340, 1439, 1451
FaqI GGGAC 6 cut(s) 525, 596, 701, 707, 1192, 1844
FatI CATG 4 cut(s) 1217, 1336, 1435, 1447
FauI CCCGC 9 cut(s) 280, 321, 463, 642, 1136, 1185, 1277, 1290, 1392
FblI GTMKAC 3 cut(s) 505, 559, 1458
FokI GGATG 3 cut(s) 1071, 1552, 1580
FriOI GRGCYC 3 cut(s) 186, 1385, 1802
FspBI CTAG 3 cut(s) 57, 425, 1100
GlaI GCGC 9 cut(s) 878, 1018, 1074, 1082, 1302, 1434, 1579, 1766, 1817
GsaI CCCAGC 2 cut(s) 232, 708
HaeII RGCGCY 3 cut(s) 1084, 1768, 1819
HgaI GACGC 1 cut(s) 1444
HhaI GCGC 9 cut(s) 879, 1019, 1075, 1083, 1303, 1435, 1580, 1767, 1818
Hin1I GRCGYC 2 cut(s) 1464, 1816
Hin1II CATG 4 cut(s) 1221, 1340, 1439, 1451
Hin6I GCGC 9 cut(s) 877, 1017, 1073, 1081, 1301, 1433, 1578, 1765, 1816
HinP1I GCGC 9 cut(s) 877, 1017, 1073, 1081, 1301, 1433, 1578, 1765, 1816
HincII GTYRAC 3 cut(s) 560, 1326, 1459
HindII GTYRAC 3 cut(s) 560, 1326, 1459
HphI GGTGA 5 cut(s) 658, 786, 1185, 1310, 1796
Hpy166II GTNNAC 9 cut(s) 506, 560, 666, 794, 1193, 1266, 1326, 1459, 1523
Hpy188III TCNNGA 6 cut(s) 686, 729, 853, 1209, 1363, 1607
Hpy8I GTNNAC 9 cut(s) 506, 560, 666, 794, 1193, 1266, 1326, 1459, 1523
Hpy99I CGWCG 7 cut(s) 681, 898, 974, 1056, 1460, 1463, 1466
HpyAV CCTTC 4 cut(s) 370, 586, 1245, 1832
HpyCH4III ACNGT 5 cut(s) 106, 516, 564, 1520, 1559
HpyCH4IV ACGT 4 cut(s) 344, 896, 1129, 1464
HpyCH4V TGCA 5 cut(s) 966, 1005, 1127, 1266, 1736
HpyF3I CTNAG 6 cut(s) 190, 554, 711, 935, 1181, 1239
HpySE526I ACGT 4 cut(s) 344, 896, 1129, 1464
Hsp92I GRCGYC 2 cut(s) 1464, 1816
Hsp92II CATG 4 cut(s) 1221, 1340, 1439, 1451
HspAI GCGC 9 cut(s) 877, 1017, 1073, 1081, 1301, 1433, 1578, 1765, 1816
KasI GGCGCC 1 cut(s) 1815
KflI GGGWCCC 2 cut(s) 694, 1206
Kpn2I TCCGGA 1 cut(s) 852
KroI GCCGGC 3 cut(s) 619, 983, 1585
KroNI GCCGGC 3 cut(s) 621, 985, 1587
KspI CCGCGG 1 cut(s) 647
Kzo9I GATC 6 cut(s) 83, 265, 740, 1245, 1278, 1450
LguI GCTCTTC 1 cut(s) 912
LmnI GCTCC 5 cut(s) 385, 595, 983, 1380, 1401
Lsp1109I GCAGC 5 cut(s) 664, 1017, 1127, 1426, 1720
LweI GCATC 3 cut(s) 799, 1401, 1589
MaeI CTAG 3 cut(s) 57, 425, 1100
MaeII ACGT 4 cut(s) 344, 896, 1129, 1464
MaeIII GTNAC 6 cut(s) 38, 734, 1067, 1393, 1514, 1553
MalI GATC 6 cut(s) 85, 267, 742, 1247, 1280, 1452
MbiI CCGCTC 3 cut(s) 637, 978, 1180
MboI GATC 6 cut(s) 83, 265, 740, 1245, 1278, 1450
MboII GAAGA 4 cut(s) 247, 438, 899, 1255
MflI RGATCY 2 cut(s) 740, 1278
MhlI GDGCHC 8 cut(s) 186, 313, 705, 907, 1268, 1385, 1406, 1802
MluCI AATT 3 cut(s) 65, 1612, 1713
Mly113I GGCGCC 1 cut(s) 1816
MlyI GAGTC 7 cut(s) 437, 565, 1321, 1338, 1541, 1624, 1747
MmeI TCCRAC 2 cut(s) 400, 1593
MroI TCCGGA 1 cut(s) 852
MroNI GCCGGC 3 cut(s) 619, 983, 1585
MseI TTAA 5 cut(s) 63, 212, 347, 1077, 1314
MslI CAYNNNNRTG 1 cut(s) 1409
MspA1I CMGCKG 3 cut(s) 458, 646, 917
MspCI CTTAAG 1 cut(s) 1076
Mva1269I GAATGC 1 cut(s) 1736
MvaI CCWGG 5 cut(s) 947, 997, 1039, 1399, 1827
MvnI CGCG 7 cut(s) 627, 646, 893, 1017, 1073, 1455, 1665
NaeI GCCGGC 3 cut(s) 621, 985, 1587
NarI GGCGCC 1 cut(s) 1816
NdeII GATC 6 cut(s) 83, 265, 740, 1245, 1278, 1450
NgoMIV GCCGGC 3 cut(s) 619, 983, 1585
NlaIII CATG 4 cut(s) 1221, 1340, 1439, 1451
NmeAIII GCCGAG 1 cut(s) 1450
NmuCI GTSAC 2 cut(s) 1067, 1553
NspI RCATGY 3 cut(s) 1221, 1340, 1439
PaeI GCATGC 2 cut(s) 1221, 1439
PaeR7I CTCGAG 1 cut(s) 656
PaqCI CACCTGC 1 cut(s) 207
PciI ACATGT 1 cut(s) 1336
PciSI GCTCTTC 1 cut(s) 912
PcsI WCGNNNNNNNCGW 2 cut(s) 1126, 1412
PctI GAATGC 1 cut(s) 1736
PdiI GCCGGC 3 cut(s) 621, 985, 1587
PfeI GAWTC 6 cut(s) 130, 322, 461, 521, 531, 1106
PflFI GACNNNGTC 3 cut(s) 251, 1463, 1782
PflMI CCANNNNNTGG 1 cut(s) 917
PleI GAGTC 7 cut(s) 436, 564, 1321, 1338, 1540, 1624, 1747
PluTI GGCGCC 1 cut(s) 1819
PpsI GAGTC 7 cut(s) 436, 564, 1321, 1338, 1540, 1624, 1747
PpuMI RGGWCCY 2 cut(s) 694, 1206
PscI ACATGT 1 cut(s) 1336
PshAI GACNNNNGTC 1 cut(s) 427
Psp124BI GAGCTC 1 cut(s) 186
Psp1406I AACGTT 1 cut(s) 344
Psp5II RGGWCCY 2 cut(s) 694, 1206
Psp6I CCWGG 5 cut(s) 945, 995, 1037, 1397, 1825
PspFI CCCAGC 2 cut(s) 228, 704
PspGI CCWGG 5 cut(s) 945, 995, 1037, 1397, 1825
PspPPI RGGWCCY 2 cut(s) 694, 1206
PspXI VCTCGAGB 1 cut(s) 656
PstNI CAGNNNCTG 2 cut(s) 789, 917
PsuI RGATCY 2 cut(s) 740, 1278
PsyI GACNNNGTC 3 cut(s) 251, 1463, 1782
RsaI GTAC 4 cut(s) 118, 546, 823, 1669
RsaNI GTAC 4 cut(s) 117, 545, 822, 1668
RseI CAYNNNNRTG 1 cut(s) 1409
Rsr2I CGGWCCG 2 cut(s) 1544, 1843
RsrII CGGWCCG 2 cut(s) 1544, 1843
SacI GAGCTC 1 cut(s) 186
SacII CCGCGG 1 cut(s) 647
SalI GTCGAC 2 cut(s) 558, 1457
SapI GCTCTTC 1 cut(s) 912
SaqAI TTAA 5 cut(s) 63, 212, 347, 1077, 1314
Sau3AI GATC 6 cut(s) 83, 265, 740, 1245, 1278, 1450
SchI GAGTC 7 cut(s) 437, 565, 1321, 1338, 1541, 1624, 1747
SduI GDGCHC 8 cut(s) 186, 313, 705, 907, 1268, 1385, 1406, 1802
SfaNI GCATC 3 cut(s) 799, 1401, 1589
SfoI GGCGCC 1 cut(s) 1817
Sfr274I CTCGAG 1 cut(s) 656
Sfr303I CCGCGG 1 cut(s) 647
SgrBI CCGCGG 1 cut(s) 647
SgrDI CGTCGACG 1 cut(s) 1457
SlaI CTCGAG 1 cut(s) 656
SmaI CCCGGG 2 cut(s) 578, 1490
SmiMI CAYNNNNRTG 1 cut(s) 1409
SmlI CTYRAG 3 cut(s) 401, 656, 1076
SmoI CTYRAG 3 cut(s) 401, 656, 1076
SphI GCATGC 2 cut(s) 1221, 1439
SrfI GCCCGGGC 1 cut(s) 1490
Sse9I AATT 3 cut(s) 65, 1612, 1713
SspDI GGCGCC 1 cut(s) 1815
SspMI CTAG 3 cut(s) 57, 425, 1100
SstI GAGCTC 1 cut(s) 186
StyI CCWWGG 4 cut(s) 480, 797, 1348, 1704
TaaI ACNGT 5 cut(s) 106, 516, 564, 1520, 1559
TaiI ACGT 4 cut(s) 347, 899, 1132, 1467
TaqI TCGA 6 cut(s) 86, 559, 657, 1229, 1458, 1535
TaqII GACCGA 4 cut(s) 835, 1244, 1587, 1672
TasI AATT 3 cut(s) 65, 1612, 1713
TfiI GAWTC 6 cut(s) 130, 322, 461, 521, 531, 1106
Tru1I TTAA 5 cut(s) 63, 212, 347, 1077, 1314
Tru9I TTAA 5 cut(s) 63, 212, 347, 1077, 1314
TscAI CASTG 2 cut(s) 313, 521
TseFI GTSAC 2 cut(s) 1067, 1553
TseI GCWGC 6 cut(s) 13, 652, 1005, 1140, 1439, 1733
Tsp45I GTSAC 2 cut(s) 1067, 1553
TspDTI ATGAA 2 cut(s) 17, 1641
TspGWI ACGGA 7 cut(s) 342, 532, 598, 753, 1344, 1502, 1725
TspMI CCCGGG 2 cut(s) 576, 1488
TspRI CASTG 2 cut(s) 313, 521
Tth111I GACNNNGTC 3 cut(s) 251, 1463, 1782
Van91I CCANNNNNTGG 1 cut(s) 917
Vha464I CTTAAG 1 cut(s) 1076
VneI GTGCAC 1 cut(s) 1264
XapI RAATTY 2 cut(s) 65, 1713
XceI RCATGY 3 cut(s) 1221, 1340, 1439
XhoI CTCGAG 1 cut(s) 656
XmaI CCCGGG 2 cut(s) 576, 1488
XmiI GTMKAC 3 cut(s) 505, 559, 1458
XspI CTAG 3 cut(s) 57, 425, 1100
ZraI GACGTC 1 cut(s) 1465
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.