MD12G1050800.v1.1

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000234
Physical Location & Seq
Forward (+)
5752524 .. 5753583
1060 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1050800.v1.1.491

Sequence Viewer

Length: 975 bp
ATGTTTAATCACAATATACCCGAAGGAATCTTGTTTGATATCCTTGCAAGATTGCCAGTGAGATCTCTTTTGCGGTTTAGATGCGTTTGTAAATCTTGGAAAAGTTTAATTGGCAGCTCTAGTTTCAAAAGTGCCCATCTCGAAAGGAACGTCGTGCAAAGTTCTTGTGATTTTCTCCTTATCCAAACAGATGACAAGAAAAATTGTTTGTCACTGTTTGATGCTGAAACATCGTCCAAGTGTTTGGATGTAGAGCCTCCTGCGGATAAATTAGTCTCAGATTCTTTGTTCATGAAAGCTCAAGAGGCTTCCTATAATGGCCTCGCTCAGCACACAAGCCATTTTGTTAATGGTTTCCGGTTTCATAGTGGGGAAAACGACTATAAGGTTGTGAGGCTTATTAGGGTTTTGCTTCCACACGAGAGTGTCGTCCTTGAAGTTGAGGTTTACAGCCTTAGATCAGATGCTTGGAGAAGAATAACTGTGATGCCTTATTTTTCAAATACTGTCATTTCTTGGCAATCAAGATGTACCTTCTTTGATGGAGTTGTGTATTGGATTATAACGGAGCATTCTCACACTTCCATCCTTGCTTTCGATTTGGGCAGTGAGGTTTTTCGAAAGATTGTGATACCACCCGAATTTATAGGTCATATTTTTATTCTAGTGTTTGAGAAATCACTCTCTTTGTTCCATACAAGACCTGGTCATGCATATGGGTTCTGGAAGCGGATGCTTAATTGGAAAGTTATCCGGACGATTTGTCTTCCTGATGGTACTAGATTTGTAGCCTGGCCATTGGGAATTAGAAAAAATCTTGGCAAAGTTCACATGATTCAGGATGGAAGAAAACCAAAGCTTGTTTTGTATGATCCCGAGTCACACCAAGTTAAAGATACTGGAATCAGAACGGAGTCCAGTTACTTTCACGATGTTGATGCTTATTGTCAGGGCCTATTTTTACTCGACTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1

Protein Analysis

325

Amino Acids

37.42

Weight (kDa)

8.56

Isoelectric Point (pI)

38.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 5 - 43 6.6e-11 F-box domain
F-box-like PF12937 5 - 38 8.2e-08 F-box-like
FBA_1 PF07734 111 - 214 9.1e-12 F-box associated beta propeller domain
FBA_3 PF08268 122 - 215 1.2e-07 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000234)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18880 FvH4_2g02020 FvH4_2g02020 FvH4_2g37110 FvH4_6g33750 FvH4_6g33770 FvH4_6g34412 FvH4_7g08973
malus_domestica MD09G1191300.v1.1 MD09G1281000.v1.1 MD09G1281100.v1.1 MD09G1281200.v1.1 MD12G1050800.v1.1
prunus_persica Prupe.1G188300_v2.0.a1 Prupe.2G120700_v2.0.a1 Prupe.3G013300_v2.0.a1 Prupe.3G013500_v2.0.a1 Prupe.3G047100_v2.0.a1 Prupe.3G047200_v2.0.a1 Prupe.3G047300_v2.0.a1 Prupe.3G047400_v2.0.a1 Prupe.6G319100_v2.0.a1
pyrus_communis pycom09g18870 pycom11g17570 pycom12g04520 pycom14g04100
rosa_chinensis RchiOBHm_Chr1g0321721 RchiOBHm_Chr1g0321751 RchiOBHm_Chr1g0344471 RchiOBHm_Chr1g0344741 RchiOBHm_Chr1g0344891 RchiOBHm_Chr2g0143631 RchiOBHm_Chr2g0143641 RchiOBHm_Chr2g0144781 RchiOBHm_Chr3g0458821 RchiOBHm_Chr6g0245251 RchiOBHm_Chr6g0304681
rosa_laevigata RLG00000007681 RLG00000010222 RLG00000013320 RLG00000015313 RLG00000017431 RLG00000017439 RLG00000020055 RLG00000020056 RLG00000020112 RLG00000025133 RLG00000028872 RLG00000028881 RLG00000028884 RLG00000030407
rosa_multiflora Rmu_co8324891.1_g000001 Rmu_sc0000087.1_g000002 Rmu_sc0001288.1_g000009 Rmu_sc0001634.1_g000031 Rmu_sc0001942.1_g000032 Rmu_sc0003410.1_g000036 Rmu_sc0003909.1_g000002 Rmu_sc0004340.1_g000024 Rmu_sc0005308.1_g000008 Rmu_sc0005705.1_g000040 Rmu_sc0016560.1_g000002 Rmu_ssc0000167.1_g000005
rosa_roxburghii Rroxscaffold_2G00101580 Rroxscaffold_4G00309600 Rroxscaffold_4G00309710 Rroxscaffold_4G00327490 Rroxscaffold_5G00333360 Rroxscaffold_6G00420770 Rroxscaffold_7G00163600 Rroxscaffold_7G00215330
rosa_rugosa Rorug01G0174400 Rorug02G0213500 Rorug02G0384500 Rorug02G0384500 Rorug02G0384500 Rorug03G0032100 Rorug03G0301100 Rorug05G0513100
rosa_samantha Rh1AG045800 Rh1AG046600 Rh1AG191000 Rh1AG191200 Rh1AG192400 Rh1BG045100 Rh1BG157400 Rh1BG158200 Rh1CG048800 Rh1CG176300 Rh1CG177700 Rh1DG053100 Rh1DG055000 Rh1DG188800 Rh2AG180100 Rh2AG180200 Rh2AG436400 Rh2AG436500 Rh2AG441300 Rh2BG444000 Rh2BG444100 Rh2BG452000 Rh2CG422700 Rh2CG422800 Rh2CG428600 Rh2DG185900 Rh2DG186000 Rh2DG454300 Rh2DG454400 Rh2DG461700 Rh3AG089700 Rh3BG092600 Rh3CG092900 Rh3DG093600 Rh4AG005900 Rh4DG004900 Rh4DG010300 Rh4DG010400 Rh4DG230700 Rh6AG028300 Rh6AG445500 Rh6BG024500 Rh6BG456400 Rh6BG456500 Rh6CG022600 Rh6CG458300 Rh6CG458400 Rh6DG022600 Rh6DG445500
rosa_wichuraiana Rw1G004020 Rw1G004060 Rw1G004180 Rw1G015690 Rw1G015790 Rw2G014090 Rw2G035570 Rw2G035580 Rw2G036090 Rw3G007680 Rw6G002380 Rw6G038740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 563
AccIII TCCGGA 1 cut(s) 753
AciI CCGC 3 cut(s) 73, 263, 730
AclWI GGATC 1 cut(s) 866
AcoI YGGCCR 1 cut(s) 794
AcsI RAATTY 1 cut(s) 641
AfaI GTAC 2 cut(s) 532, 778
AgsI TTSAA 3 cut(s) 127, 437, 501
AhdI GACNNNNNGTC 1 cut(s) 762
AjnI CCWGG 2 cut(s) 703, 791
AleI CACNNNNGTG 1 cut(s) 423
AluBI AGCT 3 cut(s) 117, 299, 859
AluI AGCT 3 cut(s) 117, 299, 859
Alw26I GTCTC 1 cut(s) 280
AlwI GGATC 1 cut(s) 866
Ama87I CYCGRG 1 cut(s) 875
Aor13HI TCCGGA 1 cut(s) 753
AoxI GGCC 3 cut(s) 319, 794, 952
ApeKI GCWGC 1 cut(s) 114
ApoI RAATTY 1 cut(s) 641
ArsI GACNNNNNNTTYG 2 cut(s) 135, 167
AspS9I GGNCC 1 cut(s) 952
AsuII TTCGAA 1 cut(s) 619
AvaI CYCGRG 1 cut(s) 875
BaeGI GKGCMC 1 cut(s) 136
BalI TGGCCA 1 cut(s) 796
BauI CACGAG 1 cut(s) 419
BbsI GAAGAC 1 cut(s) 758
BbvI GCAGC 1 cut(s) 126
BccI CCATC 5 cut(s) 144, 536, 593, 767, 836
BcgI CGANNNNNNTGC 2 cut(s) 920, 954
BciT130I CCWGG 2 cut(s) 705, 793
BcoDI GTCTC 1 cut(s) 280
BfaI CTAG 3 cut(s) 120, 665, 780
BglII AGATCT 1 cut(s) 62
BisI GCNGC 1 cut(s) 115
BlpI GCTNAGC 1 cut(s) 327
BlsI GCNGC 1 cut(s) 116
Bme1390I CCNGG 2 cut(s) 705, 793
BmeRI GACNNNNNGTC 1 cut(s) 762
BmeT110I CYCGRG 1 cut(s) 875
BmgT120I GGNCC 1 cut(s) 952
BmrFI CCNGG 2 cut(s) 705, 793
BmsI GCATC 6 cut(s) 71, 211, 454, 477, 723, 928
BpiI GAAGAC 1 cut(s) 758
Bpu1102I GCTNAGC 1 cut(s) 327
Bpu14I TTCGAA 1 cut(s) 619
BpuEI CTTGAG 1 cut(s) 285
BsaWI WCCGGW 2 cut(s) 357, 753
Bse1I ACTGG 4 cut(s) 56, 904, 918, 974
BseAI TCCGGA 1 cut(s) 753
BseBI CCWGG 2 cut(s) 705, 793
BseGI GGATG 4 cut(s) 253, 585, 738, 847
BseMII CTCAG 2 cut(s) 291, 341
BseNI ACTGG 4 cut(s) 56, 904, 918, 974
BseSI GKGCMC 1 cut(s) 136
BseXI GCAGC 1 cut(s) 126
BshFI GGCC 3 cut(s) 321, 796, 954
BsiHKCI CYCGRG 1 cut(s) 875
BsiSI CCGG 2 cut(s) 358, 754
BsmAI GTCTC 1 cut(s) 280
BsmI GAATGC 1 cut(s) 571
BsnI GGCC 3 cut(s) 321, 796, 954
BsoBI CYCGRG 1 cut(s) 875
Bsp119I TTCGAA 1 cut(s) 619
Bsp1286I GDGCHC 1 cut(s) 136
Bsp13I TCCGGA 1 cut(s) 753
Bsp143I GATC 3 cut(s) 62, 458, 871
Bsp1720I GCTNAGC 1 cut(s) 327
BspACI CCGC 3 cut(s) 73, 263, 730
BspANI GGCC 3 cut(s) 321, 796, 954
BspCNI CTCAG 2 cut(s) 290, 340
BspEI TCCGGA 1 cut(s) 753
BspHI TCATGA 1 cut(s) 291
BspPI GGATC 1 cut(s) 866
BspT104I TTCGAA 1 cut(s) 619
BsrI ACTGG 4 cut(s) 56, 904, 918, 974
BssMI GATC 3 cut(s) 62, 458, 871
BssSI CACGAG 1 cut(s) 419
Bst2BI CACGAG 1 cut(s) 419
Bst2UI CCWGG 2 cut(s) 705, 793
Bst4CI ACNGT 3 cut(s) 216, 484, 508
BstBI TTCGAA 1 cut(s) 619
BstDEI CTNAG 3 cut(s) 277, 327, 455
BstF5I GGATG 4 cut(s) 253, 585, 738, 847
BstKTI GATC 3 cut(s) 65, 461, 874
BstMAI GTCTC 1 cut(s) 280
BstMBI GATC 3 cut(s) 62, 458, 871
BstMWI GCNNNNNNNGC 1 cut(s) 305
BstNI CCWGG 2 cut(s) 705, 793
BstSCI CCNGG 2 cut(s) 703, 791
BstSLI GKGCMC 1 cut(s) 136
BstV1I GCAGC 1 cut(s) 126
BstV2I GAAGAC 1 cut(s) 758
BstX2I RGATCY 1 cut(s) 62
BstXI CCANNNNNNTGG 1 cut(s) 244
BstYI RGATCY 1 cut(s) 62
BsuRI GGCC 3 cut(s) 321, 796, 954
BtsCI GGATG 4 cut(s) 253, 585, 738, 847
BtsI GCAGTG 1 cut(s) 613
BtsIMutI CAGTG 3 cut(s) 63, 212, 613
CciI TCATGA 1 cut(s) 291
Cfr13I GGNCC 1 cut(s) 952
CsiI ACCWGGT 1 cut(s) 703
Csp6I GTAC 2 cut(s) 531, 777
CviAII CATG 3 cut(s) 292, 710, 832
CviQI GTAC 2 cut(s) 531, 777
DdeI CTNAG 3 cut(s) 277, 327, 455
DpnI GATC 3 cut(s) 64, 460, 873
DpnII GATC 3 cut(s) 62, 458, 871
DriI GACNNNNNGTC 1 cut(s) 762
EaeI YGGCCR 1 cut(s) 794
Eam1105I GACNNNNNGTC 1 cut(s) 762
Eco32I GATATC 1 cut(s) 40
Eco88I CYCGRG 1 cut(s) 875
EcoO109I RGGNCCY 1 cut(s) 952
EcoRII CCWGG 2 cut(s) 703, 791
EcoRV GATATC 1 cut(s) 40
EcoT22I ATGCAT 1 cut(s) 715
FaeI CATG 3 cut(s) 295, 713, 835
FatI CATG 3 cut(s) 291, 709, 831
FauNDI CATATG 1 cut(s) 715
Fnu4HI GCNGC 1 cut(s) 115
FokI GGATG 4 cut(s) 260, 572, 745, 854
Fsp4HI GCNGC 1 cut(s) 115
FspBI CTAG 3 cut(s) 120, 665, 780
GluI GCNGC 1 cut(s) 115
HaeIII GGCC 3 cut(s) 321, 796, 954
HapII CCGG 2 cut(s) 358, 754
Hin1II CATG 3 cut(s) 295, 713, 835
HindIII AAGCTT 1 cut(s) 857
HinfI GANTC 6 cut(s) 27, 281, 835, 878, 903, 914
HpaII CCGG 2 cut(s) 358, 754
Hpy166II GTNNAC 2 cut(s) 448, 829
Hpy188I TCNGA 3 cut(s) 280, 463, 908
Hpy8I GTNNAC 2 cut(s) 448, 829
Hpy99I CGWCG 1 cut(s) 155
HpyAV CCTTC 2 cut(s) 17, 544
HpyCH4III ACNGT 3 cut(s) 216, 484, 508
HpyCH4IV ACGT 1 cut(s) 150
HpyCH4V TGCA 3 cut(s) 47, 157, 713
HpyF10VI GCNNNNNNNGC 1 cut(s) 305
HpyF3I CTNAG 3 cut(s) 277, 327, 455
HpySE526I ACGT 1 cut(s) 150
Hsp92II CATG 3 cut(s) 295, 713, 835
Kpn2I TCCGGA 1 cut(s) 753
Kzo9I GATC 3 cut(s) 62, 458, 871
LmnI GCTCC 1 cut(s) 568
Lsp1109I GCAGC 1 cut(s) 126
LweI GCATC 6 cut(s) 71, 211, 454, 477, 723, 928
MabI ACCWGGT 1 cut(s) 703
MaeI CTAG 3 cut(s) 120, 665, 780
MaeII ACGT 1 cut(s) 150
MaeIII GTNAC 3 cut(s) 210, 879, 920
MalI GATC 3 cut(s) 64, 460, 873
MboI GATC 3 cut(s) 62, 458, 871
MboII GAAGA 3 cut(s) 486, 758, 858
MflI RGATCY 1 cut(s) 62
MhlI GDGCHC 1 cut(s) 136
MlsI TGGCCA 1 cut(s) 796
MluCI AATT 6 cut(s) 108, 202, 269, 641, 739, 804
MluNI TGGCCA 1 cut(s) 796
MlyI GAGTC 2 cut(s) 887, 923
MnlI CCTC 6 cut(s) 267, 298, 332, 387, 436, 604
Mox20I TGGCCA 1 cut(s) 796
Mph1103I ATGCAT 1 cut(s) 715
MroI TCCGGA 1 cut(s) 753
MscI TGGCCA 1 cut(s) 796
MseI TTAA 5 cut(s) 6, 107, 348, 738, 891
MslI CAYNNNNRTG 2 cut(s) 423, 714
Msp20I TGGCCA 1 cut(s) 796
MspI CCGG 2 cut(s) 358, 754
MspR9I CCNGG 2 cut(s) 705, 793
Mva1269I GAATGC 1 cut(s) 571
MvaI CCWGG 2 cut(s) 705, 793
MwoI GCNNNNNNNGC 1 cut(s) 305
NdeI CATATG 1 cut(s) 715
NdeII GATC 3 cut(s) 62, 458, 871
NlaIII CATG 3 cut(s) 295, 713, 835
NmuCI GTSAC 2 cut(s) 210, 879
NsiI ATGCAT 1 cut(s) 715
NspV TTCGAA 1 cut(s) 619
OliI CACNNNNGTG 1 cut(s) 423
PagI TCATGA 1 cut(s) 291
PcsI WCGNNNNNNNCGW 2 cut(s) 147, 426
PctI GAATGC 1 cut(s) 571
PfeI GAWTC 4 cut(s) 27, 281, 835, 903
PflFI GACNNNGTC 1 cut(s) 705
PkrI GCNGC 1 cut(s) 116
PleI GAGTC 2 cut(s) 886, 922
PpsI GAGTC 2 cut(s) 886, 922
PsiI TTATAA 1 cut(s) 563
Psp6I CCWGG 2 cut(s) 703, 791
PspGI CCWGG 2 cut(s) 703, 791
PspPI GGNCC 1 cut(s) 952
PsuI RGATCY 1 cut(s) 62
PsyI GACNNNGTC 1 cut(s) 705
RsaI GTAC 2 cut(s) 532, 778
RsaNI GTAC 2 cut(s) 531, 777
RseI CAYNNNNRTG 2 cut(s) 423, 714
SaqAI TTAA 5 cut(s) 6, 107, 348, 738, 891
SatI GCNGC 1 cut(s) 115
Sau3AI GATC 3 cut(s) 62, 458, 871
Sau96I GGNCC 1 cut(s) 952
SchI GAGTC 2 cut(s) 887, 923
ScrFI CCNGG 2 cut(s) 705, 793
SduI GDGCHC 1 cut(s) 136
SexAI ACCWGGT 1 cut(s) 703
SfaNI GCATC 6 cut(s) 71, 211, 454, 477, 723, 928
SfuI TTCGAA 1 cut(s) 619
SmiMI CAYNNNNRTG 2 cut(s) 423, 714
SmlI CTYRAG 1 cut(s) 300
SmoI CTYRAG 1 cut(s) 300
Sse9I AATT 6 cut(s) 108, 202, 269, 641, 739, 804
SsiI CCGC 3 cut(s) 73, 263, 730
SspMI CTAG 3 cut(s) 120, 665, 780
StyD4I CCNGG 2 cut(s) 703, 791
TaaI ACNGT 3 cut(s) 216, 484, 508
TaiI ACGT 1 cut(s) 153
TaqI TCGA 4 cut(s) 141, 597, 619, 966
TasI AATT 6 cut(s) 108, 202, 269, 641, 739, 804
TfiI GAWTC 4 cut(s) 27, 281, 835, 903
Tru1I TTAA 5 cut(s) 6, 107, 348, 738, 891
Tru9I TTAA 5 cut(s) 6, 107, 348, 738, 891
TscAI CASTG 3 cut(s) 63, 219, 613
TseFI GTSAC 2 cut(s) 210, 879
TseI GCWGC 1 cut(s) 114
Tsp45I GTSAC 2 cut(s) 210, 879
TspDTI ATGAA 3 cut(s) 280, 308, 353
TspGWI ACGGA 2 cut(s) 581, 926
TspRI CASTG 3 cut(s) 63, 219, 613
Tth111I GACNNNGTC 1 cut(s) 705
XapI RAATTY 1 cut(s) 641
XcmI CCANNNNNNNNNTGG 2 cut(s) 347, 701
XspI CTAG 3 cut(s) 120, 665, 780
Zsp2I ATGCAT 1 cut(s) 715
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.