Rh2BG452000

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000234
Physical Location & Seq
Reverse (-)
63756468 .. 63764165
7698 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG452000.1

Sequence Viewer

Length: 924 bp
ATGGTGGGAGGAGAGACTATCCTCAAAATCCCTTTTATTCGGTTTAGGTGTGTATGCAAGTCATGGCGAGATTTGATTCGGAGCTCAAGTTTTGCTCGGTCACATCTTAATAGGAATGTAGCGAGACGCTCCAATAGATATCTGTATGCTTACCACCAGTCCTCAATAGATGAACCAGAACATTGTCAGTACTCACTTTTCTCTCCTGAAACATTTGAGCCGTGCTTGAATTTAAGACATCCCTTACGGACCAGAGAAAAATTGAGAATATATGGTTCAAGCAATGGCCTTGTGTGTATCTCTGATGAAAAACTTCATCCAAATAGTCGTATATGCATATGGAACCCATCTATTCGGAAGTCTAGGACTCTTCCGAATATTGAGACGCATTTGTCTGATATAAGGACTACCCATCTCTCGTTTGGATTCCATCCAGAGCTTGATGATTACATAGTTGTGAGGATTGTCCATCCTTATATGGGGAGTCCTGGAGTTGAGGTTTATACCACCCTTGGTACAAACTCTTGGAAGAGCATTCGGGTAACCCCTCCTTGGTTAAACTTCAATCGCATTTACTCCACAGCTGCATTTTTAAAGGGAACAGCATATTGGACTGCTAAGGCAGGTTCAGAATATAAGATTGTAGCATTTGATGCGGGAAGTGAAGAATTTCAAGAACTGATTGTGCCAAAGAAAAATTCCTTCACGGCGCTGCTAGTGTGCGAGGAATCACTTTGCCTCTTTCAATACGAGTGGGAATGGCTGGAATGGTCAGGAGAATGGACCATTGACTCATGGATAATGAAAGAGCAGTCTTTGAACAAGGTGGATTCTAGTTCACTCGAGAAAAGCGGATGCTATTTTCCACTCAGCTCTAGCATAGATAATGAACTAGTGAACTACTCATTGCATATATGGATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

307

Amino Acids

35.69

Weight (kDa)

7.6

Isoelectric Point (pI)

42.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 77 - 232 1.3e-09 KIB1-4 beta-propeller
FBA_3 PF08268 79 - 251 2.9e-16 F-box associated beta propeller domain
FBA_1 PF07734 93 - 275 5.3e-17 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000234)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18880 FvH4_2g02020 FvH4_2g02020 FvH4_2g37110 FvH4_6g33750 FvH4_6g33770 FvH4_6g34412 FvH4_7g08973
malus_domestica MD09G1191300.v1.1 MD09G1281000.v1.1 MD09G1281100.v1.1 MD09G1281200.v1.1 MD12G1050800.v1.1
prunus_persica Prupe.1G188300_v2.0.a1 Prupe.2G120700_v2.0.a1 Prupe.3G013300_v2.0.a1 Prupe.3G013500_v2.0.a1 Prupe.3G047100_v2.0.a1 Prupe.3G047200_v2.0.a1 Prupe.3G047300_v2.0.a1 Prupe.3G047400_v2.0.a1 Prupe.6G319100_v2.0.a1
pyrus_communis pycom09g18870 pycom11g17570 pycom12g04520 pycom14g04100
rosa_chinensis RchiOBHm_Chr1g0321721 RchiOBHm_Chr1g0321751 RchiOBHm_Chr1g0344471 RchiOBHm_Chr1g0344741 RchiOBHm_Chr1g0344891 RchiOBHm_Chr2g0143631 RchiOBHm_Chr2g0143641 RchiOBHm_Chr2g0144781 RchiOBHm_Chr3g0458821 RchiOBHm_Chr6g0245251 RchiOBHm_Chr6g0304681
rosa_laevigata RLG00000007681 RLG00000010222 RLG00000013320 RLG00000015313 RLG00000017431 RLG00000017439 RLG00000020055 RLG00000020056 RLG00000020112 RLG00000025133 RLG00000028872 RLG00000028881 RLG00000028884 RLG00000030407
rosa_multiflora Rmu_co8324891.1_g000001 Rmu_sc0000087.1_g000002 Rmu_sc0001288.1_g000009 Rmu_sc0001634.1_g000031 Rmu_sc0001942.1_g000032 Rmu_sc0003410.1_g000036 Rmu_sc0003909.1_g000002 Rmu_sc0004340.1_g000024 Rmu_sc0005308.1_g000008 Rmu_sc0005705.1_g000040 Rmu_sc0016560.1_g000002 Rmu_ssc0000167.1_g000005
rosa_roxburghii Rroxscaffold_2G00101580 Rroxscaffold_4G00309600 Rroxscaffold_4G00309710 Rroxscaffold_4G00327490 Rroxscaffold_5G00333360 Rroxscaffold_6G00420770 Rroxscaffold_7G00163600 Rroxscaffold_7G00215330
rosa_rugosa Rorug01G0174400 Rorug02G0213500 Rorug02G0384500 Rorug02G0384500 Rorug02G0384500 Rorug03G0032100 Rorug03G0301100 Rorug05G0513100
rosa_samantha Rh1AG045800 Rh1AG046600 Rh1AG191000 Rh1AG191200 Rh1AG192400 Rh1BG045100 Rh1BG157400 Rh1BG158200 Rh1CG048800 Rh1CG176300 Rh1CG177700 Rh1DG053100 Rh1DG055000 Rh1DG188800 Rh2AG180100 Rh2AG180200 Rh2AG436400 Rh2AG436500 Rh2AG441300 Rh2BG444000 Rh2BG444100 Rh2BG452000 Rh2CG422700 Rh2CG422800 Rh2CG428600 Rh2DG185900 Rh2DG186000 Rh2DG454300 Rh2DG454400 Rh2DG461700 Rh3AG089700 Rh3BG092600 Rh3CG092900 Rh3DG093600 Rh4AG005900 Rh4DG004900 Rh4DG010300 Rh4DG010400 Rh4DG230700 Rh6AG028300 Rh6AG445500 Rh6BG024500 Rh6BG456400 Rh6BG456500 Rh6CG022600 Rh6CG458300 Rh6CG458400 Rh6DG022600 Rh6DG445500
rosa_wichuraiana Rw1G004020 Rw1G004060 Rw1G004180 Rw1G015690 Rw1G015790 Rw2G014090 Rw2G035570 Rw2G035580 Rw2G036090 Rw3G007680 Rw6G002380 Rw6G038740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 391
Acc36I ACCTGC 1 cut(s) 614
AciI CCGC 2 cut(s) 656, 852
AcsI RAATTY 3 cut(s) 229, 668, 697
AfaI GTAC 2 cut(s) 191, 517
AfiI CCNNNNNNNGG 2 cut(s) 479, 552
AgsI TTSAA 6 cut(s) 229, 279, 565, 674, 746, 820
AhlI ACTAGT 1 cut(s) 892
AjnI CCWGG 1 cut(s) 487
AjuI GAANNNNNNNTTGG 4 cut(s) 592, 624, 682, 714
AluBI AGCT 4 cut(s) 84, 439, 584, 873
AluI AGCT 4 cut(s) 84, 439, 584, 873
Alw21I GWGCWC 1 cut(s) 86
Alw26I GTCTC 3 cut(s) 8, 118, 377
Ama87I CYCGRG 1 cut(s) 842
AoxI GGCC 1 cut(s) 286
ApeKI GCWGC 2 cut(s) 584, 712
ApoI RAATTY 3 cut(s) 229, 668, 697
Asp700I GAANNNNTTC 2 cut(s) 312, 669
AspLEI GCGC 1 cut(s) 712
AspS9I GGNCC 2 cut(s) 249, 783
AvaI CYCGRG 1 cut(s) 842
AvaII GGWCC 2 cut(s) 249, 783
BanII GRGCYC 1 cut(s) 86
Bbv12I GWGCWC 1 cut(s) 86
BbvI GCAGC 2 cut(s) 571, 699
BccI CCATC 4 cut(s) 355, 420, 438, 477
BceAI ACGGC 2 cut(s) 205, 723
BciT130I CCWGG 1 cut(s) 489
BcoDI GTCTC 3 cut(s) 8, 118, 377
BcuI ACTAGT 1 cut(s) 892
BfaI CTAG 5 cut(s) 363, 716, 834, 876, 893
BfoI RGCGCY 1 cut(s) 713
BfuAI ACCTGC 1 cut(s) 614
BisI GCNGC 2 cut(s) 585, 713
BlsI GCNGC 2 cut(s) 586, 714
BmcAI AGTACT 1 cut(s) 191
Bme1390I CCNGG 1 cut(s) 489
Bme18I GGWCC 2 cut(s) 249, 783
BmeT110I CYCGRG 1 cut(s) 842
BmgT120I GGNCC 2 cut(s) 249, 783
BmiI GGNNCC 1 cut(s) 344
BmrFI CCNGG 1 cut(s) 489
BmsI GCATC 2 cut(s) 643, 845
BpmI CTGGAG 1 cut(s) 510
Bpu10I CCTNAGC 1 cut(s) 618
BpuEI CTTGAG 1 cut(s) 70
BsaJI CCNNGG 2 cut(s) 511, 551
BsaXI ACNNNNNCTCC 4 cut(s) 73, 103, 483, 513
Bsc4I CCNNNNNNNGG 2 cut(s) 479, 552
Bse1I ACTGG 1 cut(s) 157
Bse3DI GCAATG 2 cut(s) 289, 905
BseBI CCWGG 1 cut(s) 489
BseDI CCNNGG 2 cut(s) 511, 551
BseGI GGATG 5 cut(s) 238, 316, 430, 469, 860
BseLI CCNNNNNNNGG 2 cut(s) 479, 552
BseMI GCAATG 2 cut(s) 289, 905
BseMII CTCAG 1 cut(s) 883
BseNI ACTGG 1 cut(s) 157
BseRI GAGGAG 1 cut(s) 24
BseXI GCAGC 2 cut(s) 571, 699
BshFI GGCC 1 cut(s) 288
BsiHKAI GWGCWC 1 cut(s) 86
BsiHKCI CYCGRG 1 cut(s) 842
BslI CCNNNNNNNGG 2 cut(s) 479, 552
BsmAI GTCTC 3 cut(s) 8, 118, 377
BsmBI CGTCTC 2 cut(s) 118, 377
BsmI GAATGC 1 cut(s) 534
BsnI GGCC 1 cut(s) 288
BsoBI CYCGRG 1 cut(s) 842
Bsp1286I GDGCHC 1 cut(s) 86
BspACI CCGC 2 cut(s) 656, 852
BspANI GGCC 1 cut(s) 288
BspCNI CTCAG 1 cut(s) 882
BspLI GGNNCC 1 cut(s) 344
BspMI ACCTGC 1 cut(s) 614
BspQI GCTCTTC 1 cut(s) 524
BsrDI GCAATG 2 cut(s) 289, 905
BsrI ACTGG 1 cut(s) 157
BssECI CCNNGG 2 cut(s) 511, 551
BssT1I CCWWGG 2 cut(s) 511, 551
Bst2UI CCWGG 1 cut(s) 489
Bst6I CTCTTC 2 cut(s) 375, 524
BstAPI GCANNNNNTGC 1 cut(s) 653
BstDEI CTNAG 2 cut(s) 618, 869
BstEII GGTNACC 1 cut(s) 541
BstF5I GGATG 5 cut(s) 238, 316, 430, 469, 860
BstH2I RGCGCY 1 cut(s) 713
BstHHI GCGC 1 cut(s) 712
BstMAI GTCTC 3 cut(s) 8, 118, 377
BstMWI GCNNNNNNNGC 1 cut(s) 653
BstNI CCWGG 1 cut(s) 489
BstPI GGTNACC 1 cut(s) 541
BstSCI CCNGG 1 cut(s) 487
BstV1I GCAGC 2 cut(s) 571, 699
BsuRI GGCC 1 cut(s) 288
BtsCI GGATG 5 cut(s) 238, 316, 430, 469, 860
BveI ACCTGC 1 cut(s) 614
CfoI GCGC 1 cut(s) 712
Cfr13I GGNCC 2 cut(s) 249, 783
CseI GACGC 2 cut(s) 135, 394
Csp6I GTAC 2 cut(s) 190, 516
CspCI CAANNNNNGTGG 2 cut(s) 734, 769
CviAII CATG 2 cut(s) 63, 795
CviJI RGCY 7 cut(s) 84, 220, 288, 439, 584, 763, 873
CviKI_1 RGCY 7 cut(s) 84, 220, 288, 439, 584, 763, 873
CviQI GTAC 2 cut(s) 190, 516
DdeI CTNAG 2 cut(s) 618, 869
DraI TTTAAA 1 cut(s) 594
DrdI GACNNNNNNGTC 1 cut(s) 391
DseDI GACNNNNNNGTC 1 cut(s) 391
Eam1104I CTCTTC 2 cut(s) 375, 524
EarI CTCTTC 2 cut(s) 375, 524
Ecl136II GAGCTC 1 cut(s) 84
Eco130I CCWWGG 2 cut(s) 511, 551
Eco24I GRGCYC 1 cut(s) 86
Eco32I GATATC 1 cut(s) 140
Eco47I GGWCC 2 cut(s) 249, 783
Eco53kI GAGCTC 1 cut(s) 84
Eco88I CYCGRG 1 cut(s) 842
Eco91I GGTNACC 1 cut(s) 541
EcoICRI GAGCTC 1 cut(s) 84
EcoO65I GGTNACC 1 cut(s) 541
EcoRII CCWGG 1 cut(s) 487
EcoRV GATATC 1 cut(s) 140
EcoT14I CCWWGG 2 cut(s) 511, 551
EcoT22I ATGCAT 1 cut(s) 338
EcoT38I GRGCYC 1 cut(s) 86
ErhI CCWWGG 2 cut(s) 511, 551
Esp3I CGTCTC 2 cut(s) 118, 377
FaeI CATG 2 cut(s) 66, 798
FatI CATG 2 cut(s) 62, 794
FauI CCCGC 1 cut(s) 649
FauNDI CATATG 1 cut(s) 338
Fnu4HI GCNGC 2 cut(s) 585, 713
FokI GGATG 5 cut(s) 225, 303, 417, 456, 867
FriOI GRGCYC 1 cut(s) 86
Fsp4HI GCNGC 2 cut(s) 585, 713
FspBI CTAG 5 cut(s) 363, 716, 834, 876, 893
GlaI GCGC 1 cut(s) 711
GluI GCNGC 2 cut(s) 585, 713
GsuI CTGGAG 1 cut(s) 510
HaeII RGCGCY 1 cut(s) 713
HaeIII GGCC 1 cut(s) 288
HgaI GACGC 2 cut(s) 135, 394
HhaI GCGC 1 cut(s) 712
Hin1II CATG 2 cut(s) 66, 798
Hin6I GCGC 1 cut(s) 710
HinP1I GCGC 1 cut(s) 710
HinfI GANTC 7 cut(s) 76, 367, 426, 484, 728, 791, 830
Hpy166II GTNNAC 2 cut(s) 839, 898
Hpy188I TCNGA 6 cut(s) 81, 304, 357, 375, 397, 631
Hpy188III TCNNGA 5 cut(s) 206, 434, 674, 774, 844
Hpy8I GTNNAC 2 cut(s) 839, 898
HpyAV CCTTC 1 cut(s) 712
HpyCH4V TGCA 4 cut(s) 57, 336, 587, 910
HpyF10VI GCNNNNNNNGC 1 cut(s) 653
HpyF3I CTNAG 2 cut(s) 618, 869
Hsp92II CATG 2 cut(s) 66, 798
HspAI GCGC 1 cut(s) 710
LguI GCTCTTC 1 cut(s) 524
LmnI GCTCC 2 cut(s) 81, 134
Lsp1109I GCAGC 2 cut(s) 571, 699
LweI GCATC 2 cut(s) 643, 845
MaeI CTAG 5 cut(s) 363, 716, 834, 876, 893
MaeIII GTNAC 2 cut(s) 99, 541
MboII GAAGA 3 cut(s) 362, 541, 677
MhlI GDGCHC 1 cut(s) 86
MluCI AATT 4 cut(s) 229, 260, 668, 697
MlyI GAGTC 3 cut(s) 361, 493, 785
MnlI CCTC 7 cut(s) 32, 172, 453, 490, 558, 718, 749
Mph1103I ATGCAT 1 cut(s) 338
MroXI GAANNNNTTC 2 cut(s) 312, 669
MseI TTAA 4 cut(s) 108, 233, 557, 593
MslI CAYNNNNRTG 1 cut(s) 455
MspA1I CMGCKG 1 cut(s) 584
MspR9I CCNGG 1 cut(s) 489
Mva1269I GAATGC 1 cut(s) 534
MvaI CCWGG 1 cut(s) 489
MwoI GCNNNNNNNGC 1 cut(s) 653
NdeI CATATG 1 cut(s) 338
NlaIII CATG 2 cut(s) 66, 798
NlaIV GGNNCC 1 cut(s) 344
NmuCI GTSAC 1 cut(s) 99
NsiI ATGCAT 1 cut(s) 338
PaeR7I CTCGAG 1 cut(s) 842
PciSI GCTCTTC 1 cut(s) 524
PctI GAATGC 1 cut(s) 534
PdmI GAANNNNTTC 2 cut(s) 312, 669
PfeI GAWTC 4 cut(s) 76, 426, 728, 830
PfoI TCCNGGA 1 cut(s) 487
PkrI GCNGC 2 cut(s) 586, 714
PleI GAGTC 3 cut(s) 361, 492, 785
PpsI GAGTC 3 cut(s) 361, 492, 785
Psp124BI GAGCTC 1 cut(s) 86
Psp6I CCWGG 1 cut(s) 487
PspEI GGTNACC 1 cut(s) 541
PspGI CCWGG 1 cut(s) 487
PspN4I GGNNCC 1 cut(s) 344
PspPI GGNCC 2 cut(s) 249, 783
PvuII CAGCTG 1 cut(s) 584
RsaI GTAC 2 cut(s) 191, 517
RsaNI GTAC 2 cut(s) 190, 516
RseI CAYNNNNRTG 1 cut(s) 455
SacI GAGCTC 1 cut(s) 86
SapI GCTCTTC 1 cut(s) 524
SaqAI TTAA 4 cut(s) 108, 233, 557, 593
SatI GCNGC 2 cut(s) 585, 713
Sau96I GGNCC 2 cut(s) 249, 783
ScaI AGTACT 1 cut(s) 191
SchI GAGTC 3 cut(s) 361, 493, 785
ScrFI CCNGG 1 cut(s) 489
SduI GDGCHC 1 cut(s) 86
SetI ASST 8 cut(s) 50, 86, 441, 501, 586, 628, 828, 875
SfaNI GCATC 2 cut(s) 643, 845
Sfr274I CTCGAG 1 cut(s) 842
SinI GGWCC 2 cut(s) 249, 783
SlaI CTCGAG 1 cut(s) 842
SmiMI CAYNNNNRTG 1 cut(s) 455
SmlI CTYRAG 2 cut(s) 85, 842
SmoI CTYRAG 2 cut(s) 85, 842
SpeI ACTAGT 1 cut(s) 892
Sse9I AATT 4 cut(s) 229, 260, 668, 697
SsiI CCGC 2 cut(s) 656, 852
SspI AATATT 1 cut(s) 379
SspMI CTAG 5 cut(s) 363, 716, 834, 876, 893
SstI GAGCTC 1 cut(s) 86
StyD4I CCNGG 1 cut(s) 487
StyI CCWWGG 2 cut(s) 511, 551
TaqI TCGA 1 cut(s) 843
TaqII GACCGA 1 cut(s) 87
TasI AATT 4 cut(s) 229, 260, 668, 697
TatI WGTACW 1 cut(s) 189
TfiI GAWTC 4 cut(s) 76, 426, 728, 830
Tru1I TTAA 4 cut(s) 108, 233, 557, 593
Tru9I TTAA 4 cut(s) 108, 233, 557, 593
TseFI GTSAC 1 cut(s) 99
TseI GCWGC 2 cut(s) 584, 712
Tsp45I GTSAC 1 cut(s) 99
TspDTI ATGAA 5 cut(s) 186, 305, 321, 818, 903
TspGWI ACGGA 1 cut(s) 262
VpaK11BI GGWCC 2 cut(s) 249, 783
XapI RAATTY 3 cut(s) 229, 668, 697
XcmI CCANNNNNNNNNTGG 1 cut(s) 419
XhoI CTCGAG 1 cut(s) 842
XmnI GAANNNNTTC 2 cut(s) 312, 669
XspI CTAG 5 cut(s) 363, 716, 834, 876, 893
ZrmI AGTACT 1 cut(s) 191
Zsp2I ATGCAT 1 cut(s) 338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.