pycom14g04100

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0000234
Physical Location & Seq
Forward (+)
3511722 .. 3512717
996 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g04100.1

Sequence Viewer

Length: 996 bp
ATGTTTAACCACACTATACCCGAAGAAATCTTGTTTGATATCCTTGCAACATTGCCGGTGAAATCTCTTTTGCGGTTTAGATGTGTTTGTAAATCTTGGAAATTTTTAATTAGCAGTTCTATTTTCAAAAATGCCCATCTCAAAAAAAACGTCGCGGAAAATTCTTGTGAGTATCTACTCATCCAGACAGACAAAAAAAATTGTTTGTCACTGTTTGATGCTGAAACATTGTCCAAGTGTTTGGATATAGAGCTTCATGCGAACAAATTAGGTTTCAGATTCTTTGTGCATGGTTCGTGCAATGGATTGGTTTGTATCTCGGGTTGTAATTTCTTTAGTTTGAATAGTCCTATATACTTGTGGAATCCATCAATCAGAAAAATCAAGAGGCTTCCTAATGGCTGCATTCGGCAAATAATAAATTTCGTTAATCTTGGTTTCGGGTTTCACAGTGGCGAAAACGACTATAAGGTTGTGAGGCTTGTTGGGGCTTCGCTTCCACGAGAGAATGTCGTCCTTGAAGTTGAGGTTTACAGTCTTAGATCAAATGCTTGGAGAAGAATAACTACGATGCCTTCTTTTTCAGATAATGCCGTTTTTTGGGAATCCAGATGTACCTTCTTGGATGGAGTTGTGTATTGGATTATAAAAGAGCATTCTTGCACTTCAATCCTTGCTTTCGATTTGGGCAGTGAGGTTTCACGAAAGATTATGTTTCCTGAAATAGTACAAAATCAAATGCGTATTGGAGTGTTTGAAAAATCACTCGCTTTGTTCCATACAAGAACTGGTCTTGCTGTGATGGATTCGTATGTTGACATATGGGTTCTGGAAGTGGATGTTTGGAAAATGATCCGGACGATTTATTTGCCTAGTAGTACTGGATTTGTAGCCTGGCCATTGGGAATTAGAACAAATCTTGGCAAAGTTCACATGATTCAGGATAGTTGGGACCCTAAGTTTGTTTTATATCGATCCAGAGTCACACCAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1

Protein Analysis

332

Amino Acids

38.03

Weight (kDa)

9.0

Isoelectric Point (pI)

36.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 6 - 43 8.3e-11 F-box domain
F-box-like PF12937 6 - 43 7.7e-09 F-box-like
FBA_3 PF08268 77 - 282 7.9e-17 F-box associated beta propeller domain
b-prop_At3g26010-like PF24750 87 - 294 3.9e-07 F-box protein At3g26010-like, beta-propeller
FBA_1 PF07734 97 - 277 2.4e-18 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000234)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18880 FvH4_2g02020 FvH4_2g02020 FvH4_2g37110 FvH4_6g33750 FvH4_6g33770 FvH4_6g34412 FvH4_7g08973
malus_domestica MD09G1191300.v1.1 MD09G1281000.v1.1 MD09G1281100.v1.1 MD09G1281200.v1.1 MD12G1050800.v1.1
prunus_persica Prupe.1G188300_v2.0.a1 Prupe.2G120700_v2.0.a1 Prupe.3G013300_v2.0.a1 Prupe.3G013500_v2.0.a1 Prupe.3G047100_v2.0.a1 Prupe.3G047200_v2.0.a1 Prupe.3G047300_v2.0.a1 Prupe.3G047400_v2.0.a1 Prupe.6G319100_v2.0.a1
pyrus_communis pycom09g18870 pycom11g17570 pycom12g04520 pycom14g04100
rosa_chinensis RchiOBHm_Chr1g0321721 RchiOBHm_Chr1g0321751 RchiOBHm_Chr1g0344471 RchiOBHm_Chr1g0344741 RchiOBHm_Chr1g0344891 RchiOBHm_Chr2g0143631 RchiOBHm_Chr2g0143641 RchiOBHm_Chr2g0144781 RchiOBHm_Chr3g0458821 RchiOBHm_Chr6g0245251 RchiOBHm_Chr6g0304681
rosa_laevigata RLG00000007681 RLG00000010222 RLG00000013320 RLG00000015313 RLG00000017431 RLG00000017439 RLG00000020055 RLG00000020056 RLG00000020112 RLG00000025133 RLG00000028872 RLG00000028881 RLG00000028884 RLG00000030407
rosa_multiflora Rmu_co8324891.1_g000001 Rmu_sc0000087.1_g000002 Rmu_sc0001288.1_g000009 Rmu_sc0001634.1_g000031 Rmu_sc0001942.1_g000032 Rmu_sc0003410.1_g000036 Rmu_sc0003909.1_g000002 Rmu_sc0004340.1_g000024 Rmu_sc0005308.1_g000008 Rmu_sc0005705.1_g000040 Rmu_sc0016560.1_g000002 Rmu_ssc0000167.1_g000005
rosa_roxburghii Rroxscaffold_2G00101580 Rroxscaffold_4G00309600 Rroxscaffold_4G00309710 Rroxscaffold_4G00327490 Rroxscaffold_5G00333360 Rroxscaffold_6G00420770 Rroxscaffold_7G00163600 Rroxscaffold_7G00215330
rosa_rugosa Rorug01G0174400 Rorug02G0213500 Rorug02G0384500 Rorug02G0384500 Rorug02G0384500 Rorug03G0032100 Rorug03G0301100 Rorug05G0513100
rosa_samantha Rh1AG045800 Rh1AG046600 Rh1AG191000 Rh1AG191200 Rh1AG192400 Rh1BG045100 Rh1BG157400 Rh1BG158200 Rh1CG048800 Rh1CG176300 Rh1CG177700 Rh1DG053100 Rh1DG055000 Rh1DG188800 Rh2AG180100 Rh2AG180200 Rh2AG436400 Rh2AG436500 Rh2AG441300 Rh2BG444000 Rh2BG444100 Rh2BG452000 Rh2CG422700 Rh2CG422800 Rh2CG428600 Rh2DG185900 Rh2DG186000 Rh2DG454300 Rh2DG454400 Rh2DG461700 Rh3AG089700 Rh3BG092600 Rh3CG092900 Rh3DG093600 Rh4AG005900 Rh4DG004900 Rh4DG010300 Rh4DG010400 Rh4DG230700 Rh6AG028300 Rh6AG445500 Rh6BG024500 Rh6BG456400 Rh6BG456500 Rh6CG022600 Rh6CG458300 Rh6CG458400 Rh6DG022600 Rh6DG445500
rosa_wichuraiana Rw1G004020 Rw1G004060 Rw1G004180 Rw1G015690 Rw1G015790 Rw2G014090 Rw2G035570 Rw2G035580 Rw2G036090 Rw3G007680 Rw6G002380 Rw6G038740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 647
AccII CGCG 1 cut(s) 155
AccIII TCCGGA 1 cut(s) 855
AciI CCGC 2 cut(s) 73, 155
AclWI GGATC 2 cut(s) 847, 969
AcoI YGGCCR 1 cut(s) 896
AcsI RAATTY 3 cut(s) 101, 160, 421
AfaI GTAC 3 cut(s) 616, 729, 880
AfiI CCNNNNNNNGG 1 cut(s) 600
AgsI TTSAA 5 cut(s) 127, 343, 521, 669, 758
AjnI CCWGG 1 cut(s) 893
AluBI AGCT 1 cut(s) 253
AluI AGCT 1 cut(s) 253
AlwI GGATC 2 cut(s) 847, 969
Ama87I CYCGRG 1 cut(s) 319
Aor13HI TCCGGA 1 cut(s) 855
AoxI GGCC 1 cut(s) 896
ApeKI GCWGC 1 cut(s) 402
ApoI RAATTY 3 cut(s) 101, 160, 421
ArsI GACNNNNNNTTYG 6 cut(s) 135, 167, 850, 882, 944, 976
AspS9I GGNCC 1 cut(s) 952
AsuHPI GGTGA 1 cut(s) 70
AvaI CYCGRG 1 cut(s) 319
AvaII GGWCC 1 cut(s) 952
BalI TGGCCA 1 cut(s) 898
BarI GAAGNNNNNNTAC 2 cut(s) 550, 582
BauI CACGAG 1 cut(s) 501
BbvI GCAGC 1 cut(s) 389
BccI CCATC 4 cut(s) 144, 376, 620, 796
BceAI ACGGC 1 cut(s) 578
BcgI CGANNNNNNTGC 2 cut(s) 850, 884
BciT130I CCWGG 1 cut(s) 895
BfaI CTAG 1 cut(s) 873
BisI GCNGC 1 cut(s) 403
BlsI GCNGC 1 cut(s) 404
BmcAI AGTACT 1 cut(s) 880
Bme1390I CCNGG 1 cut(s) 895
Bme18I GGWCC 1 cut(s) 952
BmeT110I CYCGRG 1 cut(s) 319
BmgT120I GGNCC 1 cut(s) 952
BmiI GGNNCC 2 cut(s) 953, 954
BmrFI CCNGG 1 cut(s) 895
BmsI GCATC 2 cut(s) 208, 561
Bsa29I ATCGAT 1 cut(s) 973
BsaWI WCCGGW 1 cut(s) 855
Bsc4I CCNNNNNNNGG 1 cut(s) 600
Bse118I RCCGGY 1 cut(s) 55
Bse1I ACTGG 2 cut(s) 793, 886
Bse3DI GCAATG 2 cut(s) 50, 307
BseAI TCCGGA 1 cut(s) 855
BseBI CCWGG 1 cut(s) 895
BseCI ATCGAT 1 cut(s) 973
BseGI GGATG 3 cut(s) 180, 631, 844
BseLI CCNNNNNNNGG 1 cut(s) 600
BseMI GCAATG 2 cut(s) 50, 307
BseNI ACTGG 2 cut(s) 793, 886
BseXI GCAGC 1 cut(s) 389
Bsh1236I CGCG 1 cut(s) 155
BshFI GGCC 1 cut(s) 898
BshVI ATCGAT 1 cut(s) 973
BsiHKCI CYCGRG 1 cut(s) 319
BsiSI CCGG 2 cut(s) 56, 856
BslFI GGGAC 1 cut(s) 965
BslI CCNNNNNNNGG 1 cut(s) 600
BsmFI GGGAC 1 cut(s) 965
BsmI GAATGC 2 cut(s) 405, 655
BsnI GGCC 1 cut(s) 898
BsoBI CYCGRG 1 cut(s) 319
Bsp13I TCCGGA 1 cut(s) 855
Bsp143I GATC 3 cut(s) 542, 852, 974
BspACI CCGC 2 cut(s) 73, 155
BspANI GGCC 1 cut(s) 898
BspDI ATCGAT 1 cut(s) 973
BspEI TCCGGA 1 cut(s) 855
BspFNI CGCG 1 cut(s) 155
BspLI GGNNCC 2 cut(s) 953, 954
BspPI GGATC 2 cut(s) 847, 969
BsrDI GCAATG 2 cut(s) 50, 307
BsrFI RCCGGY 1 cut(s) 55
BsrI ACTGG 2 cut(s) 793, 886
BssAI RCCGGY 1 cut(s) 55
BssMI GATC 3 cut(s) 542, 852, 974
BssSI CACGAG 1 cut(s) 501
Bst2BI CACGAG 1 cut(s) 501
Bst2UI CCWGG 1 cut(s) 895
Bst4CI ACNGT 3 cut(s) 213, 452, 536
BstDEI CTNAG 2 cut(s) 539, 957
BstF5I GGATG 3 cut(s) 180, 631, 844
BstFNI CGCG 1 cut(s) 155
BstKTI GATC 3 cut(s) 545, 855, 977
BstMBI GATC 3 cut(s) 542, 852, 974
BstNI CCWGG 1 cut(s) 895
BstSCI CCNGG 1 cut(s) 893
BstUI CGCG 1 cut(s) 155
BstV1I GCAGC 1 cut(s) 389
BstXI CCANNNNNNTGG 1 cut(s) 241
Bsu15I ATCGAT 1 cut(s) 973
BsuRI GGCC 1 cut(s) 898
BsuTUI ATCGAT 1 cut(s) 973
BtsCI GGATG 3 cut(s) 180, 631, 844
BtsI GCAGTG 1 cut(s) 697
BtsIMutI CAGTG 3 cut(s) 209, 457, 697
Cfr10I RCCGGY 1 cut(s) 55
Cfr13I GGNCC 1 cut(s) 952
ClaI ATCGAT 1 cut(s) 973
Csp6I GTAC 3 cut(s) 615, 728, 879
CviAII CATG 3 cut(s) 257, 290, 934
CviJI RGCY 7 cut(s) 253, 391, 402, 481, 491, 893, 898
CviKI_1 RGCY 7 cut(s) 253, 391, 402, 481, 491, 893, 898
CviQI GTAC 3 cut(s) 615, 728, 879
DdeI CTNAG 2 cut(s) 539, 957
DpnI GATC 3 cut(s) 544, 854, 976
DpnII GATC 3 cut(s) 542, 852, 974
EaeI YGGCCR 1 cut(s) 896
Eco32I GATATC 1 cut(s) 40
Eco47I GGWCC 1 cut(s) 952
Eco88I CYCGRG 1 cut(s) 319
EcoO109I RGGNCCY 1 cut(s) 952
EcoRII CCWGG 1 cut(s) 893
EcoRV GATATC 1 cut(s) 40
FaeI CATG 3 cut(s) 260, 293, 937
FaqI GGGAC 1 cut(s) 965
FatI CATG 3 cut(s) 256, 289, 933
FauNDI CATATG 1 cut(s) 821
Fnu4HI GCNGC 1 cut(s) 403
FokI GGATG 3 cut(s) 167, 638, 851
Fsp4HI GCNGC 1 cut(s) 403
FspBI CTAG 1 cut(s) 873
GluI GCNGC 1 cut(s) 403
HaeIII GGCC 1 cut(s) 898
HapII CCGG 2 cut(s) 56, 856
Hin1II CATG 3 cut(s) 260, 293, 937
HincII GTYRAC 1 cut(s) 817
HindII GTYRAC 1 cut(s) 817
HinfI GANTC 6 cut(s) 279, 364, 605, 806, 937, 981
HpaII CCGG 2 cut(s) 56, 856
HphI GGTGA 1 cut(s) 70
Hpy166II GTNNAC 3 cut(s) 532, 817, 931
Hpy188I TCNGA 3 cut(s) 278, 377, 586
Hpy188III TCNNGA 9 cut(s) 184, 385, 609, 702, 719, 830, 856, 941, 978
Hpy8I GTNNAC 3 cut(s) 532, 817, 931
Hpy99I CGWCG 1 cut(s) 155
HpyAV CCTTC 2 cut(s) 585, 628
HpyCH4III ACNGT 3 cut(s) 213, 452, 536
HpyCH4IV ACGT 1 cut(s) 150
HpyCH4V TGCA 5 cut(s) 47, 289, 300, 405, 663
HpyF3I CTNAG 2 cut(s) 539, 957
HpySE526I ACGT 1 cut(s) 150
Hsp92II CATG 3 cut(s) 260, 293, 937
KflI GGGWCCC 1 cut(s) 952
Kpn2I TCCGGA 1 cut(s) 855
Kzo9I GATC 3 cut(s) 542, 852, 974
Lsp1109I GCAGC 1 cut(s) 389
LweI GCATC 2 cut(s) 208, 561
MaeI CTAG 1 cut(s) 873
MaeII ACGT 1 cut(s) 150
MaeIII GTNAC 2 cut(s) 207, 982
MalI GATC 3 cut(s) 544, 854, 976
MboI GATC 3 cut(s) 542, 852, 974
MboII GAAGA 2 cut(s) 35, 570
MlsI TGGCCA 1 cut(s) 898
MluCI AATT 8 cut(s) 101, 108, 160, 199, 266, 328, 421, 906
MluNI TGGCCA 1 cut(s) 898
MlyI GAGTC 1 cut(s) 990
MnlI CCTC 4 cut(s) 381, 471, 520, 688
Mox20I TGGCCA 1 cut(s) 898
MroI TCCGGA 1 cut(s) 855
MscI TGGCCA 1 cut(s) 898
MseI TTAA 4 cut(s) 6, 107, 429, 994
Msp20I TGGCCA 1 cut(s) 898
MspI CCGG 2 cut(s) 56, 856
MspR9I CCNGG 1 cut(s) 895
Mva1269I GAATGC 2 cut(s) 405, 655
MvaI CCWGG 1 cut(s) 895
MvnI CGCG 1 cut(s) 155
NdeI CATATG 1 cut(s) 821
NdeII GATC 3 cut(s) 542, 852, 974
NlaIII CATG 3 cut(s) 260, 293, 937
NlaIV GGNNCC 2 cut(s) 953, 954
NmuCI GTSAC 2 cut(s) 207, 982
PctI GAATGC 2 cut(s) 405, 655
PfeI GAWTC 5 cut(s) 279, 364, 605, 806, 937
PkrI GCNGC 1 cut(s) 404
PleI GAGTC 1 cut(s) 989
PpsI GAGTC 1 cut(s) 989
PpuMI RGGWCCY 1 cut(s) 952
PsiI TTATAA 1 cut(s) 647
Psp5II RGGWCCY 1 cut(s) 952
Psp6I CCWGG 1 cut(s) 893
PspGI CCWGG 1 cut(s) 893
PspN4I GGNNCC 2 cut(s) 953, 954
PspPI GGNCC 1 cut(s) 952
PspPPI RGGWCCY 1 cut(s) 952
RsaI GTAC 3 cut(s) 616, 729, 880
RsaNI GTAC 3 cut(s) 615, 728, 879
SaqAI TTAA 4 cut(s) 6, 107, 429, 994
SatI GCNGC 1 cut(s) 403
Sau3AI GATC 3 cut(s) 542, 852, 974
Sau96I GGNCC 1 cut(s) 952
ScaI AGTACT 1 cut(s) 880
SchI GAGTC 1 cut(s) 990
ScrFI CCNGG 1 cut(s) 895
SetI ASST 7 cut(s) 153, 255, 274, 474, 531, 620, 699
SfaNI GCATC 2 cut(s) 208, 561
SinI GGWCC 1 cut(s) 952
Sse9I AATT 8 cut(s) 101, 108, 160, 199, 266, 328, 421, 906
SsiI CCGC 2 cut(s) 73, 155
SspMI CTAG 1 cut(s) 873
StyD4I CCNGG 1 cut(s) 893
TaaI ACNGT 3 cut(s) 213, 452, 536
TaiI ACGT 1 cut(s) 153
TaqI TCGA 2 cut(s) 681, 973
TasI AATT 8 cut(s) 101, 108, 160, 199, 266, 328, 421, 906
TatI WGTACW 2 cut(s) 727, 878
TfiI GAWTC 5 cut(s) 279, 364, 605, 806, 937
Tru1I TTAA 4 cut(s) 6, 107, 429, 994
Tru9I TTAA 4 cut(s) 6, 107, 429, 994
TscAI CASTG 3 cut(s) 216, 457, 697
TseFI GTSAC 2 cut(s) 207, 982
TseI GCWGC 1 cut(s) 402
Tsp45I GTSAC 2 cut(s) 207, 982
TspDTI ATGAA 1 cut(s) 245
TspRI CASTG 3 cut(s) 216, 457, 697
VpaK11BI GGWCC 1 cut(s) 952
XapI RAATTY 3 cut(s) 101, 160, 421
XcmI CCANNNNNNNNNTGG 1 cut(s) 785
XspI CTAG 1 cut(s) 873
ZrmI AGTACT 1 cut(s) 880
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.