Rh2AG436500

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000234
Physical Location & Seq
Reverse (-)
64755317 .. 64757172
1856 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG436500.1

Sequence Viewer

Length: 1137 bp
ATGATTTCCTTCAGGATGACGTCCACAATTTGTAAGAAAGAAATCTTGATGGACATCCTAGTAAGACTACCTGCAAAAACCCTAATACGGTTTTTATGTGCATGCAAAGCATGGAGTGATTTGATTAGCAGCCCAGATTTTGTCTCTACAAATCTTAACACAAATATCACAAAACGCAGAAATATGCATGTACTCTGCCTCCACCACCCGGATTTTAAACGGGTGGTCGATTTGGAGGATCCATATGTTAAACAAGCTCTTCAATGGTCTTTCTTTCACTATGAAACATTTGAGCAGGGCTTGGGGTTAAGCCATCCCTCAGGGAGCACAGAACATTATGTGATGTATGGCTCAAGCAATGGATTAGTTTGCATTTCGAATCCGGACGAAGTTTTGAATAGCAGGAGTCCTATAATCATATGGAACCCGTCCATTAAGAAATTTAAGACGCTTCCATCAACCACAAACAATAAATTTCGCTATATGTCCCTCCAATTTGGGTTCCATCCCGGGGTTAATGACTACAAGGTAGTAAGAATGATGCGGATCAACAAGGATGCCTTCGCAGTAGAAGTTTATTCTCTTAGCACCAACTCTTGGAAGATGATCGAAGAAATTCCTCCATGGTTAAAATGCAATTTTAAATATCTTGAAGGTACGTTCATAAATGGAGTAGCCTACCGAATTCTTGAGAAAGGTTTTATGTATAGCCTCATGTCCTTCGATTCGGGCAGTGAAGAATTCGAAGAATTCATACTACCAGAGTCCGTCATCAGTTCACATAATTTACATATCTACCCTTTCGAGGAAAAGCCTTGCTTGATTTTTCACTGGTATATTTCTGATGAGGAGGGCTTTGATAGAGGTGAGTTCTTTGTTTTTCAAGACAAACGTTGGAAACGTATGCACCCTTTTTACTATCCTTCGCATTGTTATCATACGATCGGGGTTAGTATAGATAATGAAGTGGTAATGGAGTCAAGAGACTACTGTAATGGTGTAGCAGATTTGTTTTTGGTGAATTACGAAACCAGGGAAGTTCGTGAAACAGGAATTAAGTTGGCCATCACGAGAGTTGGCTATAACGAACATTTCTATGTATTTACTTACGCAGAAAGTTTGGTATTACCGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

378

Amino Acids

44.29

Weight (kDa)

6.28

Isoelectric Point (pI)

54.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FBA_3 PF08268 110 - 358 1.4e-21 F-box associated beta propeller domain
FBA_1 PF07734 119 - 254 1.6e-14 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000234)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18880 FvH4_2g02020 FvH4_2g02020 FvH4_2g37110 FvH4_6g33750 FvH4_6g33770 FvH4_6g34412 FvH4_7g08973
malus_domestica MD09G1191300.v1.1 MD09G1281000.v1.1 MD09G1281100.v1.1 MD09G1281200.v1.1 MD12G1050800.v1.1
prunus_persica Prupe.1G188300_v2.0.a1 Prupe.2G120700_v2.0.a1 Prupe.3G013300_v2.0.a1 Prupe.3G013500_v2.0.a1 Prupe.3G047100_v2.0.a1 Prupe.3G047200_v2.0.a1 Prupe.3G047300_v2.0.a1 Prupe.3G047400_v2.0.a1 Prupe.6G319100_v2.0.a1
pyrus_communis pycom09g18870 pycom11g17570 pycom12g04520 pycom14g04100
rosa_chinensis RchiOBHm_Chr1g0321721 RchiOBHm_Chr1g0321751 RchiOBHm_Chr1g0344471 RchiOBHm_Chr1g0344741 RchiOBHm_Chr1g0344891 RchiOBHm_Chr2g0143631 RchiOBHm_Chr2g0143641 RchiOBHm_Chr2g0144781 RchiOBHm_Chr3g0458821 RchiOBHm_Chr6g0245251 RchiOBHm_Chr6g0304681
rosa_laevigata RLG00000007681 RLG00000010222 RLG00000013320 RLG00000015313 RLG00000017431 RLG00000017439 RLG00000020055 RLG00000020056 RLG00000020112 RLG00000025133 RLG00000028872 RLG00000028881 RLG00000028884 RLG00000030407
rosa_multiflora Rmu_co8324891.1_g000001 Rmu_sc0000087.1_g000002 Rmu_sc0001288.1_g000009 Rmu_sc0001634.1_g000031 Rmu_sc0001942.1_g000032 Rmu_sc0003410.1_g000036 Rmu_sc0003909.1_g000002 Rmu_sc0004340.1_g000024 Rmu_sc0005308.1_g000008 Rmu_sc0005705.1_g000040 Rmu_sc0016560.1_g000002 Rmu_ssc0000167.1_g000005
rosa_roxburghii Rroxscaffold_2G00101580 Rroxscaffold_4G00309600 Rroxscaffold_4G00309710 Rroxscaffold_4G00327490 Rroxscaffold_5G00333360 Rroxscaffold_6G00420770 Rroxscaffold_7G00163600 Rroxscaffold_7G00215330
rosa_rugosa Rorug01G0174400 Rorug02G0213500 Rorug02G0384500 Rorug02G0384500 Rorug02G0384500 Rorug03G0032100 Rorug03G0301100 Rorug05G0513100
rosa_samantha Rh1AG045800 Rh1AG046600 Rh1AG191000 Rh1AG191200 Rh1AG192400 Rh1BG045100 Rh1BG157400 Rh1BG158200 Rh1CG048800 Rh1CG176300 Rh1CG177700 Rh1DG053100 Rh1DG055000 Rh1DG188800 Rh2AG180100 Rh2AG180200 Rh2AG436400 Rh2AG436500 Rh2AG441300 Rh2BG444000 Rh2BG444100 Rh2BG452000 Rh2CG422700 Rh2CG422800 Rh2CG428600 Rh2DG185900 Rh2DG186000 Rh2DG454300 Rh2DG454400 Rh2DG461700 Rh3AG089700 Rh3BG092600 Rh3CG092900 Rh3DG093600 Rh4AG005900 Rh4DG004900 Rh4DG010300 Rh4DG010400 Rh4DG230700 Rh6AG028300 Rh6AG445500 Rh6BG024500 Rh6BG456400 Rh6BG456500 Rh6CG022600 Rh6CG458300 Rh6CG458400 Rh6DG022600 Rh6DG445500
rosa_wichuraiana Rw1G004020 Rw1G004060 Rw1G004180 Rw1G015690 Rw1G015790 Rw2G014090 Rw2G035570 Rw2G035580 Rw2G036090 Rw3G007680 Rw6G002380 Rw6G038740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 23
Acc36I ACCTGC 1 cut(s) 79
AccB7I CCANNNNNTGG 1 cut(s) 597
AccIII TCCGGA 1 cut(s) 382
AciI CCGC 1 cut(s) 544
AclI AACGTT 1 cut(s) 892
AclWI GGATC 3 cut(s) 233, 246, 554
AcoI YGGCCR 1 cut(s) 1062
AcsI RAATTY 6 cut(s) 440, 473, 615, 684, 740, 749
AcyI GRCGYC 1 cut(s) 20
AfaI GTAC 2 cut(s) 192, 658
AfiI CCNNNNNNNGG 5 cut(s) 87, 208, 511, 597, 805
AgsI TTSAA 4 cut(s) 263, 397, 653, 884
AjnI CCWGG 1 cut(s) 1031
AluBI AGCT 1 cut(s) 257
AluI AGCT 1 cut(s) 257
Alw21I GWGCWC 1 cut(s) 329
Alw26I GTCTC 2 cut(s) 148, 978
AlwI GGATC 3 cut(s) 233, 246, 554
Ama87I CYCGRG 1 cut(s) 509
Aor13HI TCCGGA 1 cut(s) 382
AoxI GGCC 1 cut(s) 1062
ApeKI GCWGC 1 cut(s) 129
ApoI RAATTY 6 cut(s) 440, 473, 615, 684, 740, 749
Asp700I GAANNNNTTC 1 cut(s) 615
AsuC2I CCSGG 3 cut(s) 209, 510, 511
AsuHPI GGTGA 2 cut(s) 878, 1030
AsuII TTCGAA 2 cut(s) 377, 744
AvaI CYCGRG 1 cut(s) 509
AxyI CCTNAGG 1 cut(s) 319
BalI TGGCCA 1 cut(s) 1064
BamHI GGATCC 1 cut(s) 238
BarI GAAGNNNNNNTAC 2 cut(s) 738, 770
BauI CACGAG 1 cut(s) 1069
Bbv12I GWGCWC 1 cut(s) 329
BbvI GCAGC 1 cut(s) 141
BccI CCATC 5 cut(s) 43, 321, 463, 513, 1073
BciT130I CCWGG 1 cut(s) 1033
BcnI CCSGG 3 cut(s) 209, 510, 511
BcoDI GTCTC 2 cut(s) 148, 978
BfaI CTAG 1 cut(s) 59
BfuAI ACCTGC 1 cut(s) 79
BisI GCNGC 1 cut(s) 130
BlsI GCNGC 1 cut(s) 131
Bme1390I CCNGG 4 cut(s) 209, 510, 511, 1033
BmeT110I CYCGRG 1 cut(s) 509
BmiI GGNNCC 3 cut(s) 240, 425, 503
BmrFI CCNGG 4 cut(s) 209, 510, 511, 1033
BmsI GCATC 2 cut(s) 531, 547
Bpu14I TTCGAA 2 cut(s) 377, 744
BpuEI CTTGAG 2 cut(s) 337, 710
BpuMI CCSGG 3 cut(s) 209, 510, 511
BsaBI GATNNNNATC 2 cut(s) 53, 545
BsaHI GRCGYC 1 cut(s) 20
BsaJI CCNNGG 4 cut(s) 509, 510, 623, 1032
BsaWI WCCGGW 1 cut(s) 382
BsaXI ACNNNNNCTCC 2 cut(s) 183, 213
Bsc4I CCNNNNNNNGG 5 cut(s) 87, 208, 511, 597, 805
Bse1I ACTGG 1 cut(s) 836
Bse21I CCTNAGG 1 cut(s) 319
Bse3DI GCAATG 1 cut(s) 364
Bse8I GATNNNNATC 2 cut(s) 53, 545
BseAI TCCGGA 1 cut(s) 382
BseBI CCWGG 1 cut(s) 1033
BseDI CCNNGG 4 cut(s) 509, 510, 623, 1032
BseGI GGATG 5 cut(s) 21, 54, 313, 505, 562
BseJI GATNNNNATC 2 cut(s) 53, 545
BseLI CCNNNNNNNGG 5 cut(s) 87, 208, 511, 597, 805
BseMI GCAATG 1 cut(s) 364
BseMII CTCAG 1 cut(s) 333
BseNI ACTGG 1 cut(s) 836
BseRI GAGGAG 1 cut(s) 863
BseXI GCAGC 1 cut(s) 141
Bsh1285I CGRYCG 1 cut(s) 945
BshFI GGCC 1 cut(s) 1064
BsiEI CGRYCG 1 cut(s) 945
BsiHKAI GWGCWC 1 cut(s) 329
BsiHKCI CYCGRG 1 cut(s) 509
BsiSI CCGG 3 cut(s) 209, 383, 510
BslFI GGGAC 1 cut(s) 472
BslI CCNNNNNNNGG 5 cut(s) 87, 208, 511, 597, 805
BsmAI GTCTC 2 cut(s) 148, 978
BsmFI GGGAC 1 cut(s) 472
BsnI GGCC 1 cut(s) 1064
BsoBI CYCGRG 1 cut(s) 509
Bsp119I TTCGAA 2 cut(s) 377, 744
Bsp1286I GDGCHC 1 cut(s) 329
Bsp13I TCCGGA 1 cut(s) 382
Bsp143I GATC 4 cut(s) 238, 546, 606, 942
Bsp19I CCATGG 1 cut(s) 623
BspACI CCGC 1 cut(s) 544
BspANI GGCC 1 cut(s) 1064
BspCNI CTCAG 1 cut(s) 332
BspEI TCCGGA 1 cut(s) 382
BspLI GGNNCC 3 cut(s) 240, 425, 503
BspMI ACCTGC 1 cut(s) 79
BspPI GGATC 3 cut(s) 233, 246, 554
BspQI GCTCTTC 1 cut(s) 264
BspT104I TTCGAA 2 cut(s) 377, 744
BsrDI GCAATG 1 cut(s) 364
BsrI ACTGG 1 cut(s) 836
BssECI CCNNGG 4 cut(s) 509, 510, 623, 1032
BssMI GATC 4 cut(s) 238, 546, 606, 942
BssNI GRCGYC 1 cut(s) 20
BssSI CACGAG 1 cut(s) 1069
BssT1I CCWWGG 1 cut(s) 623
Bst2BI CACGAG 1 cut(s) 1069
Bst2UI CCWGG 1 cut(s) 1033
Bst4CI ACNGT 2 cut(s) 90, 992
Bst6I CTCTTC 1 cut(s) 264
BstACI GRCGYC 1 cut(s) 20
BstBI TTCGAA 2 cut(s) 377, 744
BstC8I GCNNGC 1 cut(s) 103
BstDEI CTNAG 2 cut(s) 319, 584
BstDSI CCRYGG 1 cut(s) 623
BstF5I GGATG 5 cut(s) 21, 54, 313, 505, 562
BstKTI GATC 4 cut(s) 241, 549, 609, 945
BstMAI GTCTC 2 cut(s) 148, 978
BstMBI GATC 4 cut(s) 238, 546, 606, 942
BstMCI CGRYCG 1 cut(s) 945
BstMWI GCNNNNNNNGC 1 cut(s) 107
BstNI CCWGG 1 cut(s) 1033
BstNSI RCATGY 2 cut(s) 105, 191
BstSCI CCNGG 4 cut(s) 207, 508, 509, 1031
BstV1I GCAGC 1 cut(s) 141
BstX2I RGATCY 1 cut(s) 238
BstYI RGATCY 1 cut(s) 238
Bsu36I CCTNAGG 1 cut(s) 319
BsuRI GGCC 1 cut(s) 1064
BtgI CCRYGG 1 cut(s) 623
BtsCI GGATG 5 cut(s) 21, 54, 313, 505, 562
BtsI GCAGTG 1 cut(s) 739
BtsIMutI CAGTG 2 cut(s) 739, 829
BveI ACCTGC 1 cut(s) 79
Cac8I GCNNGC 1 cut(s) 103
Cfr9I CCCGGG 1 cut(s) 509
CseI GACGC 1 cut(s) 457
Csp6I GTAC 2 cut(s) 191, 657
CviAII CATG 5 cut(s) 102, 111, 188, 624, 715
CviQI GTAC 2 cut(s) 191, 657
DdeI CTNAG 2 cut(s) 319, 584
DpnI GATC 4 cut(s) 240, 548, 608, 944
DpnII GATC 4 cut(s) 238, 546, 606, 942
DraI TTTAAA 2 cut(s) 217, 643
EaeI YGGCCR 1 cut(s) 1062
Eam1104I CTCTTC 1 cut(s) 264
EarI CTCTTC 1 cut(s) 264
Eco130I CCWWGG 1 cut(s) 623
Eco81I CCTNAGG 1 cut(s) 319
Eco88I CYCGRG 1 cut(s) 509
EcoRI GAATTC 3 cut(s) 684, 740, 749
EcoRII CCWGG 1 cut(s) 1031
EcoT14I CCWWGG 1 cut(s) 623
EcoT22I ATGCAT 1 cut(s) 189
ErhI CCWWGG 1 cut(s) 623
FaeI CATG 5 cut(s) 105, 114, 191, 627, 718
FalI AAGNNNNNCTT 4 cut(s) 545, 577, 803, 835
FaqI GGGAC 1 cut(s) 472
FatI CATG 5 cut(s) 101, 110, 187, 623, 714
FauNDI CATATG 2 cut(s) 244, 419
Fnu4HI GCNGC 1 cut(s) 130
FokI GGATG 5 cut(s) 28, 41, 300, 492, 569
Fsp4HI GCNGC 1 cut(s) 130
FspBI CTAG 1 cut(s) 59
GluI GCNGC 1 cut(s) 130
HaeIII GGCC 1 cut(s) 1064
HapII CCGG 3 cut(s) 209, 383, 510
HgaI GACGC 1 cut(s) 457
Hin1I GRCGYC 1 cut(s) 20
Hin1II CATG 5 cut(s) 105, 114, 191, 627, 718
HinfI GANTC 5 cut(s) 379, 406, 725, 764, 977
HpaII CCGG 3 cut(s) 209, 383, 510
HphI GGTGA 2 cut(s) 878, 1030
Hpy166II GTNNAC 2 cut(s) 24, 779
Hpy188I TCNGA 1 cut(s) 844
Hpy188III TCNNGA 9 cut(s) 13, 46, 383, 650, 689, 884, 981, 1043, 1069
Hpy8I GTNNAC 2 cut(s) 24, 779
HpyAV CCTTC 5 cut(s) 19, 571, 647, 730, 933
HpyCH4III ACNGT 2 cut(s) 90, 992
HpyCH4IV ACGT 4 cut(s) 20, 659, 892, 901
HpyCH4V TGCA 7 cut(s) 74, 101, 105, 187, 372, 636, 907
HpyF10VI GCNNNNNNNGC 1 cut(s) 107
HpyF3I CTNAG 2 cut(s) 319, 584
HpySE526I ACGT 4 cut(s) 20, 659, 892, 901
Hsp92I GRCGYC 1 cut(s) 20
Hsp92II CATG 5 cut(s) 105, 114, 191, 627, 718
Kpn2I TCCGGA 1 cut(s) 382
Kzo9I GATC 4 cut(s) 238, 546, 606, 942
LguI GCTCTTC 1 cut(s) 264
LmnI GCTCC 1 cut(s) 324
Lsp1109I GCAGC 1 cut(s) 141
LweI GCATC 2 cut(s) 531, 547
MaeI CTAG 1 cut(s) 59
MaeII ACGT 4 cut(s) 20, 659, 892, 901
MalI GATC 4 cut(s) 240, 548, 608, 944
MboI GATC 4 cut(s) 238, 546, 606, 942
MboII GAAGA 5 cut(s) 251, 613, 623, 749, 758
MflI RGATCY 1 cut(s) 238
MhlI GDGCHC 1 cut(s) 329
MlsI TGGCCA 1 cut(s) 1064
MluNI TGGCCA 1 cut(s) 1064
MlyI GAGTC 3 cut(s) 415, 773, 986
MmeI TCCRAC 1 cut(s) 875
Mox20I TGGCCA 1 cut(s) 1064
Mph1103I ATGCAT 1 cut(s) 189
MroI TCCGGA 1 cut(s) 382
MroXI GAANNNNTTC 1 cut(s) 615
MscI TGGCCA 1 cut(s) 1064
MslI CAYNNNNRTG 1 cut(s) 1095
Msp20I TGGCCA 1 cut(s) 1064
MspI CCGG 3 cut(s) 209, 383, 510
MspR9I CCNGG 4 cut(s) 209, 510, 511, 1033
MvaI CCWGG 1 cut(s) 1033
MwoI GCNNNNNNNGC 1 cut(s) 107
NciI CCSGG 3 cut(s) 209, 510, 511
NcoI CCATGG 1 cut(s) 623
NdeI CATATG 2 cut(s) 244, 419
NdeII GATC 4 cut(s) 238, 546, 606, 942
NlaIII CATG 5 cut(s) 105, 114, 191, 627, 718
NlaIV GGNNCC 3 cut(s) 240, 425, 503
NsiI ATGCAT 1 cut(s) 189
NspI RCATGY 2 cut(s) 105, 191
NspV TTCGAA 2 cut(s) 377, 744
PaeI GCATGC 1 cut(s) 105
PciSI GCTCTTC 1 cut(s) 264
PcsI WCGNNNNNNNCGW 1 cut(s) 898
PdmI GAANNNNTTC 1 cut(s) 615
PfeI GAWTC 2 cut(s) 379, 725
PflMI CCANNNNNTGG 1 cut(s) 597
PkrI GCNGC 1 cut(s) 131
Ple19I CGATCG 1 cut(s) 945
PleI GAGTC 3 cut(s) 414, 772, 985
PpsI GAGTC 3 cut(s) 414, 772, 985
Psp1406I AACGTT 1 cut(s) 892
Psp6I CCWGG 1 cut(s) 1031
PspGI CCWGG 1 cut(s) 1031
PspN4I GGNNCC 3 cut(s) 240, 425, 503
PsuI RGATCY 1 cut(s) 238
PvuI CGATCG 1 cut(s) 945
RsaI GTAC 2 cut(s) 192, 658
RsaNI GTAC 2 cut(s) 191, 657
RseI CAYNNNNRTG 1 cut(s) 1095
SapI GCTCTTC 1 cut(s) 264
SatI GCNGC 1 cut(s) 130
Sau3AI GATC 4 cut(s) 238, 546, 606, 942
SchI GAGTC 3 cut(s) 415, 773, 986
ScrFI CCNGG 4 cut(s) 209, 510, 511, 1033
SduI GDGCHC 1 cut(s) 329
SfaNI GCATC 2 cut(s) 531, 547
SfuI TTCGAA 2 cut(s) 377, 744
SmaI CCCGGG 1 cut(s) 511
SmiMI CAYNNNNRTG 1 cut(s) 1095
SmlI CTYRAG 2 cut(s) 352, 689
SmoI CTYRAG 2 cut(s) 352, 689
SphI GCATGC 1 cut(s) 105
SsiI CCGC 1 cut(s) 544
SspMI CTAG 1 cut(s) 59
StyD4I CCNGG 4 cut(s) 207, 508, 509, 1031
StyI CCWWGG 1 cut(s) 623
TaaI ACNGT 2 cut(s) 90, 992
TaiI ACGT 4 cut(s) 23, 662, 895, 904
TaqI TCGA 6 cut(s) 228, 377, 609, 723, 744, 804
TatI WGTACW 1 cut(s) 190
TfiI GAWTC 2 cut(s) 379, 725
TscAI CASTG 2 cut(s) 739, 836
TseI GCWGC 1 cut(s) 129
TspDTI ATGAA 4 cut(s) 297, 652, 742, 978
TspGWI ACGGA 1 cut(s) 757
TspMI CCCGGG 1 cut(s) 509
TspRI CASTG 2 cut(s) 739, 836
Van91I CCANNNNNTGG 1 cut(s) 597
XapI RAATTY 6 cut(s) 440, 473, 615, 684, 740, 749
XceI RCATGY 2 cut(s) 105, 191
XmaI CCCGGG 1 cut(s) 509
XmnI GAANNNNTTC 1 cut(s) 615
XspI CTAG 1 cut(s) 59
ZraI GACGTC 1 cut(s) 21
Zsp2I ATGCAT 1 cut(s) 189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.