Rroxscaffold_4G00309710

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000234
Physical Location & Seq
Reverse (-)
31499197 .. 31500273
1077 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00309710.1

Sequence Viewer

Length: 1077 bp
ATGTTTGAAATCCTCACAAGATTATCAGTGAAATCTTTACTGAGATTCAGATGTGTTTGCAAATCTTGGAACTCATTAATTACCAGTCCAAATTTCATAAACAACCATCTTGAAAGACATGATATGGATAGTTCTTGTGACTATTTACTTGTCCAGACTGGTAGTGCAAAGTCACTCTTTTGTGCAAAAACATTTGTCAAGTATATGGACTTAGATCTTAGCAGTTGCGCAATGCATCTCGGTTTCAATGACTTCTTTGTTTATGGTTCGTGCAATGGATTGCTGTGCATCTGTGCTAACCATGGGCCTTTGAACAGTTCCATGTATATATGGAATCCATCGAACAGAAAAATCAAGAGACTTCCACAAGGTCTTTACCAAAATGTGGGTATTAATGTCAGTCTCGGGTTTGGATTTCACCGTCAGAAAAACGACTATAAAGTTGTGAAGATTGTACGCGGGGAAATTATGTACAAAGTTGAGGTCTACTCCCTTAGATTGAATTCATGGAGAGAACTTGGTGCAGTTCTCCCCATTTGGTCCTACTCTACATACCTAAACAAATCTGCAATACATGTCAATGGAGTTGTGTACTGGATGGTAGAGGAGAAAAACTCTGCAAGATCTTTCATACTTTCTTTTGATATGGACAATGAGGTTTTCCAAAAGATGGAACTCCCTGAGAAACTAGTCGGAGGGATTGGTTCTGTTAGTATCCAAGTGTTTGAGAAATCCCTTTCTTTGATACACTTGAGAGAAGATGAGGATAATCTTGTAAGGCATGTTTCTTACTGTGACATCTGGGTTATGGGACTGGAAACATGGAAAGTGATTCGCACGATTCTTCTACCGCAAGTGAGACTTTATCCAGCATATGGACGTATGGCATGGCCATTGAGCTTTACAACAGATGGAGTTTATATTGTAAGGCTGGATGAAAAAGAGTTCCAAACTTTGGTTTTATATGATCCTATATCGCAAGAAGCTAAGGCAATAACTAGAGTTAAACCGGGTTTTTATGGCTACATATACATGGAGGTCCACGCTTACAAAGAAACTCTAACTTTACTCGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1

Protein Analysis

358

Amino Acids

41.41

Weight (kDa)

8.44

Isoelectric Point (pI)

35.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 2 - 33 2.3e-09 F-box domain
F-box-like PF12937 2 - 30 4.9e-06 F-box-like
FBA_3 PF08268 78 - 328 6.9e-19 F-box associated beta propeller domain
FBA_1 PF07734 90 - 270 5.3e-23 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000234)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18880 FvH4_2g02020 FvH4_2g02020 FvH4_2g37110 FvH4_6g33750 FvH4_6g33770 FvH4_6g34412 FvH4_7g08973
malus_domestica MD09G1191300.v1.1 MD09G1281000.v1.1 MD09G1281100.v1.1 MD09G1281200.v1.1 MD12G1050800.v1.1
prunus_persica Prupe.1G188300_v2.0.a1 Prupe.2G120700_v2.0.a1 Prupe.3G013300_v2.0.a1 Prupe.3G013500_v2.0.a1 Prupe.3G047100_v2.0.a1 Prupe.3G047200_v2.0.a1 Prupe.3G047300_v2.0.a1 Prupe.3G047400_v2.0.a1 Prupe.6G319100_v2.0.a1
pyrus_communis pycom09g18870 pycom11g17570 pycom12g04520 pycom14g04100
rosa_chinensis RchiOBHm_Chr1g0321721 RchiOBHm_Chr1g0321751 RchiOBHm_Chr1g0344471 RchiOBHm_Chr1g0344741 RchiOBHm_Chr1g0344891 RchiOBHm_Chr2g0143631 RchiOBHm_Chr2g0143641 RchiOBHm_Chr2g0144781 RchiOBHm_Chr3g0458821 RchiOBHm_Chr6g0245251 RchiOBHm_Chr6g0304681
rosa_laevigata RLG00000007681 RLG00000010222 RLG00000013320 RLG00000015313 RLG00000017431 RLG00000017439 RLG00000020055 RLG00000020056 RLG00000020112 RLG00000025133 RLG00000028872 RLG00000028881 RLG00000028884 RLG00000030407
rosa_multiflora Rmu_co8324891.1_g000001 Rmu_sc0000087.1_g000002 Rmu_sc0001288.1_g000009 Rmu_sc0001634.1_g000031 Rmu_sc0001942.1_g000032 Rmu_sc0003410.1_g000036 Rmu_sc0003909.1_g000002 Rmu_sc0004340.1_g000024 Rmu_sc0005308.1_g000008 Rmu_sc0005705.1_g000040 Rmu_sc0016560.1_g000002 Rmu_ssc0000167.1_g000005
rosa_roxburghii Rroxscaffold_2G00101580 Rroxscaffold_4G00309600 Rroxscaffold_4G00309710 Rroxscaffold_4G00327490 Rroxscaffold_5G00333360 Rroxscaffold_6G00420770 Rroxscaffold_7G00163600 Rroxscaffold_7G00215330
rosa_rugosa Rorug01G0174400 Rorug02G0213500 Rorug02G0384500 Rorug02G0384500 Rorug02G0384500 Rorug03G0032100 Rorug03G0301100 Rorug05G0513100
rosa_samantha Rh1AG045800 Rh1AG046600 Rh1AG191000 Rh1AG191200 Rh1AG192400 Rh1BG045100 Rh1BG157400 Rh1BG158200 Rh1CG048800 Rh1CG176300 Rh1CG177700 Rh1DG053100 Rh1DG055000 Rh1DG188800 Rh2AG180100 Rh2AG180200 Rh2AG436400 Rh2AG436500 Rh2AG441300 Rh2BG444000 Rh2BG444100 Rh2BG452000 Rh2CG422700 Rh2CG422800 Rh2CG428600 Rh2DG185900 Rh2DG186000 Rh2DG454300 Rh2DG454400 Rh2DG461700 Rh3AG089700 Rh3BG092600 Rh3CG092900 Rh3DG093600 Rh4AG005900 Rh4DG004900 Rh4DG010300 Rh4DG010400 Rh4DG230700 Rh6AG028300 Rh6AG445500 Rh6BG024500 Rh6BG456400 Rh6BG456500 Rh6CG022600 Rh6CG458300 Rh6CG458400 Rh6DG022600 Rh6DG445500
rosa_wichuraiana Rw1G004020 Rw1G004060 Rw1G004180 Rw1G015690 Rw1G015790 Rw2G014090 Rw2G035570 Rw2G035580 Rw2G036090 Rw3G007680 Rw6G002380 Rw6G038740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 229
AccB7I CCANNNNNTGG 4 cut(s) 385, 670, 875, 955
AccI GTMKAC 1 cut(s) 486
AccII CGCG 1 cut(s) 459
AciI CCGC 2 cut(s) 459, 851
AclWI GGATC 1 cut(s) 962
AcoI YGGCCR 1 cut(s) 890
AcsI RAATTY 2 cut(s) 91, 502
AfaI GTAC 3 cut(s) 456, 473, 593
AfiI CCNNNNNNNGG 4 cut(s) 385, 670, 875, 955
AflIII ACRYGT 1 cut(s) 574
AgsI TTSAA 5 cut(s) 8, 113, 247, 313, 502
AhlI ACTAGT 1 cut(s) 688
AluBI AGCT 2 cut(s) 900, 986
AluI AGCT 2 cut(s) 900, 986
Alw26I GTCTC 3 cut(s) 352, 407, 853
AlwI GGATC 1 cut(s) 962
Ama87I CYCGRG 1 cut(s) 404
AoxI GGCC 2 cut(s) 305, 890
ApoI RAATTY 2 cut(s) 91, 502
ArsI GACNNNNNNTTYG 2 cut(s) 468, 500
AseI ATTAAT 2 cut(s) 77, 393
AspLEI GCGC 1 cut(s) 230
AspS9I GGNCC 3 cut(s) 305, 540, 1039
AsuC2I CCSGG 1 cut(s) 1011
AsuHPI GGTGA 1 cut(s) 410
AvaI CYCGRG 1 cut(s) 404
AvaII GGWCC 2 cut(s) 540, 1039
BalI TGGCCA 1 cut(s) 892
BccI CCATC 5 cut(s) 114, 346, 592, 664, 905
BciVI GTATCC 1 cut(s) 725
BcnI CCSGG 1 cut(s) 1011
BcoDI GTCTC 3 cut(s) 352, 407, 853
BcuI ACTAGT 1 cut(s) 688
BfaI CTAG 2 cut(s) 689, 999
BfuI GTATCC 1 cut(s) 725
BglII AGATCT 2 cut(s) 214, 623
Bme1390I CCNGG 1 cut(s) 1011
Bme18I GGWCC 2 cut(s) 540, 1039
BmeT110I CYCGRG 1 cut(s) 404
BmgT120I GGNCC 3 cut(s) 305, 540, 1039
BmrFI CCNGG 1 cut(s) 1011
BmsI GCATC 2 cut(s) 244, 297
BplI GAGNNNNNCTC 4 cut(s) 473, 505, 599, 631
Bpu10I CCTNAGC 1 cut(s) 987
BpuEI CTTGAG 1 cut(s) 772
BpuMI CCSGG 1 cut(s) 1011
BsaJI CCNNGG 1 cut(s) 301
Bsc4I CCNNNNNNNGG 4 cut(s) 385, 670, 875, 955
Bse1I ACTGG 4 cut(s) 84, 163, 599, 819
Bse3DI GCAATG 2 cut(s) 237, 280
BseDI CCNNGG 1 cut(s) 301
BseGI GGATG 2 cut(s) 603, 940
BseLI CCNNNNNNNGG 4 cut(s) 385, 670, 875, 955
BseMI GCAATG 2 cut(s) 237, 280
BseMII CTCAG 2 cut(s) 32, 672
BseNI ACTGG 4 cut(s) 84, 163, 599, 819
BseRI GAGGAG 1 cut(s) 620
BsgI GTGCAG 1 cut(s) 543
Bsh1236I CGCG 1 cut(s) 459
BshFI GGCC 2 cut(s) 307, 892
BsiHKCI CYCGRG 1 cut(s) 404
BsiSI CCGG 1 cut(s) 1010
BslFI GGGAC 1 cut(s) 825
BslI CCNNNNNNNGG 4 cut(s) 385, 670, 875, 955
BsmAI GTCTC 3 cut(s) 352, 407, 853
BsmFI GGGAC 1 cut(s) 825
BsnI GGCC 2 cut(s) 307, 892
BsoBI CYCGRG 1 cut(s) 404
Bsp1407I TGTACA 1 cut(s) 471
Bsp143I GATC 3 cut(s) 214, 623, 967
Bsp19I CCATGG 1 cut(s) 301
BspACI CCGC 2 cut(s) 459, 851
BspANI GGCC 2 cut(s) 307, 892
BspCNI CTCAG 2 cut(s) 33, 673
BspFNI CGCG 1 cut(s) 459
BspPI GGATC 1 cut(s) 962
BsrDI GCAATG 2 cut(s) 237, 280
BsrGI TGTACA 1 cut(s) 471
BsrI ACTGG 4 cut(s) 84, 163, 599, 819
BssECI CCNNGG 1 cut(s) 301
BssMI GATC 3 cut(s) 214, 623, 967
BssT1I CCWWGG 1 cut(s) 301
Bst4CI ACNGT 3 cut(s) 317, 422, 794
BstAUI TGTACA 1 cut(s) 471
BstDEI CTNAG 6 cut(s) 41, 211, 218, 494, 681, 987
BstDSI CCRYGG 1 cut(s) 301
BstF5I GGATG 2 cut(s) 603, 940
BstFNI CGCG 1 cut(s) 459
BstHHI GCGC 1 cut(s) 230
BstKTI GATC 3 cut(s) 217, 626, 970
BstMAI GTCTC 3 cut(s) 352, 407, 853
BstMBI GATC 3 cut(s) 214, 623, 967
BstNSI RCATGY 2 cut(s) 578, 785
BstSCI CCNGG 1 cut(s) 1009
BstUI CGCG 1 cut(s) 459
BstX2I RGATCY 2 cut(s) 214, 623
BstYI RGATCY 2 cut(s) 214, 623
BsuI GTATCC 1 cut(s) 725
BsuRI GGCC 2 cut(s) 307, 892
BtgI CCRYGG 1 cut(s) 301
BtsCI GGATG 2 cut(s) 603, 940
BtsIMutI CAGTG 1 cut(s) 33
CfoI GCGC 1 cut(s) 230
Cfr13I GGNCC 3 cut(s) 305, 540, 1039
Csp6I GTAC 3 cut(s) 455, 472, 592
CviAII CATG 9 cut(s) 119, 302, 322, 507, 575, 782, 822, 888, 1033
CviJI RGCY 6 cut(s) 307, 892, 900, 931, 986, 1023
CviKI_1 RGCY 6 cut(s) 307, 892, 900, 931, 986, 1023
CviQI GTAC 3 cut(s) 455, 472, 592
DdeI CTNAG 6 cut(s) 41, 211, 218, 494, 681, 987
DpnI GATC 3 cut(s) 216, 625, 969
DpnII GATC 3 cut(s) 214, 623, 967
EaeI YGGCCR 1 cut(s) 890
Eco130I CCWWGG 1 cut(s) 301
Eco47I GGWCC 2 cut(s) 540, 1039
Eco88I CYCGRG 1 cut(s) 404
EcoRI GAATTC 1 cut(s) 502
EcoT14I CCWWGG 1 cut(s) 301
EcoT22I ATGCAT 1 cut(s) 237
ErhI CCWWGG 1 cut(s) 301
FaeI CATG 9 cut(s) 122, 305, 325, 510, 578, 785, 825, 891, 1036
FalI AAGNNNNNCTT 4 cut(s) 161, 193, 846, 878
FaqI GGGAC 1 cut(s) 825
FatI CATG 9 cut(s) 118, 301, 321, 506, 574, 781, 821, 887, 1032
FauI CCCGC 1 cut(s) 452
FauNDI CATATG 1 cut(s) 874
FblI GTMKAC 1 cut(s) 486
FokI GGATG 2 cut(s) 610, 947
FspBI CTAG 2 cut(s) 689, 999
FspI TGCGCA 1 cut(s) 229
GlaI GCGC 1 cut(s) 229
HaeIII GGCC 2 cut(s) 307, 892
HapII CCGG 1 cut(s) 1010
HhaI GCGC 1 cut(s) 230
Hin1II CATG 9 cut(s) 122, 305, 325, 510, 578, 785, 825, 891, 1036
Hin6I GCGC 1 cut(s) 228
HinP1I GCGC 1 cut(s) 228
HinfI GANTC 4 cut(s) 45, 334, 832, 841
HpaII CCGG 1 cut(s) 1010
HphI GGTGA 1 cut(s) 410
Hpy166II GTNNAC 3 cut(s) 487, 592, 1042
Hpy188I TCNGA 3 cut(s) 50, 426, 695
Hpy188III TCNNGA 3 cut(s) 110, 154, 355
Hpy8I GTNNAC 3 cut(s) 487, 592, 1042
HpyCH4III ACNGT 3 cut(s) 317, 422, 794
HpyCH4IV ACGT 1 cut(s) 880
HpyCH4V TGCA 9 cut(s) 60, 167, 185, 235, 273, 288, 524, 569, 620
HpyF3I CTNAG 6 cut(s) 41, 211, 218, 494, 681, 987
HpySE526I ACGT 1 cut(s) 880
Hsp92II CATG 9 cut(s) 122, 305, 325, 510, 578, 785, 825, 891, 1036
HspAI GCGC 1 cut(s) 228
Kzo9I GATC 3 cut(s) 214, 623, 967
LweI GCATC 2 cut(s) 244, 297
MaeI CTAG 2 cut(s) 689, 999
MaeII ACGT 1 cut(s) 880
MaeIII GTNAC 3 cut(s) 137, 171, 794
MalI GATC 3 cut(s) 216, 625, 969
MboI GATC 3 cut(s) 214, 623, 967
MboII GAAGA 3 cut(s) 460, 770, 836
MflI RGATCY 2 cut(s) 214, 623
MlsI TGGCCA 1 cut(s) 892
MluCI AATT 4 cut(s) 78, 91, 465, 502
MluNI TGGCCA 1 cut(s) 892
MmeI TCCRAC 1 cut(s) 673
MnlI CCTC 7 cut(s) 23, 475, 598, 649, 689, 757, 1030
Mox20I TGGCCA 1 cut(s) 892
Mph1103I ATGCAT 1 cut(s) 237
MscI TGGCCA 1 cut(s) 892
MseI TTAA 3 cut(s) 77, 393, 1005
MslI CAYNNNNRTG 2 cut(s) 579, 1031
Msp20I TGGCCA 1 cut(s) 892
MspI CCGG 1 cut(s) 1010
MspR9I CCNGG 1 cut(s) 1011
MvnI CGCG 1 cut(s) 459
NciI CCSGG 1 cut(s) 1011
NcoI CCATGG 1 cut(s) 301
NdeI CATATG 1 cut(s) 874
NdeII GATC 3 cut(s) 214, 623, 967
NlaIII CATG 9 cut(s) 122, 305, 325, 510, 578, 785, 825, 891, 1036
NmuCI GTSAC 3 cut(s) 137, 171, 794
NsbI TGCGCA 1 cut(s) 229
NsiI ATGCAT 1 cut(s) 237
NspI RCATGY 2 cut(s) 578, 785
PciI ACATGT 1 cut(s) 574
PfeI GAWTC 4 cut(s) 45, 334, 832, 841
PflMI CCANNNNNTGG 4 cut(s) 385, 670, 875, 955
PscI ACATGT 1 cut(s) 574
PshBI ATTAAT 2 cut(s) 77, 393
PspPI GGNCC 3 cut(s) 305, 540, 1039
PsuI RGATCY 2 cut(s) 214, 623
RsaI GTAC 3 cut(s) 456, 473, 593
RsaNI GTAC 3 cut(s) 455, 472, 592
RseI CAYNNNNRTG 2 cut(s) 579, 1031
SaqAI TTAA 3 cut(s) 77, 393, 1005
Sau3AI GATC 3 cut(s) 214, 623, 967
Sau96I GGNCC 3 cut(s) 305, 540, 1039
ScrFI CCNGG 1 cut(s) 1011
SetI ASST 8 cut(s) 373, 486, 558, 660, 883, 902, 988, 1041
SfaNI GCATC 2 cut(s) 244, 297
SinI GGWCC 2 cut(s) 540, 1039
SmiMI CAYNNNNRTG 2 cut(s) 579, 1031
SmlI CTYRAG 1 cut(s) 751
SmoI CTYRAG 1 cut(s) 751
SpeI ACTAGT 1 cut(s) 688
Sse9I AATT 4 cut(s) 78, 91, 465, 502
SsiI CCGC 2 cut(s) 459, 851
SspMI CTAG 2 cut(s) 689, 999
StyD4I CCNGG 1 cut(s) 1009
StyI CCWWGG 1 cut(s) 301
TaaI ACNGT 3 cut(s) 317, 422, 794
TaiI ACGT 1 cut(s) 883
TaqI TCGA 2 cut(s) 341, 1071
TasI AATT 4 cut(s) 78, 91, 465, 502
TatI WGTACW 2 cut(s) 471, 591
TfiI GAWTC 4 cut(s) 45, 334, 832, 841
Tru1I TTAA 3 cut(s) 77, 393, 1005
Tru9I TTAA 3 cut(s) 77, 393, 1005
TscAI CASTG 1 cut(s) 33
TseFI GTSAC 3 cut(s) 137, 171, 794
Tsp45I GTSAC 3 cut(s) 137, 171, 794
TspDTI ATGAA 4 cut(s) 85, 495, 619, 951
TspRI CASTG 1 cut(s) 33
Van91I CCANNNNNTGG 4 cut(s) 385, 670, 875, 955
VpaK11BI GGWCC 2 cut(s) 540, 1039
VspI ATTAAT 2 cut(s) 77, 393
XapI RAATTY 2 cut(s) 91, 502
XceI RCATGY 2 cut(s) 578, 785
XmiI GTMKAC 1 cut(s) 486
XspI CTAG 2 cut(s) 689, 999
Zsp2I ATGCAT 1 cut(s) 237
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.