Rh2AG441300

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000234
Physical Location & Seq
Reverse (-)
65226715 .. 65235377
8663 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG441300.1

Sequence Viewer

Length: 873 bp
ATGGTGGGAGGAGTTGACGCCAAAATCGATCTTTCTCTCACTCTGTCATGGATTTCTGGGTTTTCACTGGTCTTGCTCTTGGAGTCTTGGTTTGATTCAGAAATCTTCAAGCTCGGAGAAAAATTGAGAATATTTGGTTCAAGCAATGGCCTTGTGTGTATCTCTGATGAAAAACTTCATCCAAATAGTCGTATATGCATATGGAACCCATCTATTCGGAAGTCTAGGACTCTTCCGAATATTGAGACGCATTTGTCTGATATAAGTACTACCCATCTCTCATTTGGATTCCATCCAGAGCTTGATGATTACATAGTTGTGAGGATTGTCCATCCTTATATAGGGAGTCCTGGAGTTGAGGTTTACACCCTTGGTACAAACTCTTGGAAGAGCATTCGGGGAACAGCATATTGGACTGCTAAGACAGGTTCAGAATATAAGATTGTATCATTTGATGCGGGAAGTGAAGAATTTCAAGAACTGATCGTGCCAAAGAAAAATTCCTTTACGGCGCTGCAAGTGTGCGAGGAATCACTTTGCCTCTTTCAATACGAGTGGGAAAGGCTGGAATGGTCAGGAGAATGGACCATTGACTCATGGATGATGAAAGAGCGGTCTTTGAACAAGGTGGATTCAAGTTCACTCGGGAAAAGCGGATGCTATTTTCCACTCAGCTCTAGCATAGATAATAAACTACTCATTGCATATATGGATATAGATATTAGAGATGAATATCGAGTAGTGGCTGCCGAAGTGGCTTTATATAACATTGAATCCAAACAATTAAATAGAACTGGAATTAGGTTGAAGCGGCTACGTGGCGGCTTTGATGGCGGTGCTTACATTGAAAGTCTGGTTTTGCTCGCTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

290

Amino Acids

32.81

Weight (kDa)

5.28

Isoelectric Point (pI)

35.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 41 - 144 3.9e-06 KIB1-4 beta-propeller
FBA_3 PF08268 43 - 184 2.6e-12 F-box associated beta propeller domain
FBA_1 PF07734 47 - 209 5.3e-10 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000234)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18880 FvH4_2g02020 FvH4_2g02020 FvH4_2g37110 FvH4_6g33750 FvH4_6g33770 FvH4_6g34412 FvH4_7g08973
malus_domestica MD09G1191300.v1.1 MD09G1281000.v1.1 MD09G1281100.v1.1 MD09G1281200.v1.1 MD12G1050800.v1.1
prunus_persica Prupe.1G188300_v2.0.a1 Prupe.2G120700_v2.0.a1 Prupe.3G013300_v2.0.a1 Prupe.3G013500_v2.0.a1 Prupe.3G047100_v2.0.a1 Prupe.3G047200_v2.0.a1 Prupe.3G047300_v2.0.a1 Prupe.3G047400_v2.0.a1 Prupe.6G319100_v2.0.a1
pyrus_communis pycom09g18870 pycom11g17570 pycom12g04520 pycom14g04100
rosa_chinensis RchiOBHm_Chr1g0321721 RchiOBHm_Chr1g0321751 RchiOBHm_Chr1g0344471 RchiOBHm_Chr1g0344741 RchiOBHm_Chr1g0344891 RchiOBHm_Chr2g0143631 RchiOBHm_Chr2g0143641 RchiOBHm_Chr2g0144781 RchiOBHm_Chr3g0458821 RchiOBHm_Chr6g0245251 RchiOBHm_Chr6g0304681
rosa_laevigata RLG00000007681 RLG00000010222 RLG00000013320 RLG00000015313 RLG00000017431 RLG00000017439 RLG00000020055 RLG00000020056 RLG00000020112 RLG00000025133 RLG00000028872 RLG00000028881 RLG00000028884 RLG00000030407
rosa_multiflora Rmu_co8324891.1_g000001 Rmu_sc0000087.1_g000002 Rmu_sc0001288.1_g000009 Rmu_sc0001634.1_g000031 Rmu_sc0001942.1_g000032 Rmu_sc0003410.1_g000036 Rmu_sc0003909.1_g000002 Rmu_sc0004340.1_g000024 Rmu_sc0005308.1_g000008 Rmu_sc0005705.1_g000040 Rmu_sc0016560.1_g000002 Rmu_ssc0000167.1_g000005
rosa_roxburghii Rroxscaffold_2G00101580 Rroxscaffold_4G00309600 Rroxscaffold_4G00309710 Rroxscaffold_4G00327490 Rroxscaffold_5G00333360 Rroxscaffold_6G00420770 Rroxscaffold_7G00163600 Rroxscaffold_7G00215330
rosa_rugosa Rorug01G0174400 Rorug02G0213500 Rorug02G0384500 Rorug02G0384500 Rorug02G0384500 Rorug03G0032100 Rorug03G0301100 Rorug05G0513100
rosa_samantha Rh1AG045800 Rh1AG046600 Rh1AG191000 Rh1AG191200 Rh1AG192400 Rh1BG045100 Rh1BG157400 Rh1BG158200 Rh1CG048800 Rh1CG176300 Rh1CG177700 Rh1DG053100 Rh1DG055000 Rh1DG188800 Rh2AG180100 Rh2AG180200 Rh2AG436400 Rh2AG436500 Rh2AG441300 Rh2BG444000 Rh2BG444100 Rh2BG452000 Rh2CG422700 Rh2CG422800 Rh2CG428600 Rh2DG185900 Rh2DG186000 Rh2DG454300 Rh2DG454400 Rh2DG461700 Rh3AG089700 Rh3BG092600 Rh3CG092900 Rh3DG093600 Rh4AG005900 Rh4DG004900 Rh4DG010300 Rh4DG010400 Rh4DG230700 Rh6AG028300 Rh6AG445500 Rh6BG024500 Rh6BG456400 Rh6BG456500 Rh6CG022600 Rh6CG458300 Rh6CG458400 Rh6DG022600 Rh6DG445500
rosa_wichuraiana Rw1G004020 Rw1G004060 Rw1G004180 Rw1G015690 Rw1G015790 Rw2G014090 Rw2G035570 Rw2G035580 Rw2G036090 Rw3G007680 Rw6G002380 Rw6G038740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 253
AccBSI CCGCTC 1 cut(s) 613
AciI CCGC 6 cut(s) 458, 613, 654, 811, 822, 834
AcsI RAATTY 2 cut(s) 470, 499
AcyI GRCGYC 1 cut(s) 18
AfaI GTAC 2 cut(s) 268, 376
AfiI CCNNNNNNNGG 1 cut(s) 341
AgsI TTSAA 9 cut(s) 109, 141, 476, 548, 622, 636, 773, 808, 848
AjnI CCWGG 1 cut(s) 349
AjuI GAANNNNNNNTTGG 4 cut(s) 394, 426, 484, 516
AluBI AGCT 3 cut(s) 112, 301, 675
AluI AGCT 3 cut(s) 112, 301, 675
Alw26I GTCTC 1 cut(s) 239
Ama87I CYCGRG 1 cut(s) 644
AoxI GGCC 1 cut(s) 148
ApeKI GCWGC 2 cut(s) 514, 746
ApoI RAATTY 2 cut(s) 470, 499
Asp700I GAANNNNTTC 2 cut(s) 174, 471
AspLEI GCGC 1 cut(s) 514
AspS9I GGNCC 1 cut(s) 585
AvaI CYCGRG 1 cut(s) 644
AvaII GGWCC 1 cut(s) 585
BbvI GCAGC 2 cut(s) 501, 733
BccI CCATC 5 cut(s) 217, 282, 300, 339, 824
BceAI ACGGC 1 cut(s) 525
BciT130I CCWGG 1 cut(s) 351
BcoDI GTCTC 1 cut(s) 239
BfaI CTAG 2 cut(s) 225, 678
BfoI RGCGCY 1 cut(s) 515
BglI GCCNNNNNGGC 1 cut(s) 755
BisI GCNGC 4 cut(s) 515, 747, 812, 823
BlsI GCNGC 4 cut(s) 516, 748, 813, 824
BmcAI AGTACT 1 cut(s) 268
Bme1390I CCNGG 1 cut(s) 351
Bme18I GGWCC 1 cut(s) 585
BmeT110I CYCGRG 1 cut(s) 644
BmgT120I GGNCC 1 cut(s) 585
BmiI GGNNCC 1 cut(s) 206
BmrFI CCNGG 1 cut(s) 351
BmsI GCATC 2 cut(s) 445, 647
BpmI CTGGAG 1 cut(s) 372
Bsa29I ATCGAT 1 cut(s) 27
BsaAI YACGTR 1 cut(s) 818
BsaBI GATNNNNATC 1 cut(s) 732
BsaHI GRCGYC 1 cut(s) 18
BsaJI CCNNGG 1 cut(s) 370
BsaXI ACNNNNNCTCC 4 cut(s) 74, 104, 345, 375
Bsc4I CCNNNNNNNGG 1 cut(s) 341
Bse1I ACTGG 2 cut(s) 72, 799
Bse3DI GCAATG 2 cut(s) 151, 699
Bse8I GATNNNNATC 1 cut(s) 732
BseBI CCWGG 1 cut(s) 351
BseCI ATCGAT 1 cut(s) 27
BseDI CCNNGG 1 cut(s) 370
BseGI GGATG 5 cut(s) 178, 292, 331, 606, 662
BseJI GATNNNNATC 1 cut(s) 732
BseLI CCNNNNNNNGG 1 cut(s) 341
BseMI GCAATG 2 cut(s) 151, 699
BseMII CTCAG 1 cut(s) 685
BseNI ACTGG 2 cut(s) 72, 799
BseRI GAGGAG 1 cut(s) 24
BseXI GCAGC 2 cut(s) 501, 733
BshFI GGCC 1 cut(s) 150
BshVI ATCGAT 1 cut(s) 27
BsiHKCI CYCGRG 1 cut(s) 644
BslI CCNNNNNNNGG 1 cut(s) 341
BsmAI GTCTC 1 cut(s) 239
BsmBI CGTCTC 1 cut(s) 239
BsmI GAATGC 1 cut(s) 393
BsnI GGCC 1 cut(s) 150
BsoBI CYCGRG 1 cut(s) 644
Bsp143I GATC 2 cut(s) 28, 483
BspACI CCGC 6 cut(s) 458, 613, 654, 811, 822, 834
BspANI GGCC 1 cut(s) 150
BspCNI CTCAG 1 cut(s) 684
BspDI ATCGAT 1 cut(s) 27
BspLI GGNNCC 1 cut(s) 206
BspQI GCTCTTC 1 cut(s) 383
BsrBI CCGCTC 1 cut(s) 613
BsrDI GCAATG 2 cut(s) 151, 699
BsrI ACTGG 2 cut(s) 72, 799
BssECI CCNNGG 1 cut(s) 370
BssMI GATC 2 cut(s) 28, 483
BssNI GRCGYC 1 cut(s) 18
BssT1I CCWWGG 1 cut(s) 370
Bst2UI CCWGG 1 cut(s) 351
Bst6I CTCTTC 2 cut(s) 237, 383
BstACI GRCGYC 1 cut(s) 18
BstBAI YACGTR 1 cut(s) 818
BstC8I GCNNGC 1 cut(s) 864
BstDEI CTNAG 2 cut(s) 420, 671
BstENI CCTNNNNNAGG 1 cut(s) 339
BstF5I GGATG 5 cut(s) 178, 292, 331, 606, 662
BstH2I RGCGCY 1 cut(s) 515
BstHHI GCGC 1 cut(s) 514
BstKTI GATC 2 cut(s) 31, 486
BstMAI GTCTC 1 cut(s) 239
BstMBI GATC 2 cut(s) 28, 483
BstMWI GCNNNNNNNGC 2 cut(s) 755, 831
BstNI CCWGG 1 cut(s) 351
BstSCI CCNGG 1 cut(s) 349
BstV1I GCAGC 2 cut(s) 501, 733
Bsu15I ATCGAT 1 cut(s) 27
BsuRI GGCC 1 cut(s) 150
BsuTUI ATCGAT 1 cut(s) 27
BtsCI GGATG 5 cut(s) 178, 292, 331, 606, 662
BtsIMutI CAGTG 1 cut(s) 65
Cac8I GCNNGC 1 cut(s) 864
CfoI GCGC 1 cut(s) 514
Cfr13I GGNCC 1 cut(s) 585
ClaI ATCGAT 1 cut(s) 27
CseI GACGC 2 cut(s) 26, 256
Csp6I GTAC 2 cut(s) 267, 375
CspCI CAANNNNNGTGG 2 cut(s) 536, 571
CviAII CATG 2 cut(s) 48, 597
CviJI RGCY 9 cut(s) 112, 150, 301, 565, 675, 746, 758, 814, 825
CviKI_1 RGCY 9 cut(s) 112, 150, 301, 565, 675, 746, 758, 814, 825
CviQI GTAC 2 cut(s) 267, 375
DdeI CTNAG 2 cut(s) 420, 671
DpnI GATC 2 cut(s) 30, 485
DpnII GATC 2 cut(s) 28, 483
DrdI GACNNNNNNGTC 1 cut(s) 253
DseDI GACNNNNNNGTC 1 cut(s) 253
Eam1104I CTCTTC 2 cut(s) 237, 383
EarI CTCTTC 2 cut(s) 237, 383
Eco130I CCWWGG 1 cut(s) 370
Eco47I GGWCC 1 cut(s) 585
Eco88I CYCGRG 1 cut(s) 644
EcoNI CCTNNNNNAGG 1 cut(s) 339
EcoRII CCWGG 1 cut(s) 349
EcoT14I CCWWGG 1 cut(s) 370
EcoT22I ATGCAT 1 cut(s) 200
ErhI CCWWGG 1 cut(s) 370
Esp3I CGTCTC 1 cut(s) 239
FaeI CATG 2 cut(s) 51, 600
FatI CATG 2 cut(s) 47, 596
FauI CCCGC 1 cut(s) 451
FauNDI CATATG 1 cut(s) 200
Fnu4HI GCNGC 4 cut(s) 515, 747, 812, 823
FokI GGATG 5 cut(s) 165, 279, 318, 613, 669
Fsp4HI GCNGC 4 cut(s) 515, 747, 812, 823
FspBI CTAG 2 cut(s) 225, 678
GlaI GCGC 1 cut(s) 513
GluI GCNGC 4 cut(s) 515, 747, 812, 823
GsuI CTGGAG 1 cut(s) 372
HaeII RGCGCY 1 cut(s) 515
HaeIII GGCC 1 cut(s) 150
HgaI GACGC 2 cut(s) 26, 256
HhaI GCGC 1 cut(s) 514
Hin1I GRCGYC 1 cut(s) 18
Hin1II CATG 2 cut(s) 51, 600
Hin6I GCGC 1 cut(s) 512
HinP1I GCGC 1 cut(s) 512
HincII GTYRAC 1 cut(s) 16
HindII GTYRAC 1 cut(s) 16
HinfI GANTC 9 cut(s) 83, 95, 229, 288, 346, 530, 593, 632, 773
Hpy166II GTNNAC 3 cut(s) 16, 364, 641
Hpy188I TCNGA 7 cut(s) 100, 116, 166, 219, 237, 259, 433
Hpy188III TCNNGA 4 cut(s) 296, 476, 576, 646
Hpy8I GTNNAC 3 cut(s) 16, 364, 641
HpyCH4IV ACGT 1 cut(s) 817
HpyCH4V TGCA 3 cut(s) 198, 517, 704
HpyF10VI GCNNNNNNNGC 2 cut(s) 755, 831
HpyF3I CTNAG 2 cut(s) 420, 671
HpySE526I ACGT 1 cut(s) 817
Hsp92I GRCGYC 1 cut(s) 18
Hsp92II CATG 2 cut(s) 51, 600
HspAI GCGC 1 cut(s) 512
Kzo9I GATC 2 cut(s) 28, 483
LguI GCTCTTC 1 cut(s) 383
Lsp1109I GCAGC 2 cut(s) 501, 733
LweI GCATC 2 cut(s) 445, 647
MaeI CTAG 2 cut(s) 225, 678
MaeII ACGT 1 cut(s) 817
MalI GATC 2 cut(s) 30, 485
MbiI CCGCTC 1 cut(s) 613
MboI GATC 2 cut(s) 28, 483
MboII GAAGA 4 cut(s) 97, 224, 400, 479
MluCI AATT 5 cut(s) 122, 470, 499, 782, 798
MlyI GAGTC 4 cut(s) 92, 223, 355, 587
MnlI CCTC 4 cut(s) 315, 352, 520, 551
Mph1103I ATGCAT 1 cut(s) 200
MroXI GAANNNNTTC 2 cut(s) 174, 471
MseI TTAA 2 cut(s) 785, 871
MslI CAYNNNNRTG 1 cut(s) 317
MspR9I CCNGG 1 cut(s) 351
Mva1269I GAATGC 1 cut(s) 393
MvaI CCWGG 1 cut(s) 351
MwoI GCNNNNNNNGC 2 cut(s) 755, 831
NdeI CATATG 1 cut(s) 200
NdeII GATC 2 cut(s) 28, 483
NlaIII CATG 2 cut(s) 51, 600
NlaIV GGNNCC 1 cut(s) 206
NsiI ATGCAT 1 cut(s) 200
PciSI GCTCTTC 1 cut(s) 383
PcsI WCGNNNNNNNCGW 1 cut(s) 24
PctI GAATGC 1 cut(s) 393
PdmI GAANNNNTTC 2 cut(s) 174, 471
PfeI GAWTC 5 cut(s) 95, 288, 530, 632, 773
PfoI TCCNGGA 1 cut(s) 349
PkrI GCNGC 4 cut(s) 516, 748, 813, 824
PleI GAGTC 4 cut(s) 91, 223, 354, 587
PpsI GAGTC 4 cut(s) 91, 223, 354, 587
Ppu21I YACGTR 1 cut(s) 818
Psp6I CCWGG 1 cut(s) 349
PspGI CCWGG 1 cut(s) 349
PspN4I GGNNCC 1 cut(s) 206
PspPI GGNCC 1 cut(s) 585
RsaI GTAC 2 cut(s) 268, 376
RsaNI GTAC 2 cut(s) 267, 375
RseI CAYNNNNRTG 1 cut(s) 317
SapI GCTCTTC 1 cut(s) 383
SaqAI TTAA 2 cut(s) 785, 871
SatI GCNGC 4 cut(s) 515, 747, 812, 823
Sau3AI GATC 2 cut(s) 28, 483
Sau96I GGNCC 1 cut(s) 585
ScaI AGTACT 1 cut(s) 268
SchI GAGTC 4 cut(s) 92, 223, 355, 587
ScrFI CCNGG 1 cut(s) 351
SetI ASST 8 cut(s) 114, 303, 363, 430, 630, 677, 806, 820
SfaNI GCATC 2 cut(s) 445, 647
SinI GGWCC 1 cut(s) 585
SmiMI CAYNNNNRTG 1 cut(s) 317
Sse9I AATT 5 cut(s) 122, 470, 499, 782, 798
SsiI CCGC 6 cut(s) 458, 613, 654, 811, 822, 834
SspI AATATT 2 cut(s) 132, 241
SspMI CTAG 2 cut(s) 225, 678
StyD4I CCNGG 1 cut(s) 349
StyI CCWWGG 1 cut(s) 370
TaiI ACGT 1 cut(s) 820
TaqI TCGA 2 cut(s) 27, 736
TasI AATT 5 cut(s) 122, 470, 499, 782, 798
TatI WGTACW 1 cut(s) 266
TauI GCSGC 2 cut(s) 814, 825
TfiI GAWTC 5 cut(s) 95, 288, 530, 632, 773
Tru1I TTAA 2 cut(s) 785, 871
Tru9I TTAA 2 cut(s) 785, 871
TscAI CASTG 1 cut(s) 72
TseI GCWGC 2 cut(s) 514, 746
TspDTI ATGAA 4 cut(s) 167, 183, 620, 744
TspRI CASTG 1 cut(s) 72
VpaK11BI GGWCC 1 cut(s) 585
XagI CCTNNNNNAGG 1 cut(s) 339
XapI RAATTY 2 cut(s) 470, 499
XcmI CCANNNNNNNNNTGG 1 cut(s) 281
XmnI GAANNNNTTC 2 cut(s) 174, 471
XspI CTAG 2 cut(s) 225, 678
ZrmI AGTACT 1 cut(s) 268
Zsp2I ATGCAT 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.