RLG00000020112

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000234
Physical Location & Seq
Reverse (-)
61105982 .. 61106767
786 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020112

Sequence Viewer

Length: 786 bp
ATGAGTAATTTCAACTACATCCCTGAAGAAATTGTGATCGATATCCTACAGAGACTATCCTCAAAATCCCTTATTCGGTTTAGGTGTGTATGCAAGTCATGGCGAGATTTGATTGGGAGCTCAAGTTTTGCTCGGTCACATCTTAATAGAAATGTAGCGAGACGCTCCAATAGATATCTGTATGCTTACCACCAGTCCTCAATAGATGAACCAGAACATTGTCAGTACTGGCTTTTCTCTACTGAAACATTTGAGCCGTGCTTGAATTTAAAACATCCCTTATGGACCAGAGAAAAATTGAGAATATATGGTTCAAGCAATGGCCTTGTGTGTATCTCTGATGAAAAACTTCATCCAAATAGTCGTATATGCATATGGAACCCATCTATTCGGAAGTCTAGGACTCTTCCGAATATTGAGACGCATTTGTCTGATATAAGTACTACCCATCTCTCATTTGGATTCCATCCAAAGCTTAATGATTACATAGTTGTGAGGATTGTCCTTCCTTATATGGGGAGTCCTAAAGTTGAGGTTTATACCCTTAGCACAAACTCTTGGAAGAGCATTTGGGCAACCCCTCCTTGGTTAAACTTCAATCGCATTTACTCCACAGCTGCATTTTTGAAGGGAACAGCATATTGGACTGCTAAGACAGGTTCAGAATATAAGATTGTAGCATTTGATGCGGGAAGTGAAGAATTTCAAGAACTGATGGTGCCAAAGAAAAATTCCTTCACGGCGCTGCTAGTGTGCGAGGAATCACTTTGCCTCTTTCAATACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

262

Amino Acids

30.37

Weight (kDa)

8.95

Isoelectric Point (pI)

41.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 5 - 43 1.1e-09 F-box-like
F-box PF00646 7 - 43 2.8e-11 F-box domain
Beta-prop_KIB1-4 PF03478 90 - 242 1.4e-08 KIB1-4 beta-propeller
FBA_3 PF08268 99 - 246 1.4e-16 F-box associated beta propeller domain
FBA_1 PF07734 106 - 245 2.8e-17 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000234)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18880 FvH4_2g02020 FvH4_2g02020 FvH4_2g37110 FvH4_6g33750 FvH4_6g33770 FvH4_6g34412 FvH4_7g08973
malus_domestica MD09G1191300.v1.1 MD09G1281000.v1.1 MD09G1281100.v1.1 MD09G1281200.v1.1 MD12G1050800.v1.1
prunus_persica Prupe.1G188300_v2.0.a1 Prupe.2G120700_v2.0.a1 Prupe.3G013300_v2.0.a1 Prupe.3G013500_v2.0.a1 Prupe.3G047100_v2.0.a1 Prupe.3G047200_v2.0.a1 Prupe.3G047300_v2.0.a1 Prupe.3G047400_v2.0.a1 Prupe.6G319100_v2.0.a1
pyrus_communis pycom09g18870 pycom11g17570 pycom12g04520 pycom14g04100
rosa_chinensis RchiOBHm_Chr1g0321721 RchiOBHm_Chr1g0321751 RchiOBHm_Chr1g0344471 RchiOBHm_Chr1g0344741 RchiOBHm_Chr1g0344891 RchiOBHm_Chr2g0143631 RchiOBHm_Chr2g0143641 RchiOBHm_Chr2g0144781 RchiOBHm_Chr3g0458821 RchiOBHm_Chr6g0245251 RchiOBHm_Chr6g0304681
rosa_laevigata RLG00000007681 RLG00000010222 RLG00000013320 RLG00000015313 RLG00000017431 RLG00000017439 RLG00000020055 RLG00000020056 RLG00000020112 RLG00000025133 RLG00000028872 RLG00000028881 RLG00000028884 RLG00000030407
rosa_multiflora Rmu_co8324891.1_g000001 Rmu_sc0000087.1_g000002 Rmu_sc0001288.1_g000009 Rmu_sc0001634.1_g000031 Rmu_sc0001942.1_g000032 Rmu_sc0003410.1_g000036 Rmu_sc0003909.1_g000002 Rmu_sc0004340.1_g000024 Rmu_sc0005308.1_g000008 Rmu_sc0005705.1_g000040 Rmu_sc0016560.1_g000002 Rmu_ssc0000167.1_g000005
rosa_roxburghii Rroxscaffold_2G00101580 Rroxscaffold_4G00309600 Rroxscaffold_4G00309710 Rroxscaffold_4G00327490 Rroxscaffold_5G00333360 Rroxscaffold_6G00420770 Rroxscaffold_7G00163600 Rroxscaffold_7G00215330
rosa_rugosa Rorug01G0174400 Rorug02G0213500 Rorug02G0384500 Rorug02G0384500 Rorug02G0384500 Rorug03G0032100 Rorug03G0301100 Rorug05G0513100
rosa_samantha Rh1AG045800 Rh1AG046600 Rh1AG191000 Rh1AG191200 Rh1AG192400 Rh1BG045100 Rh1BG157400 Rh1BG158200 Rh1CG048800 Rh1CG176300 Rh1CG177700 Rh1DG053100 Rh1DG055000 Rh1DG188800 Rh2AG180100 Rh2AG180200 Rh2AG436400 Rh2AG436500 Rh2AG441300 Rh2BG444000 Rh2BG444100 Rh2BG452000 Rh2CG422700 Rh2CG422800 Rh2CG428600 Rh2DG185900 Rh2DG186000 Rh2DG454300 Rh2DG454400 Rh2DG461700 Rh3AG089700 Rh3BG092600 Rh3CG092900 Rh3DG093600 Rh4AG005900 Rh4DG004900 Rh4DG010300 Rh4DG010400 Rh4DG230700 Rh6AG028300 Rh6AG445500 Rh6BG024500 Rh6BG456400 Rh6BG456500 Rh6CG022600 Rh6CG458300 Rh6CG458400 Rh6DG022600 Rh6DG445500
rosa_wichuraiana Rw1G004020 Rw1G004060 Rw1G004180 Rw1G015690 Rw1G015790 Rw2G014090 Rw2G035570 Rw2G035580 Rw2G036090 Rw3G007680 Rw6G002380 Rw6G038740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 427
AccB1I GGYRCC 1 cut(s) 718
AciI CCGC 1 cut(s) 689
AcsI RAATTY 3 cut(s) 265, 701, 730
AcuI CTGAAG 1 cut(s) 45
AfaI GTAC 2 cut(s) 227, 442
AfiI CCNNNNNNNGG 3 cut(s) 75, 515, 585
AgsI TTSAA 7 cut(s) 13, 265, 315, 598, 628, 707, 779
AjuI GAANNNNNNNTTGG 4 cut(s) 625, 657, 715, 747
AluBI AGCT 3 cut(s) 120, 475, 617
AluI AGCT 3 cut(s) 120, 475, 617
Alw21I GWGCWC 1 cut(s) 122
Alw26I GTCTC 3 cut(s) 46, 154, 413
AoxI GGCC 1 cut(s) 322
ApeKI GCWGC 2 cut(s) 617, 745
ApoI RAATTY 3 cut(s) 265, 701, 730
Asp700I GAANNNNTTC 2 cut(s) 348, 702
AspLEI GCGC 1 cut(s) 745
AspS9I GGNCC 1 cut(s) 285
AvaII GGWCC 1 cut(s) 285
BanI GGYRCC 1 cut(s) 718
BanII GRGCYC 1 cut(s) 122
Bbv12I GWGCWC 1 cut(s) 122
BbvI GCAGC 2 cut(s) 604, 732
BccI CCATC 4 cut(s) 391, 456, 474, 709
BceAI ACGGC 2 cut(s) 241, 756
BcoDI GTCTC 3 cut(s) 46, 154, 413
BfaI CTAG 3 cut(s) 399, 749, 784
BfmI CTRYAG 1 cut(s) 47
BfoI RGCGCY 1 cut(s) 746
BisI GCNGC 2 cut(s) 618, 746
BlsI GCNGC 2 cut(s) 619, 747
BmcAI AGTACT 2 cut(s) 227, 442
Bme18I GGWCC 1 cut(s) 285
BmgT120I GGNCC 1 cut(s) 285
BmiI GGNNCC 2 cut(s) 380, 720
BmsI GCATC 1 cut(s) 676
Bpu10I CCTNAGC 1 cut(s) 545
BpuEI CTTGAG 1 cut(s) 106
Bsa29I ATCGAT 1 cut(s) 39
BsaBI GATNNNNATC 1 cut(s) 41
BsaJI CCNNGG 1 cut(s) 584
BsaXI ACNNNNNCTCC 2 cut(s) 109, 139
Bsc4I CCNNNNNNNGG 3 cut(s) 75, 515, 585
Bse1I ACTGG 2 cut(s) 193, 233
Bse3DI GCAATG 1 cut(s) 325
Bse8I GATNNNNATC 1 cut(s) 41
BseCI ATCGAT 1 cut(s) 39
BseDI CCNNGG 1 cut(s) 584
BseGI GGATG 4 cut(s) 18, 274, 352, 466
BseJI GATNNNNATC 1 cut(s) 41
BseLI CCNNNNNNNGG 3 cut(s) 75, 515, 585
BseMI GCAATG 1 cut(s) 325
BseNI ACTGG 2 cut(s) 193, 233
BseXI GCAGC 2 cut(s) 604, 732
BshFI GGCC 1 cut(s) 324
BshNI GGYRCC 1 cut(s) 718
BshVI ATCGAT 1 cut(s) 39
BsiHKAI GWGCWC 1 cut(s) 122
BslI CCNNNNNNNGG 3 cut(s) 75, 515, 585
BsmAI GTCTC 3 cut(s) 46, 154, 413
BsmBI CGTCTC 2 cut(s) 154, 413
BsnI GGCC 1 cut(s) 324
Bsp1286I GDGCHC 1 cut(s) 122
Bsp143I GATC 1 cut(s) 36
BspACI CCGC 1 cut(s) 689
BspANI GGCC 1 cut(s) 324
BspDI ATCGAT 1 cut(s) 39
BspLI GGNNCC 2 cut(s) 380, 720
BspQI GCTCTTC 1 cut(s) 557
BspT107I GGYRCC 1 cut(s) 718
BsrDI GCAATG 1 cut(s) 325
BsrI ACTGG 2 cut(s) 193, 233
BssECI CCNNGG 1 cut(s) 584
BssMI GATC 1 cut(s) 36
BssT1I CCWWGG 1 cut(s) 584
Bst6I CTCTTC 2 cut(s) 411, 557
BstAPI GCANNNNNTGC 1 cut(s) 686
BstDEI CTNAG 2 cut(s) 545, 651
BstF5I GGATG 4 cut(s) 18, 274, 352, 466
BstH2I RGCGCY 1 cut(s) 746
BstHHI GCGC 1 cut(s) 745
BstKTI GATC 1 cut(s) 39
BstMAI GTCTC 3 cut(s) 46, 154, 413
BstMBI GATC 1 cut(s) 36
BstMWI GCNNNNNNNGC 1 cut(s) 686
BstSFI CTRYAG 1 cut(s) 47
BstV1I GCAGC 2 cut(s) 604, 732
Bsu15I ATCGAT 1 cut(s) 39
BsuRI GGCC 1 cut(s) 324
BsuTUI ATCGAT 1 cut(s) 39
BtsCI GGATG 4 cut(s) 18, 274, 352, 466
CfoI GCGC 1 cut(s) 745
Cfr13I GGNCC 1 cut(s) 285
ClaI ATCGAT 1 cut(s) 39
CseI GACGC 2 cut(s) 171, 430
Csp6I GTAC 2 cut(s) 226, 441
CviAII CATG 1 cut(s) 99
CviJI RGCY 6 cut(s) 120, 232, 256, 324, 475, 617
CviKI_1 RGCY 6 cut(s) 120, 232, 256, 324, 475, 617
CviQI GTAC 2 cut(s) 226, 441
DdeI CTNAG 2 cut(s) 545, 651
DpnI GATC 1 cut(s) 38
DpnII GATC 1 cut(s) 36
DraI TTTAAA 1 cut(s) 270
DrdI GACNNNNNNGTC 1 cut(s) 427
DseDI GACNNNNNNGTC 1 cut(s) 427
Eam1104I CTCTTC 2 cut(s) 411, 557
EarI CTCTTC 2 cut(s) 411, 557
Ecl136II GAGCTC 1 cut(s) 120
Eco130I CCWWGG 1 cut(s) 584
Eco24I GRGCYC 1 cut(s) 122
Eco32I GATATC 2 cut(s) 43, 176
Eco47I GGWCC 1 cut(s) 285
Eco53kI GAGCTC 1 cut(s) 120
Eco57I CTGAAG 1 cut(s) 45
EcoICRI GAGCTC 1 cut(s) 120
EcoRV GATATC 2 cut(s) 43, 176
EcoT14I CCWWGG 1 cut(s) 584
EcoT22I ATGCAT 1 cut(s) 374
EcoT38I GRGCYC 1 cut(s) 122
ErhI CCWWGG 1 cut(s) 584
Esp3I CGTCTC 2 cut(s) 154, 413
FaeI CATG 1 cut(s) 102
FatI CATG 1 cut(s) 98
FauI CCCGC 1 cut(s) 682
FauNDI CATATG 1 cut(s) 374
Fnu4HI GCNGC 2 cut(s) 618, 746
FokI GGATG 4 cut(s) 5, 261, 339, 453
FriOI GRGCYC 1 cut(s) 122
Fsp4HI GCNGC 2 cut(s) 618, 746
FspBI CTAG 3 cut(s) 399, 749, 784
GlaI GCGC 1 cut(s) 744
GluI GCNGC 2 cut(s) 618, 746
HaeII RGCGCY 1 cut(s) 746
HaeIII GGCC 1 cut(s) 324
HgaI GACGC 2 cut(s) 171, 430
HhaI GCGC 1 cut(s) 745
Hin1II CATG 1 cut(s) 102
Hin6I GCGC 1 cut(s) 743
HinP1I GCGC 1 cut(s) 743
HindIII AAGCTT 1 cut(s) 473
HinfI GANTC 4 cut(s) 403, 462, 520, 761
Hpy188I TCNGA 5 cut(s) 340, 393, 411, 433, 664
Hpy188III TCNNGA 1 cut(s) 707
HpyAV CCTTC 3 cut(s) 515, 622, 745
HpyCH4V TGCA 3 cut(s) 93, 372, 620
HpyF10VI GCNNNNNNNGC 1 cut(s) 686
HpyF3I CTNAG 2 cut(s) 545, 651
Hsp92II CATG 1 cut(s) 102
HspAI GCGC 1 cut(s) 743
Kzo9I GATC 1 cut(s) 36
LguI GCTCTTC 1 cut(s) 557
LmnI GCTCC 2 cut(s) 117, 170
LpnPI CCDG 6 cut(s) 36, 206, 214, 225, 301, 642
Lsp1109I GCAGC 2 cut(s) 604, 732
LweI GCATC 1 cut(s) 676
MaeI CTAG 3 cut(s) 399, 749, 784
MaeIII GTNAC 1 cut(s) 135
MalI GATC 1 cut(s) 38
MboI GATC 1 cut(s) 36
MboII GAAGA 4 cut(s) 38, 398, 574, 710
MhlI GDGCHC 1 cut(s) 122
MluCI AATT 6 cut(s) 7, 30, 265, 296, 701, 730
MlyI GAGTC 2 cut(s) 397, 529
MnlI CCTC 7 cut(s) 70, 208, 489, 526, 591, 751, 782
Mph1103I ATGCAT 1 cut(s) 374
MroXI GAANNNNTTC 2 cut(s) 348, 702
MseI TTAA 4 cut(s) 144, 269, 477, 590
MslI CAYNNNNRTG 1 cut(s) 491
MspA1I CMGCKG 1 cut(s) 617
MwoI GCNNNNNNNGC 1 cut(s) 686
NdeI CATATG 1 cut(s) 374
NdeII GATC 1 cut(s) 36
NlaIII CATG 1 cut(s) 102
NlaIV GGNNCC 2 cut(s) 380, 720
NmuCI GTSAC 1 cut(s) 135
NsiI ATGCAT 1 cut(s) 374
PciSI GCTCTTC 1 cut(s) 557
PdmI GAANNNNTTC 2 cut(s) 348, 702
PfeI GAWTC 2 cut(s) 462, 761
PkrI GCNGC 2 cut(s) 619, 747
PleI GAGTC 2 cut(s) 397, 528
PpsI GAGTC 2 cut(s) 397, 528
Psp124BI GAGCTC 1 cut(s) 122
PspN4I GGNNCC 2 cut(s) 380, 720
PspPI GGNCC 1 cut(s) 285
PvuII CAGCTG 1 cut(s) 617
RsaI GTAC 2 cut(s) 227, 442
RsaNI GTAC 2 cut(s) 226, 441
RseI CAYNNNNRTG 1 cut(s) 491
SacI GAGCTC 1 cut(s) 122
SapI GCTCTTC 1 cut(s) 557
SaqAI TTAA 4 cut(s) 144, 269, 477, 590
SatI GCNGC 2 cut(s) 618, 746
Sau3AI GATC 1 cut(s) 36
Sau96I GGNCC 1 cut(s) 285
ScaI AGTACT 2 cut(s) 227, 442
SchI GAGTC 2 cut(s) 397, 529
SduI GDGCHC 1 cut(s) 122
SetI ASST 6 cut(s) 86, 122, 477, 537, 619, 661
SfaNI GCATC 1 cut(s) 676
SfcI CTRYAG 1 cut(s) 47
SinI GGWCC 1 cut(s) 285
SmiMI CAYNNNNRTG 1 cut(s) 491
SmlI CTYRAG 1 cut(s) 121
SmoI CTYRAG 1 cut(s) 121
Sse9I AATT 6 cut(s) 7, 30, 265, 296, 701, 730
SsiI CCGC 1 cut(s) 689
SspI AATATT 1 cut(s) 415
SspMI CTAG 3 cut(s) 399, 749, 784
SstI GAGCTC 1 cut(s) 122
StyI CCWWGG 1 cut(s) 584
TaqI TCGA 1 cut(s) 39
TaqII GACCGA 1 cut(s) 123
TasI AATT 6 cut(s) 7, 30, 265, 296, 701, 730
TatI WGTACW 2 cut(s) 225, 440
TfiI GAWTC 2 cut(s) 462, 761
Tru1I TTAA 4 cut(s) 144, 269, 477, 590
Tru9I TTAA 4 cut(s) 144, 269, 477, 590
TseFI GTSAC 1 cut(s) 135
TseI GCWGC 2 cut(s) 617, 745
Tsp45I GTSAC 1 cut(s) 135
TspDTI ATGAA 3 cut(s) 222, 341, 357
VpaK11BI GGWCC 1 cut(s) 285
XapI RAATTY 3 cut(s) 265, 701, 730
XcmI CCANNNNNNNNNTGG 1 cut(s) 455
XmnI GAANNNNTTC 2 cut(s) 348, 702
XspI CTAG 3 cut(s) 399, 749, 784
ZrmI AGTACT 2 cut(s) 227, 442
Zsp2I ATGCAT 1 cut(s) 374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.